Simultaneous Determination of Sunset Yellow FCF , Allura Red AC , Quinoline Yellow WS , and Tartrazine in Food Samples by RP-HPLC

An efficient method was developed for the simultaneous determination of Sunset Yellow FCF (E110), Allura Red AC (E129), Quinoline Yellow WS (E104), and Tartrazine (E102) in food samples by RP-HPLC. &e mentioned food dyes were analyzed at room temperature for 23min with gradient elution. &ree mobile phases were used for the elution, and mobile phase A was an acetate buffer (pH � 7.5, 1%), mobile phase B was acetonitrile, and mobile phase C was methanol. &e flow rate was 1.0mLmin, and the injection volume was 20 μL. &e linear ranges were 0.72–50mg L, 0.24–50mg L, 0.75–10mg L, and 0.69–50mg L for Tartrazine, Quinoline YellowWS, Sunset Yellow FCF, and Allura Red AC, respectively. R values were 0.999 for all dyes. Limits of detection were 0.24mg L, 0.08mg L, 0.25mg L, and 0.23mg L for Tartrazine, Quinoline YellowWS, Sunset Yellow FCF, and Allura Red AC, respectively. &e relative standard deviation (RSD) of the measurements for all of the four dyes was between 0.56 and 1.65% intraday measurements. &is method was successfully applied in the determination of the mentioned dyes in ice pops, gummy bears, chewing gum, and sweets candy samples.


Introduction
Synthetic food dyes are legally used in order to increase the attractiveness and color of the food [1].Other than natural dyes, synthetic dyes are also used as additives in foodstuffs within legal boundaries.Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine are synthetic food dyes that are widely used in ice cream, popsicles, confectionery, gummy bears, juices, bakery, meat, sweets candy, desserts, snacks, drinks, and sauces [2,3].It has been thought that these food dyes cause some adverse effects, especially on the nervous system [4][5][6].e health problems related to the synthetic food dyes depending on the reactions of the aromatic azo compounds in the structure are attention deficit hyperactivity disorder, allergies, food intolerance, and asthma [6][7][8].e toxicity of the food dyes on the living organisms has been investigated [9][10][11].Many countries have banned and strictly controlled the uses of these dyes in foodstuff and drinks [12].Some spectrophotometric methods have been proposed for the analysis of food dyes [2,3,[13][14][15].However, mostly high-performance liquid chromatography methods for determining synthetic food dyes have been used.In these methods, the chromatographic conditions in which many dyes can be simultaneously determined have been investigated [16][17][18][19][20].In particular, it is desirable that the determination time is short, and the operations before the determination are simple and not time-consuming.It is preferred that the mobile phase contains less amount of organic solvent in terms of environment.For this reason, there is still a need to develop a simple, fast, and environmentally friendly new methods.
Spectrophotometric methods are simple but usually require enrichment procedures before measurements [3].
HPLC methods are the most widely used methods due to their high sensitivity.However, the extraction of the dye and the chromatographic process can be time-consuming.In the present study, the development of a RP-HPLC method for the simultaneous determination of Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine in many types of foods has been aimed.erefore, a faster and more environmentally friendly method of determination is proposed for the simultaneous determination of four food dyes using less chemical than those of literature methods.
e four synthetic food dyes are efficiently separated using an optimized gradient elution in a single run within 15.1 min.e proposed method is validated in different food matrices, such as ice pops, gummy bears, chewing gum, and sweets candy, which is successfully used in the analysis of commercial samples.
e HPLC grade methanol (JT Baker) and acetonitrile (Merck) were used.Water was purified using an ultrapure water purification system (Sartorius Stedim Arium Pro UV).
Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine were purchased from Sigma-Aldrich (Steinheim, Germany).e structures, names, and C.I. numbers of the used dyes in this study are given in Scheme 1.
e stock dye solutions (1000 mg L −1 ) were prepared in water.Working solutions (50 mg L −1 ) were prepared freshly from the stock solutions by dilution with water.
e mobile phase A consists of an aqueous ammonium acetate solution 1% (m/v), brought to pH 7.5 by drop-wise addition of a sodium hydroxide solution 10% (m/v) (m/v: mass/volume).Acetonitrile and methanol were used as mobile phases B and C, respectively.
Ice pops, gummy bears, chewing gum, and sweets candy were purchased from local supermarkets in Trabzon, Turkey.

Apparatus. Chromatographic analysis was carried out
with an Agilent 1100 liquid chromatograph system integrated with a degasser Agilent 1260 and a UV detector.A pH meter (Eutech pH 510) equipped with a combined glasscalomel electrode was employed for pH measurements.An ultrasonic bath (Isolab Laborgerate GmbH) was used for the pretreatment of solid food samples.

Chromatographic Conditions.
A Zorbax Eclipse R XDB-C8 Analytical column (4.6 × 150 mm 5 micron, Agilent) was used as the column.e mobile phase A was filtered through a membrane filter with a pore diameter of 0.45 μm.For optimization, a number of gradient elution programs were tested by gradually decreasing the amount of the organic solvent.e optimum flow rate of eluent was 1.0 mL min −1 , and the injection volume was 20 μL. e optimized gradient program is shown in Table 1.All experiments were performed at room temperature.
e UV detector was used to detect the dyes at the specific wavelengths for each dye.ese wavelengths were 427, 417, 482, and 507 nm for Tartrazine, Quinoline Yellow WS, Sunset Yellow FCF, and Allura Red AC, respectively.
e chromatographic system was initially conditioned by passing the mobile phase A through the column for about 1 h to obtain a stable baseline signal.

Preparation of Dye Standards and Sample Solutions.
Individual standard stock solutions (1000 mg L −1 ) containing each dye were prepared by dissolving appropriate amount of dye in 25 mL distilled deionized water.e solutions were kept in dark flasks.e working standard solutions of each dye were prepared by appropriate dilution of stock solutions with water to give concentrations between 0.10 and 50 mg L −1 .
e mixed standard solutions containing all dyes at concentrations between 1.0 and 50 mg L −1 were also prepared by mixing and dilution of appropriate aliquots from standard stock solution of each substance.All solutions were stored at 4 °C in the dark and were stable at least for 2 months.
Gummy bear samples (10.0 g) in water (75 mL) were shaken to dissolve for 25 min in an ultrasonic bath, and the volume was made up to 100 mL in a volumetric flask.Sweets candy samples (20.0 g) were homogenized with a blender.e 4 g homogenized sample in water (75 mL) was shaken for 25 min in an ultrasonic bath at 25 °C.e solution was filtered through a folded paper filter and then through 0.45 μm disposable syringe filter.e filtrate was collected in a volumetric flask of 100 mL.e sweets candy extract was filtered only through 0.45 μm disposable syringe filters.Chewing gum samples (20 g) in water (75 mL) were shaken in an ultrasonic bath for 15 min at room temperature.e solution was filtered through 0.45 μm disposable syringe filter.
e filtrate was collected in the volumetric flask of 100 mL.Ice pop samples (10 g) were dissolved in water (75 mL).en, they were filtered through 0.45 μm disposable syringe filters.e solution volumes were completed to 100 mL in the volumetric flask.

Validation of the Method.
In order to determine the linear region of each dye, the measurements were carried out using different concentrations among 0.10-50 mg L −1 for all compounds, with three replicates per concentration.Calibration curves were also prepared with the mixed standard solutions at concentration levels of 1.0, 2.5, 5.0, 10.0, 25.0, and 50.0 mg L −1 to check the selectivity of the method.e calibration curve of each dye was used for the validation of experiments and determination.
e limit of detection (LOD) (3× Sd/m) and the limit of quantification (LOQ) (9× Sd/m) were determined by using the residual standard deviation of the regression line (Sd) and the slope of the calibration line (m) [21].To verify the accuracy of the proposed method, analysis of the same food samples was carried out by using a standard method [22].
e Allura red amount in the gummy bear found using the standard method NMKL 130 was not statistically different from those of the proposed method.e results are given in Table 2. Statistical analysis of the results was made using Student's t-test, and there was no significant difference 2 Journal of Chemistry between the results.e experimental t-values were below the critical t values (2.78, significant level � 0.05).erefore, matrix components were not expected to interfere in the determination of the food dyes, Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine.

Optimization of the Separation.
In this study, a simple reversed-phase liquid chromatography (RP-LC) method to determine simultaneously four synthetic food dyes, Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine, in various foodstuffs is developed.e hydrophobicity of these dyes depends on the presence of the azo group and the quinoline ring.Normally, the retention time of the polar molecules is shorter than that of apolar molecules under reverse-phase liquid chromatography conditions [19].However, the presence of acidic and alkaline groups in the compounds changes the elution sequence.Usually, acetonitrile and methanol were used to elute these types of food dyes in the literature [19,20].e use of a modifier is a common approach to separate ionic species in short analysis time.An inorganic electrolyte as a modifier is generally added to the mobile phase [19].For this aim, acetic acid-ammonium acetate buffer (pH 5-7.5) has been recently used as a modifier for the purification and separation of many azo dyes by RP-HPLC [19,20,[23][24][25]]. e ammonium acetate concentration higher than 0.1 M was not suitable for water-soluble food dyes because of the "salting-out" effect  Journal of Chemistry [26].at the acetate buffer solution as a modifier does not interact with the HPLC system and consistence for UV absorbance measurements are also advantages.e optimum pH was determined to be 7.5 for the mobile phase A because there are some examples of successful separation of water-soluble food dyes at this pH in RP-HPLC systems [19,[27][28][29][30].
A series of mixed standard solution (1, 2.5, 5.0, 10, 25, and 50 mg L −1 ) containing the four water-soluble dyes was used to study the optimum conditions of separation.Many gradient elution programs were tested to obtain the shortest analysis time.e most suitable program was the gradient program shown in Table 1.
e chromatogram of the mixture of four dyes (individual concentration 1 mg L −1 ) is shown in Figure 1. e peaks and their corresponding t R are shown in Table 3.As seen from Figure 1 and Table 3, there are three peaks for the mixture of Quinoline Yellow WS, and the t R values of these peaks are 8.7, 14.6, and 15.1 min.ese peaks are observed due to isomeric structures of Quinoline Yellow WS dye [22].4 gives LOD, dynamic ranges (limit of quantification-limit of linearity (LOQ-LOL)), calibration equations of mixed standard dye solutions, and coefficients of determination (R 2 ).e peak area   of the compounds was used to calculate calibration equations.e dynamic ranges for the dyes are different from that of each other.e LOL value is 50 mg L −1 for all dyes, except for Sunset Yellow FCF.For this dye, the shorter linear range is obtained.e linearity limit is 10 mg L −1 for Sunset Yellow FCF.Detection limits for all dyes are nearly same (between 0.23 and 0.25 mg L −1 ), except for Quinoline Yellow WS with 0.08 mg L −1 .e detection limit for Quinoline Yellow WS in Table 4 belongs to Quinoline Yellow WS I isomer.

Validation of the Method. Table
Both the intra-and interday precisions were below 5% at a concentration level of 1.0 mg kg −1 for food matrices chosen, in case of all dyes.

Application to Real Samples.
e method developed was applied to foodstuffs samples purchased from local market and supermarkets.e preparation of samples was given in Section 2.4.Ice pops, a sample of gummy bears, three samples of chewing gum, and two samples of sweets candy were analyzed (n � 3), and the results are summarized in Table 2.Both Allura Red AC and Tartrazine were detected in sweets candy I sample.All results were below the legal limits [31].

Figure 1 :
Figure 1: Chromatogram of a mixed standard dye solution by using the optimized gradient program in this study.Individual dye concentration was 1 mg L −1 .

Table 1 :
Optimized gradient program for the separation of four dyes by HPLC-UV, at a flow rate of 1.0 mL min −1 .

Table 2 :
Determination of the synthetic dyes in food samples (N � 3).

Table 3 :
Chromatographic data of the dyes with the optimized gradient program in this study.