Journal of Analytical Methods in Chemistry
Volume 2019 (2019), Article ID 2837406, 9 pages
https://doi.org/10.1155/2019/2837406

Research Article

Simultaneous GC-FID Quantification of Main Components of Rosmarinus officinalis L. and Lavandula dentata Essential Oils in Polymeric Nanocapsules for Antioxidant Application

Perla Giovanna Silva-Flores,¹ Luis Alejandro Pérez-López,¹ Verónica Mayela Rivas-Galindo,¹ David Paniagua-Vega,1,2 Sergio Arturo Galindo-Rodríguez,³ and Rocío Álvarez-Román¹

¹Departamento de Química Analítica, Facultad de Medicina, Universidad Autónoma de Nuevo León, Av. Fco. I. Madero y Dr. E. Aguirre Pequeño S/N, 64460 Monterrey, Nuevo León, Mexico
²Cátedras CONACYT-UANL, Departamento de Química Analítica, Facultad de Medicina, Universidad Autónoma de Nuevo León, Av. Fco. I. Madero y Dr. E. Aguirre Pequeño S/N, 64460 Monterrey, Nuevo León, Mexico
³Departamento de Química Analítica, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Av. Pedro de Alba S/N, 66455 San Nicolás de los Garza, Nuevo León, Mexico

Correspondence should be addressed to Rocío Álvarez-Román; roc_alvarez_r@yahoo.com

Received 17 October 2018; Revised 21 December 2018; Accepted 10 January 2019; Published 10 February 2019

Academic Editor: Jaroon Jakmunee

Copyright © 2019 Perla Giovanna Silva-Flores et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

The essential oils (EO) of R. officinalis and L. dentata have been widely used due to their antioxidant activity. However, due to their high volatility, the loading of EO into polymeric nanocapsules (NC) represents an efficient way of retaining their effect in future topical administration. In this way, the quantitative determination of EO incorporated into NC is necessary for simultaneous monitoring of the main components of the EO during the nanoencapsulation process as well as for precise and exact dosing of the components used during the performance of in vitro and in vivo biological tests. In this study, EO were isolated by hydrodistillation in a Clevenger-type apparatus and characterized by GC-MS and GC-FID analyses. The major constituents of EO-R. officinalis were camphor (39.46%) and 1,8-cineole (14.63%), and for EO-L. dentata were 1,8-cineole (68.59%) and β-pinene (11.53%). A new analytical method based on GC-FID for quantification of free and encapsulated EO was developed and validated according to ICH. Linearity, limit of detection and quantification, and intra- and interday precision parameters were determined. The methods were linear and precise for the quantification of the main components of EO. The EO were encapsulated by nanoprecipitation and were analyzed by the GC-FID method validated for their direct quantification. The NC size was 200 nm with homogeneous size distribution. The quantification of the incorporated EO within a NC is an important step in NC characterization. In this way, an encapsulation efficiency of at least 59.03% and 41.15% of total EO-R. officinalis and EO-L. dentata, respectively, was obtained. Simple, repeatable, and reproducible methods were developed as an analytical tool for the simultaneous quantification of the main components of EO loaded in polymeric nanocapsules as well as their monitoring in biological assays.

1. Introduction

Essential oils (EO) have been used for years in pharmaceuticals, cosmetics, and food products due to their health benefits [1], as antimicrobials [2], antidiabetics [3], analgesics [4], antioxidants [5], anti-inflammatories [6], and sedatives and anxiolytics [7]. EO are complex mixtures of volatile compounds that contain about 20 to 60 components at different concentrations. In general, it has been found that the main components could determine the biological properties of EO in living beings [1, 8]. In this sense, it has been reported that the EO of Rosmarinus officinalis (EO-R. officinalis) has antioxidant [9, 10], antimicrobial [11], anti-inflammatory, and antinociceptive [12, 13] properties, mainly due to the presence of monoterpenes such as 1,8-cineol, camphor, and α-pinene [9]. Similarly, the EO of Lavandula dentata (EO-L. dentata), belonging to the same family Lamiaceae, have been widely used because of their pharmacological effects as an anticonvulsant and antidepressant [14], sedative [15], and antioxidant [16]. These biological activities are attributed to the presence of monoterpenes such as linalool, camphor, and 1,8-cineol [17].

However, EO are highly volatile and can decompose easily due to direct exposure to heat, moisture, light, or oxygen [18]. Nanoencapsulation represents a feasible and efficient alternative to overcome these drawbacks since it would provide stability to the active substance [19], protection against oxidation [20], retention of volatile compounds, and reduction of side effects [20, 21]. In recent years, there has been growing interest in the development of polymeric nanoparticles, particularly nanocapsules (NC), as systems for the administration of lipophilic bioactive components [22], representing a new form of topical administration of EO with a preventive and/or therapeutic antioxidant effect. In this study, Eudragit EPO was used as the NC-formed polymer due to its cationic nature which will allow a greater interaction with the skin, as a result of the electrostatic interaction [23].

On the other hand, the chemical characterization and the quantitative determination of EO incorporated in NC arise as a need for the simultaneous monitoring of the main components of the EO during the nanoencapsulation process and the precise and exact dosing of the components used during the performance of in vitro and in vivo biological tests.

In this sense, the quantification of EO by HPLC has been limited by the high volatility and low UV absorptivity that most of these components have, presenting a greater application in the analysis of its nonvolatile components or other natural products (i.e., extracts) [24, 25]. In contrast, gas chromatography with mass spectrometry (GC-MS) has been used, given the ionization properties of the volatile components of the EO; however, it is an analytical technique that presents difficulties in the identification of the signals of these complex samples due to the fact that many terpenes have identical mass spectra as a consequence of the close similarities in fragmentation patterns and rearrangements after ionization, in addition to having a prolonged analysis time and high cost. [26–28].

Gas chromatography with a flame ionization detector (GC-FID) represents an analytical technique suitable for the qualitative and quantitative analysis of EO since it offers high sensitivity, great stability, and an exceptionally high linear dynamic range that allows the analysis of volatile components of the EO at very low concentrations or at trace levels [29, 30]. To date, there is no analytical method validated by GC-FID by direct injection whose applicability is the simultaneous and quick quantification of essential oil components in polymeric NC.

The main objective of this work was to develop and validate an analytical method by GC-FID by direct injection for the simultaneous quantification of the main monoterpenes of free and nanoencapsulated EO of R. officinalis and L. dentata for future application as an antioxidant agent in biological assays.

2. Material and Methods

2.1. Plant Materials and Reagents

R. officinalis and L. dentata were collected from Monterrey, N. L., Mexico. The specimens were identified in the Herbarium of the School of Biological Sciences, Universidad Autónoma de Nuevo Leon, Mexico. Dichloromethane (Baker, USA), methanol (Tedia, USA), acetone (CTR Scientific, USA), and isopropyl alcohol (Chromadex, USA) were of HPLC grade. Purified water was from a Milli-Q water-purification system (Veolia, USA). 1,8-cineol (>98%), β-pinene (98.5%), camphor (>95%), 4-allylanisole (98%), and linalool (>95%) were of GC grade (Aldrich, USA). The standard solution was n-alkanes (C8-C24, Sigma-Aldrich, USA). Eudragit EPO, an NC-formed polymer, was obtained from Evonik Industries, Germany.

2.2. Essential Oil Extraction

The essential oils were isolated from the aerial parts of fresh plants of R. officinalis (EO-R. officinalis) and L. dentata (EO-L. dentata) by hydrodistillation using a Clevenger-type apparatus for 4 h. The essential oils obtained were stored in a sealed vial at −4° C until chromatographic analysis. The yield percentage was calculated as weight (g) of essential oils per 100 g of the plant.

2.3. Essential Oil Analysis

2.3.1. Gas Chromatography-Mass Spectrometry (GC-MS)

The composition of volatile constituents of the essential oil was analyzed using a gas chromatograph 6890N (Agilent Technologies, USA) equipped with a 5973 INERT mass selective spectrometer (ionization energy 70 eV) and an HP-5MS column (30 m × 0.25 mm × 0.25 µm). Helium (99.99%) was the carrier gas at a flow rate of 0.5 mL min⁻¹. Data acquisition was performed in the scan mode. The ionization source temperature was 230°C, quadrupole temperature was 150°C, and injector temperature was 220°C. The oven temperature was programmed as follows: 35°C for 9 min, increased from 35°C to 150°C at 3°C min⁻¹, held at 150°C for 10 min, increased to 250°C at 10°C min⁻¹, increased at 3°C min⁻¹ to 270°C, and held at 270°C for 10 min. The samples were injected as mentioned above. Components were identified by comparing retention indices relative to C8–C20 n-alkanes, and the mass spectra were compared with the mass spectra from the US National Institute of Standards and Technology (NIST) library and the reference data [31].

2.3.2. Gas Chromatography with Flame Ionization Detection (GC-FID)

The GC-FID analysis of the EO was performed with a gas chromatograph Clarus 480 (Perkin Elmer, USA) equipped with a flame ionization detector and an Elite-5 capillary column (30 m × 0.25 mm × 0.25 μm; PerkinElmer, USA). The oven temperature was programmed as mentioned above. The detector and injector temperatures were 280°C and 220°C, respectively. The carrier gas was helium (99.99%) at a flow rate of 0.5 mL min⁻¹. Essential oil samples (2 μL) were injected using the split mode. The percentage composition of EO was calculated using the peak normalization method.

2.4. Preparation of Standard Solutions

Standard solutions were prepared by dilution of a stock solution (50 mg·mL⁻¹) prepared from the two main components of each essential oil in the following concentrations: EO-R. officinalis, 1,8-cineol (20 mg·mL⁻¹) and camphor (30 mg·mL⁻¹) and EO-L. dentata, β-pinene (7 mg·mL⁻¹) and 1,8-cineol (43 mg·mL⁻¹).

The working solutions were prepared in methanol in a concentration working interval using linalool (100 µg·mL⁻¹) and 4-allylanisol (100 µg·mL⁻¹) as internal standards for the EO-R. officinalis and EO-L. dentata, respectively. Standards were stored at −4°C and protected from light until use.

2.5. Validation Method by GC-FID

The quantitative analysis of the main components used as standard solutions and present in nanocapsules (NC) was performed by GC-FID by direct injection using a gas chromatograph Clarus 480 (Perkin Elmer, USA) equipped with a flame ionization detector and an Elite-5 capillary column (30 m × 0.32 mm i.d. 0.25 μm film thickness; PerkinElmer, USA). For the R. officinalis essential oil, the oven temperature was held at 60°C for 1 min, increased at 10°C min⁻¹ to 160°C, increased at 20°C min⁻¹ to 200°C, and finally increased at 15°C min⁻¹ to 230°C (and held) for 1 min. While for the L. dentata essential oil, the oven temperature was held at 70°C for 1 min, increased at 8°C min⁻¹ to 130°C (and held) for 1 min, increased at 17°C min⁻¹ to 170°C, and finally increased at 25°C min⁻¹ to 230°C. The detector and injector temperatures were 280°C and 220°C, respectively. The carrier gas was helium (99.99%) at a flow rate of 1.0 mL·min⁻¹. The injection volume was 2 μL using the split mode.

2.6. Method Validation

The method was validated in terms of linearity, limit of detection (LOD), limit of quantification (LOQ), and accuracy and precision (intra- and interday) according to International Conference on Harmonisation guidelines [32].

The linearity was determined by calculating a regression line from the plot of the peak area versus concentration in a range from 10 to 160 μg·mL⁻¹ in triplicates. The linearity was evaluated by calculation of a regression line using the least squares method.

The LOD and LOQ were calculated from the calibration curve according to

where is the standard deviation of the response and is the slope of the calibration curve. The residual standard deviation of the line regression or the standard deviation of -intercepts of the line regression may be used as the standard deviation.

Accuracy was determined by calculating the recovery percentage using the standard addition method. The determination of the concentration of 1,8-cineol and camphor in the EO-R. officinalis and β-pinene and 1,8-cineol in the EO-L. dentata was performed by analyzing the NC loaded with EO added with five levels of total concentration of standards (range 10–160 μg·mL⁻¹) in triplicates. The average concentration value obtained for each level was compared with the theoretical value, which was considered to be 100%, and the SD was determined.

Precision was evaluated in terms of repeatability (intraday precision) and intermediate precision (interday precision) and was expressed by percentage recovery and the SD for each main component.

Repeatability was determined by analyzing EO-loaded NC added with three levels of concentration (10, 40, and 160 μg·mL⁻¹) with six replicates each, during a single day. The intermediate precision was evaluated for repeatability during three different days.

2.7. Nanoencapsulation of the R. officinalis and L. dentata Essential Oils

EO-loaded NC were prepared according to the nanoprecipitation method developed by Fessi et al. [33]. The organic phase was prepared by dissolving 450 mg of Eudragit EPO® and 225 mg of EO-R. officinalis or EO-L. dentata in 15 mL of organic solvent mixture (acetone:isopropyl alcohol (50 : 50)) under magnetic stirring at room temperature. The organic solution was added to 20 mL of the aqueous phase (Milli-Q water) under moderate magnetic stirring (125 rpm). Finally, the organic solvent mixture was eliminated by dialysis. Briefly, the suspension was transferred to a membrane of regenerated cellulose Spectrum/Por (Spectrum Labs, USA), which was placed in a container with distilled water in agitation at 25°C during 2 hours with two changes of water. NC characterization was carried out to determine mean size, zeta potential, and polydispersity index (PDI) by dynamic light scattering (DLS) (Zetasizer Nano ZS 90, Malvern Instruments, UK) at 25°C. The stability of NC was determined from the particle size during 8 weeks (25 ± 2°C) in terms of means size and the PDI.

2.8. Method Applicability

2.8.1. Determination of Encapsulation Loading (% EL) and Encapsulation Efficiency (% EE)

Once the chromatographic methods were development and validated, the amount of EO-R. officinalis and L. dentata in the NC was determined indirectly. The NC dispersions were centrifuged at 25,000 rpm at 3°C for 2 h (Allegra 64R Centrifuge, Beckman Coulter, USA). The supernatant was removed and the pellet was washed thrice with methanol to ensure the complete solubilization of monoterpenes. Methanol solutions were analyzed by the previously validated GC-FID method in order to quantify the monoterpenes in NC. Finally, the %EL (3) and %EE (4) were derived as follows:

3. Results and Discussion

3.1. Extraction of the Essential Oils

In the present study, the EO were obtained by hydrodistillation with a yield percentage of 0.72 ± 0.19% (w/w) and 0.53 ± 0.29% (w/w) for R. officinalis and L. dentata, respectively. The yield of EO as well as their variations according to the origin of the plant has been reported in different investigations [34]. The yield obtained for the R. officinalis essential oil was higher than that reported by Bekkara et al., with a yield of 0.8% for the plant from Algeria [35], while the yield of lavender EO was lower than that reported by Imelouane et al., with a yield of 1.41% for L. dentata from Morocco [36]. This variability may be due to different collection times and/or climatic conditions at the plant-collection sites [37]. For example, Zaouali et al. affirmed that variations in the yield from different varieties of Rosmarinus EO from Tunisia could be attributed to variations in climatic conditions [10].

3.2. Chemical Composition of Essential Oils

Essential oils were analyzed by GC-MS and GC-FID to identify their constituents. In Figure 1(a) and 1(b), the GC-MS chromatograms of the EO of R. officinalis and L. dentata, respectively, are presented.

(a)

(b)

Figure 1: Chromatogram GC-MS of essential oils of R. officinalis (a) and L. dentata (b).

A total of 22 components of the R. officinalis essential oil were identified. The EO-R. officinalis composition was characterized by the presence of oxygenated monoterpenes (62.34%) represented mainly by camphor (39.46%) and 1,8-cineole (14.63%) (Figure 1(a)).

In L. dentata essential oil, 16 components were identified; the principal constituents were oxygenated monoterpenes (80.71%) and hydrogenated monoterpenes (16.19%); the main constituents were 1,8-cineole (68.59%) and β-pinene (11.53%) (Figure 1(b)).

The retention time, retention indices, and chemical composition of essential oils are presented in Table 1.

Table 1: Chemical composition percentage of essential oils (% relative to the peak area).

Composition
RIa
RIb
R. officinalis
L. dentata

Rt (min)
Peak area (%)
Rt (min)
Peak area (%)

Monoterpene hydrocarbons

    α-Pinene
934
939
18.54
9.43
18.48
2.87

    Camphene
949
954
19.38
4.97
—
—

    β-Pinene
977
979
21.13
4.75
21.17
11.53

    Myrcene
993
990
22.29
3.24
22.26
0.55

    α-Phellandrene
1003
1002
22.92
6.37
—
—

    α-Terpinene
1017
1017
23.61
1.11
22.93
1.24

    β-Phellandrene
1031
1029
24.36
2.30
—
—

    (Z)-β-ocimene
1043
1050
25.00
0.52
—
—

    γ-Terpinene
1061
1059
26.05
1.86
—
—

    Terpinolene
1088
1088
27.64
0.60
—
—

Total

35.14

16.19

Oxygenated monoterpenes

    1,8-Cineole
1035
1031
24.59
14.63
24.76
68.59

    β-Linalool
1100
1096
—
—
28.39
1.63

    trans-Sabinene hydrate
1100
1098
28.58
0.56
—
—

    trans-Pinocarveol
1139
1139
—
—
30.27
1.15

    Camphor
1145
1146
30.87
39.46
30.55
1.03

    Pinocarvone
1163
1164
—
—
31.50
0.85

    Borneol
1168
1169
31.75
2.79
—
—

    δ-Terpineol
1169
1166
—
—
31.81
1.83

    Terpinen-4-ol
1178
1177
32.30
1.18
32.27
0.57

    Cryptone
1186
1185
—
—
32.71
2.94

    α-Terpineol
1191
1188
32.99
1.56
33.04
1.11

    Myrtenol
1196
1195
—
—
33.26
1.01

    Verbenone
1209
1205
33.87
0.98
—
—

    Bornyl acetate
1287
1285
37.51
1.18
—
—

Total

62.34

80.71

Sesquiterpene hydrocarbons

    E-caryophyllene
1421
1419
43.41
1.07
—
—

Total

1.07

—

Sesquiterpene oxygenated

    Caryophyllene oxide
1581
1582
50.28
0.61
—
—

Total

0.61

—

Others

    3-Octanone
990
983
22.08
0.35
—
—

    Octen-3-ol
984
979
21.67
0.49
—
—

    No identified
—
—
—
—
31.27
0.47

    No identified
—
—
—
—
60.75
2.64

Total

0.84

3.11

RIa: retention indices; Rt:b: retention time; acalculated; breference.

Rašković et al. identified in R. officinalis essential oils 1,8-cineole (43.77%), camphor (12.53%), α-pinene (11.51%), β-pinene (8.16%), and camphene (4.55%) as the main constituents [38]. A lower concentration of 1,8-cineol (18.7–21.6%) was described by Ojeda-Sana et al. [34]. Touati et al. quantified the main components in the essential oil of L. dentata leaves, 1,8-cineole (33.54%) and camphor (18.9%) [39].

The above data agree with the assertion that the composition of the essential oil depends on several factors, such as climate and soil; hence, there may be differences in the percentage of its components.

3.3. Method Validation by GC-FID

The validation of the method for the quantification of the two main components in EO was performed to know the linearity, precision, and accuracy of the chromatographic method for the quantification of the peak components of EO-loaded NC. The chromatographic conditions and performance parameters were adjusted to provide a simple analysis with the best peak resolution, reducing run time and lowering the cost of validation and analysis of essential oils.

The regression equation, the correlation coefficient, LOD, and LOQ of each main component used as standards were established and are shown in Table 2. The standard curves of each main component were prepared by plotting the response area versus concentration. The acceptance criterion for linearity is given by the correlation coefficient, which must be greater than or equal to 0.99 [40]. A good linearity response was obtained with the method developed for the two main components of each essential oil used as standards. These values make it possible to ensure that the methods are capable of quantifying the main compounds in the established range.

Table 2: Analytical parameters of linearity, LOD, and LOQ of the main components of essential oils in standard solutions.

Essential oil
Main component
Regression equation
Correlation coefficient (R²)
LOD (μg mL⁻¹)
LOQ (μg mL⁻¹)

R. officinalis
1,8-Cineol
y = 1.213x + 0.045
0.999
0.48
1.45

Camphor
y = 1.082x + 0.111
0.999
0.62
1.89

L. dentata
β-Pinene
y = 1.437x − 0.057
0.999
0.15
0.45

1,8-Cineol
y = 1.226x + 0.186
0.999
1.28
3.88

The results obtained for the LOD and LOQ were between 0.48 and 1.89 μg·mL⁻¹ and 0.15 and 3.88 μg·mL⁻¹ for EO-R. officinalis and EO-L. dentata, respectively (Table 2). These results can be compared with those obtained by Fancello et al. for the components of Citrus limon var. pompia leaf essential oil. They calculated and obtained the limits of detection and quantification in a concentration range 50 to 150 µg·mL⁻¹ and 100 to 300 µg·mL⁻¹, respectively [41]. In comparison with these results, the values obtained in this work make it possible to guarantee that the method is capable of detecting trace amounts, either in free or encapsulated essential oils.

Accuracy was determined by analyzing five concentrations of the standard solution using a standard addition technique. A percentage recovery close to 100% indicates high accuracy of the analytical method. Miao et al. established an analytical method for quantitative analysis and chemical fingerprinting of volatile oils from Alpinia oxyphylla. They also established that the validated GC-FID method showed a good recovery with recovery percentages close to 100% [42]. In Table 3 are shown the values of percentage recovery and SD for accuracy, precision intermediate, and reproducibility of the main components of essential oils in NC.

Table 3: Analytical parameters of accuracy, precision intermediate, and reproducibility of the main components of essential oils in NC.

Essential oil
Main component
Accuracy (% recovery ± SD)
Repeatability (% recovery ± SD)
Precision intermediate (% recovery ± SD)

R. officinalis
1,8-Cineol
100.53 ± 4.46
97.26 ± 4.41
98.80 ± 4.87

Camphor
99.98 ± 5.03
99.19 ± 3.97
100.36 ± 4.84

L. dentata
β-Pinene
91.17 ± 5.57
98.02 ± 4.77
98.40 ± 4.97

1,8-Cineol
100.98 ± 4.90
101.95 ± 5.07
99.31 ± 5.75

Values are expressed as mean ± SD.

3.4. Method Applicability

3.4.1. Preparation and Characterization of EO-Loaded Polymeric Nanoparticles

The nanoprecipitation technique described by Fessi et al. [33] was used for EO-loaded NC preparation. Previously, it had been established that the ideal size in a biological system having loaded NC is about 200 nm [43]. The size of each EO-loaded NC was greater than 200 nm with homogeneous distribution and stability during 8 weeks at 25 ± 2°C (Figure 2). A percentage of encapsulation loading and efficiency are shown in Table 4. Specifically, a %EE of 59.03% for 1,8-cineol and camphor content in total R. officinalis essential oil and 41.15% for β-pinene and 1,8-cineol in total L. dentata essential oil were obtained, indicating that at least 59.03% and 41.15% of the R. officinalis and L. dentata essential oils, respectively, were encapsulated during the preparation of the NC. These results were lower than those reported with NC of Origanum vulgare ssp and Thymus capitatus essential oils (96 ± 4% and 91 ± 1%, respectively) [44]. The results revealed that the in situ properties of the NC-polymer determine the %EE of essential oil components. For example, Lugo-Estrada et al. used a headspace solid-phase microextraction GC-FID method to demonstrate that the %EE of Thymus vulgaris essential oil in NC of ε-polycaprolactone was close to 96% [45].

(a)

(b)

Figure 2: Size distribution of nanoparticles of EO-R. officinalis (a) and EO-L. dentata (b) measured by the DLS technique (8 weeks at 25 ± 2°C).

Table 4: Characterization of EO-loaded polymeric NC.

Essential oil
Size (nm)
PDI
Zeta potential (mV)
Main component
% EL
% EE

R. officinalis
226.50 ± 5.46
0.197 ± 0.032
54.47 ± 0.45
1,8-Cineol
2.95 ± 0.14
44.13 ± 3.13

Camphor
2.41 ± 0.13
14.90 ± 1.20

L. dentata
235.62 ± 7.50
0.214 ± 0.022
50.40 ± 0.75
β-Pinene
1.56 ± 0.30
28.69 ± 5.42

1,8-Cineol
3.18 ± 0.44
12.46 ± 1.71

Values are expressed as mean ± SD (n = 3).

The results of %EL obtained in the present work may be due to the volatile nature of the EO and were in agreement with the findings on the loading of oregano essential oil in chitosan nanoparticles, which have been reported by Hosseini et al. They developed formulations of chitosan nanoparticles with oregano essential oil with a percentage of encapsulation loading in a range of 1.32 to 2.12% [22]. The hydrophobic characteristics of EO make them a good candidate for encapsulation in nanoparticular systems via nanoprecipitation. However, it is important to emphasize that the encapsulation of a complex natural product, such as EO, is a process that represents greater difficulty compared to the encapsulation of a drug, and it requires studies of optimization of EO encapsulation [46].

Finally, a number a studies employing UV-VIS spectroscopy have been conducted to investigate encapsulation efficiency and loading capacity of EO-NC. A disadvantage of these UV techniques is that they generate an overlap of essential oil components signal, which may obtain an overestimation, and the UV-vis methods used have not been previously validated.

4. Conclusions

In this study, the chemical compositions of the essential oils from leaves of R. officinalis and L. dentata were determined. β-pinene, 1,8-cineole, and camphor were identified as the main components of the two essential oils. In this study, the validated analytical method allowed the simultaneous quantification of two main components of each nanoencapsulated essential oil, allowing the direct monitoring of these components in biological tests in vitro and in vivo. Hence, it is of great importance, due to the lack of analytical methods by direct GC-FID, to analyze these nanoencapsulated essential oils reported in the literature.

Data Availability

Currently, the data obtained have been used in the patent process in Mexico. The patent involves the formation of nanoparticles and the quantification of essential oils and their antioxidant activity for topical application. Therefore, the data cannot be released and they could not be sent until the moment of acceptance of the patent.

Conflicts of Interest

The authors declare that there are no conflicts of interest regarding the publication of this article.

Acknowledgments

The authors gratefully acknowledge the financial supports PN-CONACYT-México/2014-248560, CB-CONACYT-México/2016-284813, and PAICYT-UANL/IT658-18. Perla Giovanna Silva Flores is thankful to the CONACYT-México Program of Scholarships for Postgraduate Study 254012.

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