Journal of Nanomaterials
Volume 2015 (2015), Article ID 586904, 9 pages
http://dx.doi.org/10.1155/2015/586904

Research Article

Preparation and Characterization of Some Nanometal Oxides Using Microwave Technique and Their Application to Cotton Fabrics

M. Gouda,1,2 A. Aljaafari,¹ Y. Al-Fayz,¹ and W. E. Boraie1,3

¹College of Science, King Faisal University, Al-Hassa, Saudi Arabia
²Textile Research Division, National Research Center, Dokki, Cairo, Egypt
³Chemistry Department, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt

Received 20 September 2014; Accepted 2 February 2015

Academic Editor: Nurit Beyth

Copyright © 2015 M. Gouda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

The objective of this paper is the synthesis of some nanometal oxides via microwave irradiation technique and their application to augment multifunctional properties of cotton fabric. Cotton fabrics containing nanometal oxides were prepared via a thiol-modification of cotton fabric samples and then dipped into the metal salt solutions precursors and transferred to the microwave oven. The surface morphology and quantitative analysis of the obtained modified cotton fabrics containing nanometal oxides were studied by scanning electron microscopy coupled with high energy dispersive X-ray (SEM-EDX). The shape and distribution of nanometal oxide inside the fabric samples were analyzed by transmission electron microscopy of cross-section fabric samples. The iron oxide nanoparticles had a nanosphere with particle size diameter 15–20 nm, copper oxide nanoparticles had a nanosphere with particle size diameter 25–30 nm, and cobalt oxide nanoparticles had a nanotube-like shape with a length of 100–150 nanometer and a diameter of ~58 nanometer, whereas the manganese oxide nanoparticles had a linear structure forming nanorods with a diameter of 50–55 nanometer and a length of 70–80 nanometers. Antibacterial activity was evaluated quantitatively against gram-positive bacteria such as Staphylococcus aureus and gram-negative bacteria such as Escherichia coli, UV-protection activity was analyzed using UV-DRS spectroscopy, and flame retardation of prepared fabric samples was evaluated according to the limiting oxygen index (LOI). Results revealed that the prepared fabric sample containing nanometal oxide possesses improved antibacterial, LOI, and UV-absorbing efficiency. Moreover, the metal oxide nanoparticles did not leach out the fabrics by washing even after 30 laundering washing cycles.

1. Introduction

Recently, much attention has been paid to the preparation and applications of nanometal oxides onto cotton substrate due to their promising applications [1–4]. The nanometal oxides deposited on cotton fabrics have shown splendid antimicrobial behavior towards gram-positive and gram-negative bacteria [4–7]. The researchers also indicated that the coated fabrics show enhanced protection against UV-radiation [6]. There is an increase in the cognizance around the usage of antiseptic textiles in the practice of therapeutic garments, protecting clothes, and bedspreads to reduce the chance of disease originating in a hospital infections [3, 8]. Metal nanoparticles are greatly more effective than bigger particles for the reason of their greater surface area. They moreover show distinctive physical and chemical properties [9]. Textile fabrics treated with silver nanoparticles to enhance antimicrobial activity have been developed [10]. Nanocopper oxides are very effective in adding antibacterial property to fabrics [4, 5]. Furthermore, applications of these antibacterial fabrics on to the skin of animal confirmed that the fabrics do not have skin irritation properties [11]. Three common methods existed to precipitate the nanometal oxides into the cotton fabrics. First method was that nanometal oxides were prepared and then coated onto cotton fabrics just by pad-dry-cure technique [12, 13]. The second method was to apply the ultrasonic radiation as an active system for the precipitation of nanometal oxides onto the surface of cotton fabrics and further substrates [4, 5, 14, 15]. The third method was via thermal chemical treatment [3].

Moreover, the utilization of nanomaterials in textile materials has involved significant attention owing to their unique physicochemical goods and their prospective uses. For instance, nano-TiO₂ was used to augment of multifunctional properties of cotton fabrics, for example, ultraviolet-blocking, self-cleaning, and antiseptic properties [16–21]; ZnO nanoparticles have been applied in the textile industry for UV-protection and antibacterial assets and utilized in medical garments and shielding textiles [20, 21]. For an extended period, silver nanoparticles are nonhazardous, strong antiseptic which can eliminate more than 650 bacteria, virus, and fungi types. Consequently, silver nanoparticles interest a growing extent of scientific and manufacturing attention from fields such as textile science [21, 22].

In recent years, noble metal nanoparticles have been used in antibacterial textile finishing. The ions which are produced from metal nanoparticles interact with sulfur-containing proteins in bacterial cell, which influence bacterial cell viability [23]. The noble metal nanoparticles slowly oxidize to release ions that react with the bacterial cells. This process conveys strong antibacterial effects toward pathogenic microorganisms for a long period.

Furthermore, direct synthesis/growth of metal nanoparticles on substrates is attracting a considerable interest mainly to address various challenges associated with the immobilization of preformed metal nanoparticles on different kinds of substrates. Such nanocomposite materials are important for their potential applications in optics, electronic devices, catalysis, sensors, medical applications, and so forth [24–28]. For example, antibacterial properties of polymer-supported silver nanoparticles are the subject of extensive studies for their applications in antimicrobial filters [29], tissue scaffolds, body wall repairing substances [28, 30, 31], and antibacterial packaging materials for preventing bacterial infection in foodstuffs [28, 32].

Although, there exists a deep-rooted procedure for the synthesis of nanometal oxides, this procedure is required to progress in extra-active approaches for preparation including easy preparation processes with low time of reaction. The usage of microwave supported preparation is mostly vital in this concern. It has been indicated that microwave radiation can successfully govern the size spreading in a thinner range in a shorter time of reaction than the traditional heating system owing to regular and fast heating in the nanomaterials synthesis [33–35]. Additionally, microwave irradiation offers straight heating, self-regulation of heat diffusion and transmission. The thorough microwave environment contact by reactants through the materials creation is slightly uncertain and hypothetical [33]. Nevertheless, energy transfer from the microwaves to the materials is completed through the interaction of microwave energy with water or other solvents with high dielectric constant or solvents molecules with large dipole moments [36].

The objective of this work is the in situ synthesis of nanometal oxides such as nanoiron oxide, nanocoper oxide, nanocobalt oxide, and nanomanganese oxide using microwave irradiation as an energy source and the surface morphologies of the resulting treated cotton fabrics will be investigated by scan electron microscopy coupled with EDX and the distribution of prepared metal oxide nanoparticles inside the treated fabric samples will be characterized by transition electron microscopy of cross-section area of treated cotton fabric samples. Moreover, the properties of prepared cotton fabric samples such as antibacterial activity, UV-protection, and flame retardation will be evaluated. Furthermore, the durability of metal nanoparticles inside the cotton fabric samples at different washing cycles will be examined as the bacteriostasis of the treated cotton fabric.

2. Experimental Methods

2.1. Materials

Desized, bleached, and scoured cotton fabrics (100 g/m²) were supplied by El-Mehalla for Spinning and Weaving Company, Egypt. Mercaptoacetic acid and para-toluene sulphonic acid were received from Sigma-Aldrich and were 99–99.9% pure. Cobalt chloride, manganese chloride, iron chloride, and copper chloride were obtained from Sigma-Aldrich. Wholly all substances were used as analytical grade.

2.2. Preparation of Thiol-Modified Cotton Fabrics

Cotton fabrics were padded in solution containing 10% (v/v) of mercaptoacetic acid in the presence of para-toluene sulphonic acid as a catalyst and squeezed between two rollers at 100% wet pick-up; the padded and squeezed cotton fabrics were cured at 130°C for 15 min using laboratory padder. The cured cotton fabrics were washed away with sufficient water to wash out the soluble impurities and dried out in an oven dryer at 50°C for overnight. In thiol modification of the cotton fabric, the hydroxyl groups were changed into mercaptoacetate groups as shown in Scheme 1.

Scheme 1: Thiol modification of cotton fabric using mercaptoacetic acid. Thiol modification of cellubiose (basic structural repeating unit of cellulose) is shown here for clarity.

2.3. In Situ Preparation of Metal Nanoparticles on the Thiol-Modified Cotton Fabric

Before the synthesis of nanometal oxides started, the thiol-modified cotton fabrics chelated with metal salt were prepared. This was done by dipping the modified cotton fabrics into a conical flask containing 100 mL of 1 mm FeCl₃, CuCl₂, CoCl₂, and MnCl₂ solutions separately. The flasks were shaken overnight using Bench-top Shaker TZ-A Shanghai Hengyue Medical Instruments Co. Ltd. Dipped fabric samples were squeezed at 100% wet pick-up. Modified cotton fabrics containing metal salts were then placed in the microwave oven (MARS6, CEM microwave systems CEM GmbH, Germany) operating at a power of 1800 W and frequency 2455 MHz and subjected to microwave irradiation at 100°C for 5 min. Upon microwave irradiation, the cotton fabric samples gained a color corresponding to each nanometal oxide. The fabric samples containing metal nanoparticles obtained were washed with double distilled and deionized water and then dried in an oven at 70°C for 1 h.

2.4. Characterization

The presence of the thiol group in the cotton fabric was confirmed with FT-IR spectra using FT-IR (Shimadzu Scientific Instrument FT-IR-8400S) with spectral range 4000–600 cm⁻¹. Spectra were collected with a resolution of 0.9–1.0 cm⁻¹ and given as ratio of 200 single beam scans to the same number of background scans in pure KBr. KBr was ground with the sample to fine powder. The amount of sulfur group was quantitatively estimated by means of elemental analyzer (FISON instrument model 1108). The morphology of cotton fabrics modified with mercaptoacetic acid containing metal oxide nanoparticles was considered by JOEL JXA-840-A electron probe microanalyzer-scanning electron microscope (SEM) and energy dispersive X-ray analysis (EDX). Further elemental analysis and quantification of metal oxide nanoparticles were done with EDX. The real metal oxide nanoparticles contents were determined by atomic absorption spectroscopy (Varian SpectrAA-220) with graphite furnace accessory and equipped with deuterium arc background corrector according to (APHA 2005). For this, suitable quantities of modified cotton fabric samples containing metals nanoparticles were digested in 75% concentrated H₂SO₄ at room temperature for 24 h. Moreover, modified cotton fabric samples containing metals nanoparticles were characterized using TEM images of the cross-section of prepared fabric samples [37, 38]. Limiting oxygen index (LOI) was determined according to the standard oxygen index test (ASTM D2863-95).

2.5. Antimicrobial Activity

Antibacterial properties of untreated cotton fabrics and thiol-modified cotton fabrics containing different metal nanoparticles (Fe, Cu, Co, and Mn) were quantitatively evaluated against gram-positive bacteria (Staphylococcus aureus) and gram-negative bacteria (Escherichia coli) according to the standard test method used to measure the reduction rate in the number of colonies formed [39], as follows: 1 g fabric sample was dipped into a test tube containing the bacteria culture solution in which the bacteria concentration was 2.5 × 10⁵/mL. The test tubes were shaken at 37°C for 2 h on a rotary shaker at 100 rpm and 1 : 100 dilutions of the test solutions were made. One milliliter of the diluted test solution was poured onto agar broth and incubated at 35°C for 24 h and the number of colonies in the agar broth was calculated using the following equation:

where is the number of colonies before shaking and is the number of colonies after 1 h shaking.

2.6. UV-Penetration and Protection Measurement

The capability of a fabric to block UV-radiation is assumed via the ultraviolet protection factor (UPF) values. UPF values of cotton fabric samples containing nanometal oxides were obtained using M350 UV-visible spectrophotometer (SDL/ATLAS) according to Australian-New Zealand Standard AS/NZS 4399:1996. A UPF value of 15–24 is categorized as good protection, 25–39 as very good protection, and above 40 as excellent protection against solar UV-radiation [40].

2.7. Washing Fastness

Washing fastness tests were carried out on thiol-modified cotton fabrics containing metal nanoparticles according to AATCC test method 61(2A)-1996. Separately round of laundry consisted of five washes at 38°C. The stability of the cotton fabrics containing metal nanoparticles was studied by antibacterial testing after 10, 20, and 30 washings with nonionic detergent.

3. Result and Discussion

3.1. FT-IR of Thiol-Cotton Fabrics

In thiol modification of the cotton fabric, the hydroxyl groups were changed into mercaptoacetate groups as shown in Scheme 1 which shows the degree of substitution of hydroxyl groups by mercaptoacetate groups. In addition, 2.5 wt% sulfur was detected in the thiolated cotton fabric by elemental analyzer.

These results indicated that 0.13 units of hydroxyl groups per glucose unit in the cellulose fiber were successfully converted to thiol groups. In this reaction scheme, para-toluene sulphonic acid acts as a catalyst and provides H⁺ ions for successful completion of the reaction.

FT-IR spectra of the thiolated cotton fabric are presented in Figure 1 which reveals a carbonyl (C=O) stretch corresponding to an ester group at 1730 cm⁻¹ formed by an esterification reaction of mercaptoacetic acid with hydroxyl group in cotton.

Figure 1: FT-IR spectra of (a) the unmodified cotton fabric and (b) thiol-modified cotton fabric.

3.2. Nanoparticles Formation and Characterization

The formation of nanoparticles in in situ thiol-modified cotton fabrics was based on the chelation of metal ions with chelating sites of the cotton fabrics (thiol groups), followed by microwave irradiation of the chelated metal ions, so that the Fe³⁺, Cu²⁺, Co²⁺, and Mn²⁺ chelating ions on thiol-modified cotton fabric samples oxidized by microwave irradiation into nanoiron oxide, nanocopper oxide, nanocobalt oxide, and nanomanganese oxide nanoparticles.

3.2.1. TEM Images

To approve the creation of nanoiron oxide, nanocopper oxide, nanocobalt oxide, and nanomanganese oxide into cotton fabrics, TEM analysis of cross-sectional samples was carried out for the prepared fabric samples. It is apparent from Figure 2 that nanometal oxides do not look likely to form agglomerates and are in good distribution inside the fabric owing to the steadiness of synthesized nanometal oxides, in the in situ cellulosic network. Figures 2(a) and 2(b) show that iron and copper oxide nanoparticles inside fabric samples looked like spherical particles and their diameters were 10–15 nm and 25–30 nm for nanoiron oxide and nanocopper oxide, respectively. TEM image for cross-section of fabric samples containing cobalt oxide nanoparticles is shown in Figure 2(c) which demonstrates that the nanoparticles had a nanotube-like shape. The length of the nanotube was 100–150 nm (mean length ~130 nm) and the diameter was ~58 nm. The synthesized cobalt oxide had a good nanotube structure and was stable. TEM image of cross-section of fabric samples containing manganese oxide nanoparticles formed is shown in Figure 2(d). Each particle was a linear nanorod structure with diameter of 50~55 nm and a length of 70~80 nm.

(a)

(b)

(c)

(d)

Figure 2: TEM images of cross-section of thiol cotton fabric containing (a) FeNPs, (b) CuNPs, (c) CoNPs, and (d) MnNPs.

3.2.2. SEM

The SEM images of thiol-modified fabric samples are shown in Figure 3. The pictures represent obviously the presence of metal oxide NPs, on cotton fabric. Also, Figure 3 illustrates EDX spectra analysis of sample (a) (fabric containing iron nanoparticles), sample (b) (fabric containing copper nanoparticles), sample (c) (fabric containing cobalt nanoparticles), and sample (d) (fabric containing manganese nanoparticles), respectively. EDX analysis was used to determine the composition of the samples. The strong signals from Fe, Cu, Co, and Mn atoms confirm the presence of these metals in the in situ cotton fabric samples. Sample 1 contained 33.25 wt% Fe, sample 2 contained 19.33 wt% Cu, sample 3 contained 16.7 wt% Co, and sample 4 contained 13.4 wt% Mn on the cotton fabric, respectively, representing significant loading of metal nanoparticles on the cotton fabric. This is more clearly confirmed in the analysis of the nanometal oxide contents of the fabric samples by AA spectral analysis. Metal contents in the thiol-modified cotton fabric samples were 33.2% (wt) Fe, 19.33 wt% Cu, 16.7 wt% Co, and 13.4 wt% Mn. These differences in the contents of nanometal oxides in the modified cotton fabric samples are according to the Irving-Williams series mentions on the comparative stabilities of complexes made by a metal ion. For high-spin complexes of the divalent ions of first-row transition metals, the stability constant for the complex creation follows the decreasing order: Mn(II) < Co(II) < Cu(II) < Fe(III). This order was established to hold for an extensive selection of ligands [41]. There are descriptions that are estimated commonly to describe the series. (i) The ionic radius is estimated to decrease frequently for Mn²⁺ to Fe³⁺. This is the regular periodic tendency and would explain the common increase in stability. (ii) The crystal field stabilization energy (CFSE) increases from zero for manganese(II) to a maximum at iron(III). This makes the complexes more and more stable.

(a)

(b)

(c)

(d)

Figure 3: SEM micrograph spectroscopy and EDX analysis of thiol-modified cotton fabrics containing (a) FeNPs, (b) CuNPs, (c) CoNPs, and MnNPs.

3.3. Antimicrobial Activity

All fabric samples presented high antibacterial efficiency (Table 1), with all fabrics revealing an effective antibacterial effect against S. aureus and E. coli bacteria; almost all bacteria were killed after 2 h of contact. In addition, at zero contact time (i.e., antibacterial activity of the fabric sample was investigated after 1 min contact), the fabric samples displayed the ability to destroy bacteria and because of the rapid diffusion of bacteria through the fabric samples and contact with the large amount of nanoparticles many bacteria were killed even after a few seconds. Table 1 reveals that antibacterial activity of modified cotton fabric sample loaded with nanometal oxides have the following decreasing order: iron oxide nanoparticles > copper oxide nanoparticles > cobalt oxide nanoparticles > manganese oxide nanoparticles > untreated cotton fabric and this may be attributed to the fact that [42] the fast penetration of nanoparticles into bacteria membranes depends on the particles sizes which follow the decreasing order (FeNPs < CuNPs < CoNPS < MnNPs) and the penetration of these nanoparticles is majorly due to oxidative strain and reasons of distraction of the cell membrane. The antibacterial medications and antibiotics improve oxidative strain by making reactive oxygen species [43]. Reactive oxygen species (ROS) can cause destruction of proteins and DNA in bacteria [44]. This bactericidal asset has been demonstrated from silver metals by Park et al. [45]. So the higher antibacterial action of FeNPs toward the S. aureus and E. coli could be owing to the synergistic influence of the phytochemicals, magnetic properties, and proliferation of ROS by FeNPs more than the other nanoparticles.

Table 1: Antibacterial activity of thiol-modified cotton fabrics containing different nanometal oxides.

Fabric sample containing
Antibacterial efficiency (%)

Exposure time (0 hr)
Exposure time (2 hr)
Exposure time (0 hr)
Exposure time (2 hr)

S. aureus
S. aureus
E. coli
E. coli

Untreated cotton fabric
—
—
—
—

FeNPs
99.9
100
98.8
100

CuNPs
99.7
100
98.5
100

CoNPs
98.8
100
96.9
98.7

MnNPs
97.9
100
95.0
97.8

3.4. UV-Penetration and Protection Measurement

To evaluate the potential effectiveness of certain metal nanoparticles chemically bonded to the in situ cotton fabrics samples for enhancing the UPF values, four metal nanoparticles such as FeNPs, CuNPs, CoNPs, and MnNPs were used for their capability to elevate the intrinsic UV-blocking toward the injurious UV-irradiation. Results of Table 2 indicate that (i) the presence of metal nanoparticles inside the cotton fabric samples increases the UPF values compared to the cotton fabrics without treatment, (ii) the degree of upgrading in the UV-protection properties is estimated via the kind of metal nanoparticles and follows the order: FeNPs > CuNPs > CoNPs > MnNPs > cotton fabric without nanometal oxide, and (iii) the difference in the UV-protecting command of prepared fabric samples in existence of the abovementioned nanometal oxides reveals the variances between them in molecular weight, content, particle size, surface area, position and the degree of spreading, obstructive assets, and absorbing capability [46].

Table 2: Effect of metal nanoparticles type and content on UV protective factor properties of prepared fabrics.

Metal type
Metal content
UPF rating

Untreated cotton fabric
0
6.0

Cotton fabric-FeNPs
33.25 wt%
>50

Cotton fabric-CuNPs
19.33 wt%
50

Cotton fabric-CoNPs
16.74 wt%
45

Cotton fabric-MnNPs
13.37 wt%
40

3.5. Flame Retardation

The limited oxygen index (LOI) values of prepared fabric are obtained in Table 3. The data so found display that the prepared fabric samples containing metal oxide nanoparticles have LOI values of 45.12, 30.8, 26.4, and 24.8, for fabric samples containing FeNPs, CuNPs, CoNPs, and MnNPs, respectively. This may be due to the capability of these nanoparticles to form blocks that prevent Linder-directed contact with heat. The LOI value of FeNPs is greater than that of the other nanoparticles. This is due to the fact that the FeNPs have a good capability to absorb heat in comparison to the particle under investigation. LOI values of prepared fabric samples containing metal oxide nanoparticles follow a decreasing order: cotton fabric sample containing FeNPs > cotton fabric sample containing CuNPs > cotton fabric sample containing CoNPs > cotton fabric sample containing MnNPs > untreated cotton fabric sample.

Table 3: LOI values of the prepared cotton fabric samples containing different metal oxide nanoparticles.

Nanoparticles
LOI value

Untreated cotton fabric
15.6

Cotton fabric-FeNPs
45.12

Cotton fabric-CuNPs
30.8

Cotton fabric-CoNPs
26.4

Cotton fabric-MnNPs
24.8

3.6. Washing Fastness of Antibacterial Property

The washing fastness of sample 1 (fabric containing iron nanoparticles), sample 2 (fabric containing copper nanoparticles), sample 3 (fabric containing cobalt nanoparticles), and sample 4 (fabric containing manganese nanoparticles) was assessed under different conditions including no washes and 10, 20, and 30 washes. The antibacterial action of each sample against gram-positive and gram-negative bacteria was investigated after 2 h exposure based on the AATCC 61(2A)-1996 washing test method. Table 4 shows that the antibacterial activity of fabric samples at 0 washing cycles was slightly greater than that of the fabric samples at the other washing cycles and this is attributed to the presence of slight amount of nonchelated nanoparticles which are present on the surface of the cotton fabrics. Repeating washing cycles (10 to 30) had no decreasing effect on the antibacterial activity due to nanoparticles which are not leached out the fabric samples because there is a chemical reaction between thiol groups on cotton fabrics and the nanoparticles. Therefore, by these chemical bonds, the nanoparticles did not leach out the fabrics by washing even after 30 laundering washing cycles, indicating excellent durability of the nanotreatment fabric samples against washing.

Table 4: Antibacterial reduction percentages of cotton samples containing metals nanoparticles: sample 1 (FeNPs), sample 2 (CuNPs), sample 3 (CoNPS), and sample 4 (MnNPs).

Number of washings
Sample 1
Sample 2
Sample 3
Sample 4

S. aureus
E. coli
S. aureus
E. coli
S. aureus
E. coli
S. aureus
E. coli

0
100
100
100
100
100
98.7
100
97.8

10
99.5
97.8
98.4
96.6
97.3
96.5
98.6
95.3

20
99.5
97.8
98.4
96.6
97.3
96.5
98.6
95.3

30
99.5
97.8
98.4
96.6
97.3
96.5
98.6
95.3

4. Conclusion

Multifunctional properties of cotton fabrics such as antibacterial activity, UV-protection, and flame retardation were developed by in situ preparation of nanometal oxides such as iron oxide, copper oxide, cobalt oxide, and manganese oxide nanoparticles into thiol-modified cotton fabric using microwave irradiation. Firstly, thiol-modified cotton fabrics were prepared by the treatment of cotton fabrics with mercaptoacetic acid in the presence of para-toluene sulphonic acid as a catalyst. Furthermore, in situ preparation of aforementioned nanometal oxides into modified cotton fabrics was obtained. Formed nanoparticles do not seem to form coagulates and were well spread inside the fabric; iron and copper oxide nanoparticles inside fabric samples looked like spherical particles and their diameters were 10–15 nm and 25–30 nm for iron oxide nanoparticles and copper oxide nanoparticles, respectively, while cobalt oxide nanoparticles had a nanotube-like shape. The length of the nanotube was 100–150 nm and the diameter was ~58 nm and manganese oxide nanoparticles formed had a linear nanorod structure with a diameter of 50~55 nm and a length of 70~80 nm.

All prepared cotton fabric samples containing these nanometal oxides have high antibacterial efficiency, with an effective antibacterial effect against S. aureus and E. coli bacteria; almost all bacteria were killed after 2 h of contact. In addition, at zero contact time, the fabric samples displayed the ability of destroying bacteria. Antibacterial activities of modified cotton fabric samples loaded with nanometal oxides have the following decreasing order: iron oxide nanoparticles > copper oxide nanoparticles > cobalt oxide nanoparticles > manganese oxide nanoparticles > untreated cotton fabric.

The presence of the aforementioned nanometal oxides nanoparticles inside the cotton fabric samples increases the UPF values and the degree of enhancement in the UV-blocking properties is estimated by the kind of metal nanoparticles and follows the following order: FeNPs > CuNPs > CoNPs > MnNPs.

Prepared fabric samples containing metal oxide nanoparticles have LOI values of 45.12, 30.8, 26.4, and 24.8, for fabric samples containing FeNPs, CuNPs, CoNPs, and MnNPs, respectively. LOI values of prepared fabric samples containing metal oxide nanoparticles follow a decreasing order: cotton fabric sample containing FeNPs > cotton fabric sample containing CuNPs > cotton fabric sample containing CoNPs > cotton fabric sample containing MnNPs > untreated cotton fabric sample.

The covalent bonds between the unhydroglucose unit of cotton fabrics and these nanometal oxides were imparted strong antibacterial action to the modified substrates against gram-positive (S. aureus) and gram-negative (E. coli) bacteria strains even after 30 successive home washing rounds.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Acknowledgment

The authors are grateful to the Dean of Scientific Research at King Faisal University for their financial support for this project work through project no. 130190.

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