A Comparative Study on Alvogyl and a Mixture of Black Seed Oil and Powder for Alveolar Osteitis: A Randomized Double-Blind Controlled Clinical Trial

Introduction Alveolar osteitis (AO) is the most common complication faced by exodontia patients and is usually seen 24–74 hours after tooth extraction, heralded by severe throbbing pain. Nigella sativa is commonly known as black seed known to have anti-inflammatory and antibacterial properties along with other reparative properties that enhance bone formation. This study aimed to evaluate and compare the effects of Alvogyl and a mixture of Nigella sativa powder and oil in the treatment of dry sockets. Materials and Methods Sixty patients above the age of 18 and below 70 years, from both genders, who underwent extraction of teeth and were clinically diagnosed with a dry socket at the clinic of the College of Dentistry, Jouf University, Saudi Arabia, were included in this study. Pain scores were assessed after placement of the dressing at the following intervals: 5 minutes, 30 minutes, 60 minutes, 2nd day, 4th day, and 7th day. Patients were randomly allocated to three groups, namely, Group 1 (Alvogyl), Group 2 (mixture of Nigella sativa's powder and oil), and Group 3 (control). Pain relief and healing of the socket were compared between the three groups. The collected data were subjected to statistical analysis through Spearman's correlation test, independent t-test, ANOVA, and post hoc test. Results A mixture of Nigella sativa powder and oil showed a statistically significant difference in relieving pain compared to the Alvogyl group. A mixture of Nigella sativa's powder and oil required fewer dressings when compared to the Alvogyl group. Conclusion A mixture of Nigella sativa powder and oil is the more efficacious dressing material for the management of dry sockets compared to Alvogyl. It provides immediate and complete pain relief and fewer numbers of repeated visits.


Introduction
Alveolar osteitis or "dry socket" is the most common complication faced by patients following tooth extraction, usually seen after 24-74 hours [1,2]. e entity was labeled as 'dry socket' or 'alveolar osteitis' in 1896 by Crawford and is defined as an acute inflammation of the alveolar bone observed after two to four days around the site of the extracted tooth socket heralded by severe throbbing pain and the disintegration of the blood clot formed inside the extraction socket that is often filled with food debris [3][4][5].
Several previous studies reported diverse incidences of dry socket ranging from 5 to 30% in surgical removal of impacted mandibular third molars and between 1 and 4% in simple extraction cases of permanent teeth [6,7]. e exact cause of dry socket is not precisely understood. Nevertheless, numerous denominators have been implicated, including age, gender, oral contraceptive use, tobacco cigarette smoking, tooth location, bone and soft tissue injury from problematic extractions, poor oral hygiene, and volume of vasoconstriction administered along with local anesthetics used, operative and postoperative management, and operator skill [2]. e local fibrinolytic activity is implicated as the main reason leading to dry socket [6]. e surge in fibrinolytic activity after extraction may subsequently lead to premature disintegration of the intraalveolar clot [2]. e fibrinolysis is the consequence of plasminogen pathway activation, initiated via direct physiologic activators after alveolar osteoid cells or secondary activator substances secreted by bacteria [8].
e exact pathogenesis of dry socket in association with tobacco cigarette smoking is to date unidentified. e largely accepted and dominant hypothesis suggests that the sucking motion associated with cigarette smoking causes mechanical clot dislodgement [2,3]. e treatment of this painful condition is wide-ranging including washing and flooding the empty tooth socket with a copious amount of normal saline and or placement of intrasocket medication, namely, Alvogyl, zinc oxide eugenol, chlorhexidine, metronidazole, olive oil-black seed paste, and honey [9,10].
Despite the availability of various medications for the treatment of dry-socket pain, not a single treatment option provides immediate and complete relief from symptoms. Subsequently, the tooth socket heals secondarily in ten to fourteen days with variable severity of pain continued throughout this healing period [10,11]. To enhance the efficacy of healthcare, the World Health Organization (WHO) has encouraged developing countries to integrate the use of therapeutic plants since more than two-thirds of their population depends on the use of natural remedies and traditional herbs for disease treatment [12,13].
Complementary and alternative medicine (CAM) is the term for medical products and practices that are not part of standard medical care. ere is an increasing interest towards the use of CAM in the field of medicine [14,15]. Patients with a variety of chronic conditions are using CAM to help cope with their disease [16][17][18]. e CAM products (Ferula assa-foetida oleo-gum resin) have been actively used in the field of dentistry to treat and prevent oral diseases related to oral mucosa and dental caries [19,20]. One such plant is Nigella sativa commonly known as black seed, black cumin, black caraway, kalojeera, kalonji, or kalonji [21]. Its chemical composition contains volatile and nonvolatile oils in addition to many other active ingredients including proteins, alkaloids, coumarines, saponins, minerals, carbohydrates, phenolic compounds, and steroidal compounds. Many studies have been conducted on the effects of Nigella sativa seed extracts on various body systems in vitro or in vivo. e pharmacological investigation of the seed extracts reveals a broad spectrum of activities including antidiabetic, anti-inflammatory, and osteoreparative properties that enhance bone formation [22].
One of the treatment options that is commonly used to treat patients suffering from the dry socket is Alvogyl. Alvogyl consists of active ingredients such as iodoform (antibacterial) and butamben (anesthetic) [23]. Alvogyl has been successfully studied in patients as it is known to provide relief from pain and discomfort in patients suffering from dry sockets [24]. Furthermore, Alvogyl has been compared with neocone and zinc oxide eugenol, with Alvogyl being superior to both of the agents [25].
Considering the abovementioned there is a need to investigate the efficacy of Nagilla Sativa on the postextraction complications of dry socket. To the best of our knowledge, this is the first comparative study of olive oilblack seed oil for the treatment of dry socket at the College of Dentistry, University of Jouf, Kingdom of Saudi Arabia, and is aimed to find out the best and most effective medication for the treatment of dry socket at this institution.

Study Setting, Sample Size, and Subjects Inclusion Criteria.
e OpenEpi software was used to calculate the sample size. Considering the mean value of 2.567 ± 2.678 [23] for dry socket pain severity, the power of the test was 80%. A confidence interval (CI) of 95%, and a margin of error of 5%. e estimated sample size for each of the three groups was 20 participants. erefore, a total of 60 patients were included in the study. In this comparative study, patients were selected from the dental clinics of the College of Dentistry, Jouf University, Sakaka, based on the following predetermined inclusion/exclusion criteria.

Inclusion Criteria
(1) Patients over the age of 18, from both genders, who had their teeth extracted and were clinically diagnosed with dry socket

Study Design.
Patients clinically diagnosed with alveolar osteitis were assigned numbers from first to the sixtieth patient and randomly allocated via lottery to three groups named as follows: (1) Alvogyl (Septodont, Saint-Maur-des-Fosses, France) (2) mixture of Nigella sativa's powder and oil (Kalonji Oil, Nigella sativa, Organic Pure Oil, Jeddah, Saudi Arabia), and (3) normal saline rinse as a control. Both the patient and the investigator assessing the treatment outcome were blinded from identifying the medicament dressing used for the group. e medication used was placed in unmarked identical bottles and saline in a covered syringe without the original label on them and marked as 1, 2, and 3.

Outcomes.
Patients were allowed to continue their oral analgesic medication, namely, ibuprofen 200 mg, 400 mg, or 600 mg twice daily, depending upon the severity of initial pain upon diagnosis. Pain relief was recorded and compared between the groups on a visual analog pain scale at every appointment. e intraalveolar medication was repeated until the postoperative pain symptoms subsided. Patients were reviewed at 5 minutes, 30 minutes, and 60 minutes after dressing.
e patient was requested to note daily pain records on a (0-10) visual analog scale, with zero representing no pain and ten representing the worst pain. e patients were requested to note daily pain records on a (0-10) visual analog scale for 7 days. ey also recorded any harmful effects of medication and advised to immediately call the investigator about any problem in the due course. If no side effects were noted, the treatment option was repeated a maximum of four times over two weeks to assess the complete effect of the medication in case complete relief was not achieved the first time.
e total time required for complete healing and the number of repeated sessions for each medication till complete relief from all symptoms were recorded.

Data Analysis.
e data was analyzed in SPSS-25 (Statistical Package for Social Sciences, version 25, IBM, Chicago, Illinois). A descriptive analysis was carried out to calculate the mean and standard deviation, percentage, and frequency of quantitative and qualitative variables such as age, gender, scores of the VAS scale, values for different study groups assigned, and the severity of pain categories. e independent t-test was used to check for gender disparities. ANOVA and the post hoc test were used to compare the outcomes of different groups assigned. Spearman's correlation test was used to evaluate the association of age and gender with the mean VAS scores of patients in each group. e pvalue of ≤0.05 was considered statistically significant.

Results
is randomized controlled trial consisted of sixty patients that were randomized into 3 groups as follows: Group 1, Alvogyl; Group 2, black seed; and Group 3, control, as presented in Figure 1. e mean age of the patients in each group was as follows: Group 1, 41.70; Group 2, 53.15; and Group 3, 49.30. About the gender, the distribution of the males and females in each group was as follows: Group 1, 11 and 9; Group 2, 10 and 10; and Group 3, 8 and 12. As for the comparison of age and gender with VAS pain scores amongst the groups, age and Group 1 patient VAS scores had a significant relationship (pvalue � 0.047), and gender and Group 2 patients also had a significant relationship (pvalue � 0.037), as presented in Table 1.
e mean pain scores of the patients before the placement of the intrasocket in all of the three groups are presented in Table 2. In this study, the incidence of dry socket was found in 16 (26.6%) patients after anterior teeth extractions and 44 (73.3%) patients after posterior teeth extractions in both upper and lower arches. Moreover, 26 (43.3%) of the dry socket cases were found in the maxilla and 44 (56.6%) in the mandible. e mean postmedication VAS pain score of patients in all the three groups was noted at the following time intervals: 5 minutes postmedication, 30 minutes postmedication, 60 minutes postmedication, 2 nd day postmedication, 4 th day postmedication, and 7th day postmedication. e majority of patients reported a decrease in pain scores in both groups 1 and 2, respectively, over time, as presented in Figure 2. e patients of Group 3 did not report a significant decrease in pain at 5 minutes postmedication. At 30 minutes and 60 minutes postmedication, a decrease in pain score was seen in patients belonging to groups 1 and 2, but the decrease was more significant in Group 2. However, patients in Group 3 did not experience a significant decrease in pain at the same interval. From the 2 nd day postmedication, all of the patients belonging to Group 2 were pain-free as compared to Group 1 where patients were still experiencing pain. On the 4th day postmedication, patients belonging to Group 1 still suffered minimal pain which disappeared completely in all of the patients. Patients belonging to Group 3 reported minimal to no pain on the 7 th day postmedication, as presented in Table 2.
e intergroup comparison through the application of the ANOVA test showed a significant difference between the mean scores of the three groups (A and B, p � 0.031), (A and International Journal of Clinical Practice 3 C, p � 0.011), and (B and C, p � 0.001), respectively, as presented in Table 3. Furthermore, the post hoc test indicated that patients treated with normal saline alone showed a high intensity of dry socket pain (p � 0.001) as compared to the Alvogyl and Nigella sativa powder and oil groups. Additionally, the least VAS scores were found in the Nigella Sativa powder and oil group compared to Alvogyl. Regarding the gender of the patients, an independent t-test was used to evaluate the difference between the VAS scores of males and females. No significant difference was found between the genders of all the groups (pvalue � 0.525), as presented in Table 4.

Discussion
Alveolar osteitis is one of the most common complications that arise as a postoperative complication associated frequently with the extraction of mandibular third molars. Since the pain of dry socket as experienced by the patients is severe and excruciating, this mandates timely management to relieve the discomfort and pain of the patients. Different risk factors are associated with a greater tendency to develop dry sockets such as smoking, use of oral contraceptives, female gender, suppression of the immune system, and traumatic extractions.
In our study, most of the patients suffered from severe pain using a visual analog scale when they were diagnosed to suffer from dry socket. Such findings have previously been reported in a study by Hawker et al., where patients who were suffering from dry socket reported severe dental pain. Since the bone around the socket is exposed to the oral environment with supplemental action of the bacteria, these factors then lead to the severe pain of the dry socket [26].
Firstly, in our study, patients who were assigned to Alvogyl dressing treatment in the socket reported a mild decrease in pain scores that were still significant, causing patients' pain and discomfort. Such findings have also been reported in a study by Faizel et al., who concluded that patients assigned with Alvogyl dressing experienced a decrease in the level of pain [25]. One of the ingredients of

International Journal of Clinical Practice
Alvogyl is eugenol which has sedative, antibacterial, and anodyne effects. Moreover, Alvogyl also contains butamben which is an anesthetic substance along with iodoform which is antibacterial [18]. ese properties possessed by Alvogyl make it suitable for patients suffering from dry sockets. However, one study by Abdull Gaffar et al. reported that the use of Alvogyl in the sockets of patients resulted in delayed healing and inflammation of the socket [27]. Secondly, in our study, we applied black seed liquid with powder in the sockets of patients suffering from dry sockets. In our study, we found that the pain relief of the patients in this group was significantly less as compared to Alvogyl immediately after its application. Patients belonging to black seed dressing groups were pain-free on the 2nd postmedication day. Dalimunte et al. found that the application of black seed in the sockets of the patients resulted in a significantly higher period of wound healing with 12% black cumin to have the most optimum effect on the healing of the socket [28]. One of the components of black seed is thymoquinone. One study by Syafriadi et al. reports that the use of thymoquinone postextraction sockets of diabetic induced rats resulted in an improvement of reepithelization at the margin of the sockets [29]. Hence, further studies are required to evaluate the use of thymoquinone as a preventive measure in high-risk patients that might develop dry sockets such as diabetic patients and smokers. Furthermore, it has been found that the use of powder of black seed in postextraction cases of patients resulted in more radiopacity with complete disappearance of the lamina dura in a time duration of 6 weeks along with complete epithelization [30].
In our study, the severity of pain between males and females was equal with no significant relationship between them. ese results contrast with studies in the literature that report higher severity of pain in females as compared to males [31]. Moreover, the female gender has been known to be a risk factor for the potential development of dry socket [32].
In the literature, different treatment options are available for the treatment of dry socket. Platelet-rich fibrin (PRF) has been used to treat dry sockets with a study reporting an early reduction in pain levels experienced by patients with minimal need of analgesic intake [33]. Moreover, honey has also been used to treat patients with dry sockets due to a significant reduction in inflammation, discomfort, pain, and hyperemia [34,35]. Other therapies can have a significant influence on the oral environment. e use of ozone and laser photodynamic therapies can modify clinical and microbiological parameters in periodontal patients, and they could also have an effect in the response to the technique described in the present report [36,37]. As in our study, it was found that a mixture of black seeds oil and powder can be effectively used as an alternative to Alvogyl in patients suffering from dry socket. All these different methods of treatment should be considered in future clinical trials to further evaluate the effectiveness of each of them in treating alveolar osteitis.
Dry socket is a condition that has a wide choice of treatment options such as Alvogyl, honey, zinc oxide eugenol, and black seed. Despite the strengths of this study such as regular follow-up of the patients and reporting of the pain scores, this study was met with some limitations. Firstly, the sample size of the patients in each group was small, and lastly, patients might not have followed the temporary cessation of habits during the healing period of sockets such as smoking.

Conclusion
e use of Alvogyl resulted in a gradual decrease in pain scores; however, black seed powder with oil resulted in immediate pain relief for the patients, being more effective than Alvogyl and the use of normal saline irrigation alone. Hence, a black seed oil mixture can be used effectively in patients suffering from alveolar osteitis.

Data Availability
e raw data used to support the findings of this study are included within the article.

Disclosure
ZAK and MKA are corresponding authors.

Conflicts of Interest
e authors declare that they have no conflicts of interest.

Authors' Contributions
ZAK, NP, AL, RI, AF, MKA, FV, and NA planned and designed the present work. NA, AM, ZAK, NP, and RI were responsible for realizing the work. NA, RI, AL, and MKA were responsible for the data acquisition and analysis. AM, FV, AL, and MKA drafted and revised the manuscript. ZAK, AM, FV, MKA, RI, and NA approved the final version of the manuscript. All authors read and approved the final manuscript. ZAK and MKA contributed equally to this work.