Physicochemical Evaluation of Diploknema butyracea Seed Extract and Formulation of Ketoconazole Ointment by Using the Fat as a Base

.e fat obtained from the ripened seeds of Diploknema butyracea is widely used as a vegetable oil in rural areas of Nepal. .is study was aimed for the physicochemical evaluation (acid value, iodine value, saponification value, peroxide value, ester value, pH, and liquefaction point) of the Diploknema butyracea seed extract (chyuri fat) and the formulation of 2%w/w ketoconazole ointment by using it as a base. All the physicochemical parameters were determined quantitatively by using the method of Indian Pharmacopoeia (IP), volume-I. By fusion method, 3 different formulations F-A, F-B, and F-C were prepared, in which different proportions of chyuri fat, polyethylene glycol 6000 (PEG 600), Tween 80, and propylene glycol were used as an ointment base. Various quality parameters such as spreadability, extrudability, viscosity, smoothness, pH, average fill weight, assay, content uniformity, accelerated stability, and drug release profiles were determined. HPLC was used for the determination of ketoconazole content in the ointment formulations. Physicochemical evaluation of the chyuri fat ensured its suitability for industrial purpose. .e active ingredient release profile of formulations F-A (87.71%), F-B (88.89%), and F-C (91.09%) after 5 hours were within acceptable range along with other parameters. Assay of the formulations F-A, F-B, and F-C were reported to be 103.01, 107.9, and 102.45%, respectively. Overall, evaluation of the formulation F-A, prepared by using chyrui fat only, gave satisfactory results and most of the parameters were statistically similar (p> 0.05) to the F-B and F-C formulated by incorporating a certain proportion of synthetic base. .us it can be concluded that chyuri fat can be the best alternative to replace the expensive synthetic base.


Introduction
Diploknema butyracea Roxb (family: Sapotaceae) is a medium-sized deciduous tree having a height of about 20 m [1,2]. e plant is native to the Himalayan region, widely distributed to Nepal, India, and Bhutan up to an altitude of 300-1500 m. It is popular with the name "Chyuri" in Nepal, "Indian-butter nut" in English, and "Chiura" or "Phulwara" in India [1,3]. Diverse parts of this plant have been used by ethnic groups of Nepal and India mainly for food and medicinal purpose [4]. Juice is prepared from the stem bark to treat digestive complications, helminthiasis, asthma, ulcers, leprosy, and rheumatoid arthritis [5]. Corolla is expressed to get the sweet juice that is eaten fresh or boiled to obtain syrup [6]. e most applicable part of the plant is the edible seed. Chyuri seed contains about 55% fat (chyuri butter), which is used as vegetable oil as well as massage oil for rheumatism, headache, acne, and boils [7]. e chyuri fat is a very useful natural emollient for cracked feet and hands in the winter season [5,7]. Similarly, chyuri fat has been utilized as butter for oil lamps, hair oil, and raw material for toilet soap. Moreover, many commercial organizations have done experiments to prepare many commercial products such as chyuri syrup, chyuri squash, chyuri jam, and chyuri cream [8].
Nowadays, interest in the study of plant-derived fat and oils is increasing tremendously to utilize them as a vehicle for diverse pharmaceutical topical preparations [5,9]. Delivery of the drug through the topical route has long been proved to be a promising approach due to the large surface area of the skin, ease of access, wide exposure to the circulatory and lymphatic system, and confined nature of the treatment [9]. Among different topical preparations, ointments are semisolid homogenous formulations prepared for external application to the skin or mucous membranes. Ointments are commonly used as emollients or to administer the active drugs on the skin for preventive, therapeutic, or protective purposes and where occlusion of the certain body part is required. Various antifungal drugs are available commercially in the form of different topical formulations such as ointment, cream, and powder [10].
Ketoconazole is one of the potent broad-spectrum antifungal drugs which belong to the synthetic imidazole group. It is lipophilic in nature and practically insoluble in water [11,12]. It is characterized as biopharmaceutical classification system (BCS) class IIb drug [13]. e drug has a significant therapeutic effect against systemic and superficial mycosis along with Candida, Malassezia, and dermatophyte [10,14]. It inhibits the synthesis of fungal ergosterol, a key constituent of the fungal cell membrane, resulting in cell death. Besides, it also interferes with the biosynthesis of fungal phospholipids, triglycerides, and oxidative enzymes, increasing the cellular concentration of the toxic hydrogen peroxide. For the treatment of Candida albicans infection, it prevents the formation of invasive mycelia from the blastopores [15].
Despite the increasing demand of topical drug delivery system (ointment, cream, gel, etc.), still many problems exist, such as chemical instability of the base, necessity of many excipients to ensure the stability, high cost for the production of synthetic base, and hypersensitivity reaction of the base to the skin. is dire condition has compelled us to explore the more successful natural source. Chyuri fat has a long history of economical and ethnomedicinal importance to different ethnic groups in Nepal and it has been used as one of the chief components in many commercial preparations by cosmetic, pharmaceutical, and soap industries [8]. Hence, this study aimed to evaluate the physicochemical parameters and formulate 2% w/w ketoconazole ointment by using chyuri fat as an ointment base along with analysis of the ointment quality and stability.

Plant Collection.
Healthy and mature fruits of the Diploknema butyracea were collected from Tansen, Palpa district, Nepal (1350 m above from the sea level), in July 2019. Identification and authentication of the plant were done with the help of an investigation officer (Ms. Pratikshya Chalise) of National Herbarium and Plant Laboratories, Godawari, Lalitpur, Nepal (ref. no. 075/076363). e voucher specimen was properly labeled and preserved in the Pharmacognosy Laboratory, Department of Pharmacy, Crimson College of Technology (specimen number: CCT/ HRB/2019-212).

Extraction of D. butyracea Seeds. Fruits of D. butyracea
were first separated into peel and pulp. Seeds were taken out from the pulp manually. All the seeds were peeled manually ( Figure 1) and were outspread over the clean filter paper, kept in the well-ventilated laboratory room 25°C for 2 weeks. Filter papers were changed every day to protect from the possible fungal contamination. After the complete drying, seeds were ground to powder of size as small as possible with the help of a grinding machine. e sticky coarse fine powder mass was then extracted by using a cold triple maceration method in which 200 g of dried powder was immersed with 1000 mL hexane in the conical flasks with frequent shaking for 1 day. e menstruum was strained; the marcs were pressed and then subjected to filtration. e process was continued for 3 times and all the filtrate was collected together. e filtrate was dried by using a rotary vacuum evaporator at 40°C to obtain thick, viscous fat ( Figure 2) which was again dried by using a vacuum desiccator. Finally, the dried extract was preserved at 4°C until use.

Physicochemical Evaluation of the Chyuri Fat.
Physicochemical analysis was carried out by adopting the methods described in the Indian Pharmacopoeia (IP) 2018 [16].
2.4.1. Acid Value. About 10 g of the sample was weighed in the round bottom flask and dissolved in 50 mL of the previously neutralized mixture of the 95% ethanol and ether (1 : 1 v/v). After that, a reflux condenser was connected and warmed slowly with frequent shaking to dissolve the sample. en, the sample solution was titrated with standardized 0.1 M potassium hydroxide and endpoint was detected. Phenolphthalein solution was used as an indicator. Finally, acid value was calculated from the following equation: where n is the volume of 0.1 M potassium hydroxide consumed (mL) and w is the weight of the sample (g).

Iodine
Value. Iodine value was determined by using the Hanus method. At first, 290.4 g of the sample was placed in a 300 mL of dry iodine flask and dissolved in 15 mL of chloroform. en, 25 mL of iodine monobromide solution was added slowly from the burette. e sample was kept in a dark place for 30 minutes with frequent shaking. After that, 10 mL of 30% w/v of potassium iodide solution and 100 mL of water was added followed by titration with standardized 0.1 M sodium thiosulphate using starch solution as an indicator during the end of the titration. e volume of 0.1 M thiosulphate (mL) consumed was noted as (a). e same operation was repeated without using sample, and volume consumed (mL) was noted as (b). e iodine value was calculated by using the following equation: 2.4.3. Saponification Value. About 2 g of the sample was kept into a 250 mL round bottom flask and fitted with a reflux condenser. After that, 25 mL of the ethanolic 0.5 M potassium hydroxide and pumice powder was poured and boiled over a water bath for 30 min. e sample was allowed to cool for a few minutes. Finally, the sample solution was titrated against 0.5 M hydrochloric acid (HCl) using phenolphthalein as an indicator. Blank titration was also performed without adding sample. e saponification value was calculated from the following equation: where w is the weight of the sample in g and b and a are the volume of the HCl in mL, consumed by blank solution and sample solution, respectively.

Peroxide
Value. About 5 g of the test sample was transferred into a 250 mL conical flask with a glass stopper, and the mixture of 3 volume of glacial acetic acid and 2 volume of chloroform was added gradually with continuous shaking and 0.5 mL of saturated potassium iodide solution was added. e homogenous sample solution was then allowed to stand for exactly 1 min with frequent shaking. After that, 20 mL of the distilled water was poured and subjected to titration against standardized 0.01 m sodium thiosulphate until the yellow color disappears. Furthermore, 0.5 mL of starch solution (5% w/v in water) was added drop-wise and titration was continued again with vigorous shaking until the blue color just disappears (a mL). Blank determination was also performed without a sample (b mL). Finally, the peroxide value was calculated by the following equation: where w is the weight of the sample in g.

Ester
Value. Ester value was calculated by using the following equation: 2.4.6. Liquefaction Point. e sample was loaded inside a fine capillary tube and the liquefaction point was determined by using digital melting point apparatus.

Formulation of the 2% w/w Ketoconazole Ointment.
ree different formulations (F-A, F-B, and F-C) of the ketoconazole ointment were prepared by the fusion method [17]. As shown in Table 1, the composition of the formulation was taken from the literature [17], with slight modification. In this technique, all the components were melted together in the decreasing order of their melting points. At first, seed butter was heated to 70°, and then PEG 600, propylene glycol, and Tween 80 were added gradually in it. After that it was cooled up to 40°C and ketoconazole was dissolved with continuous sonication assisted agitation for 30 min. Finally, the formulation was filled in aluminum tubes each containing 10 g of ointment, capped well. All the ointment tubes were stored properly in the air tight bag and bag was stored in a vacuum desiccator at dark and cool place until use.  2.6.2. Spreadability. Excess of sample in between two glass slides (10 * 10 cm) was placed and a certain load (2 kg weight) was placed at the center of the plate. e time required in second to separate the two slides was measured to determine the spreadability. If two slides separate in less time it indicates the good spreadability of the ointment. Spreadability was calculated by the following equation: where S is spreadability, M is the weight placed to the upper slide, L is the length of glass slide, and T is time taken to separate the slides [18,19].

Extrudability.
It gives the information about the force required to extrude the drug material from the tube. e higher the amount of drug extruded, the better the extrudability and quality of the formulation. In this study, the drug sample was filled in a clean collapsible aluminum tube having a nozzle tip with a 5 mm opening. e pressure was applied to the tube by using a finger. Finally, the percentage of the ointment extruded through the tip was determined by using the following equation [19]: % extrudability � amount of ointment extruded from the tube total amount of ointment filled in the tube × 100.
2.6.4. Average Filling Weight. e net weight of the formulation in the filled containers was determined to ensure that the product contains the uniform and proper amount of sample as labeled. For this analysis, a total of 10 filled tubes were taken and weighed. After that, all the contents of each tube were removed completely and the weight of the empty tube was taken. Finally, the net weight of the drug sample in each tube was calculated and the average fill weight was determined [18].
2.6.5. Washability. Drug sample was spread over the skin and then easiness of washing with water was observed. For this, 1 g of ointment was rubbed over the hand skin of three healthy humans. After 30 minutes, applied area was washed with normal tap water [17].
2.6.6. Nonirritancy Test. Ointment formulation was applied over the human skin to observe the undesired irritant effect [18].

Determination of pH.
e pH of the ointment solution was measured by using digital a pH meter. About 1 g of ointment was suspended in 100 mL of distilled water and kept for 2 h. e pH of the each formulation was measured three times.

Viscosity of the Ointment.
e viscosity of each formulation was measured by viscometer DV-III ULTRA Brookfield viscometer. About 20 g of the test sample was placed in a dry and clean 250 mL beaker. Measurement was done by using spindle no. 64. e data was expressed in centipoises unit (cps). e sample was rotated at 10 rpm for 1 minute. e temperature was maintained up to 25°C [20].

Determination of the Active Ingredient in the Formulation
(1) Optimization of the HPLC Condition. Quantitative determination of the active ingredient in each formulation was carried out by using the reverse phase HPLC system of prominence-i LC-2030 (Shimadzu, Japan). e chromatographic condition for the analysis was adopted from the method of analysis for ketoconazole raw material (USP 2018) [21].
(2) Method Validation. e validity of the method used for quantitative analysis of ketoconazole ointment formulation was confirmed by analyzing different parameters, namely linearity, limit of detection and quantification (LODs and LOQs), precision (intraday and interday variation and repeatability), and accuracy (recovery) according to ICH guideline [22] and previous study [23][24][25].
where sp is sample, std is standard, Wsp is the weight of the sample ointment taken (2.5 g), Wstd is the weight of standard drug taken (50 mg), and LOD is loss on drying for standard.
(4) Content Uniformity. Randomly 10 ointment tubes were taken from each formulation and assay of each tube was carried out by injecting the samples in HPLC to ensure uniform mixing of ketoconazole in each tube so that every ointment tube contains the labeled amount of active drug.

In Vitro Active Ingredient Release.
To investigate the in vitro active ingredient release pattern of the newly formulated ketoconazole ointments, a dialysis method was adopted [9,20]. In this method, 3 grams of each formulation was put down on cellophane (semipermeable membrane). e donor compartment which was loaded with an ointment sample was securely pulled up over the lower open end of a glass tube having an internal diameter of 2.5 cm. A rubber band was used to make watertight. e ointment-loaded tube was submerged in a beaker filled with 100 mL of acetic buffer of pH 5.5 (with 1% sodium dodecyl sulphate to promote the sink conditions), which acts as active ingredient release medium or receptor compartment.
e whole system was set with a magnetic stirrer, and the active ingredient release medium was regularly stirred by using magnetic beads at 50 rpm maintaining the temperature of 37 ± 0.5°C in a thermostatic water bath. After the different time intervals (0.5, 1, 2, 3, 4, and 5 hours), 1 mL of solution was withdrawn and immediately replaced with the same volume of fresh buffer by using a micropipette. e amount of the active constituent released from different formulations was determined by the HPLC method.
2.6.11. Accelerated Stability Studies. Stability of the newly formulated ointments was conducted under ICH guideline for accelerated conditions. For this, all the 3 formulations were stored in a stability chamber for 3 months. e condition of stability chamber was 40°C ± 2°C/75% RH ± 5% RH [26]. e samples were evaluated for ketoconazole assay, pH change, smoothness, and color change at the end of first and third month.

Statistical Analysis.
All the experiments were analyzed three times and the data were presented as mean ± SD. Statistical significance of differences was calculated by twoway analysis of variance (two-way ANOVA) and one-way analysis of variance (one-way ANOVA), with Tukey post hoc test using GraphPad Prism 6.0 software. A p value < 0.05 was considered to be significant.

Physicochemical Analysis of the D. butyracea Seeds Extracts (Chyuri Fat)
. Different physicochemical properties of the chyuri fat were determined to characterize its present condition and quality ( Table 2). Results from our data are compared with most physicochemical parameters of most commonly used vegetable oil (olive oil) [27]. e higher acid value indicates the greater amount of the free fatty acid present in the sample and the lower iodine value signifies that the proportion of saturated fatty acid is high. Similarly, higher saponification value, lower peroxide value, and higher ester value in this sample denote the presence of average molecular weight, less degradable, and higher numbers of saponifiable triglycerides.

Evaluation of the 2% w/w Ketoconazole Ointment
Formulations. After the preparation of 3 different formulations, their qualities were investigated in terms of different parameters (Table 3) and were found to be complied. All the formulations were white and viscous with smooth homogenous consistency. From the data of Table 3, it is obvious that all the formulations exhibited good spreadability and extrudability. Similarly, the pH of all the formulations is within the normal pH range of the human skin (5.5-7). Analysis of variance (ANOVA) indicated that the formulation C has significantly less spreadability and extrudability in comparison to formulation B (p < 0.05). Formulation C was found to be significantly most viscous among the formulations (p < 0.05).

Optimization of Chromatographic
Conditions. e chromatographic condition described by the USP 2018 for the analysis of active ingredient ketoconazole was tested for the newly formulated ketoconazole ointment. e method was verified for the analysis after ensuring the system suitability and its validity. All the peaks were detected at 225 nm. Figures 3 and 4 represent the chromatograms of blank and placebo, respectively. Similarly, the chromatogram of standard and test sample is depicted in Figure 5.

Method Validation.
e chromatographic system was examined for linearity, precision, specificity, and accuracy to confirm the selectivity, reproducibility, and preciseness of the finalized method. e regression coefficient (R 2 ) of the standard was 1; hence the linearity was confirmed. Complete      Journal of Food Quality information of the calibration curve, LODs, and LOQs is summarized in Table 4.

Journal of Food Quality
To confirm the precision of the finalized method, intraday and interday variations together with the repeatability of sextuplates were examined. e relative standard deviations (RSD) for the interday and intraday investigations were found to be in the range of 0.58-0.98% and 0.11-1.68%, respectively (Table 5).
For the repeatability test of ketoconazole, standard solution was injected six times in a same day. e RSD was reported to be 1.41% (Table 6). e recovery percentage was ranged from 95.33 to 104.73% with RSD value of 0.34-3.48% (Table 7).
In the system suitability examination, average NTP, average tailing actor, and RSD of the area were found to be 91656, 1.072, and 0.33, respectively, which are in compliance with given criteria of USP 2018.

Assay of the Ketoconazole Ointments.
In both initial and accelerated stability study, the active ingredient content of all three formulations was found to be within the limit (95-110%) specified by US Pharmacopoeia 2018. Slight variation in the active ingredient was found among three different formulations. As shown in Table 8, for all the batches decrease in active ingredient content after one month was statistically significant ((p < 0.05), whereas after 3 months, a significant decrease in active ingredient content was not observed (p > 0.05). Moreover, the content of the active ingredient after accelerated stability in all formulations was within the pharmacopoeial limit.

Content Uniformity of the Different Formulations.
To ensure the homogenous mixing of the active ingredient, ketoconazole content of the individual 10 tubes was calculated for each formulation. As shown in Table 9, ketoconazole content in each tube was reported to be within the pharmacopoeial limit.

In Vitro Active Ingredient Release.
Results of the in vitro active ingredient release evaluation by using the dialysis method are depicted in Table 10. For statistical analysis of active ingredient release percentage (%), we used two-way ANOVA to compare the active ingredient release pattern among the formulations in different time intervals, so two variable factors were 3 different formulations and different time intervals from 0.5 h to 5 h. e extent of the active ingredient released was proportional to the time increased. e release pattern of formulation A was almost similar to the F-B and F-C. is signifies that the formulation of the ointment by using chyuri fat only can ensure the desired active ingredient release of the formulation.

Discussion
e acid value of the fat gives the information about the amount of short-chain free fatty acid present in it. In most cases, it is the evaluation of the extent of triglycerides decomposition by lipase enzyme activity, light, and heat [28,29]. It gives an idea about the edibility of the fat. e fat or oil substance with an acid value higher than 4 mg/g is considered to be unsuitable for the edible process [30]. e higher acid value of chyuri fat (61.86 mg KOH/g) suggests that its consumption is not beneficial for human health. e iodine value gives information about the average degree of unsaturation. It is used to quantify the numbers of double bonds in the fat which are susceptible to oxidative degradation [31]. e lower iodine value of the chyuri fat (36.26 g I 2 /100 g) indicated that it contains a higher proportion of saturated fatty acids. Major fatty acids present in chyuri fat are methyl ester formed of saturated palmitic acid (66%), saturated stearic acid (2.4%), monounsaturated linoleic acid (26%), and polyunsaturated oleic acid (2.6%), indicating stability of the fat towards lipid peroxidation [8,32]. Fat or oil samples having the iodine value lower than 100 g I 2 /100 g are suitable for industrial uses [33] like bakery production,  x: concentration in μg/mL; y � peak area.    Note. Different superscript letters within the same row indicate significant differences (p < 0.05) among the formulation.  margarine production, cosmetic industry, and drug industry [34]. e low iodine value of any fat or oil ensures its stability against oxidation and rancidity of products prepared with it [33,34]. e stability of the fat against possible rancidity was also verified by the lower peroxide value (3.14 Meq KOH/g), obtained in this study. Peroxide value is an indication of possible autooxidation; hence low value ensures that it can resist peroxidation in the course of storage [31]. Similarly, the saponification value (225.05 mg KOH/g) and ester value (163.19 mg KOH/g) of the chyuri fat were reported to be high. e higher saponification and ester value indicates that fat contains a large proportion of short-chain lower molecular weight and saponifiable triglycerides, respectively.
is type of fat is suitable for cosmetic and industrial purposes [28,33]. Moreover, the liquefaction point (68°C) of this fat also ensures its suitability for the ointment base [35].
After the confirmation of its chemical stability to use as an ointment base, 2% w/w ketoconazole ointment was formulated by fusion method and quality was evaluated. Physically, all the ointment formulations were smooth viscous preparation without any grittiness and white. No skin irritations were observed after applications and all the formulations were easily washable. Also, the pHs of all the formulations were within the normal pH condition of the human skin (5.5-7) [17]. From the one-way analysis of variance (ANOVA), it was evident that viscosity of the formulation C was significantly high among all (p < 0.05). Also, the viscosity of formulation A was higher than B. Incorporation of the PEG 6000 and Tween 80 in the formulation C might be the contributing factor for its highest viscosity [17,20]. e higher viscosity of formulation A as compared to formulation B might be due to the higher proportion of chyuri fat in formulation A. Formulation B was found to be more extrudible and spreadable than other formulations and the difference in these parameters of formulations B and C were statistically significant (p < 0.05).
In vitro active ingredient release study is an important analytical step to evaluate the extent of active release from the medicinal product matrix in controlled laboratory condition during the process of drug development and quality control analysis [36]. e active ingredient release investigation of all the 3 formulations, conducted by using dialysis method (Table 10), revealed the satisfactory result. e two-way ANOVA test was applied to evaluate the statistical significance of release pattern. It was found that there was increase in the active ingredient release in every hour and was significant at p < 0.05. After 5 h, the active ingredient release profile of formulation C was more significant than A and B. is might be due to the addition of Tween 80 and PEG 6000. Formulation A was composed of ketoconazole (2%) and natural chyuri fat (98%). In formulation B, propylene glycol (16.66%) as a solubilizer [17] was added in addition to active drug (2%) and chyuri fat (81.33%). In the case of formulation C, Tween 80 (16.66%) as a surfactant [19] and PEG 6000 (16.66%) as a water solubilizing base [37] were also incorporated along with ketoconazole (2%), chyuri base (48%), and propylene glycol (16.66%). Ketoconazole release percentages of formulations A, B, and C were almost similar throughout the study. While comparing formulations A and B, the percentage of the active constituent was very slightly higher in formulation B, which may be due to the incorporation of propylene glycol. However, there was no statistically significant variation between these formulations (p > 0.05) except at 1 and 2 hours (p < 0.05). Despite the higher viscosity of formulation C, active ingredient release profile was slightly better than A and B. is might be due to the addition of Tween 80 and PEG 6000. is is because Tween 80 improves the uniform solubilization of drug all over the vehicle [19] and PEG 6000 enhances penetration of water to promote rapid release of active principle [37]. Due to its high polarity, PEG play significant role in increasing the hydrophilicity and thus enhances water solubility. erefore, it is widely used in ointment for solubilization and permeation [37]. e active ingredient release pattern of F-A which was almost similar to the F-B and F-C, which signifies that the formulation of the ointment by using chyuri fat only can ensure the desired release of active constituent.
To confirm the homogenous mixing of the active ingredient in all formulations, 10 randomly selected tubes from each formulation were taken and ketoconazole content was determined. Content of ketoconazole in formulations A, B, and C ranged from 101.72-103.89%, 102.55-104.80%, and 102.77-104.92%, respectively. All the formulations were within the pharmacopoeial range. e formulated ointments were investigated for accelerated stability study according to ICH guidelines for up to three months. e results of accelerated stability studies revealed that no formulation suffered from evident physical and chemical change (color, phase separation, smoothness, and pH). Content of the active constituent that decreased for formulation B over the 3-month stability showed significant change (107.9-97.05%) in comparison to formulation A (103.01-98.55%) and formulation C (102.45-97.7%). However, assay of all the formulation was within the pharmacopoeial limit (90-110%) [21] after 3 months of accelerated study. Further advance stability investigations, which were beyond the range of this study, are required to determine the shelf-life of these formulations.

Conclusions
In summary, the present research work evaluated the physicochemical parameters (acid value, iodine value, saponification value, peroxide value, ester value, pH, and liquefaction point) of chyuri fat and it concluded that the fat is highly recommended for the industrial, cosmetic, and pharmaceutical purpose. e high acid value suggested its unsuitability for the edible use. Furthermore, the ointment formulation F-A which was prepared by using chyrui fat only, for the first time, gave satisfactory results related to its physical and chemical stability. Various evaluation parameters such as spreadability, extrudability, viscosity, average fill weight, active principle content, content uniformity, accelerated stability, and active ingredient release profile were within the acceptable range and almost similar to the F-B and F-C in which synthetic base was incorporated. us, further extensive study is recommended so that chyuri fat can be established as a better alternative for other synthetic base and its commercialization may improve the socioeconomic status of the local people.

Data Availability
All the data used to support the result of this research are available from R. Bhandari and J. Pandey upon request.

Disclosure
is research project was performed as part of the partial fulfillment of academic degree at Crimson College of Technology, Nepal.