Journal of Nanotechnology
Volume 2020 (2020), Article ID 2932434, 10 pages
https://doi.org/10.1155/2020/2932434

Research Article

Synthesis of Copper Oxide Nanoparticles Using Plant Leaf Extract of Catha edulis and Its Antibacterial Activity

Worku Wubet Andualem,¹ Fedlu Kedir Sabir,² Endale Tsegaye Mohammed,³ Hadgu Hailekiros Belay,² and Bedasa Abdisa Gonfa²

¹Arba Minch University, College of Natural Sciences, Department of Chemistry, P. O. Box: 21, Arba Minch, Ethiopia
²Adama Science and Technology University, School of Applied Natural Science, Departments of Applied Chemistry, P. O. Box:1888, Adama, Ethiopia
³Hawassa University, College of Natural and Computational Science, Department of Chemistry, P. O. Box: 05, Hawassa, Ethiopia

Correspondence should be addressed to Bedasa Abdisa Gonfa; bedassa.abdissa@astu.edu.et

Received 16 April 2020; Revised 20 August 2020; Accepted 6 November 2020; Published 24 November 2020

Academic Editor: María J. Lázaro

Copyright © 2020 Worku Wubet Andualem et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Development of green technology is generating interest of researchers towards ecofriendly and low-cost methods for biosynthesis of nanoparticles (NPs). In this study, copper oxide (CuO) NPs were synthesized using a copper nitrate trihydrate precursor and Catha edulis leaves extract as a reducing and capping agent during the synthesis. The biosynthesized CuO NPs were characterized using an X-ray diffractometer (XRD), scanning electron microscopy-energy-dispersive X-ray spectroscopy (SEM-EDS), transmission electron microscope (TEM), Ultraviolet visible spectroscopy (UV-Vis), and Fourier transform infrared (FTIR) spectroscopy. XRD characterization confirmed that the biosynthesized CuO NPs possessed a good crystalline nature which perfectly matched the monoclinic structure of bulk CuO. Furthermore, the results obtained from SEM and TEM showed that the biosynthesized CuO NPs were spherical in shape. EDS characterization of the biosynthesized NPs also indicated that the reaction product was composed of highly pure CuO NPs. Moreover, the antimicrobial activities of different concentrations of CuO NPs synthesized using Catha edulis extract were also tested. Accordingly, the result showed that the highest zone of inhibitions measured were for CuO NPs synthesized using 1 : 2 ratios at 40 mg/ml solution concentration and observed to be 22 ± 0.01 mm, 24 ± 0.02 mm, 32 ± 0.02 mm, and 29 ± 0.03 mm for S. aureus, S. pyogenes, E. coli, and K. pneumonia, respectively.

1. Introduction

The emergence of infectious diseases in general causes a serious threat to public health worldwide especially with the emergence of antibiotic-resistant strains of bacteria. Gram-positive and Gram-negative bacterial strains are considered as presenting a major public health problem, and as a result, antibiotics have been used to control infections resulting from community and hospital settings [1]. With the emergence of microorganisms resistant to multiple antimicrobial agents, there is a growing interest in developing new bactericides based on inorganic materials to substitute the traditional organic agents as organic agents have limited applications due to their low heat resistance, high decomposability, and short life [2, 3]. Nowadays, metal oxide nanoparticles (NPs) such as copper oxide (CuO), iron oxide (Fe₃O₄), and zinc oxide (ZnO) have drawn attention due to their unique optical, physical, and biological properties [4–6]. Among these metal oxide NPs, CuO NPs have become a focus of interest because they play a significant role in environmental remediation and biopharmaceutical industries [7]. CuO NPs can be synthesized using various physical and chemical methods such as electrochemical reduction [8, 9], sonochemical methods [10–12], chemical precipitation [8], solution plasma, thermal decomposition [11], microwave irradiation [13], and the sol-gel method [14–16]. Even though these synthesis methods of NPs are easy and provide high yields, the use of toxic chemicals as reducing agents remains a major concern. In certain methods, the use of nonpolar solvents for the synthesis of CuO NPs amplifies the toxicity of the NPs. Therefore, utilizing biological sources for the synthesis of NPs could provide a favorable, nontoxic, and ecofriendly approach [17].

Biological methods of synthesis of NPs using microorganisms such as algae, fungi, bacteria, and plant leaves extracts have been suggested as possible ecofriendly alternatives to chemical methods as these methods are of low cost, energy efficient, and nontoxic [18]. Use of plant extracts for synthesis of NPs could be advantageous over other environmentally benign biological processes as this eliminates the elaborate process of maintaining cell culture [19]. Furthermore, the advantage of using plants for the synthesis of NPs is that they are easily available, safe to handle, and possess a broad variability of metabolites that may aid in reduction [20].

Based on the previous literature reports, CuO NPs were synthesized from various plants extracts such as Acalypha indica [21], Phyllanthus amarus [22], Terminalia arjuna [23], Calotropis gigantean [24], Malva sylvestris [25], Cassia alata [26], Gloriosa superba [2], and Carica papaya [27], and their antimicrobial activities were also reported.

In the present study, Catha edulis leaves extracts were considered for the synthesis of nanoparticles as this is the first ever detailed report on the plant being utilized for green synthesis of NPs. Catha edulis, commonly known as khat, qat, chat, or miraa, is a small-to-medium-sized evergreen tree that belongs to the Celastraceae family [28]. Some oral traditions claim that khat originated from Yemen; however, the literature report indicates that khat originated from Ethiopia, specifically in Hararghe, with a gradual expansion to different parts of Ethiopia, Yemen, and other parts of the world [29]. The leaves of this small tree are chewed by millions worldwide as a mild, amphetamine-like psychostimulant drug [30]. The chemical constituents of Khat include alkaloids, terpenoids, flavonoids, sterols, glycosides, tannins, amino acids, vitamins, and minerals [30, 31]. Studies have indicated that biomolecules such as proteins, phenols, and flavonoids not only play a role in reducing the ions to nanosize but also play an important role in the capping of NPs [32]. Therefore, this work aimed to explore the application of Catha edulis leaves extract as a capping and reducing agent for the synthesis of CuO NPs in addition to its consumption as amphetamine-like psychostimulant drug worldwide and evaluate the antibacterial activities of the synthesized CuO NPs against selected pathogenic organisms using the agar disc diffusion method. Furthermore, this work opens up a door for further explorations on many of Ethiopian-origin plants for nanomaterial synthesis which could be used for various applications.

2. Materials and Methods

2.1. Biosynthesis of CuO Nanoparticles

Fresh leaves of Catha edulis were collected from rural farm areas and washed with distilled water to remove the dust particles. The cleaned leaves were dried for 7 days at room temperature under shade and then ground into a fine powder using a mortar. 10 g of Catha edulis powder was taken for extraction purpose and boiled with 150 mL of distilled water for 30 min at 80, and the color of the aqueous solution was changed from watery to light brown. The extract was cooled at room temperature and filtered by using Whatman No 1 filter paper. Then, three different samples with different precursor salt to Catha edulis extract ratios (w/v) were prepared by taking different weights of the salt precursor and volume of Catha edulis leaf extract using a previously developed procedure [2]. Accordingly, three 100 mL Catha edulis leaf extracts were added into three different volumetric flasks and heated to a temperature of 80 while stirring using a magnetic stirrer. After the solution temperature of the extract reached 80, 10, 30, and 50 g of copper (II) nitrate trihydrate (UNI-CHEM Chemical Reagents) precursor salt were added to the first, second, and third leaf extracts, respectively. The three samples were, then, labeled as 1 : 10 (10 g: 100 ml), 3 : 10 (30 g: 100 ml), and 1 : 2 (50 g: 100 ml) ratios, respectively. Then, for each sample, the mixture was stirred continuously using a magnetic stirrer at 80 until the color was changed from deep green to deep brown and precipitate was observed. The precipitate of each sample was, then, centrifuged at 1000 rpm for 30 min and washed with deionized water to remove impurities and centrifuged again for 20 min. Finally, each precipitate was dried in an oven at 150 for  hrs and was ground to a fine powder using an agate mortar. The black powder obtained from the abovementioned method was calcinated at 400 for 2 hrs.

2.2. Characterization of Biosynthesized CuO Nanoparticles

The morphology and composition of the synthesized CuO NPs were characterized by field emission scanning electron microscopy (FE-SEM, JEOL-JSM 6500F, made in Japan), FESEM coupled energy-dispersive X-ray spectroscopy (EDX), and a transmission electron microscope (HR-TEM, Tecnai F20 G2, Philips, the Netherlands). Crystalline structure and the average crystalline size of the synthesized CuO NPs were characterized using an X-ray diffractometer (XRD-7000, Shimadzu Co., Japan) equipped with a Cu target for generating a Cu Kα radiation with (λ 1.54056 Å). XRD spectra were recorded from 10 to 80 with 2θ angles using CuKα radiation operated at 40 kV and 30 mA. The optical absorption spectra of the synthesized CuO NPs were recorded using a UV/Vis (JASCO V-670UV-Vis spectrophotometer equipped with a diffuse reflectance attachment for powder samples) spectrophotometer. For functional groups analysis, the Fourier transform infrared (FTIR) spectrum was recorded using the Perkin Elmer 65 in the range of 4000–400 cm⁻¹.

2.3. Antibacterial Activity Analysis of Biosynthesized CuO NPs

In vitro antibacterial activity for different concentrations of the biosynthesized CuO NPs and crude extract of Catha edulis against Gram-positive (Staphylococcus aureus (ATCC25923) and Streptococcus pyogen (ATCC700603)) and Gram-negative (Klebseilla pneumonia (ATCC 19615) and Escherichia coli (ATCC 25922)) bacterial strains was tested by the disc diffusion method. These bacterial strains were cultured and obtained from the Oromia Region Health Bureau regional laboratory, Adama, Ethiopia. All the bacterial strains were maintained at 4 on nutrient agar slants. The bioassay used was the standard disk diffusion assay on Mueller Hilton Agar Media [33, 34]. Test disks were prepared by dipping and saturating sterilized filter paper discs in the test suspensions. All the Petri dishes were sealed with the sterile laboratory parafilm to avoid eventual evaporation of the test samples. A standard commercial Gentamicin was used as positive control, and discs impregnated with DMSO was used as negative control. The plates were incubated at 37 overnight in an incubator and were shaken gently to allow even mixing of bacteria cells and agar. Then, 20 and 40 mg from each of the three ratios of CuO NPs (1 : 10, 3 : 10, and 1 : 2) were taken and dissolved in 1 mL of DMSO to obtain 20 and 40 mg/mL solutions. From each different ratio, 100 μL was taken and saturated with discs (5–6 mm diameter) and incubated at 37 for about 24 hrs. In antibacterial testing against each strain, triplicate measurements were performed and the average value was used in reporting the results.

3. Results and Discussion

3.1. Thermal Gravimetric Analysis (TGA)

The as-synthesized CuO NPs using copper nitrate trihydrate precursor salt and Catha edulis leaves extract were characterized by using TGA/DTA. The weight losses of the investigated materials were analyzed. Figure 1 shows the thermo gravimetric analysis and differential thermal analysis of the as-synthesized CuO NPs.

Figure 1: Thermal analysis of as-synthesized CuO NPs using Copper (II) nitrate trihydrate and Catha edulis synthesized using 1 : 10 ratio (w/v).

The TGA curve shows mass loss of the sample, whereas the DTA curve indicates the energy gain or loss during the process. As it can be observed from Figure 1, the TGA curve of CuO NPs showed three-step decomposition, and weight losses were observed in the temperature ranges of 25–150, 400–600, and 601–1000. The weight loss of the material observed in the temperature range of 25–150 was about 11.594%, and this was mainly due to vaporization of water content from the sample. The sample also showed a weight loss of about 1.35% in the temperature range of 400–600 that could be due to combustion of biomolecules from the Catha edulis extract that remained in the biosynthesized NPs. The weight loss observed above 600 was about 2.626% that may be due to escaping of oxygen from the sample [19]. In general, from TGA analysis, the total weight loss calculated was 15.570%, and the TGA results suggested that this compound is thermally stable and it has negligible weight loss when compared to the bulk sample of CuO NPs.

3.2. X-Ray Diffraction (XRD) Analysis

Figure 2 shows the XRD pattern of CuO NPs synthesized from copper (II) nitrate trihydrate and Catha edulis leaf extract using three different ratios (w/v). The formation of biosynthesized CuO NPs was confirmed by X-ray diffraction measurements. The diffraction peaks observed at 2θ 31.4876°, 35.4894°, 38.6974°, 48.7540°, 53.4330°, 58.2323°, 61.5322°, 66.2470°, 67.6419°, 72.3621°, and 75.2398° in Figure 2(a), 32.4911°, 35.5036°, 36.4007°, 38.4007°, 48.8047°, 53.4321°, 58.2223°, 61.5010°, 65.7825°, 67.9854°, 72.3607°, and 75.7198° in Figure 2(b), and 31.6751°, 35.5086°, 38.7159°, 48.8047°, 53.4321°, 58.2223°, 61.5010°, 66.2656°, 67.4820°, 72.3607°, and 75.2598° in Figure 3(c) were corresponding to (100), (002), (200), (202), (020), (202), (113), (022), (020), (311), and (004) crystal planes, respectively, which is in agreement with the previously reported work [3, 17, 21, 24].

Figure 2: XRD patterns of CuO NPs synthesized using copper (II) nitrate trihydrate and Catha edulis leaf extract of different ratios (w/v): (a) 1 : 10, (b) 3 : 10, and (c) 1 : 2.

(a)

(b)

(c)

Figure 3: SEM images of CuO NPs synthesized using copper (II) nitrate trihydrate and Catha edulis leaf extract in different ratios (w/v) (a) 1 : 10, (b) 3 : 10, and (c) 1 : 2.

The observed diffraction reflections are comparable with JCPDS card № 048–1548, 048–1548, and 048–1548 for each ratio and is attributed to bulk CuO materials [25]. All diffraction peaks can be indexed as the typical monoclinic structure CuO NPs. The absence of extra diffraction peaks in the XRD patterns in Figures 2(a) and 2(c) shows the purity of the biosynthesized CuO NPs. However, the appearance of additional diffraction peaks observed in Figure 2(b) at about 2θ 36.4° and 44.9° (with reference to ICSD file no. 98-015-4604) indicates the existence of the Cu₂O at the surface [35]. Moreover, the well-defined and sharp CuO reflections observed from XRD patterns confirms the good crystalline nature of the biosynthesized CuO NPs. Similar results were also reported in previous similar works [19, 27, 36]. The crystal size of the biosynthesized CuO NPs was calculated using Debye Scherrer’s formula. As a result, the crystal sizes of CuO NPs corresponding to the highest intense peaks for 1 : 10, 3 : 10, and 1 : 2 were calculated and found to be 28.10 nm, 25.30 nm, and 18.20 nm, respectively.

3.3. Scanning Electron Microscopy-Energy-Dispersive Spectroscopy (SEM-EDS) Analysis

The surface morphologies of biosynthesized CuO NPs were studied by SEM, and the results are presented in Figure 3. Figure 3(a) is a SEM image of CuO NPs synthesized using 1 : 10 ratio (w/v) which showed uniform and defined spherical-shaped NPs with low aggregation. The low aggregation could be due to the high fraction of plant extract used which acts as a capping/stabilizing agent. Similar to the 1 : 10 ratio, Figure 3(b) shows the SEM image of CuO NPs synthesized using 3 : 10 in which the NPs possessed spherical shapes with some aggregation/agglomeration as the concentration of the plant extract that acts as a stabilizing agent used was decreased relative to the amount of salt precursor used. Furthermore, the SEM image of the biosynthesized CuO NPs using 1 : 2 ratios (w/v) is also shown in Figure 3(c). As it can be observed, this SEM image showed a similar feature with CuO NPs synthesized using 1 : 10 and 3 : 10 ratios but with increased aggregation/agglomeration of the NPs. The high aggregation/agglomeration of the NPs may be caused due to polarity and electrostatic attraction of CuO NPs as the result of increased concentration of the salt precursor [37]. In general, it is noticed from the SEM images analysis that the biosynthesized CuO NPs showed small and uniform-sized spherical NPs which is in agreement with the previously reported work [19, 35, 38].

To gain further insight into the features of the biosynthesized CuO NPs, the chemical composition of the NPs was analyzed using EDS (Figure 4). The energy-dispersive spectra of the samples obtained from the SEM-EDS revealed that the sample prepared by using Catha edulis plant extract has pure CuO phases.

(a)

(b)

Figure 4: SEM images of the selected area for EDX spectra (a) and quantitative analysis (b) of CuO NPs synthesized using copper (II) nitrate trihydrate and Catha edulis leaf extract in1:10 ratios (w/v).

The EDS studies of Figure 4 present three peaks between 1 kV and 10 kV that are directly related to Cu in the tested material [39]. The results indicate that the reaction product is composed of high purity CuO NPs which agrees with the result obtained from XRD (Figure 2). The weight composition obtained from EDS analysis of the normalized spectrum was Cu (75.62%) and oxygen (24.38%). EDS also revealed the formation of nonstoichiometric CuO NPs with oxygen vacancy which could lead to better antibacterial activity.

3.4. Transmission Electron Microscope (TEM) Analysis

Figure 5 shows the TEM images of CuO NPs synthesized using 1 : 10 ratio. HR-TEM with SAED analysis can be used to understand the crystalline characteristics and size of the synthesized nanoparticles [40]. The TEM image in Figure 5(a) reveals that the biosynthesized CuO NPs were agglomerated which are interconnected to each other and spherical in shape which is also in agreement with the result obtained from SEM. The crystalline nature of the synthesized CuO nanoparticle is confirmed by the SAED pattern which is represented by Figure 5(b). The SAED pattern indicates the polycrystalline nature of the prepared CuO NPs [41]. In general, the results obtained from morphological characterizations analysis (SEM and TEM) confirmed that the biosynthesized CuO NPs using Catha edulis leaf extract are agglomerated and spherical in shape which is also similar to the previously reported work [42].

(a)

(b)

Figure 5: (a) TEM image and (b) SAED pattern of biosynthesized CuO NPs from copper (II) nitrate trihydrate and leaf extract of Catha edulis in 1 : 10 ratio (w/v).

3.5. UV-Visible Spectra

Figures 6(a), 6(b), and 6(c) show the UV-Vis spectrum of green synthesized CuO NPs synthesized using 1 : 10, 3 : 10, and 1 : 2 ratios, respectively. The biosynthesized CuO NPs showed high absorbance over the visible wavelength range which is one of the indications that the narrow-band gap CuO NPs were successfully synthesized [35].

Figure 6: UV-V is spectra of CuO NPs synthesized using copper (II) nitrate trihydrate and Catha edulis leaf extract in (a) 1 : 10, (b) 3 : 10, and (c) 1 : 2 ratios (w/v).

As shown in Figure 7, the band gap values of the CuO NPs were determined by analyzing the optical data with the expression for the optical absorbance α and the photon energy hν using Tauc’s plot [43].

where α is the absorption coefficient, which is proportional to the absorbance, h is Planck’s constant (J · s), ν is the light frequency (s⁻¹), A is the absorbance, is the bandgap energy, and n is a constant related to the electronic interband transition.

Figure 7: Tauc plots of CuO NPs synthesized using copper (II) nitrate trihydrate and Catha edulis leaf extract in (a) 1 : 10, (b) 3 : 10, and (c) 1 : 2 ratios (w/v).

The band gaps of the CuO NPs were, then, determined by extrapolating the straight line portion of the versus graphs to the axis until . Based on Tauc’s plot, the bandgaps of synthesized CuO NPs with ratios 1 : 10, 3 : 10, and 1 : 2 were obtained to be 1.31, 1.39, and 1.45 eV, respectively. These findings are similar with previous reports made on CuO nanoparticles [35].

3.6. Fourier Transform Infrared Spectroscopy

Figures 8(a) and 8(b) show the FTIR spectra of as-synthesized and calcinated CuO NPs. Based on the FTIR spectrum of CuO NPs, the most significant absorption peaks were those observed at 531 and 603 cm⁻¹ that correspond to the stretching vibration of the Cu-O bond in monoclinic CuO which is in close agreement with the previously reported work [20]. The strong peaks at 1030 and 1053 cm⁻¹ were due to C-O stretching vibration in the carboxylic group and flavanones. The characterized peaks observed at 1383 cm⁻¹ were due to C-N stretching vibration in the amine group [44]. The strong bands at 1604 and 1638 cm⁻¹ were due to aromatic C=C bending vibration, and absorption peaks at 2875 cm⁻¹ and 2884 cm⁻¹ were attributed to the asymmetric and symmetric C-H stretching mode caused by the phenolic compounds [24]. The broad band at 3439 cm⁻¹ was due to O-H stretching vibration in alcohols. The presence of these biologically active plant compounds might have been responsible for the reduction and capping of CuO NPs. These results were similar to the previous report on the synthesis of CuO NPs using different plant extracts [7, 17].

Figure 8: FTIR spectra of (a) calcinated and (b) as-synthesized CuO NPs synthesized from copper (II) nitrate trihydrate and leaf extract of Catha edulis in 1 : 10 ratio (w/v).

3.7. Antibacterial Activity

The antibacterial activity of the biosynthesized CuO NPs was determined by using the disk diffusion method against Gram-positive and Gram-negative bacterial strains. Table 1 shows antibacterial activities of 20 mg/mL and 40 mg/mL of biosynthesized CuO NPs against the selected bacterial strains S. aureus, S. pyogenes, E. coli, and K. pneumonia. Each experiment was repeated three times, and the resulting bacterial growths on three plates corresponding to a particular sample were reported as the mean ± standard deviation (n = 3). CuO NPs synthesized using 1 : 2 ratios for both concentrations used showed high antibacterial activity against all bacterial strains compared to CuO NPs synthesized using 1 : 10 and 3 : 10 ratios. This is most probably due to the small size it possessed compared to others that led to high penetration ability and causing fast cell damage. Furthermore, as the concentration of the NPs increased, the antibacterial activity was also improved that agrees with the previous report [45].

Table 1: Antibacterial activity of CuO NPs on selected pathogenic bacterial strains.

Treatment
S. aureus
S. pyogenes
E. coli
K. pneumonia

CuO 1 : 10 ratio (20 mg/mL)
6 ± 0.02
6 ± 0.02
9 ± 0.03
8 ± 0.01

CuO 3 : 10 ratio (20 mg/mL)
7 ± 0.04
6 ± 0
10 ± 0.02
9 ± 0.04

CuO 1 : 2 ratio (20 mg/mL)
9 ± 0.01
9 ± 0.02
12 ± 0.05
11 ± 0.03

CuO 1 : 10 ratio (40 mg/mL)
16 ± 0.04
18 ± 0.01
21 ± 0.03
19 ± 0.01

CuO 3 : 10 ratio (40 mg/mL)
18 ± 0
20 ± 0.03
29 ± 0.02
28 ± 0.04

CuO 1 : 2 ratio (40 mg/mL)
22 ± 0.01
24 ± 0.02
32 ± 0.02
29 ± 0.03

Catha edulis
17
29
22
16

Gentamicin (+ve control)
22
22
22
22

DMSO (−ve control)
—
—
—
—

The findings of this study also revealed that Gram-positive bacterial strains were less sensitive than Gram-negative strains against the biosynthesized CuO NPs. The opposite charges that exist on bacterial strains and copper ions released from NPs are thought to cause adhesion and enhance bioactivity due to electrostatic forces. As a result, since peptidoglycans are negatively charged molecules, they bind with Cu²⁺ ions released from CuO NPs in a liquid growth medium. Another proposed mechanism is that copper ions released from the NPs may attach to the negatively charged bacterial cell wall and rupture it leading to protein denaturation and cause cell death. The Gram-negative bacterial strains, E. coli and K. pneumoni, may allow more Cu²⁺ ions to reach the plasma membrane. However, the previous report showed that Gram-negative bacterial strains (E. coli and K. pneumonia) were found less susceptible to antibiotics and antibacterial agents than Gram-positive bacteria (S. aureus and S. pyogen) [46, 47]. Since NPs may be smaller in size than bacterial pores, the NPs could have a unique ability to penetrate the cell membrane and cause cell death of the bacterial strains which may be the case for CuO NPs [1, 48]. The antimicrobial activities of Catha edulis extract obtained from the aqueous solution against the selected bacterial species were also tested. The zones of inhibitions measured against this aqueous extract were 17 mm, 29 mm, 22 mm, and 16 mm for S. aureus, S. pyogenes, E. coli, and K. pneumonia, respectively. The result showed that Catha edulis extracted were more active against the positive bacterial strain, S. pyogenes [49].

4. Conclusions

Biosynthesis of CuO NPs using Catha edulis leaves extract and copper (II) nitrate trihydrate in an aqueous medium, characterizations of the synthesized NPs, and their bioassay against the selected drug resistant bacterial strains were successfully carried out. The biosynthesized CuO NPs were extensively characterized using different characterization techniques. The morphological features of the biosynthesized CuO NPs were characterized by SEM and TEM. According to these morphological analyses, the biosynthesized CuO NPs consisted of uniformly distributed spherical-shaped particles in aggregated form. The crystallinity and crystal structure of the synthesized NPs were characterized using the XRD technique. According to the findings from XRD, the biosynthesized CuO NPs were highly crystalline and were found to be pure. The crystal size of the synthesized CuO NPs was also determined from the XRD pattern using the Scherrer equation and was found to be 28.10 nm, 25.30 nm, and 18.20 nm for 1 : 10, 3 : 10, and 1 : 2 ratios, respectively confirming that nanosized particles were synthesized. The antibacterial activities of the synthesized CuO NPs against Gram-negative (K. pneumonia and E. coli) and Gram-positive (S. aureus and S. pyogenes) bacterial strains were evaluated by measuring the zone of inhibition and were found to be promising against the selected bacterial strains. Accordingly, CuO NPs synthesized in 1 : 2 ratios with smaller crystal size (18.2 nm) showed high antibacterial activities against all bacterial strains, and the zone of inhibitions were found to be 22 mm, 24 mm, 32 mm, and 29 mm against bacterial strains K. pneumonia, E.coli, S. aures, and S. pyogenes, respectively.

Data Availability

The data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

Acknowledgments

The authors acknowledge Adama Science and Technology University (ASTU) for supporting this project through ASTU’s 12 cycle research grant successfully. The authors extend their thanks to the Department of Applied Chemistry and Department of Materials Science and Engineering at Adama Science and Technology University for providing facilities such as the UV-Visible spectrophotometer, thermal Analyzer, and XRD. The Department of Chemistry at Addis Ababa University is also thanked by the authors for FTIR characterization. Furthermore, the authors duly acknowledge National Twain Science and Technology University, Department of Materials Science and Engineering, Twain, for SEM, EDX, and TEM data generation. This research work was supported by Adama Science and Technology University through its 12th cycle research grant. The funder, Adama Science Technology University, has not been involved in editing, approval, or decision to publish this manuscript. The authors declare that the funder has no issue of conflicts of interest with authors concerning submission and publication of this research work.

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