One-Step Method for Preparation of Magnetic Nanoparticles Coated with Chitosan

Preparation of magnetic nanoparticles coated with chitosan in one step by the coprecipitation method in the presence of different chitosan concentrations is reported here. Obtaining of magnetic superparamagnetic nanoparticles was confirmed by X-ray diffraction and magnetic measurements. Scanning transmission electron microscopy allowed to identify spheroidal nanoparticles with around 10-11 nm in average diameter. Characterization of the products by Fourier transform infrared spectroscopy demonstrated that composite chitosan-magnetic nanoparticles were obtained. Chitosan content in obtained nanocomposites was estimated by thermogravimetric analysis. The nanocomposites were tested in Pb2+ removal from a PbCl2 aqueous solution, showing a removal efficacy up to 53.6%. This work provides a simple method for chitosan-coated nanoparticles obtaining, which could be useful for heavy metal ions removal from water.


Introduction
Magnetic iron oxides, like magnetite (Fe 3 O 4 ) and maghemite (γ-Fe 2 O 3 ) are a widely known materials because of their superparamagnetism when the particles are smaller enough [1].This property, along their low toxicity and a great surface/area ratio, makes magnetic nanoparticles attractive for a number of actual and potential applications [2][3][4][5][6][7][8].For applications in biomedical and environmental fields, magnetic nanoparticles are usually coated with polymers, bounded to the particle through organic linkers [9].This type of coating is able to recognize specific molecules and ions for their binding and removal.Furthermore, in some cases it offers enhanced stability [10].
Chitosan is a partially deacetylated form of chitin obtained by thermochemical treatment.It has been identified as a versatile biopolymer, nontoxic, and biodegradable, which is used for a number of agriculture, food, and biopharmaceutical applications [11,12].Free amino and hydroxyl groups are responsible for the reactivity of this polymer.Magnetic nanoparticles coated with chitosan can provide a convenient tool for exploring separation techniques as well as novel applications in fields such as protein and metal adsorption [2,13,14].
An attractive option to obtain this type of particles is carrying out precipitation and coating in one step.To our best knowledge, there are very few reports on this subject [19,20].Hong and Rhee [19] prepared chitosan-coated magnetic nanoparticles with 28 wt.% in theoretical chitosan content by coprecipitation in a solution containing Fe +2 , Fe +3 , and chitosan.However, particle size as determined by quasielastic light dispersion was relatively large, 67 nm in average diameter, despite that uncoated magnetic nanoparticles were very small, with average diameter around 7.5 nm.The cause of this increase in size was not discussed in the paper.Furthermore, additional characterization of coated nanoparticles was not included.Rather, the attention was focused on establishing the effect of chitosan-coated magnetic nanoparticles on the relaxation times of hydrogen protons of water molecules in a dispersion of this type of particles in water.On the other hand, Wu et al. [20] reported the in situ preparation of chitosan-coated magnetic nanoparticles for lipase immobilization.The nanoparticles were obtained by precipitation of Fe(OH) 2 and further controlled oxidation to Fe 3 O 4 in an aqueous solution containing crosslinked chitosan.It is noteworthy that the weight ratio chitosan in the formulation to the theoretical Fe 3 O 4 was very high (≈8.7).In contrast with the report of Hong and Rhee [19], Wu et al. [20], based on the characterization by vibrating sample magnetization and the thermogravimetric analysis of the nanoparticles, concluded that chitosan-coated magnetic nanoparticles were prepared.According to the measurements by transmission electron microscopy, particle diameters of Fe 3 O 4 ranged between 10 and 20 nm and those of chitosancoated magnetic nanoparticles, between 50 and 100 nm.
We report in this paper the coprecipitation of magnetic nanoparticles in the presence of different chitosan concentrations.A detailed characterization of the products was carried out to demonstrate the feasibility of this method for obtaining chitosan-coated magnetic nanoparticles in one step.Moreover, the kinetics of interaction between selected nanocomposites and Pb +2 ions was determined to evaluate their ability to ions removal.

Preparation of Coated Magnetic
Nanoparticles.The process was carried out in a 150 mL jacketed glass reactor equipped with a reflux condenser, mechanical agitation (400 rpm), and an inlet for the feed of aqueous ammonia.All the reactions were performed in duplicate (set 1 and set 2).The procedure started with mixing in the reactor 50 mL of FeCl 3 •6H 2 O (0.32 M) and 50 mL of FeCl 2 •4H 2 O (0.2 M).Then, the required amount of chitosan was added to the reaction mixture, raising the mixture temperature to 50 • C. Three concentrations of chitosan were used: 0.125, 0.25, and 0.5% (w/v).Coprecipitation reaction without chitosan was also carried out as a control.Once Fe +2 , Fe +3 , and chitosan solution reached the desired temperature (50 • C), the dosing of 20 mL of the aqueous ammonia at 0.67 mL/min was started.After dosing was over, the completion reaction was allowed to proceed for 20 min.At the end of the reaction, the particles were recovered by using a permanent magnet, washed 25 times with de-ionized water, and lyophilized to obtain the final product.

Nanocomposites Characterization.
X-ray analysis of the products were carried out with a Siemens D-5000 diffractometer using Cu-K α (λ = 1.5418Å) as incident radiation.The size and morphology of the particles were determined in a JEOL JSM-7401F scanning-transmission electron microscope (STEM), for which samples were prepared by dispersing the resulting powders in water with ultrasonication and then depositing the dispersion on a copper grid.The magnetic properties of the nanoparticles were determined using a Physical Properties Measurement System from Quantum Design, model 6000 in mode-vibrating sample magnetometer (VSM), with an applied field between −20.0 to 20.0 kOe at room temperature.Attenuated total reflectance (ATR) Fourier transform infrared spectrometry (FTIR) was carried out in a Magna IR 550 from Nicolet with germanium crystal.Thermogravimetric analysis (TGA) of nanocomposite and chitosan was performed in a TGA Q500 from TA Instruments.Analyzed samples were heated from 30 to 800 • C at a heating rate of 10 • C/min under a nitrogen flow of 50 mL/min.

Pb +2
Removal by Using Chitosan-Coated Magnetic Nanoparticles.Typically, 60 mg of dried nanoparticles were added to 50 mL of 10 ppm Pb +2 aqueous solution.Then, this mixture was ultrasonicated for 50 min at room temperature taking samples during the process each 10 min.After the magnetic chitosan nanocomposites were removed, the concentration of Pb +2 in the samples was measured by atomic absorption spectroscopy in a Varian Spectra 250 AA equipment.

Results and Discussion
All reactions rendered a black powder at the end of the process.There was no visually detected difference between the nanoparticles prepared with and without chitosan.Figure 1 shows the X-ray diffraction pattern (XRDP) of the final products obtained in set 1 of precipitation reactions.XRDP of the products from set 2 (not included) shows the same signals pattern.As is well-known, precipitation reactions from Fe +2 + Fe +3 aqueous solution using aqueous ammonia usually produce a mixture of magnetite and maghemite.To compare with the obtained results, the standard patterns of these species, taken from the library of our X-ray equipment, were included in Figure 1.From this figure, it is evident that product patterns match pretty well those of maghemite and magnetite.Although differentiation between both species is not possible from the X-ray data in Figure 1, magnetite presence is evident due to the black color of the final products.It is noteworthy that there is no difference in the patterns of the products obtained with and without chitosan, which leads to conclude that the presence of this polymer does not affect the crystallinity of the precipitation reaction products.
The average grain sizes of the magnetic nanoparticles obtained in set 1 and 2 of precipitation reactions were calculated by using data from their XRDPs and the wellknown Scherrer equation, which is represented as where d is the average diameter of grain in nm; K is the dimensional factor (0.9); λ is the X-ray wavelength (0.154 nm); β is the line broadening at half the maximum intensity in radians and θ is the Bragg's angle.Calculated values of average diameter of grain were 11.1 ± 0.6, 11.6 ± 1.3, 12.6 ± 0.5, and 10.6 ± 0.3 for products prepared with 0, 0.125, 0.25, and 0.5% (w/v) chitosan, respectively.These results indicate an absence of effect of chitosan on the grain size of magnetic nanoparticles.Micrographs of the final products prepared with and without chitosan (set 1) are shown in Figure 2. Particles in this figure show a spheroidal morphology, but a difference between the particles obtained with and without chitosan is not evident.To characterize these particles, the sizes of more than 1000 of them were measured from different micrographs of each of the samples using an image analysis program (ImageJ 1.37 c).The results of this analysis were presented as the corresponding histograms of particle diameters and included in Figure 2. Furthermore, the numberaverage diameter (D n ), the weight-average diameter (D w ), and the polydispersity index (D w /D n ) were calculated by using the following equations [21]: where n i is the number of particles of diameter d i .
D n and polydispersity values from products (set 1) are shown in Table 1.It should be clarified that the data in this table correspond to the sizes of magnetic nanoparticles, that is, the nanoparticles without chitosan coating.As it can be seen, D n of magnetic nanoparticles obtained in this study when chitosan was used in their preparation ranges from 9.9 to 11 nm, a very small range to consider that a direct relation between the particle size and the chitosan used in the formulation exists.It is noteworthy that these particle diameters are smaller than those reported in the preparation of magnetic nanoparticles by coprecipitation method, which ranges from 14 [17] to 25-30 nm [13,15,16].Although, it cannot be excluded that other authors using this method have obtained magnetic nanoparticles with similar sizes to those obtained in this work, they were not identified in the search in the specialized literature.For comparison, the values of grain average diameters calculated with Scherrer equation are also included in Table 1.Data in this table indicate that sizes from X-ray information and those measured by STEM are very similar.This suggests that no aggregation of the grains during magnetic nanoparticles forming was occurred, even in the presence of chitosan.
To this point, it is clear that a mixture of magnetitemaghemite nanoparticles was obtained in presence of chitosan.It is assumed that at least part of chitosan used in the process is coating these nanoparticles.This assumption comes from the expected bond between the hydroxyl groups in the chitosan and the oxygen atoms of Fe 3 O 4 (magnetite) and Fe 2 O 3 (maghemite).Unfortunately, it was not possible to demonstrate this assumption by STEM.Nevertheless, in the following, the obtaining of a composite chitosan-magnetic nanoparticles will be demonstrated.The results of magnetic measurements of the nanoparticles prepared in set 1 are depicted in Figure 3. None of the curves in this figure shows magnetic saturation up to 20 kOe, which is understandable taken into account that the surface atoms in very small particles, which constitute a great fraction of the total atoms, have difficulties for aligning the magnetic moments in the direction of applied magnetic field [22].The results of magnetic characterization of nanoparticles from set 2 (not shown) are very similar to those described for nanoparticles from set 1. Table 2 shows the mean values of the magnetic properties for the products obtained in precipitation reactions (set 1 and set 2).
Obtained results (Figure 3 and Table 2) demonstrate that nanoparticles prepared without chitosan are characterized by the highest final magnetization value (70.1 emu/g) and that this value decrease between 66.4 and 45.1 emu/g, as chitosan content in the precipitation reactions increases.Furthermore, the very small values for remnant magnetization (2.3-4.6 emu/g) and coercivity field (26.9-64.4Oe) suggest a superparamagnetic behavior of all the nanoparticles prepared in this study [23].In fact, this behavior is typical of magnetite and maghemite nanoparticles smaller than 10-15 nm in diameter [23].The decrease of the nanoparticles final magnetization as the chitosan content used in the precipitation reactions increased indicates that the proportion of the nonmagnetic material (in this case, chitosan) in nanoparticles increased.A comparison of the results of magnetic characterization obtained in this study with those reported in the specialized literature indicates that the final magnetization value of the naked particles prepared in this study is higher than those reported by Ge et al. [17] and Wu et al. [20].Furthermore, they are similar to that obtained by Pan et al. [16] but lower than those reported by Liu et al. [13] and Kuo et al. [18], who obtained values of magnetic saturation around 80 emu/g.Nevertheless, due to the relatively large size of the nanoparticles (≈25 nm in average diameter) obtained by Liu et al., they presented a ferromagnetic behavior, which is not attractive for some applications where reuse of nanoparticles is required, because their high values of remnant magnetization.Regarding to chitosan-coated nanoparticles, the final magnetization values obtained in this study are similar to those reported by other authors [16][17][18] and higher than that reported by Wu et al. [20], who obtained a value of 35.5 emu/g.It is noteworthy the relatively high values of final magnetization were obtained in this study, despite that the sizes of magnetic nanoparticles are in the bottom of the sizes range reported in the literature on preparation of magnetic nanoparticles by coprecipitation method [13,[15][16][17][18][19][20].In accordance with the known fact that the magnetization of small particles (diameters smaller than ca.15 nm) decreases as particle size decreases [24,25], smaller values of magnetization should be expected.Based on the reported direct relation between crystallinity and magnetization in magnetic particles [26], the higher magnetization values obtained in this study could be tentatively ascribed to an improved crystallinity of the magnetic nanoparticles.
Conclusive evidence of the obtaining of chitosan-coated magnetic nanoparticles in this study arose from FTIR results (Figure 4).This figure includes the IR spectra for all the prepared nanoparticles (set 1).In accordance with Figure 4, the characteristic absorption bands for chitosan appeared at 3363 (O-H and N-H stretching vibrations), 2874 (C-H stretching vibrations), 1653 (N-H bending vibrations), and 1070 cm −1 (C-O-C stretching vibrations) [27,28].The spectra of the products obtained using chitosan show the four characteristic absorption bands for this polymer.Taken into account that the products of the reactions were exhaustively washed and magnetically recovered, it was concluded that all the chitosan in the final products are chemically bound to the magnetic nanoparticles.Results of FTIR demonstrated that the precipitation reactions carried out with chitosan led to obtain a composite chitosan-magnetic nanoparticles.The results of characterization by TGA of the products obtained in set 1 (Figure 5) were used for the estimation of the amount of chitosan immobilized on the nanoparticles.As it can be seen in this figure, the product prepared without chitosan, that is, the mixture magnetite-maghemite, showed 2.16% in weight loss when it was heated from 30 to 800 • C. Pan et al. [16] reported that a sample of Fe 3 O 4 lost 1.3 wt.% when it was heated from 30 to 300 • C in a TGA equipment under similar conditions to those used in this study.As indicated by Pan et al. [16], this weight loss can be ascribed to the removal of free and chemically adsorbed water.On the other hand, a sample of pure chitosan, whose thermogram was also included in Figure 5, showed a weight loss of 69.4%.As it was expected, the composites showed weight losses intermediate between those of the mixture magnetite-maghemite and pure chitosan: 10, 11, and 27% for the products prepared with 0.125, 0.25, and 0.5% (w/v), respectively.Using these results and the weight of the samples before and after TGA characterization, the quantities of chitosan immobilized on the nanoparticles were estimated (Table 3).For comparison, the theoretical values of immobilized chitosan calculated from the recipe of precipitation reactions, assuming that the reactions proceeded to 100% and only magnetite was obtained, were also included in Table 3.In accordance with the data in this table, the amounts of chitosan immobilized on the nanoparticles estimated from TGA measurements were consistently higher than the theoretical values.This difference can be explained if the precipitation reactions do not proceed to 100%, which would lead to increase the chitosan/nanoparticles ratio.
Having demonstrated the preparation of magnetic nanoparticles with average diameters around 10-11 nm covered with chitosan in one step, the final test in this study was to evaluate the efficacy of the obtained composites in Pb +2 removal from a Pb(NO 3 ) 2 aqueous solution.This test is based on the ability of chitosan to chelate heavy metal ions through its amino groups.For this, the composites prepared with 0.125 and 0.5% (w/v) chitosan in set 1 were evaluated.Figure 6 shows that the Pb +2 concentration drops from its initial value (10 ppm) to around 6.2 in the first 10 minutes in the assay when the composite was prepared with 0.125% (w/v) chitosan.Then, it slowly decreases to attain a final value of 4.6 ppm at the end of the test.In the assay carried out with the composite prepared with 0.5% (w/v) chitosan, values of 8.3 and 6.0 ppm were attained in the first 10 minutes and at the end of the assay, respectively.From here, a removal efficacy of 53.6% for the chitosancoated magnetic nanoparticles prepared with 0.125% (w/v) chitosan and of 39.8% for those prepared with 0.5% (w/v) were calculated, which is surprising, because of a direct relation between removal efficacy and chitosan content in the evaluated composites was expected.This indicates that the nanoparticles prepared with the higher chitosan content have a smaller total amount of amino groups on their surface, which really means that they have less total surface area than the nanoparticles prepared with 0.125% (w/v) chitosan.A possible explanation for this behavior is that contents of chitosan as higher as 0.5% (w/v), under the conditions used in this study, favor the aggregation of chitosan-coated nanoparticles.In turn, this would lead to an increase in the final nanocomposite size and, as a consequence, to a decrease in the total surface area.Finally it should highlight the difference between the productivity in this study and those in the other reports on preparation of chitosan-coated magnetic nanoparticles in one step [19,20].Calculations based on the formulations in the reports of Hong and Rhee [19] and Wu et al. [20] indicate theoretical productivities of around 0.1 g of magnetic nanoparticles per 100 g of reaction mixture.In contrast, theoretical productivity in this study is around 1.7 g per 100 g of reaction mixture.Furthermore, the weight ratio chitosan in the formulation/theoretical magnetic nanoparticles is close to 0.4 and 8.7 in the reports of Hong and Rhee and Wu et al., respectively.These values are higher than those calculated in this study, which range from 0.06 to 0.24, depending on the used formulation.The highlighted differences indicate that the preparation of chitosan-coated magnetic nanoparticles in one step is a method able to attain relatively high productivities.Also, they suggest that an optimal ratio chitosan/magnetic nanoparticles should exist, which would lead to a minimization of particle aggregation.

Conclusions
A one-step method comprising the coprecipitation of magnetic nanoparticles in the presence of different chitosan concentrations allowed to obtain chitosan-coated magnetic nanoparticles with average diameter around 10-11 nm, as demonstrated by X-ray diffraction, magnetic measurements, STEM, and FTIR.The ratios chitosan/magnetic nanoparticles were estimated, from the results of TGA, between 135.9 and 584.8 mg/g.When evaluated for Pb +2 ions removal from a Pb(NO 3 ) 2 aqueous solution, chitosan-coated magnetic nanoparticles showed up to 53.6% in removal efficacy.This value corresponds to the composites with the lower chitosan content.It is believed that particle aggregation and, as a consequence, a decrease in the total surface area was favored when a higher chitosan content was used during the magnetic nanoparticles precipitation.

Table 1 :
Average diameters and polydispersity index of particles prepared by coprecipitation method determined: (a) from XRD data; (b) and (c) from STEM micrographs.

Table 2 :
Results of magnetic characterization of nanoparticles obtained at 50 • C without chitosan and with different chitosan concentrations.

Table 3 :
Immobilization of chitosan on the nanoparticles obtained by coprecipitation method at 50 • C.
a Calculated from the recipe of precipitation reactions.b Estimated from TGA results.