Synthesis of Isomaltooligosaccharides (IMOs) from Sweet Potato Starch by Simultaneous Saccharification and Transglycosylation Using Saccharomyces cerevisiae Var. diastaticus BE 134 to Improve Purity of IMOs

This study developed a simple two-step procedure to produce isomaltooligosaccharides (IMOs) from low-cost sweet potato starch (SPS). Eﬀect of various reaction parameters on the steps of the synthesis process of IMOs was systematically investigated. The results show that Spezyme Xtra enzyme was the most suitable for the liquefaction step. The oligosaccharide components’ contents, including G 1 –G 10 and G 2 –G 6 , reached 73.95 ± 0.02% and 49.24 ± 3.19%, respectively, after liquefaction. The simultaneous sacchariﬁcation and transglycosylation (SST) reaction of SPS followed the liquefaction after the α -amylase activity was deactivated. This reaction was simultaneously treated by β -amylase, pullulanase, and α -transglucosidase. The eﬀect of various reaction parameters, consisting of solution pH, reaction temperature, enzyme dosage, and reaction time, on the SSTreaction to synthesize IMOs from SPS was fully studied. The results showed that the highest concentration of IMOs (IG 234 ) reached 68.85 ± 1.82g/L at the optimal condition. The puriﬁcation of pristine IMO was performed by adding Saccharomyces cerevisiae var. diastaticus BE 134 yeast cells at the ﬁnal step of the procedure. In particular, the SST reaction for the synthesis of IMOs from SPS shortened SST reaction time by three times compared with other three-step synthesis procedures of IMOs. These ﬁndings show that the SPS-derived IMOs can be applied as a novel and inexpensive prebiotic healthcare product for human gastrointestinal health, dieters, and diabetics.

e IMOs are the potential prebiotics to help improve the beneficial microflora in the body. For example, the Bifidobacteria in the feces were found to significantly increase when human applied IMOs derived from different sources [10][11][12][13], as well as increasing reproduction of Lactobacilli [10,11] while inhibiting nonbeneficial bacteria such as Clostridium perfringens in both mice and human intestinal tracts [10] when the IMOs were used. On the other hand, E. coli and other pathogenic bacteria could not metabolize IMOs [14]. e IMOs have been shown to have a low glycemic index [15,16] and anticonstipation effects [17] and reduced amount of cholesterol in the blood.
Most commercial IMOs have been enzymatically produced from a wide range of starches through transglycosyl action [3]. e conventional synthesis of IMOs from starch is involved in a three-step procedure of liquefaction, saccharification, and transglycosidation in separation. Firstly, the starch is decomposed into α- (1,4)-linked α-D-glucooligosaccharides using α-amylase, pullulanase, and β-amylase. In the next step, the α- (1,4)-linked α-Dglucooligosaccharides continue to be converted into α-(1,6)linked oligosaccharides using α-transglucosidase [18]. en, in the transglycosidation step, transglucosidase usually carries out two tasks: (1) the hydrolysis of α-D-glucooligosaccharides and (2) transfer of the glucosyl group to 6-OH of glucose. At the end of process, beside the desirable products of isomaltose and panose which are formed from the transglycosidation of glucosyl to D-glucose and maltose, respectively, the process also generates a large amount of glucose and α- (1,4) oligomers (mainly maltose) as byproducts [7,19]. ese impure by-products need to be removed from obtained IMO products. e transglucosidase was reported to be present in various fungi, such as Aspergillus niger [1], Aureobasidium pullulans [20], Aspergillus carbonarius [21], ermoanaerobacter thermocopriae [22], and Aspergillus nidulans [23]. However, the transglucosidase was extracted from these types of fungi for low purity of IMOs. Moreover, the neopullulanase in Bacillus subtilis [24] was reported to contain function of a-(1,6)-transglycosylation which can combine with α-amylase for the synthesis of high-purity IMOs from starch. Improved efficiencies of IMO synthesis have been reported by enzymes combination used in the manufacturing process. e action of simultaneous catalysis by α-amylase and BE followed by simultaneous β-amylase and α-transglucosidase using cassava starch as substrate liberated high contents of isomaltose, isomaltotriose, and panose [25]. In addition, IMO and oligodextrans were also synthesized from sucrose using the enzymes dextransucrase and dextranase [26]. e coupling of Fungamyl and α-transglucosidase improved productivity of IMO production and alleviated the inhibition of glucose to action of α-glucosidase in transglycosylation step [27]. erefore, the three kinds of β-amylase, pullulanase, and transglucosidase were simultaneously supplemented into SST reaction with desire to shorten time required for highest IMOs concentration in this study. e strategy was based on the combination of β-amylase and pullulanase for starch saccharification obtaining higher contents of maltose and maltotriose with low residual maltodextrins, which resulted in elevated levels of substrates for transglycosylation. At the same time, the presence of transglucosidase enzyme in the solution immediately resulted in transglycosylation and generated IMOs, in which α-(1,6)-linkages are formed after transglycosylation that is not attacked by pullulanase because the products of IMO synthesis process do not meet the properties of the substrates of pullulanase [28]. In particular, in the final step of synthesis procedure of IMOs, the Saccharomyces cerevisiae var. diastaticus BE 134 yeast cells were applied to eliminate undesirable sugars such as glucose, maltose, and maltotriose to improve further purity of obtained IMOs.
It is clear that a three-step synthesis procedure of IMOs from various starch sources exhibited many drawbacks due to consuming much time and cost for deactivation of enzymes activities and adjusting reaction parameters after the reactions were finished to be suitable with following reactions. erefore, development of a two-step simple synthesis procedure of IMOs should be applied to overcome the aforementioned drawbacks of the three-step procedure.
Due to possessing many advantages, the studies to produce IMOs from various sources have gained increasing attention by scholars. Beside the IMOs that have been found in traditional products such as honey [29], miso [30], and sake [31], the IMOs have been produced from a wide variety of starch sources such as tapioca starch [25,32], corn starch [4], rice starch [1], banana starch [9], sugarcane plants [33], and chestnut starch [7]. e sweet potatoes are one of the most popular starch sources around the world. In particular, the sweet potatoes were grown all over agriculture land of Vietnam with abundant concentration and very cheap price. e Food and Agriculture Organization of the United Nations (FAO) reported that the amount of sweet potatoes which was produced in Vietnam in 2019 was about 1,402,350 tons, accounting for approximately 0.98% of the total world production [34]. In this study, the Hoang Long sweet potatoes grown in infertile soil of Bac Giang province where there is tropical monsoon climate with the annual average temperature, rainfall, and air humidity of 23.2°C-23.8°C, 1400-1730 mm, and 83 to 84%, respectively, were used to produce IMOs. Besides, the main sweet potatoes' functional components were starches, vitamins, minerals, and amylose which have been proved as health-promoting functions [19]. ese components are suitable for the production of IMOs. Hence, the sweet potato starch has potential as a quality raw material for production of IMOs. However, to the best of our knowledge, the production of IMOs from the sweet potatoes has not been investigated up to now, especially the production of IMOs from sweet potato starch by a two-step procedure using simultaneous saccharification and transglycosylation.
erefore, the aim of this study was to synthesize the IMOs product derived from sweet potato starch as functional food ingredient for human gastrointestinal health, dieters, and diabetics. To reach this aim, a simple, inexpensive, and highly effective two-step synthesis procedure of IMOs from sweet potato starch was developed using the simultaneous saccharification and transglycosylation. Besides, effects of various reaction parameters, including kinds of enzyme preparation, enzyme dosages, solution pH, reaction temperature, and reaction time, on the overall synthesis procedure of IMOs from SPS were systematically investigated to choose the optimal condition for production of IMOs. e obtained products of the synthesis procedure of IMOs were analyzed using HPLC-RID.
Saccharomyces cerevisiae var. diastaticus BE 134 yeast cells were acquired from Fermentis by Lesaffre.

Total Carbohydrate Concentrations.
Total carbohydrate concentrations were determined based on the study of Dubois et al. [35]. First, an exact amount of 10 mg sample was transferred into test tube with simultaneously adding 0.2 mL of ethanol (80°) and 1.0 mL of DMSO (90%) to the mixture. e test tube was then completely shaken to homogenate sample. Afterwards, the test tube was stood for 10 min at room temperature (25 ± 2°C). In the next step, a 2.0 mL of boiling double distilled water was supplemented into the test tube and boiled for 15 min. e solution was then cooled down and distilled water was added until reaching exactly 10 mL of solution. e obtained solution was continuously diluted 10 times to achieve solution B. 1.0 mL of solution B was pipetted and transferred into the test tube. Simultaneously, 1.0 mL of phenol and 5.0 mL of concentrated H 2 SO 4 were added to this test tube. e reaction that occurred in this test tube was maintained for 20 min of contact time. e solution was finally analyzed by UV-Vis spectrophotometer (photoLab 6600, Germany) at λ � 490 nm for determination of total carbohydrate concentrations.

α-Amylase Assay.
Amylase activity of α-amylase preparation was determined according to Ceralpha method which was instructed by Megazyme [36]. e principle of the method is based on hydrolysis of nonreducing-end blocked p-nitrophenyl maltoheptaoside (BPNPG7) by α-amylase to form p-nitrophenyl maltosaccharide. After that, α-glucosidase presence in solution was activated to participle this hydrolysis reaction to form glucose and free p-nitrophenol. At the end of reaction, reaction solution would turn into yellow color. Finally, the p-nitrophenol was determined based on standard coefficient [36].
In this study, the procedure for assay of α-amylase was performed as follows: firstly, a 0.2 mL of BPNPG7 substrate was transferred into test tube and preincubated at 40°C for 5 min. In parallel, the diluted α-amylase HR reagent solution (unbuffered) was also preincubated at 40°C for 5 min. In the next step, an above preincubated 0.2 mL of the diluted α-amylase preparation was directly added to the bottom of the test tube containing BPNPG7 substrate. e test tube was continuously incubated at 40°C for exactly 10 min. At the end of the 10 min incubation period, an exact amount of 3.0 mL of stopping reagent was supplemented in the mixture and vigorously shaken in test tube. Finally, the result solution and blank sample were measured using UV-Vis spectrophotometer (photoLab 6600, Germany) at λ � 400 nm. One enzyme activity unit is defined as the amount of enzyme, in the presence of excess thermostable α-glucosidase, required to release one micromole of p-nitrophenol from BPNPG7 in one minute under the defined assay conditions. e activity unit is termed as Ceralpha Unit (CU).

β-Amylase
Assay. β-Amylase activity was determined according to betamyl-3 method [37]. Firstly, the betamyl-3 substrate (PNPβ-G3) solution was preincubated at 40°C for approximately 5 min. At the same time, 0.2 mL of diluted enzyme solution was transferred into test tube and preincubated at 40°C for approximately 5 min. In each test tube containing diluted enzyme, 0.2 mL of PNPβ-G3 was supplemented. e tube was then shaken completely and incubated at 40°C for exactly 10 min. At the end of the 10 min incubation period, 3.0 mL of stopping reagent was added to the test tube and stirred vigorously to reach homogenous mixture. Finally, the result mixture and blank sample were measured using UV-Vis spectrophotometer (photoLab 6600, Germany) at λ � 400 nm. One unit of enzyme activity is defined as the amount of the enzyme β-amylase required to release 1 micromole of p-nitrophenol from PNPβ-G3 per 1 min under the defined assay conditions, and it is termed as betamyl-3 unit. e conversion from betamyl-3 activity units to IU units was followed according to Megazyme's instructions.

Pullulanase Assay.
e principle of pullulanase analysis method is followed by Megazyme's instructions [38]. In this study, to analyze pullulanase, firstly, both pullulanase and Red Rullulan substrate were preequilibrated at 40°C for 5 min. en, add 1.0 mL preequilibrated enzyme solution to 0.5 mL of preequilibrated Red Pullulan solution. Stir the mixture and incubate at 40°C for exactly 10 min. Stop the reaction by adding 2.5 mL of ethanol (95% v/v) and shaking vigorously for 10 seconds. Allow the reaction tubes to equilibrate at room temperature for 10 min. e tube was centrifuged at 1000g for 10 min and the supernatant was collected to measure pullulanase activity at λ = 510 nm. e blank sample was prepared by adding ethanol to the Red Pullulan substrate before adding the enzyme. e activity of pullulanase is determined by reference to the standard curve (provided by [38]).

Transglucosidase Assay.
Transglucosidase activity was determined using maltose as substrate which was adapted from the previous study [27]. e transglucosidase enzyme was incubated with 1% (w/w) maltose solution at 40°C in 0.5 mM sodium acetate buffer (pH 4.0) for 15 minutes. e reaction was stopped by boiling the mixture for 10 min. e amount of released glucose was measured using GOD-POD kit (Megazyme). One unit of enzyme activity is defined as the amount of the enzyme that produces 1 μmol of glucose per minute under the aforementioned conditions.

Preparation of Sweet Potato Starch.
e sweet potato starch (SPS) was prepared from fresh tubers immediately after harvest. First, the sweet potatoes were cleaned with water several times to remove dirt. Next, the sweet potatoes were cut into small sheets using knife and ground by blender. e ground sweet potatoes were then sifted through 60, 100, and 200-mesh sieves to obtain pure starch. e residue was removed. e solution containing starch was then left to settle for 6 hours. e resulting solution was decanted to gain pure starch and remove water as well as impurities on the surface of the settled starch. Settling process was repeated 3 times. Finally, wet starch was obtained and dried at 40°C until its humidity reached about 10%. e starch extraction rate was 11.58% calculated on fresh weight basis. e products were stored in plastic bags at 4°C for next experiments.

Synthesis Procedure of IMOs from Sweet Potato Starch.
e synthesis procedure of IMOs from sweet potatoes is illustrated in Figure 1.
Processing of IMOs from sweet potatoes is described in detail as follows.

Liquefaction.
Firstly, the dried sweet potato starch (SPS) was dissolved in acetate buffer at pH of 5.8 to reach 25% (w/v) of starch slurry. In the next step, four enzyme preparations of Spezyme Xtra, Spezyme Alpha, Termamyl SC DS, and Liquozyme SC DS were separately supplemented into reactor with content of each enzyme of 1.0 CU/g dried SPS. e reactor was maintained about 80-85°C for 30 min reaction time. At the end of liquefaction reaction, lactic acid was added to reactor to adjust solution pH to 3. A total of 1.0 mL of the liquefied fluid was taken out to measure OS components at interval time of 30 min using HPLC-RID.

Simultaneous Saccharification and Transglycosylation.
After liquefaction, the liquefied mixture was adjusted to pH of 5 by NaOH 5N. 3.0 U/g β-amylase, 0.8 U/g pullulanase, and 10.0 U/g transglucosidase were then added to the mixture simultaneously. e mixture was vigorously shaken at 50°C for 12 h. e sample was withdrawn after 12 h for determination of types and contents of the sugar components. e types and contents of the sugar components in the sample, including IG 2 -IG 4 , were determined using HPLC-RID. Stopping the reaction was performed by boiling the mixture for 10 min.

Purification of IMOs from Sweet Potato Starch.
Because the IMOs product obtained from the simultaneous saccharification and transglycosylation (SST) step by β-amylase, pullulanase, and α-transglucosidase contained undesirable sugar components such as glucose, maltose, and maltotriose, it is necessary to remove these undesirable byproducts. In this study, Saccharomyces cerevisiae var. diastaticus BE 134 yeast cells were applied for the removal of these undesirable sugars. Yeast cells after being obtained from Fermentis by Lesaffre were activated at 28°C for 15 min. In the next step, the yeast cells were added to the test tube containing IMOs solution with cells densities of Saccharomyces cerevisiae var. diastaticus BE 134 of 2 × 10 8 cells/mL [1]. en, the test tube was thermally stabilized at 28°C. Afterwards, a 1.0 mL of sample in this test tube was taken every 12 h. e test tube was then heated to 95°C for 10 min to kill all yeast cells. e mixture was centrifuged at 10000g for 15 min to remove dead yeast cells and the supernatant was collected to qualitatively determine the undesirable sugars content using in-Layer Chromatography (TLC). At the point of reaction time when contents of glucose, maltose, and maltotriose are used up by yeast cells, the resulting samples solution is used to quantify IMOs concentration by HPLC-RID. en, a sodium acetate buffer (pH 5.8) was added to this Falcon tube to reach the final concentration of suspension sample of 25% (w/v) with the total volume of 30 mL. e α-amylase preparation was supplemented into the mixture with a content of 1 CU/g dried SPS. e mixture was heated for 25 min. e reaction temperature was maintained at 80°C for the mixture with Spezyme Xtra and Liquozyme SC DS and at 85°C for reaction with Spezyme Alpha and Termamyl SC DS. After 30 min of reaction time, the enzyme was immediately deactivated by concentrated lactic acid at pH of 3.0. Finally, the obtained mixture after the liquefaction was diluted to the suitable concentration with double distilled water to determine the oligosaccharides contents by HPLC-RID and the residual starch content by the phenol-sulfuric acid method.

e Experiment to Investigate the Effect of Various Reaction Parameters on SST.
e reaction parameters affecting the simultaneous saccharification and transglycosylation (SST) included solution pH, temperature, enzyme dosages of β-amylase, pullulanase, and transglucosidase, and reaction time.
For the experiment to study effect of solution pH on the SST, the reaction was conducted by varying of pH values between 4.0, 4.5, 5.0, and 5.5. Meanwhile, the reaction temperature was fixed at 50°C, and enzyme dosages of β-amylase, pullulanase, and transglucosidase were 3.0 U/g, 0.8 U/g, and 15.0 U/g, respectively, during 12 hours of reaction time.
Towards the experiment to investigate effect of reaction temperature on the SST, the reaction parameters were fixed, including the enzyme dosages of β-amylase, pullulanase, and transglucosidase of 3.0 U/g, 0.8 U/g, and 15.0 U/g, respectively, at the optimal solution pH value obtained from the aforementioned experiment. Meanwhile the reaction temperature was varied between 45, 50, 55, 60, 65, and 70°C. e experiment for studying effect of β-amylase dosage on the SST was carried out with varying of β-amylase dosages of 1 U/g, 2 U/g, 3 U/g, 4 U/g, and 5 U/g. e dosages of pullulanase and transglucosidase were fixed at 0.6 U/g and 15 U/g, respectively. For the experiment to study effect of pullulanase dosage, the pullulanase dosages were changed in range of 0.2 U/g, 0.4 U/g, 0.6 U/g, 0.8 U/g, and 1 U/g with simultaneously adding the optimal dosage of β-amylase obtained from aforementioned experiment and a transglucosidase dosage of 15 U/g. Finally, the experiment for investigation of effect of transglucosidase dosage was conducted by fixing both optimal dosages of β-amylase and pullulanase which were gained from above experiments with varying of transglucosidase dosages of 3 U/g, 5 U/g, 10 U/g, 15 U/g, 20 U/g, 25 U/g, and 30 U/g. All reactions conducted in the experiment to investigate effects of various enzymes on the STT were maintained at optimal pH value and temperature which were achieved from above experiments. At the end of reaction point, the enzymes were deactivated by boiling the mixture for 10 min. Next, all undesirable sugars contained in post-STT solution were removed using Saccharomyces cerevisiae var. diastaticus BE 134 according to procedure as described in Section 2.4.3. e posttreated mixture was then used to determine the IMO's constituents using HPLC-RID. e experiment to investigate effect of reaction time on the SST for synthesis of IMOs was performed by varying of reaction time from 6 h to 48 h. e other operational parameters including optimal solution pH, reaction temperature, and dosages of different enzymes which were obtained from the aforementioned experiments were fixed in this reaction.  Figure S1 (in Supplementary Materials). e details of the TLC method used in this study were adapted from [39]. A 1.0 μL sample and standard samples containing glucose, maltose, maltotriose, isomaltose (IMO2), and isomaltotriose (IMO3) were spotted on the silica gel 60 chromatoplates F254 (20 cm × 20 cm; Merck KGaA, Germany) by a pipette with a distance of 10 mm from bottom to top. e distance between the spotting points on the chromatoplates was 10 mm. e spotting points on the chromatoplates were then dried to maintain their sizes in range from 3 to 4 mm. Afterwards, the chromatoplates were impregnated into mixture of solvent consisting of n-butanol : isopropanol : water at ratio of 50 : 25 : 20 (v/v/ v) in a TLC developing tank. Herein, the solvents and oligosaccharides are moved upwards by capillary force. e difference in interaction force between solvents, oligosaccharides compositions, and thin layer is the principle to determine the oligosaccharides components in this sample.
All raw HPLC graphs of IMOs obtained at various experiment conditions are presented in Figures S2-S5

Effects of Various α-Amylase Enzyme Preparations on the Liquefaction Process.
e slurry of SPS with a ratio of 25% (w/v) was prepared and then used to synthesize IMOs by a series of enzymatic reactions, consisting of liquefaction and simultaneous saccharification and transglycosylation. e liquefaction reaction aims to choose α-amylase enzyme preparation for the highest contents of oligosaccharides components, in which the content of G 2 to G 6 is more noticeable than the remaining oligosaccharides because these substrates increased in the formation of maltose and maltotriose and could be also used directly by transglucosidase enzyme in the next step. us, increasing the content of G 2 to G 6 shortens the simultaneous saccharification and transglycosylation time required for maximum concentrations of IG 234 . e contents of oligosaccharide components in liquefaction process of SPS with adding different α-amylase enzyme preparations are presented in Table 1. e results in Table 1 show that using four different α-amylase enzyme preparations leads to formation of different liquefaction ingredients. Hydrolysis of Spezyme Xtra preparation gave that the total oligosaccharides contents from G 1 to G 10 (G 1 -G 10 ) and G 2 to G 6 (G 2 -G 6 ) were highest at 73.95% and 49.24%, respectively. However, preparations of Liquozyme SC DS, Termamyl SC DS, and Spezyme Alpha had contents of both G 1 -G 10 and G 2 -G 6 lower than those of Spezyme Xtra preparation. e contents of G 1 -G 10 and G 2 -G 6 were, respectively, 40.46-68.59% for Liquozyme SC DS, 34.32-65.70% for Spezyme Alpha, and 39.56-69.02% for Termamyl SC DS. From data in Table 1, as can be seen, the formed glucose content in liquefaction was relatively low, only from 0.0 to 1.68%. Also, the amount of formed starch residue was relatively small and there was significant difference between the enzyme preparations, from 1.56% to 2.15%. e detailed contents of each oligosaccharide in the hydrolyzed solution with α-amylase enzyme preparations are indicated in Figure 2. What stands out from data in Figure 2 is that content of G 6 in liquefied solution by Spezyme Xtra reached 20.89% which was higher compared with Liquozyme SC DS, Spezyme Alpha, and Termamyl SC DS. To be specific, the content of G 6 was 18.07 g/L, 15.79 g/L, and 17.02 g/L, respectively, for Liquozyme SC DS, Spezyme Alpha, and Termamyl SC DS. However, amounts of G 2 , G 3 , and G 4 possessed similar trend for all four applied enzyme preparations in liquefaction. Meanwhile, the concentration of G 5 formed when hydrolyzed by Spezyme Xtra (11.82 g/L) was higher than those of other preparations (5.85-7.86 g/L). Based on these detailed results, it is clear that hydrolysis properties of each enzyme preparation in liquefaction had distinct difference. e addition of Spezyme Xtra preparation to liquefaction reaction gave the highest concentration of oligosaccharides components without generation of a low amount of both free glucose and starch residue. Moreover, temperature for liquefaction reaction using Spezyme Xtra was 80°C which was lower than those of other preparations triggering energy saving for overall synthesis procedure of IMOs. In particular, the statistical analysis also shows that there was a significant difference between contents of oligosaccharides components, glucose, and starch residue when different enzyme preparations were supplemented into liquefaction reactions. erefore, the Spezyme Xtra was chosen as the best enzyme preparation for the liquefaction of SPS in the synthesis procedure of IMOs.

Effects of Solution pH and Temperature on SST for the Synthesis of IMOs.
e solution pH and temperature are two important reaction parameters affecting the simultaneous saccharification and transglycosylation (SST) by β-amylase, pullulanase, and α-transglucosidase for formation of IMOs from SPS. e results for investigation of effect of these parameters on the SST reaction for the synthesis of IMOs from SPS are illustrated in Figure 3.
During the SST reaction for synthesis of IMO, the enzymes of β-amylase and pullulanase were used to generate more maltose and other oligosaccharides which served as substrates for α-transglucosidase to produce IMOs. However, each enzyme exhibits a good activity at a certain pH and temperature. Namely, each kind of enzyme had an optimal pH and temperature value. In this study, the concentration of IMOs (IG 234 ) was calculated by the sum of IG 2 , IG 3 , and IG 4 concentrations. Panose was not observed in this study because its amount gradually decreased until the end of transglycosylation caused by hydrolyzation and did not produce an important amount by α-glucosidase (transglucosidase) [3,32]. Plot concentration of IG 234 versus solution pH values (Figure 3(a)) and various reaction temperatures (Figure 3(b)) showed that concentration of IMOs (IG 234 ) strongly depended on the pH and temperature.
According to the manufacturer's description (Megazyme), the barley β-amylase, pullulanase M2 from Bacillus licheniformis, and transglucosidase from Aspergillus niger exhibiting their maximum activities at pH and temperature values were 6.0 and 60°C, 4.5-5.5 and 55-60°C, and 4.5 and 70°C, respectively. Based on the data provided by manufacturer, the experiment to investigate effect of solution pH Values are mean ± standard deviation of duplicate analyses. Values followed by the same letter in the same column were not significantly different at p � 0.05. Journal of Food Quality in this study was performed with varying pH from 4.0 to 6.0, adding the barley β-amylase, pullulanase, and transglucosidase to SST reaction at dosages of 3 U/g, 0.6 U/g, and 15 U/g, respectively, at 55°C for 12 h of reaction time. It can be seen from Figure 3(a) that there was an increase in concentration of IG 234 when pH increased from 4.0 to 5.0. e maximum IG 234 concentration was 58.39 g/L at pH of 5.0, while pH of 4.0 for concentration of IG 234 was the lowest. When pH was further increased to 5.5 and 6.0, the concentration of IG 234 saw a slight downward trend. From these results, the most suitable pH value range for activation of all three enzymes that led to maximum concentration of IG 234 was between 5.0 and 6.0. Also, the optimal pH value for SSTreaction was chosen as 5.0 and this pH would be used for next experiments. Figure 3(b) shows the effect of reaction temperatures on the formation of IG 234 concentration from SPS by SST reaction. e concentration of IG 234 witnessed an increase when the reaction temperatures were increased from 40 to 55°C. e concentration of IG 234 maximized at 59.12 g/L at 55°C. At 50°C, the concentration of IG 234 was 57.5 g/L. e statistical analysis shows that there was no significant difference in obtained concentration of IG 234 in the two cases. Besides, due to economic aspect, the reaction temperature of 50°C was chosen as optimal value for SST reaction. Moreover, the data in Figure 3(b) also indicate that there was a considerable decrease in concentration of IG 234 when the reaction temperatures were further risen. To be specific, the concentrations of IG 234 were 40.82, 29.74, and 37.16 g/L, respectively, at 60°C, 65°C, and 70°C. e results of this study clearly showed that range of optimal pH and reaction temperature had good agreement with the manufacturer's instructions. Besides, the findings were similar to results reported by Niu et al. [4]. Specifically, the study showed that all three enzymes of β-amylase, pullulanase, and transglucosidase obtained from similar biological sources exhibited over 50% of their maximum activity at pH from 5.0 to 6.0 and reaction temperature from 45 to 65°C.

Effects of Dosages of Various Enzymes on SST for the Synthesis of IMOs.
As is known, the barley β-amylase only released sequentially maltose from the nonreducing ends of the starch polymers [9]. To determine effect of dosage of β-amylase on the synthesis of IMOs, different dosages of β-amylase were added to the SST reaction. Meanwhile pullulanase and transglucosidase were used in all experiments at dosages of 0.6 U/g and 15 U/g, respectively, at pH of 5.0 and reaction time of 12 h. e achieved data are demonstrated in Table 2.
What stands out from data in Table 2 is that when the dosage of β-amylase increased from 1.0 U/g to 3.0 U/g, the IG 234 concentration increased from 58.66 to 63.65 g/L, respectively. e highest IG 234 concentration reached 63.65 g/l at β-amylase dosage of 3.0 U/g. However, a further increase in β-amylase dosages led to a drop in the concentrations of IG 234 . To be specific, the concentration of IG 234 achieved 62.76 g/L and 57.09 g/L at β-amylase dosages of 4.0 and 5.0 U/g, respectively. e results showed that there existed an optimal dosage of β-amylase in SST reaction for production of IMOs from SPS. ese results can be explained as follows: when the dosage of β-amylase was increased to a certain level, the formation of IMOs was increased because the enzyme β-amylase enhanced production of more maltose and maltotriose in the SSTreaction mixture. is led to enhancement of production of IMOs by transglucosidase [9]. Moreover, the presence of β-amylase in SSTreaction also catalyzed for hydrolysis of maltodextrin to form maltooligosaccharide, an essential substrate for synthesis of IMOs [25].
Besides, the statistical analysis indicates that there was a good correlation between the concentrations of IG 234 when adding β-amylase dosages of 2.0 U/g, 3.0 U/g, and 4.0 U/g. Nevertheless, there was a better correlation in case of using β-amylase dosage of 2.0 U/g compared with 1.0 U/g. Similarly, the β-amylase dosage of 4.0 U/g gave closer correlation than those of cases of 2.0 U/g and 3.0 U/g of applied β-amylase dosages. Derived from the results and discussed as aforementioned, the β-amylase dosage of 3.0 U/g was chosen as the optimal value for the SST reaction to synthesize the IMOs from SPS. In this study, the pullulanase enzyme was also selected to be added to SST reaction for synthesis of IMOs from SPS. e pullulanase is an enzyme that specifically hydrolyzes α-1,6-linkages in starch molecules [40]. Pullulanase has proved the remarkably increase of the IMOs yield which had no harmful effects on IMOs quality [4].
is can be explained as follows: the pullulanase requires each of the two chains linked by an α-1,6-glycosidic bond containing at least two α-1,4-linked glucose units. us, the smallest substrate for pullulanase is the tetrasaccharide 6 2 -α-maltosylmaltose [28]. Meanwhile, the main products formed due to the transglucosidase enzyme activity have no such structural form. To determine effect of adding the pullulanase on production of IMOs by SST reaction from SPS, the different amounts of pullulanase M2 were supplemented into the reaction. e dosages of both β-amylase and transglucosidase were 3.0 U/g and 15.0 U/g, respectively, which were simultaneously added to the SST reaction. e solution pH and reaction time were maintained at 5 and 12 h, respectively. e obtained results are shown in Table 2. As can be seen from data in Table 2, the concentration of IG 234 saw a significant increase when the pullulanase dosage was increased from 0.2 U/g to 0.8 U/g and peaked at 0.8 U/g with 69.24 g/L of IG 234 . However, a further growth in pullulanase dosage led to a slight decrease in the concentration of IG 234 with 67.2 g/L at 1.0 U/g of pullulanase dosage. ese results were because the pullulanase enhanced hydrolysis of α-1,6linkages in starch molecules [28]. In particular, the combination between pullulanase and β-amylase at a suitable ratio in the same SST reaction condition proved the promoting generation of more short chain maltose and maltodextrin which resulted in improving the synthesis performance of IMOs [1,7]. Besides, the HPLC analysis data also showed that application of the pullulanase for treatment of IMOs product had no negligible effects on the quality of IMOs. In particular, the pullulanase did not mostly hydrolyze α-1,6-glycosidic linkings in the product mixtures [4]. Moreover, statistical analysis also showed that the IG 234 concentrations in the cases of 0.8 U/g and 1.0 U/g had similarities.
us, the pullulanase content of 0.8 U/g was selected for the next experiments.
Together with β-amylase and pullulanase, transglucosidase was also added to the SST reaction of SPS for synthesis of IMOs. e effect of various dosages of transglucosidase on the IMOs formation was examined, and results are summarized in Table 2. e IG 234 concentrations gradually increased from 60.84 g/L to 67.37 g/L when the transglucosidase dosages were increased from 3.0 U/g to 10.0 U/g. Meanwhile, the concentration of IG 234 witnessed a gradual drop trend when the dosage of transglucosidase went to 30 U/g. To be specific, the IG 234 concentration decreased to 53.47 g/L at transglucosidase dosage of 30 U/g. is result can be explained based on transglucosidase properties. Because the transglucosidase can simultaneously hydrolyze both α-1,4-glycosidic and α-1,6-glycosidic linkings. However, the hydrolysis rate of α-1,4-glycosidic linkings was more rapid than that of α-1,6-glycosidic bonds by about twice [9]. As a result, when the transglucosidase concentration is high, the hydrolysis rate of oligosaccharide substrates is promoted, which leads to release of more undesirable free glucose molecules [32]. It causes a decrease in IMOs concentration. Furthermore, the formed IMOs products can also be hydrolyzed at high transglucosidase dosage in the SST reaction. Also, statistical analysis indicated that the transglucosidase enzyme dosages of 10.0 U/g and 15.0 U/g gave the same IG 234 concentration. us, derived from the economic aspect, the transglucosidase dosage of 10.0 U/g was chosen for the following experiments.
In summary, the experiment to investigate effect of dosages of various enzymes on simultaneous saccharification and transglycosylation for synthesis of IMOs chose the optimal values of enzymes dosages for SST reaction to produce IMOs from SPS. ese optimal dosages were 3.0 U/ g, 0.8 U/g, and 10.0 U/g, respectively, for β-amylase, pullulanase, and transglucosidase.

Effects of Reaction Time on SST for the Synthesis of IMOs.
e experiment to study effect of reaction time on the liquefied liquid was conducted at pH of 5.0, reaction temperature of 50°C, and dosages of β-amylase of 3.0 U/g, pullulanase of 0.8 U/g, and transglucosidase of 10 U/g with varying of reaction time between 6 h and 48 h. e experimental results are highlighted in Figure 4.
What stands out from data in Figure 4 is that there was a marginal increase in the concentration of individual IGs (IG 2 -IG 4 ) with an increase in the reaction time from 6 h to 48 h. Also, total concentration of IG 234 saw a corresponding increase. Specifically, the IG 234 concentration was increased from 60.61 g/L to 68.85 g/L with an increase in reaction time from 6 h to 12 h, respectively. is trend had no negligible change at the reaction time between 12 h and 48 h. is was due to the ability of transglucosidase enzyme in directly using the formed IMOs as substrates to hydrolyze them into other by-products [4]. As mentioned above, the transglucosidase can hydrolyze both the α-1,4-glycosidic and α-1,6-glycosidic bonds and the hydrolysis reaction rate of α-1,4-glycosidic bond is quicker than hydrolysis reaction rate of glycosidic α-1,6-bond by twice [9]. erefore, the SST reaction required the optimal time period to reach a maximum synthesis performance of IMOs from SPS. Besides, the number of α-1,4-glycosidic bonds in the hydrolyzed liquid saw a downward trend, while the number of α-1,6-glycosidic bonds was gradually increased; and when the number of α-1,4-glycosidic bonds was decreased to a low level and the α-1,6-glycosidic linkings were increased to an equilibrium level, the IG 234 content only increased slightly followed by a decrease trend in the IG 234 content. In this study, the concentration of IG 234 formed after 12 h was 68.85 g/L, which was not much lower than that of 24 h with an IG 234 concentration of 71.58 g/L. Moreover, the contents of IG 3 and IG 4 tended to decrease with an increase in reaction time from 24 h to 48 h. Besides, both IG 3 and IG 4 are considered as difficult or undigested IMOs by the human's enzyme system [9]. e statistical analysis also indicates that there was a significant difference in the concentration of IG 234 formed after 12 h reaction time compared to other cases. At reaction time of 24 h, 36 h, and 48 h, the contents of formed IG 234 had no significant difference at p � 0.05. erefore, the most suitable time for the SST reaction to synthesize IMOs from the SPS was 12 h. Comparing the results obtained in this study about the gained IMOs concentrations with other studies which used conventional methods for production of IMOs showed that IMOs concentrations obtained in recent work were higher those of other studies at the same reaction time period. To be specific, the maximum IMOs concentration that was obtained from liquefaction of the banana starch by Termamyl SC, barley β-amylase, and transglucosidase L enzyme in saccharification and transglycosylation reactions was 70.74 ± 4.09 g/l after 24 h of saccharification and 12 hours of transglycosylation [9]. It is clear that total reaction time for two separate steps of saccharification and transglycosylation was 36 h, which was much higher than that of this study.
us, the simultaneous combination of β-amylase, pullulanase, and transglucosidase activities in simultaneous saccharification and transglycosylation reaction of SPS for synthesis of IMOs shortens reaction time by three times compared with other studies with the same concentration of IMOs.

Conclusions
In this study, a two-step synthesis procedure of IMOs from sweet potato starch was successfully developed. Investigation of factors affecting the liquefaction and simultaneous saccharification and transglycosylation reactions chose the most suitable conditions for production of IMOs from inexpensive and abundant SPS source. In the liquefaction, the Spezyme Xtra enzyme preparation gave the highest content of oligosaccharides components. e optimal conditions for liquefaction reaction of SPS to synthesize IMOs were at SPS slurry of 25%, α-amylase dosage of 1.0 CU/g, and pH of 5.8 at 80°C for 30 min of reaction time. At this condition, the compositions of oligosaccharides reached 73.95 ± 0.02% (w/w) for G 1 -G 10 and 49.24 ± 3.19% (w/w) for G 1 -G 6 . In the simultaneous saccharification and transglycosylation reaction, the highest concentration of IMOs was achieved as 68.85 ± 1.82 g/L at optimal operational conditions, including pH of 5.0, β-amylase dosage of 3.0 U/g, pullulanase dosage of 0.8 U/g, and transglucosidase dosage of 10.0 U/g at 50°C for 12 h reaction time. In particular, the synthesis procedure of IMOs applied simultaneous saccharification and transglycosylation shortens the reaction time of SST step for synthesis of IMOs from SPS by three times. e purification of raw IMOs using Saccharomyces cerevisiae var. diastaticus BE 134 was also improved. In conclusion, the synthesis procedure of IMOs derived from SPS sources was feasible to apply in practice to produce useful prebiotics. In our next studies, it is necessary to further investigate the IMOs' structure in the finished products and the application of supplement of IMOs into food products also needs further study.

Data Availability
e data used to support the findings of this study are included within the article and supplementary materials.

Additional Points
(i) Isomaltooligosaccharide (IMO) was synthesized from low-cost sweet potato starch (SPS). (ii) e optimal condition for the SST reaction of SPS was systematically studied. (iii) e SST reaction of SPS to synthesize IMOs shortens reaction time by three times. (iv) e yeast cells were applied to purify IMO products from SPS.

Conflicts of Interest
e authors declare no conflicts of interest.

Supplementary Materials
Supplemental Figure S1: