2. Materials and Methods
2.1. Data Collection
The study material consisted of three types of cheese, produced using traditional methods in small farms located in southern and eastern Poland. In terms of the ripening time, 2 types of cheeses were classified as short-ripened (ripening time of 7–14 days) and one cheese type was classified as long-ripened (ripening time of 3 months). Among the short-ripened cheeses, one group consisted of smoked cheeses and the other group of natural cheeses (commonly referred to as “oscypek,” although in legal terms the name is reserved only for cheeses produced from sheep’s milk or from cow’s and sheep’s milk mixed in adequate proportions). Both short-ripened cheeses were produced in one manufacture, and long-ripened cheeses were produced in another manufacture. Twelve samples of each type of cheese were collected in the same season (winter). Cheeses (n=36) were purchased in the retail stores of the manufacturers. The samples were delivered to the microbiological laboratory within one hour of collection and maintained at a temperature between 0°C and +4°C during transport.
In the direct conversation with the manufacturers of the examined cheeses, the following information was obtained: all three cheese types are listed as traditional products. The material for the production of short-ripened cheeses was pasteurized cow’s milk obtained from dairies, used for the production within the period of 48 h from the purchase. The material for the long-ripened cheeses was raw cow’s milk from the farm of the cheese manufacturer, used for the production within 2 h after collection (mechanical milking). Commercial dairy cultures were used in the production of the three examined cheese types, and production and ripening room temperatures were 18–20°C and 14–20°C, respectively.
2.2. Microbiological Analysis
Microbiological counts of aerobic bacteria, psychrotrophic bacteria, lactic acid bacteria, coliform bacteria, E. coli, Enterobacteriaceae, Enterococcus spp., Staphylococcus spp., and yeasts were performed by placing a 10 g of a sample and 90 mL of saline peptone water in a stomacher bag and subsequently homogenizing it in a stomacher for 2 min at normal speed (230 rpm). From this dilution, other decimal dilutions were prepared (PN-EN ISO 6887) and plated on the appropriate media according to the International Organization for Standardization standards (ISO) and Polish Standard (PN-ISO 4833-2, PN-ISO 17410, PN-ISO 15214, PN-ISO 4832, PN-ISO 16649-2, PN-ISO 21528-2, PN-A-82055-7, PN-EN ISO 6888-1, and PN-ISO 21527-1). To determine the count of aerobic and psychrotrophic bacteria, plates with nutrient agar medium were used, incubated at 30°C for 72 h and at 4°C for 10 days, respectively: for lactic acid bacteria, plates with MRS medium, incubated at 30°C for 72 h; for coliform bacteria, plates with VRBL medium, incubated at 37°C for 24 h; for E. coli, plates with TBX medium, incubated at 44°C for 18 h; for Enterobacteriaceae, plates with VRBG medium, incubated at 37°C for 24 h; for Enterococcus spp., plates with Slanetz–Bartley medium, incubated at 37°C for 48 h; for Staphylococcus spp., plates with Baird Parker medium, incubated at 37°C for 48 h; and for yeasts, plates with Sabouraud dextrose medium with chloramphenicol, incubated at 25°C for 5 days. For the identification of Staphylococcus, Enterococcus, and E. coli, nonreagent Erba Lachema tests (Brno, Czech Republic) were used: Staphytest 24, Streptotest 24, and Enterotest 24 new, as well as Multiscan EX test reader. The number of microorganisms is provided in log cfu/g.
In order to determine the presence of Salmonella spp., an examination was performed according to PN-EN ISO 6785. Twenty-five grams of a sample in 225 mL of buffered peptone water was homogenized in a laboratory blender (Stomacher 400) and incubated at 37°C for 18 h. The media used subsequently were Rappaport-Vassiliadis incubated at 41.5°C for 24 h, and then, simultaneously, XLD and BGA were incubated at 37°C for 24 h (in the case of the absence of typical colonies on XLD and BGA media, the test was discontinued according to the procedure).
In order to determine the presence of Listeria spp., an examination was performed according to PN-EN ISO 11290-2. Twenty-five grams of a sample in 225 mL half Fraser broth was homogenized in a laboratory blender (Stomacher 400) and incubated at 30°C for 24 h. Then, the Fraser broth was used, incubated at 37°C for 48 h, and, subsequently, Ottaviani and Agosti medium (ALOA) along with Oxford medium was used, incubated at 37°C for 24–48 h. Confirmatory tests were performed (Gram staining, mobility capacity, sugar fermentation, and hemolysis test). The presence of Listeria monocytogenes was confirmed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (Bruker, Germany).
All microbial media used in the study were purchased from Biocorp (Poland).
2.3. Statistical Analysis
All the data are presented as means and ±SD. The statistical analysis of the data was performed using the Statistica software (StatSoft, Poland, version 13.1) and were determined by the one-way ANOVA and Tukey’s multiple comparison post hoc test; P<0.05 was considered as statistically significant. Correlations were determined by Pearson’s correlation coefficient (r).
4. Discussion
The total bacterial count was similar in smoked and natural short-ripened cheeses (log 7.97 cfu/g) and lower by approximately 2 log than in long-ripened cheeses (log 10.19 cfu/g). The total bacterial count of the examined long-ripened cheeses was higher than that in long-ripened cheeses produced using traditional methods from raw cow’s milk in Spain and Italy, for which the total aerobic count, depending on the ripening stage, was from 8 to 9.06 log cfu/g [6, 15]. The total bacteria count in short-ripened cheeses produced regionally using traditional methods, that is, smoked and natural oscypek cheeses from sheep’s, cow’s, and mixed milk and golka cheeses from mixed (sheep’s and cow’s) milk was at the level of 108–1010 cfu/g (see [39], cited in [7], and [16]). In the presented study, the values of total microbiological count of both short-ripened cheese types (smoked and natural) were similar to the lower levels of the range (approximately 108 cfu/g). Smoked and natural oscypek cheeses produced using the traditional method from sheep’s milk differed in the total bacteria count, which was higher in natural cheeses (resp.: 105–108 and 107–109 cfu/g) [7]. In the presented study, no significant differences in the level of the total bacteria count were determined between smoked and natural cheeses, and their total bacteria count remained in the ranges of values determined by Berthold-Pluta et al. [7] Three types of ripened goat cheeses had similar total bacteria count of about 3.2 × 109 cfu/g, which was not affected by the milk type (raw, pasteurized, and high-pressure-treated). According to the authors of this study, the starter bacteria inoculated into the cheese milk affected the total bacterial count [17].
The count of psychrotrophic bacteria was higher in long-ripened cheeses (log 9.40 cfu/g) than in short-ripened natural and smoked cheeses (log 7.28 and log 6.92 cfu/g, resp.). The number of these bacteria in the product depends, for example, on their level in the raw milk and its storage temperature. Psychrotrophic bacteria are naturally present in milk, where they can reach counts of up to 105 cfu/mL [18]. Raw milk is usually refrigerated, especially when it is not subjected to processing directly at the dairy farm. The increase of the population of psychrotrophic bacteria in the milk can be inhibited by storing it at 4°C and by thermization. The psychrotrophic bacteria count was reduced approximately to log 2 cfu/ml and was stable during storage at 4°C for about 7 days [19]. The use of higher temperatures, that is, pasteurization or pressure treatment, reduced the number of psychrotrophic bacteria in raw milk from 7 × 105 cfu/mL to <10 and 10 cfu/mL, respectively [17]. In the current study, lower psychrotrophic bacteria count in the short-ripened cheeses in comparison to the long-ripened cheeses may stem from heat-treated (pasteurized) milk used in the production of short-ripened cheeses. The total psychrotrophic count in traditional goat cheeses ranged from log 8.6 (in cheeses produced from milk which was cooled after milking) to log 9.1 cfu/g (in cheeses produced from milk which was not cooled after milking) [9]. The examination of the refrigeration chain was not the objective of the present study; therefore, it was not possible to determine the impact of temperature on the count of these bacteria in the final products.
Two fundamental groups of microbiota are found in cheeses: the starter microbiota, which are added at various stages of cheese production, and nonstarter (native) microbiota, which can originate from, for instance, milk, equipment, or the production environment. The main group of cheese microbiota consists of lactic acid bacteria (LAB), which are included in both starter and nonstarter microbiota [20]. LAB are the dominant microbial group; they usually reach levels above 9 log cfu/g within the first day of cheesemaking and remain dominant until the end of the process of ripening, although there occur certain variations in the species balance in the course of this process [3]. The lactic acid bacteria count in long-ripened cheeses was significantly higher (log 10.38 cfu/g) than that in short-ripened cheeses (log 8.30–8.45 cfu/g). This ensued from a longer ripening time. Lactic acid bacteria were the dominant bacteria group in all the examined cheeses and other traditional short-and long-ripened cheeses from Northern Morocco and Italy [9, 15]. In long-ripened cheeses produced using traditional methods in Italy and Argentina, the lactic acid bacteria count was, depending on the ripening stage, log 7-log 9.07 cfu/g for cow’s milk cheeses [15, 21] and log 9.8 cfu/g for short-ripened cheeses of the golka type (mixed sheep’s and cow’s milk) from Poland [16].
Microorganisms such as coliform bacteria, E. coli, Enterobacteriaceae, and Enterococcus faecalis determined the production hygiene of the examined cheeses. The counts of coliform bacteria under the conditions of good production practice should not exceed 103 cfu/g (see [40], cited in [22]). Contamination with coliform bacteria in each of the examined cheeses exceeded this level (log 5.35–8.36 cfu/g). The lowest count of these microorganisms was determined in short-ripened smoked cheeses, significantly higher in natural cheeses, and the highest in long-ripened cheeses. The results obtained by other authors demonstrated high divergence in the level of coliform contamination in different types of cheese produced using traditional methods from cow’s, goat’s, sheep’s, or mixed milk, which ranged from under 1 to 7.89 log cfu/g [6, 9, 15, 23–25]. The level of coliform bacteria in ripening cheeses changed during the ripening time. In a traditional mountain cheese from Italy, the highest coliform count (log 4.7 cfu/g) was observed 24 h after production, which was probably due to the fact that the pH reduction by the autochthonous starters took 24 h. At the end of ripening, very low concentrations of coliform bacteria were detected [23]. Similarly, in traditional Greek cheeses, the number of coliform bacteria was reduced during ripening, and usually they were not detected in mature cheeses [25]. Coliform bacteria count was reduced even during curd draining, which was probably due to unfavorable conditions created by the increasing population of LAB and the decreasing pH [25]. In contrast to the results of the abovementioned study, an increase of coliform bacteria until the end of ripening was determined for traditional, starter-free cheese made from cow’s milk [6].
The number of E. coli in the three examined cheese types ranged from 3.30 to 4.78 log cfu/g and remained within the ranges of values determined by other authors. The level of E. coli in long-ripened cheeses produced using traditional methods in Italy, Brazil, and Argentina was from under 1 to 5 log cfu/g or log MPN (most probable number)/g [8, 21, 24, 26] and in short-ripened cheeses from Poland, that is, smoked and natural oscypek type cheeses, from 0 to over 102 MPN/g. Oscypek smoking did not result in a reduction of the number of E. coli [7], but the ripening period at room temperature had a significant impact on the reduction of their count in cheeses [24]. The higher coliform bacteria and E. coli counts at the beginning of ripening were connected with raw milk which was collected manually and coagulated at low temperature. The cheese ripening process caused a decrease in the bacteria count or their elimination as a result of increased lactic microflora, lowered pH, and increased dry matter and chloride content of the cheese [8].
Three types of cheeses in the present study differed significantly in terms of the counts of Enterobacteriaceae, which ranged from log 5.53 to 8.28 cfu/g. Numbers of microorganisms in this group for other ripened cheeses remained within broad ranges and amounted from 2.49 to 7.25 log cfu/g for cheeses produced in Greece and the Island of Tenerife from sheep’s and goat’s milk [25, 27], whereas from 1.0 × 10 to 1.24 × 108 cfu/g for those produced in Spain from cow’s milk [10]. Environmental contamination and handling during cheesemaking may be the reason behind the presence of Enterobacteriaceae in the cheeses [17]. The level of Enterobacteriaceae depended on the time of cheese ripening; as the ripening time was extended, the levels of these bacteria decreased (about log 0.6–1 cfu/g) [25]. Alegría et al. [30] reported that the smoking process increases the quality and safety of the traditional oscypek cheese; the number of Enterobacteriaceae in smoked cheeses was lower (about log 0.4–2 cfu/g) than that in cheeses before smoking. This is corroborated by the present study, as the count of Enterobacteriaceae was significantly lower (about log 1 cfu/g) in smoked cheeses in comparison to natural cheeses.
Enterococci occur as nonstarter lactic acid bacteria in various cheeses, and particularly in artisan cheeses. They can be found in cheeses which are made from both raw and pasteurized milk. Their levels depend on various factors, including the extent of milk contamination, the type of cheese, the starter used, and the technology applied, such as their survival and growth under the specific conditions of cheese manufacture and ripening [28]. The count of Enterococcus spp. was similar in short-ripened natural and smoked cheeses (log 6.15 and log 6.44 cfu/g, resp.) and lower by about 2 log than in long-ripened cheeses (log 8.22 cfu/g). Levels of enterococci in a fully ripened cheese may range from 105 to 107 cfu/g [28]. In comparison to these levels, the number of these microorganisms in short-ripened cheeses remained within this range, while it was higher in long-ripened cheeses. The presence of high numbers of Enterococcus spp. is typical for cheeses obtained from raw milk [6]. On the contrary, the presence of enterococci in pasteurized milk can be the result of contamination during cheesemaking [17]. This explains the differences in the amounts of these bacteria in the examined cheeses. Enterococcus spp. count in traditional cheeses made of milk which was not heat-treated was, depending on the ripening stage, from under 1 to 6.97 log cfu/g [6, 25–27, 31]. Compared to these data, the number of Enterococcus spp. in the studied short-ripened cheeses was similar to the upper level of the range, and in long-ripened cheeses it was considerably higher. The presence of a high number of Enterococcus spp. could result from poor hygienic practices during the manufacturing process and the resistance of Enterococcus spp. to adverse conditions (high temperature, high salt concentration, and high acid levels) [27]. E. faecalis was determined in all samples of cheese examined in the present study, which presented a risk to potential consumers. On the other hand, Enterococcus spp. is considered as emerging pathogen for humans. Around 80–90% of human enterococcal infections are caused by Enterococcus faecalis, while a majority of the remainder is accounted for by Enterococcus faecium (see [41], cited in [29]).
In the cheese ripening process, besides the role of the starter lactic acid bacteria, an important contribution is made by the secondary microbiota, which includes, among others, yeasts [32]. Yeasts can play a significant role in sensory and functional properties of cheese ripening, based on the interactions between yeasts and starter cultures, proteolytic and lipolytic activities, aroma compound formation, and other metabolic activities [33]. However, yeasts can also be responsible for some of the major defects in cheesemaking (see [42], cited in [34]). The growth of yeast is intensive on the cheese surface, and yeast counts range from around log 6 to log 8 cfu/cm2 within 2–7 days; then, they remain fairly constant until the end of ripening, with variations in species balance [3]. In the examined short-ripened natural and smoked cheeses, the number of yeasts was comparable (log 5.23 and log 4.99 cfu/g resp.) and significantly lower than that in long-ripened cheeses (log 7.74 cfu/g). The differences between the amounts of yeasts in the examined short- and long-ripened cheeses could be the consequence of different ripening times. The number of yeasts in the examined short-ripened cheeses remained in the range determined for yeasts and moulds in traditional cheeses with a similar ripening time, that is, oscypek cheeses produced from sheep’s milk (log 5.11–6.56 cfu/g) [30]. On the contrary, the number of yeasts in the examined long-ripened cheeses was higher than that in other traditional long-ripened cheeses produced from cow’s milk (log 2.18–7.21 cfu/g), goat’s milk (log 3.89–4.66 cfu/g), and sheep’s milk (log 4.43–6.37 cfu/g) [21, 25–27].
Staphylococcus spp. were found in all three examined cheese types and they were at a similar level (log 6.26–6.46 cfu/g). The number of these microorganisms in traditional ripened cheeses produced from raw sheep’s or cow’s milk was from log 3.54 to log 6.50 cfu/g, depending on the ripening stage [6, 25]. In the current study, the presence of Staphylococcus aureus was confirmed in three samples of long-ripened cheeses. S. aureus was recorded at the level of about log 1–6.41 cfu/g in ripened cheeses made from goat’s and cow’s milk [21, 24, 35]. These microorganisms could be inhibited by highly competitive lactic acid bacteria which can survive ripening conditions. Factors such as the type of the starter used, reduction of water activity, increased NaCl concentration, ripening time, and higher ripening temperature could affect the rate of reduction in the pathogen counts [24, 35]. S. aureus was not detected in traditional ripening cheeses produced in Italy and Poland [16, 23]. S. aureus at levels over log 5 cfu/g can produce enterotoxins in an amount which can be dangerous for human health [36].
In the examined three types of cheeses, no presence of Salmonella microorganisms was detected. Similarly, these microorganisms were not revealed in other short- and long-ripened traditional cheeses produced in Poland, Brazil, Northern Morocco, and Spain [9, 10, 16, 24]. The presence of Salmonella species was confirmed in 2.4% of samples of Tulum cheeses from Turkey, which could be related to the use of raw milk, unhygienic production process, and storage conditions [37]. According to these authors, it is essential that high safety standards are ensured, such as raw milk quality, effective pasteurization process, hygienic production and storage conditions, and proper cleaning and sanitation processes in production places [37].
In 3 samples of the examined short-ripened natural cheeses, Listeria was revealed, including Listeria monocytogenes in 1 sample (2.8%). The presence of this pathogen in this type of cheese, which is produced from pasteurized milk, can be explained by the fact that Listeria spp. can be isolated from milk heated at 60–67.5°C, and L. monocytogenes survived in high-temperature short-time pasteurization (see [43] and [44], cited in [2]). The source of the contamination of cheeses with L. monocytogenes may be both raw milk used in cheese production and cheese-processing environment, that is, the conditions of manufacture, ripening, and storage [38]. L. mononocytogenes was also found in traditional homemade cheeses from Northern Morocco and Western Turkey in 4 (14%) and 13 (9.2%) samples, respectively [9, 11]. The results obtained by Fox et al. [1] demonstrated that the production environment may be the source of L. monocytogenes contamination of cheeses (75% of samples from cheesemaking facilities were found to have been contaminated with L. monocytogenes in the processing environment).
Listeria spp. was not found in traditional long-ripened cheeses produced in Italy and Brazil or in short-ripened cheeses produced in Poland [16, 23, 24]. The lack of pathogens can be related to the existence of lactic acid bacteria strains able to inhibit cheese pathogens, including L. monocytogenes [3]. Ceugniez et al. [34] reported that yeast (Kluyveromyces lactis and Kluyveromyces marxianus) could inhibit the growth of pathogens (e.g., L. monocytogenes) in a traditional handmade cheese.