Synthesis and Controlled Release Behavior of Biodegradable Polymers with Pendant Ibuprofen Group

The continuous use of nonsteroidal anti-inflammatory drugs such as ibuprofen frequently leads to some serious side-effects including stomach ulcers and bleeding. In this paper, two kinds of new biocompatible polyesters (PIGB, PIGH) and polyesteramide (PIGA) comprising biodegradable components (L-glutamic acid, 1,4-butanediol, and 1,6-hexanediol and 6-amino hexanol) and ibuprofen as pendant group have been prepared by themelting polycondensation.The chemical structures of themonomer and polymers are characterized by FTIR, H NMR spectrum, GPC, and contact angle measurements. The drug loading of ibuprofen reaches very high level (35–37%) for PIGB, PIGH, and PIGA carriers. The free ibuprofen molecules are released in vitro from polymer carriers in a controlled manner without a burst release, different from the release pattern observed in the other drugencapsulated systems. It is also found that the different hydrophilicity among PIGB, PIGH, and PIGA plays a key role in the timecontrolled release of ibuprofen. In addition, the viability of HeLa cells after 48 h of incubation reaches more than 100%, indicating no cytotoxicity for PIGB, PIGH, and PIGA carriers.


Introduction
As a common nonsteroidal anti-inflammatory drug, ibuprofen is usually used to treat rheumatoid arthritis, osteoarthritis, psoriatic arthritis, and some pain/swellings in ankylosing spinal cord inflammation [1,2].It has been reported that the half-life of ibuprofen in the blood is only 2.1 hours, so frequent use of this drug is needed by the patients [3].However, some serious side-effects of ibuprofen could be initiated in various parts of the body (including disease part and nondisease part) as the drug use is used frequently [4,5].For example, the patients would suffer from stomach ulcers, bleeding, and other pains with persistent usage of this drug.
In recent years, many developments in the drug delivery system have been focusing on the target release [6], controlled release [7], extended time of drug action [8], and so on.For ibuprofen, several methods for the preparation of drug carriers have been reported to solve the issues appearing in the direct use of drug.For example, polymer carrier of ibuprofen was prepared by the electrospinning of poly(trimethylene carbonate-co--caprolactone) [9].The mono/multifilament sutures, made from ibuprofen-loaded poly(p-dioxanone), were fabricated through a molecular diffusion process using a swelling agent such as dichloromethane [10].Molten ibuprofen was successfully loaded into the large spherical particles of amorphous microporous silica in the adsorption process [11].Moustafa and coworkers also described a differential microemulsion polymerization technique to produce ibuprofen-entrapped nanoparticles, comprising copolymer from methyl methacrylate/hydroxyethyl methacrylate [12].Compared with the traditional system, the design and preparation of these drug deliveries can reduce the sideeffects and effectively increase the duration time of drug.However, the main problems of the drug release systems still exist, that is, lower drug loading (less than 30%), explosive release, and rapid drug release.A variety of drug carriers with low ibuprofen loading ratio were reported, using electrospun fibers [9], Pluronic mixture gels [13], copolymer membrane of ethylene vinyl acetate [14], and polymer-drug complexes [15].The loading ratio of the drug 2 International Journal of Polymer Science is around 1-23%.In addition, the widely used materials in the drug systems are usually acrylic/vinyl polymers with C-C bond in their main-chains such as poly(methyl methacrylate/hydroxyethyl methacrylate) [12], polyethylene [16], poly(2-hydroxypropyl acrylate) [17], poly(hydroxyethyl methacrylate) [18], poly(methacrylic acid) [19], poly(Nisopropylacrylamide) [20,21], and poly(vinylpyrrolidone) [22].These polymers are not biodegradable or biocompatible in nature.Therefore, the used polymers would be retained in the human body after the drug is released, resulting in the potential harm and adverse effects [4].
Developing the biodegradable polymer as drug carriers can reduce organ toxicity, side-effects of drugs, and possible harms of residual polymers in body.Over the years, some researchers such as Jia and Kerr [23], Lin et al. [24], del Valle et al. [25], Rosario-Meléndez et al. [26], and Cantón et al. [27] have paid close attention to the investigation of drug carriers comprising biodegradable polymers.Kerr and coworkers described an ibuprofen carrier using a composite of biodegradable poly(lactic-co-glycolic acid) and TiO 2 nanotubes with much improved mechanical strength and flexibility [23].Lin et al. have prepared blend matrices for controlled drug release system using phosphorylcholinefunctionalized poly(caprolactone) with good biodegradability and biocompatibility [24].del Valle et al. also fabricated ibuprofen-loaded porous matrices using biodegradable poly(ester amide) derived from sebacic acid, alanine, and 1,12dodecanediol [25].In addition, the biodegradable polyglycolide is another widely used material for uniformly drug-loaded electrospun fibers [27].

Materials and Methods
Contact angle measurements of polymers were performed at room temperature in air atmosphere by the sessile drop method using a goniometer DataPhysics OCA 15EC.The samples were prepared by melting method: first heating the sample on a glass plate at about 130 ∘ C and then cooling to room temperature for 1 h to cast sample films..The films were kept at the desired temperature at least 30 min before the measurement.In the contact angle measurements, the angles were recorded 2 seconds after 2 L of water was dropped on the film.The final contact angle for each sample was calculated from the average value of five experimental results.
Cytotoxicity was evaluated using the CCK-8 Cell Counting Kit assessment in this investigation.The HeLa cells grew in a CO 2 incubator with a humidified 5% CO 2 atmosphere at 37 ∘ C overnight.HeLa cells were maintained in RPMI-1640 (GIBCO) cell supernatant containing 10% inactivated fetal bovine serum (FBS), 100 g/mL penicillin, and 100 g/mL streptomycin.For the assay, cells were plated at 5 × 10 5 cells/100 L/well in a 96-well plate.The cells were preincubated with serum-containing regular medium, which contains sample (0.01 g/mL, 0.1 g/mL, 1.0 g/mL, 10 g/mL, and 100 g/mL) at 37 ∘ C for 48 h.CCK-8 solutions (10 L) were added to each well, followed by incubation at 37 ∘ C for 1 h.The medium was removed and 100 L of acidulated isopropyl alcohol was added to each well to dissolve the purple crystals.The plate was read for absorbance at 450 nm.
In this study, we prepared a series of phosphate buffer solution of ibuprofen (pH = 7.6) with the concentration including 10 g/mL, 5 g/mL, 2 g/mL, 1 g/mL, and 0.5 g/mL using a stepwise dilution method.A working curve was plotted using the maximum absorption (at 220 nm) as  value and the concentrations of ibuprofen as  value.The relationship between the absorption and the concentration of ibuprofen was obtained by the linear fitting.The equation is as follows:  = 0.0557 + 0.0399 (: absorbance, : concentration, and the linearly dependent coefficient  = 0.9986).The release of ibuprofen from PIGA, PIGB, and PIGH carrier was studied at 37 ∘ C and 25 ∘ C under the simulated physiological conditions (PBS, pH = 7.4).Triplicate samples of each polymer (about 50.0 mg powder) were placed in 20 mL vials together with 10 mL PBS under agitation (60 rpm).After predetermined release time, the samples were centrifuged at 2000 rpm for 6 min.The clear solution was collected and replaced with the fresh PBS (10 mL) for the subsequent release study.The amount of released ibuprofen is calculated by the absorbance at 220 nm according to the working curve.

Preparation of 2-Methyl-4-(2-methyl propyl)
Phenylacetyl Chloride.3.0 g 2-methyl-4-(2-methyl propyl) phenylacetic acid (ibuprofen) was added to a 100 mL round bottom flask with stirring.In the ice bath, the reaction system was cooled for 10 min and 10 mL thionyl chloride was then introduced into the reaction system.A large number of bubbles were generated and overflew from the flask.After 20 min reaction, the reaction system was changed into heating step and refluxed at about 65-75 ∘ C until no more bubbles were generated again in the flask.Lastly, the excess thionyl chloride was completely removed by rotating evaporation to get targeted compound, 2-methyl-4-(2-methyl propyl) phenylacetyl chloride.

Synthesis of N-2-Methyl-4-(2-methyl propyl) Phenylacetyl-Glutamic Acid (MMPPG).
In a 100 mL flask, 3.26 g 2methyl-4-(2-methyl propyl) phenylacetyl chloride was dissolved in 10 mL anhydrous tetrahydrofuran.In a 20 mL vial, 1.82 g L-glutamic acid was slowly dissolved in 3 mL NaOH aqueous solution (2 M).The ratio of L-glutamic acid to 2methyl-4-(2-methyl propyl) phenylacetyl chloride was set at 0.85 : 1.0 to make sure there is no L-glutamic acid left.After mixing the L-glutamic acid aqueous solution with 2-methyl-4-(2-methyl propyl) phenylacetyl chloride, the mixture was stirred for 8 h.After completing the reaction, the concentrated HCl was slowly dropped into the reaction system until the solution was acidified to pH 1∼3.A lot of white solids then appeared during the acidification.After filtration, the filtrate separated into two immiscible layers.Tetrahydrofuran in the upper layer was removed by rotating evaporation to get the crude product.The purified N-2-methyl-4-(2-methyl propyl) phenylacetyl-glutamic acid (MMPPG) was obtained via first washing with petroleum ether and subsequent washing with ether.The reaction about synthesis of MMPPG was shown in Figure 1.

Structural Characterization of Monomers and Polymers.
In this investigation, the chemical structures of PIGB, PIGH, and PIGA were confirmed by 1 H NMR, FTIR spectrum, and GPC.As indicated in Figure 2, PIGB, PIGH, and PIGA can show some characteristic peaks in FTIR spectra confirming presence of ester, amide groups, and benzene rings.For the representative FTIR spectrum of PIGH, the stretching vibration and deforming vibration of N-H linkage in the amide group are recorded at 3306 and 1535 cm −1 .The characteristic peaks at 1652 and 1737 cm −1 are attributed to the stretching vibration of carbonyl (C=O) in the amide and ester groups in PIGH, respectively.Furthermore, the ether linkage (C-O-C) from the ester groups in the PIGH main-chain can be identified at 1176 cm −1 , and the stretching vibrations for CH, CH 2 , and CH 3 in PIGH appear at 2954 cm −1 and 2860 cm −1 .Similarly, the characteristic peaks of benzene ring can be found at the vicinity of 3065 cm −1 .
Compared with polyesters (PIGB and PIGH), the most obvious structural change of polyester-amide (PIGA) is the two amide groups in the main-chain and pendant group.The peak at 1735 cm −1 belongs to the characteristic absorption of carbonyl (C=O) in ester group, and the peak at 1650 cm −1 is  ascribed to carbonyl (C=O) in the amide groups of PIGA.Furthermore, the intensity of the peak at 1735 cm −1 is much lower than that at 1650 cm −1 , which is different from FTIR of PIGB and PIGH.
The 1 H NMR spectra of PIGB, PIGH, and PIGA in deuterated chloroform (CDCl 3 ) were shown in Figure 3.For PIGB, the peaks at 7.11-7.20 ppm (d, c) are ascribed to four hydrogens from benzene ring of ibuprofen.The characteristic peak at 6.20 ppm (g) can be attributed to N-H from amide groups.The peaks of the tertiary carbon (C-H) appear at 4.55 ppm (h) and 3.58 ppm (e), respectively.Additionally, the absorption peaks of CH 2 next to O atom from 1,4-butanediol are recorded at 4.06 ppm (k).The other two CH 2 groups in 1,4-butanediol moiety appear at 1.73 ppm (l).And also, the peaks at 2.22 ppm (j) belong to the characteristic absorption of CH 2 groups in L-glutamic acid structure.The other peaks in the range of 2.5-0.8ppm (a, b, f) are corresponding to the CH, CH 2 , and CH 3 groups in ibuprofen moiety.It is necessary to state that 1 H NMR spectrum of PIGH is similar to PIGB, and the broad peaks of N-H in PIGA appear at 7.5∼8.5 ppm.Based on the abovementioned FTIR and 1 H NMR spectra, PIGB, PIGH, and PIGA have been successfully synthesized in our experiment.
According to the structure shown in Figure 1, the content of ibuprofen moiety in synthesized polymer carriers was calculated as 37.7% for PIGB, 35.2% for PIGH, and 35.3% for PIGA, respectively.By the polymeric drug or pendant drug methods, the drug-loading capacity was also greatly increased in some other reported systems such as malic acidbased polyester [28], poly(-caprolactone-co-lactide) [29], polyacrylic ibuprofen prodrug [17], ibuprofen carrier from polyethylene glycol [30], and polyethylene-based polymeric prodrugs [16].In this investigation, the molecular weights of three polymer carriers were measured by GPC measurement with N,N-dimethylformamide (0.1% LiBr) as the eluent.As shown in Figure 4(a), the molecular weights of PIGA, PIGB, and PIGH were 0.82 × 10 4 g/mol, 0.87 × 10 4 g/mol, and 0.75 × 10 4 g/mol, and their polydispersity index (PDI) was 1.87, 2.22, and 1.96, respectively.In addition, the contact angles measurement was used to confirm the relative hydrophilicity of polymers.Seen from the molecular structure, PIGA is the most hydrophilic compound because of one ester and two amides in the repeating unit, and its contact angle with water should be the smallest one.PIGH is most hydrophobic due to the two esters, one amide and the longer aliphatic chain, and its contact angle with water should be the largest one.As shown in Figure 4(b), the contact angles of PIGA, PIGB, and PIGH were 44 ∘ , 70 ∘ , and 82 ∘ at room temperature, respectively.The experimental results about contact angle measurements were in accordance with the abovementioned theoretical analysis.

Release Profile of Ibuprofen from PIGB, PIGH, and PIGA.
In this investigation, the different release behavior of ibuprofen was observed for polyester-amide (PIGA) and polyesters (PIGB, PIGH).As presented in Figure 5, the amount of ibuprofen released from PIGA chains is much higher than the other two polymers, which is 65% over 15 h at the simulated physiological conditions (37 ∘ C).At room temperature (25 ∘ C), the accumulative release of ibuprofen is about 50% in the same release time.This release difference can be attributed to the fact that the release rate of ibuprofen by the cracking of amide (hydrolyzing) at 37 ∘ C is faster than that at 25 ∘ C.
Figure 6 indicated the release behaviors of ibuprofen from polyesters PIGB and PIGH in the simulated physiological conditions.For polyesters PIGB and PIGH, the amount of released ibuprofen is 32% and 27% at 37 ∘ C over 10 days, respectively.At 25 ∘ C, the accumulative release of ibuprofen is measured as only 25% and 18% for PIGB and PIGH over the same time.For PIGA, PIGB, and PIGH, the ibuprofen is released from these polymer carriers in a controlled manner.In other words, the obvious burst is not observed in the course of ibuprofen release.Compared with polyester-amide (PIGA), the release rate of ibuprofen from polyester (PIGB and PIGH) chain was much slower.For the two polyesters, the ibuprofen release from PIGH is slightly slower than that from PIGB over the same time.Overall, the release rate of ibuprofen from PIGA was the highest and the release rate of ibuprofen from PIGH was the lowest.
The release of ibuprofen molecules was accomplished by the hydrolysis of the amide between ibuprofen and polymer main-chains as shown in Figure 1.The hydrolysis rate is inevitably dependent on the hydrophilicity of the three polymers as PIGA, PIGB, and PIGH carriers suspended in PBS.If the polymer carrier has higher hydrophilicity, water molecule is more conducive to reaction with amide bond, leading to the release of ibuprofen.It is well known that the contact angle is considered as an important reference for hydrophilicity/hydrophobicity of materials.The more hydrophilic the material is, the smaller its contact angle with water is.As shown in Figure 4(b), the contact angles of PIGA, PIGB, and PIGH were measured as 44 ∘ , 70 ∘ , and 82 ∘ , respectively.PIGA is the most hydrophilic, PIGB is more hydrophilic, and PIGH was the most hydrophobic.As shown from the results in Figures 5 and 6, the release rate of ibuprofen keeps in accordance with the hydrophilicity of PIGA, PIGB, and PIGH carrier.Therefore, the design and preparation of these polymer carriers also provide a possible method for regulating the release behavior of drug by changing hydrophilicity.

Cytotoxicity Evaluation for PIGB, PIGH, and PIGA.
In order to verify the potential application of PIGB, PIGH, and PIGA, the cytotoxicity of these polymer carriers was determined by MTT (CCK-8) method.HeLa cell used in this study was a common cell in the toxicity evaluation of new biomaterials, and the concentrations of PIGA, PIGB, and PIGH solutions were 0.01, 0.1, 1.0, 10, and 100 g/mL, respectively.After 48 h incubation, the viability of HeLa cells to PIGB, PIGH, and PIGA carriers reached up to more than 100% as shown in Figure 7. Besides, HeLa cells showed a normal cell proliferation even as the concentration of polymer solutions varied from 0.01 g/mL to 100 g/mL.These data indicate that PIGB, PIGH, and PIGA with ibuprofen as pendant group are mostly cytocompatible in the tested concentration range.

Conclusion
In this work, we described the synthesis and characterization of new biodegradable polyester and polyester-amide comprising biocompatible segments, with ibuprofen pendant group.Higher loading capacities of ibuprofen are achieved at the amount of 37.7% for PIGB, 35.2% for PIGH, and 35.3% for PIGA, respectively.The released ibuprofen preserves its chemical structure and its release behavior shows a duration mode with no burst.For polyester-amide PIGA, the continuous release of ibuprofen was more than 65% over 15 h in the mimicking physiological conditions, while the release of ibuprofen is only 21∼32% over 10 days from polyesters PIGH and PIGB.The release rate of ibuprofen is facilely regulated by the different hydrophilicity of PIGA, PIGH, and PIGB carriers.Moreover, PIGA, PIGH, and PIGB show no toxicity to HeLa cells by MTT (CCK-8) assay and the viability cells over 48 h are much higher than 100%.In brief, the preparation and release behaviors of three polymer carriers provide a reference for its application in drug release, especially in  treating inflammatory diseases and lowering the side-effects of conventional drug.

Figure 7 :
Figure 7: Viability of HeLa cells in different polymers solution over 48 h incubation.