Separation of Functionalized 5 , 6-Disubstituted-1 , 10-Phenanthroline for Dye-Sensitized Solar Cell Applications

5,6-Epoxy-1,10-phenanthroline is used as a convenient starting material for 5-hydroxy-6-Aryl-1,10-phenanthroline ligands containing carboxylic and sulfonic groups useful for further anchoring of the sensitizer on TiO2 for dye-sensitized solar cells (DSCs). Based on the crystal growth of the mixture of products, a convenient separation route for the extension of the p-system on 5,6-disubstituted-1,10-phenanthroline was used to develop a novel series of functionalized 1,10-phenanthroline ligands with electron-withdrawing end-capping group. Also, we report the epoxy opening of 5,6-epoxy-1,10-phenanthroline by aromatic amines stoichiometrically in re�uxing water and ethanol in the absence of any catalyst. e dyes were characterized by H-NMR, FT-IR, UV-Vis, and X-ray single crystal diffraction analyses. It crystallizes in the monoclinic space group C 2/c, a = 20.920(4) Å, b = 10.340(2) Å, c = 16.187(3) Å, ββ = 92.30(3),V = 3498.6(12) Å, and Z = 8.e reaction details and features were described in detail.


Introduction
e dye-sensitized nanocrystalline solar cell (DSC) offers special opportunities in the realm of renewable energy sources primarily stemming from its use of low-cost materials and its lack of stringent conditions required for device manufacturing [1].In a dye-sensitized cell, the immobilization of the sensitizer on the nanocrystalline TiO 2 surface allows efficient electronic communication between the lightharvesting center and the semiconductor substrate.e immobilization of the sensitizer is achieved by attaching anchoring groups (e.g., carboxylate [2,3], sulfonate [4,5], salicylate [6], phosphonate [7][8][9], carbonyl [10,11], catechol [12], and acetyl acetonate groups [13]) to the surfaces of the mesoporous TiO 2 nanoparticles [14].To further increase the performances of dye-sensitized solar cell, it is of paramount importance to engineer ligands at a molecular level and thus to endow sensitizers with both panchromatic absorption and a relatively high molar extinction coefficient.erefore, to accelerate the discovery and improvement of better performing sensitizers, easily accessible strategies for the functionalization of 2,2-bipyridyl (bpy) or 1,10-phenanthroline (phen) derivatives are required [15].A distinct property of this class of chelating agents, phen and bpy, in particular, is their  electron de�ciency that makes them excellent  acceptors capable of stabilizing metal ions in lower oxidation states.Furthermore, due to the presence of low-energy  * orbitals of the ligand, metal complexes can be characterized by strong metal-to-ligand charge-transfer (MLCT) absorption bands in the visible spectrum and red-shi�ed �uorescent emissions [13][14][15][16][17] which is indicated as suitable ligand for dye-sensitized solar cell application.Due to the combination in phen of these distinct structural and chemical properties, the increasing interest in the chemistry of phen derivatives has prompted the development of efficient synthetic procedures for the chemical �ctionalization of the phen nucleus at the various ring positions [18].e 5-and 6positions of phen are the most susceptible to electrophilic substitutions.Reaction with commercial bleach under phasetransfer conditions can afford, in good yield, 5,6-epoxy-1,10phenanthroline which represents a good intermediate for functionalization of the 5-position of phen with different binding sites for analytes [18].In this paper, we report such an efficient synthetic route for the preparation of a series of symmetrically 5,6-disubstituted-1,10-phenanthroline being endcapped by electron-withdrawing groups.is end-capping diversity will allow us to tune the photophysical properties of the corresponding Ru-based sensitizers in a rational manner.

Experimental
All chemicals and solvents were purchased from Merck & Aldrich and used without further puri�cation.IR spectra were recorded on a Perkin-Elmer 597 spectrometer and 1 H-NMR spectra were recorded by using a Bruker 250 MHz spectrometer.
e X-ray diffraction measurements were made on a STOE IPDS-II diffractometer with graphite monochromated Mo-K radiation.Cell constants and orientation matrices for data collection were obtained by least-squares re�nement of diffraction data from 4711 unique re�ections.Data were collected to a maximum 2 value of 58.5 ∘ in a series of  scans in 1 ∘ oscillations and integrated using the Stoe X-AREA [19] soware package.e data were corrected for Lorentz and Polarizing effects.e structures were solved by direct methods [20] and subsequent difference Fourier maps and then re�ned on  2 by a full-matrix least-squares procedure using anisotropic displacement parameters [21].All hydrogen attached to carbon atoms were added in idealized positions and hydrogen atoms of the water molecule, NH and NH 2 group were found in difference Fourier map.All re�nements were performed using the X-STEP32 crystallographic soware package [22].A summary of crystal data, experimental details, and re�nement results is given in Table 1.
For the synthesis of 5,6-epoxy-1,10-phenanthroline, 1 mmol (198 mg) of phenanthroline, dissolved in 20 mL of chloroform, is added to 250 mL of 0.6 M aqueous sodium hypochlorite (commercial bleach), the pH of which has been adjusted to 8.5 with concentrated HCl.Tetrabutylammonium hydrogen sulfate (0.2-1 equiv) is added and the mixture is stirred at room temperature until TLC indicates that all of the phenanthroline has reacted.e layers are separated and the organic layer is washed with a large excess of cold water, dried, and evaporated at room temperature.e crude epoxide was puri�ed by recrystallization from a mixture of chloroform and hexanes (5 : 1) to give a product [23].For synthesis of L 1, the phenanthroline 5,6-epoxide (100 mg, 0.5 mmol) and 4-amino-benzenesulfanic acid (A) (88.53 mg, 0.5 mmol) were taken in a mixture of water and methanol (50 ml) and re�uxed for 14 h.Aer evaporation at room temperature, orange and yellow crystals were appeared.For synthesis of L 2 1,10-phenanthroline-5,6-epoxide (L) (100 mg, 0.5 mmol) was dissolved in 30 ml of methanol which contained 109 mg (0.5 mmol) of 2-phenylamino-benozic acid

Results and Discussion
Reaction of 5,6-epoxy-1,10-phenanthroline with a variety of nucleophiles results in hydroxy-dihydro intermediates that upon dehydration can afford not only 5-substituted phen derivatives useful for further manipulations but also interesting ditopic ligands featuring the phen nucleus derivatized at the 5-position with chelating groups [18].Based on experimental results, the opening of the strained threemembered epoxy ring usually occurs in nonaqueous media in presence of a Lewis acid catalyst like Al 2 O 3 , Li + , Mg 2+ [24].Additionally oen elevated temperature and excess of nucleophiles are required.ere are a few reports where the use of a catalyst has not been necessary [25,26].For example, aminolysis of epoxide by an a-amino acid ester proceeds smoothly in re�uxing tri�uoroethanol [25].e same reactions fail in water.Azizi and Saidi found that the aliphatic and aromatic amines cleave an epoxide ring in water at room temperature without any Lewis acid catalyst [26].Herein, aminolysis of 5,6-epoxy-1,10-phenanthroline is potential in generating two products.We obtained phen as a minor product also in the reaction of epoxide with aniline group in the standard conditions for preparation of L ′ 1 and L ′ 2 (Figure 1).A plausible mechanism for the deoxygenation may include formation and subsequent denitrogenation of L ′ 1 and L ′ 2 .e easy denitrogenation at room temperature that we found was apparently stimulated by the aromatization of the central ring as in the case of reactions.Apparently, aromatization of the central ring is a driving force for this elimination [27].
As shown in Figures 2 and 3, the main absorption peaks in the IR spectra of compounds are determined.Generally, in the IR spectrum of the phen, strong bands were observed in frequency region between 1400 and 1650 cm −1 , the one band occurring at 1505 cm −1 , the second appearing at 1590 cm −1 , and the third band at 1423 cm −1 .In the present study, IR spectra of L 1 and L 2 show (C-N) band related to phen, at 1422 and 1420 cm −1 , respectively, indicating the partial double bond character.e ring frequencies associated with phen are observed at 1497, 1601 cm −1 and 1509, 1594 cm −1 , for L 1 and L 2 .e (C-H) of phen bands appear at 2876, 2946, 2965 cm −1 and 2885, 2941, 2970 cm −1 , respectively.e (A) compound actually has the zwitterions structure that contains the -NH 3 + and -SO 3 − groups.In the IR spectrum of L 1 , there are a number of bands between 2735 cm −1 and 2735 cm −1 due to combination bands of the NH 3 group.e CH stretching vibration of anilinic ring is observed at 2883, 2905, and 2921 cm −1 [28].e  (ph) vibrations are observed at 1601, 1578, 1547, 1422, and 1319 cm −1 .Bands at 1173, 1037, 684, and 561 cm −1 derive from presence of SO 3 .e vibration band at 1037 is characteristics of the vibrations of the sulfur atoms linked to the aromatic cycle and of S=O symmetric [29].e asymmetric S=O vibration is observed at 1173 cm −1 .It is important to note, the disappearance of  (NH 2 ) stretching frequency of amine group and appearance of  (NH) stretching at 3445 cm −1 in the IR spectrum of L 1 reveals the formation of L 1 (Figure 2).Two signi�cant vibrations at 1659 and 1262 occur in IR spectrum of L 2 .e band at 1659 cm −1 is attributed to the C=O stretching vibration of the carboxyl group and that 1259 cm −1 can be ascribed to the C-N stretching vibration.e N-H stretching vibration at 3339 cm −1 was removed.ese results suggest that (B) was linked to L (Figure 3).e UV-Vis absorption spectra of the compound in ethanol solution are given in Figures 4 and 5. Phen is characterized in aqueous solution by UV absorption at 229 and 265 nm, the latter attributed to - * transition to the lowest-energy excited singlet state, - * .e absorption band at 230 nm of L 1 and 229 nm of L ′ 1 are the characteristic of the phen.e absorption band of L 1 at 265 nm shows blue shi to 255, may be due to being overlapped with that of (A) at 249 nm.In the UV-Vis spectra of L 2 and L ′ 2 , two absorption bands were observed at 230, 257 nm and 229, 262 nm, respectively.ese bands are characterized to the - * transition of the phen rings.But the band at 265 nm, due to the lowestenergy singlet state, shis to 257 nm in spectrum of L 2 .ese absorption bands are good evidence for presence of L in the product.In 1 H NMR spectra of L 1 , the protons of phen give signals at 8.06, 8.87, and 9.21 ppm which are slightly deshielded.e signal observed at 7.4 ppm is assigned to the H f present in L 1 .Other proton of anilinc ring, H e , is slightly shielded and is observed in the up�eld region at 6.76 ppm.ese observed changes show the formation of L 1 as shown in Figure 5.  e molecular structure of L ′ 1 and the atom-numbering scheme are shown in Figure 7.
ere are C-H⋯  interactions and also different weak hydrogen bonds (Table 2), stabilizing the three-dimensional crystal structure.Signi�cant - interactions are found in the crystal structure.
To gain further insight into the electron density distribution within the frontier and other close lying orbitals, we performed quantum chemical calculations on the L 1 using density functional theory (DFT) as shown in Figure 8. e simulations have been performed with the Gaussian 09 program.e level of LAN L2DZ was carried out.In the dye-sensitized solar cells, the LUMO of the dye must be sufficiently high in energy to promote efficient charge injection into the semiconductor �lm and the HOMO should be sufficiently low in energy for efficient regeneration of the oxidized dye by the hole transport material.Figure 8 shows schematic representation of frontier orbitals of L 1 , along with isodensity plots of HOMO, HOMO −1 and LUMO, LUMO +1 orbitals.Here, the chemical structures of the phen and amino benzene solfonic acid have an important effect on the electronic properties of the L 1 .e L 1 presents a HOMO-LUMO energy gap of 4.48 e� signi�cantly larger than the gap calculated for many dye-sensitized solar cell.In the ground state, the electron density of L 1 for the highest occupied molecular orbital (HOMO) and HOMO −1 is shared by the phenyl moiety of phen and amino benzene solfonic group.Whereas the LUMO resides on the phen for L 1 and shows no overlap with the amino benzene solphonic group.e predicted effect is an increase of the HOMO-LUMO gap of L 1 , which is in good agreement with the experimental observations.As shown in the �gures, these energy levels are able to inject energetic electrons into the conduction band of TiO 2 .
Further work on the photovoltaic performance of 5,6-functionalized 1,10-phenanthroline is in progress, and we believe improvements can be achieved by a thorough optimization of ruthenium complexes with respect to, for example, other functionalized 5,6-functionalized 1,10phenanthroline.

Conclusion
In summary, two new symmetrically 5,6-functionalized 1,10phenanthroline bidentate ligands have been synthesized from 5,6-epoxy-1,10-phenanthroline and carboxyl and sulfonyl aryl ligands.is easy and convenient synthetic strategy allowed us to introduce either electron-withdrawing anchoring groups on the p-conjugated-phenanthroline core.ose ligands have potential for ruthenium sensitizer for dyesensitized solar cells. 1 H NMR analysis revealed that all the new synthesized ligands were exclusively obtained.Synthesized dye can be relieved by disappeared amine group in FT-IR spectra.is simple synthetic approach is expected to expedite the development of antenna-type ligands for ruthenium sensitizers.

Disclosure
Crystallographic data for the title compound has been deposited to the Cambridge Crystallographic Data Centre (CCDC no.888674).Copies of available materials can be obtained free of charge on application to the Director, CCDC, 12 Union Road, Cambridge CB2 IEZ, UK.

F 1 :
Molecular structure of the investigated ligands and the procedure of reactions.

F 6 :
1 H-NMR spectra of aromatic region of L 1 and L 2 dissolved in DMSO solvent at room temperature.

F 7 :
ORTEP of L ′ 1 with the numbering scheme.ermal ellipsoids are at the 50% probability level.For L 2 , 1 H NMR signals around 7.77, 8.48, and 9.08 ppm arise from the phen protons (Figure 6).e signals of H d are deshielded due to the SO 3 group and observed at 7.86 ppm.e proton signals of the H g , H i and H f , H j appear at 7.20 and 7.31 ppm, respectively.e signals at 6.75 and 7.05 ppm are associated to H e and H k .All of these signals indicate that the production of L 2 is occurred.