Spectroscopic Analysis of Heterogeneous Biocatalysts for Biodiesel Production from Expired Sunflower Cooking Oil

The study characterized heterogeneous biocatalyst synthesized from sucrose, saw dust, and chicken egg shells using Fourier Transform Infrared (FTIR) spectroscopy coupled with Attenuated Total Reflectance (ATR) technique. Acidic sulphonate (–SO 3 H) groups were more visible in the spectrum generated for carbonized and sulphonated sucrose than in carbonized and sulphonated saw dust. This was highlighted further by the significantly higher conversion percentage achieved for sulphonated sucrose (62.5%) than sulphonated saw dust (46.6%) during esterification of expired sunflower oil (p = 0.05). The spectra for calcinated egg shells also showed that the most active form of calcium oxide was produced at calcination temperature of 1000C. This was confirmed in the single-step transesterification reaction in which calcium oxide generated at 1000C yielded the highest biodiesel (87.8%) from expired sunflower oil. The study further demonstrated the versatility of the FTIR technique in qualitative analysis of biodiesel and regular diesel by confirming the presence of specific characteristic peaks of diagnostic importance. These findings therefore highlight the potential of FTIR-ATR as an inexpensive, fast, and accurate diagnostic means for easy identification and characterization of different materials and products.


Introduction
Several methods are currently employed in the study of materials, including X-ray fluorescence (XRF), X-ray diffraction (XRD), and scanning electron microscopy (SEM).However, these methods have several disadvantages including high cost of analysis per sample, relatively large sample requirement, low reproducibility, and safety concerns with use of Xray equipment [1,2].Further, sample analysis using these techniques is tedious with lengthy sample preparatory steps, which makes working on large samples laborious [2].
These problems could be significantly reduced by using Fourier transform infrared spectroscopy (FTIR), a rapid, simple, and economic tool for studying materials, catalytic activity, and products from chemical reactions [3].Besides, this technique is precise and nondestructive, requires small samples, is easily reproducible, has less need for messy chemicals, and permits the qualitative and quantitative identification of components in solids and liquids [4,5].
Infrared spectroscopy may be defined as the triggering of vibrations in molecules of a sample through irradiation with infrared light, with the aim of producing a finger print of the sample with absorption peaks which correspond to bonds between the atoms that make up the molecule [6].The intensity and wavelength of radiation absorbed or transmitted can be measured, thus providing a basis for detection and quantification of functional groups and molecules [7].
The use of FTIR spectroscopy for analyzing catalytic activity is increasingly becoming popular since it can identify chemical bonds in molecules by producing their infrared spectra [8], thus being deemed suitable for verifying presence of desired functional groups in catalysts or for structure elucidation [3].Coupled with an attenuated total reflectance (ATR) accessory, the technique shortens the time of sample preparation and eases spectral reproducibility, thus making sample analysis faster.
Previous investigators have successively used FTIR spectroscopy to characterize both homogeneous and heterogeneous catalysts [9,10].For instance, Engin et al. [11] first demonstrated the effect of different calcination temperatures on heterogeneous biocatalysts from egg shells using FTIR spectroscopy and were able to identify the key functional groups involved.Another study indicated the use of FTIR-ATR spectroscopy to identify acidic catalytic -SO 3 H groups on sulfonated biochar catalyst, with great ease [12], thus illustrating the wide applicability of this method in studying catalyst activity [9].
However, few studies have demonstrated FTIR-ATR spectroscopy as a stand-alone analytical tool for characterization and selection of catalysts and characterization of reactants and products of transesterification.Moreover, there is a growing need for rapid and simple tools for analysis of fatty acid methyl esters in the production chain of biodiesel industries [5].The objective of this study was to show the ability of FTIR-ATR spectroscopy as a rapid and reproducible qualitative analytical tool of liquids and solids that could replace the more laborious official methods such as gas chromatography, X-ray fluorescence, and X-ray diffraction spectroscopy in qualitative analysis.
In this study, three heterogeneous biocatalysts synthesized from locally available materials, namely, chicken eggshells, sucrose, and saw dust, were characterized using FTIR-ATR spectroscopy in the midinfrared region to investigate their catalytic potential.Subsequently, the catalysts were employed in single-step transesterification and esterification reactions using expired sunflower oil.The biodiesel produced from the single-step transesterification and regular diesel from a local filling station were similarly analyzed using FTIR-ATR spectroscopy for diagnostic purposes as well as identification of differences between the two products.

Materials and Methods
2.1.Materials.Egg shells were collected from local egg vendors; sucrose (table sugar) was purchased from a local shop, while saw dust was outsourced from a local carpentry establishment.The saw dust was graded using a fine mesh, dried, and stored under dry conditions.Methanol (98% analytical grade) and concentrated sulphuric acid (98%) were purchased from the local chemical supplier.Off-shelf cooking sunflower oil was collected from a disposal point of a selected local supermarket in Kampala city.

Preparation of Heterogeneous Base
Catalyst.The egg shells were washed with warm distilled water to remove dirt, oven-dried at 100 ∘ C for 24 hours [7], and thereafter crushed in a motorized blender (Electromix).About 200 g of crushed shells was calcinated in a muffle furnace (Nabertherm model L5/C6) at different temperature ranges 500-1000 ∘ C for 4 hours.The calcinated material (char) was left to cool and then stored in a desiccator.

Preparation of Heterogeneous Acid
Catalysts.The acid catalysts were prepared from saw dust and sucrose according to Guo et al. [13] with slight modifications.Briefly, the saw dust and sucrose were partially carbonized in a muffle furnace at 400 ∘ C under dry nitrogen.The carbonized material (char) was washed with hot distilled water and oven-dried at 105 ∘ C for 12 hours.The resulting material was sulfonated by adding 5 mL of concentrated sulphuric acid (98%) to 5 g of the char and the mixture was placed in an oven at 150 ∘ C for 2 hours (Figure 1).The mixture was left to cool and successively washed with hot distilled water until a neutral pH of the wash water was achieved.The material was dried in a hot air oven (Memmert UFE 600) at 105 ∘ C for 48 hours, cooled in a desiccator, and stored in dry containers.

FTIR-ATR Spectroscopic
Analysis.FTIR spectra of the calcinated egg shells, solid acid catalysts, biodiesel, and regular diesel were obtained in ATR mode with a Perkin Elmer Spectrum Two spectrophotometer equipped with a Universal Attenuated Total Reflectance (UATR) accessory (Version 10.4,USA).The spectrophotometer was run on software NISO2 and different spectra were obtained in the midinfrared region.
For liquid samples, 0.1 mL of sample was spread evenly on the diamond crystal of the ATR to obtain the spectra.Each spectrum was a result of four scans done at a resolution of 4 cm −1 over a wavenumber ranging from 4000 to 450 cm −1 .For solids, the sample was ground to fine powder using a mortar and pestle and then about 0.01 g of the sample was spread on the diamond ATR crystal.One spectrum was recorded per sample using air as a reference.The diamond crystal was cleaned using a tissue soaked in pure distilled water, between successive sample runs.

Transesterification and Esterification of Sunflower Oil.
Transesterification was done according to Dehkhoda and Ellis [12] with slight modifications, using egg shells calcinated at different temperatures.Briefly, 5% (w/w) of the calcinated shell was mixed with 28 mL of methanol and heated to 40 ∘ C. The mixture was added to 88 g (100 mL) of oil and heated to 60 ∘ C with constant stirring for 120 minutes.The reaction was stopped, residual catalyst sieved off, glycerol decanted off, and biodiesel was washed with hot deionized distilled water.
Esterification was conducted using 1% (w/w) solid acid catalyst, which was mixed with 28 mL of methanol at room temperature, and added to the warm oil (50 ∘ C) and gently stirred for 180 minutes.The biodiesel yield was determined from the following equations: Weight of refined methyl esters weight of used oil ) × 100, where FFA is free fatty acids.
Calcination of egg shells at 500 ∘ C eliminated organic matter like protein strands and produced sharp bands at 1402 cm −1 , 891 cm −1 , and 712 cm −1 attributed to calcium carbonate [14].The intensity of the sharp peaks reduced as calcination temperature increased from 500 to 700 ∘ C, possibly signifying the gradual thermal decomposition of carbonate molecules and subsequent emergence of calcium oxide and calcium hydroxide [11].
At temperatures higher than 700 ∘ C, there was significant reduction in the carbonate bands, which corresponded to increased rate of decomposition of carbonates [15].The presence of diminished carbonate bands in egg shells calcinated at 800 and 900 ∘ C showed that the transformation into calcium oxide (CaO) was incomplete due to insufficient calcination temperature and time [11].
Conversely, a strong and wide peak corresponding to Ca-O bonds appears in the 500 cm −1 region for egg shells calcinated above 700 ∘ C (Figure 2(b)).At 1000 ∘ C, all the carbonate bands were eliminated due to complete desorption of carbon dioxide from the eggshells, thus producing more basic sites (CaO) that are essential for transesterification to occur [14,16,17].

Effect of Carbonization and Sulphonation on Sucrose.
FTIR spectral wave numbers of sucrose (Table 1) reveal variations in the physical structure following carbonization and sulphonation treatments (Figure 3).The spectrum for untreated sucrose (3A) showed three distinct absorption zones at 3500-3000 cm −1 , 1500-1280 cm −1 and a finger print region 1200-450 cm −1 .The absorption zone in the 3500-3000 cm −1 region corresponded to an O-H vibration and the small peaks next to it represent aliphatic C-H vibrations.The absorption zone in the 1500-1280 cm −1 region represented single-bond vibrations of O-C-H and C-C-H, while the intense stretches in the finger print region represent C-O-H and C-O-C vibrations.This spectrum (3A) is similar to that of sucrose reported in previous studies [18,19].
Subsequent partial carbonization of sucrose (Figure 3(b)) produced a change in spectrum possibly due to loss of bonds in the key regions previously identified in raw sucrose.In particular, reduction in the intensity of the O-H band in the 3500-3000 cm −1 region is likely to be a result of the dehydration process leading to loss of O-H bonds due to high carbonization temperature [20].Carbonization also produced a new peak at 1707 cm −1 , corresponding to C=O vibration [21].
The peaks at wave numbers 1590 cm −1 and 1420 cm −1 most probably correspond to C=C stretches, indicative of aromatic groups.In addition, the C-O stretches and O-H bending vibrations in the 1239-986 cm −1 region are significantly diminished probably due to heat.There is broad band also in the 3700-1800 cm −1 region that could be a result   of new hydrogen bonding interactions caused by carbonization, resulting in several oscillation modes in that region [22].Overall, partial carbonization of sucrose resulted in dehydration and aromatization reactions with subsequent diminution of all major bond peaks previously observed in uncarbonized sucrose [23].
A significant change in the spectrum of carbonized sucrose was observed upon sulphonation with concentrated sulphuric acid (Figure 3(c)).The absence of an O-H peak in the 3000-3500 region indicates total dehydration of the sucrose by the concentrated sulphuric acid [21].On the other hand, the development of an intense aliphatic peak corresponding to C-H group around the 2900 cm −1 wave region was observed.
The vibration of C=O group at wave numbers 1769 and 1758 cm −1 intensified possibly due to oxidation and esterification of the abundant O-H groups in sucrose by H 2 SO 4 .Sharp peaks corresponding to O=S=O group were also observed at 1376 and 1057 cm −1 , whereas the intense peak at 1245 cm −1 was attributed to the -SO 3 H group.The formation of such strong sharp bands indicates that sucrose can easily be carbonized, dehydrated, and sulfonated to facilitate the attachment of catalytic SO 3 H acidic groups [24].

Effect of Carbonization and Sulphonation on Saw Dust.
Saw dust on the other hand presented a similar spectrum to that observed in untreated sucrose (Figure 4).There was a broad O-H bond also in untreated saw dust (Figure 4(a)) in the 3000-3500 cm −1 region, which could be attributed to cellulose and hemicellulose [25] that are the major carbohydrates in saw dust.A weak C-H vibration was observed at the 2883 cm −1 position, confirming the presence of aliphatic groups.Unlike sucrose, a weak C=O peak was observed at the 1727 cm −1 region.The region around 1630-1233 cm −1 was characterized by weak alkene (C=C), aromatic (C=C), CH 2 , and CH groups including phenolic OH groups.The most intense peak at 1032 cm −1 could be attributed to C-O vibration.The untreated saw dust spectrum observed here was similar to those seen in previous studies [8,26].
Carbonization led to the diminution of certain bonds and intensification of others (Figure 4(b)).The most notable was the reduced intensity of the O-H vibration in the 3000-3500 cm −1 as well as diminished O-H peak at 1032 cm −1 .This reduction in intensity could be attributed to the decomposition of hemicellulose at high carbonization temperatures used in this study.The C=O peaks between 1727 cm −1 and 1590 cm −1 were intensified, indicating increased aromatization due to carbonization [27].As observed in carbonized sucrose (Figure 2(b)), a broad band in the 3700-1800 cm −1 region of carbonized saw dust is likely a result of new hydrogen bonding interactions [22].Sulphonation of the biochar did not produce significant spectral changes as expected (Figure 4(c)), and, therefore, the spectrum of sulphonated char was similar to that of carbonized saw dust, with minor differences in the 1570-1000 cm −1 region.Other than the newly emerged weak band at 1555 cm −1 , no bands could be attributed directly to acidic groups as in the case of sucrose spectrum.Other bonds identified in the spectra of raw sawdust, carbonized sawdust, and carbonized sulphonated saw dust are presented in Table 2.

Transesterification and Esterification of Expired Sunflower
Cooking Oil.Single-step transesterification using eggshell calcinated at 1000 ∘ C produced the highest yield of biodiesel (87.8%).The lowest biodiesel yield (36.6%) is attained with eggshell calcinated at 700 ∘ C (Figure 5).This confirms that calcination temperature of shells has an effect on the final yield of biodiesel attained [28].The low yield of biodiesel attained with catalyst generated at 700 ∘ C is possibly a result of incomplete conversion of CaCO 3 to CaO [16,17], as evidenced by the FTIR spectra (Figure 2).On the other hand, sulphonated sucrose produced significantly ( = 0.05) higher esterification yield (62.5%) than sulphonated saw dust (46.7%) under similar reaction conditions (Figure 6).The high catalytic activity of sulfonated sucrose is likely a result of efficient sulfonation, which could have resulted in a catalyst with higher acid density than that of sulfonated sawdust, which showed no clear evidence of sulfonation (Figures 3(c) and 4(c)).A similar study found that sulphonated sucrose had a higher acid density than all other similarly treated carbons such as starch and lignin [13].The low activity of saw dust catalyst could be due to incomplete carbonization of lignin in sawdust, leaving excess oxygen in the material which limits the number of -SO 3 H groups that could attach onto the carbonized char [13].
6 Journal of Spectroscopy

Characterization of Biodiesel and Regular
Diesel Using FTIR-ATR Spectroscopy FTIR spectra of biodiesel and expired sunflower cooking oil are presented in Figure 7 and the various absorption peaks of biodiesel are presented in Table 3.There were no significant peaks in the regions 1700-2800 cm −1 and 3000-4000 cm −1 for both expired sunflower cooking oil and biodiesel spectra [29], but both showed strong peaks between 2800 and 3000 cm −1 corresponding to C-H vibrations.Major differences between the two spectra lie in the 1500-500 cm −1 and are mainly exhibited by esters (biodiesel), as a result of C-O vibrations.The main characteristic peak for biodiesel occurs at 1245 cm −1 (C-O) and corresponds to the proportion of fatty acid methyl esters [30].Other peaks diagnostic for biodiesel were identified at positions 1742 cm −1 (C=O) and 1057 cm −1 (C-O) similar to those identified in other studies [5,31].
The spectrum for regular diesel showed some similarities to that of biodiesel, particularly in the 3000-2700 cm −1 region (Figure 8).There was also a mild C=O vibration detected in the 1750-1780 cm −1 region, unlike the sharp vibration for biodiesel around a similar position as well as a C-O ester diagnostic bond at 1246 cm −1 .In contrast, other studies showed no peak in the 1750-1780 cm −1 region for diesel [5,32].Other peaks for regular diesel are presented in Table 4.

Conclusion
This study established that FTIR-ATR spectroscopy is a quick analytical technique that can be used to confirm the presence of key functional groups on heterogeneous biocatalysts, thus helping to predict activity of catalysts.The FTIR spectra of calcinated egg shells showed gradual decomposition of calcium carbonate with subsequent emergence of calcium oxide as temperature increased.Single-step transesterification of the expired sunflower oil showed that CaO generated from egg shell at 1000 ∘ C produced the highest biodiesel yield (87.8%).The spectra for carbonized sulphonated sucrose confirmed the presence of sulphonate (-SO 3 H) groups, which make the biochar catalytically active, as confirmed by the high esterification yield of 62.5%.Conversely, the sulphonated sawdust showed no clear evidence of the presence of acidic (-SO 3 H) groups, thus explaining the low esterification conversion achieved with this catalyst.The infrared spectra for biodiesel and regular diesel further help confirm that FTIR-ATR spectroscopy can be rapidly used for final product verification as compared to more costly chromatographic techniques such as HPLC and gas chromatography.These findings therefore imply that FTIR-ATR spectroscopy may be adopted as an inexpensive alternative for quick analysis of materials and products of chemical reactions.

Table 2 :
Wave numbers of FTIR spectra and associated bonds for untreated saw dust (A), carbonized saw dust (B), and carbonized + sulphonated saw dust (C).

Table 3 :
Wave numbers responsible for different peaks of biodiesel (BD).

Table 4 :
FTIR wave numbers for different peaks of regular diesel.