A Protein-Based Ferritin Bio-Nanobattery

1 Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA 2 Advanced Materials and Processing Branch, NASA Langley Research Center, Hampton, Virginia 23666, USA 3 Industrial Bioscience Department, Genencor, Shanghai, China 4 Department of Chemical Engineering, Brigham Young University, Provo, UT 84602, USA 5 Department of Physics and Astronomy, Brigham Young University, Provo, UT 84602, USA 6 National Institute of Aerospace, Hampton, VA 23666, USA


Introduction
Miniaturization of electronic devices has been a recent trend in high technology and continues to develop rapidly by enhancing efficiency, reducing power consumption, and increasing performance, speed, and functionality [1][2][3][4].The development of nanoscale devices has notably accelerated, specifically for biomedical applications [5,6].Although such devices are near reality, the development of accompanying nanoscale energy storage technology has lagged, slowing their use and benefit.The exploitation of nanodevices will depend on the development of a reliable and mobile power supply to operate them.Currently, the power systems for nanoapplications are many times larger in size than the functional devices themselves.The development of nanoenergy storage systems is needed to eliminate large power supplies and enhance the benefits accrued by miniaturizing device size.
An important feature is to embed or integrate energy storage units with their accompanying nano devices, especially when it comes to autonomous systems.In addition, biocompatibility is a desirable feature to build into emerging nano-medical devices.Various inorganic metal components are currently used in many battery applications, and this same strategy appears to be a good approach for electrical energy storage on the nanoscale.If these metallic components are easily incorporated into biocompatible biological molecules by employing bioinorganic chemistry techniques, a basic energy storage unit can be formed at the nanoscale.We describe results that have led to the development of a nano-bioenergy storage system based on ferritin bioinorganic proteins [7] that have a number of desirable features, such as a nanoscale unit size, high-power production per unit size, thin-film fabrication capabilities, potential integration with energy harvesting units, and biocompatibility.
1.1.General Background of Ferritin Properties.Ferritins are iron storage proteins widely distributed in nature that are involved in biological iron metabolism [8][9][10].Heavily loaded ferritins contain 2000-4000 Fe 3+ atoms as Fe(OH) 3 within their hollow interiors.When all iron has been removed from the protein shell it is called apoferritin; when filled with the Fe(OH) 3 mineral, it is called holoferritin.
The ferritin structure is a segmented 24-subunit, hollow protein shell with an outer diameter of 12 nm and a hollow spherical cavity 8 nm in diameter with an Mr of 430 kDa.Two types of channels (∼0.4 nm in diameter) at 3-and 4fold protein axes penetrate the 2.0 nm shell and connect the interior cavity with the external solution.In its biological role as an iron storage protein, the surrounding protein shell protects the iron mineral core from reaction with the exterior solution and the channels function as pores through this "membrane shell" and serve the unique role of ion transport.Large or negatively charged species are excluded, but small ions and molecules readily enter and leave the ferritin interior through these channels [11,12].The assembled structure of ferritins is remarkably stable to biological extremes of temperature (80 • C) and pH (2.0-10.0).

Fundamentals of Battery Operation.
Construction and operation of a typical battery consist of four basic concepts.First, two electrically isolated electrochemically active components that have different redox potentials are required, so there is a favorable thermodynamic driving force (ΔG • is negative) for electrons to transfer from the electron-rich to the electron-deficient mineral phase.If internal resistance is ignored, the difference in redox potential of these two phases determines the battery operating potential.
Second, current collecting electrodes must make electrical contact with each phase to electrically link the two isolated battery compartments and provide an external electrical pathway for electron flow.These two electrodes are the anode (−, in contact with the electron rich phase) and the cathode (+, in contact with the electron deficient phase).
Third, an ion-permeable barrier must separate and electrically isolate the two battery components.This membrane permits ion transfer between the individual battery compartments that must occur during the charging and discharging cycles as a result of the current generating chemical reactions, while electrically isolating the electrodes to prevent internal discharge.Physical separation (no membrane) of the electrodes may be sufficient if the active materials are firmly attached to the current collectors and are not mobile in solution.
The fourth requirement for efficient power storage (coulombs/gram) is to maximize the number of electrons that are transferred to and from the metal centers of the battery components.
We first present the concept of a nanobattery using ferritins with different core minerals and then describe the construction and electrochemical testing of a functional cell.The unique characteristics possessed by ferritins allowing formation of a ferritin-based battery are presented later.

Bio-Nanobattery Construction and Testing.
Figure 1 shows that two individual ferritin types, Fe(OH) 2 and Co(OH) 3 , form the two components of the bio-nanobattery and meet the four criteria outlined above for battery operation.Each ferritin type is in contact with a buffered solution, and the individual gold electrodes are separated by an ion-permeable membrane.When the circuit is closed, electrons flow through the external circuit from the anode to the cathode.During cell discharge, reaction (1) yields one electron and takes up one OH − and reaction (2) requires one electron and produces one OH − , giving the overall pH neutral reaction (3).
When Fe(OH) 2 -containing ferritin is combined with ferritin containing the Co(OH) 3 half cell, a stable opencircuit potential of ∼500 mv at pH 9.0 was measured, consistent with the redox potentials in Table 1. Figure 2 shows performance characteristics during the initial discharge cycle of this ferritin Fe(OH) 2 /Co(OH) 3 bio-nanobattery as summarized by reactions (1)- (3).When discharged at closed circuit at 100 namps (Figure 2), all electrons were transferred from Fe(OH) 2 to Co(OH) 3 according to reaction (3): Fe(OH) 2 + Co(OH) 3 = Fe(OH) 3 + Co(OH) 2 . ( We have also constructed and tested a battery system using Mn(OH) 3 in place of Co(OH) 3 under the same conditions, which performs similar to that shown in Figure 2. Other results with both Co(OH) 3 and Mn(OH) 3 are considered next in a different application.
The cell just described is a "bulk" electrochemical cell because the ferritin concentrations exceed the amount of ferritin that can bind to the gold electrodes and demonstrates  the feasibility of using ferritins as battery components.However, diffusion of ferritins to the current collecting electrodes in such a cell becomes a limiting factor in current flow.In an attempt to miniaturize the cell size to minimize diffusion and to eliminate excess ferritin beyond that which binds to the electrode, we investigated ferritin bound only to the electrode surface.With this approach, a nanobattery consisting of only surface-bound ferritin molecules can be constructed whose size and charge capacity are determined by the electrode surface coverage and the smallness of the gold line onto which the ferritins bind.During attempts to create such a small cell, different electrical properties were encountered than those observed for the bulk cell.Contrary to previous reports [13][14][15], purified ferritin in the presence of a clean gold electrode did not demonstrate the ability to rapidly oxidize or reduce the mineral core during cyclic voltammetry, as shown as the left curve in Figure 3. Electrochemical reactivity of redox proteins can be augmented by addition of chemical promoters, which catalytically facilitate electrochemical reactions [16].Addition of thioglycolic acid (TGA) at <0.01 mM to a ferritin solution, while applying an adsorbing potential of +500 mV (SCE), caused ferritin to adsorb onto the gold electrode and exhibit electro activity shown by the right curve in Figure 3.
Figure 4 shows AFM images of ferritin adsorbed at +500 mV both without (a) and with (b) the TGA promoter.Figure 5 shows the discharge characteristics of a Mn(OH) 3 /Fe(OH) 2 cell composed only of surface adsorbed ferritins in the presence of TGA promoter, and the charge transferred during discharge was 90 µC.This corresponds to 500 electrons transferred per ferritin, which was equivalent to the concentrations of Fe 2+ -and Mn 3+ -ferritins present in the bound ferritins.The smaller loading was used to demonstrate that all electrons were removed even from smaller cores.
Several different ferritin-binding configurations using two different ferritin types (Co and Mn) bound to different electrodes were also investigated.Surface adsorbed ferritins containing Fe(OH) 2 and Co(OH) 3 or Mn(OH) 3 on gold electrodes typically produced >420 mV open circuit.
The facile electrochemical reactions observed in the absence of promoter shown in Figure 2 for the bulk battery stands in contrast to the promoter-requiring behavior seen in Figure 5.This behavior may have an explanation resulting from the methods of preparation of apoferritins and to the relative amounts of each used in the respective experiments.Apoferritins are prepared by removal of the iron mineral cores with TGA [8][9][10] or sodium dithionite, both of which function as promoters.Although rigorous processing is typically carried out to remove these constituents, it is possible that very small amounts remain free or ferritin bound in the ferritin solution.The use of bulk quantities of ferritin in Figure 2 compared to surface quantities used in Figure 5 would provide for more promoters to be available in the bulk cell, causing it to function without added promoter (but to function by "adventitious promoters") compared to experiments with only surface bound ferritins.In any case, the results show that ferritins can function as electrochemical cell components (in bulk or surface-bound) in an electrochemical energy storage role.
To assure permanent attachment and to increase the surface coverage for greater charge capacity, ferritins were chemically attached to the gold surface.When subjected to negative voltage cycling in the presence of Fe 2+ chelators, the Fe 3+ in the chemically attached holoferritin was reduced and the resulting Fe 2+ was immediately chelated forming apoferritin [14,15].When this process was reversed in anaerobic buffer containing Fe 2+ and cycled in the oxidizing direction, holoferritin was once again formed [14].Both redox cycling experiments were performed in the absence of added promoters or mediators, demonstrating direct electron transfer from electrode to mineral core through the chemically bound protein shell.Both physical adsorption and chemical attachment of ferritin to gold electrodes led to electroactive ferritin layers, but chemical attachment was more stable.Chemical attachment produced slightly lower open-circuit voltages.
The results show that the minimum requirements for a ferritin-based nanobattery are the attachment of two different ferritin types to two different gold electrodes both in close proximity and in contact with a minimal volume of buffer necessary to support reactions (1)-( 3) during cell function.
The size of the biobattery can be scaled to very small dimensions and is limited only by the size of an inscribed gold line onto which the ferritins are adsorbed or chemically tethered.If chemical attachment is used, no membrane is required as long as the electrodes are physically separated because the lack of mobility of the chemically bound ferritin precludes interaction.For physically adsorbed ferritin, a membrane is desired because internal cell discharge can occur if anodic and cathodic ferritins in close proximity exchange between solution and electrode [17].
The minimum battery unit consisting of one anodic and one cathodic ferritin molecule as shown in Figure 1  be calculated (excluding buffer) using the fundamental components in Figure 1.
An ongoing approach for improving the coulombic capacity of a ferritin nanobattery is presented in Figure 6, which shows a native ferritin that is negatively charged due to its ionized surface carboxylate groups at pH 7-9 first adsorbed or chemically tethered to an electrode surface.
Next, ferritin is "cationized" by having its surface carboxyl groups modified by attaching -NH 3 + groups.This cationized ferritin binds to the negatively charged "basement" ferritin layer attached to the electrode, forming a second, positively charged layer.By alternating ferritins of different charges, a layered electrode is formed.At this stage of development, five alternate layers have been formed as shown by the AFM and SEM images in Figure 6, demonstrating the feasibility of a multilayer nanobattery.

Materials and Methods
Both holo and apo horse spleen ferritin (HoSF) were obtained from Sigma and were further purified and characterized as previously reported [13,18,19].Ferritin containing Fe(OH) 2 mineral cores was prepared [20] either by reduction using carbon monoxide dehydrogenase (CODH/CO, reaction (4)) or MVH 2 as summarized by the reaction sequences (4)-( 5) and ( 6)- (7), respectively.Co(OH) 3 and Mn(OH) 3 were prepared as previously described [21]: MVr + Fe(OH) 3 = MV + Fe(OH) 2 + OH − , ( MV + MVH 2 = 2MVr + 2H + , ( MVr + Fe(OH) 3 = MV + Fe(OH) 2 + OH − .( Because ferritin with a Fe(OH) 2 mineral core is highly susceptible to O 2 -oxidation, all preparation and handling procedures were conducted in vacuum atmospheres glove boxes with (<1.0 ppm O 2 ).Bulk cell measurements were conducted in a twochambered Lucite cell separated by a 10 KDa cellulose membrane.One chamber contained ∼1.0 mL of an anaerobic Fe(OH) 2 -ferritin and the other a solution of Co(OH) 3or Mn(OH) 3 -ferritin in 0.05 M Mops buffer pH 7.0-9.0containing 0.10 M NaCl.The ferritins were at 5-20 mg/mL and contained 500-2000 metal atoms per ferritin core.Each chamber also contained a gold electrode, which was connected through an external circuit.For electrodes containing only surface-bound ferritins, the gold electrodes were first cleaned in Piranha solution, rinsed, and placed in the desired anaerobic ferritin solution containing 0.01-0.10mM thioglycolic acid (TGA).The electrodes were then equilibrated at +500 mV to adsorb the ferritins.Each electrode was rinsed anaerobically to remove unbound ferritins and free TGA.Electrical measurements were made using a EG&G PAR 263A potentiostat/galvanostat controlled by Power Suites software.

Discussion
Having established that "bulk," "surface-adsorbed," and "chemically tethered" ferritin-containing electrodes are readily constructed and that their performance is consistent with known half-cell values in Table 1, we now discuss the unique ferritin characteristics that have led to the concept and eventual fabrication of a functional ferritin bio-nanobattery.These concepts form the basis for future development and improvement.

Chemical Reconstitution of Ferritins for Cathode and
Anode Materials 4.1.1.Anode Materials.Viable candidates for bio-nanobattery anode materials are oxyhydroxides of light transition metals with low reduction potentials that are compatible with known ferritin properties.Iron was first considered, but the oxidation state of iron in isolated ferritin is Fe(OH) 3 and does not yield electrons at low potential.However, the native Fe(OH) 3 mineral core undergoes [20] a pH-dependent reduction (−120 mv/pH, pH 7.0-9.0,Table 1) to form Fe(OH) 2 .The Fe(OH) 2 mineral core is stable [17,20,22] within the ferritin interior at pH > 7.0.The absence of Fe 2+ chelators is critical to maintaining the Fe(OH) 2 mineral core, and a number of methods to produce stable Fe(OH) 2 have been explored [20,22,23].The MV-mediated chemical procedures outlined in the Materials and Methods Section are convenient methods for core reduction.The Fe(OH) 2 mineral core is readily oxidized to Fe(OH) 3 with O 2 and other oxidants [10,18], demonstrating redox reversibility.
For chemically attached ferritins, the mineral cores undergo direct electron transfer via electrochemical voltage cycling without need for chemical reduction (Figure 3).Ferritin with an Fe(OH) 2 mineral core was used exclusively in the experiments described here because of its availability and convenience of formation, but other more strongly reducing mineral cores are desirable and are being evaluated.The phosphate-containing mineral core from Azotobacter vinelandii is readily reduced to Fe(II) and retained within the protein cavity [19,23,24].This iron (II) core is more strongly reducing at pH 7.0, but because it is not as readily available, its use as a bio-nanobattery anode has been limited.An important difference between animal and the phosphatecontaining bacterial ferritins is that animal ferritin has a pH-dependent reduction potential whereas that for bacterial ferritin is pH independent [19,20].Therefore, the voltage output from an electrochemical cell made from animal ferritin is tunable with pH, whereas that for bacterial ferritin is not.
Reconstitution using variations of this site-specific biomineralization process produces ferritins with an impressive variety of interesting and possibly technologically useful, but nonnatural, nanomineral cores [25][26][27][28].Of interest in creating a bio-nanobattery are ferritins containing the oxidizing Co(OH) 3 and Mn(OH) 3 mineral cores with 500-2000 metal atoms per ferritin [21,29].Chemical and electrochemical characterization (Table 1) established that these mineral cores are reduced at potentials near +50 mv with retention of the Co(OH) 2 and Mn(OH) 2 minerals [22].The stability of M(OH) 2 (M = Fe, Co, Mn, Ksp = ∼10 −16 ) is quite remarkable and is likely due to both kinetic and thermodynamic effects.M 2+ and M 3+ bind strongly within the ferritin channels, and, once the M(OH) 2 core is formed, transfer of M(OH) 2 to the external solution is blocked [10] by channelbound M 2+ .
Co(III) and Mn(III) ferritins when combined with Fe(OH) 2 successfully functions as cathodic components producing a bulk cell potential of ∼500 mV.Higher cell potentials are desired and can be achieved in two ways.The anode material could be more strongly reducing, or the cathodic component could be more strongly oxidizing or a combination of both.The use of Ni(OH) 3 as cathodic material could produce a predicted cell potential near 1.0 volt.Our best results give a mixed mineral core with ∼50% Ni(OH) 3 and Ni(OH) 2 , and with this mixture a higher cell voltage is produced as predicted but a well-defined system remains elusive.

Current Collecting Electrodes.
Intimate contact of anodic and cathodic cell components with electrodes is essential for efficient electron transfer and also for lowering internal cell resistance.The contact of ferritin with the electrode and requisite electron transfer from the core material through the protein shell initially seemed to be serious limitations in battery operation.However, two unique properties of the ferritin protein shell eliminate these restrictions and allow for specific interaction with electrodes.
Previous studies have shown [17] that rapid electron transfer occurs through the HoSF shell.To explain this apparent anomalous behavior, an endogenous electron transfer pathway was postulated [30], consisting of a protein redox center involving tryptophan 91.This electron transfer characteristic of the ferritin protein shell was demonstrated when Fe(II)-ferritin was reacted with Co(III)-ferritin, both free in solution, and within seconds Fe(III)-ferritin and Co(II)-ferritin were formed by electron transfer through the two intervening protein shells [17].Even faster electron transfer was observed with bacterial ferritins, presumably through the 12 protoporphyrin IX groups.Electron transfer was further enhanced [17] by addition of a small piece of metallic gold.This enhancement occurred by Fe(OH) 2 and Co(OH) 3 ferritins binding to and exchanging electrons through the same gold conductor instead of randomly contacting each other in solution, as suggested by Figure 7.
The protein shells thus protect the mineral cores from contact with bulk solution while simultaneously providing electron transfer capability through the protein shell.

Physiochemical Adsorption.
Binding of ferritins to metallic and nonmetallic surfaces has been extensively studied [31][32][33][34].The adsorption process is essential in creating a high-density surface layer, which provides for high current density on the current collecting electrodes.Each ferritin subunit possess a cysteine at position 90, and when assembled into the 24-mer, one cysteine from each of three subunits is positioned at the corners of a triangle at the outside entrance of each of the eight 3-fold channels.
As there are eight threefold channels per symmetrical ferritin molecule, all ferritin molecules are strongly bound and attached identically to the electrode surface.Binding at the 3-fold channel also properly orients the ferritinelectrode interaction to maximize electron transfer through the proposed electron transfer pathway through the protein shell [17].However, in spite of this natural binding affinity, it seems that promoters are also required to facilitate electron transfer.

Chemical Attachment.
Chemical immobilization of ferritin on gold electrodes is achieved through two different procedures [35,36].The first directly attaches a terminal thiol group to the ferritin exterior via reaction with thiol-containing organic molecules followed by attaching the thiolate group on the gold electrode.The second method requires modification of the Au electrode with thiol-derivatized molecules such as dithiobis-N-succinimidyl propionate (DTSP) and then reaction of the amino surface groups of ferritin with the thiol-derivatized gold surface.Using these methods, highly concentrated and electroactive ferritin layers are obtained [14].

Electrical Conductivity of the Core Materials.
In addition to the observed electron transfer through the protein shell, it is also essential that the mineral core be sufficiently conducting that all electrons flow from the mineral through the protein shell and then to the electrode.Core conductivity of the ferritin mineral was established [37,38] when 57 Fe 2+ was added anaerobically to Fe(III)-containing native mineral cores, and 57 Fe 3+ was observed by Mossbauer spectroscopy.This result showed that electrons were transferred from the entering 57 Fe 2+ to and delocalized into the bulk Fe(OH) 3 mineral core, implying mineral conductivity.A definitive demonstration of the conductivity of both the mineral core and the protein shell was established by placing a conductive atomic force microscope tip on single molecules of holo-and apoferritin and showing that the conductivity of holoferritin was 3-4 times greater than the apo counterpart [39].

The Ferritin Shell Is an Ion Permeable Membrane:
The Ferritin Channels.X-ray crystallography [40,41] of apo ferritin shows the presence of channels at three-and fourfold axes (0.3-0.4 nm in diameter) where three and four subunits intersect.The hydrophylic 3-fold channels are used for ion transfer into and out of the ferritin but the role of the hydrophobic 4-fold channels remain unclear.However, that ions and molecules enter and leave through these channels is well documented [12].
Trapping large organic dye molecules and ferricyanide in the ferritin interior established [11] that their large size precluded their release.Studies [12] with nitroxide molecules confirmed this view by demonstrating that they only slowly exchanged (hours) with the ferritin interior.Recently, it was shown that Fe 2+ and Fe 3+ rapidly enter into the ferritin interior through the channels [42].Other studies suggest [43] that the channel openings are fluxional and may actually change dimensions under certain conditions, which facilitates ion transfer.Such studies establish that the channels make effective transport pathways for ions and small molecules between the exterior solution and the protein cavity.The transfer of OH − into and out of the ferritin interior during battery operation via these channels (reactions (1)-( 3)) is an essential property of ferritin that made creation of a bio-nanobattery possible.

Further Nanobattery Development.
Construction of functional nanobatteries has not been extensively reported.Stanish et al. reported [44] a biological battery composed of polymerized vesicles, having benefits of biocompatibility with the potential to assemble on nanopatterned arrays.The maximum operating voltage of this vesicle biobattery was 28.9 mV with a charge capacity of 127 nC.
In contrast, the ferritin-based bio-nanobattery is distinct from the vesicle battery.Densely loaded Fe-and Co-or Mnloaded mineral cores were used as half-cells, and electron flow occurred from the mineral phases through the protein shells to current collecting electrodes.The voltage output was 16 times larger, and the coulomb capacity was ∼1400 times larger than the vesicle battery making it a more useful energy storage device.
The bio-nanobattery system depicted in Figure 1 does not require a membrane if only sufficient ferritin is present to strongly bind to the metal surfaces because the ferritin channels act as ion-permeable membranes, and both electrodes can be in close proximity.Using self-assembling characteristics of ferritins, an array of ferritin units can be assembled into well-organized multilayered ferritins.Figure 6 shows how this approach is applied to increase the capacity of a biobattery by binding multiple layers of ferritins to the electrode surface.The unique ability of electrons to transfer through the intervening protein shells [17] makes possible the stacking of multiple layers of ferritins and still provides for the outermost layer to readily transfer electrons to the metallic electrode.
In this discussion, we have presented two types of battery arrangements and each has advantages and disadvantages.A battery with two half-cells separated by an ion-conducting membrane has a coulombic capacity that depends on the volume and the concentration of the ferritins in the solution.The disadvantage of this arrangement is that remote ferritins must diffuse to the electrode to exchange electrons.While electron exchange readily occurs, diffusion can be a limiting factor in battery function.On the other hand, a battery lacking a membrane has the advantage of being simpler and more responsive because the ferritins are bound directly to the electrode surface, which eliminates diffusion and gives responsive battery performance.Another advantage is that this type of bio-nanobattery can be scaled to the size of a small inscribed gold line.The disadvantage of this arrangement is that such a small size limits the number of bound ferritins, which limits the coulombic capacity of the battery.The stacking of ferritins as proposed in Figure 6 somewhat mitigates this disadvantage.It is likely that both types of bio-nanobatteries will find useful but quite diverse applications.

Figure 1 :
Figure 1: Schematic view of a bio-nanobattery formed from ferritin containing a Fe(OH) 2 mineral core connected to a ferritin containing a Co(OH) 3 mineral core.The cutout view shows the mineral cores within each ferritin type.The ferritin battery components are immersed in buffer, which is typically 0.05 M pH 7.0-9.0containing 0.10 M NaCl.

Figure 2 :
Figure 2: Voltage versus time discharge curve.A small, unstirred bulk electrochemical cell consisting of ferritin containing a Fe(OH) 2 mineral core connected to a ferritin containing Co(OH) 3 mineral core in 0.05 M MOPS pH 8.0 was discharged at a drain rate of 100 nA.Each ferritin contained ∼2000 metal ions per mineral core.

Figure 5 :
Figure5: Discharge curve of a cell containing 500 Fe(OH) 2 and 500 Mn(OH) 3 attached to gold electrodes by deposition at +500 mV in the presence of TGA.The solid line is an exponential fit to the data and was used to calculate that 90 µcoulombs transferred through the cell.

Figure 6 :
Figure 6: A multilayered ferritin bio-nanobattery for high coulomb capacity, high efficiency, compact size, and flexible applications.The anodic and cathodic battery components are formed by alternating layers of oppositely charged ferritins.The blue is the anodic layer, and the red is the cathodic layer.The yellow membrane between the two layers is not needed if excess ferritins are not present.The image on the upper right is a SEM image of five layers of ferritin.The image on the lower right is an AFM image of five ferritin layers.

Fe 3 Figure 7 :
Figure 7: Electron transfer through the ferritin protein shell.The blue dot in the cut-away view of the protein shells containing Fe(OH) 2 (pink) and Co(OH) 3 (purple) mineral cores represents the electron transfer centers within the ferritin shell.The model on the left indicates that the two electron transfer centers are not in contact and do not provide a pathway for electron exchange.The model on the right shows the two redox centers aligned and capable of exchanging electrons from the two mineral cores.The models only show one of several possible electron transfer centers present in each ferritin protein shell.

Table 1 :
Electrochemical properties of ferritins with different core minerals.