The Core/Shell Structure of CdSe/ZnS Quantum Dots Characterized by X-Ray Absorption Fine Spectroscopy

Understanding the chemical and physical properties of core/shell nanocrystal quantum dots (QDs) is key for their use in lightemission applications. In this paper, a single-step injection-free scalable synthetic method is applied to prepare high-quality core/shell QDs with emission wavelengths of 544 nm, 601 nm, and 634 nm. X-ray absorption fine structure spectra are used to determine the core/shell structure of CdSe/ZnS quantum dots. Moreover, theoretical XANES spectra calculated by FEFF.8.20 are used to determine the structure of Se and S compounds. The QD samples displayed nearly spherical shapes with diameters of approximately 3.4± 0.5 nm (634 nm), 4.5± 0.4 nm (601 nm), and 5.5± 0.5 nm (544 nm). With XANES results andMS calculations, it is indicated that sphalerite ZnS capped with organic sulfur ligands should be the shell structure. Wurtzite CdSe is the main core structure with a Cd-Se bond length of 2.3 Å without phase shift. This means that different emission wavelengths are only due to the crystal size with single-step injection-free synthesis.Therefore, single-step injection-free synthesis could generate a nearly ideal core/shell structure of CdSe/ZnS QDs capped with an organic sulfur ligand.


Introduction
Over the last 30 years, a significant amount of research has been reported regarding quantum dot (QD) synthesis.The fundamental physical properties and potential applications of quantum dots include multiplexed labeling and tracking of cells or molecules in a biological environment, downshifting light for color engineering in solid-state lighting, illumination and displays, and single-photon sources.Among various semiconductor materials, CdSe has been most extensively studied because its size-dependent photoluminescence is tunable across the visible spectrum [1][2][3][4][5], but it is insufficiently stable and is sensitive to the processing conditions and the environment [6,7].To address these disadvantages, scientists have focused on the development of core/shell QDs, which incorporate a shell of wider band gap semiconductor materials around the QD cores [8][9][10][11][12][13].These core/shell QDs have been shown to be generally more robust against chemical degradation and photooxidation [14][15][16][17].
Knowledge of the chemical and physical properties of semiconductor nanocrystals is a key issue in understanding the mechanisms of the dots formation and their position correlations.Many techniques have been used to determine information on their structural properties and ordering phenomena [18][19][20][21].Element sensitive transmission electron microscopy (TEM) studies have provided information about the CdSe core structure and the surrounding ZnSe shells [22].The CdSe/ZnS core/shell structure was elucidated using high-resolution TEM/STEM [20,23].The structure of core/shell/shell CdSe/ZnSe/ZnS QDs was also studied using photoluminescence spectroscopy [24].Recently, the structural evolution of the interfaces in CdSe/CdS and CdSe/Cd 0.5 Zn 0.5 S colloidal QDs was investigated using surface-enhanced Raman spectroscopy [25].However, not all of these techniques can reveal structural properties.Because of the small scattering volume of CdSe/ZnS QDs, it is not possible to obtain any information using high-resolution Xray diffraction (HRXRD).Therefore, the understanding of the local structure of these QDs and their environments is still rudimentary.
X-ray absorption near edge structure (XANES) [26][27][28] and extended X-ray absorption fine structure (EXAFS) [29,30] are sensitive to the oxidation state/site symmetry of elements and to the local structure around a selected atom.Recently, the core/shell structure of CdSe x S 1−x QDs was explored using XANES and EXAFS [31].The local structure of CdSe/ZnSe QDs was analyzed using EXAFS and diffraction anomalous fine structure (DAFS).XANES is largely a fingerprint for the oxidation state and site symmetry of an element.Meanwhile, theoretical XANES spectra were calculated using the ab initio multiple-scattering code FEFF8.20 [32,33].Comparing the theoretical spectra with the experimental spectra can reveal detailed structural information about QDs.Therefore, we use both XAFS experimental data and XANES theoretical calculations to probe the fine structure of QDs obtained from a single-step injection-free synthesis.
In the previous work [12], we developed a single-step injection-free synthesis of high-quality core/shell QDs.We considered the QDs structure to be CdSe/Zn x Cd 1−x S.However, it is difficult to collect detailed structural information using conventional test methods.In this paper, the structural information of QDs is confirmed using EXAFS and XANES with MS calculations.
QDs with an emission wavelength of 544 nm were obtained by adding 0.3 mL TOP and 1.14 g (4 mmol) SA.QDs with an emission wavelength of 601 nm were obtained by adding 0.4 mL TOP and 1.71 g (6 mmol) SA.QDs with an emission wavelength of 534 nm were obtained by adding 3.0 mL TOP and 0.99 g (3.5 mmol) SA and substituting Zn(NO 3 ) 2 with Zn(OAc) 2 .
X-ray absorption fine spectroscopy (XAFS) data for the Se and Cd K-edge were collected on Beamline BL14W1 at the Shanghai Synchrotron Radiation Facility (SSRF).The incident radiation was filtered using a Si (311) doublecrystal monochromator with a suppression mirror system for focusing and harmonic rejection.All data were taken in transmission mode using an Oxford ion gas chamber.The Se K-edge spectra were calibrated by defining the zero crossing point of the second derivative of the XANES spectra for the Se reference foils as 12658 eV, and the Cd K-edge spectra were calibrated by defining the zero crossing point of the second derivative of the XANES spectra for the Cd reference foil as 26711 eV.The S K-edge X-ray absorption near edge structure (XANES) spectra were collected on Beamline 4B7A at the Beijing Synchronization Radiation Facility (BSRF).The sulfur K-edge spectrum of the QDs was obtained using a double-crystal monochromator (DCM) and recorded in Xray fluorescence yield (XFY) mode.The XAFS and XANES data were analyzed using the IFEFFIT software [34], and the cluster size dependence was determined using the ab initio multiple-scattering code FEFF8.20 [32,33].

Results and Discussion
3.1.Optical Properties.The nanocrystal quantum dots (QDs) were synthesized using the single-step injection-free approach, as shown in Figure 1(a), which produced highquality core/shell QDs [12].The detailed procedures are described in the materials and methods.Briefly, CdO, Zn(NO 3 ) 2 , Se, and S were combined in an ODE solution con-taining TOP and SA at room temperature and then heated to 250 ∘ C under stirring.As shown in Figures 1(b)-1(c), the three samples have different colors under a UV lamp with emission wavelengths of 544 nm (green), 601 nm (yellow), and 634 nm (red).The symmetric and narrow band edge PL emission peak in the PL spectra indicates that the particle sizes and shapes are nearly uniform [12].

Morphology of Obtained
QDs.The characterization of QD samples with three different emission wavelengths was performed using TEM and HRTEM, as shown in Figure 2. At different growth stages, the QD samples displayed nearly spherical shapes with diameters of approximately 3.4±0.5 nm (634 nm), 4.5 ± 0.4 nm (601 nm), and 5.5 ± 0.5 nm (544 nm).

Structural Characterization of the QDs.
To understand the relationship between the structure and the variation in the optical properties of the three QDs samples, X-ray absorption spectroscopy (XAS) was employed.X-ray absorption near edge structure (XANES) and extended X-ray absorption fine structure (EXAFS) data reduction was conducted using standard procedures [35].XANES is largely a fingerprint for the oxidation state and site symmetry of the elements.
The S K-edge XANES spectra were collected to determine the S compounds in the QD samples.Figure 3(a) shows the S K-edge XANES spectra of the three QDs with emission wavelengths of 544 nm, 601 nm, and 634 nm.Two strong peaks are observed in the S K-edge spectra of the three samples.The first peak (A) is at approximately 2472 eV with two small shoulder peaks (2474 eV and 2476 eV), and the second peak (D) is at approximately 2482 eV.This strong peak, indicated by the white line, arises from the 1s to 3p transition.The white-line energy shift is closely related to the oxidation state of sulfur.The energy shift of the white line in the S K-edge XANES spectra shows a nearly linear dependence on the oxidation state and varies by approximately 12.0 eV from S −2 to S +6 [36].Thus, the first peak (A) in our samples might incorporate contributions from S −2 , while the second peak (D) comes from S +4 or S +6 .The possible sulfur compounds in S −2 are ZnS, CdS, and Zn x Cd 1−x S, but it is difficult to distinguish these three sulfur compounds in the S K-edge XANES spectra.Additionally, organic sulfur ligands with sulfur in the S +4 or S +6 state cap the QDs.
To distinguish the structure of S in the obtained QD samples, we performed MS calculations of the S K-edge XANES in sphalerite ZnS (Figure 3(b)) and CdS (Figure 3(c)) using different clusters [37].For ZnS, using a central sulfur atom, the first shell contains only four coordinated zinc atoms and makes only a small contribution to the features of the spectrum.The 17-atom cluster gives rise to the whiteline peak A, demonstrating the influence of the relatively strong backscattering of the S −2 ions [38].Features B and C were reproduced using clusters larger than 99 atoms, which include atoms up to 6 Á from the central sulfur atom, indicating that these peaks arise from "medium-range" contributions.There is a white peak A and a shoulder peak B/C in the S K-edge XANES spectra, which indicates that ZnS should be a major component of the samples.The zinc-blend structure of CdS is stable at room temperature but transforms to the wurtzite phase above 95 ± 5 ∘ C [31].Therefore, we performed MS calculations of the S K-edge XANES in the wurtzite phase of CdS.For CdS, with a central sulfur atom, the first shell contains only four coordinated cadmium atoms and only makes a small contribution to the features of the spectrum.Prepeak A, the white-line peak B, and shoulder peak C were produced in clusters larger than 92 atoms.Therefore, no prepeak A or shoulder peak C was observed in the S K-edge XANES spectra of the QD samples.Additionally, the white-line peak in CdS is in almost the same position as ZnS.Thus, we suggest that no CdS compounds exist in the obtained samples.
The Cd K-edge XANES spectra were also collected to determine the Cd compounds in the QD samples.Figure 4 shows the Fourier transform (FT) magnitudes of the  2weighted (k) function using a Kaiser-Bessel window and the k-range of 2.8-10.5 for all samples.There are two peaks at approximately 1.7 Å and 2.3 Å without a phase shift.Based on previous results [21,31,[39][40][41], the Cd-Se bond has a peak between 2.0 and 3.2 Å in the Cd K-edge RSF function.In our results, there is a peak at approximately 2.3 Å without a phase shift, which was confirmed to be from the Cd-Se bond.Furthermore, there is a strong peak at approximately 1.7 Å without a phase shift, which can be ascribed to the Cd-O bond of TOPO-Cd [42].In the case of the Cd : Se = 4 : 1 system, the extra Cd atoms cap the CdSe with TOPO attached to both free Cd and CdSe.Meanwhile, as the concentrations of TOP and SA increase, the Cd-O peak intensity decreases gradually, and the Cd-Se peak intensity simultaneously increases, which implies that higher TOP and SA concentrations facilitate the conversion of TOPO-Cd into CdSe.Based on the above results, the CdSe is considered the core of the obtained QDs and has a wurtzite structure.The Cd atoms are located at the tetrahedral sites of a face-centered cubic structure that consists of Se atoms.
The white lines and intensities in the Se K-edge XANES spectra of the QD samples (Figure 5(a)) are similar, indicating that the Se compounds in the three samples are nearly identical.With previous work [8,[43][44][45], we considered that CdSe was the only compound for element Se in the QD samples.To distinguish the scattering contributions from the different coordination shells of CdSe, which has a wurtzite structure above 95 ± 5 ∘ C [46], MS calculations of the Se Kedge XANES spectra were performed using different clusters, as shown in Figure 5(b).The results indicate that the XANES spectra for the 5-atom and 17-atom clusters with one and two shells are significantly different from the 29-atom cluster and larger clusters with more than three shells.Compared with the XANES spectra of three samples, the results indicate that the QDs samples have more than 47 atoms, and the small clusters are in wurtzite structures.
Three QD samples with different emission wavelengths were obtained using the single-step injection-free synthesis.CdSe compounds with the narrowest band gap of 2.25 eV [47,48] exist in the center of the QD samples as the core structures.Meanwhile, ZnS compounds comprised the shell structures.Furthermore, the CdSe core structure has a wurtzite structure with a Cd-Se bond length of 2.3 Å without phase shift, and the ZnS has a sphalerite structure.The core structure and shell structure are almost identical for the three different QD samples, which might be caused by the reaction stages.The single-step injection-free synthesis method combines all of the reactants in an ODE solution, which is then heated to 250 ∘ C with stirring.When the temperature is greater than 95 ± 5 ∘ C, CdSe exists only in the wurtzite phase.Based on this synthetic method, it can be concluded that the change in the QD emission wavelength is only caused by the size change due to the TOP and SA concentrations.Therefore, the single-step injection-free synthesis can generate a nearly ideal core/shell structure of CdSe/ZnS QDs capped with sulfur organic ligands, and it can tune the emission wavelength from violet to near infrared.

Conclusions
A single-step injection-free scalable synthetic method was used to prepare core/shell QDs with different emission wavelengths.The core/shell structure of the QDs with three different emission wavelengths (544 nm, 601 nm, and 644 nm) was performed using EXAFS and XANES with MS calculations.The structure of the CdSe (core) and ZnS (shell) in the three QD samples is nearly identical.The XAFS results show that CdSe is the main core component with a wurtzite structure and a Cd-Se bond length of 2.3 Å without phase shift.They also indicate that the ZnS has a sphalerite structure, and the shell structure is capped with organic sulfur ligands with sulfur in the S +4 or S +6 state.Based on this synthetic method, it could be concluded that the changes in the QD emission wavelengths are only caused by the size changes of the nanocrystal, due to the TOP and SA concentrations.Therefore, this single-step injection-free synthesis could generate nearly ideal core/shell CdSe/ZnS QDs capped with organic sulfur ligands.

Figure 1 :
Figure 1: (a) Overall scheme for the single-step noninjection synthesis of QDs.(b) Photographs of typical emission color under the UV lamp.(c) PL emission spectra of obtained QDs with emission wavelengths of 544 nm (A), 601 nm (B), and 634 (C).

Figure 4 :Figure 5 :
Figure 4: The magnitude of the Fourier transform (FT) of the  2 -weighted experimental EXAFS data of three obtained QDs with emission wavelengths of 544 nm (green), 601 nm (yellow), and 634 nm (red).