Bioadsorption of Basic Blue Dye from Aqueous Solution onto Raw and Modified Waste Ash as Economical Alternative Bioadsorbent

Background. Dyes are one of the most hazardous materials in industrial effluents which can cause several health problems in living organisms. The removal of dye from colored effluents has attracted increasing attention in the last decade. In this study, raw, beneficiated, and activated waste ash were evaluated as adsorbents for removal of methylene blue (MB) from aqueous solution by the batch adsorption method. Comprehensive characterization studies were carried out on each bioadsorbent, such as proximate analyses, bulk density, specific surface area, point of zero charge, pH, and Fourier transform infrared (FTIR) spectroscopy (which shows functional groups on adsorbents surface). The effects of pH, adsorbent dosage, initial dye concentration, and contact time were determined in order to know the optimum condition and adsorption potential of the adsorbents. The methylene blue (MB) removal efficiency of raw, beneficiated, and activated bioadsorbents from aqueous solutions was found to be 95.212%, 89.172%, and 84.504%, respectively. It is reported that adsorption efficiency of MB on each adsorbent was quite different due to electrostatic and dispersion interaction between the dye molecules and the surface property of the adsorbents. The obtained results were well fitted with the Freundlich isotherm model, and the adsorption process follows the pseudo-second-order kinetics model for all adsorbents. In fact, the results showed that raw, beneficiated, and activated waste ash bioadsorbents could be employed as effective and economical alternative material in the near future.


Background
Many manufacturing industries such as textile, paper, plastics, cosmetics, leather, and food use dyes for coloring their products [1]. About 50% of the total world production of dyes goes off during the dyeing process and has been released in textile effluents [2]. ose considerable amounts of dyes are lost and discharged into water streams, which ultimately affects the aquatic life. In addition, the colored effluents are known to be carcinogenic and highly toxic to living things in contact by different mechanisms [3]. Among those dyes, methylene blue is one of the basic (cationic) and heterocyclic aromatic compounds. It has many applications which include coloring paper and dyeing textile products (wool, silk, cotton), in addition to its use in leather industries. In spite of its several applications, this dye has a number of negative impacts (if swallowed or made in contact with skin or eye) on human beings and animals, such as irritation of mouth, throat, and stomach with symptoms of nausea, vomiting, shock, diarrhea, increased heartbeat, cyanosis, jaundice, and tissue necrosis in humans [1,4]. Undesirable dye pollution is required to be removed from wastewater before being discharged to the environment. Several physical and chemical treatment methods such as adsorption, oxidation, biodegradation, chemical coagulation/flocculation, and chemical precipitation have been used for the treatment of wastewater containing dyes [4][5][6]. Adsorption is one of the physiochemical methods found to be the most simple and economical to remove the dyes from effluents [7]. Several adsorbents have been reported in the literature such as ground eggshell waste [8], modified silica gel [9], tobacco steam ash [10], rice hull ash [11], tea waste [12], coffee grounds [13], fishery waste [14], coconut shell activated carbon [15], agricultural waste products [16], Jatropha curcas pods [17], vegetable residues [18], calcined bones [19], waste bamboo culms [20], and orange and banana peel [21].
Removal of harmful dyes from effluents is an economic fashion that remains a major problem for textile industries [5]. However, high capacity low cost adsorbents are still under development to remove/minimize textile effluents. Waste ash is a leading waste in Woldia town, Ethiopia, which may be thought of as other inexpensive materials used as adsorbents. It has been preferred as it is an easily available waste material mainly coming from hotels, student cafes, and other household activities and stored in a common waste disposal area. It is obtained from plant stems, and charcoal for fuel/thermal energy purposes generates waste ash with no known economic uses. e main plant source used for such purposes is Girar (Acacia bussei). e aim of this work is to study the bioadsorption potential of waste ash for the removal of harmful methylene blue from aqueous solution.

Preparation of Bioadsorbents.
Waste ash, white in color and noncombustible residue, was obtained from the waste disposal area of Woldia town, Ethiopia, as shown in Figure 1.
e waste ash used in this experiment was prepared without any treatment (raw) and modified with distilled water (beneficiated) and 1 NH 2 SO 4 (activated) as a ratio of 1 : 5 with continuous stirring for 24 hr. e sample mixtures have been filtered and washed several times with distilled water. Filtered waste ash samples were dried at 105°C for 24 hr, powdered by mortar until the samples were equally fine, sieved in the size of 200 μm, and kept in desiccators for further use.

Preparation of Basic Blue Dye (MB) Solution.
Basic blue dye (cationic dye) was taken from Kombolcha (S. Wollo) Textile Share Company, Amhara region, Ethiopia. Basic blue dye is powdered solid, soluble in water; it has molecular weight of 319.9 g/mol and maximum absorption wavelength of 665 nm; its chemical formula is C 16 H 18 ClN 3 S; and its IUPAC name is 3,7-bis(dimethylamino)-phenothiazin-5ium chloride. e stock solution (500 mg/L) of the dye was prepared, and the required concentrations were obtained by serial dilution of the stock solution. e maximum wavelength of the dye (665 nm) was obtained after full scanning using UV/Vis spectrophotometer (Lambda 35 Perkin Elmer). A linear calibration curve was obtained with different concentrations of methylene blue dye solution as shown in Figure 2.

Physiochemical Analysis.
e properties of the adsorbents such as moisture content, ash content, volatile matter, fixed carbon, and bulk density were determined according to [22][23][24][25] with slight modifications.
Moisture content: about 1 g of each adsorbent sample was weighed and placed in a clean, dried, weighed, and placed in crucible in a preheated oven at 105°C for 4 hr. en, the samples were cooled, kept in desiccators at room temperature, and measured again. e moisture content of the adsorbents was obtained using where M 0 and M 1 are the initial and final mass (g) of the adsorbents, respectively. Ash content: about 1 g of each adsorbent sample was placed in crucibles, weighted, and heated in a muffle furnace (Nabertherm B180) under a temperature of 500°C for 4 h. After that, the crucibles containing samples were allowed to cool in a desiccator to room temperature and reweighed. e ash content of samples was calculated using where Ma and Ms are the mass (g) of the adsorbents and ashes, respectively. Volatile matter: 1 g of each adsorbent sample was placed in predried crucibles and heated in a muffle furnace regulated at 500°C for 8 min. en, the crucibles were cooled in desiccators and weighed. Finally, the volatile matter of the adsorbents was calculated using where M 1 and M 2 are initial mass (g) and final mass (after drying) of adsorbents, respectively. Fixed carbon: fixed carbon content of adsorbents was determined by subtracting the percentage of MC, AC, and VM using fixed carbon content(FC)% � 100% − (MC + AC + VM)%.

(4)
Bulk density: the mass to volume ratio of adsorbents was used to determine the bulk density of the adsorbents. A measuring cylinder (10 mL volume/size) was weighted. Sufficient amount of the adsorbents was poured into it with constant tapping and filled to the 10 mL mark. e difference between the initial weight of the container/measuring cylinder and the final weight (container + sample) was calculated.
e mass difference was taken as the mass of the adsorbents. en, the bulk density was calculated from the relationship of mass to volume and determined using bulk density g mL � mass(g)of the adsorbent volume(mL)of the container .
Surface area determination: the specific surface area of the adsorbents was estimated according to Sears' method [26][27][28]. About 1.5 g of raw, beneficiated, and activated waste ash was mixed with 30 g NaCl and dissolved by 100 mL of distilled water using 250 mL conical flask. e mixtures 2 Journal of Chemistry were stirred for five minutes. en, the pH of each solution was adjusted to 4, and the solutions were titrated by 0.1 M NaOH until pH of the solution reaches 9. e volumes of NaOH required to change pH value from 4 to 9 were recorded. e specific surface area of each sample was obtained using the following formula: where V is the volume of 0.1 M NaOH required to raise the pH from 4.0 to 9.0. Determination of pH: raw, beneficiated, and activated waste ash, about 1 g each, were mixed in 100 mL of distilled water and continuously stirred for 2 hr. e samples were allowed to stabilize before the measurement, and the supernatants were analyzed for pH using pH meter [24,29].
Determination of point of zero charge (PZC): the surface property (surface charge) of the adsorbents was determined by measuring the pH of zero point charge [30] of raw, beneficiated, and activated waste ash. For the determination of pHPZC, 0.01 M NaCl was prepared and its initial pH was adjusted between 2 and 12 by using 1 M NaOH and 1 M HCl in each batch. en, 50 mL of 0.01 M NaCl was taken into 250 mL Erlenmeyer flasks, and 0.20 g of each adsorbent was added to the solutions. ese flasks were kept for 24 h, and the pH values of the solutions were measured by using a pH meter. e results were then plotted between final pH and initial pH. e point of intersection of the curves of "final pH versus initial pH" is the PZC of adsorbents.

FTIR Analysis.
e surface functional groups of the bioadsorbents were studied by FTIR spectroscopy (JASCO model 4100). e analyzed samples were prepared by mixing 1 mg of each dried adsorbents with 500 mg of KBr (spectroscopic grade) in an agate mortar and then pressing the resulting mixture in order to form pellets. en, FTIR spectra were recorded between 4000 and 400 cm − 1 .

Batch Adsorption Experiment.
Adsorption experiments were carried out in the batch mode by optimizing different parameters: solution pH (4-13), contact time (20-120 min), adsorbent dosage (0.1-2 g), and initial dye concentration (25-100 mg/L). A measure amount of dye sample (50 mL) and 1.6 g of adsorbent were taken into 250 mL conical flask for batch experiment and agitated with magnetic stirrer on digital hot plate at 200 rpm. e initial pH values of all the solutions were adjusted with 1M HCl or 1M NaOH. At the end of each experiment, small amounts of the solutions (supernatants) were withdrawn at predetermined time, and the absorbance was determined using UV/Vis spectrophotometer at a maximum wave length of 665 nm. e adsorption capacity at equilibrium, the methylene blue dye removal (%), and q e (mg/g) are shown in the following equations [25,31,32]: where C 0 is the initial dye concentration (mg/L), C e is the liquid-phase concentrations of the dye (mg/L) at equilibrium, V is the volume of the dye solution (L), and m is the mass of the adsorbents (g).

Physiochemical Characteristics.
Physiochemical characteristics of the adsorbents such as moisture content, ash content, volatile matter, fixed carbon, bulk density, solution pH, and point of zero charge were determined and are presented in Table 1. e raw and modified (beneficiated and activated) waste ash had almost similar low moisture contents, which indicates that the adsorption of moisture by the samples is also low. e moisture content has no significant effect on adsorptive power unless it increases the weight and hinders the porous nature of adsorbents [33]. e volatile matter contents were found to increase significantly from raw and beneficiated to activated waste ash (raw < beneficiated < activated). During activation step, organic molecules present in adsorbents become unstable; volatile matter is released as gas and liquid products which evaporate leaving a material [24,31]; and also significant amounts of mineral matter, carbonates, certain oxides, and sulfides are removed [34]. e result of volatile matter of adsorbents revealed that the active sites of the adsorbents might be removed in the case of activation. e ash contents were also significantly increased from raw and beneficiated to activated waste ashes, causing a decrease in adsorptive properties of adsorbates. A good adsorbent must have low ash content [35]. Ash content can lead to increased hydrophilicity and can have catalytic effects, causing restructuring process during regeneration of used adsorbents [30,34]. e fixed carbon contents were found to decrease significantly from raw and beneficiated to activated waste ashes. is could be attributed to the higher competition between activators (water and sulfuric acid) and carbon as well as other elements present in the adsorbent to release more volatile components with concomitant improvement in the textural characteristics and carbon burn-off [23]. e bulk densities were decreased from raw and beneficiated to activated waste ashes. High bulk density is considered to be a good quality of the adsorbent materials [22]. e surface area of raw and modified waste ashes was determined according to Sears' method. Activated waste ash was found to have a low surface area (935 m 2 /g) than beneficiated (1319 m 2 /g) and raw (1465 m 2 /g) waste ashes. e reduced value was due to the high concentration of H 2 SO 4 that attenuated the pore volume and the surface area [31]. e surface area of activated ash was also reduced due to the presence of significant amount of ash because the presence of these inorganics fills or blocks some of the existing networks of micropores [34] and it occupies some of the open pores of the adsorbent material [36]. e results showed that water treatment also affects the surface area of the waste; this may be due the presence of high ash content compared to the raw material. In addition; water treatment may alter the surface of waste ash through protonation and deprotonation.
e pH of raw, beneficiated, and activated waste ashes was 11.65, 11.35, and 8.9, respectively. e results confirmed that the waste ash adsorbents are basic in nature, pH > 7. e point of zero charge (PZC) was determined for both raw and modified waste ashes and is presented in Figure 3. It was found to be 10, 10, and 8.2 for raw, beneficiated, and activated waste ashes, respectively. When the pH of the solution is below the PZC, the surface of the adsorbent will become positively charged, and when the solution pH is greater than PZC, the surface of the adsorbent will become negatively charged [25]. is implies that the raw, beneficiated, and activated waste ashes most probably adsorb methylene blue dye on basic regions.

FTIR Analysis of Adsorbents.
FTIR spectra of the raw, beneficiated, and activated waste ashes are presented in Figure 4. e results show that waste ash adsorbents have many different functional groups due to different adsorption band areas. e intensity of absorption peaks was significantly increased from raw and beneficiated to activated waste ash due to modification of the surface of the adsorbents with water and sulfuric acid. e spectra showed broad band at a range of 3550-3400 cm − 1 confirming the presence of the hydroxyl (O-H) groups of carboxylic acids, alcohols, phenols, and adsorbed water on the surface of adsorbents [37,38]. e band at a range of 1615-1430 cm − 1 is attributed to C�C stretching vibrations, and the peak <900 cm − 1 results of C-H (out of plane) confirm the presence of aromatic ring in the adsorbent [34]. e absorption peaks at a range of 1130-1040 cm − 1 are attributed to the C-O stretching vibrations.

Effects of Experimental Conditions on Adsorption
Efficiency. Effects of solution pH, adsorbent dosage, initial dye concentration, and contact time have been investigated individually for determination of ideal experimental conditions under room temperature.

Effect of Solution pH.
Removal efficiency of the adsorbents has been strongly affected by the pH of the solution in adsorption studies [25]. e effect of initial pH on adsorption of methylene blue dye by raw, beneficiated, and activated waste ashes was studied in pH range between 4 and 13, dye concentration of 25 mg/L, and adsorbent dose of 0.4 g at room temperature, as presented in Figures 5(a) and 5(b). e maximum dye removal by raw, beneficiated, and activated waste ashes was 2.101 mg/g (67.244%) at pH 8, 1.824 mg/g (58.368%) at pH 12, and 1.417 mg/g (45.328) at pH 12, respectively. e fact that the maximum adsorption of methylene blue was high at higher pH (at basic region) indicates that the surface of each adsorbent has become negatively charged. erefore, the mechanism of dye removal was explained via the electrostatic interaction between the negatively charged surface of the adsorbents and the positively charged methylene blue dye adsorbate [39,40].

Effect of Adsorbent Dose.
e increase in adsorbent dosage was found to increase the removal efficiency of methylene blue until equilibrium was reached and is Journal of Chemistry presented in Figure 6(a). e methylene blue (MB) removal efficiencies of raw, beneficiated, and activated from aqueous solutions were 95.212%, 89.172%, and 84.504%, respectively, at optimum dosage value of 1.6 g. ereafter, no significant change has been observed in all adsorbents, because adsorption saturation was reached or due to overlapping of   Journal of Chemistry 5 adsorption sites as a result of overcrowding of adsorbent particles. As shown in Figure 6(b), the adsorption capacity of adsorbents was found to decrease with increasing the adsorbent dosage, which might be due to the interaction of adsorbent particles like aggregation or agglomeration which results in decrease of the total surface area [4].

Effect of Initial Concentration of Dye.
Effects of initial concentration of dye have been examined by using different dye concentrations from 25 to 100 mg/L, as described in Figures 7(a) and 7(b). e adsorption capacity increased with increasing the concentration of dye from 0.744 to 2.833 mg/g for raw, 0.697 to 2.732 mg/g for beneficiated, and 0.66 to 2.479 mg/g for activated bioadsorbents. On the other hand, the percentage of removal efficiency of the dye was decreased from 95.212% to 62.564% for raw, 89.172 to 49.7% for beneficiated, and 84.504% to 17.247% for activated waste ashes with increasing the initial dye concentration. At a lower initial concentration of methylene blue, there will be unoccupied active sites on the adsorbent surface, and when the initial dye concentration increases, the active sites required for adsorption of the dye molecules will be occupied or saturation of active bioadsorbent sites will be reached [25].

Effect of Contact Time.
To examine the minimum time required for maximum performance of adsorbent process, the contact time was varied from 20 to 120 min at optimum values of adsorbent dose of 1.6 g, pH of 4 for raw and 12 for beneficiated and activated adsorbents, and initial dye concentration of 25 mg/L at room temperature. As shown in Figures 8(a) and 8(b), it has been observed that the removal efficiency and adsorption capacity increase with the increase in contact time up to 100 min, and beyond this time there is no more change. In fact most vacant surface sites are available for adsorption during the initial stage, and the remaining vacant surface sites are hard to adsorb due to repulsive forces between the dye molecules on the adsorbents and the bulk phase.

Kinetics Study.
e kinetic experiments were conducted at the optimal conditions: pH � 8 for raw and pH � 12 for beneficiated and activated waste ashes, adsorbent dose 1.6 g, initial dye concentration 25 mg/L, and contact time 20-100 minutes for all adsorbents. Kinetic models have been exploited to test the experimental data and to find the mechanism of adsorption and its potential rate-controlling step that includes mass transport and chemical reaction [41]. In addition, information on the kinetics of dye uptake is required to select the optimum conditions for full scale batch or continuous dye removal processes. Adsorption kinetics is expressed as the solute removal rate that controls the residence time of the sorbate in the solid-solution interface. Mostly used models including the pseudo-first-order and pseudo-second-order ones were applied to the experimental data to evaluate the bioadsorption kinetics of methylene blue dye. e applicability of these kinetic models was determined by measuring the correlation coefficients (R 2 ) and the experimental q e value [39,40]. In order to further understand the characteristics of the adsorption process, the pseudo-first-order and pseudo-second-order kinetic models were applied to fit experimental data obtained from batch experiments and are presented in Figures 9 and 10, respectively.
e pseudo-first-order kinetic models are expressed in linear form as follows: where q e is the amount of adsorbate adsorbed at equilibrium (mg/g), q t is the amount of solute adsorbed per unit weight of adsorbent at time (mg/g), and k 1 is the rate constant of pseudo-first-order sorption (1/hr) [25]. e values of k 1 and q e were calculated from the slope and intercept of log (q e − q t ) vs t graph, respectively, and the results are presented in Table 2 for all adsorbents. e result of pseudo-second-order model is shown in Figure 10 and expressed in (10) in linearized form as follows: where k 2 (g·mg − 1 ·min − 1 ) is the rate constant of the pseudosecond-order adsorption, q e is the amount of dye adsorbed on the adsorbent at equilibrium (mg/g), and q t is the amount of dye adsorbed on the adsorbent at any time, t (mg/g). e values of k 2 and q e were calculated from slope and intercept of t/q t vs t graph, respectively. e kinetic parameters and the correlation coefficients (R 2 ) were determined from nonlinear regression. Figures 9  and 10 represent pseudo-1 st and -2 nd order kinetics, respectively, and their parameters are presented in Table 2.
e calculated q e values (q e , cal) of pseudo-2 nd order model are close to the experimental ones (q e , exp) as compared to pseudo-1 st order kinetics model. e R 2 values also for the pseudo-second-order kinetic model are close to 1, which indicates that experimental data were in good agreement with pseudo-second kinetics order model.

Adsorption Isotherms.
In order to study how the molecules of MB interact with the adsorbent surface, the adsorption isotherms were used to analyze the experimental data. Isotherms study can describe how an adsorbate interacts with an adsorbent [42,43]. e isotherm provides a relationship between the concentration of dye in solution and the amount of dye adsorbed on the solid phase when both phases are in equilibrium. e most widely used isotherm equations are Langmuir and Freundlich equations. ese two isotherms have their own assumptions that explain the adsorption mechanism, and they are presented in Figures 11 and 12, respectively. e Langmuir model is used to describe the formation of monolayer adsorbate on the outer surface of the adsorbent. It is often applicable to a homogeneous adsorption surface with all the adsorption sites having equal adsorbate affinity, while Freundlich isotherm assumes that during the adsorption process different sites of the adsorbent with several adsorption energies are involved, which is an empirical relation for adsorption over heterogeneous surfaces. e linear expression of Langmuir isotherm model can be illustrated as follows: where C e is the equilibrium concentration of solute (mmol L − 1 ), q e is the amount of solute adsorbed per unit weight of adsorbent (mmol·g − 1 of adsorbate), q m is the adsorption capacity (mmol·g − 1 ), or monolayer capacity, and b is a constant (L·mmol − 1 ). e isotherm parameters b and q m were determined from the intercept and the slope of C e /q e versus C e graph, respectively. e linear expression of Freundlich isotherm model can be illustrated as follows: where K f and n are empirical constants incorporating all parameters affecting the adsorption process such as sorption capacity and sorption intensity, respectively [44]. e isotherm parameters K f and n were calculated from the log q e vs log C e graph and are presented in Table 3. For the Freundlich isotherm if n = 1, then the partition between the two phases is independent of the concentration. If value of 1/n is below one, it indicates a normal adsorption. On the other hand, 1/n being above one indicates cooperative adsorption. For this study, as shown in Table 3, the values of 1/n were below 1 for all adsorbents, which confirms that adsorption of MB on these adsorbents was normal adsorption. e essential characteristics of the Langmuir isotherm can be expressed in terms of a dimensionless constant separation factor R L given by the relation in (13) that can be used to determine the feasibility of adsorption in a given concentration range over adsorbent [41,45,46]: where b is Langmuir constant related to the energy of adsorption (L·mg − 1 ) and C 0 is initial concentration (mg/L). e calculated R L values at different initial dye concentrations are listed in Table 4. R L value indicates the adsorption nature to be either unfavorable if R L > 1, linear if R L � 1, favorable if   [46]. For this study, all the values lied between 0 and 1, confirming that the adsorption of dye over the adsorbent was favorable. All isotherm parameters for both isotherms are presented in Table 4, and the R 2 values of Freundlich isotherm are greater than those of Langmuir model for all adsorbents, which confirms that Freundlich isotherm is suitable for describing the adsorption equilibrium of MB onto these adsorbents.

Conclusion
In summary, this experimental study showed that the removal of methylene blue through adsorption on raw, beneficiated, and activated waste ash adsorbents is an effective, economical, safe, and eco-friendly alternative. e adsorption potential obtained using raw waste ash is higher than that using the same dose of beneficiated and activated bioadsorbents. In fact water and sulfuric acid treatment causes a significant reduction in the total surface area, enhancement of ash content, and reduction of fixed carbon and promotes the formation of acidic surface groups via protonation. e adsorption kinetics was in good agreement with pseudo-second-order kinetic model, and the adsorption isotherm showed good fitting to Freundlich isotherm model.
is study clearly shows that adsorption strongly depends on solution pH, adsorbent dose, dye concentration, and contact time of the experimental parameters.

FTIR:
Fourier transform infrared spectroscopy UV/Vis: Ultraviolet visible spectroscopy PZC: Point of zero charge AC: Ash content MC: Moisture content VM: Volatile matter FC: Fixed carbon.

Data Availability
All data generated or analyzed during this study are included within the article.

Conflicts of Interest
e authors declare that they have no conflicts of interest.

Authors' Contributions
All the authors contributed to experimental work, data analysis, and manuscript writing, editing, and final approval.