Use of Endemic Date Palm (Phoenix dactylifera L.) Seeds as an Insoluble Dietary Fiber: Effect on Turkey Meat Quality

Ecole Nationale d’Ingénieurs de Sfax, Laboratoire Analyse, Valorisation et Sécurité des Aliments, Université de Sfax, Route de Soukra, Sfax 3038, Tunisia Institut Supérieur de Biologie Appliquée de Médenine, Université de Gabès, Sfax, Tunisia Laboratoire de Génie Enzymatique et de Microbiologie, Equipe de Biotechnologie des Algues, Ecole Nationale d’Ingénieurs de Sfax, Université de Sfax, Sfax 3038, Tunisia Laboratoire de Biotechnologie Microbienne, Enzymatique et de Biomolécules (LBMEB), Centre de Biotechnologie de Sfax, P.B. 1171, Sfax 3018, Tunisia


Introduction
Today, worldwide dates production and consumption continued to increase. According to FAOSTAT, the world production has reached an average of 8, 526, 218 tons in 2018 [1]. In Tunisia, date production was about 260, 000 tons/year [2] with dominance of Deglet Nour cultivar which present about 60% of the total production and having a very substantial sensory quality and a high commercial value [3]. A major byproduct obtained from the date processing industries is the seeds representing from 10 to 15% of the fruit mass [3][4][5]. erefore, date seeds pose a problem for the industry and the environment, while they contain many valuable substances such as carbohydrates, oil, dietary fiber, protein, bioactive polyphenols, and natural antioxidants. e chemical composition of date seeds showed a high amount of fiber (75-80%), fat (10-13%), proteins (5-6%), and ash (0.9-1.8%) [6][7][8][9][10][11]. At present, date seeds were discarded, used as feed for livestock, or rarely used in food applications such as coffee, bread addition, and chocolate enrichment [3,9,12,13]. Date seeds would be regarded as an excellent source of food ingredients and could also be used as an important source of dietary fiber and protein [3,6,9,11,14,15].
Nowadays, consumers prefer products with new and safe natural ingredients. Additionally, their demand for food products with higher nutritional values and health benefits was increased. e development of natural and functional ingredients such as dietary fiber represents enormous economic potential. In this context, fibers were used by the food industry due to their medicinal virtues and technofunctional properties [16]. Recommended at a range from 25 to 30 g for the daily intake [3], fibers were associated with health benefits. In fact, with adequate intake of dietary fibers, fibers reduced the risk of constipation, obesity, diabetics, heart complications, colon and rectal cancer, gallstone, piles, hernia, atherosclerosis, and inflammation, among others [5,[17][18][19]. Consecutively, these health benefits attracted the consumer to fiber-rich foods. Dietary fibers can be integrated in food products (meat, dairy, jam, soup, and bakery products) to enhance textural properties, avoid syneresis, and stabilize high-fat food and emulsions [16,[20][21][22][23].
On the other hand, the meat products' industries must innovate and follow the technological changes to develop new formulations that are satisfying the consumer. erefore, meat products have a negative image due to hidden fat. Generally, animal fat provides high amounts of saturated fatty acids and cholesterol and that is associated with a wide range of health consequences [24]. e current trend in meat industries and consumer preference towards natural alternatives opens the possibility of applying date seeds in meat products. Hence, the addition of functional components from date seeds in the formulation of meat products will be widely recognized by enhancing quality and making health-promoting meat products with high nutritive value. us, the objective of this study is to propose the development of new meat product: turkey burgers formulated with insoluble fiber extracted from Deglet Nour date seeds. Here, we (i) characterize the physicochemical and technofunctional of the date seeds powder (DSP) and its insoluble extract (IFC) and (ii) study the technofunctional, textural, and sensory qualities of turkey burgers formulated with IFC.

Source of Materials.
e Deglet Nour date seeds were collected in the beginning of the 2017 harvest season from green fruits company, Kebili region, Tunisia (N: 33°, E: 9°). e seeds of Deglet Nour cultivar were directly isolated from date fruit having the same origin, collected at the "Tamr stage" (full ripeness) and kept at 10°C until analysis. e various ingredients used for the formulation of turkey meat burgers were obtained from the Tunisian meat company (CHAHIA, Sfax, Tunisia).

Deglet Nour Date Seeds Powder (DSP) Preparation.
e seeds were drenched in distilled water, washed to get rid of any date flesh residues, and then air-dried. en, they were further dried at about 50°C during 48 h. Date seeds were ground in a laboratory heavy-duty grinder (Tekmar A10 S2, IKA, Germany). Finally, seeds were screened to obtain a fine powder with particle size <1.5 mm and stored at 4°C in dark until used for analyses ( Figure 1).

Extraction Procedure of the Insoluble Fraction from Deglet
Nour Date Seeds (IFC). Figure 2 shows the diagram to obtain the insoluble fibers concentrate from the powder of Deglet Nour date seeds according to Bouaziz et al.'s [3] method with some modifications. IFC was used as a functional ingredient in the formulation of minced turkey breast meat burgers.

Physicochemical Characterization of IFC.
e yield of IFC extraction was the amount of extract obtained from a given amount of seeds compared to a given amount of DSP. It is expressed as a percentage: e pH was measured using a pH-meter (Metrohm, 827 pH-meter) equipped with a glass probe for penetration. Water activity (Aw) was performed using Novasina ermoconstanter SPRINT equipment (TH-500, Switzerland) at 25°C.
Minerals were determined using the American Association of Cereal Chemists methods. e mineral elements, sodium, potassium, magnesium, calcium, phosphor, iron, and zinc, were determined by atomic absorption spectrophotometry [26].
Carbohydrate content was estimated by the difference of mean values, i.e., 100 − (sum of percentages of moisture, ash, protein, and lipids) [6].
Total phenolic content (TPC) was estimated using the Folin-Ciocalteu colorimetric method with a little modification as described by Kchaou et al. [27]. e extraction of phenolic compounds from DSP was carried out using absolute ethanol. Two grams of sample was mixed for 2 h with the solvent at room temperature and with continuous   e mixture was centrifuged at 5000 × g for 15 min, and the supernatant was decanted. e pellet was extracted. Supernatant was used for the determination of TPC. e percentage of TPC was expressed in gallic acid equivalent (GAE) in mg/g of sample. Gallic acid was used to obtain a standard curve with concentrations varying from 0 to 50 mg/l.  [28]. e SWC was indicated as milliliter of swollen sample per gram of initial dry matter.

Determination of Antioxidant Activity by the Anti-Free
Radical Method (DPPH). Free radical scavenging activity of DSP was evaluated using the DPPH assay according to the method of Abbes et al. [29] with minor modifications. DPPH (2,2-diphenyl-2-picrylhydrasyl) was a stable free radical of purple color. In the presence of antiradical compounds, this radical is reduced by changing color (yellow). is method is based on measuring the ability of antioxidants to trap the radical (DPPH). e latter is reduced to the form of hydrazine (nonradical) by accepting a hydrogen atom. e change of absorbency can be followed spectrophotometrically. Four hundred mg of DSP was added in 20 ml of ethanol (70%), stirred for 1 h, and centrifuged for 20 min at 4,500 rpm to recover the supernatant. Serial dilutions were prepared from the supernatant. 500 µl of each dilution prepared was mixed with 375 µl of absolute ethanol and 125 µl of a solution of DPPH (0.02% in ethanol). After 1-hour incubation in the dark, absorbance was measured at 517 nm using a spectrophotometer (Shimadzu, UVmini-1240, Japan) against a blank. e blank was constituted by the DSP extract at the same concentration, containing all reagents except DPPH. Distilled water was used as a control instead of DSP. e radical scavenging activity was calculated as follows: % inhibition � (Abs control − Abs sample) Abs control × 100, Abs: absorbance. (4) e antioxidant results were expressed as mmol equivalents of Trolox per 100 gram of sample (dry weight).

Determination of Antioxidant Activity by Free Radical
Scavenging: ABTS Test. e antioxidant capacity was estimated in terms of radical scavenging activity following the procedure described by Savago et al. [30]. Briefly, ABTS [2,2azinobis(3-ethilenzotiazolin)-6-sulfonate] radical cation (ABTS + ) was produced by reacting 7 mmol/L ABTS stock solution with 2.45 mmol/L potassium persulphate in the dark at room temperature for 12-16 h before use. e ABTS + solution was diluted with methanol to an absorbance of 0.70 ± 0.02 at 730 nm. ABTS + solution (3.9 mL) and phenolic extract (0.1 mL) were mixed, and the absorbance was read every 20 s using a spectrophotometer (Shimadzu, UVmini-1240, Japan). e reaction was monitored during 6 min. Inhibition of absorbance vs. time was plotted and the area below the curve (0-6 min) was calculated. e antioxidant results were expressed as mmol equivalents of Trolox per 100 gram of sample (dry weight).

Evaluation of Antibacterial Properties: Diffusion Technique on Agar.
e well method was used to assess antibacterial activity. is method has been described by Ilanko et al. [31]. e activity of the extract from DSP against the six microorganisms used is based on the technique of diffusion in solid medium. Wells 6 mm in diameters are dug using a sterile Pasteur pipette and are filled with 50 μl (5 mg/ml) of the extract to be tested. A preculture of Staphylococcus aureus ATCC 6538, Bacillus cereus ATCC 14579, and Listeria monocytogenes ATCCC 19117 (Gram +) and Salmonella typhimurium ATCC 14028, Escherichia coli ATCC 8739, and Pseudomonas aeruginosa ATCC 49129 (Gram −) was launched 12 to 24 h in Luria Bertani (LB) medium, respectively, at 30 and 37°C and with stirring (200 rpm) before the test in order to seed the agar medium with fairly young cells. e bacterial film is obtained following appropriate dilutions of these mother precultures, in order to obtain inocula at 10 6 -10 7 CFU/ml for the bacteria which are spread uniformly over the entire surface of the agar, in Petri dishes. Petri dishes containing 20 ml of LB agar medium were inoculated with 100 μl of a bacterial suspension at 10 6 -10 7 CFU/ml in the exponential growth phase. en, wells were dug using a sterile Pasteur pipette. DMSO was used as a negative control. e Petri dishes thus prepared were placed at 4°C for 4 h to allow the diffusion of the active substance present in the well. Finally, the dishes were incubated in an oven at 30°C for Listeria monocytogenes ATCC 19117, Salmonella typhimurium ATCC 14028, and Pseudomonas aeruginosa ATCC 49129 and at 37°C for Escherichia coli ATCC 8739, Bacillus cereus ATCC 14579 37°C, and Staphylococcus aureus ATCC 6538. e zone of inhibition obtained around the wells was expressed in millimeters (mm).

Turkey Meat Burgers Production.
e turkey meat burger samples were prepared and consisted following a homemade formulation using these ingredients: turkey breast meat (75%), water (10%), modified starch (8%), salt (2%), and spices (5%). e turkey breast meat was partially replaced by IFC at 3, 5, and 10%. Controls without IFC were used in all assays. e percentages of water and spice additives were unchanged compared to the control sample. e main difference consisted in decreasing meat content and increasing fiber content (Table 1).
Fresh breast meat (about 2 kg) was minced using a grinder with 3 mm plate (ME60514A, Moulinex, France) and it was packed on four portions and stored at 4°C. Ingredients and IFC were blended manually in a bowl during 5 min, to ensure uniform distribution of the added ingredients. en, turkey meat burgers (around 50 g) were formed using a conventional burger maker, wrapped in a transparent polyvinyl chloride film. Finally, the turkey burger samples were stored in a refrigerator at 4°C until analyses were performed after 24 h of storage. All analytical determinations were performed in triplicate (n � 3).

Physicochemical Analysis.
e pH, water activity, and color were determined using the same method for DSP and IFC analysis. All analytical determinations were performed in triplicate (n � 3).

Texture Measurement.
All instrumental texture analyses were done on samples stored at least for 24 h at 4 C according to Ayedi et al. [32]. Instrumental Texture Profile Analysis (TPA) was analysed using texturometer (Universal Texture Analyser, TA plus, LLOYD Instruments, England) assisted by a computer at room temperature (25°C). e TPA technique is a two-cycle compression test to determine texture characteristics: hardness, cohesiveness, elasticity, adhesiveness, and chewiness. e samples were cut into a cylindrical shape with predefined size (2 cm high × 2 cm diameter) from the central region of the burgers. e test was done using a cylindrical probe of 25 mm diameter. e probe was aligned in the middle of the sample. e probe compressed the burgers sample by 50% of its original height.

Sensorial Analysis.
e sensory acceptance of burgers samples was conducted by 36 panelists. e panel consisted of students and academic staff of the Biological Department of the National School of Engineers of Sfax (ages ranged from 23 to 50 years). e burgers were cooked on a hot plate previously greased with olive oil temperature at 175 ± 5°C for 3 min, cooled for 2 min, and served to panelists. is methodology was adapted from Sayago et al. [30]. e burgers samples were given in a homogeneous way to the panelists and identified with a three-digit random number. Panel members were later asked to rate the likeness on appearance, color, flavor (odor and taste), texture, and the overall acceptability of the samples. e rating was done through a five-point hedonic scale, with 1 � dislike extremely, 3 neither like nor dislike, and 5 � like extremely. Experiments were conducted in an appropriately lighted room and a mean score was estimated for each product. Burgers formulated with IFC were compared to the control to predict their acceptance by consumers.

Statistical Analysis.
All analytical determinations were performed in triplicate. Values were expressed as the mean ± standard deviation (SD). Statistical analyses were Journal of Food Quality carried out using a SPSS software for Windows, version 20.0 (Statistical Package for the Social Sciences, e Predictive Analytics Company, Chicago, IL, USA). Analysis of variance (ANOVA) was performed using Tukey's test to determine significant differences between the samples (P < 0.05).
To group samples based on physicochemical analysis (pH and Aw), instrument color (a * , b * , and L * ) and texture (chewiness, hardness, elasticity, cohesiveness, and adhesiveness) parameters, and sensory characteristics (color, flavor, appearance, texture, and overall acceptability), all variables were autoscaled prior to chemometrics application. XLSTAT software for Windows (v.2014.1.08, Addinsoft, New York, USA) was used. Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) were performed to distinguish between treated and untreated samples. For all samples, dendrograms were recognized to find a two-dimensional projection of the similarity or dissimilarity of the entire samples set.

Physicochemical Composition.
e physicochemical compositions of the DSP and IFC are presented in Table 2.
e yield of the insoluble fiber extraction from DSP was the highest (P < 0.05) with 67.52%. ese two fractions were characterized by a neutral pH ranged from 6.595 to 6.945 and a low Aw varied from 0.180 to 0.258 as lower than 0.6. Hence, these results showed that all microbiological activities were blocked and indicated that the DSP and IFC might contribute to a long-term conservation and an excellent protection against all bacterial and fungal alterations [33]. Moreover, this neutral pH of date kernels allows their integration in several food formulations.
In addition, DSP showed an important level of total carbohydrates and lower than those of IFC (82.55 versus 88.85%, respectively). DSP fat, protein, and ash contents were higher (P < 0.05) than those of IFC (9.42, 6.20, and 1.78 versus 5.94, 3.81, and 1.15%, respectively). ese significant differences of these compounds' values could be explained by their solubility in alkaline water. ese results were similar to those obtained in our previous study which we carried out for the same fractions of Tunisian Deglet Nour seeds [3]. For DSP, Gharib et al. [34] and Gökşen et al. [35] have found similar results for seeds from other date Sukkari and Safawi, Sagai, and Mabroom varieties, respectively.
Minerals contents of DSP and IFC were presented in Table 2. Significant difference (P < 0.05) was registered between the two fractions for all minerals. DSP had a higher mineral content compared to these of IFC. is difference could be due to solubility of minerals during alkali water extraction. For both fractions, potassium was the highest mineral content, followed by phosphorus, magnesium, calcium, sodium, and then iron and zinc. ese results are in agreement with those found by Bouaziz et al. [3] and Besbes et al. [6] for Tunisian Deglet Nour date seeds, but slightly lower than those reported by Bouhlali et al. [36], Gharib et al. [34], and Gökşen et al. [35] for Moroccan, Egyptian, and Saudi date seeds from other varieties, respectively. Certainly, those differences may be attributed to the variability of the studied cultivars. erefore, these physicochemical results of DSP and IFC confirm that these fractions may provide important health benefits and could be valued as a potential source of dietary fiber and minerals with large spectrum of use in the food industries.
CIELAB coordinates (L * , a * , and b * ) of DSP and IFC have presented a clear brown color with slightly dark lightness (P > 0.05) and redness values (P < 0.05) (L * � 31-30; a * � 14-12, respectively) ( Table 1). b * color parameter of DSP was lower (P < 0.05) compared to that of the IFC (10.01 versus 12.96, respectively). Indeed, the positive value of b * indicated the yellowness in the DSP and IFC.
ese results show that the DSP color was slightly brown compared to that of IFC. is difference in the color of DSP and IFC could be due to the DSP pigments solubility during the alkali water extraction. ese results were in agreement with these found by Bouaziz et al. [37] who obtained a redbrown taint in the neutral and alkaline pH values of the water extract of date seeds from Deglet Nour and Allig varieties. Globally, our results can provide information in the impact of the date seed fiber color over color of the product where it will be added. erefore, seeing this light brown color of both fractions DSP and IFC; it is easy to add to many food products especially meat products.

DSP and IFC Functional Properties.
Functional properties described by water-holding capacity (WHC), oilholding capacity (OHC), and swelling capacity (SWC) are presented in Table 3. No significant difference (P > 0.05) was  Journal of Food Quality observed for the WHC of both fractions. WHC of DSP was slightly (P < 0.05) higher than those of date seeds from Deglet Nour found by Bouaziz et al. [3] (5.80 versus 3.23 g water/g sample, respectively). Moreover, the WHC of IFC was higher (P < 0.05) than those of the fiber concentrate from Deglet Nour seeds reported by Bouaziz et al. [3] (5.54 versus 3.01 g water/g sample, respectively). Significant differences (P < 0.05) were observed for the OHC and SWC properties between DSP and IFC. is later presented a higher (P < 0.05) OHC compared to those of DSP (5.18 versus 2.96 g oil/g sample, respectively). However, SWC of IFC was slightly lower (P < 0.05) than those of DSP (4.72 versus 6.37 ml/g sample).
WHC, OHC, and SWC of DSP were mainly attributed to the presence of soluble and insoluble fiber contents (total carbohydrates � 82.55%). Nevertheless, for the IFC, these three technological parameters were essentially due to only the presence of high insoluble fiber contents (total carbohydrates � 88.85%). WHC and OHC of DSP were slightly (P < 0.05) higher than those of date seeds from Deglet Nour found by Bouaziz et al. [3] (5.80 and 2.96 versus 3.23 g water/g sample and 1.14 g oil/g sample, respectively). Moreover, the WHC and OHC of IFC were higher (P < 0.05) than those of the fiber concentrate from Deglet Nour seeds reported by Bouaziz et al. [3] (5.54 versus 3.01 g water/g sample and 5.18 versus 2.51 g oil/g sample, respectively). ose differences may be attributed to the variation of the fiber particle sizes, in the chemical composition and also the variability of the studied date cultivars. Goksen et al. [35] reported that WHC of date seeds from three Saudi cultivars (Safawi, Mabroom, and Sagai) was ranged between 5.96 and 6.87 g/g DM and was slightly higher than those of DSP. SWC of Safawi seeds was the highest (7.01 g/ml), followed by Mabroom (5.94 g/ ml) and Sagai (5.89 g/ml). Hodge and Osman [38] reported that flours with high water holding capacity have more hydrophilic constituents such as fibers. Apple, wheat, sugar beet, pea, and carrot fibers represented a WHC ranging from 2.5 to 10 g/g dry weight.
In addition, the high hydration properties of these DSP and IFC suggest that they can be used as functional ingredients to improve the sensory properties of formulated products, to reduce syneresis, modify texture, viscosity, and reduce calories of foods. Hydrophobic constituents are the main reason for OHC. It is related to the chemical composition, the nature, and structure of the fiber and the affinity of the fiber molecule to oil [39]. ese properties of these byproducts allow the development of functional foods  with high value-added and attract the attention of consumers.

Biological Activities of Deglet Nour Date Seeds Powder (DSP)
(1) Total Phenolic Contents (TPC). TPC and antioxidant activity using two assays', DPPH and ABTS, scavenging activities are presented in Table 4. TPC of DSP was relatively highest (P < 0.05) with 47.66 mg GAE/g DM. ese results were similar compared to these found by Bouhlali et al. [36] who studied TPC of three Moroccan date seeds: Boufgous, Bousthammi, and Majhoul varieties recorded at 26, 53, and 30 mg GAE/g DW, respectively. However, Bouaziz et al. [37] reported that the TPC of Deglet Nour date seeds was 38.34 mg GAE/g. Amany et al. [10] reported that the TPC of date pits from Khalas variety extracted by water was around 15 mg GAE/g DW. e differences in TPC between ours results and the previous study could be explained by numerous factors. e extraction process used in the other studies differs from ours, and this may lead to differential extraction of polyphenols, the variability of the studied cultivars, and the climatic conditions of growing date palms. ese results suggest that Deglet Nour date seeds are an excellent source of phenolic compounds for multiple phytotherapeutic virtues and could be a great source of natural antioxidant.
(2) Evaluation of the Antioxidant Activity of DSP. e DPPH and ABTS radical scavenging activity were 5.74 and 7.03 mmol TE/100 g DW, respectively (Table 4). ese results were in agreement with these found by Goksen et al. [35] and Bouhlali et al. [36]. ese authors reported that the ABTS activities ranged between 4.80 and 8.02 mmol TE/ 100 g for three Moroccan date seeds (Boufgous, Bousthammi, and Majhoul). Goksen et al. [35] stated that the DPPH activities of three Saudi date seeds (Safawi, Sagai, and Mabroom) ranged from 4.84 to 6.17 mmol TE/100 g. ese antioxidant properties are also much needed by the food industries in order to find possible alternatives to replace chemical products or synthetic preservatives as antioxidant agents. In this case, DSP gave interesting results such as ability to neutralize free radicals and fought against fatty acid oxidation.
(3) Evaluation of the Antibacterial Activity of DSP. As can be seen in Table 4, the DSP extract was found to have relatively moderate antimicrobial activities against all tested microorganisms. Ethanol extract from DSP demonstrated strong antagonism against Bacillus cereus, Staphylococcus aureus, and Escherichia coli (Table 4), at which the highest zone of inhibition is 15 mm, 14 mm, and 13 mm at 5 mg/ml concentration, respectively. Sundar et al. [40] reported that the antibacterial activity of acetone extract from date seeds showed the highest zone of inhibition of 20 mm and 17 mm against E. coli and B. cereus at 1 mg/ml concentration. In addition, DSP showed to have a relatively weak antibacterial activity against Salmonella typhimurium, Pseudomonas aeruginosa, and Listeria monocytogenes, at which the inhibition zones were 11 mm for these three bacteria. e antibacterial activities of DSP extract could be related to the presence of phenolic compounds that are known as antimicrobial agents against several pathogens Gram positive and Gram negative [41].

Physical Parameters
(1) Changes in pH and Water Activity. Water acidity (Aw) and pH of various turkey burgers samples were determined after 24 h of conservation at 4°C (Table 5). Results showed that the adding of IFC to turkey burgers had no significant effects (P > 0.05) on Aw and pH whatever the rate of concentration (3, 5, and 10%). e Aw of formulated burgers with IFC was slightly lower to those of the control (0.94 versus 0.95, respectively). In addition, IFC incorporation had not affected the pH of the turkey burgers samples. Likewise, pH of formulated burgers was slightly acidic and ranged from 5.89 to 5.98 for all samples. e obtained rO values were within the specific range for this type of meat. ese values were similar to white muscles of White Emperor turkeys, namely, 5.99 in breast meat of male and 5.71 in female turkey [42].
(2) Changes in Color. Turkey meat products' color is one of the main factors by which consumers judge their acceptability. e incorporation of no turkey ingredients like IFC in burgers can cause changes in turkey burgers. Table 5 presented the changes in color parameters of the raw turkey burger samples prepared with 0, 3, 5, and 10% IFC after 24 h of conservation at 4°C. Color measurement showed significant changes (P < 0.05) of color between all different burger samples. Indeed, the IFC addition decreased lightness (L * ) and yellowness (b * ) but increased redness (a * ) compared to the control for all turkey burgers formulated and as function of the concentration levels. In fact, for example, L * ,  Journal of Food Quality a * , and b * changed strongly from 41, 4.32, and 17.38 for the control to 27.33, 6.80, and 8.60 for burger formulated with 10% IFC, respectively. ese results could be explained by the presence of higher pigmentations in IFC. ese pigments were mainly polyphenols from the Deglet Nour date seeds powder (DSP). Similar effects have been observed using a red grape pomace (Vitis vinifera) in raw chicken burger products [30] and in raw pork using grape seed extract [43]. Furthermore, the obtained results are in agreement with those previously reported by Bouaziz et al. [37] for the enriched bread by defatted date seeds from Deglet Nour and Allig varieties that the bread color changed with the increasing of the date seed powder corporation levels. Despite these variations of color parameters, the formulated burgers with IFC retained overall their characteristic red color.

Changes in Texture.
Texture is considered as the major determining parameter of food acceptability [39]. Indeed, sensory properties are highly correlated with instrumental texture parameters [44]. Texture parameters (hardness, cohesiveness, adhesiveness, elasticity, and chewiness) of the raw turkey burger samples were obtained 24 h after conservation at the temperature of 4°C, and the results are shown in Table 6. Hardness of turkey burgers formulated with IFC was significantly higher compared to the control (P < 0.05) (4-6 N versus 2 N, respectively). e addition of IFC induced the increase of hardness, which is a function of the IFC concentration. e increase of hardness for formulated burgers could be attributed to the IFC fiber contents to give strength to the network. ese results indicated that the replacement of meat by IFC changed significantly the structure and avoid new rearrangements of liaison protein-fiber providing a more resistance of deformation.
However, no significant difference (P > 0.05) was observed for turkey burgers elasticity and cohesiveness for each IFC concentration and compared to the control. Elasticity and cohesiveness of burger samples were around 3 mm and 0.286-0.325, respectively.
Furthermore, raw burger adhesiveness and chewiness values were statistically different (P < 0.05) to the control samples and were a function of the IFC incorporation rate. Adhesiveness increased from 0.861 N for the control to 2.537 N for the B10IFC but chewiness values decreased from 2.269 Nmm for the control to 0.366 Nmm for B10IFC with the increase of IFC concentration.
All these textural modifications can be explained by the influence of the presence of IFC on the formulated turkey burgers. IFC-muscle proteins interaction leads to a change in texture and microstructure of the formulated burgers. Fernandez-Gines et al. [45] demonstrated that albedo addition caused an increase in hardness, regardless of the added dose, and reported similar results. e increase in hardness perception was higher in bolognas with raw albedo than cooked albedo. Some authors also reported that inclusion of fibers in various meat products increased hardness. Lee et al. [46] reported that doughnut containing soybean hulls flour increased hardness without affecting any sensory quality parameters. Moreover, Ayedi et al. [32] noticed the increase of the hardness of the formulated sausages with Carrageenan at different concentrations and varied from 12 N to 16 N. However, the addition of apple, peach, or orange fibers decreases the hardness of the products. Springiness, adhesiveness, and cohesiveness follow an irregular behavior without compromising added fiber levels. Huber et al. [47] reported that the hardness of chicken burgers formulated with some vegetable was mostly significantly lower than that of the control sample. e elasticity and the cohesiveness of the burger samples were statistically equal among each formulation and compared to the control.
It should be noted that IFC addition to turkey burgers improved certainly the functional and nutritional quality as well as the health benefits with some significant modifications in the textural properties.

Sensorial Properties.
e impact of the IFC addition on sensory attributes of turkey burgers after 24 h storage at 4°C is presented in Table 7. Results showed that the formulated turkey burgers with 10% IFC were significantly (P < 0.05) less appreciated by panelists against control for all sensorial characteristics. Burgers samples formulated with 10% IFC (B10IFC) had the lowest (P < 0.05) average score of appearance, color, flavor, and texture compared to the control (2-2.5 versus 3.2-4.10, respectively). However, no significant differences (P > 0.05) were recorded for all textural parameters of formulated burgers with 3% IFC (B3IFC) and 5% IFC (B5IFC) compared to the control. ese results could be mainly due to the coloration induced by the red-brown pigments, taste, and the particle sizes of the IFC. e highest (P < 0.05) texture score was obtained for B5IFC followed by B3IFC and control (4.10, 3.90, and 3.20, respectively) samples. In addition, B5IFC had a slightly (P < 0.05) higher flavor score compared to these of control (3.90 versus 3.30). B5IFC was appreciated by the panelists and more deemed superior compared to the control. Interestingly, B5IFC had the better overall acceptability (P < 0.05) with an average rating of 3.72. erefore, formulated burgers with 3 and 5% IFC acted positively on the sensory quality of meat samples and improved their overall acceptance. is justifies the important effect of the insoluble fibers added for their technological properties. Globally, sensory analysis shows that IFC presence has no significant effect on burgers up to 5% of concentration in appearance, color, texture, flavor, and the overall acceptability. Remarkably, B5IFC was the best for all sensory characteristics and most appreciated by panelists.   , instrument color (a * , b * , and L * ) and texture parameters (chewiness, hardness, elasticity, cohesiveness, and adhesiveness), and sensory characteristics (color, flavor, appearance, texture, and overall acceptability) within score plot of untreated (control) and different treated (B3IFC, B5IFC, and B10IFC) meat samples.

Correlations between Physicochemical Analysis, Instrument Color, and Texture Parameters and Sensory Characteristics: Chemometric Approaches.
To correlate all traits, eigen values, and cumulative variance for all factors of each sample, PCA and HCA were utilized. PCA analysis was employed to accomplish an overview of the general similarities and differences between the four samples. Figure 3 reveals the results of PCA for the 15 variables shown in Tables 5-7. It can be observed that the explained variance of the first two components was 92.29%. Loadings plots (Figure 3) presented a positive relationship between sensory attributes (appearance, color, and overall acceptability) and instrumental color (a * and b * ) measurements. ese findings are in good agreement with the arguments supporting the impact of redness (a * ) and yellowness (b * ) on sensory parameters [48]. At the same time, sensory color intensity varied in the same direction from the raw luminosity (L * ) and yellowness (b * ) scales. On the other hand, a * for raw meat, redness, was positively correlated between the instrumental (hardness, elasticity, and adhesiveness) data, indicating the fact that it is a parameter with great influence on the differentiation of the raw color. Results from the instrumental analyses of texture (hardness, elasticity, and adhesiveness) and those from all sensory analyses were negatively correlated as can be seen between the instrumental analysis for hardness and the sensory analysis for tenderness [49]. Factor scores plot ( Figure 3) presented a great difference between the four samples. Overall, B10IFC samples were located in the positive axis of the first PC associated with high scores of hardness, elasticity, and adhesiveness and a * . However, control samples, situated on the negative axis of PC2, correlated negatively with sensory attributes (color, appearance, and overall acceptability), chewiness, b * and L * . Finally, no significant correlation concerning relationship between cohesiveness and sensory parameters (flavor and texture) of (B3IFC and B5IFC) was observed.

Correlations by HCA.
HCA was determined with a view to differentiate all samples based on the mentioned variables. In fact, especially due to moderate levels of sensory attributes, instrumental textural, and color parameters and taking into account their similarities, the dendrogram from HCA ( Figure 4) represents three groups [Control, (B3IFC and B5IFC), and B10IFC]. Likewise, Nishad et al. [50] and Fourati et al. [51] reviewed the application of HCA for quality control and authentication of meat balls treated with nutmeg and citrus peel extracts and minced beef meat added with pomegranate peel extract, respectively.

Conclusion
Physicochemical analysis of date palm seeds obtained from Deglet Nour variety showed a great richness on carbohydrates and phenolic compounds that could be valorized by including seeds powder or its insoluble extract in food formulation enhancing nutritional, technological, and stability of produced food. DSP and IFC proved their efficient use as innovative and attractive additives in meat processing. e influence of IFC addition on pH, Aw, color, textural parameters, and sensory properties of turkey burgers were studied. Burgers formulated by IFC as a source of dietary  Figure 4: Agglomerative hierarchical cluster analysis (HCA) of physicochemical analysis (pH and aw), instrument color (a * , b * , and L * ) and texture parameters (chewiness, hardness, elasticity, cohesiveness, and adhesiveness), and sensory characteristics (color, flavor, appearance, texture, and overall acceptability) of untreated (control) and different treated (B3IFC, B5IFC, and B10IFC) meat samples. fiber lead to a strongly change in raw burgers properties at 10%. Moreover, compared to control samples, up to 5% IFC concentration, the formulated burgers had similar textural properties and better sensory parameters such as texture, flavor, and an overall acceptability. anks to these results, it could be concluded that burgers formulated by 5% IFC are the most appreciated product by consumers. is substitution reduced the production cost without affecting sensory characteristics. By using chemometrics technique (PCA and HCA), all data offers valuable information to separate all samples and connect physicochemical properties to sensory and instrumental color attributes through correlation models. erefore, IFC might be used as natural ingredient in functional meat making, in response to consumer needs.

Data Availability
e data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest
e authors declare that there are no conflicts of interest regarding the publication of this paper.