Photocatalytic Bactericidal Efficiency of Ag Doped TiO 2 / Fe 3 O 4 on Fish Pathogens under Visible Light

1 International Postgraduate Program in Environmental and Hazardous Waste Management, Chulalongkorn University, Bangkok 10330, Thailand 2National Center of Excellence for Environmental and Hazardous Waste Management, Chulalongkorn University, Bangkok 10330, Thailand 3Department of Chemical Engineering, Faculty of Engineering, Thammasat University, Bangkok 12120, Thailand 4Center of General Education, MingDao University, Changhua 52345, Taiwan 5Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan


Introduction
Bacterial pathogens are a main cause of fish mortalities in cultured fish and occasionally in wild fish.As facultative pathogen exists for both fish and human [1,2], human infections caused by pathogens transmitted from fish or aquatic environment are quite general.Such infection varies by seasons, dietary habits, and the immune system status of the exposed individual.Traditional methods such as chlorination are chemical intensive and have many disadvantages.For example, chlorine used in water treatment for disinfection can react with organic material to generate carcinogenic chloroorganic compounds.Moreover, certain pathogens, bacteria, and protozoans have been known to be resistant to chlorine disinfection [3].
Applications of photocatalytic processes are viable solutions to environmental problems.Heterogeneous photocatalytic oxidation (PCO) has been proposed as one of the advanced oxidation techniques for mineralization of hydrocarbon pollutants.PCO helps to create strong oxidation agents that breakdown organics to CO 2 in the presence of the photocatalyst, H 2 O, and light.Heterogeneous systems also have the advantages of minimal waste generation and reusability of catalysts.
TiO 2 , one of the most promising semiconductor photocatalysts for removing pollutant and cleaning water, is of low cost, nontoxic, and physically and chemically stable.TiO 2 particles are both photocatalytic and antimicrobial [4].Anatase TiO 2 is superior to rutile and brookite for organic compound removal.However, its wide band gap (3.2 eV) 2 International Journal of Photoenergy allows it to be activated only by UV.Also, the high rate of electron-hole (e − -h + ) recombination in TiO 2 particles results in low photocatalytic efficiency.Doping TiO 2 with various transition metal ions narrows the band gap [5] and expands the photoresponse of TiO 2 into visible spectrum [6].Also, electron transfer between those metal ions and TiO 2 can alter e − -h + recombination as the metal ions are incorporated into the TiO 2 lattice.Feng et al. [7] have studied the antibacterial mechanism of Ag + on bacteria and found that Ag + deprives DNA molecules' replication abilities.In addition, Ag + increases visible light absorption and holds up the e − -h + recombination of TiO 2 .
Many of the particles used in the separation technology are superparamagnetic, which can be magnetized with an external magnetic field and redispersed upon the removal of magnet [8,9].For example, the magnetic property Fe 3 O 4 helps to enhance the recovery of the catalyst via external magnetic field.Magnetic Fe 3 O 4 particles suspended in carrier fluids are referred to as magnetic fluids.Additionally, Fe 3 O 4 also enhances the visible light absorption which resulted from the band gap reduction [10].

Synthesis of Ag-TiO
The Ag-TiO 2 /Fe 3 O 4 magnetic photocatalyst was synthesized in two steps.First, magnetite (Fe 3 O 4 ) nanoparticles were synthesized via coprecipitation method, in which FeCl 3 anhydrous and FeCl 2 ⋅4H 2 O salts in the molar ratio of 2 : 1 were dissolved and vigorously stirred in double-distilled water.NaOH was then dropped slowly into the solution until a large amount of black precipitates formed.The resulting precipitates were collected using a magnet and washed several times with double-distilled water and ethanol.The reaction in this process is as follows: In the second step, Ag-TiO 2 /Fe 3 O 4 composite particles were prepared using modified sol-gel method.Acetylacetone (Acac), as a chelating agent, was added into isopropyl alcohol and stirred magnetically.Titanium isopropoxide (TTIP) was then added gradually to the mixture with Acac : IPA : TTIP in molar ratio of 2.84 : 23.64 : 1.An appropriate volume of aqueous silver nitrate was added to the titanium solution to attain 1 wt% of Ag on TiO 2 and stirred vigorously for 30 minutes.Meanwhile, Fe 3 O 4 particles were dispersed in isopropyl alcohol and sonicated in an ultrasonic apparatus for 10 minutes.Thereafter, slowly add the mixture of Ag-TiO 2 into Fe 3 O 4 suspension with TTIP : Fe 3 O 4 ratio of 10 (weight basis) and stir the mixture at room temperature for 3 hours to ensure uniform composition.The obtained suspension was placed in a hot air oven at 90 ∘ C for the particles to dry.Finally, the composite particles were calcinated in oxygen at 450 ∘ C for 3 hours to form Ag-TiO 2 /Fe 3 O 4 .

Characterization of Ag-TiO
The properties of Ag-TiO 2 /Fe 3 O 4 magnetic photocatalyst were characterized with various instruments.The crystal structure of the particles was characterized with X-ray diffraction (XRD) on a Bruker AXS diffractometer with CuK radiation.The X-ray was generated with a current of 40 mA and a potential of 40 kV in angular range (2) from 10 ∘ to 80 ∘ .The UV-Visible diffuse reflectance spectra in the range of 320-800 nm were acquired from a Hitachi U3501UV-visible diffuse reflectance spectrophotometer (UV-Vis DRS) equipped with integrating sphere.Pure BaSO 4 powder was used as a reflectance standard.Transmission electron microscopy (TEM) observation of the samples was performed on a HITACHI 7500 transmission electron microscope operated at 80 kV.The magnetization of photocatalyst was measured using superconducting quantum interference device (SQUID).

Evaluation of Photocatalytic Bactericidal Activities.
The research team has evaluated photocatalytic bactericidal activities using modified antibacterial drop test.In order to differentiate the effect of TiO 2 and silver, the experiment was conducted both under visible light and in dark to block photocatalytic process to assure that the bactericidal activity is exclusively from silver.Fish pathogens used in the experiment included Aeromonas hydrophila (BCRC13018), Edwardsiella tarda (BCRC10670), and Photobacterium damselae subsp.piscicida (BCRC17065).Bacterial cells were collected via centrifugation at 9500 rpm for 10 minutes to remove supernatants.The pellets were then rinsed twice with 15 mL phosphate buffer saline (PBS, 137 mM sodium chloride; 10 mM phosphate; 2.7 mM potassium chloride; pH 7.4).Ten mL of pathogens in PBS was added to each 6 cm diameter sterilized glass Petri dish containing various amounts of Ag-TiO 2 /Fe 3 O 4 and irradiated with visible fluorescence light (FL40S⋅N-EDL⋅NU, Mitsubishi/Osram, Japan,  > 420 nm, 1040 lux) for various time intervals.The control groups were without photocatalyst.All control and experimental groups had 3 replicates.At each time interval, 20 L of such solution was transferred from each Petri dish to 96-well plate containing 180 L of 0.05% 2,3,5-triphenyl tetrazolium chloride (TTC) and incubated at 28 ∘ C for 10 hours.The absorbance of each tube at 540 nm was measured with ELISA reader after adding 50 L of isopropanol to the tubes to terminate reaction [11].
The inhibition efficiencies to fish pathogen were calculated as where [Abs]  is the absorbance of control group at certain time interval and [Abs]  is the absorbance of experimental group at the same time interval.
International Journal of Photoenergy

Results and Discussion
Figure 1 displays the XRD patterns of P25 TiO ).With no evidence of its correspondence to the rutile phase, the patterns correspond to the anatase phase exclusively at the calcination temperature of 450 ∘ C.These results help to conclude that the presence of the iron oxide has no accelerating effect on the anatase-rutile phase transformation of the TiO 2 .In addition, there is no characteristic peak of Ag presented in the pattern, which implies that the amount of Ag particles is not adequate to present their characteristic patterns [12,13].The pattern of Fe 3 O 4 does not appear in the XRD, which may indicate that Fe 3 O 4 is encapsulated by Ag-TiO 2 .However, the broadened patterns suggest that Ag + doping suppresses the growth of TiO 2 crystals.The average crystallite size () of catalyst is estimated using Scherrer's equation: where  is crystallite size (nm),  is crystallite shape factor (0.90),  is X-ray wavelength, for CuK (0.15418 nm),  is the full-width-half-maximum (FWHM) of the peak, and  is Bragg angle.
The crystallite size can be measured via the diffraction data in Figure 1 according to the Scherrer equation for the peak at 25.3 ∘ .The estimated size is about 12.53 nm.
Figure 2 shows the influence of Ag and Fe 3 O 4 on the UV-Visible light absorption.Fe 3 O 4 slightly enhances the visible light absorption of TiO 2 .With the modification of Ag, the shifting of Ag-TiO 2 absorption spectrum to longer wavelengths is noticeable, which is due to the interaction between Ag and TiO 2 matrix.In addition, Ag-TiO 2 /Fe 3 O 4 demonstrates significantly higher absorption in the 400-800 nm range.As shown in Figure 3, UV-Vis absorption spectra are converted to the Tauc plot of (h]) 1/2 and photon energy, and the linear extrapolations are made by drawing a tangent line through the maximum slope and taking its intersection with X-axis at (h]) 1/2 = 0 [14].
The calculated energy band gaps of TiO 2 /Fe 3 O 4 , Ag-TiO 2 , and Ag-TiO 2 /Fe 3 O 4 are 2.9, 2.7, and 2.35 eV, respectively (Figure 3).Compared to the original anatase TiO 2 band gap of 3.2 eV, Fe 3 O 4 and Ag dopants have improved the photocatalytic activity under visible light via narrowing the band gap and enhancing the visible light absorption of TiO 2 (Table 1).
Figure 4 shows the XPS spectra for Ag3d region of Ag-TiO 2 /Fe 3 O 4 .The spectra consist of two peaks at around 367 and 373 eV, which correspond to Ag3d 5/2 and Ag3d 3/2 , respectively.The peaks are slightly broadened and can be considered as the sum of multiple overlapping peaks.As the XPS infers, the silver species on the Ag-TiO 2 /Fe 3 O 4 photocatalyst are metallic silver and silver ions coexisting in terms of the bonding energy corresponding to Ag3d 5/2 of metallic Ag (Ag 0 , 368 eV), Ag 2 O (Ag + , 367.5 eV), and AgO (Ag 2+ , 367 eV), respectively [15,16].
Figure 5 shows the morphology of the sample under TEM.The Ag-TiO 2 /Fe 3 O 4 particle connects tightly to one another.The diameter of the particles is in the range of 14-40 nm.
Figure 6 displays the energy dispersive X-ray (EDX) spectra analysis of Ag-TiO 2 /Fe 3 O 4 and Table 2 lists the  composition of the prepared photocatalyst.Among the four elements (Ti, Fe, O, and Ag) presented, higher Ti content compared to magnetite could be resulting from the formation of TiO 2 layer coated on Fe 3 O 4 particles.The Ag signals are around 2.8 keV, which may indicate the existence of Ag particles in catalyst.
Figure 7 displays the magnetic property of Ag-TiO 2 /Fe 3 O 4 measured at 25 ∘ C. The absence of hysteresis loop of the sample indicates the superparamagnetic character of the material [17].Figure 8 shows the synthesized Ag-TiO 2 /Fe 3 O 4 (with a saturation magnetization of 2.7 emu/g) being recollected from the solution with a magnet.
Figure 9 shows the indigo carmine decolorization efficiency of TiO    The results suggest that Ag deposition has enhanced the visible light photocatalytic activity of TiO 2 .Such enhancement may be attributed to the electron interaction at the contact between the metal deposits and the semiconductor surface.The Ag deposits act as e − traps that immobilize the photogenerated electrons.The trapped electrons are then transferred to oxygen to form highly oxidative species such as O 2 − .The Fe 3 O 4 in the photocatalyst helps to enhance the visible light activity of TiO 2 .As TiO 2 is the active site of the catalyst, substituting Ag-TiO 2 with Fe 3 O 4 may decrease the photocatalytic activity.Although Ag-TiO 2 demonstrates higher decolorization efficiency than Ag-TiO 2 /Fe 3 O 4 , yet Ag-TiO 2 is not recollectable with magnet after dispersing in  water.As a result, only Ag-TiO 2 /Fe 3 O 4 is used for bactericidal efficiency evaluation.The catalyst reusability is an important parameter for practical applications.The repetitive use of as-synthesized Ag-TiO 2 /Fe 3 O 4 was studied for different cycles of indigo carmine decolorization under visible light irradiation.The catalyst was recovered using a permanent magnet bar and used for three cycles with all other parameters kept constant.The results demonstrate that Ag-TiO 2 /Fe 3 O 4 maintains good activity in three runs, with only a small loss.The drop in decolorization might be due to the loss or aggregation of the particles during the recycling process.The decolorization after 6 h irradiation at the third run (Figure 10) was around 85%, which indicates that Ag-TiO 2 /Fe 3 O 4 sustains well from recycling and has good potential for practical applications.
The research team tested three Ag-TiO 2 /Fe 3 O 4 loadings (i.e., 3.5 mg, 2.7 mg, and 1.8 mg) to select its suitable one in 10 mL of phosphate buffer saline (PBS) containing fish pathogens.As displayed in Figure 11, the antibacterial efficiencies are less than 10% in all loadings for all catalysts within the first 30 minutes.After that, the specimen of 3.5 mg Ag-TiO 2 /Fe 3 O 4 load demonstrates sharp efficiency increase to reach ∼100% after 90 minutes.The specimen of 2.7 mg load demonstrates increased antibacterial efficiency after 60 minutes to reach 95% after 120 minutes.The specimen of 1.8 mg load demonstrates very low antibacterial efficiency, only 18% fish pathogens degradation.Therefore, 2.7 mg load was chosen for further study.
The experiment uses Aeromonas hydrophila (BCRC-13018), Edwardsiella tarda (BCRC10670), and Photobacterium damselae subsp.piscicida (BCRC17065) as target bacteria.These bacteria, which cause losses in wild and farmed fish stocks, are gram negative fish pathogens that inhabit in freshwater as well as seawater.As shown in Figure 12, the effects have been slow in the first 30 minutes and then start to increase more significantly.Almost all bacteria were destroyed by Ag-TiO 2 /Fe 3 O 4 after 120 minutes irradiation.The bactericidal efficiency is about 20% for both BCRC13018 and BCRC17065 after 60 minutes of visible light irradiation.
No significant difference has been found between these two pathogens.After 120 minutes, the bactericidal efficiencies increase to 93% for BCRC13018 and 81% for BCRC17065.The results suggest that Photobacterium damselae subsp.piscicida is more resistant to Ag-TiO 2 /Fe 3 O 4 among these three pathogens.
Figure 13 shows that in the dark, where no photocatalysis process occurs, Aeromonas hydrophila and Photobacterium damselae subsp.piscicida degrade ∼15% after 120 minutes, while Edwardsiella tarda degrades more quickly and reach 100% within 120 minutes.The bactericide effect seems to be exclusive due to the presence of Ag + .Such findings indicate that Edwardsiella tarda is a very sensitive microorganism and more susceptible to silver particle.These results suggest that Ag-TiO 2 /Fe 3 O 4 's photocatalytic bactericidal effect is species dependent.

Conclusions
Ag-TiO 2 /Fe 3 O 4 magnetic photocatalyst has demonstrated strong antimicrobial properties through a mechanism including photocatalytic production of reactive oxygen species   that damage cell components and viruses [18].The holes on the valence band of TiO 2 can react with either H 2 O or OH − absorbed on the surface to produce hydroxyl radicals and with the electrons on the conduction band to reduce O 2 to produce superoxide anion.The detection of other reactive oxygen species such as H 2 O 2 and singlet oxygen has also been reported.Hydroxyl radical and superoxide anions, both known to be highly reactive with biological samples, are considered the main species generated in the anodic and cathodic pathways, respectively, of photocatalytic processes in the presence of oxygen.The XPS data indicated different silver species coexisting in the Ag3d 5/2 region of  photocatalyst with binding energies at around 367 eV and 368 eV, assigned to silver ion (Ag + and/or Ag 2+ ) and metallic silver (Ag 0 ), respectively.Ag + can cause severe alterations to bacteria via binding to bacterial denatured DNA and RNA so as to inhibit the replication.The modifications of membrane structure that include changes in membranebound enzyme activities, metabolic pathways, transport systems, and permeability alterations lead to cell death.Ag + from Ag-TiO 2 inactivates membrane proteins and respiratory enzymes.Reactive oxygen species damage cell membrane when cell comes into contact with catalyst surface.Ag also acts as the trap of photogenerated electrons to prevent the e −h + pairs from recombining rapidly after photoexcitation.
As a final remark, the research team intend to use light emitting diodes (LEDs) of different colors as the light source to identify the most responsive spectrum of Ag-TiO 2 /Fe 3 O 4 for the future study since high intensity LEDs have become prominent light sources for scientific research [19][20][21].

Figure 2 :
Figure 2: UV-Vis diffuse reflectance spectra for the as-synthesized samples.

Figure 10 :
Figure 10: The reusability of Ag-TiO 2 /Fe 3 O 4 as demonstrated in decolorization of indigo carmine under visible light irradiation.

Table 1 :
Estimated band gap energy of as-synthesized samples.