Biosorbents Removed Copper Heavy Metal from Agricultural Land Cultivated with Vigna radiata (Mung Bean)

Background . Heavy metals in excessive levels are hazardous to ecosystems as they can bioaccumulate in organisms, have toxic effects on biota, and even cause mortality in most life forms. Methodology . The present study consists of two studies; initially, different concentrations of copper were biosorbed by using 1 gm powder of various biosorbents such as orange peels (OP), pomegranate peels (POP), and peanut peels (PP). Furthermore, the biosorbed water was used to irrigate soil when the mung beans were cultivated. The growth parameters of plants growing under induced copper stress and treated with different bioabsorbed waters were also evaluated. Results . The results revealed that, among all biosorbents, the copper biosorption capacity of orange peels was maximum (90%), followed by pomegranate peel. The results of the second experiment exhibited that the plants irrigated with biosorbed water did not show metal toxicity. A remarkable increase in shoot length, shoot fresh weight, and shoot dry weight was observed (29.8 cm, 15.4g, and 14g, respectively) when exposed to biosorbent water with peanut peels+200 mg kg − 1 (Cu 2+ ). Similarly, pomegranate peel biosorbed water turned out to be an effective treatment to enhance root length, root fresh weight, and root dry weight (6.81cm, 4.07 g, and 2.66g, respectively) and resist against induced heavy metal stress conditions at higher concentration (200 mg kg − 1 ). Furthermore, orange peel biosorbed water elevated the total chlorophyll content and soluble sugar content in mung bean (1.56mg/g and 1.89mg/g). The highest tolerance index of mung bean plant grown under the stress of Cu 2+ metal was induced by orange peel biosorbed water. Conclusion . Biosorption is an environmentally friendly approach to mitigate heavy metals from the water. The studies showed that agricultural wastes have enough bioabsorption potential and should be used to absorb the heavy metal present in water.


Introduction
Mung bean (Vigna radiata) belongs to the family Fabaceae (Leguminosae); it is one of the nutritionally rich and nitrogen-xing summer pulse crops and is commonly grown worldwide particularly, having a short span of the life cycle (3-4 months). Essentially, the mung bean is a leguminous plant mostly cultivated for edible seeds [1]. e application of mung is wide as fodder, food, and green manure. It authenticates as an economical source of protein which is directly consumed by human beings; additionally, it enhances soil fertility. Mung bean seed chemical analysis revealed that, fundamentally, it possesses 24.9% protein, 1.3% fat, and 60% carbohydrates; it also comprises a fair amount of vital minerals such as phosphorous, calcium, iron, sodium, and potassium and, moreover, some vitamins such as riboflavin, thiamine, and vitamin A [2,3]. Mung bean plant growth rate is assessed annually. e shoot of mung bean is in the form of erect or semierect, and it could gain a height of 0.15-1.25 m. e root system fabrication is composed of a slightly hairy and developed root system. However, the leaves are in alternate venation, either be trifoliate with ellipsoidal leaves to form ovate leaflets, which can grow up to 5-18 cm long and 3-15 cm wide [4]. e flowers develop in clusters, which vary from 4 to 30 at the top of the plant, and it is papilionaceous, greenish, or pale yellow. Mung bean pods are of considerable length, cylindrical, and fuzzy which might be covered with shaded layers. eir seeds may vary from 7 to 20, although it is small-sized. However, it could be cube-shaped or ellipsoidal seeds. e seeds are of many colors: generally, it is green; however, they can also be pale yellow, brown, olive, purplish brown, or black and mottled or ridged in appearance [4]. e roots of mung bean plants are narcotic and diuretic and used to treat different diseases such as abscesses, bone pain, dropsy, headache, and inflammation. e seeds of this plant are used as emollients, astringents, laxative snacks, nerve tonics, and styptics. ey are useful for treating scabies, gonorrhea, aches, hemorrhoids, heart problems, constipation, and memory weakness [4]. In some countries, including Pakistan, India, Bangladesh, ailand, and Korea, mung bean is important as a summer legume crop. In South Asia, the demand for pulses is enhancing, but since the early 1960s, acreage and yield production have not increased [5,6].
Heavy metals are metallic elements that have a comparatively high density and are venomous in fewer amounts; these heavy metals include arsenic (As), mercury (Hg), lead (Pb), cadmium (Cd), chromium (Cr), and thallium (TI). Some trace elements are also known as heavy metals such as copper (Cu), selenium (Se), and zinc (Zn). ey are compulsory to maintain the metabolism of the body, but they are toxic in higher concentrations [7,8]. Excessive amounts of heavy metals are harmful as they threaten ecosystems due to their bioaccumulation in organisms, cause toxic effects in biota, and even cause death in most living organisms. Heavy metal contamination of water is caused by the addition of organic compounds, heavy metals, sludge, and sewage organisms; it also includes fungi, algae, bacteria, and hazardous material and chemicals [9]. Water pollution due to heavy metals has been recognized as a worldwide threat since the beginning of the industrial revolt and addition of hydrocarbons, heavy metals, inorganic anions, pesticides, nutrient loads, and microplastic [10]. It occurs when microorganisms and harmful chemicals from household trash and industry come into touch with water sources, run away, or seep into the water table and freshwater supplies [11]. eir high concentrations contaminate the water and ultimately living life forms. e prevalence of coliform bacteria is indicated by the contamination of animal and human wastes. Owing to drainage in watercourses, such as rivers, lakes, and streams, bacteria development and dispersal are at their height during the rainy season. Waterborne infections spread due to inadequate treatment. In Pakistan, the potable sanitation and drainage systems run simultaneously, resulting in leaks and water quality degradation due to intermixing [12].
Copper being a metal is very toxic; it significantly affects agriculture and the environment. It comes from mining, smelting, and agricultural and industrial wastes [13]. However, it is essential for the soil, whereas its excess amount negatively affects the growth of plants. It helps plant growth by providing structural strength to the plants, and it also participates in the photosynthesis process in the electron transport chain. It also helps to catalyze the cell wall metabolism and hormone signaling. For the higher plants, copper is a well-known necessary micronutrient among other metals, but a higher amount of this metal causes a lack of other essential nutrients by competitive prohibiting at absorption sites. Besides, it reduces the enzyme of production and suppresses the functioning of the membrane [13][14][15]. In Pakistan, the discharge of wastes and untreated effluents of industries into freshwater bodies has become a serious problem. e number of metals, particularly copper (Cu 2+ ) included in these industrial wastes, is considerably high in amount. When these are taken up or absorbed in higher concentrations by crop plants, it creates a significant problem. ey inhibit the metabolic process and reduce crop production by entering the plant's body; these metals are subsequently incorporated into the food chain and cause liver and brain disorders [16,17]. e term biosorption implies a direct interaction between the metal sorbate and biosorbents. e diffusion of the metal ion from the bulk solution to the active sites of the microorganism, in the nonliving biomass, occurs predominantly by passive transport mechanisms. Initially, metal ions diffuse to the surface of the microbial cell where they bind to active sites on the cell surface formed by the presence of different chemical groups such as hydroxyl, carboxylate, amine, and phosphate that relate to the metal ions' affinity [18,19].
Recently, it widely focused on various natural solid supports, which can remove contaminants from contaminated water at a low cost. ese include chemical precipitation, membrane filtration, ion exchange, electrolysis, coagulation, solvent extraction, reverse osmosis, and electrocoagulation. However, these mentioned techniques have several disadvantages, such as incomplete removal of pollutants, are regarded as low selectivity, use of high reagent and chemical, energy-consuming, the production of other toxic wastes that need to be operated carefully, and mostly high cost [20]. Conversely, the use of biological methods such as bioremediation, bioaccumulation, or biosorption for the exclusion of heavy metals or utilization of microorganisms and plants for remediation function is thus achievable key to reducing the heavy metal contamination [21]. Among various remediation methods, the biosorption through agriculture waste seems to be more effective in huge 2 International Journal of Agronomy scale applications compared to the bioaccumulation process because the microbes require the addition of nutrients for their active uptake of heavy metals, which increases the biological oxygen required [22,23]. Agricultural wastes could be assumed to be low-cost adsorbents because they are abundant in nature, low-priced, and plentiful, require a little transformation, and are also useful and convenient materials. To remove copper material, many agricultural waste materials and some peels of fruits and vegetables have been used as adsorbents (biosorbents) [24,25]. erefore, the main purpose of the current research work was to check three distinct biosorbents which are pomegranate, peanut, and orange peels for copper (Cu 2+ ) metal biosorption from an aqueous solution and examine biomaterial accumulation of heavy metals. Moreover, the impact of Cu 2+ stress on morphology and biochemical profiling of the mung bean plants was also evaluated.

Preparation of Biosorbents.
Biosorbent peels (orange, peanut, and pomegranate) were collected from various local markets in Lahore (Punjab, Pakistan). For two days, the biomaterials were kept sun-dry on the roof. By sun drying, the mass and volume of biomass were reduced. e color of the biosorbent peels was also changed after drying in the sun. Subsequently, when the sun drying is done, the biomass was decently washed with the help of tap water and once more with distilled water. After cleaning, the biomass was left for drying for a night in an oven at 60°C. After oven drying, the biomaterials (peels) were also transformed into small pieces. e biomaterials' (biosorbent peels) tiny pieces were ground in a kitchen grinder to convert them into fine powder. After crushing the biosorbent peels, a fine powder was prepared, as shown in Figure 1 [26].

Experimental Trials.
In the Erlenmeyer flasks (250 ml), about 100 ml of each concentration such as 0, 50, 100, 150, and 200 ppm of Cu 2+ was taken. e pH of the solution was adjusted with a strong base and acid (0.1 M NaOH and 0.1 M HCl) by using a pH meter (model 3505, Jenway). Into each metal solution, about 1 g of each biosorbent powder (orange peel, peanut peel, and pomegranate peel powder) was added. e mixture (adsorbent + metal) holding flasks were placed on an orbital shaker for 20 minutes at 120 rpm. e filtration of the solution was done by using Whatman No. 42 after the stirring process. To find out the number of residual metal ions' concentrations, the filtrate was analyzed on an atomic absorption spectrophotometer.

Biosorption Data Evaluation.
Accumulation of the quantity of Cu 2+ by the biomaterial (peels) was calculated by distinguishing between the initial and final quantity present in the solution after equilibration with the biomaterial using the given formula. Likewise, the biosorption efficiency (E) for each experiment was calculated by using the following equation: e concentrations of metallic ions are shown in the following formula; the initial concentration (mg L −1 ) is represented by (C i ), whereas the final concentration (absorption) of metallic ion (mg L −1 ) is represented by (C f ). e diagrammatic experimental flow can be seen in Figure 2 [28].

Soil Sterilization and Metal
Spiking. Cotton was soaked in formalin solution, and plugs were inserted in the soil at different points for its sterilization (fumigation). After inserting the formalin-soaked cotton plugs into the soil, it is roofed with a plastic sheet and left for the next 7 days. To completely evaporate formalin fumes, all the cotton plugs and sheets were removed from the soil after 7 days to leave it open for another 7 days. Finally, the soil was sieved in pots (mung bean-sized, 2 mm) and also utilized for metal spiking. e soil was enriched with copper metal by spraying each concentration of an aqueous solution of (V) 50, 100, 150, and 200 mg kg −1 and left for 15 days (followed by resieving and crushing of the soil). Furthermore, Cu 2+ metal spiked in soil was also assessed with the following modified formula [29]: required water � total weight of soil required W t × % age saturation of soil 100 . (2) International Journal of Agronomy 2.6. Seed Sowing. After metal spiking into the soil, four healthy and sterilized seeds of mung bean were sown in plastic pots. In the shaded glasshouse, those soil-containing plastic pots were placed (25°C ± 3; 12-hour photoperiod). Furthermore, by irrigating with tap water, the dampness of the soil was also maintained. e details of the treatments are given in Table 1. Furthermore, the experiment was conducted using a complete randomized design with 3 repetitions. After the tenth day of germination of the seed, all the plants were analyzed to determine the changes in morphological and height (growth) parameters, the length of the roots and shoots, and the total biomass (fresh and dry weight). Meanwhile, antioxidant enzyme activity and the physiological changes in the plants (mung beans) were examined after 10 days of seed germination. Moreover, after the 10 th day of sprouting, the accumulation of metal by the plant was also estimated.

Physiological
Assays. After 7 days of germination of mung bean, various physiological parameters such as total chlorophyll content [30], soluble sugar [31], and total protein content [32] were estimated.

Growth
Assay. After 10 days, Vigna radiata (mung bean) plants were sampled. e plants were uprooted carefully from pots at the time of harvesting, then washed under (tap) running water, and desiccated with the help of blotting paper after uprooting and washing. Plant shoots and roots were also separated. e length and fresh weight of each plant in cm and gm were recorded, respectively. Both roots and shoots were dried in an oven for 24 hours (at 80°C) and reweighed for the dry weight measurement.

Metal Toxicity Assessment.
Morphological symptoms such as the stunted growth of an entire plant, yellow patches on leaves, decrease in vegetative growth, and root reduction were also observed after 10 days of sowing mung bean plants in Cu 2+ contaminated soils.

Statistical Analysis.
e assessment of metal absorption capacity of biosorbent, toxic influence of copper and treated water with biosorbents on seed and seedling growth of mung

Biosorption Batch.
For copper (Cu 2+ ) metal biosorption from an aqueous solution, three distinct biosorbents that are pomegranate, peanut, and orange peels were tested. e examined biomaterials exhibited tremendous results for the accumulation of heavy metals.

Accumulation of Cu 2+ by Pomegranate Peels.
Adsorption experiments were carried out in batches at room temperature. Contact time, pH, initial metal ion concentration, and adsorbent concentrations (0-200 ppm) were studied. e results showed that pomegranate peels absorbed about 40-110 ppm of copper from the aqueous solution of 50 and 200 ppm copper (Cu 2+ ) metal. Moreover, the efficiency of the tested biosorbents was 80-55% from 50 to 200 ppm of copper (Cu 2+ ) metal in an aqueous (water solvent) solution, as illustrated in Table 2.

Accumulation of Cu 2+ by Peanut Peels.
It was found that the peanut peels absorbed about 20-73 ppm of copper from the aqueous solution of 50 and 200 ppm copper (Cu 2+ ) metal. Furthermore, the efficiency of the tested biosorbent was 40-37% against 50-200 ppm of copper (Cu 2+ ) metal in an aqueous solution (Table 3).

Accumulation of Cu 2+ by Orange Peels.
It was found that the orange peels absorbed about 45-155 ppm of copper from the aqueous solution of 50 and 200 ppm copper (Cu 2+ ) metal. Additionally, the efficiency of the tested biosorbents was 90-78% against 50-200 ppm of copper (Cu 2+ ) metal in an aqueous solution (Table 4). e pot experiment was conducted in a controlled environment in which the impact of biosorbent water with different biosorbents including peanut peels (PP), pomegranate peels (POP), and orange peels (OP) was studied. Tremendous variations in shoot length parameters, root length parameters, and biomass (dry mass and fresh mass) were observed.

Metal Toxicity and Growth Parameters.
e impact of Cu 2+ stress on the morphology of the mung bean plant is depicted in Figure 3. e results revealed the deleterious effect of higher doses of copper metal (100-200 mg kg −1 ) on the growth of mung bean plants. e stunted growths with yellowing of leaves were observed at 100-200 mg kg −1 of Cu 2+ as compared to (−ve) control. e implementation of biosorbed water exhibited better growth in mung bean plants. No retarded growth symptoms were observed in mung bean plants that were treated with biosorbed water.

Shoot Parameters
e impact of various biosorbed waters on the shoot length of mung beans is illustrated in Figure 4. After observations, it was noticed that the shoot length of the mung bean plant was reduced at 50 mg kg −1 in comparison with negative control, while significant reduction of shoot length was observed at 150 and 200 mg kg −1 concentration of Cu 2+ (16.6 cm and 19.7 cm).
e biosorbed water has momentously enhanced the shoot length of the plant even under induced stress of heavy metal at higher concentration. Highest shoot length was observed in the plant treated with peanut peel biosorbed water +200 mg/kg of Cu 2+ (29.8 cm). is was followed by the plant treated with pomegranate peel biosorbed water +100 mg/kg (28 cm). e orange peel biosorbed water (OPBW) elevated the shoot length in the plant exposed to 200 mg/kg of Cu 2+ (24.2 cm).

Shoot Fresh Weight.
e results also revealed that the rising concentration of Cu 2+ (50-200 mg kg −1 ) adversely affected the shoot fresh weight of mung bean plant as compared to the negative control, whereas the biosorbed water reduced the heavy metal accumulation in plant and increased the shoot fresh weight even at 200 mg kg −1 concentration of heavy metal. e plant treated with peanut peel biosorbed water +200 mg kg −1 Cu 2+ showed the maximum shoot biomass formation (15.4 g). Similar results were also observed in the plant treated with pomegranate peel biosorbed water +100 mg kg −1 Cu 2+ (12.9 g). A moderate level of increase in shoot fresh weight of mung bean was also induced by the orange peel biosorbed water ( Figure 5).

Shoot Dry Weight.
e shoot dry weight of mung bean was considerably reduced by more than 2-folds with the exposure to a higher concentration of Cu 2+ (100-200 mg kg −1 ) as compared to the negative control. However, the shoot dry weight of the mung bean plant was improved (14 g) by treatment with peanut peel biosorbed water under induced Cu 2+ stress conditions (200 mg kg −1 ).
is was followed by the plant treated with pomegranate peel biosorbed water +100 mg kg −1 Cu 2+ (11.8 g). e orange peel biosorbed water also enhanced a considerable level of shoot dry biomass of mung bean ( Figure 6).

Root Parameters
e impact of various biosorbed waters and concentrations of heavy metal on the root length of mung bean is illustrated in Figure 7. A moderate level of reduction was observed in the root length of the mung bean plant after exposure to 50 mg kg −1 of heavy metal, while momentous reduction was examined at higher concentration of copper heavy metal (100-200 mg kg −1 ). Almost 3-fold reduction was calculated in root length of plants exposed to 100 mg kg −1 of Cu 2+ as compared to the negative control.
Furthermore, the results revealed that the pomegranate peel biosorbed water (POPBW) significantly inhibited the accumulation of Cu 2+ in plant even at higher concentration (200 mg/kg) and promoted the root growth and development of mung bean (6.81 cm) as compared to the respective control.
e peanut peel biosorbed water (PPBW) also promoted rooting (5.89 cm) in plant germinating under induced heavy metal stress conditions (200 mg kg −1 ). Furthermore, the orange peel biosorbed water (OPBW) was effective for the plants growing under lower concentration of heavy metal (Figure 7).

Root Fresh Weight.
Along with the length of roots, different concentrations of the heavy metals also adversely affected the root fresh biomass accumulation. e least root fresh weight (0.11 g) was noticed in the plant grown under the stress of higher concentration of Cu 2+ heavy metal (200 mg kg −1 ), while these plants when grown in the presence of pomegranate peel biosorbed water had the maximum root biomass formation (4.07 g). Moreover, the soil treated with PPBW after being stressed with 200 mg kg −1 concentration of Cu 2+ enhanced the root growth and fresh biomass (3.22 g) (Figure 8).

Root Dry
Weight. Similar to root fresh weight, the higher concentrations of the heavy metals also adversely affected the dry root biomass of mung bean plant. e results revealed that the root dry weight of the mung bean plant was reduced by 2-3-folds with the increasing concentration of Cu 2+ as compared to the negative control, whereas the soil treated with POPBW in the presence of heavy metal at 200 mg kg −1 concentration significantly improved the root dry biomass of mung bean plant by 2.5-folds (2.66 g) as compared to the plant grown under heavy metal stress only. e root dry weight of mung bean plant was also enhanced by the treatment with PPBW as compared to plant growth under the stress of copper only (150 mg kg −1 ). Moreover, the root dry weight of mung bean was considerably increased to 2.13 g when treated with OPBW along with the presence of lower concentration of heavy metal (Figure 9).

Total Chlorophyll Content.
e impact of various biosorbed waters on the chlorophyll content of mung bean is illustrated in Figure 10. e heavy metal stress retarded not only the growth of the plants but also the physiological parameters significantly. A 3-fold decline in the total chlorophyll content was detected in plant stressed with higher concentration of heavy metal (200 mg kg −1 ) as compared to the negative control. e biosorbed waters were not effective enough against higher concentration of heavy metals to promote the level of chlorophyll pigment in plants, though a moderate level of increase was detected in plants treated with biosorbed waters and low level of heavy metal stress (200 mg kg −1 ). e maximum level of total chlorophyll content (1.56 mg/g) was detected in plants treated with orange peel biosorbed water + 50 mg kg −1 of copper ( Figure 10).

Soluble Sugar Content.
e soluble sugar content of the plant was affected with induced heavy metal stress, but the treatment with biosorbed water limited the accumulation of heavy metals and their toxic effects on the mung bean plant. e biosorbed water promoted the accumulation of total soluble sugar content in the plant when treated in the presence of minimal level of heavy metal (50 mg kg −1 ). e maximum soluble sugar content (1.89 mg/g) was detected in mung bean plant germinated under orange peel biosorbed water + 50 mg kg −1 of Cu 2+ (Figure 11).

Total Protein Content.
It is contingent on the situation that the response of the plant to the stress state determines    International Journal of Agronomy 7 whether total protein content will be enhanced or decreased. When plants are stressed, their total protein content may rise as a result of the generation of stress-related proteins. Similar results were also observed in the present study; the total protein content of the plant significantly increased with the raise in the concentration of induced heavy metal stress. A 2-3-fold increase of total protein content was detected in plants germinated in the presence of higher concentration of heavy metal (100, 150, and 200 mg kg −1 ) as compared to the negative control. e lowest level of total protein content was detected in the plant developed in the presence of peanut peel biosorbed water +200 mg kg −1 of Cu 2+ (0.34 mg/g) (Figure 12).

Tolerance Index of Mung Bean Plant.
It was found that the tolerance index of mung beans was 75-45% when grown in metal stress alone (50-200 mg kg −1 ), whereas the tested index of mung bean plants by POPBW and PPBW was 122-395% and 77-213%, respectively. However, the application of OPBW increased the level of tolerance index of the mung bean plant by 136-744% ( Figure 13).
have a lesser metal concentration (<100 mg l −1 ) [36]. Some researchers reported that it was possible to remove various heavy metals from agricultural wastes, and the removal capacities of these agricultural wastes were significantly affected by initial metal concentration [36]. While the heavy metal removal methods are based on the conventional principle of ion exchange, coagulation, chemical precipitation, membrane filtration technology, and biosorption, which are evolved in the earlier period, the risk of generating secondary pollutants by these methods is of great concern. Of all these methods above, the biosorption method is simple and relatively cost-effective and reduces the potential of yielding needless chemicals. It has been reported that biosorption is a promising technique for heavy metal adsorption from an aqueous environment, mainly when adsorbents are derived from biological sources: lingocellulose carbonaceous compounds, plant wastes, fruit and vegetable peels, natural resources, and agricultural wastes [37].
Although the toxic effects of copper were considerably adverse in plants, more uptake of Cu in plants causes significant negative effects that range from physiological and morphological to molecular levels, and these negative effects remain evident at all stages of plant growth. So, the higher concentration of Cu metal in various plants altered plant morphology root and shoot elongation. As the application of Cu increased in each treatment (10 mM and above), the shoot length and root length of the mung bean plant were similarly decreased as compared to the negative control [38].
For the optimal growth of plants, at concentrations higher than those required, Cu inhibits the growth of plants and interferes with important cellular processes such as respiration and photosynthesis. Plants grown in the presence of a higher amount of Cu 2+ usually show a reduction in biomass and chlorotic symptoms. Lower chlorophyll content, alterations of chloroplast structure, and thylakoid membrane composition were found in leaves under such growth situations. In particular, degradation of stroma, lamellae, and grana stacking, increase in the number and size of plastoglobuli, and appearance of intrathylakoidal inclusions were observed. Cu 2+ interferes with the photosynthetic machinery that modifies the pigments and proteins composition of photosynthetic membranes.
thylakoid membranes were also observed. As a result of such modifications, variation of PSII membrane fluidity was also found. Yruela, proposed that the processes induced by Cu2+ could involve either the destruction of the oxygen-evolving complex polypeptide composition or the contact with ions that are essential for the proper working of the complex such as Mn, Ca, and Cl, etc. that are essential for the proper working of the complex such as Mn, Ca, and Cl [39]. It is well known that, in the nonenzymatic chemical reaction between superoxide (O − 2 ) and H 2 O 2 , Cu acts as a transitional metal and speeds up the configuration of hydroxyl radicals (OH . ) (Haber-Weiss reaction) [40].
Hence, the presence of Cu 2+ in excess amounts can cause oxidative stress in plants and subsequently increase the antioxidant responses due to the increased production of highly toxic oxygen free radicals. Accordingly, it was observed that excess Cu 2+ in plants led to oxidative stress-inducing changes in the activity and content of some components of the antioxidative pathways such as ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), superoxide dismutases (SODs), and guaiacol peroxidase. e antioxidant responses were observed in leaves and roots being both Cu-concentration dependent and time-dependent. e ascorbate-glutathione cycle has been reported to be involved in response to excess Cu 2+ [41].

Conclusion
Heavy metal contamination of water has become alarmingly more important in recent years. e focus of present research is on developing a viable solution to either avoid or decrease heavy metal contamination to a manageable level. Heavy metal pollution of water bodies may only be controlled by decreasing direct discharge into the water stream. e most extensively used traditional techniques for removing heavy metals have a number of drawbacks, including high capital and operating costs, incompatibility with smallscale industries, and inefficiency. One of the most promising methods for eliminating heavy metals from wastewater is adsorption. e adsorption technique works efficiently even at very minimal concentrations. erefore, the use of lowcost agricultural wastes is promoted for the removal of heavy metals.  Figure 13: Tolerance index of mung bean plant grown under the stress of Cu 2+ metal and biosorbed water after 15 days of seed germination. T1, negative control; T2, 50 mg kg −1 ; T3, pomegranate peel biosorbed water +50 mg kg −1 ; T4, peanut peel biosorbed water +50 mg kg −1 ; T5, orange peel biosorbed water +50 mg kg −1 ; T6, 100 mg kg −1 ; T7, pomegranate peel biosorbed water +100 mg kg −1 ; T8, peanut peel biosorbed water +100 mg kg −1 ; T9, orange peel biosorbed water +100 mg kg −1 ; T10, 150 mg kg −1 ; T11, pomegranate peel biosorbed water +150 mg kg −1 ; T12, peanut peel biosorbed water +150 mg kg −1 ; T13, orange peel biosorbed water +150 mg kg −1 ; T14, 200 mg kg −1 ; T15, pomegranate peel biosorbed water +200 mg kg −1 ; T16, peanut peel biosorbed water +200 mg kg −1 ; T17, orange peel biosorbed water +200 mg kg −1 . Vertical bars (I): standard error (mean of 3 replicates). Alphabets on vertical bars: significant difference (P ≤ 0.05) evaluated by the LSD test.