Kynurenic Acid Content in Selected Culinary Herbs and Spices

Previous studies demonstrated that kynurenic acid (KYNA) is present in various types of food in varying concentrations.Therefore, the aim of the study was to check whether KYNA is present in culinary herbs and spices. Achieved results indicate that KYNA is present in all 19 selected culinary herbs and spices. The highest concentration of KYNA was found in basil and thyme, 14.08 and 8.87 μg/g, respectively, while the lowest content of KYNA was found in cumin and black pepper, 0.64 and 0.10 μg/g, respectively. This is the first report on the concentration of KYNA in culinary herbs and spices. The need for more detailed investigation of dietary supplementation with culinary herbs and spices containing KYNA is suggested.


Introduction
Kynurenic acid (KYNA) is a natural substance whose presence was first demonstrated in urine by Liebig in 1853 [1].It did not attract scientists' attention until the 1980s and 1990s when it was found that KYNA is an antagonist of ionotropic glutamate receptors, including N-methyl-D-aspartate (NMDA), -amino-3-hydroxy-5-methyl-4isoxazolepropionic acid (AMPA), and kainate receptors [2][3][4][5][6].Afterwards, it was also demonstrated that KYNA is an antagonist of alpha7 nicotinic receptors [7].Interestingly, both ionotropic glutamate receptors and alpha7 nicotinic receptors are mainly present in the brain.It was also proven that KYNA is present in the brain [8,9] and that it can be synthesized in the brain along the kynurenine pathway [10].An increased content of KYNA was found in schizophrenia, Alzheimer's disease [11,12], meningitis, autoimmune diseases, and inflammation [13].On the other hand, a decreased concentration of KYNA was found in Parkinson's disease [14], Huntington's disease [15], and multiple sclerosis [16].Due to varying results it is not possible to firmly state the role of KYNA in the central nervous system.Nevertheless, it should be emphasized that the penetration of KYNA through the blood-brain barrier in physiological conditions is limited [17].Therefore, a separate analysis of KYNA's presence and actions outside of the brain is necessary.It was found that KYNA is present in human blood and peripheral organs of the body [18][19][20].Additionally, it was indicated that KYNA is an agonist of GPR35 receptors which are mainly present in the gastrointestinal tract [21].Importantly, concentrations of KYNA in the digestive system gradually increase along the gastrointestinal tract.The lowest concentration was found in saliva while the highest in mucus of rat's ileum [22].The reason for this gradual increase in KYNA content is not known as is the source of KYNA in the gastrointestinal tract.It was proven that KYNA can be synthesized enzymatically in the human body from tryptophan administered orally [23].Furthermore, it was suggested that KYNA might be absorbed from the digestive system to the blood stream and then transported to other tissues.Intragastric administration of KYNA increased KYNA content in serum, liver, and kidneys in rats [24].Moreover, drinking water with added KYNA was accepted by animals and did not cause any toxic effects [24].Achieved results indicate that KYNA might be either synthesized in the human body or absorbed from food and drinks.
The role of KYNA in the periphery is not fully known.It was, however, shown that KYNA possesses numerous positive properties, including mainly antiulcerative, antioxidative, and anti-inflammatory properties.It may, therefore, positively influence a number of gastrointestinal tract pathologies, especially ulcers and colitis (see for review [25]).There may be some dispute whether KYNA plays a positive or a negative role in bowel diseases since its concentration in irritable bowel syndrome patients is decreased [26,27] while in inflammatory bowel disease patients is increased [28].Nevertheless, it seems as if KYNA possessed mostly positive properties in the gastrointestinal tract which suggests its daily consumption should be analysed and controlled.
Earlier studies showed that KYNA is a constituent of various types of food and that its concentration in food varies.The highest concentration of KYNA was found in vegetables and honey while the lowest was found in meat [29].Furthermore, it was shown that the content of KYNA is different in various parts of a plant-the highest content was found in leaves while the lowest was found in rootsand it was shown that a plant might either synthesize KYNA from its precursor, kynurenine, or absorb it from the ground [29].KYNA was also found in various herbs and herbal preparations; the highest concentrations of KYNA were found in St. John's wort, nettle leaf, birch leaf, elderberry flower, and peppermint leaf, all of which are believed to possess healing and protective properties when it comes to the digestive system.Therefore, the goal of this study was to investigate whether selected culinary herbs and spices that are of a plant origin contain KYNA.

Standard and
Reagents.Kynurenic acid (KYNA) was purchased from Sigma (St. Louis, MO, USA).All high performance liquid chromatography (HPLC) reagents were purchased from J.T. Baker (Deventer, Netherlands) or Sigma (St. Louis, MO, USA) and were of the highest available purity.For extraction of KYNA, cation exchange resin Dowex 50 WX4-400 purchased from Sigma (St. Louis, MO, USA) was used.

Methods.
Experiments were performed according to the method described in detail previously [29].In brief, samples of culinary herbs and spices were weighed and distilled water was added to them (1 : 10 w/v).They were then homogenized and centrifuged (5,000 rpm, 10 min) and 1 mL of supernatant was collected.Samples were then acidified with 50% trichloroacetic acid and vortexed.Denatured proteins were removed by centrifugation (12,000 rpm, 10 min).Samples acidified with 1 N HCl were applied to the columns containing cation exchange resin Dowex 50 prewashed with 0.1 N HCl.Subsequently, the columns were washed with 1 mL of 0.1 N HCl and 1 mL of water.The fraction containing KYNA was eluted with 4 mL of water.The eluate was subjected to HPLC (Dionex HPLC system; ESA catecholamine HR-80, 3 m, C18 reverse-phase column) and KYNA was quantified fluorometrically (Dionex RF2000 fluorescence detector; excitation 350 nm, emission 404 nm).The mobile phase consisted of 50 mM sodium acetate and 250 mM zinc acetate (pH 6.2), containing 5% of acetonitrile.The flow rate was 1.0 mL/min.Original KYNA was added to some of the samples as an internal standard.

Statistical Analysis. Samples were analysed in triplicate.
Data were presented as a mean value and a standard deviation (SD).

Results
HPLC profile of a substance isolated from culinary herbs and spices was compared with authentic KYNA.In all cases, the shape and the retention time of the peak of isolated substance and authentic KYNA were identical (Figure 1).

Discussion
KYNA was found in all analysed samples of culinary herbs and spices.Interestingly, the content of KYNA varied significantly between analysed herbs and spices; the content of KYNA in basil was about 140 times higher than the content of KYNA found in black pepper.Based on the fact that plants were found to contain, produce, and absorb KYNA from the ground [29] and that all analysed herbs and spices were of a plant origin, achieved results showing that all herbs and spices contain KYNA are not unexpected.Such conclusion is strengthened by the fact that KYNA content in leaves was found to be higher than its content in flowers and roots [29] while the majority of analysed herbs and spices originated from plants' leaves.Interestingly, though, the content of KYNA in herbs and spices was higher than the content of KYNA in all previously analysed foods, including broccoli, potatoes, and honey, which were thought to contain significant amounts of KYNA [30].Still, when considering a daily intake of KYNA, the amount provided through consumption of herbs and spices will most probably be lower in comparison to the amount provided by the most popular vegetables that contain significant amounts of KYNA, such as broccoli or potatoes.Nevertheless, various herbs and spices may be considered a valuable source of KYNA in an everyday diet since they are used with both vegetables and meats.The fact that they are commonly used along with meats might be meaningful bearing in mind the fact that meats are not a rich source of KYNA [30].Furthermore, it was shown that diets that contain high amount of meat, especially red meat, are unhealthy and cause numerous pathologies.One of the key findings indicates that high red meat consumption is so strongly correlated with diabetes that it should be treated as one of its risk factors [31].Furthermore, there are numerous studies showing that an increased consumption of red meat may lead to metabolic diseases, cardiovascular diseases, and obesity [32,33].Researchers also suggest that red meat consumption is directly correlated to some cancers occurring in the gastrointestinal tract.Meta-analyses indicate that the consumption of more than 50 g of red meat per day may lead to an increased risk of colorectal cancer; still, the crucial risk factor is the regularity of red meat consumption.Moreover, it was shown that red meat consumption may lead to a 27% increase in the risk of developing pancreatic cancer [34].These results were confirmed by a research, which showed that the consumption of processed meat and red meat increased the risk of developing pancreatic cancer by 68% and 50%, respectively.All in all, it may be concluded that diets rich in red meat lead to severe pathologies of not only gastrointestinal tract but the whole organism.Therefore, it seems vital to look for substances, which may protect or limit negative effects exerted by such diets.Despite the fact that the role of KYNA in the periphery is not yet fully known, numerous positive properties of KYNA were described in the literature.It was indicated that KYNA protects against duodenal and gastric ulceration caused by poisonous Atlantic shellfish [35] and reduces stress-and ethanol-induced ulceration in rats [36].Furthermore, it was shown that KYNA lowers hypermotility in the intestine and activity of xanthine oxidase in colon obstruction in dogs [37].KYNA was also found to possess anti-inflammatory properties [38].Moreover, it was shown that KYNA has antiproliferative properties in colon cancer in vitro [39].KYNA content was found to be lower in irritable bowel syndrome patients [26,27] while it was found to be higher in inflammatory bowel disease patients [28].Finally, an increased concentration of KYNA was found in mucus obtained from coecum or ascending colon in patients who suffered from colorectal cancer adenomas [40].
All in all, it seems that KYNA possesses mostly positive properties when it comes to the gastrointestinal tract.Its role in patients suffering from various bowel syndromes needs to be further investigated before any conclusions are derived.When it comes to an increased KYNA content in mucus from coecum or ascending colon in colorectal cancer adenomas patients, there is still no satisfactory explanation.On the one hand, KYNA was found to have antiproliferative properties.On the other hand, it was shown that cancer cells in the intestine produce KYNA more effectively when compared to normal intestine cells.Therefore, further investigation is necessary.As for now, an increased level of KYNA in such patients may be used as a novel marker in gastroenterology [40].
Bearing in mind all the facts pointed out above, culinary herbs and spices, which contain KYNA as was proven in the paper, may become an important dietary supplement.Culinary herbs and spices might be selected based on their content of KYNA, leading to an increase or a decrease in the amount of KYNA in a diet depending on the specific needs of a person.Such dietary supplementation using specific culinary herbs and spices needs further investigation.

Figure 1 :
Figure 1: Overlay of samples from integration view.HPLC identification and quantification of KYNA in culinary herbs and spices: (a) authentic KYNA 1 pmol; (b) HPLC profile of a substance extracted from basil; and (c) HPLC profile of a substance extracted from thyme.Note identical retention time and shape of the peak.

Table 1 :
KYNA content in analysed spices and herbs.