Seed-Borne Fungi Associated with Diverse Rice Varieties Cultivated in the Western North Region of Ghana

Rice is a major staple in the Ghanaian diet. However, its production is constrained by fungal diseases. A survey was conducted in 2018 in three selected districts in the Western North Region of Ghana using a structured questionnaire and face-to-face interaction with 230 farmers to assess their knowledge, perceptions of seed-borne fungal diseases, and management practices. Additionally, fungi associated with farmer's seeds were isolated and identified through the Agar and Blotter tests. Findings indicate that 72.7% of the farmers in the selected districts relied on their saved seeds for planting. Thirteen fungal genera were associated with the rice seed samples collected from the three districts. The Juaboso district had the majority (13) of seed-borne fungi. The seed samples were categorized into various forms of discolouration, and significant differences (P < 0.05) existed among the seed samples for this parameter. The AGRA rice, a farmer-saved seed from Juaboso, had the highest level of seed discolouration (41.96%). Fungi identified to be associated with the dark brown/brown discolouration of rice seeds were Bipolaris spp., Fusarium spp., Macrophomina phaseolina and Aspergillus spp. The only fungus associated with the yellow/pale yellow colour was Bipolaris spp. The fungi Bipolaris spp., Curvularia spp., and Botryodiplodia spp. were associated with the dark spot discolouration. Alternaria spp., and Aspergillus spp. were observed on the greyish white seed discolouration sample. Fungi are associated with rice cultivation and vary according to district and rice variety. A complex of pathogenic and saprophytic fungi therefore infects rice grains both in field and storage conditions.


Introduction
Rice is an important food source for more than half of the world's population. About 480 million metric tons of milled rice are produced worldwide each year [1]. Consumption of rice is rising swiftly over any other produce in Africa because it is seen as a suitable food by the growing urban population [1]. Rice production area harvested and yield were 973,000 tonnes, 331,471 ha, and 29,354 hg/ha for the year 2020 [2]. Ghana spends about $450 million on rice imports to make up for the shortfall in supply [3]. Te husk of rice can be used to feed animals as well as used as a source of fuel and in biochar preparation. In developing countries, rice serves as the most important crop and plays a major role in poverty alleviation and food security attainment [4]. Rice is therefore loose smut, fag smut, Karnal bunt, and ear cockle of wheat [10].
Worldwide, about 56 fungal pathogens are reported to infest rice, of which 41 are reported to be seed-borne [11]. Seed-borne infections in rice cause 50-80% yield losses, depending on the agroecology, disease severity, and crop susceptibility [12]. In the Ashanti Region of Ghana, about 20 fungal species (16 pathogens and 4 saprophytes) associated with rice seeds have been identifed [13]. Te most common fungal species recognized included Bipolaris oryzae, Fusarium verticillioides, Fusarium oxysporum, Cercospora sp., and Curvularia lunata [13,14]. Farmers who rely on their farm-saved seeds are likely to contaminate their subsequent seeds for the next planting season in instances where there is seed-borne pathogen transmission from the soil.
Tis research was carried out to assess farmers' knowledge and their perceptions on fungal diseases associated with rice, and also isolate and identify fungal diseases associated with rice production in the Western North Region of Ghana.

Field Survey: Assessing Farmers Knowledge on the Fungal
Seed-Borne Diseases of Rice. A feld survey was conducted in the selected districts within the Western North Region, one of the newly established regions in Ghana. Te selected districts were Sefwi Waiwso Municipal, Juaboso District, and Bodi District. Te districts were selected based on previous records within the districts as hotspots for some seed-borne diseases, namely, "bakanae," "foot rot," and "blast [15]. Te selected communities were also based on their popularity for the production of rice within the districts. Tese  Between October and December, 2018, a feld survey was conducted using a structured questionnaire to gather information from the farmers (Supplementary Method). Farmers were selected randomly as respondents. Te information gathered included demographics about the farmers, sources of seeds, production, varieties, disease incidence, and control, as well as their knowledge on fungal seed-borne diseases. A printed picture plate of diseases of rice were used to aid the farmers identify and compare symptoms they observed on their various farms. A sample size of 230 farmers was selected at random for the survey (https://wacci.ug.edu.gh/content/francis-mensah-ackaah) [16]. Te respondents consisted of 86, 74, and 70 for Bodi district, Juaboso district, and Wiawso municipal, respectively.

Seed Sample Collection.
Twelve samples were collected, comprising farmer-saved seeds and certifed seeds, between November and December, 2018. Te samples comprised three certifed seed samples, eight (8) farmer-saved seed (FSS) samples, and one (1) local seed grower seed sample. Te samples were made up of the following varieties: AGRA rice, Bosome mmienu, Kotoko, Mercy, Lapete, and Agya Amoah rice (Table 1). Te farmer-saved seed samples of the AGRA rice were collected from three selected localities within a district and bulked as the farmers-saved seed sample from that particular district. Samples of seeds collected from the various districts were placed into zip-lock bags, labelled, sealed, and sent to West Africa Centre for Crop Improvement (WACCI) seed laboratory and the Plant Pathology Laboratory at the University of Ghana for seed health tests and pathogen isolation and identifcation, respectively.

Physical Quality
Assessment. Physical quality assessment was undertaken to separate the pure seeds from the other foreign materials, e.g., weed seeds, stones, etc. through physical examination. Samples were thoroughly mixed, and 500 g of each sample was weighed for the purity, test which was done with the use of a diaphanoscope (Ultra Swift Lite System, China). Te samples examined were categorized into broken/cracked, seeds with hull/husk, spotted seed/ discolored seed, insect damage, and undamaged. All individual components were weighed and expressed as a percentage by the weight of the whole component using the formula; (weight of component/total weight) * 100. Te discoloured seeds were grouped into various colours (dark spot, yellowish/pale yellow brown, and greyish white) using a colour chart.

Germination Test.
Te guidelines of the International Seed Testing Association (ISTA) were followed in the germination test. Te experiment involving the top of paper method (TP), involved 100 seeds (25 seeds per plate) replicated 3 times for each sample placed on Petri plates and laid with moist flter paper. Te petri dishes were covered with lids. Te set up was kept in a germination room under suitable environment (85-90% RH, 25°C). After 10 days, the seeds and seedlings were evaluated and categorized into normal seedlings, abnormal seedlings, hard seeds, fresh seeds, and dead seeds as a percentage of the seeds examined as classifed by ISTA. Seedlings that had well developed plant parts, including a well-developed rooting system and welldeveloped green leaves emerging, were classifed as normal seedlings. Seedlings that lacked one of the essential plant part such as a root or leaf or had other defects were classifed as abnormal seedlings. Freshly ungerminated seeds were classifed as hard seeds. Rotten seeds that failed to germinate were classifed as dead seeds.
Te germination and composition percentages were calculated based on the average of 100 seeds in 3 replicates and expressed as a percentage using the formula stated below. 2.6. Blotter Test. Hundred seeds each of the various samples were randomly selected and surface sterilized with 2% sodium hypochlorite (NaOCl 2 ). Filter papers were moistened and placed in petri plates. One hundred (100) seeds, in batches of 10 seeds, were arranged per plate and replicated 3 times. Te seeds in the plates were in incubated for 7-8 days at 25 + 2°C. After the incubation period, pathogens were morphologically identifed based on the colony's colour, shape, and size. A compound microscope (Ultra Swift Lite System, China) was then used to examine and confrm the pathogens on the basis of growth of mycelium and other fruiting bodies, spore shape and size as described in the technical bulletin on seed-borne diseases and seed health testing of rice [18]. Unknown cultures were grown on Agar media for improved growth to aid identifcation.
Percentage prevalence of individual fungi � Number of infected seeds by individual fungal X 100 Total number of seeds plated .
2.7. Agar Method. Potato Dextrose Agar (PDA) enhanced axenic fungal growth for further identifcations after usage of the incubation method. Five (5 g) powdered agar was mixed with 100 ml of water and stirred. It was then autoclaved for 15-20 minutes, after which it was cooled to about 50°C. Te Agar was then transferred into the petri-dishes, which solidifed in about 20 minutes. Antibiotics and amoxicillin, were added to the Agar to inhibit the growth of bacteria. After the solidifcation phase, the seeds were surface sterilized with sodium hypochlorite in order to eliminate any form of contamination as well as saprophytic organisms. Te saprophytes grow rapidly on the media, which mostly prevents the growth of important pathogens of interest. Te seeds were then plated and incubated for 7-8 days at 25 + 2°C. After the incubation period, pathogens were morphologically identifed based on the colony's color, shape, and size. A compound microscope (Ultra Swift Lite System, China) was then used to examine and confrm the pathogens on the basis of growth of mycelium and other fruiting bodies, spore shape and size as described in the technical bulletin on seed-borne diseases and seed health testing of rice [18].

Determination of Percentage Incidence of Fungi on
Samples Collected from the Tree Selected Districts. Te seeds infected by fungi were counted for each sample, divided by the total number of seeds plated, and multiplied by 100% to determine the percent incidence.
2.9. Fungi Associated with Discolored Seeds. Hundred (100) seeds each from the six varieties were sampled and critically examined. Te seeds were grouped according to their colours. Te discoloured seeds were grouped into diferent categories and plated using the blotter method. Te diferent categories were dark spot, brown/dark brown, yellow/pale yellow, and greyish white.

Data Analysis.
Te IBM SPSS STATICSTICS 22 was used to analyze the feld survey data. Fungal pathogen count data were subjected to analysis of variance (ANOVA), and the means were separated using the least signifcant difference (LSD) at a 5% probability level, in GenStat Statistical Package (12 th Edition).

Source of Seeds for
Planting. Forty-three percent (43%) of the respondents planted their saved seeds, 28.3% of the farmers planted certifed seeds obtained directly from various MoFA ofces (Crops Services Department), and 28.7% used both farmer-saved and certifed seeds.

Recognition of Diseases
3.2.1. Rice Blast. One hundred and seventy-nine (179) farmers, representing 77.8% of the respondents, reported that they have observed symptoms of blast at various stages of rice production. However, 51 farmers (22.2%) indicated they did not observe any symptoms of blast on their rice seeds.

Seedling Rot.
Seventy-three (73) farmers representing 31.7% of the farmers responded they observed symptoms of seedling rot, however, 157 farmers (68.3%) indicated they did not observe any seedling rot symptoms.

Seedling Blight.
Over seventy percent (73.5%) of the farmers had observed seedling blight before, as shown to them on a picture plate. About 26.5% responded that they had not observed any symptoms of seedling blight in their felds.

Respondents Perceptions of the Causes of Diseases.
About (25%) of farmers indicated pathogens solely caused diseases on their farms, and 24% indicated both pathogens and soilborne organisms as major causes of diseases. Twenty percent (20%) of the respondents reported soil-borne organisms caused diseases in their farms. However, 9% did not know the causes of diseases in their felds, and 6% reported seed-borne diseases were the causes of diseases on their farms.

Assessment of Physical Quality of Seeds.
All seed samples examined had some percentages of undamaged seeds, cracked or broken seeds, and discoloured seeds (Table 3). Tere were signifcant diferences (P < 0.05) among undamaged seeds. Te least undamaged seeds (93.1%) were the "Bossome mmienu" seeds. Te AGRA FSS rice seed sample collected from Juaboso had the least undamaged (50.21%) seeds. Seed discolouration was observed on seed samples obtained from farmers as they saved seeds. Tere were signifcant diferences (P < 0.05) among the seed samples regarding seed discolouration. Te AGRA rice sample collected as Farmer-saved seed from Juaboso, had the highest level of seed discoulouration (41.96%). Other seed varieties "Lapete" and "Mercy" had seed discolouration values (39.73% and 38%), respectively. Tere were signifcant diferences (P < 0.05) among the seed samples for broken/ cracked seeds. Te seed sample obtained from MoFA ofce, Juaboso and NRGP had the least percentage (2.81% and 2.91%) of broken/cracked seeds respectively. Te highest percentages (7.91% and 7.83%) of broken seeds were on the "Mercy" rice seed sample and the AGRA rice variety, respectively.  (Table 4). Te sample with the lowest germination rate was the AGRA rice variety collected from farmers in the Bodi district.
Comparing the AGRA rice varieties, the samples taken from MoFA ofces that were certifed seeds performed better in terms of germination than the farmer saved seeds. Tere were signifcant diferences among them. AGRA rice sample collected from Wiawso scored highest among the AGRA rice samples with 93.33% normal seedlings followed by the AGRA rice variety collected from farmers in the Wiawso Municipality (89.67%). Te AGRA rice sample collected from farmers in the Wiawso municipal was the only FSS sample which performed better than the MoFA sourced samples. Te AGRA rice seed sample collected from farmers in the Bodi district performed worst in terms of germination, scoring 62.33% of normal seedling germination. Tere were signifcant (P < 0.05) diferences among the abnormal seed percentages of the various samples. Te AGRA sample collected from the Wiawso Farmers and the Mercy rice seed sample collected from the Juaboso had the lowest percentages (4%) of abnormal seedlings.

Fungi Associated with the Rice Seed Samples
Collected from the Tree Selected Districts. All seed samples collected from the three selected districts had various seed-borne fungi at diferent levels of infection (Table 6). Tirteen fungi in total were associated with the rice seed samples collected. Six out of the thirteen fungi identifed were saprophytic, and seven were pathogenic. Some the saprophytes were Aspergillus favus, Asperfllus niger, Mucor spp., Penicillium spp., and Rhizopus spp. All the seed samples collected scored high levels of saprophytes. Not all the fungi identifed were present on all the seed samples collected from the diferent districts. Te Juaboso district scored the highest number of fungi identifed (Table 6) whiles the Bodi district scored the lowest number of fungi associated with rice seeds. In the Bodi district, Aspergillus niger scored the highest frequency of fungi infection (28.25%) followed by Macrophomina spp. (23.65%) while Aspergillus favus scored the least frequency of infection with 5.95%.
In the Juaboso District, Aspergillus favus scored the highest frequency of fungi infection at 19.42%, followed by Aspergillus niger, 12.38%. Collectotricum spp. scored the least frequency of infection at 1.92%. Aspergillus favus scored the highest frequency of infection in the Wiawso District, followed by Curvularia spp. (Table 6).

Frequency of Fungi Associated with the Diferent Rice
Seed Varieties Sampled in the Selected Districts. Te variety AGRA scored the highest prevalence of fungi followed by the variety "Bossomme mmienu" (Table 7). Te variety Mercy scored the least number of fungi infections with only fve fungi reported to be associated with the Mercy rice seed sample. All thirteen fungi identifed were reported to be associated with the AGRA rice seed variety. Aspergillus favus, Aspergillus Niger, Rhizopus spp., and Penicillium spp. were associated with all the rice seed varieties sampled. Te "Mercy" rice variety had the highest prevalence of Aspergillus niger, whiles "Agyamoah" rice variety had the highest prevalence rate of Aspergillus favus. Bipolaris oryzae was observed on only three varieties: AGRA, "Agyamoah," and "Bossomme mmienu," with 13.77%, 9.59%, and 7.36%, respectively. Te "Agyamoah" rice variety had the highest  (Table 7).

Frequency of Fungi Infection of the Variety AGRA in
the Selected Districts. Eleven (11) out of the thirteen fungi identifed were observed on the AGRA rice seeds. Te Bodi farmer-saved seed sample had the highest number of fungi associated with the seeds however the AGRA Farmer Saved

Discolored Seeds and Associated Fungi.
Fungi cause seed discolouration in varying forms. Fungi which had dark brown/brown discolouration of rice seeds were Bipolaris oryzae, Fusarium spp., Macrophomina spp. and Aspergillus spp. (Table 9). However, only Bipolaris oryzae was associated with the yellow/pale yellow colour. Bipolaris oryzae, Curvularia spp., Fusarium spp., and Botryodiplodia spp., were associated with the dark spot discolouration. Alternaria spp. and Aspergillus spp. were identifed on the greyish white seed discolouration sample.

Knowledge of Farmers on Varieties of Seeds Cultivated.
Majority of the farmers interviewed planted Agra rice followed by Lapete, a local rice variety. "Bossom mmienu" and "Kotoko" varieties were cultivated by only 0.4% of the respondent. AGRA rice was the mostly used variety by the farmers constituting 83%. Tis is as a result of various Programs and projects enrolled by the Government of Ghana through the Ministry of Food and Agriculture and other institutions such as Ghana Rice Interprofessional Body. In a previous study by Ragasa et al. [19], increased use of certifed seeds was encouraged through projects initiated by the Government of Ghana and or Development Agencies. Te Planting for Food and Jobs under MOFA was a major fagship program introduced by the Government of Ghana in 2017 to promote the use of the AGRA rice variety. In spite of all these interventions, some farmers still preferred to use local farmer-saved seeds. A rice variety "Agya Amoah" which was introduced to the farmers in the selected districts back in 1987 was still in use by some farmers. Te farmers indicated that, even though there were programs and projects to enhance the use of certifed seeds and improved varieties, the local varieties, for example, Lapete was also an early maturing variety and resistant to pest and diseases. Tey explained that the local varieties were able to withstand harsh weather conditions such as drought, pests and fooding compared to the newly improved varieties, even though the improved varieties give higher yields. In a study by Marfo et al. [20] rice varieties such as "AgyaAmoah," "Mercy," "Bosome Mmiensa," "Bosome Nnan," "Kotoko," "Abankora," and "Sikamo" were grown by farmers in the Western Region. However, the preferred variety was "Agya Amoah" because of the following characteristics: yield (45%), grain size (17%), and early maturity (16%).

Source of Seeds for
Planting. Majority of the farmers interviewed (70%) use their saved seeds for planting in the next season, and about 28% of them used certifed seeds. Te farmers used their own saved seeds because, they did not have access to certifed seeds easily, some also preferred to use their saved seeds because of lower costs. Some farmers however indicated there were no diferences between their saved seeds and the certifed seeds. Tose who used certifed seeds obtained their seeds from the district agriculture offces, few agro input shops, and NGOs. Te percentage of farmers (28%) using certifed seeds relatively increased compared to percent of farmers listed in the literature study (11%) [19]. Tis was because certifed seeds were obtained from MoFA under the Planting for Food and Jobs (PFJ). Farmer-saved seeds were the main source of seeds for the farmers in the three districts. Over 70% of rice farmers depend on their saved seeds for subsequent seasons, especially in the sub-Saharan Africa where most farmers do not buy certifed seeds, because of high prices [21]. Tose who used farmer-saved seeds and local varieties acquired their seeds in the form of seed exchange, gifts, and from the local market.

Rice Seed Storage and Storage Practices of Farmers in the
Tree Selected Districts. Majority of the farmers (96.1%) in the three districts stored their seeds at home under ambient temperatures in nylon bags and jute sacks. About 2.6% of the   � not present, + � incidence below 10%, ++ � incidence between 11-30%, +++ � incidence above 30%. NB: + � low prevalence, ++ � moderate prevalence, +++ � high prevalence. 8 International Journal of Microbiology Table  9: Percentage of seed-borne fungi associated with various discolouration types in the rice seed samples collected from the three selected districts.
Aspergillus spp. farmers stored their seeds in a warehouse operated either by a private individual or MoFA, and 1.3% of the respondents stored their seeds in cold storage. Most farmers in developing countries, e.g., Ghana, store their seeds under ambient conditions where temperature and moisture content vary. Te 1.3% of farmers who stored their seeds in cold storage explained they were advised by some agricultural ofcers to do so. Tey usually stored seeds of new varieties introduced to them by agricultural ofcers. Tey stored their seeds in their home refrigerators usually only for a season at temperatures below 10°C. Te warehouse storage was managed by a private individual who stored assorted products for a fee payable monthly. Tey were usually rice mill operators who stored farmers' seeds for them. Te majority of farmers who stored their seeds in nylon and jute sacks followed the traditional practice of storing produce. Tey stored them either in the store rooms or kitchens without measuring temperature and relative humidity (RH) of the storage environments. Te practice of seed storage in these locations may afect the seed quality and increase the development of pathogens on the rice seeds. Te bags for storing seeds may not be properly sealed allowing seeds to be exposed to moisture and other environmental factors, which enhance growth of microorganisms on the seeds. Rice with a high moisture content >21% increases rice discoloration from temperatures 10°C to 40°C as storage period progresses. Tere is a higher expression of discoloration patterns on rice seeds stored at the high temperatures for e.g., 40°C compared to storage at lower temperatures [22]. Most rice farmers in Ghana lack moisture meters to determine the moisture content of their seeds and this together with storage environments with high temperatures >10°C results in mold development and discoloration of their seeds. In the study by [22] the total discoloured kernel area increased with increasing storage moisture content (MC), temperature, and storage duration, with about 100% discolouration observed at 16 weeks at 40°C storage temperature.

Dark brown/brown
Over 98% of the farmers did not treat their seeds (farmer-saved or certifed) before storage. Farmers who did not treat their seeds said, thorough drying under the sun enhanced the prolonged storage of their seeds. Tose who treated their seeds before storage used various insecticides such as Akate Master and Lambda. Tese insecticides were used because they believed any poisonous chemical will be efective in controlling any kind of infection or pathogen. Te chemicals used by the farmers for treating their seeds were also used on other crops, for e.g., cocoa. Rice seed coating with for example Trichoderma spp., has been found to be very useful in maintaining the seed quality and tolerance to biotic and abiotic stress [23].
Most of the farmers did not really observe changes associated with their seeds during storage. Tey did not bother to check for these changes until planting. However, 61% of farmers in the Ashanti region of Ghana observed changes associated with rice seeds during storage, they usually observed pest infestation during storage as reported [13].
Most of the farmers stored their seeds for three to fve months. Only few of them stored up to 12 months. Farmers in the three districts did not store their seeds beyond one year. Inadequate storage facility inhibited storage for relatively longer periods. Most of farmers did all year-round farming (two to four times a year), therefore will need seeds for planting frequently. In the Ashanti region, 48% of rice farmers stored their seeds beyond six months [13]. Tose who stored their seeds for longer periods were likely to attract more pathogens. Depending on the storage condition, there quality of seeds reduced with increasing storage duration [24].

Farmers Perception of Occurrence of Some Important Rice
Diseases. Farmers had little knowledge about seed-borne diseases during a focal group discussion session. An average of about 60% of the farmers reported to have observed various symptoms of the seed-borne diseases. Te majority of the farmers (77.8%) observed symptoms of blast at various stages; 73.5% observed seedling blight whiles only 30% observed seedling rot. In a previous study, the majority of farmers interviewed in the Greater Mwea region of Kenya were aware of the occurrence of rice diseases for e.g., rice blast disease [25]. From the survey conducted, farmers ignored symptoms of the various seed-borne diseases when these appeared during storage or on the felds. Tose who observed the symptoms of diseases perceived various causes of the diseases. About 50% of the farmer-perceived diseases were caused by pathogens, and few of them attributed the causes of the diseases to the source of seeds, soil, and insects. Farmers in some selected districts in Ghana paid much attention to the symptoms of diseases. Te farmers made observations of symptoms of seed-borne diseases such as rice blast, and they associated the symptoms with being caused by pathogens [15]. Similarly, rice farmers in Burkina reported diseases as a major constraint to rice production and were aware of the causes of the diseases [26].

Knowledge of Seed Borne Diseases.
Majority of the respondents (87%) had observed the incidence of seed-borne diseases. Tey responded that they were aware of the fact that diseases "sit in the seeds." Tirteen (13%) of the respondents did not have any knowledge on seed-borne diseases. In one study by Kihoro et al. [25] in Kenya, they reported that 98% of rice farmers had knowledge about rice blast, a seed-borne disease.
Out of those who had knowledge about seed-borne diseases, 50% treated their seeds with diferent kinds of chemicals before planting. Tose who treated their seeds used any chemical they perceived to be poisonous for e.g., Akate Master, Lamda, and Dusband. Tose who did not treat their seeds before planting relied on the sun as a treatment to control seed-borne diseases [13]. Rice farmers in Bangladesh, treated their seeds with hot water, as well as selected fungicides (Bavistin 50 WP, Dithane M 45 and neem extract) [27].

Detection and Identifcation of Seed-Borne Fungi on the Rice Samples Collected from the Tree Selected Districts.
Te current study identifed thirteen fungi to be associated with the rice seeds (Table 5). In a previous study, rice seeds were associated with 20 fungi in the Ashanti Region. Among these, the following were not identifed in the current study, there were Alternaria padwickii, Alternaria spp.,, Cercospora spp., Curvularia lunata, Curvularia pallescens, Fusarium verticillioides, F. oxysporum, F. pallidoroseum, F. solani, Nigrospora oryzae, Phoma spp., Sarocladium oryzae, and Stemphylium spp. Trichoderma spp., Macrophomina phaseolina, Botrydiplodia spp., and Pyricularia spp. identifed in our isolations were absent in those of Asamoah [13]. Similar fungi as identifed in the current study have been identifed on rice in Ghana [15,28,29]. Mucor spp. identifed in this study, was recently isolated from rice seeds in Kenya [27]. In another study, Nustugah et al. [15] identifed Bipolaris oryzae, Pyricularia spp. and Magnaporthe spp. to be present in Ghana in the northern region and upper east regions of Ghana. Te absence of Magnaporthe oryzae from our study sites, confrms the absence of rice blast disease caused by these fungi in farmers' felds in the current study. Rice seedborne fungi such as Bipolaris oryzae, Cercospora sp., Colletotrichum sp., Curvularia lunata, and Curvularia pallescens have been reported [30,31]. Te high prevalence of storage fungi such as Aspergillus favus and Aspergillus niger on all rice seed samples was an indication that the seeds were stored under high temperature, and had high moisture content. High temperature and moisture at storage have been found to decrease germination of seeds of soy and maize as well as increasing the infection of Aspergillus favus. Te high prevalence of storage fungi such as Aspergillus spp. have been reported to be associated with high moisture content [32]. Te high prevalence of storage fungi may also cause seed rotting. Storage fungi such as Aspergillus spp. and Fusarium spp. not only enhance discolouration of the rice kennel but also secrete deadly mycotoxins detrimental to the human and animal health.
In a previous study, the fungi species Alternaria spp., Bipolaris spp., and Fusarium spp. were associated with various discolouration on rice [33]. In their isolations, Alternaria padwickii was responsible for the ash gray discolouration, Fusarium spp. was responsible for light pink and pale yellow discolouration. In the current study, Alternaria spp. and Fusarium spp. were responsible for grayish white discolouration. Bipolaris oryzae was associated with dark brown, brown spot, and brown discolouration.
Bipolaris spp. has been found to be the most important pathogen of rice and the most frequently identifed pathogen on rice seeds [27]. Tis organism has been reported to be the cause of brown spot and seedling blight diseases of rice. Pyricularia spp. has also been reported to be one of the principal causal organisms in rice blast. Both of these two important seed-borne fungi (pathogens) were identifed in the current study.

Conclusions
Tis study showed that seed-borne fungi were associated with all the rice cultivars sampled from the study area. Te fungi were made up of six saprophytes and seven pathogens. Tere was high prevalence of storage fungi associated with the rice seeds partly because of the storage practices employed by the farmers. Tere is therefore the need to elucidate the biochemical basis of rice discoloration and the relationship with specifc microorganisms. Tis will enable development of microbe-specifc interventions against rice discolouration.