Geographical Characterization of Tunisian Olive Tree Leaves (cv. Chemlali) Using HPLC-ESI-TOF and IT/MS Fingerprinting with Hierarchical Cluster Analysis

The olive plant has been extensively studied for its nutritional value, whereas its leaves have been specifically recognized as a processing by-product. Leaves are considered by-products of olive farming, representing a significant material arriving to the olive mill. They have been considered for centuries as an important herbal remedy in Mediterranean countries. Their beneficial properties are generally attributed to the presence of a range of phytochemicals such as secoiridoids, triterpenes, lignans, and flavonoids. With the aim to study the impact of geographical location on the phenolic compounds, Olea europaea leaves were handpicked from the Tunisian cultivar “Chemlali” from nine regions in the north, center, and south of Tunisia. The ground leaves were then extracted with methanol : water 80% (v/v) and analyzed by using high-performance liquid chromatography coupled to electrospray time of flight and ion trap mass spectrometry analyzers. A total of 38 compounds could be identified. Their contents showed significant variation among samples from different regions. Hierarchical cluster analysis was applied to highlight similarities in the phytochemical composition observed between the samples of different regions.


Introduction
Natural antioxidants present in the diet have evidenced the increase of the resistance toward oxidative damages, and they may have a substantial impact on human health [1]. Furthermore, the use of natural extracts from plants as functional ingredients in food, beverage, and cosmetic applications is receiving a great deal of attention among scientists, as well as among consumers and food manufacturers [2]. Recently, there has been a renewed interest in natural product research due to the failure of alternative drug discovery methods to deliver many lead compounds in key therapeutic areas such as immune suppression, antiinfectives, and metabolic diseases [3]. Despite competition from other drug discovery methods, natural products are still providing their fair share of new clinical candidates and drugs [3]. We can find several natural products as sources of new drugs that have been recently reviewed in literature [4][5][6][7]. e olive plant has been extensively studied for its nutritional value, whereas its leaves have been specifically recognized as a processing by-product [8]. Considered as byproducts of olive farming, olive tree leaves represent a significant material arriving to the olive mill [9]. ey have been considered for centuries as an important herbal remedy in Mediterranean countries. eir positive effects on human health are multiple: antioxidant [10][11][12][13][14][15][16], hypoglycemic [17], antimicrobial [18], and anticancerous [19][20][21][22] among others. ese properties are generally attributed to the presence of a range of compounds, such as secoiridoids, triterpenes, lignans, and flavonoids. It has been reported that byproducts can have a similar or even higher proportion of bioactive compounds than the usable parts of produce [23]. For instance, the characterization and quantification of bioactive compounds is one of the first steps to be taken in any evaluation of the putative contribution of the olive and its derivatives to human health [24] since the chemical composition of olive tree leaves varies depending on several conditions such as origin, proportion of branches on the tree, storage conditions, climatic conditions, moisture content, and degree of contamination with soil and oils [25].
Recently, the effects of abiotic and biotic factors on the phenolic composition in olive tree leaves have been reviewed in literature [26]. Biotic factors such as genotype, load bearing, leaves age, and fungi and bacteria attacks as well as abiotic factors such as hydric deficiency, salinity, fertilization, sampling time, geographical zone, and climatic conditions influence the contents of phenolic compounds [26]. Recent researches revealed the impact of the geographical location on the total phenolic composition of olive leaves [27][28][29][30]. However, the data on the variation of the individual phenolic compounds in olive leaves are scarce. According to our knowledge, this is the first study on the phenolic compounds in olive tree leaves from Tunisian cultivars according to the geographical region. Considering the importance of phenolic compounds in olive leaves, their determination and the scarce literature related to their behavior according to geographical origin, the aim of this study was to explore the phenolic composition of the leaves from the Tunisian olive cultivar "Chemlali" from different localities. is cultivar was chosen since it is widespread throughout Tunisia from the north to the south.

Sample Collection and Preparation of Extracts.
Fresh olive leaves from the Tunisian "Chemlali" cultivar were obtained at the time of olive pruning from different regions from Tunisia (north, center, and south from coastal and mountainous areas) (available here). Leaves were dried at 25°C, ground, and then 1 g of the obtained homogenized powder was dissolved in 10 mL of methanol : water 80% (v/v), and the mixture was allowed to stand in the dark for 24 h [31]. e extract was centrifuged at 5000 g for 10 min at room temperature, and the supernatants were then filtered using a 0.45 µm syringe filter to be analyzed by highperformance liquid chromatography (HPLC).

Chromatographic Separation of Phenolic
Compounds. An Agilent 1200 HPLC system (Agilent Technologies, Waldbronn, Germany) equipped with a vacuum degasser, autosampler, a binary pump, and a DAD detector was used for the chromatographic determination. Phenolic compounds were separated by using an Agilent Eclipse Plus C18-column (4.6 × 150 mm, 1.8 mm particle size) operating at 25°C and a flow rate of 0.8 mL/min. e mobile phases used were water with acetic acid (0.5%) (phase A) and acetonitrile (phase B), and the solvent gradient changed according to the following conditions: 0 to 10 min, 5-30% B; 10 to 12 min, 30-33% B; 12 to 17 min, 33-38% B; 17 to 20 min, 38-50% B; and 20 to 23 min, 50-95% B. Finally, the B content was decreased to the initial conditions (5%) in 2 min, and the column reequilibrated for 10 min. A volume of 10 mL of the extracts was injected. e separated compounds were monitored in sequence first with a diode-array detector (DAD) at 240 and 280 nm and then with a mass spectrometry (MS) detector.

Time of Flight and Ion Trap Mass Spectrometry
Detection.
e HPLC system was firstly coupled to a Bruker Daltonics micrOTOF mass spectrometer (Bruker Daltonics, Bremen, Germany) using an orthogonal electrospray interface. e mass spectrometer was operated in the negative ionization mode and acquired data in the mass range from m/z 50 to 1000 with a spectra rate of 1 Hz. e capillary was set at +4 kV, the end plate offset at −500 V, the nebulizer gas  at 2 bar, and the dry gas at 9 L/min at 250°C. e accurate mass data of the molecular ions were processed through the software data analysis and target analysis 4.0 (Bruker Daltonics). External mass spectrometer calibration was performed using a Cole-Parmer syringe pump (Vernon Hills, Illinois, USA) directly connected to the interface, equipped with a Hamilton syringe (Reno, Nevada, USA). e standard solution was injected at the beginning of the run, and all the spectra were calibrated prior to phenolic compounds' identification. en, the same HPLC system was coupled to a Bruker Daltonics Esquire 2000 ion trap mass spectrometer (Bruker Daltonics, Bremen, Germany) equipped with an electrospray interface (Agilent Technologies, CA, USA) operating in the negative ionization mode. e ion trap scanned at the 50-1000 m/z range at 13000 µ/s during the separation and detection. e maximum accumulation time for the ion trap was set at 200 ms, the target count at 20000, and compound stability was set at 50%. e optimum values of the ESI-MS parameters were as follows: capillary voltage, +3.0 kV; drying gas temperature, 300°C; drying gas flow, 7.0 L/min; and nebulizing gas pressure, 21.7 psi. e instrument was controlled by Esquire NT software from Bruker Daltonics. Peak identification was performed on the basis of their relative retention time values, TOF-MS and IT-MS/MS data, comparison with authentic standard solutions, and using the information previously reported in the literature [32]. Limits of detection (LOD) and quantification (LOQ) were respectively as follow: 0.09 and 0.3 ppm for hydroxytyrosol, 0.31 and 1.03 ppm for tyrosol, 0.02 and 0.06 ppm for luteolin, and 0.02 and 0.06 ppm for apigenin.

Radical-Scavenging Capacity (RSC).
e olive leaf samples were analyzed for their capacity to scavenge the stable DPPH • radical [33]. A volume of 3 mL of a freshly prepared DPPH solution (10 −4 M in methanol) was added to 0.5 mL of

HPLC-MS Analysis of the Olive Leaf
Extract. e base peak chromatogram (BPC) of "Chemlali" olive leaf extract and the compounds characterized in the negative ion mode are shown in Figure 1. Peak identification was carried out using Target Analysis software (Bruker Daltonics), by comparing both migration time and accurate MS and MS/MS spectral data obtained from olive leaf samples and commercial standards, together with the information previously reported in the literature. Table 1 summarizes the information about the identified phenolic compounds, in "Chemlali" olive leaf extracts, together with their corresponding retention time, theoretical mass to charge ratio (m/z), molecular formula, and IT/MS 2 fragments. A total of 38 compounds could be identified. ree main phenolic groups could be clearly distinguished: phenolic alcohols (hydroxytyrosol, tyrosol, and tyrosol glucoside), flavonoids (luteolin rutinoside, 2 isomers of luteolin glucoside, 2 isomers of diosmin, apigenin rutinoside, apigenin glucoside, chryseriol glucoside, luteolin, apigenin, rutin, quercetin, gallocatechin, taxifolin, and diosmetin), and various secoiridoids (particularly, oleuropein with 3 isomers, methoxyoleuropein, ligstroside, oleuropein aglycon derivative and related compounds such as elenolic acid glucoside isomers, and secologanoside). Apart from them, other polar compounds were also found; they are quinic acid, caffeoylquinic acid isomers, and benzyl alcohol hexose-pentose. A certain tendency in the elution order of the compounds related to their chemical structure class was observed, appearing in the following order     of increasing retention time (RT), and thus hydrophobicity: simple phenols, secoiridoids and related compounds, and flavonoids ( Figure 2). As can be observed in Table 2, some qualitative differences were registered among olive leaf samples from the geographical studied regions. Quantitatively, significant differences were found in a wide number of phenolic   compounds according to the geographical origin of the samples. Our results are in agreement with those reported in literature. In deed comparing Tunisian cultivars located in southern and northern Tunisia, significant differences were observed for phenolic composition as well as antioxidant activity where higher levels were registered for samples from the north [29].
Bilgin and Şahin [28] demonstrated that total phenolic content and extract yield varied significantly among Turkish olive cultivars from six sites in Anatolia. e authors observed that the total phenolic contents in olive leaves decreased as the geographical altitude decreases [28]. In another study on Greek olive cultivars, leaves collected from three different locations showed differences in total phenolic contents and antioxidant activity [27]. Similarly, the geographical zone has influenced the phenolic composition of Arbequina cultivar samples collected from six regions in Spain [30]. Using hierarchical cluster analysis, the latter study showed high similarities between the olive leaf samples from Cordoba and Navarra and both samples from Mallorca, respectively.
In our study, significant differences were observed for total phenolic contents in "Chemlali" olive leaf extracts from different regions (Figure 3). ese differences could be explained by the most marked variation that was observed for flavonoids and secoiridoids and related compounds as well as for phenolic alcohols classes especially for the leaves collected from Lamta region. ese classes presented amounts that reached 18, 78 and 15% for Lamta, Jelma, and Sidi Bou Ali, respectively. Furthermore, we can observe that secoiridoids' tendency was the inverse of that of flavonoids ( Figure 4).
Being the major one among the identified compounds, oleuropein amounts varied significantly among olive leaf samples. e registered values were between 15.8 and 42.25% for samples from Gabes and Lamta regions, respectively.
Considering the subclasses of flavonoids, the most pronounced variation among samples from different regions was registered for flavones and flavanols. Flavones presented the highest percentage among the rest of flavonoids followed by flavanols. Among analyzed samples, olive leaves from  Gabes showed the highest percentage in flavones, whereas leaves from Lamta showed the highest levels in flavanols presented by gallocatechin (Figures 5 and 6). Radical-scavenging activity is very important, due to the deleterious role of free radicals in foods and in biological systems. is test is a standard assay in antioxidant activity studies and offers a rapid technique for screening the radicalscavenging activity of specific compounds. e stable free DPPH radical is a useful reagent to investigate the scavenger properties of phenolics [34]. e radical-scavenging capacity values varied significantly between studied samples: the leaves from Zaghouan presented the highest radicalscavenging capacity, whilst leaves from Teboulba showed a radical-scavenging capacity of 49% (Figure 7).

Chemometrics.
Data clustering is a common technique for statistical data analysis, which is used in many fields. It is known as the classification of objects into groups (clusters), so that the data in each subset share some common trait-often proximity according to some defined distance measure [35]. e hierarchical analysis is a kind of very important clustering methods. It is an unsupervised technique that examines the interpoint distances between all of the samples and represents that information in the form of a two dimensional plot called a hierarchical tree or dendrogram.
In our study, an overall cluster analysis based on the determined metabolites and radical-scavenging capacity was performed to study the general differences among samples. To display the similarities between olive leaf samples from different provenance, a dendrogram was produced by applying a hierarchical clustering algorithm to the data.
As can be seen in Figure 8, there is a clear separation among the clusters established for different samples. Applying Ward's method based on Euclidean distance, olive leaf samples could be gathered into five different groups with respect to their phenolic composition: the first presented by Gabes region; the second by Ghraiba, Kondar, Sidi Bou Ali, and Teboulba; and the third by El Hajeb. e fourth group is composed by Jelma and Zaghouan and the fifth by Lamta.
It is useful to mention that HCA could be successfully applied for the classification and evaluation of the regional growing effects on the specification of the olive leaves based on their phenolic profile. In Figure 9, the profiles of the distinguished clusters or groups are illustrated. As can be seen, the leaves from Lamta region located in the coastal center of Tunisia are richer in gallocatechin and oleuropein aglycone derivative. Groups 1 and 4 presented the leaves richer in oleuropein isomer 1. Moreover, leaves from Gabes (group 1) located in the coastal south of Tunisia showed their richness in oleuropein aglycone. ese results are important since they may be useful for further researches and give an insight into the behavior of phenolic compounds in olive leaves from different environments. Such compounds have shown to be sensible to the edaphoclimatic variation between the considered regions of cultivation of "Chemlali" cultivar.

Conflicts of Interest
e authors declare no conflicts of interest.