Evaluation of Antioxidant Ability In Vitro and Bioavailability of trans-Cinnamic Acid Nanoparticle by Liquid Antisolvent Precipitate

TCD is a kind of organic acid that is isolated from cinnamon bark or benzoin. TCD has significant antioxidant activity and is widely used in pharmaceutical, cosmetic, and food additives. But TCD has shortcomings of low bioavailability due to poor water solubility.Therefore, we use ethanol as a solvent, deionized water as antisolvent, and hydroxypropyl methylcellulose (HPMC) as the surfactant to prepare TCD nanoparticle powder. The optimum preparation conditions were determined as follows: TCD-ethanol solution concentration was 170mg/mL, the volume ratio of antisolvent was 4 times that of solvent, and the amount of the surfactant was 0.3% stirred for 10min by 2500 rpm; TCD nanoparticle with a mean particle size (MPS) of 130±12.5 nm is obtained under the optimum conditions. SEM, FT-IR, LC-MS/MS, XRD, andDSCwere used to characterize the TCD nanoparticle.The results showed that the chemical structure of TCD nanoparticle was not changed, but the crystallization was significantly reduced. Solubility, dissolution rate, antioxidant activity, the in vitro transdermal penetration, and bioavailability of TCD nanoparticles were all much better than these of the raw TCD.These results suggested that TCD nanoparticle might have potential value to become a new oral or transdermal TCD formulation with high bioavailability.


Introduction
trans-Cinnamic acid (TCD, also known as trans-3-phenylacrylic acid) is one of the main chemical constituents of cinnamon which belongs to plant Lauraceae [1,2].This acid possesses antibacterial, elevated white blood cell, cholagogue, and anticancer effects [3].It can also inhibit melanoma and proliferation and promote differentiation of cancer cell in vitro [4,5].It may be indirectly combined with DNA to regulate the expression of certain genes and block the growth and proliferation of tumor cells without embryotoxicity performance for clinical treatment of tuberculosis [6].TCD as a natural oral antioxidant has a significant antioxidant effect and plays an important role in clearing oxygen-free radicals of the body, delaying human aging, and improving immunity [7].However, the application of TCD is restricted by its poor water solubility and low oral bioavailability.
Preparation of nanoparticles with small and uniform particle size distribution is an effective way to improve the bioavailability of poorly soluble drugs [7].Preparation technology of nanoparticles mainly includes ball milling method, jet milling method, supercritical fluid technology, and liquid antisolvent precipitate.Mechanical pulverization methods, such as ball milling and jet milling, are widely used to prepare nanoparticle drugs, but their disadvantages include energy consumption, low efficiency, and a wide particle size distribution [8].Supercritical fluid technology has several disadvantages, such as large investment in equipment, low yield, and difficulty for industrialization [9].Liquid antisolvent precipitate is an important way to prepare drug nanoparticles [10].It has potential prospects because of its low cost and easy operation compared with traditional preparation technology [11].To date, numerous types of poorly soluble oral drugs have been successfully prepared by this method [12].
In the present work, we prepared TCD nanoparticle using liquid antisolvent precipitate, which has not been reported in literature.This work aimed to prepare the TCD nanoparticle to improve dissolution rate, oral antioxidant, and bioavailability.Scanning electron microscope (SEM), Fourier transform infrared (FT-IR), spectroscopy liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), Xray diffraction (XRD), and differential scanning calorimetry (DSC) were used to characterize TCD nanoparticle.Solubility, dissolution rate, purity, transdermal penetration, antioxidant activity, and bioavailability of TCD nanoparticle were also evaluated.

Preparation of TCD Nanoparticle.
Ethanol and deionized water were selected as solvent and antisolvent, respectively, to prepare TCD nanoparticles during liquid antisolvent precipitate.The experimental processes are illustrated in Figure 1.At room temperature, the raw TCD-ethanol solution was injected into the antisolvent with 0.3% (w/w) HPMC by an injection pump (JZB-1800D; Jianyuan Medical Instrument, China) at an injection rate of 1 mL/min stirring at a certain speed intensity, which resulted in a rapid formation of milky suspension.A small amount of suspension was then collected and diluted.Subsequently, a laser particle size analyzer was used to detect the mean particle size (MPS).The remaining suspension was centrifuged for 10 min under the condition of 10000 rpm; then residual ethanol was removed using deionized water by three times of washing and the precipitation was collected.30% mannitol was added to the suspension as freeze-dried cryoprotector.Finally, TCD nanoparticles were obtained by prefreezing for 12 h at −40 ∘ C in a refrigerator and then lyophilized at −50 ∘ C for 48 h.TCD nanoparticles were without mannitol under same freezedrying conditions.

Optimization of TCD Nanoparticle.
Single-factor analysis was employed to optimize the TCD nanoparticle operating conditions by liquid antisolvent precipitate process.Through preliminary experiments, we selected the following five factors which had an arresting impact on the MPS of the TCD nanoparticle: precipitation time at 3-30 min, concentration of TCD-ethanol solution at 30-170 mg/mL, volume ratio of antisolvent to solvent at 1 : 1-8 : 1, amount of surfactant at 0.1%-0.5%,and stirring intensity at 500-2500 rpm.The detailed level of each factor is presented in Table 1.A laser diffraction particle size analyzer was used to detect the MPS of the TCD nanoparticle and determine optimized conditions of the antisolvent precipitation process results.The optimum condition for every factor was selected based on the smallest MPS.

Analysis of Morphology and Particle
Diameter.The morphologies of the TCD powders were detected by SEM (Quanta 200; FEI, Netherlands).The samples were prepared by direct deposition of the powder onto a carbon tape placed on the surface of an aluminum tray and were coated with gold for 4 min using a sputter coater before analysis.The MPS of TCD nanosuspension was detected by DLS (ZetaPALS; Brookhaven Instruments, USA).TCD nanosuspension sample (3 mL) was added to the sample cell to detect the MPS.The sample was diluted with a mixture with the same composition as that during the antisolvent process if necessary.Each measurement was repeated at least three times.

FT-IR.
FT-IR spectrum of the samples was obtained by IRAffinity-1 (Shimadzu, Japan) and recorded in the wavenumber range of 4000 cm −1 to 500 cm −1 at a resolution of 4 cm −1 .The raw TCD powders were diluted with KBr mixing powders at 1% and separately pressed to self-supporting disks [13,14].

LC-MS/MS.
The quantitative analyses of the raw TCD and TCD nanoparticles were monitored using a triple quadrupole mass spectrometer (API300, MDS Sciex, USA) equipped with an APCI interface [15].The samples were dissolved in methanol.The mass spectrometer was operated in electrospray ionization (ESI) negative ion mode with the ion spray voltage 5500 V.The other parameters were as follows: collision gas was at 6 L/min, the curtain gas was at 10 L/min, and the nebulizing gas was at 12 L/min./ ranged from 100 amu to 1000 amu.

XRD.
The crystal forms of the samples were detected using an X-ray diffractometer (Philips, Xpert-Pro; Netherlands).The current and voltage using Cu Kl radiation were 30 mA and 40 kV, respectively.The angular range was scanned from 5 ∘ to 50 ∘ of 2, with a step size of 0.02 ∘ at a rate of 5 ∘ /min [16].
2.2.5.DSC.Thermal analysis was carried out using DSC (TA instruments, model DSC 204) for raw TCD and TCD nanoparticle.Analysis was performed for 5.0 mg samples at temperature from 35 to 200 ∘ C at a heating rate of 10 ∘ C/min under N 2 atmosphere.

GC.
The residual amounts of ethanol in the TCD nanoparticle were analyzed using an Agilent 7890A gas chromatograph (Agilent Technologies, Palo Alto, CA, USA) with an HP-5 5% phenyl methyl siloxane capillary column (30.0 m × 320 mm × 0.25 m, nominal) equipped with a G1540N-210 flame ionization detector.TCD nanoparticle (0.2 g) was weighed and placed into a 10 mL volumetric flask, and 10 mL of ethanol was added.The flask was shaken by ultrasonic agitation at 20 ∘ C. The sample was then centrifuged at 10000 rpm for 10 min, and 1.0 L of supernatants was injected into the GC injection port.The GC analysis conditions of ethanol were as follows.The oven temperature was initially maintained at 40 ∘ C for 12 min, raised at a rate of 10 ∘ C/min to 240 ∘ C, and held for 10 min.The injector temperature was 200 ∘ C, and the detector temperature was 280 ∘ C. Nitrogen gas was used as carrier gas at a flow rate of 25 mL/min, and 5 L samples were manually injected in split mode with a split ratio of 20 : 1. Hydrogen gas and air flow rates were 30 and 400 mL/min, respectively.
2.2.7.HPLC Condition.HPLC was analyzed with a Waters chromatograph (Waters Co., USA).The chromatographic column was Diamonsil C 18 reverse-phase column (250 mm × 4.6 mm, 5 m; China).The mobile phase consisted of 30 : 70 (v/v) mixtures of acetonitrile and 0.1% phosphoric acid solution.The column temperature was set at room temperature.The injection volume and flow rate were 20 L and 1.0 mL/min, respectively.The effluent was monitored at 278 nm.

Surfactant Residual Analysis.
The method for determination of the amount of HPMC in TCD nanoparticles was established in this part as follows: 3 mL of 0.5 mg/mL TCD nanoparticles water solution and 5 mL diphenylamine solution (1.4% diphenylamine, 39.7% glacial acetic acid, and 58.9% concentrated hydrochloric acid) (w/w) were added to the comparison tubes.After mixing, the comparison tubes were heated in a boiling water bath for 30 min and finally cooled to room temperature in ice bath [17].The absorbance spectrum was scanned using a 5500PC ultraviolet spectrophotometer (Yuanxi Instruments, China), at wavelengths from 500 to 700 nm.The HPMC comparisons were tested and content was calculated using a standard curve based on absorbance at 634 nm; then the content of HPMC which remained could be determined.

Dissolution Rate Test.
The dissolution rate of the TCD powders was analyzed.The paddle speed and solution temperature were set at 75 rpm and 37.0 ± 0.5 ∘ C, respectively.Artificial gastric juice (pH 1.5) with 0.5% (w/w) Tween-80 was used as dissolution medium.The raw TCD (5 mg) and physical mixture of raw TCD with HPMC and mannitol (containing 5 mg TCD) and TCD nanoparticles (containing 5 mg TCD) were added to the dialysis bag with 200 mL artificial gastric juice as dissolution medium.Samples (5 mL) were withdrawn each time at 5, 10, 20, 30, 45, 60, 90, and 120 min.The same volume dissolution medium was then supplemented.The filtrate was directly injected into an HPLC system and assayed for TCD concentration.

DPPH Free Radical Scavenging.
The DPPH radical scavenging assay is commonly employed to evaluate the antioxidant abilities to scavenge free radicals [18].Using the DPPH free radical method is advantageous in evaluating antioxidant effectiveness because it is more stable than the hydroxyl and superoxide radicals [19].The antioxidant of the raw TCD and TCD nanoparticles was determined by DPPH radical scavenging assay by UV spectrophotometry.The raw and TCD nanoparticle were dissolved at a certain concentration with deionized water, mixed by ultrasound shaking for 1 h, and centrifuged.Different concentrations of 1.5 mL sample solution were added to 1.5 mL 0.1 mol/L DPPH in 95% ethanol.The mixture was mixed, vibrated, and placed at room temperature for 30 min.Then the absorbance of the samples was tested at a wavelength of 517 nm.The clearance rate was calculated as follows: clearance rate (%) = (( 0 − )/ 0 ) * 100, where  0 is the absorbance of blank and  is the absorbance of sample; 1.5 mL of distilled water added to 1.5 mL of 95% ethanol to zero is blank [20].

In Vitro Transdermal Penetration.
A transdermal diffusion device (Henan Instrument Co., Ltd., China) was used to analyze the in vitro transdermal penetration by using the improved Franz diffusion cell method [21].The receiving pool volume was 5 mL, and the effective area was 0.95 cm 2 .The Sprague-Dawley (SD) rat (Animal Ethics Committee of Harbin Medical University) weighed approximately 200 g.A blade was used to shave off abdominal hair after the rat was sacrificed.The abdominal skin was cut, and the subcutaneous fat layer and the connective tissue were peeled off and cleaned with saline water.The abdominal skin of SD rat was punched into two same round pills.The cut layer of the rat skin was placed facing the two supply rooms and fixed in the middle of the diffusion pool.The TCD nanosuspension and raw drug suspension (5 mL) were added to the two supply rooms with a concentration of 1 mg/mL.PBS (5 mL) was later added to both receiving rooms.The dermis and receiving liquid were allowed to contact completely.The diffusion pool was maintained at 37 ± 0.5 ∘ C and stirred at 100 rpm.Samples (1 mL) were tested at 0.5, 1, 1.5, 2, 3, 4, 5, and 6 h after the 30 min balance time; meanwhile, the same amount of PBS was added.The samples were processed by centrifugation, and the supernatant was obtained and detected by HPLC at least three times.The in vitro cumulative penetration amount of per unit area was calculated by the following formula: where   is the drug concentration of  ( = 1, 2, 3, 4, 5, 6, 7, and 8) sampling point,   is the drug concentration of  ( =  − 1) sampling point,   is the volume of receiving pool size (5 mL), and   is the sample volume (1 mL). is the quantity of reagent [22].

Animals and Sample Preparation.
Twelve male Sprague-Dawley (SD) rats weighing 200 g to 250 g were used in the study.The rats were randomly divided into two groups of six.The rats were housed under standard conditions of temperature, humidity, and light with food and water provided ad libitum.They were allowed to acclimatize in the laboratory for at least 1 week prior to the experiment.Before administration, the animals were fasted overnight with free access to water and were given ether anesthesia before the test.The raw TCD (15 mg) and TCD nanoparticles (containing 15 mg TCD), which contain 30% mannitol and 2.25% HPMC, were dispersed in 3 mL of deionized water.Two groups of male rats ( = 6) were administered with an oral dose (20 mg/kg by gavage).Heparin (50 mg) was dissolved in 5 mL of deionized water, shaken, and mixed by ultrasound shaking.Blood samples obtained by puncture of the orbital venous sinus were collected into heparinized tubes before and at 0.08, 0.25, 0.5, 1, 1.5, 2, 2.5, 3, 5, 8, 12, and 24 h after oral administration.The samples were immediately centrifuged at 3000 rpm for 10 min, and aliquots of plasma were stored at −40 ∘ C until additional extraction and analysis.

Preparation of Plasma Samples.
Frozen samples were thawed at room temperature and treated as follows.Each 300 L of aliquot of plasma sample was diluted by 900 L of ethanol, shaken for 2 min, and centrifuged at 10000 rpm for 10 min.The supernatant solution was directly injected into the HPLC system.The analysis conditions were the same as described above.The concentration of the drug was calculated according to the peak area and standard cure.The oral bioavailability of the TCD nanoparticle was calculated by the area under the plasma concentration-time curve (AUC).

Impact of Various Factors on the MPS of TCD Nanosuspension and Optimization Study.
The optimization experiments were carried out separately at 1, 3, 5, 10, 20, and 30 min.
Other conditions, such as the volume ratio of antisolvent to solvent, the amount of surfactant, the concentration of TCD-ethanol solution, and the stirring intensity, were maintained at 4 : 1, 0.3%, 150 mg/mL, and 1000 rpm, respectively.Figure 2(a) shows that no notable change occurred on the MPS of the TCD nanoparticle.The supersaturation of the TCD nanoparticle within 0 min to 10 min became larger because an antisolvent was added to the solvent during liquid antisolvent precipitate process.Therefore, the MPS within 0 min to 10 min was relatively large.By contrast, within 10 min to 30 min, the nanoparticle was stabilized.
The supersaturation of the TCD solution increased to the maximum value with longer stirring time and then remained unchanged.This induced that the MPS was reduced and finally maintained.Therefore, the optimal precipitation time of the TCD nanoparticle was determined at 10 min.The concentration of TCD-ethanol solution has a significant impact on the MPS.The optimization experiments were carried out at TCD solution concentrations of 30, 50, 100, 150, and 170 mg/mL.The stirring time, the volume ratio of antisolvent to solvent, the amount of surfactant, and the stirring intensity were separately fixed at 10 min, 4 : 1, 0.3%, and 1000 rpm, respectively.The result in Figure 2(b) shows that the particle exhibited agglomeration and the large particle size of 261.37 nm was caused by the rapid particle precipitation when the concentration of TCD solution was 30 mg/mL.However, the MPS of 130 ± 12.5 nm was obtained when the concentration of TCD solution was 170 mg/mL.In general, high supersaturation degree indicates small particle size of crystals [23,24].Therefore, the optimal concentration of TCD-ethanol solution was determined at 170 mg/mL.shows the effect of volume ratio of antisolvent to solvent on the MPS of the TCD nanoparticle.The optimization experiments were carried out at a volume ratio of antisolvent to solvent of 1, 2, 4, 6, and 8.The MPS of TCD solution was achieved at minimum, and particle size distribution was uniformed when the volume ratio of antisolvent to solvent was 2; however, high yield was not achieved at this ratio.The volume ratio of antisolvent to solvent of 4 was the optimal condition when yield is considered.
HPMC, a nonionic surfactant, was selected as the surfactant during liquid antisolvent precipitate.This surfactant can stabilize the TCD nanoparticle and prevent the occurrence of reunion phenomenon.The optimization experiments were carried out at the surfactant of TCD solution of 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%.Figure 2(d) shows the effects of the amount of surfactant on the MPS of the drug.The MPS of the drug achieved minimum when the amount of surfactant was 0.3%.The amount of surfactant was not the key factor on the MPS of drugs when it was controlled from 0.1% to 0.5%.However, the TCD nanoparticle with uniform distribution and regular shape was obtained after adding this surfactant.
Stirring intensity has a significant influence on the MPS of the TCD nanoparticle.The optimization experiments were carried out at the stirring intensity of TCD solution of 500, 1000, 1500, 2000, and 2500 rpm. Figure 2(e) clearly shows the effects of stirring intensity on the MPS of the TCD nanoparticle.When the stirring intensity was 2500 rpm, the minimum MPS of the TCD nanoparticle was obtained.The MPS of the product decreased with increasing stirring intensity.High speed stirring strengthened micromixing of liquid phases and more uniform mixing of the solution.This phenomenon promotes the rapid generation of nuclei and uniform growth of particles.Thus, better particle size distribution of the TCD nanoparticle can be obtained.The optimal stirring intensity was determined at 2500 rpm.
Based on the single-factor experiment, the optimum conditions of liquid antisolvent precipitate were as follows: stirring time was 10 min, concentration of TCD-ethanol solution was 170 mg/mL, volume ratio of antisolvent to solvent was 4, amount of HPMC was 0.3% (v/v), and stirring intensity was 2500 rpm.Under the above conditions, the TCD nanoparticle with a MPS of 130 ± 12.5 nm was obtained.All the subsequent characteristics of the optimum samples were obtained under this condition.

Morphology and MPS of TCD Nanoparticle. Figure 3(a)
shows a SEM image of raw TCD.The raw TCD particles are cuboid structure crystals with length varying from 5 m to 50 m.The SEM image of the TCD nanoparticle powder has a nearly spherical shape by liquid antisolvent precipitate under the optimum conditions from Figure 3(b).As seen from Figure 3(c), the TCD nanoparticle has a uniform distribution with a MPS of 130 ± 12.5 nm.In summary, we can obtain the TCD nanoparticle of small particle size with uniform distribution by liquid antisolvent precipitate.

Chemical Structure of TCD Nanoparticle.
The molecular structures of the raw TCD and TCD nanoparticle were examined through FT-IR spectroscopy from 500 cm −1 to 4000 cm −1 .The FT-IR spectra of the raw TCD and TCD nanoparticle without mannitol exhibited no changes in the TCD molecular structure before and after the precipitation (Figure 4(a and c)).The band assignments were as follows: 3418.50 cm −1 (associate O-H stretching vibration) and 1687.14 cm −1 (C=C stretching vibration).Only the TCD nanoparticle with mannitol showed the different spectra peak at 3265.485 cm −1 , which is because of mannitol characteristic absorption.The chemical structures of the two TCD samples were further assessed by using negative ion mode LC-MS to determine the molecular weights.As shown in Figure 5, no change occurred in molecular weight.Calculated from the mass spectrum, the molecular weights of the raw TCD and TCD nanoparticles were 148.1 and 148.2, respectively, which are the same as the molecular weight of 148.16 reported in literature.Combining the results of FT-IR and LC-MS, we determined that the chemical structure of TCD did not change before and after antisolvent.

Crystal Structure of TCD Nanoparticle.
To determine whether the drug crystal structure changed after liquid antisolvent precipitate processing, we performed XRD and DSC analyses [25].Figure 6 shows the XRD results of three TCD powders.Several distinct peaks of raw TCD at diffraction angles of 2 = 9.618 ∘ , 18.710 ∘ , 19.574 ∘ , 22.730 ∘ , 25.284 ∘ , 27.314 ∘ , 29.418 ∘ , 31.860 ∘ , and 33.776 ∘ revealed that the raw drug was present in crystalline form.However, both TCD nanoparticles and TCD nanoparticles with mannitol showed only several weak peaks at 2 = 5.560 ∘ , 18.372 ∘ , and 16.982 ∘ compared with raw TCD.These peaks disappeared or decreased, demonstrating that the crystal structure of the three samples was different.Figure 7 shows the DSC results of three TCD powders.The endothermic melting peaks of raw TCD and TCD nanoparticle were at 134.7 ∘ C and 133.6 ∘ C, respectively, which decreased 1.1 ∘ C compared to the raw TCD.The TCD nanoparticle has a small endothermic peak at 85 ∘ C, which could be the HPMC endothermic peak.And the TCD nanoparticle with mannitol has a melting peak at 160 ∘ C because of mannitol.The results indicated that the TCD nanoparticle had more amorphous state after liquid antisolvent precipitate processing.The amorphous state of the TCD nanoparticle exhibited a higher dissolution rate and bioavailability than the crystalline state of the TCD nanoparticle.The former state played a short role and increased the antioxidant capacity in vitro.

Solvent Residue Analysis.
Solvent residue problems must also be considered in pharmaceutical products.In this study, the TCD nanoparticle was prepared by the liquid antisolvent precipitate process using the International Conference on Harmonization (ICH) class III solvent ethanol with low toxicity as the solvent.The peak time of ethanol solution was at 2.945 min.A regression equation between peak () and ethanol concentration () can be fitted as  = 371.94− 91.803 ( 2 = 0.9997).According to the regression equation, the residual ethanol content in the TCD nanoparticle is 112 ppm.Given that the ICH limit for ethanol in class III solvents is 5000 ppm or 0.5%, the TCD nanoparticle met the ICH requirement and is suitable for pharmaceutical use.

Surfactant Residual Analysis. The absorbance spectrum
of TCD samples at wavelengths from 500 to 700 nm is shown in Figure 8. From the spectrum of HPMC standard solution, a regression equation between absorbance () at 634 nm and concentration () can be fitted as  = 0.004 + 0.0323 ( 2 = 0.9989).According to the regression equation, the residual HPMC content in TCD nanoparticle was 2.25%.3.8%, 73.1% ± 0.6%, and 51.5 ± 1.3%, respectively.It means that the auxiliary material was not the key factor in improving the dissolution rate of TCD.According to the Noyes-Whitney equation, dissolution rate can be proportionally increased by reducing the particle size to increase the interfacial surface area [26].In addition, the solubility of the TCD nanoparticle powder can also be increased by reducing the particle size based on the Oswald-Freundlich equation [27].Therefore, the partially amorphous and smaller drug particles exhibited higher dissolution rate and better bioavailability than crystals.
3.8.Antioxidant Activity.The results of in vitro antioxidant capability are shown in Figure 10 by DPPH free radical scavenging activity assay method.The clearance rate of TCD nanoparticle is higher than that of raw TCD nanoparticle   of the latter.This fact indicates that increase of penetration rate may be due to the decrease of the MPS of TCD.

Bioavailability Analysis.
Figure 12 shows the bioavailability results of the raw and TCD nanoparticles.The TCD concentration in rat plasma of the TCD nanoparticle group was always higher than that of the raw TCD group.These of TCD nanoparticles and raw TCD groups reached the maximum of 1.26 and 0.65 mg/mL at 5 min after taking the drugs, respectively.The bioavailability parameters are listed in Table 2.The total bioavailability within 24 hours of the TCD nanoparticles was 2.16-fold higher than that of raw TCD.The elimination rate constant () of raw TCD and TCD nanoparticles is 0.99 1/h and 1.03 1/h, respectively.The elimination half-life ( 1/2 ) of raw TCD and TCD nanoparticles is 0.70 h and 0.67 h, respectively.That can expound the onset time of TCD nanoparticles which is faster than that of raw TCD.The reasons for these clear differences of bioavailability parameters between the two drugs may be due to higher specific surface area, amorphous state, and smaller particle size.

Conclusions
In this study, liquid antisolvent precipitate process was successfully employed to prepare a TCD nanoparticle for the enhancement of dissolution rate, antioxidant activity, transdermal penetration, and bioavailability.Using ethanol as solvent and deionized water with HPMC as antisolvent was the best combination.The results indicate that the minimum MPS of the TCD nanoparticle was obtained when the concentration of TCD-ethanol solution, the volume ratio of antisolvent to solvent, the precipitation time, the amount of surfactant, and the stirring intensity were 170 mg/mL, 4 : 1, 10 min, 0.3%, and 2500 rpm, respectively.Under the optimum conditions, the MPS of the TCD nanoparticle was achieved at 130 ± 12.5 nm by single-factor analysis.The results of SEM indicate that the TCD nanoparticle obtained was small and spherical.FT-IR, XRD, DSC, and LC-MS data showed that the TCD nanoparticle had the same chemical structure as the raw drug but with lower crystallinity.The TCD nanoparticle exhibited 1.67 times faster dissolution rate and 3.33 times larger solubility than the raw drug.Furthermore, the amounts of residual solvent of the TCD nanoparticle are suitable for pharmaceutical use.The in vitro antioxidant ability of the TCD nanoparticle was 1.81 times higher than that of the raw TCD.The total oral bioavailability within 24 h of the TCD nanoparticles was 2.16-fold higher than that of raw TCD.These results suggested that TCD nanoparticle might have potential value to become a new oral or transdermal TCD formulation with high bioavailability.

Figure 1 :
Figure 1: Diagram of the experimental processes to prepare the TCD nanoparticle.

Figure 2 :
Figure 2: The effect of each parameter on the MPS of TCD nanoparticle.(a) Precipitation time.(b) Concentration of TCD solution.(c) The volume ratio of antisolvent to solvent.(d) The amount of surfactant.(e) Stirring intensity ( = 3).

Figure 2 (
Figure 2(c)  shows the effect of volume ratio of antisolvent to solvent on the MPS of the TCD nanoparticle.The optimization experiments were carried out at a volume ratio of antisolvent to solvent of 1, 2, 4, 6, and 8.The MPS of TCD solution was achieved at minimum, and particle size distribution was uniformed when the volume ratio of antisolvent to solvent was 2; however, high yield was not achieved at this ratio.The volume ratio of antisolvent to solvent of 4 was the optimal condition when yield is considered.HPMC, a nonionic surfactant, was selected as the surfactant during liquid antisolvent precipitate.This surfactant can stabilize the TCD nanoparticle and prevent the occurrence of reunion phenomenon.The optimization experiments were carried out at the surfactant of TCD solution of 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%.Figure2(d)shows the effects of the amount of surfactant on the MPS of the drug.The MPS of the drug achieved minimum when the amount of surfactant was 0.3%.The amount of surfactant was not the key factor on the MPS of drugs when it was controlled from 0.1% to 0.5%.However, the TCD nanoparticle with uniform distribution and regular shape was obtained after adding this surfactant.Stirring intensity has a significant influence on the MPS of the TCD nanoparticle.The optimization experiments were carried out at the stirring intensity of TCD solution of 500, 1000, 1500, 2000, and 2500 rpm.Figure2(e) clearly shows the effects of stirring intensity on the MPS of the TCD nanoparticle.When the stirring intensity was 2500 rpm, the minimum MPS of the TCD nanoparticle was obtained.The MPS of the product decreased with increasing stirring intensity.High speed stirring strengthened micromixing of liquid phases and more uniform mixing of the solution.This phenomenon promotes the rapid generation of nuclei and uniform growth of particles.Thus, better particle size distribution of the TCD nanoparticle can be obtained.The optimal stirring intensity was determined at 2500 rpm.Based on the single-factor experiment, the optimum conditions of liquid antisolvent precipitate were as follows: stirring time was 10 min, concentration of TCD-ethanol solution was 170 mg/mL, volume ratio of antisolvent to solvent was 4, amount of HPMC was 0.3% (v/v), and stirring intensity was 2500 rpm.Under the above conditions, the TCD nanoparticle with a MPS of 130 ± 12.5 nm was obtained.All the subsequent characteristics of the optimum samples were obtained under this condition.

Figure 3 :− 1 )Figure 4 :
Figure 3: Morphology and size distribution of TCD.(a) SEM images of raw TCD powders.(b) SEM images of TCD nanoparticle.(c) The size distribution of TCD nanoparticle suspension.

Table 1 :
Single factor and levels program.
3.7.Dissolution Rate.Figure9presents the dissolution profile of the raw TCD, TCD nanoparticles, and physical mixture of raw TCD with HPMC and mannitol.The dissolution rate of TCD nanoparticle was faster than those of raw TCD and physical mixture.After 2 h, the dissolution rates of TCD nanoparticle, physical mixture, and raw TCD were 86.0 ±

Table 2 :
The pharmacokinetic parameters of TCD by oral administration ( = 3).Area under the concentration-time profile curve until last observation.
a The elimination rate constant.b The absorption rate constant.c The elimination half-life.d Time of peak concentration.e Peak of maximum concentration.f g Area under moment curve computed to the last observation.h Mean residence time.