Journal of Chemistry
Volume 2016 (2016), Article ID 4546290, 10 pages
http://dx.doi.org/10.1155/2016/4546290

Research Article

Pollution Level, Sources, and Lung Cancer Risk of PM₁₀-Bound Polycyclic Aromatic Hydrocarbons (PAHs) in Summer in Nanjing, China

Shiqi Sun,1,2 Zhonghuan Xia,1,2,3,4 Tao Wang,1,2 Minmin Wu,1,2 Qianqian Zhang,1,2 Jing Yin,1,2 Yanchi Zhou,1,2 Hao Yang,2,3,4 Wenqi Wang,1,2 Yueming Yu,1,2 Jing Xu,1,2 and Cheng Chen1,2

¹Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, School of Environment, Nanjing Normal University, Nanjing 210023, China
²Key Laboratory of Virtual Geographic Environment, Nanjing Normal University, Ministry of Education, Nanjing 210023, China
³Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing 210023, China
⁴State Key Laboratory Cultivation Base of Geographical Environment Evolution (Jiangsu Province), Nanjing 210023, China

Received 18 June 2016; Accepted 22 September 2016

Academic Editor: Athanasios Katsoyiannis

Copyright © 2016 Shiqi Sun et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

This study concentrated on the pollution level, sources, and lung cancer risk of PM₁₀-bound polycyclic aromatic hydrocarbons (PAHs) in summer in Nanjing, China. PM₁₀ samples were collected in summer of the year 2015 in Nanjing. 16 USEPA (United States Environmental Protection Agency) priority PAHs were extracted and analyzed after sampling. The mean concentrations of PAHs and were and  ng/m³, respectively, being in a middle level among results from regions worldwide. According to the results of diagnostic ratios, PAHs originated mainly from traffic exhaust, especially diesel vehicle emissions. Owing to the inhalation exposure, the median values of incremental lung cancer risk (ILCR) were estimated to be , , , , , , , and for boys, male adolescents, male adults, male seniors, girls, female adolescents, female adults, and female seniors, respectively, indicating low potential lung cancer risk.

1. Introduction

Polycyclic aromatic hydrocarbons (PAHs) are a collective name of the organic compounds condensed by two or more benzene molecules or cyclopentadiene molecules. The USEPA fixed 16 parent PAHs as priority pollutants and these 16 compounds (16 USEPA priority PAHs) were widely concerning due to their carcinogenic properties and wide distribution in atmosphere. Besides their distribution in gas phase, PAHs are easy to adhere to atmospheric particulate matter like PM2.5 and PM₁₀, be inhaled, and stay on alveoli [1–3]. Researches have shown that PAHs in environment are mainly produced by human beings [4]. Vehicles, industrial production, and daily activities are important sources of PAHs. Based on BaP equivalent () in air, daily inhalation exposure (E) level and incremental lung cancer risk (ILCR) for human beings caused by particulate PAHs could be estimated. However, studies on ILCR caused by PAHs for population groups of different age and gender are limited.

Nanjing, lying on the southeast of China, is the capital city of Jiangsu Province. As a fast-developing industrial city, its population and economy are increasing rapidly, which has caused atmospheric pollution. Currently, most researches about particulate PAHs in Chinese cities focused on the main urban areas and the new urban areas are usually ignored, whereas the number of new urban districts has been increasing in recent years in China due to the rapid urban construction, with Nanjing being a typical example.

The objectives of this study were to investigate the pollution level of PM₁₀-bounded PAHs in summer in a new urban area of Nanjing, the main sources of PAHs in PM₁₀, and the ILCR caused by particulate PAHs for local population groups of different age and gender.

2. Materials and Methods

2.1. Sampling

The sampling site was located on the roof of a building in Nanjing Normal University, which is situated in one of the new urban districts of Nanjing, Jiangsu Province, China (Figure 1). The longitude and latitude position of the building is 32°07′N, 118°54′E and the altitude is 20 m. The sampling period is from July 30th to September 13th in 2015, with two PM₁₀ samples collected in each week. For each sampling, 24 h was included from 8:00 to 8:00 in the next day. Total of eleven PM₁₀ samples were collected. During the sampling period, the mean temperature of air was 25.8 to 34.3°C. The weather was mostly sunny and cloudy, which means no raining conditions. In summer, the wind direction is based on south and southwest and the wind scale is from 3 to 5.

Figure 1: Sample location in Nanjing, China.

Daily PM₁₀ samples were collected by an active air sampler (PM₁₀-300, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, China; operating at a constant flow rate of 0.3 m³/min), with glass fiber filters (GFFs) to collect particulate phase PAHs. Before sampling, the GFF was heated in a muffle furnace at 450°C for 4 hours. GFFs were then equilibrated in a desiccator (25°C, 40% RH) for 24 h and weighted before and after each sampling.

2.2. Sample Extraction and PAH Analysis

The GFF was extracted with 25 mL of a mixture of -hexane and acetone (1 : 1, v/v) by microwave extraction system (MES) (MARS2Xpress, CEM, USA). The tubes were heated to 100°C by microwave at the rate of 10°C/min and held for 10 min. After the extraction, the extract was concentrated in water bath of 37°C/min by using a rotatory evaporator (R-201, Shanghai, China). After concentration, extracts of particle phase PAHs were transformed to the alumina silica gel column for purification. The alumina silica gel column was eluted with 20 mL of -hexane followed by 50 mL 1 : 1 mixture of -hexane and dichloromethane at a rate of 2 mL/min. The eluted mixture from the column during cleanup was first concentrated to near dryness in the vacuum rotary evaporator (R-201, Shanghai, China) using a 37°C water bath. The residue was then transferred and diluted with -hexane and brought to exactly 1.0 mL by nitrogen blowdown (Eyela MG-1000) at room temperature (25°C). The samples were sealed in vials and stored at −4°C before being analyzed.

Quantitative analysis of the air sample extracts was done by gas chromatography with mass spectrometer detector (QP2010, Shimadzu, Japan). A 30 m × 0.25 mm i.d. × 0.25 μm film thickness HP-5MS capillary column was used. GC temperature was programmed from an initial 50°C (maintain 1 min) at a rate of 15°C/min up to 180°C and then at a rate of 5°C/min up to 250°C with a final holding time of 20 min. Helium was used as the carrier gas. 1.0 μL aliquot of the extract was injected, while the injector port was held at 280°C and operated in splitless mode at a flow rate of 1.0 mL/min. The head column pressure was 30 kPa. The mass spectrometer was operated in scan mode with an electron impact ionization of 70 eV, an electron multiplier voltage of 1200 V, and an ion source of 230°C. Concentrations were determined for 16 individual PAHs in all samples. They were naphthalene (NAP), acenaphthene (ACE), acenaphthylene (ACY), fluorene (FLO), phenanthrene (PHE), anthracene (ANT), fluoranthene (FLA), pyrene (PYR), benz(a)anthracene (BaA), chrysene (CHR), benzo(b)fluoranthene (BbF), benzo(k)fluoranthene (BkF), benzo(a)pyrene (BaP), dibenz(a,h)anthracene (DahA), indeno(1,2,3-cd)pyrene (IcdP), and benzo(g,h,i)perylene (BghiP).

2.3. Quality Control and Data Analysis

All solvents used were chromatography purity (Dikma, China). Alumina and silica gel (80–200 mesh; Dikma, China) were heated at 650°C in a muffle furnace (DLII-9, Beijing, China) for 10 h and kept in a sealed desiccator. Prior to use, the alumina and silica gel were reactivated at 130°C for 4 h immediately prior to use. All glassware was cleaned using an ultrasonic cleaner (KQ-500B, Kunshan, China) and heated to 400°C for 6 h.

Quantification was performed by the use of external calibrations which were obtained with PAH solutions at five concentration levels. The field and laboratory blanks were analyzed, and the concentrations of target PAHs in the field blanks were higher than those in the laboratory blanks. Concentrations in both blanks were more than one order of magnitude lower than those of real samples. All the results of samples were field blank corrected. Recovery of individual PAHs ranged from 66% to 103% with a mean value of 84%. Data analyzed in the article were not corrected for recoveries. The detection limits were in the range of 4.81–44.1 pg/m³.

2.4. Source Identification

Diagnostic ratios were used to identify the primary sources of PAHs in diverse phases. Some authors have pointed out that PAH compounds differing in relative molecular mass can be used as tracers to distinguish main sources [5–7]. In this study, diagnostic ratios of ANT/(ANT + PHE), BaA/(BaA + CHR), FLA/(FLA + PYR), IcdP/(IcdP + BghiP), BaP/BghiP, and FLO/(FLO + PYR) were calculated to determine the sources of particulate PAHs. Typical diagnostic ratios and corresponding judge criteria taken from previous literature were listed in Table 1.

Table 1: Typical diagnostic ratios and corresponding judge criteria.

Ratio
Specific value
Source identification
References

ANT/(ANT + PHE)
<0.1
Petroleum
[5]

>0.1
Combustion

BaA/(BaA + CHR)
<0.2
Petroleum

0.2–0.35
Mixed sources
[5]

>0.35
Combustion

FLA/(FLA + PYR)
<0.4
Petroleum

0.4–0.5
Petroleum combustion
[5]

>0.5
Grass, wood, & coal combustion

IcdP/(IcdP + BghiP)
<0.2
Petroleum

0.2–0.5
Petroleum combustion
[5]

>0.5
Grass, wood, & coal combustion

BaP/BghiP
<0.6
Nontraffic exhaust
[6]

>0.6
Traffic exhaust
[7]

FLO/(FLO + PYR)
<0.5
Gasoline vehicle emissions
[8]

>0.5
Diesel vehicle emissions

2.5. Risk Assessment

BaP equivalent concentration is often used in the estimation of inhalation exposure to PAHs and it was calculated according to

where BEC is concentrations of PAHs; is concentration of PAH congener ; TEFs are the toxicity equivalency factor (TEF) of PAH congener [9].

The citizens in Nanjing were divided into eight population groups according to the age and gender: children (4–10 years), adolescents (11–17 years), adults (18–60 years), and seniors (61–70 years) of both males and females. Daily inhalation exposure (E) level for each population group was calculated based on

where is daily inhalation exposure level (ng/d), is concentrations in the sampling site, and IR is inhalation rate (m³/d) [10–12]. We treated and , which followed lognormal and normal distribution, respectively, in (2) probabilistically.

The incremental lifetime cancer risk (ILCR) of population groups in Nanjing caused by PAHs inhalation exposure was calculated using

where is the incremental lifetime cancer risk of the inhalation exposure (dimensionless); is the cancer slope factor for BaP inhalation exposure (a lognormal distribution with a geometric mean of 3.14 (mg kg⁻¹ day⁻¹)⁻¹ and a geometric standard deviation of 1.80) [13]; is the daily inhalation exposure level (ng/day); is the exposure frequency (day/year) (111 days for summer); is exposure duration (year) (for children: ; for adolescents: ; for adults: ; for seniors: ) [14]; is conversion factor (10⁻⁶ mg/ng); is body weight (kg) [15, 16]; is average lifespan for carcinogens (25550 day). We treated , , and , which obeyed lognormal, lognormal, and normal distribution, respectively, in (3) probabilistically.

3. Results and Discussion

3.1. Pollution Level

Comparisons of PAHs concentrations in PM₁₀ among Nanjing and other regions are shown in Table 2. The mean concentrations of PAHs and were 7.49 ± 2.60 ng/m³ and 1.21 ± 0.24 ng/m³, respectively. The mean concentration was lower than 2.5 ng/m³, the daily limited standard of atmospheric BaP in the newest ambient air quality standard of China (GB3095-2012), whereas it was greater than 1 ng/m³, the European Union standard, indicating atmospheric PAHs pollution [17].

Table 2: Comparisons of PAHs concentrations (ng/m³) in PM₁₀ among Nanjing and other regions.

Region
Sampling time
PM fractions
Concentrations of PAHs (ng/m³)

Amount of PAHs
References

Nanjing, China
Summer, 2015
PM ₁₀
7.49
1.21
16
This study

Beijing, China
Summer, 2006
PM₁₀
60.05
—
18
[18]

Shijiazhuang, China
Summer, 2013
PM₁₀
26.9
—
14
[19]

Chengdu, China
Summer, 2012
PM₁₀
64.21
9.54
16
[10]

Taiyuan, China
Summer and autumn, 2009-2010
PM₁₀
63.8
13.79
16
[20]

Guangzhou, China
Summer, 2002
PM₁₀
26.01
—
16
[21]

Shanghai, China
Years 2011-2012
PM₁₀
36.9
5.4
16
[22]

Malaysia
Years 2013-2014
PM2.5
2.79
0.57
16
[23]

Eastern Germany
Summer, 2006
PM₁₀
3.21
—
12
[24]

Veneto, Italy
Summer, 2011
PM₁₀
0.2
—
8
[25]

Kathmandu Valley, Nepal
Years 2013-2014
TSP
155
26.2
15
[26]

Ulsan, Korea
Summer, 2011
PM₁₀
21.9 ± 6.6
5.53
16
[27]

Kumasi, Ghana
Summer, 2011
PM₁₀
3.0 ± 3.5
0.30
16
[28]

25 ± 7.9
5.30

PS: pristine site; CC: city center.

Compared with data from other regions (Table 2), the concentration of PAHs in summer in Nanjing was lower than that in Beijing, Shijiazhuang, and Guangzhou in summer. Also, it was lower than that in Taiyuan in both summer and autumn and Shanghai in the whole year. For cities from all over the world, the concentration of PAHs in Nanjing was lower than that in Ulsan, the city center of Kumasi in summer, and Kathmandu Valley in the whole year. However, it was still higher than those in some areas, including Eastern Germany, Veneto, and the pristine site of Kumasi in summer and Malaysia in the whole year. concentration of PAHs in PM₁₀ in this study was lower than that in Chengdu in summer, Taiyuan in both summer and autumn, and Shanghai in the whole year. As for other countries, it was higher than that in the pristine site of Kumasi in summer and Malaysia in the whole year, while it was lower than that in Ulsan, the city center of Kumasi in summer, and Kathmandu Valley in the whole year.

3.2. Source Identification

PAHs molecular diagnostic ratios have long been used as a tool for PAHs source identification purposes [5, 26, 29–32], whereas some molecular diagnostic ratios were suggested to be of limited use as a source identification tool [33, 34]. Comparisons of diagnostic ratio results among Nanjing and other regions are presented in Table 3.

Table 3: Comparisons among diagnostic ratios found in this study with other urban areas in the world.

Region
Sampling time
Object
Diagnostic ratios
References

ANT/(ANT + PHE)
BaA/(BaA + CHR)
FLA/(FLA + PYR)
IcdP/(IcdP + BghiP)
BaP/BghiP
FLO/(FLO + PYR)

Nanjing, China
Summer, 2015
PM₁₀
0.12
0.43
0.53
0.51
1.06
0.56
This study

Jilin, China
Year, 2012
PM₁₀
0.16
0.37
—
—
1.47
—
[35]

Beijing, China
Summer, 2006
PM₁₀
0.11
0.27
0.46
0.20
—
—
[18]

Xuzhou, China
Summer and autumn, 2010
PM₁₀
0.57
—
—
0.66
0.54
0.52
[36]

Xuzhou, China
Winter, 2010
PM₁₀
0.44
—
—
0.56
1.16
0.52
[36]

Xi’an, China
Summer, 2009
Air
0.08 ± 0.04
0.29 ± 0.07
0.61 ± 0.01
0.55 ± 0.01
—
—
[37]

Shenzhen, China
Summer, 2010
PM₁₀
0.26
—
—
0.48
0.42
—
[38]

Taichung, Taiwan, China
Years 2002-2003
Air
—
—
—
0.55
—
0.55
[39]

Giza, Egypt
Summer, 2007
Air
0.46
0.39
0.61
0.35
0.77
—
[40]

Dourados, Brazil
Year, 2010
Air
—
0.34 ± 0.07
—
—
0.88 ± 0.31
—
[29]

Kuala Lumpur, Malaysia
Spring, 2011
PM₁₀
0.13
0.36
0.43
0.23
0.27
—
[30]

Delhi, India
Years 2009-2010
PM₁₀
—
0.61 ± 0.05
0.53 ± 0.07
0.30 ± 0.12
1.10 ± 0.51
—
[41]

Years 2001–2003
Air
—
—
—
—
—
0.71
[8]

BS: background site.

For the ratio of ANT/(ANT + PHE) (Figure 2(a)), the values were all greater than 0.1, indicating combustion sources. Likewise, for the ratio of BaA/(BaA + CHR) (Figure 2(a)), the values were all greater than 0.35, indicating combustion processes. The above ratios both indicated combustion process. All kinds of combustion processes were common sources of particle PAHs, being similar with the results in Jilin, China, in the whole year, in Xuzhou, China, in summer, autumn, and winter, in Shenzhen, China, in summer, in Giza, Egypt, in summer, and in Kuala Lumpur, Malaysia, in spring (Table 3). Combustion can be viewed as the major source of the atmospheric PAHs in summer in Nanjing.

(a)

(b)

(c)

Figure 2: Scatter diagrams for diagnostic ratios.

For the ratio of FLA/(FLA + PYR) (Figure 2(b)), the values were all greater than 0.5, indicating biomass and coal combustion. For the ratio of IcdP/(IcdP + BghiP) (Figure 2(b)), the values ranged from 0.31 to 0.60 with a mean of 0.51, primarily indicating biomass and coal combustion, along with petroleum combustion. The above ratios primarily indicated fuel combustion. Biomass and coal combustion is common in many developing regions (Table 3).

For the ratio of BaP/BghiP (Figure 2(c)), the values were all greater than 0.6, indicating traffic exhaust. The outcome may be explained by the results of FLA/(FLA + PYR) as traffic exhaust originates mainly from fuel combustion. With the development of traffic transportation, traffic exhaust has become a significant source of atmospheric PAHs (Table 3). For the ratio of FLO/(FLO + PYR) (Figure 2(c)), the values ranged from 0.35 to 0.64 with a mean of 0.56, primarily indicating diesel vehicle emissions with gasoline vehicle emissions attached. The outcome was similar with that in Xuzhou, China, in summer, autumn, and winter, Taichung, Taiwan, China, in the whole year, and Flanders, Belgium, in the whole year (Table 3). The above ratios indicated traffic exhaust, especially diesel vehicle emissions.

To summarize, atmospheric PAHs in PM₁₀ in summer in Nanjing originated mainly from combustion processes. Among these, vehicle emissions were outstanding, especially diesel vehicle emissions.

3.3. Risk Assessment

The cumulative probability distributions of the calculated inhalation exposure to PAHs for diverse population groups in Nanjing are shown in Figure 3. In summer, the median concentrations of inhalation exposure in Nanjing were 10.72, 15.43, 22.43, 14.19, 10.44, 14.86, 16.82, and 12.87 ng/d for boys, male adolescents, male adults, male seniors, girls, female adolescents, female adults, and female seniors, respectively (Figure 3). The rankings of exposure levels according to age and gender were adults > adolescents > seniors > children and males > females, respectively, in accordance with the rankings of inhalation rate for all age and gender groups, respectively.

(a)

(b)

Figure 3: Probability distributions of daily inhalation exposure in PM₁₀ in summer in Nanjing.

Researches concerning daily PAHs inhalation exposure level are limited. The median concentrations of inhalation exposure to total atmospheric PAHs in Taiwan, China, were 1590 and 1628 ng/d for children and adults, respectively. The data were much greater than the corresponding ones in this study as the samples in Taiwan contained 21 PAH compounds [13]. The median concentrations of inhalation exposure to total atmospheric PAHs in Taiyuan, China, for diverse population groups were 81.7 ng/d to 265.0 ng/d [14], still being higher than the results in this study resulting from serious PAHs pollution in Taiyuan. The mean concentrations of inhalation exposure to total atmospheric PAHs in outdoor environments of Tianjin, China, were found to be 321.6 and 519.0 ng/d for children and adults, respectively, being much higher than the results in this study, resulting from serious PAHs pollution in Tianjin [42]. All the comparisons indicated that the daily inhalation exposure levels in Nanjing were relatively low.

The cumulative probability distributions of the calculated ILCR for different population groups in Nanjing in summer are presented in Figure 4. The median values of ILCR were estimated to be , , , , , , , and for boys, male adolescents, male adults, male seniors, girls, female adolescents, female adults, and female seniors, respectively (Figure 4). According to USEPA, the acceptable level of risk is less than one in a million chance of additional human cancer over a 70-year lifetime (ILCR = 10⁻⁶), whereas the level of risk which is one in ten thousand or greater (ILCR = 10⁻⁴) is considered serious and in this case more attention should be paid to the health problems. The median values of ILCR in Nanjing in summer were in the acceptable level. ILCR values at 84.02th, 87.42th, 59.88th, 87.32th, 83.40th, 87.06th, 61.18th, and 86.20th percentile for boys, girls, male adolescents, female adolescents, male adults, female adults, male seniors, and female seniors, respectively, were greater than 10⁻⁶ (Figure 4), indicating low potential carcinogenic risk. ILCR values were larger than 10⁻⁴ at 99.92th, 99.98th, 99.2th, 99.96th, 99.96th, 99.98th, 99.4th, and 99.98th percentile for the above groups, respectively (Figure 4), indicating significant cancer risk. With respect to age, the ranking of ILCR in decreasing order was as follows: adults, children, seniors, and adolescents for both males and females. The greatest ILCR values for adults resulted from the greatest daily inhalation exposure levels and exposure duration, despite their highest body weight. It is worth noting that although the daily inhalation exposure levels and exposure duration of children were the lowest, the lowest body weight of children made its ILCR values greater than those for both adolescents and seniors. Children are sensitive to health risk and more attention should be paid. According to gender, females showed higher cancer risk in all age groups except the adults (Figure 4), which was not in accordance with exposure results (Figure 4). Although the exposure of females was less than that of males (Figure 4), the body weight of females was much lower, resulting in higher ILCR value (Figure 4).

(a)

(b)

Figure 4: Cumulative probability of respiratory incremental life time cancer risk for population groups in Nanjing in summer.

Researches on lung cancer risk assessment for PAHs are limited. The mean values of ILCR caused by inhalation exposure to PM₁₀-bound PAHs were 7.23 and for adults and children in Amritsar, India [43], being greater than the corresponding values in this study. The mean value of ILCR caused by PM₁₀-bound PAHs was estimated to be for general citizens in an urban area of Kuala Lumpur, Malaysia [30], being higher than almost all groups in this study (Figure 4). The median values of ILCR caused by 16 PAHs in suburban area of Shanghai, China, were , , , , , and for girls, female adolescents, female adults, child boys, male adolescents, and male adults, respectively [44], being a litter larger than the corresponding values in this study (Figure 4). The median values of ILCR caused by total PAHs in urban districts of Taiyuan, China, an industrial city, were estimated to be , , , , , , , and for boys, male adolescents, male adults, male seniors, girls, female adolescents, female adults, and female seniors, respectively [14], also being larger than the corresponding values in this study (Figure 4). Therefore, ILCR for residents in Nanjing was in a low level compared with results from regions worldwide.

4. Conclusion

The mean concentration of PM₁₀-bound PAHs in summer in Nanjing was much less than the Chinese National Standard, whereas it was higher than the European Union standard, indicating PAHs pollution. Atmospheric PAHs in summer in Nanjing originated mainly from combustion processes, in which traffic exhaust (especially diesel vehicle emissions) contributed a lot. The rankings of inhalation exposure levels according to age and gender was adults > adolescents > seniors > children and males > females, respectively, in accordance with the rankings of inhalation rate for all age and gender groups, respectively. The median values of ILCR in Nanjing in summer were in the acceptable level. ILCR values for adults were greater than those for other age groups, indicating that adults might be the main objects in the process of lung cancer risk control. Children were sensitive to health risks of environmental pollution.

Competing Interests

The authors declare that there are no competing interests.

Acknowledgments

This study was jointly supported by the National Natural Science Foundation of China (41001344), National Program on Key Basic Research Project (2014CB953802), China Postdoctoral Science Foundation Funded Project (2013M541696), Jiangsu Planned Projects for Postdoctoral Research Funds (1301040C), Program of Natural Science Research of Jiangsu Higher Education Institutions of China (13KJB610008), Program of State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences (SKLECRA2013OFP07), Program of Graduate Education Reform and Practice of Nanjing Normal University (1812000002A521), Scientific Research Foundation of the High-Level Personnel of Nanjing Normal University (2012105XGQ0102), Training Program of Innovation and Entrepreneurship for Undergraduates in Nanjing Normal University, and the Priority Academic Program Development of Jiangsu Higher Education Institutions (164320H116).

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