Antimicrobial Activities of Some Constituents Isolated from a Kenyan Medicinal Plant, Capparis fascicularis DC

Background of the Study . Capparis fascicularis DC. is an indigenous medicinal plant belonging to the family Capparaceae found in Nyandarua County, Nairobi, Kenya, and many parts of Africa. It is a shrub whose roots are used traditionally to treat colds. Aim . Te aim of the present study is to carry out antimicrobial activities of solvent extracts of diferent parts of Capparis fascicularis , characterize the phytochemical constituents of the most active extract, and identify the most active compounds. Place and Duration of the Study . All the experiments were carried out in the departments of Chemistry and Microbiology, Kenyatta University, Nairobi, Kenya. Methodology . Petroleum ether, ethyl acetate, methanol, and water extracts from the leaves, stem bark, and root bark of C. fascicularis were screened against selected strains of both Gram-positive ( Staphylococcus aureus and Bacillus subtilis ) and Gram-negative ( Salmonella typhimurium and Escherichia coli ) bacteria using disc difusion and tube dilution methods. Fractionation of the most active crude extract was carried out by column chromatography, and the fractions together with the most active crude extract were screened against the selected bacterial strains. Te most active fraction was further fractionated, and the subfractions were screened against the bacterial strains to test for possible synergistic efects between the subfractions and their constituents. Te most active fraction was then analysed by GC-MS and LC-ESI-MS methods to identify the major constituents. Results . Te ethyl acetate extract of C. fascicularis root bark (CFR2) showed a signifcant in vitro antibacterial activity. From the seven fractions of CFR2 obtained, fraction 2 (F2) had the lowest MIC valueandwasthus mostactive.Moreover,F2wasfound tobemoreactive comparedtothe foursubfractionsobtainedfrom it. Tis suggested that constituents of F2 worked in synergy. Fraction 2 contained phenols, terpenes, and favonoids. Two compounds were identifed by GC-MS as 2,4-di-tert-butylphenol and ( E,E )-2,4-decadienal, while three compounds were identifed by LC-ESI-MS as tanshinone II A, cryptotanshinone, and danshensu. Conclusion . Te study revealed that CFR2 is the most active extract on bacteria, suggesting that most antimicrobial compounds are concentrated in the roots of C. fascicularis . A follow-up study is directed towards chromatographic separations to obtain the other chemical constituents and screen them against various strains of bacteria.


Introduction
One of the major challenges to the current drug administration against opportunistic infections is the increase in the number of drug-resistant pathogens.For instance, multidrug resistant tuberculosis (MDR-TB) requires treatment courses that are much longer and less efective than those for nonresistant TB [1].Te search for new pharmacologically active agents obtained by screening natural sources such as plant extracts has led to the discovery of many clinically useful drugs that play a major role in the treatment of human diseases.In essence, natural products or their derivatives form a large proportion of existing drugs and this could be due to their similar interactions with biological enzymes and therapeutic targets.Approximately, 60% of the antitumor and antiinfective agents that are commercially available or in late stages of clinical trials today are of natural product origin [2].Historically, the majority of natural product-based drugs such as cyclosporine, paclitaxel, and camptothecin derivatives were frst discovered by traditional cell-based in vitro assays (antibacterial, antifungal, antiviral, antiparasitic, or cytotoxic assays) before their real molecular targets were identifed [3].In the present study, we sought to screen for potential antibacterial activities of extracts of the roots of Capparis fascicularis DC and characterize their major phytochemicals.Te plant belongs to the family Capparaceae and is used traditionally to treat microbial infections.It is found in deciduous bushlands, riverine thickets, and bushed or wooded grassland.It is a climbing (20-40 M) shrub that is common in Kenya and many parts of tropical Africa in undisturbed forests.Te leaves are narrowly elliptic, base, cuneate to rounded, and slightly subcordate.Te leaf apex is puberulous (glabrous) [4].Flowers are white in several fowered axillary fascicles.Petals are 4-7 mm in length.Fruits are green to red, round, 1-2 cm across.Flowering occurs between January and February and between May and December [4].Tis shrub's roots are used traditionally to treat common cold [4,5].Previously, several studies carried out with diferent Capparis spp.have shown the presence of potentially useful phytochemical constituents.For example, the crude methanolic extract of the aerial and foral parts of Capparis decidua (Forssk) Edgew from Cholistan desert of Pakistan was found to be active against 13 bacterial strains (MIC 250-1000 μg/mL) [6].Likewise, a study with Capparis spinosa L. showed activity on Deinococcus radiophilus.In addition, when the decoction was added to a culture medium, the growth rate of D. radiophilus populations signifcantly decreased as compared to the control cultures [7].In another study, methanolic extract of Capparis sinaica Veill was tested for its in vitro antiviral activity against highly pathogenic avian infuenza strain H5N1 using the plaque inhibition assay in Madin-Darby canine kidney; the results indicated that it possessed potent antiviral activity (100% inhibition at the concentration of 1 mg/ml) [8].A study conducted on in vitro anticancer activity of stachydrine, which was isolated from Capparis decidua on prostate cancer cell lines, showed that it exhibited potential cytotoxic efects and there was a signifcant inhibition of expression of chemokine receptors.Te study also provided essential information about the antiinvasive and antimetastatic nature of the compound, prospecting it as a candidate for developing into potential anticancer therapeutics [9].

Methods.
Te leaves (1556.18g), root bark (6300 g), and stem bark (1427.25 g) of C. fascicularis (Capparaceae) were sampled in September, 2016, at South Kinangop in the central region of Kenya.Te identifcation was carried out by Mr. Lucas K. Karimi, a taxonomist at the Department of Pharmacy and Complementary Medicine, and the voucher specimen (No.MWK-2017/2) was deposited in the herbarium, Department of Botany, Kenyatta University, for future reference.Te plant parts after air-drying were ground, weighed, and extracted sequentially with (solvents of increasing polarity), that is, petroleum ether, ethyl acetate, and methanol.A fraction of the ground plant materials underwent aqueous extraction.Te extracts were concentrated in a rotary evaporator under reduced pressure and at temperature of about 45 °C.Te percentage yield for each extract is given in Table 1.Tey were then screened against selected bacterial strains [10].Aqueous extraction involved soaking of 100 g of the weighed plant material in 1000 mL of distilled water.Te mixture was boiled for thirty minutes in a conical fask and kept for 24 hours to allow for maximum extraction.Te extract was fltered using a flter paper and evaporated [10].A summary of the organic and aqueous extraction procedures for C. fascicularis plant parts is shown using Figure 1.

Antibacterial Screening.
Te disc difusion method was used to carry out tests on all extracts obtained from the plant against four bacterial strains (two Gram-negative strains; Escherichia coli ATCC 25922 and Salmonella typhi ATCC 33056, while two Gram-positive ones were Staphylococcus aureus ATCC 25923 and Bacillus Subtilis ATCC 6051 on the crude extracts of the plant at concentrations of 1 g/mL).Molten DST agar (15 mL) was dispensed into sterilized Petri dishes and then allowed to solidify.Tis was followed by inoculation of the solidifed agar with 2 drops (about 0.5 mL) of 10 8 CFUs/mL of the bacterial pathogen suspension in Muller Hinton broth (MHB) which was spread evenly on the surface of the agar [11].Sterilized flter paper discs of 6 mm diameter, previously soaked in 1 g/μL of the samples, were dried out and then aseptically mounted on the inoculated agar at eight divided sections on Petri dishes (Figure 1) using sterile forceps, slightly pressed and incubated at 37 °C for 24 hours for all the test bacteria.Te zone of growth inhibition was then observed and measured to the nearest millimeters after 24 hours of incubation.A reading of 6 mm was to indicate that either there was no activity at all or very little activity which was negligible for recording.A clear zone of inhibition around the discs was to indicate a positive reaction.Te zones were interpreted according to Clinical Laboratory and Standards Institute (CLSI) and indicated as susceptible, moderately resistant, or resistant.Each test was repeated three times.Tis replication of the data was to help serve as an internal quality check on how the experiment was performed [12,13].Te mean of the triplicate tests was calculated and recorded as the size of the zone of inhibition.Standard sensitivity discs of gentamicin were tested as positive controls.For plates that had clear inhibition zones, the area of inhibition was streaked and grown on another plate containing nutrient agar with no antibiotics.Tis was to observe whether there was any growth of microorganisms that would be realised.
Active plant extracts (showing zones of inhibition for diferent bacteria) were later tested at lower concentrations through serial dilutions to determine their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), using the microtitre broth dilution method [14].One disc was placed in each well.Petri dishes were half-flled with Muller Hinton agar (MHA) and then swabbed with the respective bacteria and labelled appropriately.Tis was done in triplicate.Tey were also divided into eight sections and labelled as per the dilutions.Te microtitre plates were divided into 4 sections along a row of 12 wells, each section having the names of the extracts in triplicate.Te discs from the wells were placed into Petri dishes accordingly.Tey were then placed in the incubator for 24 hours.Microtitre plates together with plates spread on nutrient agar were also used for the tube dilution method to measure the minimum bactericidal concentration (MBC).In each well of microtitre plates, 5 μL of bacterial broth suspension (10 8 CFU/mL) was placed.Tese microtitre plates were incubated for 24 hours.Using a wire loop, contents were drawn from the wells of incubated microtitre plates and streaked on the plates containing NA without antibiotics.Te streaked plates were incubated for 24 hours.Results were recorded where a tick (√) indicated the growth of microorganism, while (x) indicated there was no growth of microorganism on the streaked plate.
For NA in Petri dishes, 16 sections were drawn in these Petri dishes and labelled, for example, 1d, 1t; 2d, 2t; 3d, and 3t; where "d" represented the discs' sections of the inhibition zone which were streaked on the nutrient agar and "t" represented solution drawn from tubes of microtitre plates then streaked on NA contained in Petri dishes.Tese discs were also incubated for 24 hours.

Minimum Inhibitory Concentrations (MIC).
Active plant extracts were further tested by serial dilutions to determine their minimum inhibitory concentrations (MIC).Te results were tabulated and interpreted according to CLSI [15].

Minimum Bactericidal Concentrations (MBC).
Inoculation method was used to prepare Muller Hinton agar (MHA), DMSO, MHB, isotonic saline, and NA [14].Te wooden splints and cotton wool were used to make swabs, while aluminium foil was used to cover the swabs for sterilization.
Starting with an extract concentration of 0.5 g/mL, 100 μL of the dissolved extract was fetched using micropipette and put in the frst row of microtitre wells.In the second row, 50 μL of extract was placed, and then 50 μL of MHB was poured in the same wells.50 μL of solution was then removed in the second row and poured in the third row, and 50 μL of MHB was poured in that same third well.Tis procedure was repeated for the remaining wells.Tis was done in triplicate.Te results were tabulated and interpreted according to CLSI [15,16] and indicated as susceptible, moderately resistant, or resistant.To determine MBC, it was done like MIC (via serial dilution in broth media) only that the lowest dilution in which there was inhibition of growth

Petroleum ether extract
Fresh plant material Drying at room temperature (25 ±3 °C) Grind to powder (X g)

Methanol extract
Residue discarded

Ethyl acetate extract Residue
Residue 100 g

Aqueous extract
The rest (X -100) g of microorganism which was picked with a wire loop and streaked on NA.It was then incubated for 24 hours.Te dilution, in which there was no growth even after the incubation period, was taken as MBC.

Bioassay Guided Fractionation of the Most Active
Extracts.Te most bioactive extract (25 g) was fractionated on a glass column of diameter 10 cm by 40 cm height packed with 500 g silica gel (70-230 mesh) sequentially, with the aim of identifying potent compounds that might be responsible for the plant's activity.A5000 mL of the eluting mixture was obtained for every fash column chromatography that was carried out.Tin layer chromatography was extensively applied in determining the most efective solvent system to use.Requirements for fractionation using fash chromatography included analytical grade silica gel (70-230 mesh), Whatman flter papers, cork, and glass wools, fve beakers of 1000 mL each, solvents (petroleum ether, ethyl acetate, and methanol), and dry ethyl acetate extract of C. fascicularis root bark.Use of solvent systems of increasing polarity was necessary to ensure that most components of CFR2 were separated out from it.Lack of any more colour change in the solvent system dictated the change to the next solvent system.Fractionation started with petroleum ether 100% solvent system followed by 25% PE/EtOAc solvent system, then 50% PE/EtOAc solvent system, and fnally 100% EtOAc solvent system.Te fractions obtained were then concentrated in a rotor vapour machine at a temperature of 45 °C.Tis moderate temperature ensured that the volatile compounds in the crude extract did not evaporate.Some of the fractions obtained were pulled together depending on their similarity in colour for each solvent system that was used.TLC analysis was performed on these fractions, and based on the similarity in their retention factors (R f ), some were also pulled together.Seven fractions were obtained, and together with the ethyl acetate root bark extract, they were bioassayed against the four strains of bacteria using disc difusion and tube dilution methods described earlier under antibacterial screening.

Bioassay Guided Fractionation of the Most Active
Fractions.Most active fraction was further fractionated using pure forms of petroleum ether, diethyl ether, and ethyl acetate.Te subfractions obtained were bioassayed together with the bioactive fraction.A fractional concentration of 500 μg/mL was used.Gentamicin (Gtn) was the positive control at a concentration of 10 μg/mL.2.2.6.Identifcation of Bioactive Compounds.Phytochemical screening, GC-MS, and HPLC-ESI-MS methods were used for the characterization of bioactive compounds in the most active fraction.

Qualitative Phytochemical Screening.
Qualitative phytochemical screening of the most active fractions for alkaloids, favonoids, phenols, and terpenoids/terpenes was done.

Alkaloids.
Te test was performed by using the method of Harborne [17].Extract of 0.1 g was mixed with 1 mL of methanol and 1 mL of 0.01 M HCl on a hot water bath for 2 minutes and then fltered.0.5 mL of Wagner's reagent (0.5 g of iodine dissolved in 20 mL of distilled water in which 1.2 g of KI is added) was added to the fltrate.Insoluble precipitates could demonstrate the presence of alkaloids.
2.2.9.Flavonoids.Te test was performed by using the method of Harborne [17].0.1 g of powdered sample was heated with 1 mL ethyl acetate, over steam bath (40-50 °C) for 5 minutes.Ten, 0.1 amL of ammonia solution was added to fltrate.Yellow coloration could indicate the presence of favonoids.
2.2.10.Phenols.Te test was performed by using the method of Sofowora [18].To 0.1 g of extract, 5 mL of distilled water was added.A drop of ferric chloride was then added to the solution of extract.A green precipitate could demonstrate the presence of phenols.

Terpenoids/Terpenes (Salkowski Test).
Salkowski test was performed by using the method of Edeoga et al. [19].Addition of 0.5 mL of chloroform was carried out to 0.1 g of extract.Ten, 0.5 mL of concentrated sulphuric acid was carefully added to form a layer.A red-brown colouration on the interface could indicate the presence of terpenoids/terpenes.

Sample Preparation.
Fraction 2 was air-dried for 12 hrs to remove ethyl acetate solvent.0.2 g of dry fraction 2 was then dissolved in 20 mL of methanol, HPLC grade making up the sample for analysis.A portion of this sample was used for GC-MS analysis, while the remaining portion was used in LC-ESI-MS analysis.

GC-MS Analysis.
A Shimadzu QP 2010-SE GC-MS coupled with an auto sampler was used for analysis.Ultrapure helium was used as the carrier gas at a fow rate of 1 mL/min.A BPX5 nonpolar column, 30 m; 0.25 mm ID; 0.25 μm flm thickness, was used for separation.Te GC was programmed as follows: 50 °C for 1 minute; then set to increase temperature by 5 °C/min up to 150 °C in 20 minutes.Total around-time was 41 minutes.Only 1 μg of the sample was injected.Injection was conducted at 240 °C in the split mode, with the split ratio set to 10 : 1. Te interface temperature was set at 250 °C.Te electron ionization (EI) ion source was set at 200 °C.EI is a harsh ionization technology.A mass analysis was conducted in the full scan mode [20].A nonpolar column was used for separation because most volatile compounds to be analysed via GC-MS are nonpolar, and based on the general chemical principle that "likes dissolve like," then this column would separate these compounds very well [21].Confrmation of structures was performed by comparing with NIST library 14 [22] in terms of similarity index.

4
Journal of Chemistry

Data Analyses.
Te efect of the various plant extracts on the bacterial isolates was analysed via measurement of their average inhibition zones separately.ANOVA testing was carried out to show efcacy of the plant extracts against the bacterial strains.Means of the inhibition zones were separated using Tukey's honest signifcant diference (HSD) test [24].

Results and Discussion
3.1.Percentage Yield of Plant Extracts.Te weight of the plant part extracts of C. fascicularis DC., that is, CFR, CFS, and CFL, was taken and recorded, and the percentage yield was calculated using the following formula: % Yield � weight of plant extract weight of original powdered plant part × 100. (1)

Antibacterial Activity of Crude Extracts of C. fascicularis.
Since the activity of each crude extract was considered relative to the other, the crude extract that showed the largest zone of inhibition against a particular strain was considered the most active against that strain.

Minimum Inhibition Concentrations of Leaves, Stem Bark, and Root Bark of C. fascicularis against Microorganisms.
Te ethyl acetate and methanolic extracts obtained from leaves of C. fascicularis were slightly efective on inhibition of S. aureus than petroleum ether and water extracts from the leaves of C. fascicularis.However, using one-way analysis of variance, the efects were not signifcant (P � 0.621).In addition, the methanolic extract from the leaves of C. fascicularis had inhibition on S. typhi, B. subtilis, and E. coli although they were not signifcant (P > 0.05) (Table 2).Te ethyl acetate extract obtained from the stem bark of C. fascicularis (CFS2) was signifcantly efective on the inhibition of S. aureus, S. typhi, E. coli, and B. subtilis in comparison to methanol, petroleum ether, and water extracts obtained from the stem bark of the same plant (F � 3.08, P � 0.031) compared to (F � 3.20, P � 0.027, F � 2.96, P � 0.037 and F � 2.82, P � 0.043, respectively).Te methanolic extracts obtained from the stem bark of C. fascicularis (CFS3) had higher inhibition (F � 2.82, P � 0.043) against B. subtilis.
In addition, more signifcant results were recorded from the ethyl acetate extract obtained from the root bark of C. fascicularis (CFR2) against S. aureus (average diameter of inhibition zone � 9.2 ± 0.8 mm, P � 0.0001) compared to S. typhi, E. coli, and B. subtilis (Table 2).
Moreover, CFR2 showed a more signifcant inhibition against S. typhi, B. subtilis, and E. coli (average diameter of inhibition zones � 7.9, 7.3, and 6.3 mm and P value � 0.0001,   8 Journal of Chemistry 0.0001, and 0.045, respectively) compared to CFR1 and CFR4 (average diameter of inhibition zones � 6.0 mm and P value >0.05), respectively.Te methanol extract obtained from the root bark of C. fascicularis (CFR3) was more efective against B. subtilis (average diameter of inhibition zone � 6.3 mm and P � 0.0001).
Te CFR2 was signifcantly efective on inhibition of selected bacterial strains (P � 0.0001 for S. aureus, B. subtilis, and S. typhi and P � 0.045 for E. coli) and (average diameter of inhibition zone � 9.2, 7.3, 7.9, and 6.3 mm for S. aureus, B. subtilis, S. typhi, and E. coli, respectively (Table 2).Gentamicin was used as the positive control and had high inhibition on bacteria at a low concentration of 10 μg/mL.Negative control was the solvent in which the plant extracts were dissolved, which showed no activity.
Table 3 shows the minimum bactericidal concentration (MBC) for the C. fascicularis solvent extracts.
Te MBC of CFR2 was recorded at a concentration of 500 μg/mL against B. subtilis, 250 μg/mL against S. typhi, and 125 μg/mL against S. aureus.However, these MBC values were higher compared to that of the commercial antibiotic, gentamicin (10 μg/mL) against the selected bacterial strains.

Antibacterial Activity of Fractions from Ethyl Acetate
Extract of Capparis fascicularis Root Bark.Te following plates (Figures 2 and 3) show the antibacterial activities of fraction 2 and subfractions of fraction 2, respectively.
Te average diameters of zones of inhibition for the fractions of CFR2 and itself on bacteria at diferent concentrations, F-values, and P values are recorded in Table 4.
Fractions with concentrations of 500 μg/mL and 250 μg/ mL had the highest inhibition zones on the selected bacteria.Te most inhibitive concentration was noted at 500 μg/mL, which was signifcantly higher than the subsequent concentrations (P value <0.05).Tis was with the exception of gentamicin which was the most inhibitive against the selected bacteria at a concentration of 10 μg/mL.Moreover, signifcant MIC values against S. aureus, S. typhi, E. coli, and B. subtilis were at 62.5, 125, 125, and 250 μg/mL, respectively, with P value <0.05 (Table 5).
Te minimum inhibitory concentrations (MIC) of 500 μg/mL for F 2 were signifcantly higher against S. aureus (average diameter of inhibition zone � 16.0 mm and P value � 0.0001).However, F EtOAc at 500 μg/mL was signifcantly lower on B. subtilis bacteria (average diameter of inhibition zone � 7.33 mm and P � 0.0001) (Table 6).

Most Bioactive Fractions.
Considering the MIC values, the signifcantly most bioactive species was the positive control, Gtn (mean � 18.500 mm) at 10 μg/ml against selected bacteria.Tis was followed by F 2 (mean � 13.250 mm against selected bacteria) at 500 μg/ml, and so, it was signifcantly the most bioactive fraction compared to the four subfractions of itself (F � 66.79, P � 0.0001) (Table 7).
To establish the interactive efect of the subfractions and the bacteria, a two-way analysis of variance (two-way ANOVA) was carried out on the zone of inhibition.Interaction was found to be signifcant (F � 246.38,P � 0.0001).
From Tables 6 and 7, the subfractions had a lower average diameter of inhibition on bacteria than fraction 2 after being tested at a higher concentration of 500 μg/mL.Tis suggested that the constituents of fraction 2 worked in synergy.Fraction 2 was a pull of fractions from the 50% PE/ EtOAC solvent system.It was analysed using spectroscopic methods to identify the compounds responsible for its high bioactivity.Separate work on these is being carried out in our research team.Table 8 shows the results for the qualitative phytochemical analysis of F 2 .

Journal of Chemistry
From the results in Table 8, yellow colouration was observed which demonstrated a positive test for favonoids and a green precipitate was observed that indicated presence of phenols, while a red-brown colouration on the interface was observed which indicated the presence of terpenoids/ terpenes.Tese results are based on the procedure given under methodology.
(1) GC-MS Analysis.A GC-MS scan was carried out of fraction 2, and it was found to contain antibacterial compounds.Compounds which had major peaks were identifed by use of NIST 14 library [22] and had the highest similarity index.Te GC-MS chromatogram is shown in Figure 4, while the compounds are indicated in Table 9.However, only two compounds were bioactive.
Figures ( 5)-( 10) are the six compounds identifed by GC-MS analysis as shown in Table 9.It is important to note that structure 1 (di-ethylphtalate), having the highest similarity index, was not a metabolite but a contaminant.It is manufactured by reacting phthalic anhydride with alcohol [25].Accumulation of phthalates may occur in a variety of herbal medicines, especially those which are growing up in water and rivers due to the exposure of plants' roots to the polluted waste water.In report by Azadeh Manayi [26] phthalate esters were found in the essential oil of roots of Achillea tenuifolia of family Compositae, a similar case with C. fascicularis of family Capparaceae that has been reported to grow along rivers [9,27].Tis plant was sampled along a seasonal river in Githabai ward characterised with human activities such as washing of clothes along river banks which exposes it to plastic wastes from emptied detergent containers and sachets.Te compound, which has been used as a plasticizer in chemical and pharmaceutical industries, is able to be simply released into the water and soil and accumulate in the plants even in the medicinal species that are growing wildly in mountainous areas surrounded by lots of municipal solid wastes, disposed plastics, and water bottles.
Structures of Compounds of the Major Peaks Identifed by GC-MS Analysis.
Table 10 gives the names, retention times, molecular weights, and positive ion mode fragments of these compounds.
Figures (11) and ( 12) are the compounds identifed by positive HPLC-ESI-MS analysis as shown in Table 10.
Tanshinone II A (Figure 11) compound was identifed to have a protonated molecular ion [M + H] + with a molecular weight of 295 and 2 protonated fragment ions [F + H] + with molecular weights of 277 and 249.Tis was as reported in the literature [28].
Table 11 gives the retention time, molecular weight, and negative ion mode fragments of this compound.Compound (Figure 15) was also identifed together with Tanshinone II A (Figure 13) and Cryptotashinone (Figure 14) in Salvia miltiorrhiza Bunge (Lamiaceae) and has previously been shown to possess signifcant antibacterial activity.It has also shown great potential applications in the pharmaceutical and medicinal industry [26].

Conclusion
In conclusion, the ethyl acetate extract from the root bark of C. fascicularis has signifcant activity against both Gram-positive and Gram-negative bacteria.It has MIC values of 31.25, 62.5, 62.5, and 500 μg/mL against S. aureus, B. subtilis, S. typhi, and E. coli, respectively.It contains phytochemical substances such as phenols, terpenoids, and favonoids.Our future study will be based on the isolation and bioassay of the bioactive compounds in the ethyl acetate extract from the root bark of C. fascicularis.

Figure 1 :
Figure 1: Organic and aqueous extraction procedure for C. fascicularis plant parts.

Figure 4 :
Figure 4: A chromatogram of major peaks identifed in the GC-MS analysis.

Table 1 :
Percentage yields of plant extracts.

Table 2 :
Average diameter of zones of inhibition by C. fascicularis plant parts (mm) (mean ± SE).

Table 4 :
Average diameters of zones of inhibition for fractions of CFR2 against the bacterial strains.FractionsS.aureusS. typhi B. subtilis E. coli Average diameters of inhibition zones (mm) (mean ± SE)SE: standard error values of the mean in the same column referred by alike alphabetic characters are not signifcantly diferent when P ≤ 0.05.Tukey's HSD is used to separate the mean.

Table 5 :
Minimum inhibitory concentrations of fractions of CFR2 against bacteria.
SE: standard error values of the mean in the same column referred by alike alphabetic characters are not signifcantly diferent when P ≤ 0.05.Tukey's HSD is used to separate the mean.

Table 6 :
Average diameters of inhibition zones for the F PE , F DE , F EtOAc , F R subfractions, and F 2 and Gtn on bacteria.PE (Petroleum ether fraction), F DE (diethyl ether fraction), F EtOAC (ethyl acetate fraction), F R (residue fraction), F 2 (fraction (2), Gtn (gentamicin).Values of the mean in the same column referred by alike alphabetic characters are not signifcantly diferent when P ≤ 0.05.Tukey's HSD is used to separate the mean.Note.Where the values had no variation with F and P values, they were marked as (−). F
Values of the mean in the same column referred by alike alphabetic characters are not signifcantly diferent when P ≤ 0.05.Tukey's HSD is used to separate the mean.

Table 9 :
Compounds of the major peaks identifed in the GC-MS analysis.

Table 10 :
Compound identifcation results for fraction 2 by positive mode HPLC-ESI-MS.

Table 11 :
Compound identifcation results of fraction 2 by negative mode HPLC-ESI-MS.