Enhanced Quenching Effect of Neonicotinoid Pesticides on Time-Resolved Terbium Luminescence in Presence of Surfactants

A luminescence analytical method for the quantification of neonicotinoid pesticides (acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, thiacloprid, and thiamethoxam) in water samples is proposed. ,e method is based on the quenching effect that these analytes produce on the time-resolved luminescence signal of terbium ions (excitation/emission wavelengths of 256/545 nm/nm). An enhancement on the analytical signal is observed when surfactants are used in the system. ,e use of a 0.01mol·L sodium dodecyl sulfate produced approximately a 30% increase in the sensitivity of the method. Under the optimum conditions, the method presented detection limits between 0.03 and 0.23 μg·mL. Interference studies were performed for ions commonly present in waters, observing no interferences. In addition, recovery experiments were performed in different waters, well, spring, and swamp, observing recovery yields close to 100%, with relative standard deviations lower than 4% in all cases. No sample treatment except a filtration step was required. Hence, this method may represent an alternative to other existing methods for the quick and simple analysis of these ubiquitous insecticides in environmental waters.


Introduction
Neonicotinoids are broad-spectrum systemic insecticides that are used in hundreds of field crops throughout the world.e use of these insecticides has constantly grown since their appearance.In 1990, organophosphates dominated the market, but by 2008, neonicotinoids already represented a 24% share of the pesticides market [1], and they still are the most abundant pesticides.Neonicotinoids are persistent, have high leaching and runoff potential, and are toxic to many invertebrates, hence representing a risk to surface waters, fauna, and ecosystems [2].Five neonicotinoids are approved as active substances in the European Union (EU) for plant protection products: acetamiprid (AC), clothianidin (CL), imidacloprid (IM), thiacloprid (TH), and thiamethoxam (TX).On the other hand, dinotefuran (DF) and nitenpyram (NT), which are the most recent neonicotinoids, are not approved in the EU [3].All neonicotinoids are potent agonists, acting selectively on insect nicotinic acetylcholine receptors [4].
Most of the concerns regarding the use of neonicotinoids are related to their harmful effects to bees, which also affect whole ecosystems [5].Hence, the European Union prohibited the use of CL, IM, and TX on crops attractive to bees, and these chemicals will be banned on outdoor crops [6].Although more controls have been demanded in crops, the use of neonicotinoids also derives in the contamination of environmental waters close to agricultural areas [7], which can produce adverse effects to additional nontarget species.For instance, the presence of residues of IM in surface waters in the Netherlands has been related to a decrease in the abundance of aquatic macroinvertebrate species [8].
is study was consistent with previous reports concerning the effects of neonicotinoids in aquatic invertebrates [9,10].erefore, it is important to control the levels of these pesticides not only in crops but also in environmental waters.
In this work, we propose a novel luminescence method for the quantification of neonicotinoids in environmental waters.
ese pesticides do not present native fluorescence, so other alternatives are required for their luminescent determination.An interesting approach is the use of luminescence probes, recording the variation observed by the addition of the analytes.Here, we report the quenching produced by neonicotinoids on the luminescence signal of terbium.In analytical chemistry, the detection called terbium-sensitized luminescence (TSL) is based on the increase observed when terbium ions form complexes with fluorescence compounds [25].However, in the method proposed here, the analytical signal is the quenching produced by the analytes on terbium ions. is method makes use of one of the most important advantages of TSL, the long luminescence lifetime of terbium luminescence, which allows performing time-resolved luminescence measurements, thus avoiding interference from fluorescence compounds [26].In addition, we report the enhancement observed in the analytical signal when surfactants are used in the system.e analytical method proposed here was successfully applied to the individual quantitation of seven neonicotinoids in different environmental waters.
e calibration graphs were prepared in the linear dynamic ranges and are shown in Table 1.Emission luminescence spectra were recorded at an excitation wavelength of 256 nm, and the peak height of 545 nm was measured.e quenching observed in the analytical signal by the addition of each analyte was the analytical signal (terbium signal minus terbium + analyte signal).Hence, a terbium blank solution was always recorded.e instrumental conditions were excitation and emission slit widths, 20/20 nm/nm; photomultiplier voltage, 750 V; delay time, 0.1 ms; gate time, 3 ms.All standard and sample solutions were analyzed in triplicate.

Results and Discussion
Neonicotinoids are nonfluorescent or slightly fluorescent compounds.Hence, native fluorescence cannot be measured, and photochemically induced fluorescence is the most usual detection technique for their spectroscopic quantification.However, we observed that neonicotinoids produce a quenching effect on the time-resolved luminescence of lanthanide ions.
e quenching was proportional to the analyte concentration (each analyte was tested individually), so the instrumental and chemical variables were optimized to obtain the highest sensitivity.e use of terbium instead of europium resulted in approximately a 30-50% enhancement in the analytical signal, so terbium ions were used for further experiments.

Instrumental Variables.
e usual approach for analytical methods based on terbium luminescence consists in the use of terbium-sensitized luminescence (TSL).However, TSL is suitable for fluorescence compounds that form complexes with terbium ions, increasing the luminescence intensity.e detection proposed here is based on the opposite effect: the quenching of terbium luminescence by the analytes.erefore, both excitation and emission wavelengths corresponded to terbium, resulting in optimum excitation/emission wavelengths of 256/545 nm/nm.e luminescence signal was recorded in the timeresolved mode.We thus optimized the delay (0.1-0.3 ms) and gate (1-5 ms) times.e analytical signal decreased as the delay time increased, so we set it at 0.1 ms.On the other hand, the analytical signal increased up to 3 ms of gate time, which was the selected value.
Finally, the effect of the excitation/emission slit widths (5-20 nm) and the voltage of the photomultiplier tube detector (400-800 V) were studied.Taking into account that the analytical signal corresponded to the quenching produced by the analyte on terbium ions, these parameters were optimized to obtain the highest terbium signal (blank signal) 2 Journal of Chemistry to improve both sensitivity and linear dynamic range.e highest the terbium blank signal, the highest the sensitivity of the system.e highest analytical signal was obtained for excitation/emission slit widths of 20/20 nm/nm and a photomultiplier voltage of 770 V.

Chemical
Variables.e pH value, as well as terbium and surfactant concentrations, was optimized in this section.First, the pH of the sample or standard solutions is a critical parameter, affecting the interaction between terbium ions and the analytes.We studied the influence of the pH between 3.0 and 8.0, adjusting the pH of solutions with NaOH or HCl.In most of the terbium-based luminescence analytical methods, the use of a pH value in the range 5.0-6.0 produces the highest analytical signal.In this work, pH values between 4.5 and 6.0 also produced the highest sensitivity.ere was no quenching for pH values lower than 4.0, whereas the use of pH values higher than 7.5 produced terbium hydrolysis and could not be used.Hence, we tested the influence of two buffer solutions (acetate and citrate) in the optimum pH range, obtaining the best results for an acetate/acetic acid buffer solution at pH 5.5 and a concentration of 0.05 mol•L −1 .
Secondly, we optimized terbium concentration, which was a critical parameter.e effect of terbium was studied for concentrations between 10 −5 and 10 −3 mol•L −1 .
e signal increased proportionally to terbium concentration up to a value of 5 × 10 −4 mol•L −1 , which was chosen as optimum for all the analytes (higher terbium concentrations did not improve the analytical signal).
Finally, we studied the influence of different surfactants, cetyltrimethylammonium chloride, SDS, and Triton X-100, on the analytical signal.e use of surfactants is common in TSL, as they can cancel the quenching of coordinated water molecules and protect the chelate against nonradiative processes [25].In this case, we measure the quenching of the signal; hence, it is important to avoid other deactivating mechanisms in the luminescence signal.Of the studied surfactants, only SDS produced an improvement on the analytical signal.is is probably due to the interaction between terbium and SDS micelles, whereas no interaction with the other micelles took place.e effect of SDS concentration was tested between 0.001 and 0.015 mol•L −1 , obtaining the best signal at a 0.01 mol•L −1 SDS concentration for all the analytes (Figure 1), the enhancement depended on the analyte structure and, consequently, on its potential to interact with the terbium-SDS system.As it can be observed in Figure 2, NT sensitivity was highly improved (more than 50% enhancement), whereas CL signal was barely affected.However, improvements around 30% were observed for most of the analytes.
We evaluated the quenching mechanism through the Stern-Volmer equation.is equation is applicable if the experimental results show linear variation, which was not the case for the target analytes.We observed a positive variation of the linearity, hence indicating that the quenching mechanism is not only collisional [27].

Interference Study.
We studied the potential interference of different ions that can be present in environmental waters.
is study was carried out for 1 µg•mL −1 for each analyte, observing similar results in all cases.e tolerance level was defined as the level of foreign species that produced an error lower than ±4% (the usual repeatability) in the determination of the analytes.As an example, the results obtained for NT are shown in Table 2. e tolerances observed indicate that these analytes could be quantified in the target samples (Figure 3) without interferences from other inorganic ions, although a sample treatment would be required for sea water or some groundwaters that may present higher ions concentrations.In addition, time-resolved luminescence is used as detection technique.Hence, other coexisting fluorescent pesticides would not interfere due to  the time-resolved mode employed, which avoids the recording of fluorescent background signals.

Figures of Merit.
e analytical parameters of the method were studied under the optimum conditions previously described.Although chemical and instrumental variables were the same for all the analytes, differences were observed in terms of sensitivity.Figures of merits are shown in Table 1 for all the analytes.e calibration graphs were constructed fitting the data by standard least-squares treatment, whereas detection and quantitation limits were calculated by the 3σ and 10σ criteria.
e lowest detection limit was obtained for TX (0.03 µg•mL −1 ), whereas the highest detection limits corresponded to CL and IM (0.23 µg•mL −1 ).Repeatability was lower than 4%, whereas intermediate precision (3 consecutive days) was lower than 5% in all cases.
e robustness of the method was also evaluated by performing small variations in instrumental variables (±2 nm in excitation/emission wavelengths; ±10 V in photomultiplier tube) and chemical variables (±5% variation in terbium, buffer, and SDS concentrations).In all cases, the variations of the analytical signal were lower than 4% compared to the optimum conditions.

Analytical Applications.
e method developed was applied to the determination of neonicotinoids in environmental water samples: spring, swamp, and well.Four samples, located in different locations in the province of Jaén (South of Spain), were selected.First, all samples were analyzed (after filtration to eliminate particulate matter) to ensure the absence of the analytes.Taking into account that neither of the analytes was present in the selected samples, we performed recovery experiments in all samples.Each sample was spiked with each analyte at three different concentrations (1000, 2000, and 4000 µg•L −1 ).No matrix effect was observed, so we used external calibration.Taking into account that four water samples were spiked with six analytes at three concentration levels, the average results obtained for all the analytes at each concentration are shown in Figure 3 to summarize the results.In all cases, recoveries between 90 and 110% and relative standard deviations (n � 3) lower than 4% were obtained.To evaluate the accuracy of the method, we used the method of the average recovery [28], which demonstrated the accuracy of the analytical method.

Conclusions
In this work, we report a novel analytical method for the quantitation of neonicotinoid insecticides.e method is based on the quenching effect produced by the target analytes on the luminescence signal of terbium ions.e use of time-resolved luminescence permits to avoid interferences from other potentially present fluorescence compounds.In addition, the enhancement produced in the analytical signal by the use of the surfactant SDS has been reported.We validated the accuracy of the method by recovery experiments in four environmental waters, obtaining recovery yields close to 100% in all cases.Hence, this method may represent a simple and rapid alternative to other existing methods, although positive results would have to be    Journal of Chemistry analyzed by liquid chromatography to assess the exact identity of the neonicotinoid pesticide detected.

Figure 1 :
Figure 1: Effect of sodium dodecyl sulfate on the analytical signal for 10 mg•L −1 nitenpyram.

Figure 3 :Figure
Figure 3: Mean recovery (average of all the analytes) for each water sample at three different spiked levels.

Table 1 :
Analytical parameters for all the studied analytes.