Thymoquinone β-Cyclodextrin Nanoparticles System: A Preliminary Study

T. Cardoso,1 C. I. C. Galhano,2, 3 M. F. Ferreira Marques,4, 5 and A. Moreira da Silva1, 6 1Department of Food Science and Technology, Coimbra College of Agriculture, Bencanta, 3040-316 Coimbra, Portugal 2Department of Environment, Coimbra College of Agriculture, Bencanta, 3040-316 Coimbra, Portugal 3CERNAS Research Unit, Bencanta, 3040-316 Coimbra, Portugal 4Department of Chemical and Biological Engineering, ISEC-IPC, 3031-199 Coimbra, Portugal 5CEMUC, Department of Physics, University of Coimbra, 3004-516 Coimbra, Portugal 6Research Unit Molecular Physical-Chemistry, University of Coimbra, 3000-535 Coimbra, Portugal


Introduction
Thymoquinone (TQ) is the main constituent of Nigella sativa seeds, commonly known as black seeds.The Nigella sativa essential oil has been traditionally employed in folk medicine due to various pharmacological effects.
Previous studies reported that TQ exhibits strong antioxidant, anti-inflammatory, antineoplastic, and analgesic effects, both in vitro and in vivo.
There is a growing interest in the therapeutic potential of TQ in different research fields, including diabetes, but, particularly, in cancer therapy.TQ was found to be a potent inhibitory drug in colon cancer cell, leukemia cells, laryngeal carcinoma cells, pancreatic cells, ovarian adenocarcinoma, uterine sarcoma, and prostate cancer cells, while it is minimally toxic to non-neoplastic cells.Additionally, TQ and Nigella sativa are also promising chemopreventive agents [1].
Most drugs, especially those of hydrophobic nature, have failed in human clinical trials due to either lack of safety or poor efficacy, which may in part be a consequence of their poor bioavailability [2].Although natural products have served as leads for the majority of clinically used drugs, poor oral bioavailability has greatly hindered their development.
The inclusion of molecules into cyclodextrin (CD) cavities has been intensively used in pharmaceutical, food, and cosmetic industries, in order to produce more stable preparations with improved hydrophilicity [3].
CDs are cyclic oligosaccharides, macrocycles with a hydrophilic external surface and a hydrophobic internal moiety, consisting of six (α-CD), seven (β-CD), or eight (γ-CD) D-glucopyranose residues linked by α-1,4 glycosidic bonds, that can be represented as a truncated cone structure (Figure 1).Their inner cavity diameters are about 0.57, 0.78, and 0.95 nm, respectively.As drug carriers, the fundamental advantages of natural cyclodextrins are (i) their complete known chemical structure, with several potential sites for chemical modification or conjugation; (ii) the availability of different cavity sizes; (iii) their low toxicity and pharmacological activity; (iv) their significant water solubility; (v) the protection of included/conjugated drugs from biodegradation [4].
The possible guest list for molecule encapsulation in cyclodextrins includes compounds such as straight or branched chain hydrocarbons, aldehydes, ketones, alcohols, organic acids, fatty acids, aromatic compounds, gases (e.g., 1-methylcyclopropene), and polar compounds such as halogens, oxyacids, and amines [5].The hydrophobic cavity of cyclodextrins provides an environment into which appropriately sized nonpolar molecules can enter and form stable inclusion species.During inclusion no covalent bonds are broken or formed [6].One of the most important applications of cyclodextrins in the pharmaceutical field is the enhancement of the aqueous solubility of drugs through CD inclusion (nanoencapsulation).

Objectives
To study the properties of the inclusion compounds based on cyclodextrins (Figure 1) and thymoquinone (TQ) (Figure 2), in both aqueous medium and the solid state, using Fourier Transform Infrared (FTIR), Ultraviolet-Visible (UV-vis), and Positron Annihilation Lifetime (PAL) Spectroscopies, as well as Differential Scanning Calorimetry (DSC) experiments.

Material and Methods
β-CD was a gift from Wacker-Chemie and TQ was purchased from Sigma.The inclusion compounds were prepared by the coprecipitation method [4].
The FTIR spectral studies were carried out using a Nicolet 170SX FTIR spectrometer, employing a KBr pellet method.
The inclusion compound stoichiometry determination was carried out by the continuous variation method (Job plots [7]), followed by visible-ultraviolet spectrophotometry using the equipment Kontron Uvikon (Serial 922) at λ max = 331 nm.
Adequate samples were prepared for carrying out the PALS measurements [8].This was achieved by pressing the powders under ∼ 70 bars into disks of 10 mm diameter and about 0.5 mm thickness.
The positron 22 Na (ca.7 × 10 4 Bq, closed between Kapton foils) was sandwiched between two identical sample disks.As the thickness of the specimens was not sufficient to stop all the positrons, "backing" of SS316 stain steel was used and the set finally wrapped in Al-foil.All measurements were performed at 298 K with the sample assembly placed into an evacuated stainless steel tube.
The lifetime (LT) spectra were recorded in our fast-fast coincidence PALS setup (featuring Pilot-U scintillators and XP 2020 photomultipliers) with a time resolution of 270 ps [8].Each sample was measured four times with individual lifetime spectra having ca.2.5×106 integral counts and the analysis was performed with the LT (version 9) code [9].
The Differential Scanning Calorimetry (DSC) thermograms were performed by a Mettler TA 4000 apparatus equipped with a DSC 25 cell.Samples were weighted in aluminium pans with a perforated lid and scanned at 10 • C min −1 between 30 • C and 300 • C.

FTIR Studies
The FTIR spectrum of the thymoquinone @ β-CD inclusion compound, in the crystalline state, was analysed (Figure 3).The thymoquinone molecule (Figure 2) contains vibrational bands (namely, in FTIR) frequencies quite suitable for probing the guest perturbation by either mixing or inclusion with CD's.In particular, the infrared (IR) bands in 1560-1800 cm −1 region were found to be good structural probes (Figure 3).
The infrared spectrum of the inclusion compound (Figure 3) reflects a substantial variation of bond strength and length.The most interesting signals are those due to the polar functional groups of thymoquinone and β-CD, suggesting that the polar interaction can act as a stabilizing force in the inclusion compound formation.The quite complete disappearance of the thymoquinone signals is also a confirmation of the inclusion compound formation.In fact, a "shield" effect on the guest molecule by cyclodextrins has been detected for some CD inclusion compounds by NMR spectroscopy.Probably this is due to a dipole moment variation upon inclusion, that can be explained considering that the original crystalline host molecule has been quite completely rearranged in the inclusion complex [10,11].

Stoichiometry of the Host-Guest System: Job Plots
Visible-ultraviolet spectroscopic studies were used to apply the continuous variation method (Job Plots) [7] to the thymoquinone and β-CD inclusion systems.) leads to maxima at r ≈ 0.5 (Figure 4), pointing to a 1 : 1 stoichiometry [7].In fact, these distributions are exactly symmetrical, suggesting the presence of associations with a single stoichiometry, in this case, of the 1 : 1 type.

Positron Annihilation Lifetime Spectroscopy (PALS)
Figure 5 represents the observed lifetimes (τ 3 ) and intensities (I 3 ) for various samples, including pure β-CD as reference and the resulting radii according to the semiempirical model of Tao-Eldrup [8] that establishes the correlation between free-volume hole radius R (in Å) and o-Ps pick-off lifetime, τ 3 .Values for pure β-CD are in agreement with previous results [9].For the thymoquinone inclusion compound, τ 3 (and thus R 3 ) decreases with increasing thymoquinone concentration.However, for the 1 : 0.5 and 1 : 0.75 guest : host ratios there is a slight decrease, in contrast to the decrease obtained in 1 : 1.The common behavior of I 3 agrees well with the reduction of free volumes in the hollow truncated cone of the β-CD due to the inclusion of guest molecules, which decreases drastically with an increasing guest concentration.The results show that cyclodextrin molecule has few free volumes, just 0.41%, for 1 : 1 concentration which means that almost all sites are occupied.

Calorimetric Studies
As evidenced from Figure 6, DSC shows the presence of a true inclusion compound, as the thymoquinone melting band (a) disappears on the thermogram.

Conclusions
The reported experimental results indicate the formation of 1 : 1 cyclodextrin inclusion compounds with thymoquinone.Regarding the FTIR spectrum of the inclusion compound, it is very similar to that of pure β-CD.The final crystal structure of this species should be at least similar to that of β-CD, implying that TQ would be included and not interposed between β-CD molecules.This is, indeed, verified by the present experimental results, which is a good indication of the guest-host complex formation.
The PALS assays show a decrease of the β-CD free volumes upon an increase in the TQ molar fraction, reflecting an inclusion process between the two compounds.
Differential scanning calorimetry (DSC) measurements, in turn, provide evidence of inclusion compound such as the absence of the endothermic peak assigned to TQ melting.
Other studies are underway in our laboratory, namely, on the potential use of these systems in pharmaceutical formulations and/or on new food products formulations such as functional/novel foods.

Figure 5 :
Figure 5: PALS parameter and calculated radii of o-Ps trapping sites for complexes of β-CD and TQ where 1 represents pure β-CD.