Fabrication and Characterization of ZnO Nanowire Arrays with an Investigation into Electrochemical Sensing Capabilities

ZnO nanowire arrays were grown on a Si (100) substrate using the vapor-liquid-solid (VLS) method. ZnO nanowires were characterized by XRD, SEM, bright field TEM, and EDS. They were found to have a preferential orientation along the c-axis. The asprepared sample was functionalized with glucose oxidase by physical adsorption. FTIR was taken before and after functionalization to verify the presence of the attached enzyme. Electrochemical measurements were performed on the nanowire array by differential pulse voltammetry in the range of−0.6 to 0.4 V. The nanoarray sensor displayed high sensitivity to glucose in the range of 1.0×10−4 to 1.0 ×10−2 mol L−1.

Miniaturization is one ongoing important development in biosensor technology.Miniaturization, however, may result in low current because of the decreased amount of immobilized enzyme onto the available active area.It has already been reported that nanostructures can enhance the sensitivity of a biosensor by one to two orders of magnitude, due to the large surface area per unit volume ratio, which allows the immobilization of a larger amount of the enzyme.Since the development of the first glucose sensor enzyme electrode performance, stability and selectivity have been a main thrust for further research [15].The incorporation of biomolecules into carbon nanotubes (CNTs) and metal oxide nanowires is achieved through various methods of immobilization such as covalent linkage [16], entrapment [17], cross-linking with glutaraldehyde [18], microencapsulation [19], and adsorption [20][21][22].Adsorption is one of the more common schemes of immobilization because it is a method that requires minimal preparation.In this work, prolonged exposure of glucose oxidase to ZnO nanowires has resulted in enzyme immobilization through nonspecific adsorption of the enzyme on the sidewalls of the nanowires.This letter reports on the synthesis and characterization of ZnO nanowires by vapor-liquid-solid (VLS) mechanism and its application as an electrode for glucose measurement without any additional protective coating.
A high temperature furnace (Lindberg/Blue) was used for the growth of ZnO nanowires.As synthesized products were characterized by X-ray diffraction with Cu-Kα radiation (Philips X'pert Pro diffractometer), field emission scanning electron microscopy (FE-SEM, Hitachi S-800), and highresolution transmission electron microscopy (FEI Tecnai F30, HR-TEM).TEM specimens were prepared by ultrasonicating the ZnO nanowires in methanol and dispersing a drop of solution on a carbon-coated copper grid.Chemical compositional analysis was carried out by EDX coupled with the HR-TEM system.
Electrochemical experiments were performed using a Princeton Applied Research PARSTAT 2263 advanced electrochemical analyzer.All electrochemical measurements were executed in a standard three-electrode system at room temperature.The modified zinc oxide sample acted as the working electrode, with an Ag/AgCl (3 M KCl) reference electrode, and a platinum wire (CH Instruments, Tex, USA) counter electrode.All potentials given in this paper are relative to the Ag/AgCl electrode.The pH of the glucose solutions was measured with a Fisher Scientific AB15 pH meter.FTIR studies were performed on a Perkin-Elmer Spectrum One FT-IR Spectrometer.
For the fabrication of a glucose sensor, we have initially grown an array of ZnO nanowires on Si (100) via thermal evaporation, with the use of a gold catalyst.Freshly prepared ZnO source powder and substrates were loaded in two different alumina boats in the high-temperature and lowtemperature zones of the vacuum furnace, respectively.The furnace was initially evacuated to a pressure of 10 −3 Torr and argon was then passed at a constant flow rate of 500 sccm.The temperature of the furnace was approximately raised to 900 • C-950 • C. The substrates were unloaded after the furnace was cooled to room temperature.The zinc oxide nanowire array was then functionalized with the enzyme glucose oxidase.Approximately 15 IU of GOX was applied onto the nanowire surface via physical adsorption.The newly constructed electrode was allowed to dry over 24 hours at room temperature prior to use.

RESULTS AND DISCUSSION
The X-ray diffraction pattern of the as-grown ZnO products is shown in Figure 1.All the visible peaks are indexed to a wurtzite (hexagonal) structure of ZnO with lattice constants of a = 0.3250 nm and c = 0.5205 nm, respectively [23].A small shift was observed in the peaks of ZnO nanowires when compared to ZnO bulk.This might be due to the thermal stresses developed at the time of growth.In addition, Au (111) and Au (200) peaks were also detected from the XRD pattern.The high intensity of (002) peak of ZnO nanowires shows that the preferential growth direction is along the caxis.The surface morphology of the patterned sample can be observed in the SEM images (see Figure 2).The ZnO nanowires have a typical length of 0.5-2 μm and a diameter of 40-120 nm. Figure 3 shows the TEM image of a pair of nanowires and inset shows the electron diffraction pattern of the wires.It is clearly shown from the electron diffraction pattern that the one-dimensional nanowires were single crystal and grown along [0001].A representative energy dispersive X-ray (EDX) spectrum was performed near the tip of the ZnO nanowire as indicated by the arrow shown in Figure 3(c).The peaks associated with Zn, O, Au, Cu, and C are seen in the EDX spectrum, where the peak corresponding to Au confirms that the tips of the nanowires were encapsulated with a gold particle of diameter ∼52 nm (see Figure 3(c)) and the copper and carbon signatures are from the carbon-coated copper TEM grid.
The as-grown ZnO nanowires on silicon substrate were analyzed by Fourier transform infrared (FTIR) spectroscopy before and after functionalization with GOX (see Figure 4).The absorption peak at about 1000 cm −1 can be interpreted as the Si-O-Zn vibrational mode [24].GOX is seen through the presence of the primary amine group.The N-H bending is observed at 1600 cm −1 while the N-H stretch due to asymmetric and symmetric vibrations occurs at 3400 cm −1 and 3300 cm −1 , respectively.The activity of the enzyme glucose oxidase is affected by the pH of the glucose solution.The pH dependence of the sensor was evaluated at 5 mM glucose solutions in the range of pH 6 to 9 (see Figure 5).An optimal peak current of the sensor was displayed at pH 6.5.Considering that the pH of human blood is about 7.4, the amperometric experiments were performed at pH 7.0.Figure 6 shows the cyclic voltammograms of the ZnO-GOX electrode in PBS at a pH of 7.0 and at room temperature.The inset shows the plot of peak current versus the square root of the scan rate.The plot is nearly linear with less than 3% error from 50 to 400 mV s −1 .The decrease in current response    with successive increase in scan rate indicates that the electrode reaction is diffusion controlled.The direct pulse voltammetry (DPV) response of the sensor to successive increments of glucose is shown in Figure 7(a).These results were obtained with a scan rate of 0.020 mV/s, step height

CONCLUSIONS
The successful fabrication of a highly selective ZnO nanowire-based amperometric glucose biosensor has been achieved.The ZnO electrodes were synthesized on Si (100) substrates by VLS mechanism.High-density ZnO nanowires with a large surface area are found to have a preferential growth direction along [0001] axis.No additional protective coating has been utilized during the electrode preparation.The sensor functioned in the range of 1.0 × 10 −4 to 1.0 × 10 −2 mol L −1 glucose.The biosafe nature of ZnO and successful immobilization of glucose oxidase onto the electrode surface leads to a new novel approach to biosensor construction and applications.

Figure 1 :
Figure 1: (upper curve) X-ray diffraction spectrum of an array of ZnO nanotubular structures and (lower curve) bulk ZnO, respectively.

Figure 2 :
Figure 2: (a) Low magnification top-view SEM image of patterned ZnO nanotubes.(b-d) Side view of patterned-and aligned-ZnO nanotubes from lower to higher magnification (clockwise direction).

Figure 3 : 2 − 1 − 1 0
Figure 3: (a) Bright field TEM image of a pair of ZnO nanotubes, inset shows electron diffraction pattern on a pair of ZnO nanotubes along the zone axis [2 − 1