Journal of Pregnancy
Volume 2020 (2020), Article ID 5658327, 6 pages
https://doi.org/10.1155/2020/5658327

Research Article

Respiratory Distress Syndrome in Infants Delivered via Cesarean from Mothers with Preterm Premature Rupture of Membranes: A Propensity Score Analysis

Mariko Nakahara,¹ Shunji Goto,² Eiji Kato,² Atsuo Itakura,¹ and Satoru Takeda¹

¹Department of Obstetrics and Gynecology, Juntendo University Faculty of Medicine, Bunkyo-ku, Tokyo, Japan
²Perinatal Center for Maternity and Neonate, Japan Community Health Care Organization Funabashi Central Hospital, Funabashi, Chiba, Japan

Correspondence should be addressed to Mariko Nakahara; mrnakaha@juntendo.ac.jp

Received 28 February 2020; Revised 16 June 2020; Accepted 23 June 2020; Published 31 July 2020

Academic Editor: Olav Lapaire

Copyright © 2020 Mariko Nakahara et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Objective. This study aimed to clarify the effects of cesarean delivery on neonatal respiratory morbidity when women had preterm premature rupture of membranes. Methods. This retrospective study included women with preterm premature rupture of membranes who delivered from 23 weeks to 33 weeks of gestation between January 2009 and December 2014. Neonatal outcomes were compared between infants delivered by cesarean section and those delivered vaginally. The primary outcome was respiratory distress syndrome (RDS). Neonatal intubation and mechanical ventilation periods were secondary outcomes. Propensity score matching was used to compare outcomes between cesarean and vaginal delivery cases. Results. There were 101 cesarean deliveries and 89 vaginal deliveries. A comparison of the presence or absence of neonatal complications based on the delivery type indicated a higher occurrence of RDS with cesarean deliveries (). The intubation and mechanical ventilation periods were not significantly longer in neonates delivered via cesarean section. Conclusions. Cesarean delivery is a risk factor for neonatal RDS in women with preterm premature rupture of membranes. Trials identifying long-term neonatal prognoses are needed to further develop optimal management strategies in such cases.

1. Introduction

Preterm premature rupture of membranes (PPROM) occurs in 3% of all pregnancies [1]. Respiratory distress syndrome (RDS) is reportedly the most common and serious complication following preterm birth [2], and it is known to be a risk factor for cerebral palsy [3]. Zanardo et al. [4] reported that RDS occurred in 55.4% of newborns born to women with PPROM. Cesarean delivery (CD) is a risk factor for RDS in preterm neonates; however, other risk factors exist, such as low birth weight [5], maternal multiparity, male sex [6], and prepregnancy body mass index (BMI) [7].

Treatment with prenatal corticosteroids, the onset of labor, and histological chorioamnionitis have been shown to decrease the incidence of RDS [8–11]. Although a randomized trial would be the ideal research method for determining whether CD is a risk factor for RDS, such a trial would require the selection of optimal cases of CD based on clinical practice. Therefore, a randomized trial is not possible. However, propensity scores, introduced by Rosenbaum and Rubin in 1983, could be used to circumvent these covariate restrictions [12]. This score could be used to create groups that differ according to treatment exposure but have otherwise similar baseline characteristics, thereby allowing randomization [13]. In this study, we performed propensity score matching analyses with the aim of clarifying the effects of CD on neonatal respiratory morbidity.

2. Materials and Methods

This retrospective study included singleton pregnancies with PPROM delivered between weeks 24 and 33 of gestation, from January 2009 to December 2014, at Japan Community Healthcare Organization Funabashi Central Hospital. Those with maternal malignancies and fetal chromosome abnormalities were excluded. The Institutional Review Committee of Japan Community Healthcare Organization Funabashi Central Hospital approved this study (March 22, 2018; no. H 30-5). Informed consent was obtained from all patients. This study was conducted in accordance with the Declaration of Helsinki.

Neonatal outcomes were compared based on the type of delivery (CD or vaginal). The primary outcome was the occurrence of RDS, which was defined as the number of neonates who developed RDS. Secondary outcomes were neonatal intubation and mechanical ventilation. PROM (premature rupture of membranes) was defined as amniotic fluid leakage (defined as classical PROM) or a clear positive immunochromatographic dipstick test for insulin-like growth factor-binding protein [1]. Before analyzing amniotic fluid secreted before the onset of labor, a high PROM threshold value was set at 25 μg/L (sensitivity, 95.2%; specificity, 90.5%) [14].

Our PPROM strategy, which was expectant management until 35 weeks, included vital sign and laboratory data monitoring for infection or inflammation. If a patient was not experiencing labor, then our management included 12 mg dexamethasone every 24 h for up to 2 days to improve fetal lung maturity. In cases of spontaneous onset of labor, nonreassuring fetal status, suspected abruptio placentae, or clinical chorioamnionitis, delivery was started immediately. CD was performed, especially in cases of a nonreassuring fetal status and suspected abruptio placentae. For clinical chorioamnionitis, if spontaneous delivery with labor was not likely to occur within a short period of time, then CD was performed. Clinical chorioamnionitis was diagnosed based on maternal fever, blood test results, presence of discharge, abdominal pain, and fetal cardiography results (Lencki criteria) [15].

Peripartum maternal factors, delivery information, and neonatal outcomes were extracted from medical records. Maternal prepregnancy BMI was calculated from the prepregnancy weight and height as follows: . Body temperature was measured in degrees Celsius. Maternal and fetal well-being were frequently observed until delivery, and venous blood examinations, including white blood cell counts and C-reactive protein (CRP) levels, were performed every few days. Patients whose last examination was conducted within 24 h before parturition were included in this study. Labor was defined as painful contractions occurring more frequently and regularly than once every 5 min, as confirmed subjectively or with cardiotocography; this information was extracted from the medical records. Fetal distress was defined as a significant abnormality in the fetal heart rate. The PROM latency period was defined as the number of days from membrane rupture to delivery. Maternal diabetes was divided into gestational diabetes mellitus (GDM) and overt diabetes. GDM was determined by performing a 75-g oral glucose tolerance test (OGTT). The diagnostic criteria for GDM is a fasting plasma glucose (FPG) >92 mg/dl and/or 1-h plasma glucose 180 mg/dl, or a 2-h plasma glucose >153 mg/dl. Criteria for overt diabetes is an FPG ≥126 mg/dl, HbA1C ≥6.5%, or a random plasma glucose ≥200 mg/dl. One of these criteria must be met to identify the patient as having overt diabetes in pregnancy [16]. The patients who delivered within the therapeutic window for antenatal corticosteroids were defined as women who delivered within 2-7 days of receiving the initial dose [17].

The delivery route was classified as vaginal delivery or CD. Diagnosis of RDS was based on characteristic clinical signs, such as tachypnea, retractions, nasal flaring, grunting, cyanosis, and chest X-ray findings of a reticulogranular pattern. Severe neonatal morbidity was defined as intraventricular hemorrhage [18] or cystic periventricular leukomalacia (i.e., periventricular white matter echolucencies on ultrasonography) [19]. Neonatal sepsis was defined as culture-proven sepsis.

Statistical analyses were performed using R version 3.4.1 (R Foundation, Vienna, Austria). Differences were considered statistically significant at a confidence level of (two-sided alternative hypothesis).

Demographic and clinical data are presented as frequency distributions and percentages. Continuous variables are expressed as the median and interquartile range. Differences in outcomes between CD and vaginal delivery were calculated using the test or Fisher’s exact test for categorical variables, and the -test or Mann-Whitney test for continuous variables. To reduce the effects of treatment selection bias and potential confounding in this observational study, differences in the baseline characteristics of patients were adjusted using propensity score matching. The predicted probability of CD was calculated by running a logistic regression model using all clinically relevant variables. The subject pairs, who delivered via vaginal delivery or CD, were derived using 1 : 1 nearest-neighbor matching with a ±0.05 caliper and no replacement.

After propensity score matching was performed, differences between the two groups were assessed using the paired -test for continuous variables and the McNemar test for categorical variables. Univariate and multivariate logistic regression analyses were performed to determine the risk factors for RDS. The risk factors examined were maternal age, multiparity, prepregnancy BMI, antepartum maternal CRP level, antenatal corticosteroid use, delivery week, and CD.

3. Results and Discussion

During the study period, 204 singleton pregnancies met our inclusion criteria. Of these, we excluded 14 (fetal trisomy 21, ; maternal malignancy, ; multiple malformations, ; neonatal death, ; no examination within 24 h before parturition, ). Therefore, 190 women and neonates were analyzed (Figure 1). The median gestational age (GA) at PROM was 30 weeks (interquartile range, 19–33 weeks), and the median GA at delivery was also 30 weeks (interquartile range, 24–33 weeks). Ten women (5.3%) were diagnosed with GDM and 2 (1.0%) were diagnosed with overt diabetes. The number of women who were administered corticosteroids during pregnancy was 103 (54.2%), and the number of women who delivered within the therapeutic window for antenatal corticosteroids was 44 (23.2%).

Figure 1: Flow chart of the included participants. PPROM: preterm premature rupture of membranes.

Matching, using the estimated propensity score, created a matched cohort of 104 patients (52 patients in each group). Patient characteristics before and after matching are listed in Table 1. Prior to matching, there were more multiparous women and the prepregnancy BMI was significantly higher in the vaginal delivery group than in the CD group ( and , respectively). The GA at PROM and GA at delivery were earlier and the neonatal birth weight was lower in the CD group than in the vaginal delivery group (, , and , respectively). Suspected abruptio placentae occurred more frequently in the CD group than in the vaginal delivery group (). However, almost all initially observed differences were balanced in the groups after matching (Figure 2). Neonatal outcomes before and after matching are shown in Table 2. Prior to matching, the incidence of RDS was significantly higher in the CD group than in the vaginal delivery group (). The intubation and mechanical ventilation periods were significantly longer in neonates delivered via CD than in those delivered vaginally ( and , respectively). There were no differences in the incidence of intraventricular hemorrhage between groups (). After matching, the incidence of RDS was still higher in neonates delivered via CD than in neonates delivered vaginally; although, the intubation and mechanical ventilation periods were no longer different between the two groups (, , and , respectively).

Table 1: Baseline patient characteristics before and after propensity score matching.

Characteristic
Before matching
After matching

Cesarean delivery
Vaginal delivery
value
SMD
Cesarean delivery
Vaginal delivery
value
SMD

Patients,
101
89

51
51

Maternal age, years
33 [29, 36]
33 [29, 36]
0.904
0.465
33 [31, 36]
34 [30.5, 36]
0.806
2.468

Multiparous
63 (62.4)
38 (42.7)
0.009
40.198
26 (51)
27 (52.9)
1.000
3.925

Prepregnancy BMI, kg/m²
19.95 [18.74, 22.36]
21.11 [19.56, 23.80]
0.023
22.295
20.61 [18.85, 22.85]
20.96 [19.52, 23.74]
0.432
4.281

GA at PROM, weeks
29 [26, 31]
30 [28, 32]
0.001
48.997
30 [28, 32]
30 [27, 32]
0.938
4.545

GA at delivery, weeks
30 [27, 31]
32 [30, 32]
<0.001
61.823
30 [29, 32]
30 [28, 32]
0.981
1.546

Patients who received antenatal corticosteroid, , (%)
55 (54.5)
48 (53.9)
1
1.049
24 (47.1)
32 (62.7)
0.163
31.923

Antepartum CRP, mg/dL
1.29 [0.24, 3.00]
0.79 [0.31, 2.14]
0.426
25.252
1.02 [0.38, 2.19]
0.84 [0.21, 2.13]
0.359
8.867

Suspected abruptio placentae, (%)
20 (19.8)
1 (1.1)
<0.001
60.082
2 (3.9)
1 (2.0)
1.000
11.625

Neonatal birth weight, g
1319 [936, 1667]
1692 [1357, 1901]
<0.001
58.239
1523 [1182, 1897.5]
1521 [1199, 1792.5]
0.862
1.72

Neonatal male sex, (%)
55 (54.5)
50 (56.2)
0.884
3.469
30 (58.8)
27 (52.9)
0.690
11.868

Values are presented as numbers (%) or medians [interquartile range]. SMD: standardized mean difference; BMI: body mass index; GA: gestational age; PROM: premature rupture of membranes; CRP: C-reactive protein.

Figure 2: Standardized differences plot before and after propensity score matching.

Table 2: Neonatal outcomes before and after matching.

Variable
Before matching
After matching

Cesarean delivery
Vaginal delivery
value
Cesarean delivery
Vaginal delivery
value

Patients,
101
89

51
51

RDS,
55 (54.5)
20 (22.5)
<0.001
26 (51.0)
14 (27.5)
0.025

Intubation period, days
2 [0, 21]
0 [0, 1]
<0.001
1 [0, 7]
0 [0, 2]
0.144

Mechanical ventilation period, days
15 [2, 50]
2 [0, 11]
<0.001
5 [1, 29]
1 [0, 29]
0.18

IVH,
5 (5.0)
2 (2.2)
0.451
3 (5.9)
2 (3.9)
0.678

Neonatal sepsis,
4 (4.0)
5 (5.6)
0.735
2 (3.9)
3 (5.9)
1.000

Values are presented as numbers (%) or medians [interquartile range]. RDS, respiratory distress syndrome; IVH, intraventricular hemorrhage.

Our propensity score matching analysis study yielded several important findings. First, we found that delivery via CD increased the risk of RDS in neonates when the mothers had PPROM. A previous meta-analysis also showed that the pooled odds ratio of neonatal RDS associated with CD was 1.76 (95% confidence interval, 1.48–2.09), and both elective CD and emergency CD were associated with an increased risk of neonatal RDS [20]. An epidemiological study on neonatal respiratory diseases in Sweden also reported that acute respiratory morbidity for moderately preterm infants is common and predicted based on multiparity, CD, low Apgar score, and male sex [21]. When determining whether CD increases the risk of RDS, we need to consider the GA, obesity, antenatal corticosteroid use, and any other factors that may affect the occurrence of RDS [6]. In this study, we reduced these biases by using propensity score matching and revealed that CD was still a significant risk factor for RDS.

After performing a propensity score matching analysis, we determined that the mechanical ventilation and intubation periods of newborns delivered by women with PPROM via CD were not significantly longer than those of newborns delivered vaginally. Although mechanical ventilation is a life-saving intervention for premature infants, a longer cumulative duration of mechanical ventilation is associated with increased hospitalization and an increase in the required duration of parenteral nutrition, as well as a higher probability of discharge with poor achievement of physical growth [22]. Although CD should be avoided in women with PPROM to prevent RDS, our study did not show evidence of an association between CD and the duration of ventilation after bias was removed.

This study has a few limitations. The main limitations were that it was nonprospective and nonrandomized. Although a propensity score matching analysis was performed to overcome the weaknesses of retrospective case studies, by reducing bias with estimates resulting from observed differences between CD and vaginal delivery, the study was still subject to bias from unobserved differences. Another limitation was that we could not exclude the effects of labor as a bias, because labor during vaginal delivery was stronger than that during CD. Generally, labor prevents neonatal RDS. The Maternal Fetal Medicine Units Network supports previously published data indicating a higher occurrence of respiratory distress in infants delivered via elective repeat CD than in those delivered via vaginal birth after CD [23]. As hormones such as catecholamines and corticosteroids are produced during labor, the fetal lungs begin producing surfactants. Notably, labor results in the switch from active chloride and fluid secretion to active sodium and fluid absorption in fetal lungs [24]. In this study, whether labor had started was not included as a covariate. Prospective studies that assess factors such as labor timing are needed to reduce bias regarding whether neonatal outcomes change depending on the delivery type (i.e., vaginal delivery or CD) after labor has begun.

In conclusion, CD is a risk factor for neonatal RDS in women with PPROM. Thus, CD should be avoided in women with preterm PROM. Prospective and randomized trials to identify the long-term neonatal prognoses are needed to establish optimal management approaches for PPROM.

Data Availability

The data used to support the results of this study are available from the corresponding author upon reasonable request in order to protect patients’ personal data.

Conflicts of Interest

The authors declare that there is no conflict of interest regarding the publication of this article.

Authors’ Contributions

M. N. collected and analyzed the data. All authors contributed to the writing of the manuscript.

References

T. P. Waters and B. Mercer, “Preterm PROM,” Clinical Obstetrics and Gynecology, vol. 54, no. 2, pp. 307–312, 2011.

J. A. Lemons, C. R. Bauer, W. Oh et al., “Very low birth weight outcomes of the National Institute of Child health and human development neonatal research network, January 1995 through December 1996,” Pediatrics, vol. 107, no. 1, p. e1, 2001.

S. K. Thygesen, M. Olsen, J. R. Østergaard, and H. T. Sørensen, “Respiratory distress syndrome in moderately late and late preterm infants and risk of cerebral palsy: a population-based cohort study,” BMJ Open, vol. 6, no. 10, article e011643, 2016.

V. Zanardo, S. Vedovato, E. Cosmi et al., “Preterm premature rupture of membranes, chorioamnion inflammatory scores and neonatal respiratory outcome,” BJOG, vol. 117, no. 1, pp. 94–98, 2010.

V. Condò, S. Cipriani, M. Colnaghi et al., “Neonatal respiratory distress syndrome: are risk factors the same in preterm and term infants?” The Journal of Maternal-Fetal & Neonatal Medicine, vol. 30, no. 11, pp. 1267–1272, 2016.

M. Altman, M. Vanpée, S. Cnattingius, and M. Norman, “Risk factors for acute respiratory morbidity in moderately preterm infants,” Paediatric and Perinatal Epidemiology, vol. 27, no. 2, pp. 172–181, 2013.

A. M. Faucett, T. D. Metz, P. E. DeWitt, and R. S. Gibbs, “Effect of obesity on neonatal outcomes in pregnancies with preterm premature rupture of membranes,” American Journal of Obstetrics and Gynecology, vol. 214, no. 2, pp. 287.e1–287.e5, 2016.

K. Shimoya, T. Taniguchi, N. Matsuzaki et al., “Chorioamnionitis decreased incidence of respiratory distress syndrome by elevating fetal interleukin-6 serum concentration,” Human Reproduction, vol. 15, no. 10, pp. 2234–2240, 2000.

H. Moore and V. Venugopalan, “Antenatal betamethasone prevented respiratory distress syndrome in late preterm infants,” Archives of disease in childhood - Education & Practice Edition, vol. 103, no. 4, p. 218, 2018.

G. C. Liggins and R. N. Howie, “A controlled trial of antepartum glucocorticoid treatment for prevention of the respiratory distress syndrome in premature infants,” Pediatrics, vol. 50, no. 4, pp. 515–525, 1972.

T. Shimokaze, K. Akaba, M. Banzai, K. Kihara, E. Saito, and H. Kanasugi, “Premature rupture of membranes and neonatal respiratory morbidity at 32-41 weeks’ gestation: A retrospective single-center cohort study,” The Journal of Obstetrics and Gynaecology Research, vol. 41, no. 8, pp. 1193–1200, 2015.

P. R. Rosenbaum and D. B. Rubin, “The central role of the propensity score in observational studies for causal effects,” Biometrika, vol. 70, no. 1, pp. 41–55, 1983.

D. B. Rubin, “On principles for modeling propensity scores in medical research,” Pharmacoepidemiology and Drug Safety, vol. 13, no. 12, pp. 855–857, 2004.

T. Kubota and H. Takeuchi, “Evaluation of insulin-like growth factor binding protein-1 as a diagnostic tool for rupture of the membranes,” The Journal of Obstetrics and Gynaecology Research, vol. 24, no. 6, pp. 411–417, 1998.

S. G. Lencki, M. B. Maciulla, and G. S. Eglinton, “Maternal and umbilical cord serum interleukin levels in preterm labor with clinical chorioamnionitis,” American Journal of Obstetrics and Gynecology, vol. 170, no. 5, pp. 1345–1351, 1994.

International Association of Diabetes and Pregnancy Study Groups Consensus Panel, “International Association of Diabetes and Pregnancy Study Groups Recommendations on the diagnosis and classification of hyperglycemia in pregnancy,” Diabetes Care, vol. 33, no. 3, pp. 676–682, 2010.

Committee on Obstetric Practice, “Committee opinion no. 713,” Obstetrics & Gynecology, vol. 130, no. 2, pp. e102–e109, 2017.

L. A. Papile, J. Burstein, R. Burstein, and H. Koffler, “Incidence and evolution of subependymal and intraventricular hemorrhage: a study of infants with birth weights less than 1,500 gm,” The Journal of Pediatrics, vol. 92, no. 4, pp. 529–534, 1978.

J. J. Volpe, “Brain injury in premature infants: a complex amalgam of destructive and developmental disturbances,” Lancet Neurology, vol. 8, no. 1, pp. 110–124, 2009.

Y. Li, C. Zhang, and D. Zhang, “Cesarean section and the risk of neonatal respiratory distress syndrome: a meta-analysis,” Archives of Gynecology and Obstetrics, vol. 300, no. 3, pp. 503–517, 2019.

M. Roth-Kleiner, B. P. Wagner, D. Bachmann, and J. Pfenninger, “Respiratory distress syndrome in near-term babies after caesarean section,” Swiss Medical Weekly, vol. 133, no. 19-20, pp. 283–288, 2003.

Y. B. Choi, J. Lee, J. Park, and Y. H. Jun, “Impact of prolonged mechanical ventilation in very low birth weight infants: results from a national cohort study,” The Journal of Pediatrics, vol. 194, pp. 34–39.e3, 2018.

L. Jain and D. C. Eaton, “Physiology of fetal lung fluid clearance and the effect of labor,” Seminars in Perinatology, vol. 30, no. 1, pp. 34–43, 2006.

A. Ramachandrappa and L. Jain, “Elective cesarean section: its impact on neonatal respiratory outcome,” Clinics in Perinatology, vol. 35, no. 2, pp. 373–393, 2008.