Degradation Pattern of Textile Effluent by Using Bio and Sono Chemical Reactor

The research study was conducted to design the ultrasonic-assisted electrochemical reactor and the bioreactor/filter to evaluate the potential applicability of biological trickling filter system and to compare the efficiency of two reactors such as SER and TF for the treatment of textile industry effluents. Also the study to design Sonoelectrolytic process for wastewater treatment of textile industry containing strong color, high temperature, suspended particles and dissolved solid particles has been conducted. Effect to environment and health is caused by oxygen demand (BOD), high chemical oxygen demand (COD). The percentage removal efficiency for wastewater treatment of textile industry by using sonoelectrolytic reactor (SER) was found to be higher than 95% at temperature of 25°C and a pH value of 8.9, while for trickling filter (TF), having adsorbent as a filter medium, efficiency was found to be 95%, and optimum conditions obtained were applied for the treatment of different dye samples. Based on experimental outcomes, it is determined that treatment through SER is done faster than trickling filter because in TF the adsorbent capacity decreases with time and is a time-consuming process, but the chance of deposition on electrodes also increases in SER, so both these processes can yield better results if these problems are eliminated.


Introduction
In the last few years, wastewater from the textile industry has become a big problem which causes an increase in the concentration of environmental pollution in industrial cities, which in turn represents environmental risks [1]. Released wastewater in the textile industry and the chemical dyes can be considered as an important environmental concern, as about 200 L of water is used for every 1 kg of textile production [2]. Textile dyes contain a significant quantity of organic pollutants which are very difficult to degrade, and yearly around 5,000 tons of coloring materials are discharged into our environment [3]. e quantity of used water and the waste production both depend upon the amount of consumed water by several varieties of fabrics, and it changes from one textile industry to another due to reliance on the dyeing process. A rough estimation shows that the bleaching process requires 38% of water, printing requires 8%, dyeing requires 16%, boiler requires 14%, and 24% is required for additional practices [4]. As a result of diverse processes, an enormous amount of hurtful effluents is discharged to the environment. us, textile effluent has a significant bit of colors and further destructive synthetic concoctions that are dangerous to the environment and are a long way from fulfilling the standards.
Textile industry effluent contains large number of harmful agents such as chemical oxygen demand (COD), biological oxygen demand (BOD), suspended particles, dissolved solid particles, chemicals, color and metals (zinc (Zn), arsenic (As), copper (Cu) and, chromium (Cr)) which causes harm to humans and environment [5]. Coloring release contains a composite blend of colors, salts, and further synthetics, for example, surfactants and about 90% of the color and 80% of the salts are released in effluent [6]. Textile effluent is based upon manufacture products and chemicals and is classified in terms of acidic, basic, vat, and azo dyes while a variety of chemicals inculcated include detergents, caustic, latex, glues, and other chemicals [7]. Textile dyeing works utilizing various classes of chemical dye and extra synthetic substances produceing mixed wastewater [8]. e proficiency of the advanced oxidation process for degrading textile dyes containing the intractable compounds has been comprehensively acknowledged [9][10][11].
On estimation, most colors utilized in the textile business are effectively solvent in water and are nonbiodegradable due to their unyielding mixes and are a threat to the environment and could cause cancer [12]. e total input of dyes toward wastewater flow is about 15-20% and textile dyeing is contributing about 17%-20% of industrial discharge. ere are about 72 toxic chemicals that have been recognized in water from textile dyeing, 30 of which cannot be removed easily [13].
Intense coloration of textile dyes poses high risks to aquatic life by creating obstruction to the light path to reach underwater. Many colors present in fiber effluent potentially cause cancer and mutation and are genotoxic [14]. Material coloring process incorporates different activities, for example, pretreatment, coloring, printing, and washing of pieces of clothing resulting into the generation of a lot of contaminated effluents. e transformation of fiber up to one ton produces about 230-270 cubic meter of polluted water and is required to be cleaned before releasing into the environment [15]. Textile effluents as colors are the most dangerous compound blends found in fiber effluents and ought to be dealt with splendidly as their appearance in water bodies diminishes light invasion, blocking the photosynthesis of watery verdure [16,17]. Photo-Chemical and ozone chemical treatment processes are collectively resulted as the quick degradation process with better efficiency but the expense of this method is high and is not powerful for treating the entire azo dyes. Also, the generation of various radicals with the expansion of substance reagents causes minor drop of TOC and COD qualities creating the lackluster however degradable polluted water [18].
Industrial dyes involve aromatic compounds in their chemical composition. ese dyes are synthesized by chemical combination of altered functional group and relocated electrons. e chromogen comprises a fragrant structure regularly relying upon benzene, naphthalene, or anthracene. e chromophore arrangements are, the azo gathering (-N=N-), ethylene gathering (=C=C=), methine bunch (-CH=), carbonyl gathering (=C=O) and chinoid gatherings. Azo colors might be poisonous after metabolic decrease of the azo bond, creating sweetsmelling amines. e auxo chrome bunches are ionizable gatherings that present coupling limit to the dyematerial. e standard auxo chrome bunches are-NH 2 (amino), -COOH (carboxyl),-SO 3 H (sulphonate), and -OH (hydroxyl) [19]. Dyes are classified as natural and synthetic based upon application characteristics and chemical structure. Different treatment techniques of textile wastewater have been proposed in the literature [20].
Fabric industry is perhaps the biggest business on the planet, and various textures, for example, silk, cotton, and fleece are all prerestored, handled, and hued, utilizing a lot of water and a range of chemicals after treatment, so there is a need to comprehend the textile effluent well overall.

Classification of Dye Removal Techniques.
ere are several dye removal techniques ( Figure 1) which are classified as chemical, physical, and biological methods. Physical method for removing dyes includes adsorption, ion exchange, and filtration/coagulation methods [21,22], while chemical methods include ozonisation, Fenton reagent, and photocatalytic reactions and biological methods include aerobic degradation, anaerobic degradation, biosorption, etc.

Fenton Oxidation Process.
Propelled oxidation strategies, for example, Fenton and altered Fenton forms are impressive to notice dye removal in textilewastewater. In Fenton process, low concentrations of Fe +2 and H 2 O 2 arrangements are utilized, and these Fenton reagents create OH-radicals including high oxidation potential. e Fenton procedure, where nonlethal and innocuous reagents are applied at low concentrations, is extremely helpful for decolorization of wastewater since it is exceptionally viable and less dirty [23].

Membrane-Based Technologies.
Conventional treatment methods experience the ill effects of a few loopholes. e utilization of film-based procedures in such cases can viably surmount a large portion of these disadvantages. e choice of suitable membrane relies upon the layer material which is thus administered by certain indispensable layer properties, for example, chemical, mechanical thermal and the film defenselessness to fouling; in addition, the layer pore size, which decides the substances that can be adequately held and layer shape, which demonstrates its capability to oppose stopping up are other significant parameters that must be thought about [24,25]. Microfiltration has constrained application in textile wastewater treatment due to its resemblence with filtration process. It is mainly used for removal of particlessuspension and colloidal dyes from exhausted dye bath and from discarded rinsing bath discharge; microfiltration membranes, however, permit the unconsumed auxiliary chemicals, dissolved organic pollutants and other soluble contaminants to escape with the permeate [25,26]. e ultrafiltration layer process has restricted applications in the textile business; this is mostly in light of the fact that the subatomic loads of the colors present in the exceptionally hued material release are a lot of lower than the subatomic weight cut-off (MWCO) of the ultrafiltration membranes [27]. Ultrafiltration (UF) is generally applied as a pretreatment step in frameworks requesting high level of procedure stream refinement; it is trailed by procedures, for example, nanofiltration (NF), or invert assimilation (RO) stages, which fulfill the requests on process water quality [28]. Nanofiltration (NF) layer process is distinctively put between ultrafiltration and reverse osmosis, Its popularity over the years as an effective and simplified technology can be attributed to the benefits in terms of environmental pollution abatement, rejection, recovery and reuse of textile dyes, divalent salts and other auxiliary chemicals, recovery and reuse of brine . Furthermore, the generation of value pervade permits the reuse of treated wastewaters in significant procedures, for example, coloring and wrapping up [29].

Adsorption Technique.
Adsorption methods have picked up support methodology among all the physicochemical techniques including adsorption, flocculation joined with flotation, membrane filtration, electromotor, coagulation, ozonation, oxidation, precipitation, and ion exchange [30,31]. Activated carbon is a generally utilized adsorbent in modern procedures, which is made out of a miniaturized scale permeable, homogenous structure with high surface territory and shows radiation security [32]. ese days, there is an incredible enthusiasm for finding economical and powerful options in contrast to the current business activated carbon [33]. e most recent investigation shows the readiness of activated carbon from coconut husk with H 2 SO 4 actuation (CSAC) and its capacity to evacuate textile colors (maxilon blue GRL and direct yellow DY 12) from fluid arrangements [34]. Agricultural wastes are inexhaustible, accessible in huge sums and more affordable when contrasted with different materials utilized as adsorbents. Agricultural squanders are superior to different adsorbents in light of the fact that the horticultural squanders are typically utilized without or with at least handling (washing, drying, and granulating) treatment [35].
ere are explicit option farming by-products utilized strongly as color adsorbents, for example, nut structure, coir essence, and rice husk [36].

Microbial Biotechnology.
Biotechnological methodologies have pulled in overall consideration for their relative cost viability and ecologically well-disposed nature. Most biotechnological approaches depend on the utilization of organisms that can possibly enzymatically debase and decolorize color containing fiber effluents. Azo dyes have been seen as decolorized prevalently under anaerobic conditions. Complete deterioration of azo dyes by microbial cells happens in two stages. In the initial step, drab metabolites are delivered by the reductive cleavage of the azo bond under anaerobic conditions. ese metabolites are then disintegrated in a second step that requires oxidized conditions [37]. Enzymes, for example, azo reductases, laccases, lignin peroxidases, Mn peroxidases, DCIP-NADH reductases, tyrosinase, aminopyrine N-demethylase, and riboflavin reductases, have been accounted for to be engaged with the breakdown of azo dyes [36,38,39].

Ozonation.
Biotreated fabric wastewater can be more dangerous than the untreated discharge as certain colors are changed into little natural atoms; in this manner, further treatment is mostly required. Especially encouraging are the advance oxidation forms (AOPs); among the few AOP ozonation is the innovation that can be effectively executed in the previously existing treatment plants.operate [40,41]. Utilization of ozonation as short posttreatment after a natural procedure can be gainful for the breakdown of refractory mixes and the evacuation of poisonous quality of material wastewater, yet observing of mutagenicity and harmfulness is a significant apparatus and ought to be utilized to supplement ordinary examination which centers around expulsion of supplements [42]. 2.6. Phytoremediation. Phytoremediation really is the technique for treatment of contaminations by plants and their root related microflora [43]. Phytoremediation approach has been examined as a potential instrument to evacuate numerous risky natural contaminants like substantial metals, landfill leachates, pesticides, polyaromatic hydrocarbons, radionuclides, oil, unrefined petroleum, chlorinated solvents, polychlorinated biphenyls, explosives, weapons, and even the harmful gases [44]. Phytoremediation has advantages such as being a solar energy dependent and an aesthetically pleasant method of treatment. It offers a carbon neutral and thus environmental friendly approach for removal of toxic contaminants from the environment [45].

Instruments
3.1.1. pH Meter. pH meter is used for the measurement of acidity and alkalinity of solution. pH meter is used to measure the pH of wastewater at different time intervals during wastewater treatment process of industrial dye. is device is also considered as one of the easiest and devices used to measure pH; in addition, this device is equipped with standard solutions to ensure the inspection process for accurate results. Spectrophotometer was used to determine the concentration of dyes in wastewater at different time intervals during treatment process, and it can be explained by using a chart diagram.

Electrolytic Cell.
Electrolytic cell is an undivided cell made of Perspex sheet in which degradation was carried out. Working electrode (anode) was made of copper and counter electrode (cathode) was made of aluminum plated iron. Both electrodes had dimensions of 15 × 4 cm 2 placed vertically at distance of 10 cm to each other. Figure 2 produces high energy sound waves. e frequency of these waves ranges from 10-400 kHz. ese sound waves help in wastewater treatment as they transfer their energy to pollutant molecule. Due to their high energy, the pollutant molecule ruptured.

Sonoelectrolytic Reactor (SER).
It is the combination of two units, i.e., an electrolytic cell and ultrasonic bath. e electrolytic cell was placed in the ultrasonic bath, and both are operated simultaneously for wastewater treatment as explained in Figure 2.

Experimentation
e wastewater was treated with two systems. e experimentation of our study consists of three steps: (1) Sonoelectrolytic reactor.

Treatment through Sonoelectrolytic Reactor (SER)
4.1.1. Experiment No. 1. 50 ppm concentration of solution of dye colored red was prepared. e volume of solution was 2 L. e underlying pH, temperature, and absorbance were noted, and the arrangement was placed in sonoelectrolytic reactor. e treatment process continued for 210 minutes. e time interval was 30 minutes. Different parameters like absorbance, pH, and temperature were determined after every 30 min of interval.

Experiment No. 2 (Acidic Solution)
. 50 ppm concentration of red dye solution was prepared. One milliliter for each liter of concentrated HCl was incorporated to solution. Solution's color was changed from red to blue. e absorbance was now measured at wavelength of 570 nm. e volume of solution was 2 L. e initial pH, temperature, and absorbance were noted, and same procedure is repeated as discussed earlier.

Experiment No. 3 (Basic Solution)
. 50 ppm concentration of red dye solution was prepared. 0.1 N solution NaOH was added to solution. e absorbance was measured at wavelength of 495 nm. e volume of solution was 2 L. e initial pH, temperature, and absorbance were noted, and same procedure is repeated as discussed earlier.  and absorbance were noted, and same procedure is repeated as discussed earlier.

Adsorbent.
e adsorbent used was sugarcane bagasse.
e sugarcane leftover was collected from sugarcane juice shop and then dried in sunlight and ground into small size.

Bio-Trickling Filter (TF).
e bagasse adsorbent was used in the trickling filter. e laboratory scale trickling filter was constructed as shown in Figure 3. e volume of trickling filter was 4 liters. e filter media used were gravels and adsorbent layer. e filter depth was 8 cm. e bottom layer of filter was of gravels of 10 mm; this layer was of 5 cm. e second layer of 3 cm was laid of gravels having size of 2 mm. e upper layer of adsorbent was thin, approximately 2 mm. e quantity of adsorbent used was 24 gm. Two tests were performed on trickling filter.

Experiment
No. 1. 50 ppm concentration of solution of dye colored red was prepared. Volume of suspension was 4 L. e initial pH, temperature, and absorbance were noted, and same procedure is repeated as discussed earlier.

Experiment No. 2 (Collected Sample).
e sample was collected from cloth dyeing shop. e maximum absorbance that was measured by spectrophotometer was 675 nm. e volume of solution was 4 L. e initial pH, temperature, and absorbance were noted, and same procedure is repeated as discussed earlier. Table 1 are explained with the help of graphs (Figure 4(a)-4(c)). Table 1 shows the experiment implementation time and specified 210 minutes, where 8 samples were taken during different time every 30 minutes; the results showed (Figures 4(a)-4(c)) a decrease in the absorbance from 1.637 to 0.413 with an increase in the efficiency from 12.6% up to 99.3% at 210 minutes and equivalent pH values, which means that the processing time is 3.5 hours.

Experiment No. 1. Results shown in
Test continued for 210 minutes and 60 minutes settling was given as shown in Table 1. reactor. e pH also changes during treatment process. e max temperature reaches 61.5°C due to dye degradation because sound waves are highly energetic when they strike with atoms; the atoms are split into small particles which settle down during settling. Similarly, during electrolysis, movement of electrons also degrades dye atoms and emits energy due to this temperature increase. e efficiency was 99.3% after filtration as shown in Table 1. Table 2 shows the experiment implementation time and specified 90 minutes, where 4 samples were taken during different time every 30 minutes; the results showed ( Figure 5(a)-5(c)) a decrease in the absorbance from 1.154 to 0.059 with an increase in the efficiency from 13.7% up to 94.9% at 90 minutes, which means that the processing time is 1.5 hours.

Experiment No. 2 (Acidic Solution).
Test continued for 90 minutes and 60 minutes ettling time was provided.reactorDecrease in absorbance with time depicts the degradation of dye in solution within the reactor. e pH also changes during treatment process. e max temperature reaches 51°C. e efficiency was 94.9% after filtration. Table 3 shows the experiment implementation time and specified 210 minutes, where 4 samples were taken during different time every 30 minutes; the results showed (Figures 6(a)-6(c)) a decrease in the absorbance from 1.548 to 0.074 with an increase in the efficiency from 47.9% up to 95.2% at 210 minutes and high pH values, which means that the processing time is 1.5 hours.

Experiment No. 3 (Basic Solution).
Test continued for 90 minutes and 60 minutes settling was provided. reactorDecrease in absorbance with time depicts the degradation of dye in solution within the reactor. e pH also changes during treatment process. e max temperature reaches 52°C. e efficiency was 95.22% after filtration. Table 4 shows the experiment implementation time and specified 210 minutes, where 4 samples were taken during different time every 30 minutes; the results showed (Figures 7(a)-7(c)) a decrease in the absorbance from 3.824 to 0.138 with an increase in the efficiency from 1.15% up to 96.4% at 210 minutes, which means that the processing time is 1.5 hours.

Experiment No. 4 (Collected Sample).
Test continued for 90 minutes and 60 minutes settling time was provided.reactorDecrease in absorbance with time depicts the degradation of dye in solution within the reactor. e pH also changes during treatment process. e max temperature reaches 59°C. e efficiency was 96.4% after filtration. Figure 8 shows the initial and final absorbance of wastewater. is shows that SER is a preferable treatment process in short time period.

Drawbacks of Sonoelectrochemical Reactor.
e treatment efficiency of sonoelectrolytic reactor was very good. e only drawback assessed was deposition of dyes on electrodes during the treatment process that needs cleaning after every test. is depends upon the concentration of dyes in wastewater; higher concentration leads toward more deposition on electrodes. Another problem which may occur was sludge formation. High amount of sludge is produced in this process that is difficult to handle. Table 5 shows the experiment implementation time and specified 110 minutes, where 4 samples were taken during different time every 30 minutes; the results showed (Figures 9(a)-9(c)) a decrease in the absorbance from 1.591 to 0.067 with an increase in the      efficiency from 83.47% up to 95.78% at 110 minutes and equivalent pH values, which means that the processing time is 1.5 hours.

Experiment No. 1.
Test continued for 110 minutes. reactorDecrease in absorbance with time depicts the degradation of dye in solution within the reactor. e pH also changes during treatment process. e efficiency was 95.8%. Table 6 shows the experiment implementation time and specified 240 minutes, where 9 samples were taken during different time every 30 minutes; the results showed (Figures 10(a)-10(c)) a decrease in the absorbance from 3.79 to 2.02 with an increase in the efficiency from 20.95% up to 46.7% at 240 minutes and equivalent pH values, which means that the processing time is 4 hours.

Experiment No. 2 (Collected Sample).
Test continued for 110 minutes. reactorDecrease in absorbance with time depicts the degradation of dye in solution within the reactor. e pH also changes during treatment process. e efficiency was 46.7%.
e graph in Figure 11 shows the initial and final absorbance of wastewater. is shows that TF is not a good treatment process in short time period. Also the graph shows abrupt variation causing an increase in absorbance value as reaction stopped and particles deposited instead of settling deposited. e problem can be removed using filter paper.

Drawbacks of Bio-Trickling
Filter. Treatment in biotrickling filter is a time-consuming process as both the   Journal of Chemistry biological treatment and the physical treatment are required. Biofilm generation is a time requisite process, and the other issue was the capacity of adsorbent. e adsorbent capacity decreases with time, and when adsorbent surface area is utilized thoroughly, there is a need to replace the old adsorbent with the new one. Table 7 shows the comparative analysis of treatment systems by sonoelectrolytic reactor and trickling filter. Figure 12 shows the relevance between treatment efficiencies and time. It shows that sonoelectrolytic reactor (SER) took less time to treat textile wastewater as compared to trickling filter. Figures 13(a) and 13(b) show the temperature variation with respect to time for different experimental setup used in the ultrasound-assisted electrochemical reactor (UAER) method. It is observed that temperature increases as the experiment progresses. e absorbance decreases with increase of temperature, ultimately causing dye degradation to increase, respectively. e maximum temperature noted in UAER method is 56°C. Figure 13(b) shows the temperature variation for different experimental setup used in the sonoelectrochemical reactor (SER) method. e temperature shows the similar effect as observed in UEAR graph values. e maximum temperature noted in the SER method is 59°C. After 90 minutes, the experiment stops and the aqueous solution is kept for settling for 60 minutes, causing an abrupt decrease of temperature. Also, it is noted in both UEAR and SER methods that the rate of decolonization reduces after temperature reaches 50°C and rate of increase of temperature also decreases after 150 minutes in both the aforementioned techniques.    Wavelength (nm) Figure 11: Plot of absorbance with respect to wavelength over 830 nm. It shows the treatment efficiency of trickling filter.  Figure 12: Plot of efficiency with respect to time over 250 min period. It shows the efficiency comparison of SER and TF. Figure 14 shows the comparison between trickling filter, sonoelectrolytic reactor, and ultrasound-assisted electrochemical reactor based on their degradation efficiency.

Conclusion
Fabric discharge can cause drain, ulceration of skin, sickness, skin bothering, and dermatitis. Excessive quantity of harmful synthetic substances in the wastewater inhibits sunlight and enhances biological oxygen demand, thereby hindering photosynthesis and reoxygenation process. Hence, the need to treat such an effluent is urgent.
In this study, performances of sonoelectrolytic and biological processes to remove color and organic compounds from fabric discharge were investigated and compared. After performing a series of experiments on dye solution, it was easy to conclude that efficiency of degradation of dyes can be enhanced by changing the parameters like pH, temperature, and types of chemicals used. Conclusions derived from the experimental results shows that the treatment through SER is faster than trickling filter because in TF the adsorbent capacity decreases with time and is a time-consuming process, but the chance of deposition on electrodes also increases in SER so both these processes can yield better results if these problems are eliminated.

Data Availability
e data used to support the findings of the study are available from the corresponding author upon request.