Microparticles from Wheat-Gluten Proteins Soluble in Ethanol by Nanoprecipitation : Preparation , Characterization , and Their Study as a Prolonged-Release Fertilizer

At present, the development of natural polymeric microparticles is carried out to obtain release systems. Prolonged-release systems are a potential solution to avoid nitrogen (N) losses in agricultural fields. The aim of this study was to develop microspheres from wheat-gluten proteins soluble in ethanol 70% (v/v), to ascertain their characterization, and to study their potential application in agricultural fields. Soluble-protein extraction was performed with 1600mL of ethanol 70% (v/v). Likewise, ethanolic solutions with protein concentrations of 0.5%, 1%, and 2% (w/v) are classified as non-Newtonian fluids with pseudoplastic behavior. Using the nanoprecipitation method, it was possible to develop urea-loaded microspheres with a diameter ranging from 900 nm–1.7 μm. The Fourier transform infrared spectroscopy (FTIR) test exhibited interaction through hydrogen bonds between carbonyls and amino groups from the urea and proteins. Also, the thermogravimetric analysis (TGA) test demonstrated thermal stability at 130°C. The release experiment showed that the microspheres achieved equilibrium when 88% of the urea was released. Finally, according to the empirical model of Ritger and Peppas, urea release is carried out through Fickian diffusion. We conclude that the microspheres could be applied in the fields and with this improve agricultural practices. Also, they could reduce the potential environmental pollution and developing a sustainable agriculture.


Introduction
In recent years, the world population has increased, and also, its food requirement.Agriculture has been carried out since the early days of humans and comprises a primary food source for the latter.Therefore, better agronomic practices are required to meet the needs of the world population [1,2].At present, new technologies are studied for possible application in agri-food industries in order to achieve improvements in safety and health and to obtain better quality products, as well as to increase the yield of products from agricultural fields [2].
Microtechnology is a science that provides favorable qualities for application in this industry, and it has been implemented in other areas.Additionally, it provides the necessary tools that can be applied as an alternative for control and food production [3,4].The advantage lies in that microparticles can encapsulate active compounds that help to improve agricultural products.The nanoprecipitation method is easy, fast, and one with a low energy expenditure, and it has good results for the encapsulation of active compounds [5].The technique involves the formation of an organic phase composed of a polymer, an active compound, and the suitable solvent for both.This phase is added to an antisolvent phase, which gives rise to solvent displacement through the action of the compound's polarity.The process involves the nucleation process and concludes with the formation of nano-or microparticles [6,7].
In recent years, synthetic and natural polymers have been employed for the preparation of nano-and microparticles [8].Proteins are natural polymers utilized for the preparation of materials that are biodegradable and biocompatible [9].Wheat gluten is rich in gliadins and glutenins.Gliadins are monomeric proteins with a molecular weight within the range of 30,000-80,000 Da, and they are extracted from wheat gluten due to their solubility in alcoholic solutions.On the other hand, glutenins are polymeric proteins with a molecular weight falling with the range of 80,000 Da to more 10 million Da, and they are soluble in acidic solutions [10][11][12].Both proteins have been investigated for their use in the preparation of materials on the nano-and microscale, as well as in controlled-and prolonged-release systems, because of its application in agricultural fields and the food industry [13,14].
The application of pesticides and fertilizers is a necessary and common practice in agricultural fields.Fertilizers are rich in nutrients such as nitrogen (N), potassium (K), and phosphorus (P).Urea is one of the main fertilizers utilized worldwide because it is necessary for plant growth, due to its high N content (46%).Usually, urea is lost by means of the leaching, volatilization, and denitrification processes.This leads to obtaining low yields and low-quality products and could also produce environmental pollution [15,16].Therefore, nanoand micromaterials could be applied as the prolonged-or controlled-release systems of fertilizers in order to improve agricultural crops, increase product quality and yield, and decrease environmental pollution [13].The Agrifood and Fisheries Information Service (SIAP, its Spanish-language acronym) reports that Mexico produces about 3,710,706 tons of wheat per year, and 44% is produced in the northern state of Sonora, especially wheat of the species Triticum durum (SIAP, 2015 (https://www.gob.mx/siap)).
The aim of this work was the preparation and characterization of microparticles from Wheat-Gluten Proteins Soluble in Ethanol (WGPSE) (mainly gliadins).These microparticles were loaded with urea, and we conducted its prolongedrelease study.The latter was performed to obtain information to determine their potential use as urea prolonged-release systems and to attempt to apply better agronomic practices.

Production of Wheat Flour and Obtaining Wheat Gluten.
Wheat grain was conditioned with 16.5% moisture, and the flour was made using a mill (Brabender Quadrumat Senior), according to the 26-10 method (AACC, 2000).Wheat gluten was obtained when the flour was washed with distilled water several times in order to remove the starch.Yields of wheat flour and gluten were determined.
2.2.Determination of Protein Content.The analyses were determined through the Nitrogen/Protein Analyzer (LECO, Model NP528) using a conversion factor of 5.7.

Percentage of Solubility of Ethanol-Soluble Proteins from
Wheat Gluten.Percentage of solubility was calculated using the methods reported by Kayserilioğlu et al. [17] and Shukry et al. [18].In this experiment, 1 g of material previously extracted for this test was added to 20 mL of the solvent at different concentrations (ethanol/water mixture of 50, 60, 70, 80, 90, and 100% (v/v)).The suspensions were centrifuged at 8300g during 20 min (Hermle Labortechnik, Model z36HK), and the insoluble fraction was isolated, freeze-dried, and weighed.

pH Measurements.
The pH value of the solutions was determined using a pH meter HI-2550 (Hanna Instruments).

Standardization of the Extraction of Soluble Proteins in
Ethanol from Wheat Gluten.Proteins were extracted from wheat gluten using the method reported by Ang et al. [19] with some modifications in solvent volumes.100 g of gluten was added to different volumes of ethanol 70% (v/v) (Table 1).
The mixture was subjected to 12 h of propeller stirrer (Yamato Scientific, Model LT400D) and centrifuged at 3470g for 15 min.The supernatant was recovered and purified by dialysis process.The soluble fraction was freezedried (LABCONCO, Kansas City, MO, USA) and weighed.2.7.Rheological Characterization.Soluble-fraction solutions with concentrations of 0.5%, 1%, and 2% were prepared in ethanol 70% (v/v).Rheological measurements were carried out under the conditions reported by Sun et al. [20] utilizing a rheometer (Anton Paar Model MCR102) with a concentriccylinder system.The shear rate employed ranged from 0.1 s −1 to 1000 s −1 at 25 °C.
2.8.Statistical Analysis.Descriptive statistics were applied to the data obtained from this experiment, such as averages and standard deviations (SD).A Tukey mean-difference test was performed with a significance level of p > 0 05.

Development of Microparticles of Ethanol-Soluble
Proteins from Urea-Loaded Wheat Gluten.The microparticles were prepared by the nanoprecipitation method reported by Fessi et al. [21] with some modifications.The organic phase was made with ethanol 70% (v/v), mixing soluble proteins and urea and using concentrations of 1% (w/v) and 0.5% (w/v), respectively.The aqueous phase was prepared with distilled water and 0.05% (w/v) of Poloxamer 188.The organic phase was injected drop-wise into the aqueous phase 2.13.Urea-Release Experiment.The spheres were dispersed in a glass with 1 L of deionized water at 25 °C under stirring at 250 rpm utilizing a magnetic stirrer (IKA C-MAG HS7).The urea released was determined at 2, 4, 6, 8, 10, 12, 14, and 16 hours.Ten milliliters of sample was removed from the deionized water and replaced with 10 mL of deionized water in order to maintain 1 L in the aqueous medium, according to the methodology reported by Gulfam et al. [22].The urea concentration released into the aqueous medium was determined through the Berthelot method according to the Randox kit, employing UV-vis spectrophotometry using an equipment VARIAN model Cary 50.Reagent 1 (R1) was prepared adding R1a, which is formed by the urease enzyme, and R1b which is formed by the phosphate buffer (sodium salicylate, sodium nitroprusside, and EDTA).Then, three vials were prepared; (1) 1000 μL of R1 was added to form the reagent blank, (2) 10 μL of the standard and 1000 μL of the R1 were added and mixed to form the standard sample, and (3) 10 μL of the sample and 1000 μL of R1 were added and mixed.Then, the three samples were incubated during 5 minutes at 25 °C.After, 200 μL of sodium hypochlorite (R2) was added to each vial.The three samples were mixed separately and incubated during 10 min at 25 °C.Finally, the absorbance of the samples was measured at 600 nm.Berthelot method is based on the reaction of aqueous urea in the presence of the urease enzyme to transform into 2NH 3 + CO 2 .After, the salicylate and hypochlorite reagents react with the ammonium ions and transform the sample in a green complex named 2,2-dicarboxylindophenol.

Production of Wheat Flour and the Wheat Gluten
Obtained.The yield of wheat flour was 54% and the rest was wheat bran, and the wheat-gluten yield obtained was 9% (on a dry matter (DM) basis).Barak et al. [23] reported a wheat-flour yield of 65%, and these authors obtained wheat gluten with a range of 7.92-10% of protein, and wheat flour was obtained from four different varieties of wheat.The difference between both yields could be due to that the raw material for the experiments was different; this means that 11% less wheat flour was utilized for the combination of different wheat varieties of bread wheat and wheat pasta, while a wheat-flour yield of 54% was from wheat pasta only, which could be a factor in terms of the reduced yield in comparison with that of bread wheat.Moreover, values of wheat-gluten protein were similar between both studies.

Percentages of Solubility of Wheat-Gluten Proteins
Soluble in Ethanol (WGPSE).Table 2 presents the concentrations of ethanol tested and the pH of each.The high solubility in ethanol 70% (v/v) in comparison with that of the other concentrations is attributed to hydrogen bonds between the solvent and the protein.Soluble proteins are unfolded and possess the amino groups available for the interaction through hydrogen bonds.The isoelectric point of these proteins is around pH 7.8, considering mainly gliadins, and the remainder, low molecular weight (LMW) glutenins.The pH value of ethanol 70% (v/v) was 5.88 in comparison with the other pH values of the concentrations.It is remarkable that another factor that exerts an influence on the solution of ethanol 70% (v/v) in order for it to present over 90% solubility is that the pH value is far from the pH value for considering the isoelectric point.Joye et al. [24] report that the isoelectric point of gliadin protein is pH ≈ 7.8.Proteins do not reach the neutral charge of their amino acids; thus, they cannot reach their isoelectric point.This causes the protein to lose solubility due to the conformation that it adopts, in which its R side chains are accommodated in such a way that the solvent can interact with side chains that are as polar, such as ethanol 70% (v/v).This, in turn, causes a variation in its pH, which is reflected in its remoteness from its isoelectric point; therefore, its low solubility in the solvent.

Process of Obtaining a WGPSE.
The best amount of ethanol at 70% (v/v) to obtain WGPSE was 1600 mL, obtaining 34.96 g of proteins (Table 1).These results demonstrate that the volume at which ethanol 70% (v/v) can extract the greatest amount of gliadins and LMW glutenins from wheat gluten is by employing 1600 mL.This can be explained because the amount of ethanol is sufficient to interact with the majority of the gliadins and LMW glutenins, exhibiting easy interaction between the solvent and the protein structure.The ethanol-soluble protein fraction is composed mainly of LMW gliadins and glutenins (20 kDa-45 kDa) 3 International Journal of Polymer Science [25].The appropriate volume for best extraction of soluble proteins in ethanol was 1.6 L. However, as the ethanol volume increased, the yield decreased.This can be attributed to that ethanol interacts with all of the gliadins present in the solution.While the ethanol volume was increasing, the solvent began to separate LMW glutenins from the gliadins, i.e., excess of ethanol causes a dissociation of LMW glutenins, which are very similar to the gliadins, the reason why the yield of soluble proteins in ethanol decreases (Figure 1).Gliadins and glutenins are proteins classified as prolamins, which interact with each other because of the ease of their amino acids to form hydrogen bonding and disulfide-bond interactions [10,25,26].They mainly contain the amino acids glutamine, proline, methionine, isoleucine, cysteine, serine, phenylalanine, and tyrosine [10,25].
Wheat-gluten protein content was 77% (DM basis), and moisture content was 4.72%.The yield obtained was 34.92% (DM basis), with a protein content of 85% (DM basis) and a moisture content of 6%.Fajardo et al. [27] extracted gliadins from commercial wheat gluten, obtaining a gliadin-rich fraction equivalent to 15%, while this experiment demonstrates a higher protein yield due the quality of the extraction and focusing mainly on proteins soluble in ethanol, these mainly gliadins and LMW glutenins.The method was standardized, and this experiment revealed very good results, obtaining more than 34% of the gliadin-rich fraction.
3.4.Rheological Characterization. Figure 2 illustrates the effect of shear rate γ on shear stress τ for low concentrations at 25 °C.Protein solutions are classified as non-Newtonian fluid because they do not possess directly proportional behavior between shear stress and shear rate.Protein solutions do not follow Newton's law of viscosity.
The standard deviation (SD) of the average of each point of the experiment (protein solutions at 0.5%, 1%, and 2%) presents values near zero.Protein solutions exhibit apparent linear behavior with a shear rate ranging from 0 s −1 to 200 s −1 .Finally, experimental solutions with low concentrations of WGPSE comprise non-Newtonian fluid.At this point, protein solutions could entertain shear-thickening behavior.
Figure 3 depicts the effect of shear rate on viscosity for the three WGPSE concentrations at 25 °C, where it can be observed that the fluid is non-Newtonian, as in Figure 2 in terms of shear stress.In this case, when the shear rate increased, the apparent viscosity also increased.This is attributed to the energy-dissipation increase with the shear rate, resulting in shear-thickening (dilatant) behavior [28].
This result demonstrates that the protein solutions will continue to increase in viscosity with the nanoprecipitation technique's shear rate.Particle diameter could be affected by the type of fluid applied to the nanoprecipitation process, and it is possible that a non-Newtonian fluid with shearthickening behavior could develop particles with a size ranging in micrometers, because the viscosity will increase the nucleation rate.
Power law and Pearson correlation coefficient were applied to the results obtained from the solutions in order to confirm that protein solutions are non-Newtonian fluids and that they possess shear-thickening behavior, considering the flow-index value obtained and the R 2 value.Table 3 presents different concentrations of WGPSE and flow-index values.The flow index is above 1 at the three concentrations, implying that the protein solutions are non-Newtonian fluids with shear-thickening behavior.Concentrations of the protein solutions do not present significant differences in flowindex values.The solution with a concentration of 1% (w/v) is the suitable solution for obtaining a better yield in comparison with a protein solution of 0.5% (w/v) and of 2% (w/v).
Sun et al. [29] report that gliadin solutions in propanol 50% (v/v) are classified as Newtonian fluids, but gliadin solutions possess shear-thinning behavior (pseudoplastic behavior) when they maintain a pH of 2.0-8.Sun et al. [20] showed similar results to those of their later research, where the authors classified gliadin solutions as Newtonian fluids at different concentrations of the protein.The results obtained from this research reveal that protein solutions with low concentrations are non-Newtonian fluids with shearthickening behavior.When it is necessary to obtain particles with diameters in micrometers, it is recommendable to apply solutions with shear-thickening behavior.On the other hand, it is not recommendable to use this type of solution to obtain nano-or microscale particles because the increase of viscosity results in nonhomogeneous particles.The results of Sun et al. [20,29] are not similar to ours; first of all, proteins in our research were extracted from wheat gluten obtained from Triticum durum wheat grain, and it is important to consider the genetic differences between Triticum durum and Triticum aestivum.It is also important to bear in mind that, in the present study, proteins soluble in ethanol were tested.In addition, the solvent used for this experiment resulted as the best solvent in comparison with the others; propanol and ethanol yield different results in terms of protein solubility.In this experiment, ethanol was the best solvent for application in the nanoprecipitation process because it tends to form protein solutions, thus taking advantage of its high capacity to dissolve them, which is necessary to obtain particles through the nanoprecipitation technique.

Scanning Electron Microscopy (SEM) of Urea-Loaded
Wheat-Gluten Proteins Soluble with Ethanol (WGPSE) Microparticles and WGPSE without Urea.Figure 4 illustrates WGPSE microparticles; (a) without urea and (b) loaded with urea.Particle size ranges from 900 nm-1.7 μm with apparent spherical morphology; although agglomeration is present, but particle form and size are distinguishable, forming chains.Particle size between loaded and nonloaded microparticles does not have significant variation, and the urea is dispersed in the polymeric matrix; thus, it is not present in the micrographs.On the other hand, the stabilizer confers stability on the particle system between the polymeric matrix and the aqueous phase, but it was not 100% effective in this experiment.Therefore, there is agglomeration of the microparticles.On the other hand, the agglomeration afforded by noncovalent bonds does not exert an effect on the release, because the difference between the superficial area of particle in chain form and individual particles is not significantly affected.This information is in agreement with that mentioned by Bruinink et al. [30].These results are convenient for their potential application because it is important to maintain slow release of this fertilizer.Davidov-Pardo et al. [31] reported gliadin particles with spherical morphology with a diameter of 200 nm, and agglomeration is present but the particles are distinguishable.Hence, the results are similar in both studies; the important difference lies in particle diameter, which was provided by the rheological characteristics of the protein solutions subjected to the nanoprecipitation process.3.6.Fourier Transform Infrared Spectroscopy (FTIR).Figure 5 presents the FTIR spectra for wheat flour, wheat gluten, and WGPSE (gliadins, mainly).The FTIR of the wheat flour presents a medium-intensity band at 3296 cm −1 that corresponds to the stretching of the hydroxyl groups in the amino acids of the wheat-flour proteins.Likewise, a medium-intensity band at 2932 cm −1 corresponds to the stretching of the group -CH 2 -.Also, it presents a medium-intensity band at 1652 cm −1 and a weak-intensity band at 1535 cm −1 ; these latter bands correspond to the vibrational stretching of amide I (C=O) and the vibration frequency of amide II (N-H), respectively.Finally, there are strong-intensity bands at 1002 cm −1 , 1074 cm −1 , and 1150 cm −1 due to the vibrations of the -C-O-Cbonds present in the starch.The bands observed from 600 cm −1 to 1150 cm −1 are characteristic of the starch and are also called starch fingerprints due to their furanose and pyranose rings.The spectroscopy of wheat gluten and gliadins demonstrates a medium-intensity band at 3296 cm −1 and a medium-intensity band at 2932 cm −1 , and both bands were mentioned previously.Also present are the characteristic bands of amide I and amide II at 1655 cm −1 and 1536 cm −1 , respectively.The wheat-gluten spectrum does not exhibit the characteristic bands that represent the presence of starch.Due to the obtaining of wheat gluten, the starch content is removed.The spectrum corresponding to gliadins reveals the absence of the starch's characteristic bands, meaning that the production and purification processes were effective.Castro-Enríquez et al. [14] reported the presence of the characteristic bands in commercial wheat gluten, which are similar to those found in this paper for durum wheat gluten.Figure 6 presents the spectroscopy of (A) WGPSE (gliadins, mainly) and (B) WGPSE microparticles.WGPSE microparticles exhibit the bands of amide I and amide II.Likewise, we noted the band characteristic of the OH of the proteins.This spectroscopy demonstrates that gliadins do not have an alteration in their chemical structure after the nanoprecipitation process, due to that the latter is not an aggressive method and heat is not applied for the process to obtain nano-and microparticles.This means that the particles maintain the characteristic protein bands.The dominance of amide I bands shows that the protein maintains an alpha helix structure.This affirmation is in agreement with what was reported by Secundo and Guerrieri [32], where these authors mentioned the secondary structure of gliadins by FTIR spectrum.

Gliadin LMW glutenin
Figure 7 depicts the FTIR spectra of urea, gliadin microparticles without urea, and urea-loaded gliadin microparticles.Figure 7(a) presents the spectroscopy of urea, with a medium-intensity band at 3436 cm −1 , which corresponds to the primary amine characteristic in the urea.Also, it presents a medium-intensity band at 1678 cm −1 , which corresponds to the carbonyl group (C=O).Figure 7(b) demonstrates the spectroscopy of WGPSE microparticles without urea, revealing a low-intensity band at 3302 cm −1 and a mediumintensity band at 2880 cm −1 , corresponding to the stretching of the O-H group and the stretching of the C-H, respectively.
Figure 7(c) depicts the spectroscopy of WGPSE microparticles loaded with urea, with this spectroscopy presenting the strong-intensity band characteristic of the primary amine at 3436 cm −1 .In addition, it presents the strong-intensity band of the carbonyl group (C=O) found in urea and in WGPSE microparticles without urea.These results show that there is strong interaction afforded by the hydrogen bonds between the urea loaded and the gliadins (polymeric matrix), the latter performed by the amine group of the gliadins and the carbonyl group in the urea.This interaction is demonstrated by a shift-in-position of the carbonyl band to 52 cm −1 for urea and by a shift-in-position for gliadins to 29 cm −1 .

Thermogravimetric Analysis (TGA).
Figure 8 shows the TGA curves, and Table 4 indicates the weight loss percentage at different temperatures of WGPSE microparticles loaded with urea, WGPSE microparticles without urea, WGPSE (gliadins, mainly), and urea.Urea exhibits stability at around 145 °C and, at this point, begins its degradation, losing around 75% of its total weight when 228 °C is reached, subsequently completing its degradation at 477 °C.WGPSE microparticles show a weight loss of 6% when the temperature is around 100 °C; this loss is probably moisture.Also, from 100 °C to 218 °C, they exhibit a weight loss of around 8% of total weight, while after that, they present their most important weight loss, from 218 °C to 410 °C, when they lose 78% of weight, finally completing their degradation on reaching 800 °C.WGPSE microparticles without urea show an initial weight loss of about 2% when they are heated at 100 °C, which is probably moisture.When they reach 174 °C to 445 °C, they present a weight loss of 80%, while on reaching 800 °C, their total degradation is complete.These results demonstrate that microparticles without urea present stability at 174 °C, while WGPSE microparticles loaded with urea present a weight loss of 2% when reaching 130 °C, which is probably moisture.Finally, from 130 °C to 800 °C, their degradation is total.These results reveal that WGPSE microparticles loaded with urea can be used as release systems with high temperatures because they are stable at 130 °C.
3.8.Prolonged Release of Urea from WGPSE Microparticles.Figure 9 presents the results of the release of urea from WGPSE microparticles.WGPSE microparticles released around 50% of the urea during the first hour.This is attributed to that urea is found on the surface of the microparticles, which is in contact with the aqueous medium.Also, urea is present that is forming part of the polymeric matrix, due to that urea is a cross-linking compound.The urea present on the surface of the microparticles and the urea forming part of the polymeric matrix are the first urea that is in contact with the aqueous medium, resulting in the rapid release of the urea.These results demonstrated that WGPSE microparticles could be applied as prolonged-release fertilizer because the material presents a slow and gradual release until reaching equilibrium at 12 h.The experiment achieves equilibrium when the urea concentration inside of the microparticles is       International Journal of Polymer Science equal to the urea concentration in the aqueous medium (outside of the microparticles), releasing 88% of the total urea loaded, which occurred when the equilibrium of diffusion is reached [33].Castro-Enríquez et al. [14] developed urealoaded wheat-gluten membranes by means of the electrospinning technique to obtain a prolonged-release system, which achieves equilibrium at 5 hours.This result, in comparison with the results from this work, reveals an advantage of WGPSE microparticles in terms of release time.WGPSE microparticles exhibited a better release time for obtaining a prolonged-release system for potential applications in agricultural fields.
3.9.Release Kinetics of Urea.The release kinetics of urea was calculated using an empirical model developed by Ritger and Peppas.The data were modeled by (1) in its linearized form: in which M t is the amount of urea released at time t, M ∞ is the amount of urea released in an infinite time, k is the constant release kinetics, and n defines the mechanism of urea transport.On applying the empirical model of Ritger and Peppas, k = 0 63 and n = 0 08 were obtained.For this model, it is known that when n ≤ 0 45, the compound release mechanism is a Fickian diffusion, when n is between 0.5 and 1, the compound release mechanism is non-Fickian, and when n = 1, the release mechanism is anomalous (zero-order).
The results obtained using this model demonstrate that kinetics release is afforded by a Fickian diffusion mechanism.
The fact that the release mechanism is a Fickian diffusion provides an idea that when the WGPSE microparticles were applied, the concentration gradient exerts an effect to produce equilibrium in the system, executed by the diffusion process.The diffusion process is performed by the concentration gradient, and it takes advantage of the water solubility of urea in order to move it outside of the microparticles until an equal concentration is reached on both sides.This knowledge applied to agricultural fields comprises an advantage due to that the fields' soil is not completely wet, i.e., the WGPSE microparticles are not in constant contact with the irrigation water.This explanation reveals that this release mechanism could become a more prolonged release in the agricultural fields.Castro-Enríquez et al. [14] developed wheat-gluten membranes loaded with urea with an application in agricultural fields.The authors found that the release mechanism was carried out by relaxation of the polymeric chains and then by diffusion, obtaining a value of k = 0 07.The difference between the values of k published by Castro-Enríquez et al. [14] is attributed to the material's geometry and the release mechanism, i.e., the spheres are smaller than the membranes and do not require relaxation of the polymeric chains in comparison with that of membranes as reported by the authors.Consequently, release is increased due to the lack of the relaxation of the polymeric chains.The release process is carried out by a Fickian diffusion, which indicates that the process is performed in a single step influenced by the concentration gradient.This would explain why the release rate was much faster, in that it was not influenced by two release mechanisms, as reported for the membranes.This, in the end, would influence the prolonged release of urea through the material; however, the latter would continue to be a suitable material for its implementation as a release system.

Conclusions
Solutions of WGPSE at low concentrations demonstrate that they can be used to develop microparticles using the nanoprecipitation technique due to its shear-thickening behavior.Moreover, physicochemical characterization indicates that this material can be employed as a urea prolonged-release system.We conclude that urea-loaded WGPSE microspheres can be employed in agricultural fields as an alternative to avoid the loss of N and decrease the risk of the environmental

Table 1 :
Standardization of the extraction of proteins soluble in ethanol 70% (v/v) from wheat gluten.

Table 2 :
Effect of the ethanol concentration on the solubility and pH of WGPSE solutions.

Table 3 :
Effect of the concentration of wheat-gluten proteins soluble in ethanol (WGPSE) on the flow index (n) calculated by power law and Pearson correlation coefficient values.