C-Reactive Protein as a Prognostic Indicator in COVID-19 Patients

While some biomolecules have been explored to identify potential biomarkers for the prognosis of COVID-19 patients, there is no reliable prognostic indicator of the disease progression and severity. We aimed to evaluate the ability of the C-reactive protein (CRP) to predict COVID-19 infection outcome. This retrospective study was conducted on 429 patients diagnosed with COVID-19 between March 30, 2020, and April 30, 2020. The study population was divided into severe (n = 175) and nonsevere cases (n = 254). Data on demographic characteristics, clinical features, and laboratory findings on admission were collected. The proportion of patients with increased CRP levels was significantly higher in severe cases than in nonsevere patients. Analysis of the receiver operating characteristic (ROC) curve found that CRP could be used as an independent factor in predicting the severity of COVID-19. Also, patients with CRP >64.75 mg/L were more likely to have severe complications. In conclusion, CRP serum levels can predict the severity and progression of illness in patients with COVID-19.


Introduction
Since December 2019, a new type of coronavirus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causing coronavirus disease 2019 (COVID-19), has been identified in China [1]. e COVID-19 pandemic then spread quickly around the world [2,3]. By March 30, 2021, 127,349,248 confirmed cases of COVID-19, including 2,787,593 deaths, were reported to the World Health Organization (WHO) [4]. e rapid spread of the SARS-CoV-2, rapid changes in clinical features, and increased mortality have become the world's biggest concern. Furthermore, there are no reliable prognostic indicators for predicting disease severity and progression. Recognizing markers of disease severity may thus profoundly help to detect at-risk patients. Recently, some studies have reported that C-reactive protein (CRP) levels can be used in the early diagnosis of pneumonia and that higher CRP levels were associated with severe pneumonia [5].
Hence, the current study aims to evaluate the correlation between CRP levels and disease progression to provide a reference for the clinical management of COVID-19 patients.

Study Design, Participants, and Definition.
e present retrospective study was approved by the Ethics Committee of Babol University of Medical Sciences (code: IR.MUBABOL.REC.1399.041). e three affiliated hospitals of Babol University of Medical Sciences have been designated to treat patients with COVID-19. A total of 429 adult cases were confirmed at these centers from March 30 to April 30, 2020. All patients with COVID-19 who enrolled in the recent study were diagnosed according to the WHO interim guidance for COVID-19 (6th edition) [6]. In other words, all patients with the physician-and laboratoryconfirmed (positive nasopharyngeal/throat swab specimens by reverse transcription-polymerase chain reaction (RT-PCR)) COVID-19 infection were included, while suspected cases with similar clinical symptoms were excluded. All cases were monitored using the clinical data collected until March 30, 2020. One of the following criteria was used to determine severe COVID-19 illness: respiratory rate ≥30 bpm, oxygen saturation ≤93%, arterial oxygen partial pressure (PaO 2 )/ oxygen concentration (FiO 2 ) ≤300 mm Hg, and intensive care unit (ICU) admission.

Data Collection.
Patient medical records were reviewed by an experienced team of clinicians of the Infectious Diseases and Tropical Medicine Research Center of Babol University of Medical Sciences. e data on epidemiological, clinical, laboratory, radiological findings, and outcomes were collected using a data collection checklist from electronic medical records. Moreover, recorded patient data, such as demographic characteristics, past medical history (PMH), underlying medical conditions, symptoms, and signs, were collected.

Statistical Analysis.
e statistical data were analyzed using SPSS version 16.0 (IBM, Chicago, IL, USA). Continuous and categorical variables were presented as median (IQR) and n (%), respectively. Mann-Whitney U-test and Student's t-test were used to compare continuous and categorical variables. e predictive value of the CRP was evaluated by measuring the area under the receiver operating characteristic curve (AUC). e optimal threshold value was obtained by calculating the Youden index. A multivariate Cox proportional risk model was used to determine predictive factors for disease risk.

Results
ere were 429 patients with COVID-19 in our study. Of these, 175 patients (40.8%) were assigned to the severe group, while 254 patients (59.2%) were allocated to the nonsevere group. e demographic and clinical characteristics of the patients are summarized in Table 1.
e mean age was 57.21 ± 16.18 years, with a range of 16-99 years. e average age was higher in the severe group than in the nonsevere group (P � 0.111). One hundred and eighty-six patients (43.4%) were female. e severity ratio for males was higher than for females, but this difference was not significant (P � 0.122). e median duration from illness onset to discharge was seven days. Overall, dyspnea (72.5%) was the most common initial symptom, followed by fever (61.3%) and dry cough (57.6%). However, there was no significant difference in the symptoms ratio between the two groups.

Discussion
In the present retrospective study, the clinical characteristics of severe COVID-19 patients were compared with those of nonsevere patients and analyzed the possible factors associated with disease progression and severity. Furthermore, the prognostic value of the CRP in the progression of COVID-19 cases has been revealed. e current study evaluated the association between CRP and COVID-19 infection, and the findings indicated that a patient with a CRP level >64.75 mg/L was more likely to develop the severe form of the disease. In other words, ROC analysis confirmed CRP as a valuable predictor of COVID-19 progression and severity.
In response to infections, the liver synthesizes significant quantities of acute-phase proteins (APPs), such as CRP [7,8]. is acute inflammatory protein is a highly sensitive biomarker for inflammation, tissue damage, and infection [9]. It has been shown that CRP levels are correlated with levels of inflammation [10]. CRP levels can promote phagocytosis and activate the complement system [11]. In other words, CRP binds to microorganisms and promotes their removal through phagocytosis [12].
Moreover, the serum CRP levels increase during inflammatory responses. As shown previously, this biomarker may be raised by viral or bacterial infections. It is important to note that CRP levels were significantly increased in bacterial infections than in viral infections [13]. e current study revealed significantly higher CRP levels in severe cases than in nonsevere patients suggesting that the CRP level may be a biomarker of disease severity and progression in patients with COVID-19. Liu et al. reported that more severe cases infected with COVID-19 expressed significantly higher CRP levels than nonsevere patients [14]. Qin et al. observed higher CRP levels in severe COVID-19 patients than in nonsevere cases, suggesting that this biomarker can be monitored to evaluate disease progression [15]. Sahu et al. performed a meta-analysis to assess CRP levels as a potential biomarker of the COVID-19 prognosis.
eir results indicated that CRP concentrations remain high in expired patients and could be a promising biomarker for assessing mortality [16]. Also, some studies showed that some frequent complications in severe and expired COVID-19 patients, such as shock, ARDS, acute kidney injury, and acute cardiac injury, were correlated with higher CRP levels [17].

Limitations and Future Suggestions
e current study encompasses a short sample size. us, it may lack generalizability. Furthermore, the retrospective nature of this study and the consequent missing clinical data was another limitation. erefore, subsequent clinical studies with larger sample sizes and multiple CRP level measurements, especially at different treatment times, should be performed to confirm our findings.

Conclusions
Our findings suggest that serum CRP levels could be used as an essential indicator of the progression and the severity of COVID-19. Also, the present study suggests that patients with higher CRP levels should be carefully monitored throughout their disease course.

Data Availability
All data are available upon reasonable request to the corresponding author.

Conflicts of Interest
e authors declare that they have no conflicts of interest. Interdisciplinary Perspectives on Infectious Diseases 5