Association of Transferable Quinolone Resistance Determinant qnrB19 with Extended-Spectrum β-Lactamases in Salmonella Give and Salmonella Heidelberg in Venezuela

Four nontyphoidal Salmonella strains with resistance to extended-spectrum cephalosporins and nonclassical quinolone resistance phenotype were studied. Two S. Give were isolated from pediatric patients with acute gastroenteritis, and two S. Heidelberg were recovered from raw chicken meat. Phenotypic characterization included antimicrobial susceptibility testing and detection of extended-spectrum β-lactamases (ESBLs) by the double-disc synergy method. The detection of quinolone resistance-determining regions (QRDR) of gyrA, gyrB, and gyrC genes, bla ESBLs genes, and plasmid-mediated quinolone resistance (PMQR) determinants was carried out by molecular methods. Plasmid analysis included Southern blot and restriction patterns. Transferability of resistance genes was examined by transformation. bla TEM-1 + bla SHV-12 genes were detected in S. Give SG9611 and bla TEM-1 + bla CTX-M-2 in the other three strains: S. Give SG9811, S. Heidelberg SH7511, and SH7911. Regardless of origin and serovars, the qnrB19 gene was detected in the 4 strains studied. All determinants of resistance were localized in plasmids and successfully transferred by transformation. This study highlights the circulation of qnrB19 associated with bla TEM-1, bla SHV-12, and bla CTX-M-2 in S. Give and S. Heidelberg in Venezuela. The recognition of factors associated with increasing resistance and the study of the molecular mechanisms involved can lead to a more focused use of antimicrobial agents.


Introduction
Nontyphoidal Salmonella (NTS) are one of the major causes of foodborne infections related to the ingestion of contaminated animal food products in humans [1]. In most cases, these infections are confined to the gastrointestinal tract and are self-limiting. However, for immunocompromised and/or elderly patients, as well as for invasive or prolonged infections, antibiotic treatment is recommended [2]. Fluoroquinolones and extended-spectrum -lactams are the firstchoice agents for these cases but the increase of the multidrug resistance (MDR) Salmonella strains reduces the available treatment options [1][2][3][4][5].
The emergence of Salmonella spp. isolates that display resistance to extended-spectrum -lactams is mediated by plasmids and is an increasing public health concern [3][4][5]. The resistance to fluoroquinolones is typically mediated by alterations in the target enzymes DNA gyrase and topoisomerase IV or changes in drug entry and efflux. Also, three plasmid-mediated mechanisms conferring decreased susceptibility to ciprofloxacin have been recently described: QepA efflux, Aac(6 )-Ib-cr aminoglycoside acetyltransferase, and QNR proteins (qnrA, qnrB, qnrC, qnrD, and qnrS) [6,7]. The qnr determinants encode pentapeptide repeat proteins, which are thought to bind to DNA gyrase, protecting it from quinolones; aac(6 )-lb-cr encodes a variant aminoglycoside acetyltransferase that modifies those fluoroquinolones that have a piperazinyl moiety, such as ciprofloxacin and norfloxacin, while qepA encodes a major facilitator efflux pump [6][7][8]. Several studies have shown the coexistence of plasmidmediated quinolone resistance (PMQR) determinants with extended-spectrum -lactamases (ESBL) on the same plasmid or cross-species/genera transferability of the plasmids [6,9]. Selective pressure exerted by fluoroquinolones may be a driving force leading to the emergence and spread of isolates that carry not only PMQR determinants but also ESBL. Thus, close linkage between different resistance determinants may lead to high prevalence of MDR Salmonella strains under antibiotic-specific selective pressure [4,5,8]. In fact, the emergence of the association between PMQR and ESBL is an issue that narrows the usage of valuable antibiotics in managing NTS infections [1,5,9].
Epidemiologic studies of more than 2,300 NTS strains from human cases and animals in North America, Europe, and Asia have reported the prevalence of qnrA, qnrB, qnrS, and aac(6 )-lb-cr genes in 0.2%, 1.0%, 2.4%, and 6.4%, respectively [6]. On the other hand, in a previous study carried out by our team from 2005 to 2007 and in 2008, we found a prevalence of qnrB19 (4.7%) in 117 NTS strains isolated from feces of pediatric patients and raw chicken meat [10]. Nevertheless, molecular and epidemiologic information on mechanisms of resistance in Salmonella enterica in Venezuela is scarce [10,11]. The aim of this study was to determine the coexistence of PMQR determinant qnrB19 and ESBL in S. Give and S. Heidelberg isolates from clinical sample and raw chicken meat in Mérida, Venezuela.

Salmonella Strains.
In 2011, four NTS strains expressing a remarkable type of multidrug resistance were studied. These strains were selected from a collection of the Molecular Microbiology Laboratory of the Pharmacy and Bioanalysis Faculty of The Andes University, Mérida, Venezuela, based on resistance to extended-spectrum cephalosporins and an unusual quinolone resistance phenotype with reduced susceptibility to ciprofloxacin and associated susceptibility to nalidixic acid. Two S. Give (SG9611 and SG9811) were isolated from stool specimens obtained from pediatric patients with acute gastroenteritis, and two S. Heidelberg (SH7511 and SH7911) were recovered from raw chicken meat.

Detection of Mutations in the Topoisomerase
Genes gyrA, gyrB, and parC. PCR was used to amplify the quinolone resistance-determining regions (QRDR) of target genes to identify mutations in each isolate. DNA was prepared, and the QRDR of gyrA, gyrB, and parC were amplified with previously described primers and protocols [13].

Detection of -Lactamase Genes.
TEM , SHV , and group CTX-M genes were detected by PCR using previously described primers and protocols [16].
All amplification products were purified (PCR-Accuprep kit Bioneer), and their nucleotide sequencing was performed with the 3730XL genetic analyzer (Applied Biosystems, CA, USA). Nucleotide and amino acid sequence alignments were analyzed using the Basic Local Alignment Search Tool (BLAST) suite of programs (http://blast .ncbi.nlm.nih.gov/Blast.cgi).

Plasmid Analysis.
Plasmid DNA was extracted using the QIAprep spin miniprep kit (Qiagen Inc., USA). The presence of ESBLs and PMQR determinants was ensured using Southern blot analysis on nylon membranes as described for the dot blot hybridization [17]. These plasmids were analyzed by restriction mapping using the HindIII and PstI enzyme (Promega, Madison, WI, USA) to observe the restriction patterns, and the resulting fragment sizes were determined by gel electrophoresis.

Transformation Experiment.
ESBL and Qnr-harboring plasmids were transferred into E. coli HB101 competent cells by electroporation (Eppendorf model 2510, Germany) using approximately 0.5 g of the plasmid DNA preparation under conditions recommended by manufacturer. Mueller Hinton agar supplemented with 8 g/mL cefotaxime and 0.06 g/mL ciprofloxacin was used for selection of transformants. The presence of ESBLs and qnr genes in the transformants was confirmed by antimicrobial susceptibility and PCR assays. Results were compared with those of the corresponding donor strains.

Antimicrobial Susceptibility Testing.
MICs of selected antibiotic for S. Give and S. Heidelberg strains and their transformants are presented in Table 1. All strains were resistant to all tested extended-spectrum cephalosporins and monobactams. By using clavulanic acid, an 8-to 10fold reduction of MICs of cefotaxime and ceftazidime was observed. A positive double-disk test also confirmed ESBL production. These strains remained fully susceptible to cefoxitin, carbapenems, and amikacin. S. Give SG9811 and S. Heidelberg SH7911 were resistant to gentamicin (32 g/mL and >64 g/mL, resp.) while S. Give SG9611 was resistant to tobramycin (16 g/mL). ESBL and quinolone-resistance-harboring plasmids were successfully transferred by transformation from all parental strains to the recipient E. coli HB101 strain. Negligible differences were found among the MICs of the transformants and their Salmonella donors. Only S. Heidelberg SH7511 and SH7911 strains, showing a phenotype of intermediate susceptibility to gentamicin and tobramycin (8 g/mL), respectively, produced transformant cells (7511trf and 7911trf) clearly defined as resistant to gentamicin (16 g/mL) and sensitive to tobramycin (4 g/mL).

Plasmid Analysis.
Plasmid purified from 9811trf, 7511trf and 7911trf of ∼19 kb exhibited similar restriction pattern generated by single as well as double digestion HindIII and PstI, whereas p9611trf of ∼13 kb had a unique restriction pattern. However, Southern blot experiments confirmed that ESBL and qnr alleles were present in all isolated plasmids (data not shown).

Detection of -Lactamases and qnr
Genes. PCR amplification, using specific primers for TEM , SHV , and group CTX-M , and sequencing analysis, allowed us to identify TEM-1 + SHV-12 in S. Give SG9611 and TEM-1 + CTX-M-2 in the other three strains: S. Give SG9811, S. Heidelberg SH7511, and S. Heidelberg SH7911. Regardless of origin and serovars, the qnrB gene was detected in the 4 strains studied (Table 2). Sequence analysis of the amplification product revealed the qnrB19 variant with a 99% degree of identity, corresponding to the nucleotide sequence reference GenBank accession no. EU432277. No mutations were identified in the QRDR of the gyrA, gyrB, and parC genes.

Discussion
Since 2007, we have been reporting the presence of NTS producing gastroenteritis in pediatric patients from urban areas and in asymptomatic children from indigenous communities in Venezuela [11,18,19]. In these studies, Salmonella strains remained susceptible to cephalosporins and fluoroquinolones. Subsequently, in 2010, S. Heidelberg and S. Enteritidis strains isolated from food showed production of ESBL not associated with resistance to fluoroquinolones [20]. Recently, we also reported the first description of the occurrence of the PMQR determinant qnrB19 in serovars Havana, Give, Heidelberg, and Meleagridis in Mérida, Venezuela [10].
In this study, based on the patterns of resistance forlactam antibiotic and an unusual quinolone resistance phenotype with decreased susceptibility to ciprofloxacin (MIC: 0.5-1 g/mL) but still susceptible to nalidixic acid (MIC: 4-8 g/mL), we strongly suspect the presence of plasmids harboring ESBLs and qnr genes in two different Salmonella serovars from clinical sample of pediatric patients and raw chicken meat.
Amplification and sequencing of ESBLs and qnr genes revealed the presence of TEM-1 + SHV-12 in S. Give SG9611 and TEM-1 + CTX-M-2 in other three strains: S. Give SG9811, S. Heidelberg SH7511, and SH7911, associated in all cases with the qnrB19 gene as the only determinant responsible for the quinolone phenotype.
The mechanism of the Qnr protective effect is not completely understood [6]. However, it has been proposed that the Qnr proteins interact with DNA gyrase and topoisomerase IV, hindering the action of the quinolones and minimizing their inhibitory effect, while isolates with a qnr gene may be less likely to develop topoisomerase mutations than other strains [6][7][8]. The results of this study agree with these observations. The QRDS of the gyrase and topoisomerase IV genes were screened for the most commonly occurring mutations, but no resistance-associated substitutions were detected.
Although there are few studies reporting the prevalence of qnr genes in Salmonella isolates from South American countries, alleles belonging to the qnrB are the most frequently detected in the Enterobacteriaceae family [1,6], in accordance with the results obtained in this study.
Plasmids with qnr genes have been found to cotransfer TEM, SHV, and CTX-M genes. Particularly, the association between QnrB-like determinants and SHV-12, CTX-M-9, CTX-M-14, and CTX-M-15 has been reported in NTS strains [1,4,6,7]. In this study, qnrB19 and TEM-1 , SHV-12 , and/or CTX-M-2 genes located in plasmids were successfully transferred by transformation to susceptible E. coli recipient. Also, the resistance to gentamicin and tobramycin was cotransferred. It is recognized that insertion sequences (IS), as ISCR1 or ISEcp1 associated with qnrB genes, provide a putative promoter region for the expression of genes encoding resistance to aminoglycosides, trimethoprim, chloramphenicol, and -lactams [6,21]. Additionally, the coexistence between qnr and aminoglycosides was expected because qnrB genes are frequently located in integrons [6,9]. Regardless that all the four plasmids purified from transformant cells possessed TEM-1 , SHV-12 or CTX-M-2 , and qnrB19, only one of these (p9611trf) exhibited differences of size (∼13 kb) and a particular restriction pattern. It is possible that the organization of diverse genetic elements that participate in mobilization, transposition, recombination, and resolution, as well as deletion and insertion of DNA, generates variations in size and diversity of plasmid structures.
According to the chronology of isolation and origin of the strains studied here, we believe that the source of the qnr gene might not be directly related to the use of quinolone, since these drugs are not prescribed in pediatrics patient, but possibly a coselection of the qnr-harboring plasmids could be related to a linkage with other resistance genes carried on the same plasmids ( ESBLs ). Moreover, fluoroquinolones are widely used in veterinary medicine and poultry production, and qnr-positive NTS isolates could be selected and transmitted to humans through the food chain [22].
Based on several reports, the use of fluoroquinolones in infections caused by Salmonella isolates with reduced susceptibility may lead to treatment failures and promote the selection of bacteria with higher-level resistance to quinolone, thereby fostering the effect of others mechanisms of resistance as mutations [5,7,8,23].
The real prevalence of PMQR is usually underestimated as there are no reliable phenotypic methods to detect their presence [4]. Also, clinical isolates harboring PMQR determinants did not show any significant change in their MICs when compared with isolates susceptible to nalidixic acid and negative for presence of qnr genes. Hence, it is important to revise fluoroquinolones susceptibility testing practice, since strains with similar phenotype as those described in this study might be undetected in clinical laboratories using traditional phenotypic methods.
While prevalence data from an international survey that included 13 European countries identified the qnrS gene in a Salmonella sp. collection from human clinical sample and the qnrD gene in NTS strains of eight different serovars isolated from animal sources [24], and a Danish study reported the coexistence of CTX-M-15 and SHV-12 genes in a single plasmid associated with qnrB2 genes in S. Concord and S. Senftenberg isolated from human patients [1], our results showed the presence of qnrB19 associated with TEM-1 , SHV-12 , and CTX-M-2 in S. Give and S. Heidelberg from pediatric patients and raw chicken meat. Nevertheless, prevalence data should be interpreted with caution, since selection criteria for strains included in surveys could potentially bias the results because many studies have been performed with isolates collected over a short period of time, in the context of an outbreak or strains that are resistant to various antimicrobials agents [6,22].
Unfortunately, data on the occurrence of ESBL associated with PMQR in NTS strains from South America are scarce. To date, only in Brazil the presence of a qnrA1 in 100 isolates of S. Enteritidis and qnrB19 in one strain of S. Corvallis from poultry origin has been described [25].
Although this study includes few NTS isolates, we could prove the association of transferable quinolone resistance determinant qnrB19 with extended-spectrum -lactamases International Journal of Microbiology 5 in S. Give and S. Heidelberg in Venezuela. This represents a starting point for the development of surveillance programs aimed at the detection of ESBL and PMQR.

Conclusion
This study highlights the circulation of PMQR determinant qnrB19 associated with TEM-1 , SHV-12 , and CTX-M-2 in S. Give and S. Heidelberg from pediatric patients and raw chicken meat in Venezuela. The emergence of the combination of PMQR genes with ESBLs may contribute to the appearance of MDR Salmonella and jeopardize the usage of valuable antibiotics. We hope that recognition of factors associated with increasing resistance and the study of the molecular mechanisms involved can lead to a more focused use of antimicrobial agents which, in turn, will reduce the selection and spread of PMQR determinants and/or ESBLproducing enteropathogens.