Termination of Repeat Testing in Chemical Laboratories Based on Practice Guidelines: Examining the Effect of Rule-Based Repeat Testing in a Transplantation Center

Background Although the automation of instruments has reduced the variability of results and errors of analysis, in some laboratories, repeating a test to confirm its accuracy is still performed for critical and noncritical results. However, the importance of repeat testing is not well established yet, and there are no clear criteria for repeating a test. Materials and Methods In this cross-sectional study, all repeated tests for 26 biochemical analytes (i.e., albumin, alkaline phosphatase (ALP), alanine aminotransferase (ALT), amylase, aspartate aminotransferase (AST), bilirubin total (BT), bilirubin direct (BD), blood urea nitrogen (BUN), calcium, chloride (Cl), cholesterol total (CholT), creatine kinase (CK), creatinine (Cr), glucose, gamma-glutamyl transferase (GGT), high-density lipoprotein-cholesterol (HDL-c), iron, lactate dehydrogenase (LDH), LDL-c, lipase, magnesium (Mg), phosphorus (Ph), protein total (ProtT), total iron binding capacity (TIBC), triglyceride (TG), and uric acid) were assessed in both critical and noncritical ranges over two consecutive months (routine subjective test repeats in the first month and rule-based repeats in the second month). To determine the usefulness of test repeats, differences between the initial and verified results were compared with the allowable bias, and repeat testing was considered necessary if it exceeded the allowable bias range. All causes of repeat testing, including linearity flags, delta checks, clinically significant values, and critical values, were also documented. All data, including the cause of repeats, initial and verified results, time, and costs in the two consecutive months, were transferred to Microsoft Excel for analysis. For comparison of data between the months, Student's t-test was used. Results A total of 7714 repeat tests were performed over two consecutive months. Although a significant decline (38%) was found in repeated tests in the second month (P < 0.001), there was no significant change in the percentage of unnecessary repeats (77% in the first month and 74% in the second month). In both consecutive months, AST and ALT were the most commonly repeated tests, and delta check was the most common cause of repeat testing. Mg, ALP, AST, and lipase showed the highest rates of necessary repeats, respectively (the least stable tests), while albumin, LDL, and CholT tests showed the highest rates of unnecessary repeats, respectively (the most stable tests). The total cost and delay in turnaround time (TAT) due to repeated testing decreased by 32% and 36%, respectively. Conclusion Although repeat testing has been shown to be unnecessary in most cases, having a strict policy for repeat testing appears to be more valuable than avoiding it completely. Each laboratory is advised to establish its own protocol for repeat testing based on its own practice.


Introduction
e delivery of proficient laboratory services is the cornerstone of modern healthcare systems, contributing to about 70% of all medical decisions [1]. Providing highquality and uninterrupted laboratory services requires many procedures to prevent problems. With the current technological advances, the accuracy of analyses has increased significantly, making today's equipment much more accurate than before. In contrast to preanalysis and postanalysis stages (as the main sources of laboratory error), the analysis phase accounts for about 0.1-3.8% of total laboratory errors [2][3][4][5].
So far, repeat testing has been a component of laboratory quality control activities for verification of data prior to releasing them out. Although in the analysis stage, automation of instruments has reduced the variability of results and errors, in some laboratories, repeating a test to ensure its accuracy is still performed for critical and also noncritical results [2,6]. e main causes of repeat testing include values above and below the analytical measurement range (AMR), delta checks, clinically significant values, and critical values. A large number of repeated data reported by clinical biochemistry laboratories agree with the initial findings and are classified as unnecessary repeats when evaluated based on the criteria.
Unfortunately, there are no definite criteria to repeat a test. e test repetitions, which are not based on any specific criteria, not only cause an increase in the turnaround time (TAT) or delayed reporting but also consume reagents that consequently increase the cost of laboratory tests [7]. erefore, the present study aimed to determine the current rate of test repeats, their causes and usefulness, TAT, and costs associated with the repetition of biochemical laboratory tests before and after designing a protocol as a novel practice.

Materials and Methods
is cross-sectional study was conducted in the Clinical Chemistry Laboratory of Abu-Ali Sina Hospital (Shiraz, Iran), which is a liver transplantation center, from May 2020 to July 2020. is study was designed in accordance with the Declaration of Helsinki and carried out after obtaining approval from the Ethics Committee of Shiraz University of Medical Sciences (IR.SUMS.MED.REC.1399.020). Generally, the annual test volume for the clinical chemistry laboratory of the hospital is approximately seven million. To avoid false or erroneous routine results, repeat testing is performed for some critical and noncritical data, according to automated flags of autoanalyzers and also the operator's opinion, such as flags of linearity (values above AMR), delta checks, clinically significant values, and critical values. Although the cutoff values for AMR (linearity) and critical values are well known and documented, there are no strict cutoff points for clinical significance and delta checks; therefore, the repeat rules in our laboratory seem to be relatively subjective.
All serum specimens from both hospitalized patients and outpatients, undergoing repeated tests over two consecutive months, were included in this study. Icteric samples were also included, as we could not avoid them in our center, which is a hepatic disease and transplantation center. e exclusion criteria were the specimens subjected to preanalysis errors, such as lipemia and hemolysis, and flags for values below AMR (mostly related to a low sample volume) that needed to be repeated with a new sample (the main sources of preanalysis error) [9,10].
A laboratory investigation of the samples was carried out using two identical Dirui-1200 autoanalyzers. e results of the tests were obtained by running the separated sera after centrifugation. Two levels of internal quality control (QC) materials, that is, level 1 (normal) and level 2 (high), were evaluated in this study. Both analyzers were calibrated periodically and maintained daily for quality control. Also, the performance of the two analyzers was compared weekly, using regression analysis to confirm similar results. e repeat test runs were performed on the same sample in the same run on the same analyzer (as the initial one). Moreover, for the second month, we designed some test repeat rules, and all employees were required to follow them for repeat testing. e defined causes of test repeats in the protocol included: (1) Linearity (high AMR): high cutoff values are extracted from the kit instructions. (2) Delta check: it measures the difference between a patient's sequential test results [11]. e cutoff values are extracted according to the following formula: where RCV is the reference change value (allowable delta value); CVA is the analytical variation (from quality control); and CVI is the intraindividual coefficient of variation [8].  Table 2 presents our test repeat rules (cutoff ranges for defined causes). It should be noted that for some results, there were some overlaps between different causes.

Statistical Analysis.
All data, including the dates, names of the tests, causes of test repeats, initial and verified results, time, and cost, for both consecutive months, were entered in Microsoft Excel for analysis. e absolute value and percentage of the difference between the two test runs were calculated for each value and then compared with the allowable bias limit. If the absolute difference between the final confirmed value and the initial result was greater than the College of American Pathologists/Clinical Laboratory Improvement Amendments (CAP/CLIA) allowable bias, the initial result was considered as an identified error, and the test was performed for the third time; the average of the two agreeing results was then reported. For comparison of data between the two consecutive months, Student's t-test was performed.

Results
A total of 142,480 biochemical tests and 7905 repeat tests were performed over two months, including routine (subjective) test repeats in the first month and rule-based repeats in the second month. A total of 191 test repeats were excluded from the analysis due to values below AMR, hemolysis, and lipemia (final sample volume: 7714). A significant decline (38%) in the repeated tests was found during the second month (P < 0.001) ( Table 3). e AST and ALT were the most commonly repeated tests in each month and also in both months. Figure 1 shows the share of each analyte in the total repeat tests, as well as the most common cause of test repeats for each analyte in both months. e initial and repeated (verified) results were evaluated for various analytes, and the percentage of change in the values (bias) was calculated.
ere was no significant difference (<1% bias) between the initial and repeated (verified) results in 4666 (60.5%) repeated tests. As shown in Figure 2, the bias pattern was relatively similar in both groups, and comparison with the allowable inaccuracy level (bias) determined the percentage of unnecessary repeats in each month ( Figure 3) [13].
Although in both groups, the most common causes of test repeats were delta values after protocol designation, there was a decline in the causes of clinically significant values, as shown in Figure 4. e Mg, ALP, AST, and lipase tests had the highest rates of necessary repeats, respectively (the least stable tests), while albumin, LDL, and CholT showed the highest rates of unnecessary repeats, respectively (the most stable tests). Besides, the total cost and delay in TAT due to repeat testing decreased by 32% and 36%, respectively.

Discussion
Although many strategies are used to eliminate laboratory errors, these errors still prevail [14,15]. e improved reproducibility by intelligent automation has led to a reduction in the variability of results and errors of analysis, with remarkable improvements in the quality of laboratory results [2]. e practice of repeating a test to prevent false results is necessary when testing is performed using instruments with poor analytical precision [16]. In many laboratories, this practice has persisted for critical value measurements, despite major advances in areas, such as automation, precision, and quality assurance [16][17][18]. A summary of data from the College of American Pathologists (CAP) Q-Probes survey suggested that 61% of laboratories still repeated testing for critical values [19]. Generally, noncritical laboratory test results are not repeated before being available [6]. Elimination of routine repeat testing can help reduce the test cost and TAT. Our hospital is a referral inpatient and outpatient liver transplantation center with highly complicated cases and highly abnormal biochemical results. erefore, we deal with a high number of repeated tests for confirmation in critical and noncritical ranges. To determine whether these duplicate tests can ensure or improve the accuracy of the results, we performed an audit of repeat testing to evaluate its usefulness, estimate the delay caused by repeat testing, and quantify the cost of repeating the tests.
In the present study, 7,714 repeat tests for 26 routine biochemical analytes were evaluated over two consecutive months to determine the accuracy of the results. In repeated test runs, 60.5% of the results showed no significant change after the test repeat (bias: 0-1%), and 75.5% of the repeats were within the acceptable limits of difference; therefore, they were considered unnecessary. Although there was a significant decline in the percentage of repeats, cost, and TAT in the second month with protocol designation, there was no significant change in the percentage of unnecessary repeats (77% in the first month vs. 74% in the second month). Also, delta check was the most common cause of test repeat in both months, although repeats due to clinical significance decreased after implementing the protocol, which could be due to the determination of objective criteria.
Overall, ALT and AST were the most common repeated analytes in both evaluated months, each accounting for more than 10% of total repeats. is could be explained by the subspecialty of our center in hepatic diseases and liver  transplantation, besides the high daily number of specimens with highly elevated liver enzymes that require at least one episode of repeat testing with dilution. e Mg, ALP, AST, and lipase tests had the highest number of necessary repeats, respectively (the least stable tests); the enzymatic nature of these analytes might make them so sensitive. On the other  [16,20]. In a similar study by Rodrigues et al., the usefulness of test repeat was evaluated for routine biochemical tests whether they were critical or noncritical. Overall, 81.3% of repeats were classified as unnecessary. Creatinine and urea were the most repeated tests, respectively, and among analytes that were common with our study, phosphorus, LDH, calcium, and ALP accounted for the highest percentages of necessary test repeats [21].
A comprehensive study by Deetz et al. evaluated the repeat laboratory results of 30 common laboratory tests (including 14 routine clinical chemistry analytes). In general, 3.8% of the total routine chemical analytes were repeated, which is consistent with our test repeat percentage after protocol designation. About 339 errors (necessary to repeat) were detected, most of which were related to values below AMR, followed by values above AMR and values within AMR (delta checks, critical values, and reviews). ese findings suggest that when the initial results are within the AMR, repeated testing is unnecessary [7]. In another study by Onyenekwu et al., only 17 (0.7%) out of 2308 test repeats were significantly different from the initial values; however, only four analytes (sodium, potassium, calcium, and Mg) in the critical range were evaluated in this study [6].
Rule-based repeat testing in our clinical chemistry laboratory improved the number of repeated tests, costs, and TAT, without compromising the accuracy. Although the results of our investigation agree with those of previous studies, the necessity of repeat testing seems to depend on the laboratory type, and further evaluations in different types of medical centers are needed. For example, our center is a liver transplantation center with a high number of daily requests for liver function tests and many icteric samples, which potentially act as a source of error and interference in chemical tests. erefore, dilutions must be used every day, even in the absence of high AMR (linearity) flags. Overall, each laboratory is suggested to follow its own policies and protocols for repeat testing and verification of the results. In this study, we assessed a high number of biochemical analytes in a highly variable and abnormal range. However, conducting experiments in a single specialized center, using only one type of automated analyzer, is still the main shortcoming of this study.

Conclusion
Although repeat testing has been shown to be unnecessary in most cases, having a strict policy for repeat testing appears to be more valuable than avoiding it completely. Each laboratory is advised to establish its own protocol for repeat testing based on its own practice.

Data Availability
e data used to support the findings of this study are included within the article.

Conflicts of Interest
e authors declare that they have no conflicts of interest. Acknowledgments e authors would like to thank Shiraz University of Medical Sciences (Shiraz, Iran) and also the Center for Development of Clinical Research of Nemazee Hospital for their contribution. e authors also extend our gratitude to Dr. Nasrin Shokrpour for her editorial assistance.  Journal of Analytical Methods in Chemistry