Trace Elements and Heavy Metal Contents in West Algerian Natural Honey

Analysis of trace elements and heavy metals in honey is essential for honey quality and safety and also monitoring environmental pollution. This study aimed to evaluate the composition of thirty-seven honey samples of different botanical origins (14 multifloral and 23 unifloral) obtained from beekeepers located in the west region of Algeria. Inductively coupled plasma-mass spectrometry (ICP-MS) and atomic absorption spectroscopy (AAS) methods were used to determine the levels of 19 elements in honey (K, Na, Ca, Mg, Mn, Cu, Fe, Zn, V, Cr, Co, As, Ru, Rh, Cd, W, Pt, Au, and Pb). Ru, Rh, Pt and, Au were not detected in any of the tested honey samples. The most abundant minerals were K, Ca, Na, and Mg ranging within 153.00–989.00 mg/kg, 33.10–502.00 mg/kg, 13.30–281.00 mg/kg, and 20.80–162.00 mg/kg, respectively. Fe, Mn, Zn, and Cu were the most abundant heavy metals while Pb, V, Cr, W, Co, and Cd were the lowest ones (<1 mg/kg) in the honey samples surveyed. Several honey types, lavender, rosemary, mild white mustard, thyme, milk thistle, carob tree, orange tree, Euphorbia, Eucalyptus, camphor, jujube tree, sage, and harmal, were studied, and the statistical analysis was carried out using principal component analysis (PCA) and hierarchical cluster analysis (HCA) techniques to evaluate the data. The results showed that the analyses of mineral content were sufficient to determine the floral origin and their variability may be related to geochemical and geographical differences. On other hand, all elements detected were at levels below safe thresholds.


Introduction
Bees are one of the most useful insects for humans: they pollinate our crops and produce honey [1,2]. Nowadays, they play a new role in indicating pollution and the quality of the environment, through produced honey [3,4]. Te bee forages for several kilometers in search of nectar; on its way, its body accumulates particles of metals and pollutants present in the environment which it transports to the hive [5].
Contaminants can be anthropogenic or natural and may pollute the soil with diferent fractions in many ways [6]. Pollutants have been shown to be absorbed by plants growing in contaminated soil and have higher levels in their tissue compared to other plants grown in control soils [7,8].
Tere is a close relationship between beehive products, notably honey, and plants, which means that honey inherits various characteristics and biological properties of plants with respect to their respective botanical sources and growing surface [9]. Due to this relationship, undesirable compounds or residues can be found in honey if plants or soil have been exposed to these substances [10]. Among the most hazardous contaminants for human health and the environment which can be found in honey, we can cite heavy metals [11,12]. Tey are found naturally in the Earth's crust, but their presence in the environment has increased due to high anthropogenic activity [13]. For several decades, it has been shown that exposure to metals implies serious damage [14]. Teir bioaccumulation and amplifcation in living systems lead to undeniable efects and diseases [15,16]. Qualitative and quantitative analyses of heavy metals in honey, in addition to the classical approach, would allow a reliable and reproducible system to establish the botanical origin [17] and geographic traceability [18]. Te environmental aspect of a region has also been assessed using minerals and heavy metals present in honey [19]. Several studies have been conducted to establish the relationship between pollution in a region and the presence of contaminants in honey [20][21][22]. Te main elements found in diferent kinds of honey throughout the world, considered as a profling parameter, can be divided into two groups according to the literature: major elements or gold macroelements (Na, K, Ca, Mg, P, S, and Cl) and trace elements including heavy metals (Al, Cu, Pb, Zn, Mn, Cd, Tl, Co, Ni, Rb, Ba, Be, Bi, U, V, Fe, Pt, Pd, Te, Hf, Mo, Sn, Sb, La, I, Sm, Tb, Dy, Sd, T, Pr, Nd, Tm, Yb, Lu, Gd, Ho, Er, Ce, Cr, As, B, Br, Cd, Hg, Se, and Sr) [20,[22][23][24][25][26][27][28]. Te latter can afect the nervous system, kidney, liver, and respiratory functions. Some metals, such as cadmium, arsenic, nickel, and chromium, are carcinogenic [29]. Exposure to heavy metals is implicated in more severe pathologies like multiple sclerosis and neurodegenerative diseases (Alzheimer's and Parkinson's disease) [29][30][31]; they could even play a role in triggering psychological and neurological disorders such as autism [32]. It is also important to mention that the presence of certain minerals in honey is essential for the evaluation of its nutritional and curative quality [33]. Besides minerals and trace elements, honey is composed of various carbohydrates, polysaccharides, oligosaccharides, favonoids, vitamins, minerals, waxes, aroma compounds, pollen grains, pigments, and enzymes which also contribute to its profling and authentication [34]. In Algeria, honey is considered as a precious product because of its high nutritional value as well as its therapeutic virtues widely popular traditionally [35,36]. Te relatively mild climate in Algeria and abundant varied fora allow the production of a variety of honey [37]. According to the Algerian Center for Quality Control and Packaging (CACQE), national honey production was estimated in 2011 at 33,000 quintals and in the same year 150,000 tonnes of honey, mainly from China, India, and Saudi Arabia, were imported (CACQE, https://www.cacqe. org/). Te growing interest in honey, which is considered as a noble substance, requires vigilance to its authenticity and originality to preserve national production, avoiding every sort of fraud. Based on multiple searches done on the PubMed database using diferent keywords, it is noteworthy that the number of studies on the physicochemical properties of Algerian honey, particularly in the west region, is very scarce. Te present study aims to identify major and trace elements contained in diferent samples of honey collected in west Algeria. Besides physicochemical methods, we used principal component analysis (PCA) and hierarchical clusters analysis (HCA) techniques to diferentiate Algerian honey, from diferent botanical origins, according to their mineral content and the choice of elements with a higher discriminating power.

Honey Samples.
A set of thirty-seven Apis mellifera intermissa honey samples (500 g each), namely, S1-S37, was collected in 2017 and 2018 from eight geographical provinces (Tlemcen, Ain Temouchent, Sidi Bel Abbes, Mostaganem, Mascara, Tiaret, Naâma, and Bechar) in west Algeria (Figure 1). Table 1 shows the botanical and geographical origins of the honey samples studied. Samples were taken directly from beekeepers with a guaranteed origin, stored in airtight plastic containers, and then kept in a refrigerator at 4°C until ICP-MS and AAS analysis processes.

Reagents and Solution.
High-purity deionized water acquired by passing distilled water through a water purifcation system (demineralizer HLP 20, Hydrolab, Poland) was used to prepare the solutions. Certifed single-element standard solutions (1000 mg/L) used to prepare the calibration curve were of the highest purity grade (99.999%) and were supplied by Ultra Scientifc (North Kingstown, RI, USA). Other reagents were of analytical grade unless otherwise stated. Honey samples were digested with Suprapur ® grade nitric acid (HNO 3 65% m/m, Merck, Germany). A recovery test was performed using a single-element solution and two reference materials, namely, Standard Reference Materials (SRMs) Tomato Leaves and Pine Needles (Pinus taeda) (SRM 1573a and SRM 1575a, resp., National Institute of Standards and Technology (NIST), Gaithersburg, USA).

Instrumentation.
Elemental analysis was carried out by using 820-MS inductively coupled plasma quadrupole mass spectrometer (ICP-MS; Varian, Mulgrave, Australia) equipped with an SPS3 autosampler (Varian, Australia) and a MicroMist nebulizer type (Varian). A MARS Express microwave mineralizer system (CEM, Matthews NC, USA) of Tefon reaction vessels was used in the digestion procedures. Te reaction vessels were cleaned using 10 mL of concentrated nitric acid before each digestion. Te multielemental determination was also determined by the atomic absorption spectroscopy (AAS) method using a spectrometer SpectrAA 280 FS with autosampler SPS3 (Varian, Australia), which was equipped with a deuterium lamp, hollow cathode lamp for each element, and an air-acetylene burner.
Appropriate results in terms of accuracy and sensitivity, low cost, and quickness make AAS a suitable procedure for determining the concentrations of alkaline and earth alkaline elements in the honey samples investigated. Tables 2 and  3 show the instrumental parameters for ICP-MS.   2 Journal of Analytical Methods in Chemistry 2.4. Analytical Determination. Trace element and heavy metal determination was performed by the inductively coupled plasma-mass spectrometer (ICP-MS) and atomic absorption spectroscopy (AAS) using the operating conditions and emission wavelength lines listed in Tables 2 and 3. Glass and plastic material was cleaned and kept in 10% (v/v) nitric acid solution for at least two days. Te material was then rinsed three times with high-purity deionized water before being used. Approximately 0.5 g of each honey sample was digested with 10 mL of 65% HNO 3 (v/v) in Tefon vessels. Te sealed vessels were put into the microwave mineralizer MARS Express (CEM, USA). A blank digest was carried out in the same way. Te microwave mineralization was performed stepwise at 400 W and 363 K, at 800 W and 393 K, and at 1600 W and 483 K. Te cooled digestion solution was then diluted to 50 mL using highpurity deionized water. Tis solution was fnally used for the determination of V, Cr, Co, As, Ru, Rh, Cd, W, Pt, Au, and Pb of the honey samples, performed with an inductively coupled plasma-mass spectrometer equipped with a concentric nebulizer, a quartz torch with quartz injector tube, and cyclonic spray chamber ( Table 2). Te concentration of K, Na, Ca, Mg, Mn, Cu, Fe, and Zn ions was determined by the AAS method using a spectrometer SpectrAA 280 FS with autosampler SPS3 (Table 3). To avoid sample ionization during potassium analysis, Schinkel bufer solution (mixture contents: 10 g/L cesium chloride and 100 g/L lanthanum chloride) was used. Each sample was measured in triplicate by AAS and ICP-MS detection.

Results of Minerals in Honey Samples.
Te concentrations of trace element and heavy metals found in the honey samples collected from thirty-seven locations situated in west Algeria are given in Tables 5 and 6. Validation parameters of the analytical procedure such as: limit of detection (LOD), limit of quantifcation (LOQ), precision, accuracy, and uncertainty budget are listed in Table 4. A detailed validation of the analytical procedure was performed including the performance parameters limit of detection (LOD) and limit of quantifcation (LOQ); precision, accuracy, and an uncertainty budget (Table 4). Precision and percentage recovery of the analytical procedure were determined by using randomly selected honey samples individually spiked with known spiked concentrations of the trace elements and heavy metals studied (K, Na, Ca, Mg, Mn, Cu, Fe, Zn, V, Cr, Co, As, Ru, Rh, Cd, W, Pt, Au, and Pb) and used as positive controls. Te mean percentage recoveries of the determined trace elements and heavy metals ranged between 74.51% (Co) and 117.32% (Fe) ( Table 4), which point out good accuracy, precision, and validity of the method employed. Te results of mineral content in honey were found relatively low and varied over a range within 0.04-0.16% of the total composition, which is in agreement with the composition of nectar honey [22,38]. A total of 19 trace elements and heavy metals were determined. It should be noted that the concentrations of 19 elements were variable depending on the foral origin of honey [19]. Among them, the most abundant elements were K, Ca, Na, and Mg with average levels (mean ± SD) of 532.46 ± 212.69 mg/kg, 113.92 ± 95.38 mg/ kg, 83.40 ± 64.85 mg/kg, and 100.83 ± 33.88 mg/kg, respectively. Te west Algerian honey samples in the present investigation showed a wide range of K contents (153.00-989.00 mg/kg) accounting for 80.08% of the total minerals present in honey from Bouzedjar (S21). Following our data, similar amounts of K in honey samples were previously reported from the Azilal and Beni Mellal provinces in Moroccan (256-1023 mg/kg) [39] and Tunisian (172.48-976.75 mg/kg) honey [40]. Overall, the concentrations of K in west Algerian honey were higher than those reported from Turkey (1.18-268 mg/kg) [20], the West Bank in Palestine (42.80-585.00 mg/kg) [41,42], and Jableh and Tartous provinces in the western part of Syria (38.2-174 mg/ kg) [43], but the levels were lower than those reported for Portugal (117.55-2590.60 mg/kg) [44,45], Italy (237-6520 mg/kg) [23,25], Libya (1120.1-1980.6 mg/kg) [46], and Spain (1615-3770 mg/kg) [47][48][49][50][51][52]. Eucalyptus honey showed the second highest concentration (987.00 mg/ kg, S27) of K among the honey samples investigated while carob tree honey contained the lowest amount (153.00 mg/ kg, S12). High concentrations of K were also present in the mild white mustard (946.00 mg/kg, S6), lavender (808.00 mg/kg, S1), and milk thistle (802.00 mg/kg, S23) honey when compared to the other honey samples studied. Te concentration of K in the present study is also much higher than the three Citrus spp. (Citrus) honey samples from Syria (38.2-174 mg/kg) [43]. Tis discrepancy may be due to the geographical variation in the sources of honey [52]. A high mean value (113.92 ± 95.38 mg/kg) of Ca was observed among the investigated west Algerian honey samples with the concentrations ranging within (33.10-502.00 mg/kg) which were higher than those of honey samples reported for Morocco (19. [46] of all the Mediterranean regions considered. It would be interesting, due to the presence of the high amount of this mineral, to propose it in a strategy for the prevention of osteoporosis. Sage honey showed the highest concentration (502.00 mg/kg, S34) of Ca, followed by multiforal honey (377.00 mg/kg, S14), Eucalyptus honey (287.00 mg/kg, S25), and milk thistle honey (215.00 mg/kg, S23). Interestingly, there are some similarities between the Ca contents of some types of west Algerian honey and those coming from   [43]. In the case of the  [56]. Te concentrations of Cu in the west Algerian honey ranged from 1.66 to 9.62 mg/kg (mean ± SD 4.90 ± 1.64 mg/kg). Te highest concentrations of Cu were in multiforal, jujube tree, and carob tree honey (9.62 mg/kg, S4; 8.97 mg/kg, S33; and 7.86 mg/kg, S10, resp.), but Cu was not detected in more than a quarter of the investigated honey. Unfortunately, the levels in approximately 54% of the investigated honey samples are above the PTWI (3.5 mg/kg b.w.) for Cu established by Joint FAO/WHO [56]. Generally, Cu is transferred and accumulated in food under the infuence of the environment and also due to human contributions (fertilizers and pesticides) [57]. Copper levels in west Algerian honey are slightly higher than those from Morocco (≤0.1 mg/kg) [39], Tunisia (0.12-0.34 mg/kg) [40], Portugal (0.00-5.35 mg/kg) [45], France (0.06-1.71 mg/kg) [53], and other Mediterranean countries (Table 7). In summary, Eucalyptus honey contained the highest K and Na values. Sage honey is rich in Ca while milk thistle honey is rich in Mg. Lavender honey contained high Fe levels while the carob tree is the richest in Zn. Our study shows also that the mild white mustard honey contained highest level of Mn, whereas jujube tree and carob tree honey comprised Cu in high concentrations. Generally, the west Algerian honey samples are rich in minerals. Te latter contains a signifcant proportion of microelements and therefore are valuable food products. Tese latter are a signifcant fraction of  Morocco [55] Tunisia [39] Portugal [43] Portugal [44] Spain [46] Spain [47] Spain [48] Spain [49] Spain [50] N �   France [53] France [54] Italy [24] Italy [22] Turkey [19] Greece [25] Cyprus [25] Egypt [25] Palestine [40] Palestine [41] Syria [42] Libya [45] N � micronutrients making honey valuable food products. Some of these elements are vital, and others are merely desirable or benefcial. Indeed, they have signifcant roles in the activation of certain enzymes; these include Mg, Fe, Mn, Zn, and Cu, which play an important role in the metabolic transformations in the human body, while K, Ca, and Na are essential in building strong bones and teeth, muscle contractions, nerve signals, regulating heartbeat, and fuid balance within cells [58]. Teir defciencies play critical roles in many disorders such as hypertension and osteoporosis [59]. Some honey samples studied are important sources of micronutrients useful to the proper functioning of the human body, and therefore their consumption is highly recommended, especially in case of defciency. To verify the quality of the west Algerian honey, in addition to Mn, Fe, Zn, and Cu, it is very important to assess and monitor the concentrations of other heavy metals and metalloids, which are potentially toxic. Tey include arsenic, lead, chromium, cadmium, vanadium, tungsten, cobalt, ruthenium, rhodium, platinum, and gold. In the present study, the relative concentrations of these elements in the honey samples decreased in the following order: Pb > V > Cr > W > As > Co > Cd > Ru, Rh, Pt, and Au. All of them were detected at levels <1 mg/kg. Te resulting data, summarized in Table 5, were consistent with the ranges indicated for honey from other studies [20,26,50,53,60]. Lead is considered a strict contaminant and is toxic to living organisms, even at very low concentrations. Its content in honey is examined in several studies.
Te range values of Pb content in honey samples from the west Algerian region were 0.54-132.73 μg/kg. Pb was found at high concentrations in the multiforal honey samples (132.73 μg/kg, S17; 91.36 μg/kg, S30; and 89.44 μg/kg, S21) and was absent in milk thistle (S8). Furthermore, the observed concentrations of Pb were higher than those measured in Morocco (≤0.1 mg/kg) [38], Tunisia (0.01-0.05 mg/ kg) [40], Greece (<0.08 mg/kg), Cyprus (<0.08 mg/kg), Egypt (<0.08 mg/kg) [26], Spain (46.32-31.50 μg/kg) [50], and Syria (<0.082 mg/kg) [43]. However, the concentrations of Pb in the examined honeys were lower than in the following honeys: Palestinian (0.51-0.94 mg/kg) [42], French (3-101 mg/kg) [54], and Italian (28-304 mg/kg) [23], and (9-209 mg/kg) [25]. However, concentrations found were lower than those found in Palestinian (0.51-0.94 mg/kg) [42], French (3-101 mg/kg) [54], and Italian (28-304 mg/kg, [23]; 9-209 mg/kg, [25] honey. In this study, the mean level of Pb in honey samples is 24.73 μg/kg, which is still within the PTWI of Pb for adults (25 μ/kg b.w.) [56]. Te extremely high Pb levels found in Italian honey depicted contamination caused by external sources or by incorrect procedures during honey processing as reported by Pisani et al. [23]. Vanadium is a natural component of the Earth's crust, which is widespread in nature. One of the main sources of environmental pollution by V comes from the combustion of fossil fuels [61]. Te mean value of V content in honey samples from the thirty-seven locations in west Algeria was 13.52 μg/kg. Among the honey samples surveyed, camphor and thyme honey samples are the richest in V (106.51 μg/kg, S26; 79.73 μg/kg, S11, resp.), whereas rosemary honey (0.81 μg/kg, S29) was the poorest one. In this study, the levels of V were lower than those reported by Conti et al. [25] ( Table 7). Tere is currently no JECFA assigned reference health standard for vanadium. Chromium is abundant in the environment in a trivalent or hexavalent state. It is found in the trivalent state, at low concentrations, in a wide range of foods [62]. Its content in foods can be greatly afected by anthropic and geochemical factors. Chromium has low toxicity in foods in part due to its low bioavailability. Currently, there is no formal recommended dietary allowance for Cr [63]. Tungsten is found naturally on Earth almost exclusively in the combined state with other elements in ores as wolframite and scheelite. Because it is a rare metal and its compounds are generally inert, the efects of W on the environment are limited [64]. Few honey samples from the west Algerian region contained quantifable concentrations of W. Te highest W concentration was found in harmal honey (24.30 μg/kg, S35). Besides, W concentrations of approximately 90% of honey samples were below LOQ. W was not investigated in others studies around the Mediterranean region. Terefore, it is not possible to comment on any diferences in the level of W in honey. Arsenic is a ubiquitous element with metalloid properties. It occurs naturally in organic and inorganic forms. Tis is the most toxic element and is considered carcinogenic to humans [65]. Te major use of As compounds is in agriculture and forestry as pesticides and herbicides [66]. Te levels of As ranged from 1.61 to 21.56 μg/kg with a mean concentration of 5.86 μg/kg (Table 5). Te highest levels of As were found in the multiforal and milk thistle honey (21.56 μg/kg, S4 and 18.20 μg/kg, S8, resp.) while its concentration was lower than LOQ in lavender honey (S1). Also, low concentrations were found in orange tree and thyme honey (1.61 μg/kg, S16 and 1.75 μg/kg, S7, resp.). Our values for As were similar to the Libyan values (0.006-0.018 mg/kg) [46] and were below those from Morocco (0.00-0.045 mg/ kg) [67], Greece, Cyprus, and Egypt (<0.08 mg/kg) [26]. Very high As contents have been reported in some honey samples from France (nd-8.0 mg/kg) [54] and Italy (<25 mg/ kg; 2.8-11.1 mg/kg, resp.) [23,25], which may be from  [53], Spanish (0.015-0.720 mg/kg) [60], and Italian (1.0-17 mg/kg and 2.9-30.2 mg/kg, resp.) [23,25] honey. Besides, the levels of Co were similar to those published for honey coming from Greece, Cyprus, and Egypt (<0.03 mg/kg) reported by Karabagias et al. [26]. Highest concentrations of Co were found in harmal honey (21.19 μg/ kg, S35), whereas the lowest concentrations were in multiforal and orange tree honey (0.36 μg/kg, S17; 1.02 μg/kg, S16, resp.). Overall, our data indicate that there are low levels of Co contamination in the west Algerian honey samples. Tere is currently no upper level of intake established by WHO and JECFA for cobalt. Cadmium is a metallic element that occurs naturally at low concentrations in the environment. It has been added to the environment through anthropogenic activities such as Cd metal production in industrial processes or by the use of phosphate fertilizers in agricultural soils [68]. Te level of Cd in the west Algerian honey samples ranged from 0.24 to 8.14 μg/kg, which was lower than that reported for the honey from most of the Mediterranean countries. Te levels of Cd of half of them are, however, higher than those from Turkey (<1 μg/kg) as reported by Altun et al. [20] (Table 8). Moreover, Cd was present at a high concentration in thyme honey (8.14 μg/kg, S7) and below the limit of quantifcation in multiforal honey (S17). In 2005, the Joint FAO/WHO established a PTWI for Cd of 7 µg/kg b.w. [66]. It is worth noting that the average Cd content (1.23 μg/kg) for the examined honeys was lower than the PTWI value. [66]. Ruthenium, rhodium, and platinum are part of the platinum-group metals belonging to the group VIII transition metals. Tey are generally grouped with gold and silver as precious metal commodities. Each of the metals occurs naturally in its native form, and in economically exploitable deposits, the elements occur overwhelmingly as mineral species. Te uses of these metals are distributed among the chemical, electrical, jewellery, and glass industries. In order to fnd out if these metals have found their way into the west Algerian honey, their levels were monitored in all the samples considered. In this study, the results show that Ru, Rh, Pt, and Au were lower than the limit of quantifcation in any of the tested honey samples. In summary, the levels of heavy metals and metalloids were, generally, low and comparable with the levels in honey from uncontaminated regions [20,39,40], indicating that the west Algerian honey is of good quality.

Multivariate Statistical Methods.
Te principal component analysis (PCA) and hierarchical cluster analysis (HCA) of the 37 honey samples were performed by XLSTAT 2014.5.03 software for the trace elements and heavy metals shown in Tables 5 and 6 except Ru, Rh, Pt, and Au, which were not detected excluded. Te variance estimate results (eigenvalues) obtained are presented in Table 9. PCA is a very powerful pattern recognition technique that describes the variance of a large dataset of intercorrelated variables with a lesser set of independent variables [27]. Analyzing the data in Table 9, it can be observed that PCA account contains together for 60.89% of the total variance of the dataset. S25 S14 S6 S27 S15 S21 S1 S8 S10 S23 S34 S12 S7 S16 S20 S11 S13 S3 S2 S35 S4 S30 S31 S18 S33 S5 S36 S24 S32 S19 S29 S26 S9 S37 S17 S28 0 level of similarity in which explanations are joined together may be used to create a dendrogram. In the present work, the optimal linkage distance level was 410, represented by the dashed line in Figure 2. It represents a relative measure of similarity among analyzed honey samples. From Figure 2, it can be seen that three groups were formed, which can be identifed as follows: eleven samples were clustered in group I (S1, S6, S8, S10, S14, S15, S21, S22, S23, S25, and S27); one sample in group II (S34); and twenty-fve samples in group III (S2, S3, S4, S5, S7, S9, S11, S12, S13, S16, S17, S18, S19, S20, S24, S26, S28, S29, S30, S31, S32, S33, S35, S36, and S37). It can be observed that group I contains lavender, mild white mustard, milk thistle, carob tree, Euphorbia, and Eucalyptus honey types with high mineral contents, which showed similarities to each other. Group II contains sage honey type. Furthermore, group III comprises rosemary, thyme, orange tree, camphor, harmal, jujube tree, and many multiforal honey types with low levels of minerals, which correlated to each other. Tese results show that west Algerian honey samples vary according to their geographic origins and also to their mono or multiforal characteristics (Table 1). In addition, it is to underline that clear separation among the samples was obtained from several diferent geographic locations in western Algeria. From the PCA, it can be verifed that the characteristics that infuenced honey sample clustering the most were major elements K, Na, Ca, Mg, and Fe, whereas minor ones such as Cr, Co, V, W, As, Cd, and Pb were considered less important. In summary, the visualization of the data by HCA and PCA ofers enough information to develop a classifcation method to determine the botanical origin of honey samples considering the mineral element composition.

Conclusion
Te present chemical study provided a detailed picture of the trace element and heavy metal contents of natural honey from west Algeria, through the analysis of 19 elements in 37 types of uniforal honey (i.e., lavender, rosemary, mild white mustard, thyme, milk thistle, carob tree, orange tree, Euphorbia, Eucalyptus, camphor, jujube tree, sage, and harmal) and multiforal honey. Trace elements and heavy metals were determined and measured by ICP-MS and AAS methods. In summary, K was the most abundant element. K, Ca, Mg, and Na accounted for 97.92% of the total minerals present in honey investigated; this content places them among very dark and amber honey, which corresponds to their intrinsic golden/dark amber color. Eucalyptus honey contained the highest levels of K and Na. Sage honey is rich in Ca while milk thistle honey is rich in Mg. Additionally, lavender honey contained high Fe values while the carob tree is the richest in Zn. Mild white mustard honey contained highest level of Mn, whereas jujube tree and carob tree honey comprised Cu in high concentrations. Potentially toxic heavy metals and metalloids such as Pb, V, Cr, W, As, Co, and Cd were detected at concentrations <1 mg/kg. Furthermore, Ru, Rh, Pt, and Au were absent in all honey samples. Te use of multivariate statistical methods such as HCA and PCA shows that mineral contents may represent a major discriminating strength based on the botanical origin of honey samples. Overall, our results indicated that the west Algerian honey is rich in essential minerals benefcial for human health. Te levels of the trace elements and heavy metals were below the PTWI established by JECFA. Finally, it can be concluded that the west Algerian honey is uncontaminated and therefore represents a good indicator for monitoring environmental pollution with metals. Moreover, the levels of mineral contents are at safe levels for human consumption.

Data Availability
Te data used to support the fndings of this study are included within the article.