Quantification of Oil Fractions of Deep-Fried Wheat Dough and Batter Enriched with Oat and Wheat Bran

This study investigated oil distribution in two types of deep-fried dough products with two moisture levels (65 and 100 wt. %), two bran types (oat and wheat bran), and five bran concentrations (5–20%). The total oil content of fried products was categorised as surface oil (SO), penetrated surface oil (PSO), and structural oil (STO) using a spectrophotometric method. Moisture loss reduced (
 
 p
 <
 0.05
 
 ) from 23.35% in the control to 15.19% fried batter (FB) and to 20% oat bran (OB), while a reverse trend was observed in the fried dough. Reduction of total oil from 0.43 g/g in the control to 0.38 g/g at 20% OB and 8% wheat bran (WB) was observed. At 15% OB and 20% WB, total fat reduced from 0.41 g/g in the control to 0.26 g/g of fried dough (FD). The trend STO < SO < PSO was observed in FD, while FB followed a SO < STO < PSO trend. This investigation indicated that oil uptake reduction in fried dough products is achievable through ingredient modifications. The method of oil distribution measurement used herein can be applied to other thick deep-fried food systems in the assessment of product quality.


Introduction
Fried foods are popular worldwide because of their palatability owing to the flavour imparted by fats and oils in foods. Deep frying is a popular heat and mass transfer unit operation which entails immersion of food in hot oil at high temperatures (160-200°C) under atmospheric conditions, thus yielding processed foods with unique textures and rich flavours [1]. e transfer of heat from oil to food gives rise to various chemical reactions which cause cellular and subcellular changes such as starch gelatinisation, crust formation, protein denaturation, moisture loss, oil uptake, and colour and flavour development. Mass transfer is explained as the evaporation of moisture from food during frying, which creates crevices or pathways in food for oil uptake first in the crust and then gradually to the crumb [2]. is makes oil uptake reduction, modelling, and distribution important quality parameters in fried foods. e reduction of the lipid content in fried foods is required mainly because it positively correlates with diabesity and coronary diseases [3].
Based on different oil absorption mechanisms, oil fractions in fried foods can be categorised based on their location: surface, penetrated surface, and structural oil. Surface oil (SO), which represents the oil fraction that remains on the food surface after removal from the frying medium, is governed by the relationship between capillary forces and interfacial tension which causes the adsorption of oil to food [4,5]. Structural oil is absorbed into the core of food through crevices caused by a continuous replacement of moisture by fat during frying [6]. Penetrated surface oil (PSO), located in the crust of food, is oil suctioned into food at the cooling stage through a vacuum effect caused by the condensation of steam [7,8]. PSO is regarded as the most important of the three fractions because it contributes the most to the total oil uptake and is greatly influenced by the structural integrity, surface roughness, and permeability of the food crust [2]. An integral constituent of wheat flour dough is water, needed for hydration and activation of the gluten network; to achieve this, the right amount of water is needed [9]. Moreover, water determines the texture and volume expansion of the product because of water vapour which is unable to escape from the food core into the surrounding medium. As regards frying, one of the known mechanisms is a water-oil replacement, which states that the amount of oil lost is directly proportional to the initial water content of the fried product [10]. However, the process of moisture loss and oil uptake is asynchronous given that moisture loss occurs during the frying process and oil uptake during the cooling period [1].
Wheat bran (WB) and oat bran (OB) are low-cost fibre sources for food product development. eir benefits, attributed to insoluble and soluble fibre contents and nutrient load, include calorific reduction of foods, weight modulation, improvement of gut health, increased intestinal transit time [11,12], and oil uptake reduction in fried dough [13][14][15][16]. e effect of bran inclusion on oil uptake reduction in fried dough foods is associated with ingredient formulations based on the following mechanisms: (a) increased viscosity of the food matrix [17], (b) water retention augmentation [13], and (c) barrierforming properties [14,18]. Oil reduction effects of WB in fried foods are limited to ≤20% substitution, while those of OB are up to 11%. At higher amounts, WB increased oil absorption due to the dilution of the gluten network by coarse WB. Hence, it is recommended to reduce the particle size of bran to dimensions close to flour particles [19]. In this study, WB and OB were pulverised to 200 µm size. e product investigated in this paper is called magwinya/vetkoek, a deep-fried dough common to the Southern Africa region, and it is similar to Belgian oliebollen. In recent studies, oil uptake reduction attempts on magwinya were conducted using mixtures of wheat flour and oat bran, psyllium husk fibre [20], guar gum (unpublished), and WB of varying particle sizes [16,21]. Most studies on oil distribution in fried foods focused on potato products [7,8,22] and one study on surface oil of the fried wheat dough model [23]. To the best of our knowledge, this present work is the first study on oil distribution in these fried products (magwinya). erefore, the objective of this study was to quantify the oil fractions (SO, PSO, and STO) in cereal fried snacks with different moisture levels and cereal bran (OB and WB).

Sample Preparation
2.1.1. Materials. Jungle oat bran (OB), wheat bran (WB), and wheat flour (SASKO, South Africa) were sourced from local grocery shops in South Africa. Sudan III (A18318, Alfa Aesar, England) and petroleum ether (40°C-60°C) were of analytical grade. Coarse WB and OB were milled using an ultracentrifugal mill (Retsch ZM 200, Haan, Germany) fitted with a 200 µm sieve. Milled bran was transferred into air-tight polyethylene bags and stored at 3°C until further use. Bran was partially substituted with wheat flour at 5, 10, 15, and 20% to produce the fried products.

Production of Deep-Fried Products.
e method described by Kwinda et al. [20] was used for the preparation of fried batter and dough with slight modifications. Dry ingredients (bran-wheat flour, sugar-15 g, salt-1 g, and yeast-1 g) were weighed, and water (65-100 ml) was added until the ingredients are homogeneously mixed to form a dough and batter, respectively ( Figure 1). e dough was kneaded in a mixer (Russell Hobbs RHSB237, South Africa) for 10 min and cut into 50 g mass and formed into a ball. e dough and batter were fermented at 30°C and fried at 180°C for a total of 5 min (dyed and nondyed oil) in a deep fryer (Russell Hobbs RDF300, South Africa) with an in-built automatic temperature and time control system. Oil was preheated for 1 h before frying commenced and discarded after 3 h of frying to minimise thermal degradation of oil. For each experimental run, four replicates of batter and dough were deep-fried.

Preparation of the Indicator Oil Bath Dyed with Sudan
III. Approximately 1 g Sudan III (A18318, Alfa Aesar, England) was weighed into a volumetric flask. About 100 ml sunflower oil was added and agitated to partially dissolve the dye and thereafter made up to 1 L mark. e volumetric flask containing the dye solution was transferred to a heating stirrer plate at 30°C for 24 h until complete dissolution. A dyed oil bath was prepared by adding 233 ml of indicator oil to 3.5 L oil [8].

Frying Process.
Frying was conducted in two stages according to the method of Ouchon et al. [8] with slight modifications: (i) samples were fried in plain (nondyed) oil for 4 min 40 sec before transfer to dyed oil for the remaining 20 sec. Samples were removed from the fryer, drained, cooled for 30 min, and weighed [7,8]. Four samples were fried for each experimental run. e dough-to-oil ratio was kept at 1 : 23 by volume to avoid drastic fluctuations in frying temperature [24]. Temperature gradients in the oil bath were maintained by constantly stirring while flipping the samples every 30 sec. Frying time and temperature of 5 min and 180°C were chosen as the optimum frying time and temperature as predetermined from preliminary experiments. e choice of 20 sec in dyed oil was made for three reasons: (i) this study was designed following the method of Ouchon et al. [8] where potato cylinders were introduced to dyed oil at about 20 sec to the end of frying. (ii) We assumed that 20 sec will have the most significant effect based on the study of Ufheil and Escher [7] where the product was interchangeably fried in plain and dyed oil from 0 sec to 120 sec. (iii) At a total of 5 min (300 sec) frying time, the ratio of time spent in plain versus dyed oil in our study was 14 : 1 (sec) which is low compared to the time ratio in the study of Ufheil and Escher [7] and similar to the time in the study of Ouchon et al. [8]. It is on the above premises that 20 sec was chosen. Since the purpose of this study was to quantify various oil fractions in magwinya, we did not focus on the optimisation of time in plain and dyed oil during frying. We, however, recommend further studies on time variation in the frying of our products (plain versus dyed oil).

Standard Curve for the Determination of Sudan III Concentration in Frying Oil.
Solutions of different concentrations (0.2, 0.4, 0.6, 0.8, 1.0, and 1.2 mg/ml) of Sudan III (A18318 Alfa Aesar, England) in sunflower oil were prepared as explained earlier. Each solution was diluted at 1 : 10 with petroleum ether (40°C to 60°C) (Sigma-Aldrich), and the absorbance was measured at 509.6 nm with a spectrophotometer (Biowave II, Biochrom Ltd., Cambridge, England) at ambient temperature. e standard curve of concentration versus absorbance was obtained.

Weight of Fried Products.
Two sets of weights were collected using a digital weighing balance: (i) fresh weight of cooled samples and (ii) weight after washing with petroleum ether-this was used in the calculation of surface oil (SO).

Moisture Loss Determination.
Moisture content was determined using the approved AACC method 44-15 [25]. Magwinya samples were thinly sliced into a preweighed moisture dish and dried in an OTE80 forced-air oven (Prolab Instruments, Reinach, Switzerland) at 105°C for 24 h. Dried samples were transferred to a desiccator to cool. Moisture contents of dough and batter were determined, and moisture loss was calculated using the following equation Draining and cooling to ambient temperature Dry ingredients (flour -100 g, yeast -1 g, salt -1 g, sugar-15 g) 100 ml water added to dry ingredient, mixed with a spatula to form a batter 65 ml water added to dry ingredient, in a mixer for 10 min to form a dough Batter scooped directly into hot oil Dough formed into round shape before frying where W i is the weight of oil (g) in the beaker and W m is the weight of the sample after washing with ether.

Penetrated Surface Oil and Structural
Oil. After washing with petroleum ether, the samples were finely cut and dried for 24 h at 60°C. Subsequently, the dried samples were pulverised in a grinder, and 5 g of the ground sample was transferred into 25 × 80 × 1.5 mm cellulose extraction thimbles (Whatman Intl. Ltd., Maidstone, UK). Fat extraction was conducted using an automated Soxhlet machine based on AACC method 30-25.01 [25]. Soxhlet-extracted oil was diluted with petroleum ether at ratio 1 : 10, and the absorbance was measured at 509.6 nm using a spectrophotometer (Biowave II, Biochrom Ltd., Cambridge, England) against the standard curve obtained from the standards. PSO was calculated using equation (3). Dyed oil corresponds to oil absorbed at the end of the frying process and has penetrated the structure of the product during cooling, that is, PSO. e structural oil and total oil contents were calculated from equations (4) and (5) [8].
2.6. Statistical Analysis. Frying experiments and analyses were conducted in four replicates. Multivariate linear regression was used to test the main and interaction effect of factors (amount of bran and water addition) on the dependent variables. Analysis of variance using Duncan's multiple range test was used to separate means (p < 0.05). Association between responses was determined using Pearson's correlation test [16].

Moisture Loss of Cereal Fried Products.
Moisture loss corresponds to the amount of water lost due to frying, while moisture content is the available moisture in food which can be expressed on a wet or dry basis [27]. In general, the moisture values for fried batter were higher than those for fried dough primarily because of the lower initial water content in the dough formulation. Moisture loss of oat bran fried batter (OBFB) samples ranged significantly (p < 0.05) from 15.19 to 22.34% with the highest value in OB20. ere was no significant change in the moisture loss in OB5 and OB10; nonetheless, a significant increase was observed in OB15 and OB20 (Figure 2(a)). Control had the least amount of moisture retained in food at the end of the frying process. OB significantly enabled moisture retention in food which is a characteristic favoured by magwinya consumers. Interestingly, a different trend was observed for oat bran fried dough (OBFD) in terms of moisture retention and loss. Mean moisture loss ranged from 14.21 to 17.66% in OBFD. e control sample was significantly lower, and OBD20 was markedly higher (p < 0.05) than the rest of the samples. In terms of moisture retained in the product, control had the highest moisture content of 34.54%, and it was not significantly different from the sample with 10% OB. Moisture loss in wheat bran fried batter (WBFB) samples ranged from 18.69 to 23.36%, followed a significant (p < 0.05) upward trend from 5 to 20% WB addition (Figure 2(b)), and had no difference, meaning that WB had a marked negative effect on moisture loss of fried samples. is may be attributed to the increased water holding capacity of WB because of more binding sites in the smaller particle size [28]. Moisture loss in wheat bran fried dough (WBFD) samples ranged from 11.79 to 14.21%, and a downward significant trend (p < 0.05) was observed in moisture loss. is implies that WB allows the retention of moisture in a low-water dough system. Water is an important ingredient in a dough matrix because it is needed for the hydration of starch molecules, thereby impacting the texture of the final product. Besides, water influences the volume expansion of the fried product through the process of water vaporisation, crust pore formation, and structural integrity of the fried product [29]. In a dough system, free water is lost first from the product in the first few seconds of frying. e variation in the moisture losses reported in this study can be explained by the interaction between the binding sites on bran and water molecules [19], which reduced the amount of free water lost during deep frying.

Surface Oil Content of Fried Products. Surface oil (SO) is
oil that adheres to the food surface after removal from frying oil [8], and it is equally referred to as adhered oil [23]. e amount of oil absorbed into food during the cooling stage is largely dependent on oil present on the food surface after the removal of frying oil.
e SO values of WB samples are presented in Figure 3(a). SO of WBFB with 5 and 20% showed a significant increase (p < 0.05) from the control, and other samples showed no difference. Similarly, in WBFD (Figure 3(b)) samples, all samples showed a significant decrease (p < 0.05) from the control, except WB10. ere was no marked difference (p < 0.05) between SO of control and OB samples; however, there were differences amongst the samples (Figure 3(c)). However, the inclusion of OB in fried dough significantly reduced (p < 0.05) SO compared to the control (Figure 3(d)). Generally, fried dough showed higher SO than fried batter. Additionally, SO was higher in WB samples than in OB samples. is may be because of the insoluble nature of WB fibres present which reduced moisture retention and caused rapid crust formation, thus slowing down the movement of oil into the product. Multivariate linear regression revealed that bran addition, product formulation, and interaction effect of both factors all had a significant effect (p < 0.05) on surface oil of the products. ese observations are supported by a previous study on microstructural analysis of fried products [30]. e amount of oil absorbed into food after frying is highly dependent on surface oil. is is because the rigorous escape of steam from the food surface leads to the formation of crust which is characterised by tiny crevices that can allow the permeation of oil into the food core. During cooling, as the pressure gradient in the core of food drops, surface oil is absorbed through capillary forces into food through cracks in the crust [10]. erefore, it is recommended that an appropriate draining technique be applied to remove surface oil, thus reducing oil uptake.
An increase in the SO content of fried batter was reported by anatuksorn et al. [23]. Contrary to this, we observed a higher SO content in the fried dough samples.
is contradiction in the results could be due to (i) the difference in the surface oil collection method, where anatuksorn et al. [23] used the dipping method, and our product surface was washed to collect adhered oil. Dipping the sample could have caused the extraction of subsurface oil alongside adhered oil. (ii) Furthermore, a small sample size (<10 g) coupled with a prolonged frying time of 7 min increased crust-crumb ratio which meant more surface oil. From this study, it was observed that SO of fried dough was higher than fried batter. is can be explained as follows: (i) the dough had better structural integrity due to good pliability during kneading which led to gluten formation and gas retention, and (ii) the consistency of the batter caused more bubble formation which made the fried batter spongier (than its dough counterpart) and, as a result, could not retain oil on the food surface.

Penetrated Surface Oil of Fried Products.
Penetrated surface oil (PSO) was significantly higher in fried batter samples. PSO was in the range 0.19-0.29 g/g and 0.08-0.22 g/ g in WBFB and WBFD, respectively (Figure 3), while OB samples ranged from 0.19 to 0.30 g/g and 0.12 to 0.22 g/g in OBFB (Figure 3(c)) and OBFD, respectively (Figure 3(d)). A significant decrease (p < 0.05) in PSO was observed in WB5 and WB20, while in fried dough, a reduction was only reported at WB10 and WB20 concentrations. For OBFB samples, a significant reduction from the control was found at OB10 and OB20 concentrations, while in OBFD samples, the reduction was seen as 15%. Linear and interaction effects of the formulation and bran addition had a significant effect (p < 0.05) on PSO of magwinya. PSO is considered the most significant oil fraction because it accounts for a higher percentage of the total oil content of fried foods [8,31]. PSO is the amount of oil absorbed in the crust microstructure, and this occurs toward the end of the frying (10-20 sec) and cooling stage due to the change in temperature and core pressure gradient [31,32]. Dehydration in fried cereal snack (magwinya) is most intense at the surface because of the high temperature (180°C), which in turn leads to the formation of pores in the crust microstructure which are subsequently filled with oil and/or air as the core temperature of the product decreases. Oil uptake in fried foods is a surface-related phenomenon that starts at the onset of cooling due to the competition between drainage and suction forces through which oil moves into the crust substructure. In the current study, the percentage of PSO was highest in all samples as confirmed in previous studies [7,8,31]. Another factor that could potentially contribute to increased PSO is the food surface roughness, increasing the cracks in the crust which increases the uptake of surface oil [23,33].

Structural Oil of Fried Products. Structural oil (STO)
corresponds to the amount of oil that is embedded in the core of food. STO was in the range 0.12-0.15 g/g in WBFB and 0.04-0.11 g/g in WBFD, while STO for OBFB ranged from 0.04 to 0.17 g/g, and STO for OBFD ranged from 0.04 to 0.09 g/g (Figure 3). While the values of WB fried batter were close, those of OBFB were spread over a wide range, which could be attributed to the batter mass which was not as precise as the dough mass before frying. ere was no significant difference (p < 0.05) among WBFB samples, while STO showed a significant increase in WBFD samples at 5-15% WB concentration and a decrease at WB20. Control STO of OBFB decreased from 0.11 g/g to 0.04 g/g in OB15. Generally, STO values were significantly higher in fried batter than in fried dough samples. is may be a function of the higher porosity in the fried batter which allows the free flow of oil into the food core [30]. STO was the lowest of the three fractions. e case is contrary in the potato products in which SO was the lowest oil fraction [8].
is reveals that oil distribution in fried foods differs from one product to the other. Unlike thin products such as potato chips that absorb most of oil during frying, magwinya is high-moisture and thick food, and thus, less oil is absorbed during frying and more during cooling [31,32].

Total Oil Content of Cereal Fried Products.
e sum of all oil fractions SO, PSO, and STO makes up the total oil content of the fried products. e total oil content of the samples was in the range 0.38-0.50 g/g and 0.25-0.41 g/g in WBFB and WBFD (Figure 3). Oil uptake reduction in WBFB was at 20% WB (Figure 3(a)), while in fried dough, a significant decrease (p < 0.05) at 10-20% WB was observed (Figure 3(b)). e total oil content of OBFB samples was in the range 0.37-0.43 g/g with a significant reduction in OB10 and OB20 and 0.26-0.41 g/g in OBFD with consistent reduction with increments in OB concentration. Oil uptake in fried batter was higher than fried dough for both oat and wheat bran samples. is effect can be linked to the difference in initial moisture contents of both products. Regression analysis showed that the linear effect of the initial water content, bran addition, and interaction effect of both factors had a significant effect (p < 0.001) in wheat and oat bran samples. In the fried batter samples, there was a significant oil uptake reduction in OB samples, while WB samples showed less variation in the values reported. In the fried batter, an initial increase (OB5) in total oil uptake followed by a decrease was observed, while in OBFD, there was a steady increase from the control to OB15. A decrease in oil uptake from 0.43 (g/g db) in the control to 0.38 (g/g db) at 20 g OB inclusion in the fried dough was observed.
Yadav and Rajan [13] attributed the effect of oat fibre on oil uptake reduction in poori (an Indian fried flatbread) as a function of its significant water retention capacity which impeded moisture loss. Furthermore, the chemical composition of the fibre is less responsible for its oil binding capacity than the porosity of the fibre that serves as a storage space for water molecules when the fibre is hydrated, thereby reducing the oil binding property. Significant oil reduction effects of wheat and oat bran were observed in poori (an Indian deep-fried dough) at 3 and 11%, respectively. Moisture loss-oil uptake effect in fried products confirmed that higher initial water content led to increased oil uptake due to the formation of pores by moisture evaporation which in turn creates pathways for oil uptake. e higher the moisture loss, the higher the oil uptake. Moisture loss-oil uptake effect in fried products as adequately reviewed by Ziaiifar et al. [10] revealed that the outflow of water from food leaves cracks for oil to occupy.
Factors that influence this mass transfer phenomenon in succession are (1) the initial water content of food, which in turn determines (2) the rate of water vaporisation over the frying period which directly impacts (3) crust thermal conductivity and (4) crust texture and porosity. In terms of the effect of the initial water content, Gazmuri and Bouchon [1] reported no significant difference in oil uptake of the fried dough matrix with 38 and 44% of water content; nonetheless, they found a relevant difference when the amount of gluten in the products was considered. is "no significant effect may be caused by the small difference (6%) in the water content of the compared dough matrices." In this study, a wide difference (35%) in water contents of dough and batter could account for the higher oil uptake in fried batter samples. Hence, to control the crust and crumb texture of fried foods, the water retention capacity of the dough, initial water content, and moisture content after frying are important.
Frying is a heat and mass transfer phenomenon. Heat transferred from oil to the food culminates in mass transfer-moisture evaporation and oil influx into food. Volume expansion and heat transfer occur through a combination of convective (from oil to food) and conductive (within food) forces. e rate of heat transfer during frying is affected by bubble movement that occurs and may reduce when there is maximum or vigorous water loss which causes a resistance to heat transfer [10,34]. In this current study, fried batter had higher bubble movement than fried dough, which may be attributed to the high moisture content of fried batter and the high thermal conductivity coefficient of fried dough as higher heat transfer coefficients have been reported in the absence of vapour bubbling. Upon immersion in hot oil, the fried batter sank to the bottom of oil and floated under 10 sec, whereas the fried dough floated almost instantaneously. is variation in floatation time can be linked to water differences in the formulation and density of the products [35]. As water is lost during frying, the product volume increases, and the density reduces, which makes the fried products float. Floatation results from heat conversion of water to steam which causes a pressure that pushes against the food crust, and in turn, volume expansion puffiness occurs which causes food to rise to the surface of oil. Floatation may, as well, contribute to mass transfer differences in the products, thereby resulting in thermal conductivity variation at the top and bottom crust of the product. To combat this variation, the product was constantly turned every thirty seconds during frying.

Correlation of Oil Fractions, Weight, and Moisture Loss.
Pearson's correlation examined relationships among the properties of fried snacks as presented in Table 1. In OB fried batter, a negative correlation was reported between weight and moisture loss, meaning that products with higher weight values did not lose much moisture. ere was a negative significant correlation (p < 0.01) between PSO and STO, implying that higher PSO led to lower STO, and vice versa. Similarly, SO correlated positively with total oil uptake. is buttresses the point that oil absorbed into the sample during cooling depends on oil on the product surface. ere was no significant correlation between weight and oil fractions because the weights of fried dough were uniform. Moisture loss correlated negatively with various oil fractions except STO in fried dough samples (Table 1).
Although moisture loss has been reported to play a role in oil absorption, the negative correlation in this study may be explained by the effect of bran addition which impacts moisture loss and, in turn, oil content. Surface oil positively influenced PSO and total oil content, while PSO negatively correlated with STO, meaning that the higher the PSO, the lower the STO, and vice versa. is implies that these oil fractions are not uniformly distributed in fried cereal snacks (magwinya). Weight of WB fried batter correlated positively with moisture loss and PSO. e three oil fractions all correlated positively (p < 0.01) with the total oil content ( Table 2). In fried dough, weight correlated positively (p < 0.01) to STO and total oil content. Moisture loss correlated positively to PSO. Structural oil correlated positively with PSO, implying that an increase in one led to an increase in the other. e total oil content had a positive significant relationship with weight, PSO, and STO.

Conclusions
e inclusion of oat bran (OB) in fried dough from 5 to 20% and fried batter at 10% reduced the total oil content. Significant oil uptake reduction in wheat bran (WB) fried dough (WBD) occurred at 10-20%. Oil distribution in fried dough products (magwinya) revealed interesting results as follows: penetrated surface oil (PSO) contributed mostly to the total oil content of all the fried products at 46% for WBD and 53% and 57% for WB and OB fried batter, respectively. Furthermore, oil distribution varied based on low-or high-moisture formulation. In fried batter (high moisture), the ranking is as follows: PSO > structural oil (STO) > surface oil (SO), while in the fried dough (low moisture) products, the ranking is PSO > SO > STO. Results confirm that oil uptake in the fried dough-based product (magwinya) is a surface-related phenomenon. However, the distribution of the oil fractions is moisture dependent.

Data Availability
e data used to support the findings of this study are included within the article.

Conflicts of Interest
e authors declare no conflicts of interest regarding the publication of this paper.