Growth and Characterization of Organic Marine Dye Compound : 6-Amino-8 a-methoxy-5-methyl-4 , 7-dioxo-1 , 1 a , 2 , 4 , 7 , 8 , 8 a , 8 b-octahydroazireno [ 2 ' , 3 ' : 3 , 4 ] pyrrolo [ 1 , 2-a ] indol-8-yl ] methyl Carbamate

Single crystals of 6-amino-8a-methoxy-5-methyl-4,7-dioxo-1,1a, 2,4,7,8,8a,8b-octahydroazireno[2',3':3,4]pyrrolo[1,2-a]indol-8-yl]methyl carbamate (Mitomycin), an organic marine dye material has been grown from solution by slow evaporation at ambient temperature. The growth of crystals has been carried out at various pH values and the growth was confirmed at pH 6. The chemical composition of the grown crystals was determined by the FTIR spectra. The crystalline nature and its various planes of reflections were observed by the powder XRD. The grain size is found to be 500 microns using SEM studies and the NLO activity of the grown crystal has been checked by second harmonic generation (SHG) test.


Introduction
In day to day life water is an essential for both human being and aquatic animals.Sustainability of any human community hinges on adequate supply of potable water.Marine dyes usually have synthetic origin and complex aromatic molecular structure which make them more stable [1][2] .Nonlinear optical (NLO) materials have gained considerable attention due to their practical applications in the field of optoelectronics 1,2 .The development of NLO materials led to compounds potentially suitable for application in frequency conversion, optical telecommunication, image processing, optical computing and data storage devices [3][4][5][6] .Quinazolinone family-type crystals have over the years been subjected to extensive investigation by the researchers for their non-linear optical properties 7 , 8 .Among the disulfonic acid, quinoline is the simplest molecule with second harmonic generation efficiency of about onethird of that of the well known KDP 9,10 , it is expected to get improved NLO properties.This paper defines the crystal structure of mitomycin of molecular formula C 15 H 18 N 4 O 5 and 6-amino-8a-methoxy-5-methyl-4,7-dioxo-1,1a,2,4,7,8,8a,8b-octahydroazireno-[2',3':3,4]pyrrolo[1,2-a]indol-8-yl]methyl carbamate.This has been investigated by the FTIR studies, its crystalline nature was studied by the powder XRD, the transmittance and absorbance of electromagnetic radiation was studied through UV-Visible spectrum and its grain size is 500 microns was confirmed by SEM studies.Hence the aim of this paper is to report the growth, spectroscopic studies and NLO activity of crystals for the first time.

Results and Discussion
The pH values 6 of the solution clearly indicate the confirmation of crystal growth.The crystal growth structure has been confirmed by UV, IR, XRD and SEM analysis.

FTIR
In FTIR spectra were recorded on Shimadzu IFS 66V FTIR, amide functional group appeared at 3440 cm -1 with sharp absorption and also giving C=O stretching absorption at 1638 cm -1 .It was further confirmed by observing C=O overtone absorption appeared at 3810 cm -1 Wavelength, nm %T %T cm -1 and 3919 cm -1 .The strong absorption at 1030 cm -1 was assigned to C-O group due to its stretching vibrations.The strong absorption at 1564 cm -1 confirms the presence of COO functional groups along with the amide group.The asymmetric stretching absorption of -CH 3 group was appeared at 2364 cm -1 and its symmetric stretching absorption was appeared at 2224 cm -1 .Also, strong absorption at 1411 cm -1 was due to bending absorption of alkyl group.The absorptions were summarized in Table 1 and spectra were given in Figure 3.

Crystal surface analysis by SEM
Surface analysis of 6-amino-8a-methoxy-5-methyl-4,7-dioxo-1,1a, 2,4,7,8,8a,8b-octahydroazireno[2',3':3,4]pyrrolo[1,2-a]indol-8-yl]methyl carbamate is carried out through scanning electron microscopy.The magnification 100 times has been studied for this crystal.The maximum magnification studied in the equipment is 3, 00, 000 times with a resolution of 3 nm.The surface of the crystal was coated with gold to make the sample conducting.From the Figure 5, it is clear that the size of the crystals is 500 microns thick.Further the surface is very smooth without any defects.

Table 1 .
Growth and Characterization of Organic Marine Dye Compound 1800 Frequencies of the fundamental vibrations of GPC