Development of Pillared Clays forWet Hydrogen Peroxide Oxidation of Phenol and Its Application in the Posttreatment of CoffeeWastewater

This paper focuses on the use of pillared clays as catalysts for the Fenton-like advanced oxidation, specifically wet hydrogen peroxide catalytic oxidation (WHPCO). This paper discusses the limitations on the application of a homogeneous Fenton system, development of solid catalysts for the oxidation of phenol, advances in the synthesis of pillared clays, and their potential application as catalysts for phenol oxidation. Finally, it analyzes the use of pillared clays as heterogeneous Fenton-like catalysts for a real wastewater treatment, emphasizing the oxidation of phenolic compounds present in coffee wastewater. Typically, the wet hydrogen peroxide catalytic oxidation in a real effluent system is used as pretreatment, prior to biological treatment. In the specific case of coffee wet processing wastewater, catalytic oxidation with pillared bentonite with Al-Fe is performed to supplement the biological treatment, that is, as a posttreatment system. According to the results of catalytic activity of pillared bentonite with Al-Fe for oxidation of coffee processing wastewater (56% phenolic compounds conversion, 40% selectivity towards CO2, and high stability of active phase), catalytic wet hydrogen peroxide oxidation emerges as a viable alternative for management of this type of effluent.


Development of Heterogeneous Fenton-Like Catalysts.
One of the most effective technologies to remove organic pollutants from aqueous solutions is the Fenton's reagent treatment [1].Fenton's reagent was developed in the 1890s by Henry John Horstman Fenton and consists of a homogeneous solution of hydrogen peroxide and an iron salt [2].The mechanism for producing free hydroxyl radicals in Fenton (Fe 2+ /H 2 O 2 ) and Fenton-like processes (Fe 3+ /H 2 O 2 ) is very complex and thought to occur in the following stages [3][4][5][6][7]: Fe-OOH +2 −→ Fe 2+ + HO The success of Fenton processes for the oxidation of a variety of organic contaminants is attributed to the generation of hydroxyl radicals formed during the catalytic decomposition of hydrogen peroxide in acidic media, according to the above scheme [8][9][10][11][12][13].This reaction is propagated by ferrous ion regeneration, which mainly occurs due to the reduction of the ferric species produced by hydrogen peroxide.However, in these reactions, the rate constant of (1) varies between 63 and 76 L/mol•s whereas the rate constant of ( 5) is in the order of 0.01-0.02L/mol•s [13][14][15].This indicates that the consumption of ferrous ions is faster than regeneration.Therefore, during the process, the formation of a large amount of ferric hydroxide sludge occurs, which supposes additional separation [13,16].Both radicals HO • and HO • 2 react indiscriminately with organic matter, being the second less reactive.In a Fenton oxidation, the hydroxyl radical reacts rapidly with most organic compounds by abstraction of carbon bound hydrogen and double bound addition, initiating a sequence of oxidative degradation reaction which may lead to mineralization of CO 2 , H 2 O, and the production of carboxylic acids [6,[17][18][19].Catalytic oxidation reactions are almost exclusively limited to transition elements because these may exist in more than one state of oxidation, making the establishment of a repetitive oxidation-reduction cycle possible [20][21][22].Therefore, these reactions also occur with transition metal ions such as Fe 3+ or Cu 2+ and are known as Fenton-type reactions [23] as follows The application of a homogeneous Fenton system has as a disadvantage of the separation and reuse of the catalyst (Fe ions in solution).It is also necessary to control the pH solution so to avoid precipitation of iron hydroxide (pH > 5) [23].To resolve some of the above drawbacks, a heterogeneous Fenton-like catalyst has been developed, that is, solids containing the cations of transition metals [24,25].This way, metals and the selected support directly affect the catalytic activity because they are determinant factors in the stability of the supported metal and its dispersion [26,27].

Heterogeneous Fenton-Like Catalysts for Phenol
Phenol is also relevant in the field of environmental research because it has been chosen frequently as a model pollutant.Much data is available on its removal and destruction, in particular with respect to wastewater treatments [75].The presence of phenol in the environment is acutely toxic to mankind; hence, treatment of phenolic pollutants is essential before disposal [76].
Widely studied methods for the removal of phenol include biological treatment [77], extraction [78], and wet oxidation [79].However, advanced oxidation processes (AOPs) such as Fenton, photo-Fenton [80], ozone oxidation [81], and photo-catalytic oxidation [82] are successfully used for the removal of phenol [75].There is an increasing interest in the use of wet hydrogen peroxide catalytic oxidation (WHPCO) for the disposal of phenolic compounds in water, because this method (in comparison, for example, with wet air oxidation) avoids the use of costly reactors, it can be selective towards the conversion of specific substrates, and it is easy to manage [69,83].
One of the most popular catalysts for the oxidation of phenol with H 2 O 2 is zeolite Fe-ZSM-5, which has been found to be very active but shows diffusion limitations due to the relatively small sized pores [97].Transition metals containing mesoporous materials, such as MCM-41 and SBA-15, have been appropriate for the oxidation of phenols due to a larger pore size that is expected to enhance diffusion of reagents in comparison with microporous materials.However, these materials are not stable in the reaction medium and go through destruction of mesoporosity and high leaching of the active phase [75].
In the search for other iron containing heterogeneous Fenton-type catalysts that have a low leaching of the active phase at pH 3-4, where the phenol oxidation with H 2 O 2 is maximal [75], a new class of catalysts with a bidimensional open structure called pillared interlayered clays (PILCs) was developed.PILCs result from the intercalation of inorganic polycations into the interlayer space of clays to form rigid cross-linked material of uniform microporosity [98].Due to a high surface area and permanent porosity, they are very attractive solids for use in catalysis [99].The use of pillared clays as catalytically active supports for phases (mainly Fe and Cu) has the advantage that these transition metals show low leaching into a WHPCO reaction [46,56,58,60,100].

Developments in the Synthesis of Pillared Clays.
Barrer and MacLeod first introduced the concept of transforming a lamellar solid into a porous structure by inserting laterally spaced molecular props between the layers of a smectite clay mineral [101].A pillaring agent is any compound which can be intercalated between adjacent layers of a layered compound, maintaining the spacing between adjacent layers upon removal of the solvent, and inducing an experimentally observable pore structure between the layers [102].When montmorillonites pillared with hydroxylaluminium oligomer [Al 13 O 4 (OH) 24 (H 2 O) 12 ] 7+ , are heated in air at 500 • C, the Keggin ions lose their water ligands forming shorter Al 13 blocks (0.84 nm in height) that become the structure supporting pillars [103,104].Although the pillaring cation is the main Al 13 , the addition of a second inorganic cation can improve the thermal, adsorptive and catalytic properties of the pillared clays [99].
The classical pillaring method involves two steps: first, the addition of the precursor polymer solution (pillaring agent) into the diluted clay mineral dispersion (intercalation).A second step is the thermal treatment of the intercalated clay mineral [99].A wide variety of factors can influence the intercalation/pillaring process.This variation makes it difficult to compare the results obtained by different authors, and also good reproducibility becomes hard to achieve.The factors influencing this process include the nature of the raw clay used as parent material, nature of the metallic cations, the hydrolysis conditions (concentration, time, and ageing), intercalation time, temperature of synthesis and, finally, the washing, drying and calcination processes [105].
Variants of the traditional method have been researched in order to decrease the water volume and timing of the synthesis.Thus, it has become possible to decrease the volume of water by the use of concentrated suspensions, both clay and the pillaring agent [106][107][108][109][110][111].Also, the time of intercalation of montmorillonite with chlorhydrol has been reduced through the use of ultrasound, having led to the obtaining of Al-PILC with a high thermal stability and textural properties superior to those of clay modified with an exchange time of 24 h.In overall, during the intercalation, treatment with ultrasound [51,112,113] and microwaves [107,[114][115][116] has been proved to be an adequate method for reducing the contact time between clay and the pillaring agent [57].
Although, in the past 30 years, several studies highlighting some applications of industrial pillared clays have been published, such materials have not been used as commercial catalysts primarily due to the difficulty of extending the laboratory synthesis to an industrial scale [57].However, in 2005, a procedure for pillaring clays with aluminum, which minimizes the processing time and the amount of water used, allowing its extension to an industrial level was developed.To simplify the synthesis of Al-PILC, nonpurified clay and the pillaring agent in a solid state are used, so that clay powder and solid Al 13 nitrate are placed together in a dialysis membrane [117].
A diversity of raw clays has been used for the preparation of these pillared materials, around 80% corresponding to bentonite-montmorillonite [118].In pursuing the use and valorization of Colombian clay minerals, a series of systematic studies were carried out modifying, via pillaring of bentonite, clays from Valle del Cauca.One of the largest companies in Colombia exploiting and marketing bentonite is located in that Colombian region.The purpose of using such clays was to obtain catalysts (pillared bentonite) for removal of phenolic compounds in an aqueous solution by a heterogeneous Fenton system, in which the iron and/or cerium species were supported on bentonite.Table 1 summarizes results of the research conducted.
The first pillared bentonites with Al-Fe and Al-Ce-Fe were synthesized using the conventional method of diluted pillaring solution and diluted suspension of clay (2 wt%), which involves the use of large volumes of water and long synthesis times.The pillared clays were very efficient catalysts in the reaction of a phenol oxidation in diluted aqueous media under mild experimental conditions (25 • C and atmospheric pressure), as well as in the elimination of different intermediary compounds of the reaction, reaching highmineralization levels.The catalysts showed high stability in the reaction media due to the strong interaction between the iron species and the catalyst support.The incorporation of cerium showed a favorable effect in pillaring of the materials, allowing the increase of the basal spacing and enhancing the catalytic activity of the catalysts [47][48][49][50].Carriazo et al. performed the characterization of pillaring species of Al-Ce-Fe, finding three different crystalline structures (boehmite, α-Fe 2 O 3 , and CeO 2 ) into the solid synthesized from the Al-Ce-Fe polyhydroxocationic solution.The EPR analysis confirmed the formation of iron oxide particles and the likely inclusion of isolated Fe 3+ species into the alumina matrix [50].
To expand the pillaring process to an industrial scale, it was necessary to simplify procedures and optimization of the unit operations involved, particularly to decrease the volume of water and synthesis times.In this regard, Pérez et al. used ultrasound for aging and intercalating of pillared bentonite with Al-Ce and Al-Ce-Fe.The use of ultrasound showed a clear effect in the synthesis of this type of solids allowing the synthesis in a shorter time and preserving the physicalchemical characteristics as well as catalytic activity in the oxidation reactions [51].Olaya et al. used microwaves and ultrasound, both in the formation of the polymeric precursor and in the stage of intercalation in concentrated suspensions of clay, finding that this type of radiation improves the formation of pillars and promotes the catalytic activity of the final solid [53][54][55].
With a procedure similar to that developed by Aouad et al. [117], Sanabria et al. propose a new methodology for the synthesis of clays pillared with mixed Al-Fe and Al-Ce-Fe systems, using minimal volumes of water and reducing intercalation times.This methodology involves the synthesis of the solid polymeric precursor and its use as a pillaring agent.The use of powdered clay and a solid pillaring agent, contained in a dialysis membrane, considerably reduces the amount of water used in the conventional synthesis.The intercalation of the polymeric precursor in clay is favored by the use of ultrasound, reducing long contact times required in conventional synthesis into diluted suspensions [52,56,57].The synthesis of pillared clays by mixed systems Al-Fe and Al-Ce-Fe in a concentrated medium allows a 90%-95% decrease in the volume of water and a reduction in the intercalation times between 70% and 93% with respect to the conventional synthesis.The pillared clays using this new methodology show a catalytic activity and selectivity comparable to those of solids synthesized by the conventional method in a dilute medium [56,119].An important aspect is that the Fe introduced into the clays is preferentially associated with the alumina pillars and not with surface Fe oxides (cluster).This is explained by the high stability of the active phase in the reaction medium [57].
The commercial use of PILCs based catalysts not only depends on the optimization of the synthesis, but also on the ability to shape the powder material into pellets, agglomerates, and so forth.These materials should keep their chemical properties, reactivity, and stability in the reaction medium.The manufacture of pellets, Raschig rings and monoliths, that involve pillared montmorillonites has basically been accomplished by the use of extrusion techniques [120].
With the developments in the synthesis of pillared clays in a concentrated medium, the manufacture of extruded materials with Al-Fe Al-Ce-Fe pillared bentonites was achieved.We found that the most adequate composition of the mixture of poly(hydroxo metal) bentonite/binder/water for the manufacture of extrudates with B-AlFe and B-AlCeFe was 42/28/30.The use of poly(hydroxo metal) bentonite (dried at 60 • C) and not the pillared bentonite (after calcination at 400 • C) considerably improved the mechanical stability of the extrudates.The mechanical resistance of B-AlFe and B-AlCeFe based extrudates depended on the calcination temperature.At 500 • C, good mechanical strength and sufficient stability to immersion in water were achieved.The extrudates largely preserved the structural and textural properties of Al-Fe and Al-Ce-Fe-PILC and retained the catalytic properties of powdered pillared clays [27,61].

Wet Hydrogen Peroxide Catalytic Oxidation (WHCPO)
of Phenol Using Pillared Clay.Advanced oxidation processes (AOPs) are based on physical and chemical processes capable of fundamental changes in the chemical structure of contaminants, as they involve the generation and use of transitory species with a high oxidation power, mainly the hydroxyl radical (OH • ) [6].Among advanced oxidation processes, the activation of hydrogen peroxide by means of a solid catalyst (wet hydrogen peroxide catalytic oxidation, WHPCO) has turned out to be the most promising process for the oxidation of phenol and phenol-like compounds and application in the treatment of wastewater [23,83,118].
Oxidation processes using hydrogen peroxide as oxidant have turned out to be a viable alternative for the wastewater treatments of medium-high total organic carbon concentrations.Furthermore, iron is an abundant and nontoxic element; hydrogen peroxide does not give origin to any harmful by-products and it is a nontoxic and ecological reactant [6,121].
Among the different materials used as support for oxidation reaction in a liquid phase, pillared clays represent around 7% in the existing literature.However, the interest for pillared clays has increased substantially in the last decade, given their use in different oxidation processes such as WHPCO and photocatalytic oxidation [118].The properties of pillared clays-based catalysts have been mainly studied in the wet hydrogen peroxide oxidation of phenolic compounds as model molecules, among them are phenol [44,45,48,57,58,62,65,68], 4-nitrophenol [122,123], tyrosol [70], and p-coumaric and p-hydroxybenzoic acids [69].
Pillared clays with Al-Fe and Al-Cu are promissory catalysts for wet hydrogen peroxide oxidation of phenol, because they combine a porous support and active sites for the adsorption of organic compounds in the activation of H 2 O 2 [68].The use of pillared clays with copper in a Fenton reaction would be important since the optimal pH of that reaction is close to 6, while for AlFe-PILC, the optimum pH ranges between 3.5 and 4.0 [46,62,100].The properties of AlCu-PILC were compared with those of analogous iron-based clays (AlFe-PILC) in the wet hydrogen peroxide catalytic oxidation of model phenolic compounds.These two catalysts have comparable performances in all these reactions, although they showed some differences in the rates of the various steps of the reaction.In particular, AlCu-PILC showed a lower formation of oxalic acid (main intermediate reaction) with respect to Fe-PILC [69].Among the metals studied as active phase in oxidation processes with pillared clays-supported catalysts, iron is the most frequently used metal, followed by titanium, copper, and chromium [118]; hence, some of the most studied are the pillared clays with Al-Fe.
In general, pillared clays with Al-Fe are efficient in phenol elimination under mild experimental conditions (atmospheric pressure and room temperature) without considerable leaching of metal ions [44,46,56,60].AlFe-PILC achieved high conversions of phenol and TOC thus showing to be very selective towards the formation of CO 2 , as shown in Table 2.
Cerium (Ce) was introduced into pillared clays with Al and Al-Fe, improving the metallic dispersion properties, increasing the pillars resistance [124][125][126], and favoring the redox properties of the active metallic phase [48,127].The catalysts obtained by the pillaring of Colombian bentonite with Al-Fe or Al-Ce-Fe are highly efficient for the reaction of phenol oxidation in diluted aqueous media in moderate conditions of temperature and pressure (25 • C and atmospheric pressure).The low iron leaching indicate that the active phase of these catalysts is strongly fixed to the clay support and pillars, and that it is highly stable under oxidizing conditions of the reaction medium [47,48].
Most research on the use of pillared clays for the oxidation of phenol was conducted with the powdered catalyst and a few with pellets of Al-Fe pillared clay [27,61,[66][67][68].Therefore, the development of catalysts based on pillared clays not only depends on the optimization of the synthesis, but also on the ability to shape the powder material into structured shapes (e.g., pellets, Raschig rings, and monoliths) that retain the chemical properties and reactivity of the active phase and can be used in fixed bed reactors [27].

International Journal of Photoenergy
B-AlCeFe As above As above As above [51] AlCeFe2424 Fe/Al-PILC T = 50 • C Phenol 100 mL, 250 mg/L Catalyst = 0.05-0.1 g, pH = 5.0-5.5, time = 3 h B-Cu [10] As above pH = 5.0-5.5 X phenol = 37%-70% X TOC = 96%-100% Cu leaching < 0.4 ppm X phenol = 100% X TOC = 65% Fe leaching < 2.0 ppm [45] International Journal of Photoenergy 7  Extrudates of an Al-Fe pillared clay catalyst suitable for packed-bed operations are evaluated for oxidation of phenol using hydrogen peroxide as the oxidant.The reaction was processed in a semibatch basket reactor under rather mild conditions.Operational parameters were studied under the following conditions: temperature from 25 to 90 • C, atmospheric pressure, initial phenol concentration from 100 to 2000 ppm of the liquid phase, catalyst loading from 0 to 10 g/L, and input H 2 O 2 concentration from 0.15 to 0.6 mol/L.Under these conditions, the Al-Fe pillared clay catalyst achieves a total elimination of phenol and significant total organic carbon (TOC) removal.This catalyst can be used several times without any change in its catalytic properties, and, hence, it would be a promising catalyst for industrial wastewater treatment [66].
Extrudates manufactured with pillared bentonites were also employed in phenol oxidation at 25 • C and atmospheric pressure.Extrudates with B-AlFe and B-AlCeFe reached 100% conversion of phenol and TOC conversion between 30% and 62%, after 9 h of reaction.Once the reaction was completed (9 h), the catalyst was removed and the leachate was recovered and analyzed, showing Fe values in the range of 0.11-0.14ppm.The outstanding differences in time for the phenol conversion and TOC of extruded materials compared to the powdered materials were a consequence of the agglomeration process and the inherent diffusional limitations [61].The extruded catalyst retained its catalytic activity during at least 10 consecutive tests (80 h), and only a loss of mechanical stability was observed for the series containing B-AlFe as the active phase.This result proved the importance of cerium in the synthesis of pillared clays with mixed systems because it not only increased the catalytic activity due to its oxygen storage capacity, but also increased the mechanical stability of extruded catalysts in the reaction medium [27].

Fenton Oxidation with Pillared Clays for Wastewater Treatment
The interest for pillared clays has dramatically increased in the last years, in great part due to their potential applications as catalysts.Metals incorporated in the pillared clay structure are crucial and make them suitable for a number of different applications, most of them belonging to the so-called "green chemistry" [118].This way, pillared clays have been tested as catalysts in different oxidation processes.Distribution of the pollutants studied in the literature corresponds mainly to phenol (22%), orange II and other dyes (23%), and only 5% to industrial effluents [118].Table 3 summarizes the research related to the use of pillared clays in wet hydrogen peroxide catalytic oxidation (WHPCO) of industry wastewater.The studies have basically focused on real olive oil milling wastewater (OMW).The reason for studying an olive oil mill wastewater effluent is because it constitutes a critical environmental problem in the Mediterranean area that accounts for 95% of the world olive oil production [128] and generates 30 million m 3 of OMW every year [129].
Most treatment studies of OMW are focused on aerobic biodegradation or anaerobic digestion of that material [130].Unfortunately, due to the effluents' high toxicity and biodegradability, no satisfactory results were obtained by these treatments.Indeed, in order to remove the organic compounds and reduce the methanogenic bacteria inhibition of the OMW, the WHPCO as pretreatment has been researched [83,131].
Tyrosol, p-coumaric, and p-hydroxybenzoic acids have also deserved the attention of some researchers because they are representative compounds of the polyphenolic fraction typically found in olive processing wastewater [69,70].The polyphenolic compounds conversion in OMW using Cu-PILC and Fe-PILC is about 45% and similar for the two catalysts.Therefore, the rate of polyphenolic conversion is around two to three times higher than the rate of TOC abatement, indicating a preferential reduction of these toxic compounds with respect to TOC abatement, in agreement with the goal of toxicity reduction, to improve biodegradability and to minimize as much as possible TOC reduction, because the OMW after WHPCO should go through a biological treatment [69].

Environmental Problems Associated with Coffee Wet Processing in Colombia
Coffee is one of the largest agricultural-based products sold in international markets and has become the second best-marketed product worldwide, surpassed only by oil [132].Colombian coffee has become a benchmark for the rest of the world due to the country unique weather and topography, wet processing, and programs to guarantee the coffee's origin.Colombia, the largest producer of washed Arabica coffee worldwide, counts an estimated average coffee production rate of 11 million bags per year (1 bag equals 60 kg of dried coffee beans), with coffee grown by a total of 553,000 families covering an area of 914,000 hectares [133].
The coffee fruit consists of a coloured exocarp (skin), a fleshy, yellowish-white mesocarp (pulp), mucilage layers (covering the two beans) and two coats (the first, a thin, fibrous parchment and the second, a fine membrane, silver skin) [134].Primary coffee processing refers to the processing of the coffee fruit to obtain coffee beans (also called green coffee beans at this stage).Coffee beans are extracted using one of three different methods: dry processing, wet processing, or semidry processing [135].Although all methods aim at removing the fruit flesh of the coffee cherry, each method removes the flesh in different ways [136].In the wet method process, which requires the use of specific equipment and substantial amounts of water, the pulp is eliminated using a pulper, followed by the mucilage's removal by natural fermentation.When fermentation is complete, the wet processed seeds are washed and dried, and the final product is "washed" or "parchment" coffee.Coffee processed using the wet method typically increases the market value of the beans because of its ability to add a soft acidity, good body, and better flavour [135,136].
The mucilage, a by-product of coffee wet processing, is primarily composed by pectin, sugars, water [136,137], and minor compounds such as tannins, caffeine, and phenols [138].It is estimated that for every 1 × 10 6 parchment bags, approximately 55.5 × 10 6 kg of fresh mucilage is generated, resulting in highly polluted nearby tributary water sources where the waste is discharged [139].For this reason, the National Coffee Research Center-Cenicafé has developed modular anaerobic treatment systems (SMTA, the Spanish acronym) using hydrolytic acidogenic and methanogenic reactors to treat coffee processing wastewater prior to its release, reducing the chemical oxygen demand (COD) by approximately 87%.The remaining 13% of the COD in the effluent is nondegrading and corresponds to recalcitrant compounds, including phenolic compounds [140].Treatment of coffee wastewater in a UASB (upflow anaerobic sludge blanket) reactor showed similar results: between 14% and 44% of phenolic compounds were removed during anaerobic digestion and, on average, 8% of the COD remained in the effluent and was not degraded [138].Because the SMTA effluent still poses toxic consequences for aquatic life, the effluent must go through posttreatment before being discharged into any water source.
To determine the biological impact generated by coffee wet processing wastewater, the National Coffee Research Center of Colombia-Cenicafé conducted bioassays using Chlorella vulgaris (algae), Daphnia pulex (microcrustacean), and Lebistes reticulatus (fish).It was found that the effluent from coffee wastewater can be toxic to the ecosystem at concentrations above 300 ppm of COD, inflicting a 50% mortality of fish, 43% of microcrustaceans, and 40% of algae, a negative impact on the aquatic ecosystem [141].
To complement the biological treatment, an integrated biosystem that uses macrophytes for posttreatment for coffee wet processing wastewater has been proposed, in order to generate the least negative impact on the coffee aquatic ecosystem [139].However, the biomass produced in these aquatic systems should be properly utilized in other processes such as the production of fertilizers, animal feed, and mushroom growing.
Considering that coffee wastewater is highly biodegradable, biological treatments have been widely used [142][143][144].In Colombia, a posttreatment for coffee wastewater is usually not performed because the elimination of 80% of the initial Table 3: Some examples of WPHCO of water pollutants (model molecules) or real effluents, using a Fenton reaction catalyzed by pillared clays.

Clay catalyst Organic pollutant Performances reached Reference
Al/Fe-PILC Treatment of municipal leachate from landfill by catalytic wet peroxide oxidation using an Al/Fe-pillared montmorillonite This advanced oxidation process has enhanced the biodegradability index of the leachate from 0.135 to 0.321 in 4 h No copper leaching during the oxidation reactions [73] COD in wastewater that is required by Colombian legislation can be achieved with the SMTA developed by Cenicafé [145].

WHPCO with AlFe-PILC as a Posttreatment System
for Coffee Wet Processing Wastewater.The effluent from the anaerobic biological treatment of coffee wet processing wastewater (CWPW) developed in the National Coffee Research Center-Cenicafé still contains a nonbiodegradable compound that must be treated before it is discharged into a water source.It was found that chlorogenic acid, caffeic acid and tannins are toxic compounds because they inhibit the process of methanogenesis and limit the biodegradability of water during anaerobic digestion [138].In the research group of "Solid State and Environmental Catalysis," the CWHPO using pillared clays with Al-Fe as catalysts was tested as a possible posttreatment system for coffee wet processing wastewater.
The physical chemical characterisation of the CWPW is detailed in Table 4.In general, with the use of the SMTA developed at Cenicafé, many of the contaminants present in wastewater as a result of the coffee wet processing were eliminated.However, the effluent of SMTA still retained an organic load that generated negative ecological impacts when deposited in the water bodies of the Colombian coffee zone, making posttreatment systems necessary.
A preliminary analysis of the phenolic compounds in CWPW was performed by HPLC-ESI-MS n (highperformance liquid chromatography/electrospray ionization multiple mass spectrometry) by using a Shimadzu Liquid Chromatograph-Ion Trap-Time of Flight Mass Spectrometer (LCMS-IT-TOF, Kyoto, Japan).To conduct the analysis, 100 mL of WPCW were filtered through Millipore filters (0.45 μm), and phenolic compounds were selectively obtained through successive extractions with 10 mL of ethyl acetate.Separation was performed in a Zorbax-SB C18 column (250 × 4.6 mm i.d., 5 μm, Phenomenex, USA).CWPW analysis (pos-biological treatment and precatalytic oxidation) using HPLC-ESI-MS n permitted a preliminary identification of compounds in the sample to compare with the values of m/z (molecular ion and fragments) reported in the literature [146,147].The results showed that the CWPW contains mainly chlorogenic and hydroxycinnamic acids: 3-O-p-coumaroylquinic acid (3-pCoQA), 4-O-p-coumaroylquinic acid (4-pCoQA), and 5-O-p-coumaroylquinic acid (5-pCoQA), and caffeic and ferulic acids.These compounds have also been found in green coffee beans [146].
Considering the results of the analysis of phenolic compounds in CWPW, the catalytic activity of the pillared bentonite with Al-Fe was evaluated in the oxidation reaction of three phenolic acids which were used as model molecules, using a similar procedure as that reported in previous studies for phenol [46,49,56,57].The phenolic acids used were chlorogenic (3-caffeoylquinic or 3-CQA), caffeic, and ferulic acids (Figure 1) at the following concentrations: 100 mg/L for 3-CQA and 40 mg/L for both caffeic and ferulic acids.
Traditionally, the tests with phenol as a model system are performed with 40 mg/L but, for 3-CQA, a higher concentration was used, given that 3-CQA is one of the most abundant compounds in the CWPW [138].Monitoring of the phenolic acid conversion over time was conducted by high performance liquid chromatography (HPLC) using a Hitachi D-7000 (LaChrom) instrument equipped with a LiChrospher 60 Select B column, absorbance detector UV-vis L-7400, and, as a mobile phase, using a mixture of H 2 O, H 3 PO 4 , and CH 3 OH.The retention times for chlorogenic, caffeic, and ferulic acids were 9.3, 9.9 and 11.0 min, respectively.Selectivity of the catalysts toward CO 2 and H 2 O was evaluated using the analysis of total organic carbon (TOC) by an AnaTOC equipment.
Wet hydrogen peroxide catalytic oxidation tests of Al-Fe-PILC with chlorogenic (3-CQA), caffeic, and ferulic acids showed a beneficial effect when incorporating Fe in the bentonite, both with respect to the phenolic acids conversion (Figure 2) and total organic carbon, TOC (Figure 3).The conversion of phenolic compounds using the catalyst Al-Fe-PILC was higher for the chlorogenic acid (91%), followed by caffeic acid (87%) and ferulic acid (78%) after 4 h.Although a complete oxidation of these acids was not achieved, conversions higher than 78% are considered to be very good considering the complex structure of these compounds and the fact that the reaction was performed at 25 • C. Selectivity of the Al-Fe-PILC towards CO 2 and H 2 O was higher for the chlorogenic acid (49%), followed by the caffeic acid (37%) and ferulic acid (34%).These results are associated with differences in chemical structure.It was also found that the reactivity of phenolic compounds can be drastically affected by the electronic nature of substituents and by their positions in the aromatic ring [148,149].The high conversion of chlorogenic acid can be explained by the presence of two electron-donating groups (as caffeic acid) and by three additional hydroxyl groups in the ester moiety.An example of the relationship between the chemical structure and its reactivity with hydroxyl radical would be the wet hydrogen peroxide photo-oxidation of p-coumaric, vanillic, ferulic, and caffeic acids when catalysed by (Al-Fe)-PILC, in which the degradation of phenolic compounds was 28%, 50%, 58%, and 86%, respectively [148].Because the hydroxyl radicals generated by the catalytic decomposition of H 2 O 2 are both reactive and nonselective, the attack position in the aromatic ring can be located anywhere on the ring [150].Therefore, the catalytic degradability of some phenolic compounds has been correlated by the Hammett constant (σ), explaining the effect that different substituents have on the electronic character of a given aromatic system (electronwithdrawing or electron-donating groups) [148,150].The reaction scheme for evaluating the catalytic activity of AlFe-PILC in the oxidation of the CWPW was described in Figure 4.For testing, the semibatch reactor thermostated at 25 • C was loaded with 100 mL of coffee wastewater, 0.5 g of the catalyst, 0.1 M hydrogen peroxide solution (10 mL, 2 mL/h), bubbling air at a constant flow (2 L/h), and brought to a pH of 3.7 with 0.1 M NaOH or HCl.The total phenol conversion in CWPW was performed using the Folin-Ciocalteu colorimetric method [151,152].Selectivity of the catalysts toward CO 2 and H 2 O or grade of mineralization was evaluated using the analysis of total organic carbon (TOC) with an AnaTOC equipment.The AlFe-PILC achieved a high conversion rate of total phenolic compounds (56%) and mineralization towards CO 2 and H 2 O (40%) after 5 h reaction time (Figure 5).The starting bentonite (Bent) used as a reaction target achieved a conversion rate of phenolic compounds of 8% and selectivity to CO 2 and H 2 O of 4%, values attributed to the activity of iron in the starting clay (8.2% Fe 2 O 3 ).The chemical oxygen demand (COD) of coffee processing wastewater after wet hydrogen peroxide catalytic oxidation was reduced in 60%.In terms of stability of the active phase, an average concentration of leached iron was <0.22 ppm.
The combination of the two treatment methods, biological (developed by Cenicafé) and catalytic oxidation with AlFe-PILC, achieved a 96.7% reduction of COD in CWPW.These results are higher than those obtained when International Journal of Photoenergy coffee processing wastewater was treated by coagulationflocculation in combination with advanced oxidation processes (UV/H 2 O 2 /O 3 ), where a COD reduction of 87% was achieved [153].Therefore, the WHPCO using AlFe-PILC catalysts is a viable alternative for the posttreatment of coffee processing wastewater.

Conclusions
The pillared clays have received considerable attention in the last decade as catalysts for wet hydrogen peroxide oxidation of phenolic compounds because of their high activity (conversion of phenolic compound and degree of mineralization) and environmental compatibility (low cost, easy recovery of the catalyst, are reusable catalysts, oxidation that can be carried out at room temperature and atmospheric pressure, and that they show high stability of the active phase in the reaction medium).With the developments in the synthesis of pillared clays, it has been possible to reduce the water volume and synthesis times, and technical requirements for these materials can be useful at industrial level.
Given the excellent properties of pillared clays in the WHPCO for phenol and other model molecules, as well as real wastewater treatment, this advanced oxidation process can be integrated with biological process, as a pre-or posttreatment, depending on the physicochemical characteristics of the wastewater.
The high conversion of phenolic compounds, the selectivity to CO 2 and H 2 O, and stability of the active phase in the pillared bentonite with Al-Fe in the WHPCO of coffee wastewater show the potentiality of this catalyst for posttreatment for this type of effluent.The combination of the two treatment methods, biological and catalytic oxidation with AlFe-PILC, achieved a 96.7% reduction of chemical oxygen demand in coffee wet processing wastewater.

Table 1 :
Bentonite-based catalysts for oxidation of phenol using Fenton-like AOPs.

Table 2 :
Some examples of pillared clays-based catalysts for wet hydrogen peroxide oxidation of phenol.

Table 4 :
Physicochemical parameters of coffee wet processing wastewater before and after biological treatment in SMTA.