Synthesis and In Vitro Antimicrobial and Anthelminthic Evaluation of Naphtholic and Phenolic Azo Dyes

The antimicrobial activity of 2-naphtholic and phenolic azo compounds was determined against seven microbial species, Staphylococcus aureus (ATCC 25923), Streptococcus pyrogenes (clinical), and Enterococcus faecalis (ATCC 29212), Salmonella typhi (clinical), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 251922), and Candida albicans (ATCC 10231), using the high-throughput spot culture growth inhibition assay (HT-SPOTi). The minimum inhibitory concentrations (MIC) were determined for the active azo dyes. All the azo compounds (A1–B4) were screened for anthelmintic activity against adult Ghanaian earthworms, Hyperiodrilus spp. As part of the systematic investigation for biological activity, all the azo compounds exhibited good antimicrobial activity against the seven human pathogenic microorganisms. All the compounds exhibited anthelminthic activity against adult Ghanaian earthworms, Hyperiodrilus spp.


Introduction
Over the past decades, the emergence of resistant pathogens in humans has been on the ascendency and has become a global health concern in chemotherapy of infectious diseases [1].
is has therefore stimulated efforts in the field of antimicrobial chemotherapy search for novel drugs to curtail this menace [2]. Bacterial resistance is an inevitable consequence of evolution, and without continued replacement of current antibacterial agents, humanity runs the risk of returning to a preantibiotic era [3]. A section of neglected tropical diseases are associated with helminths, and they are amongst the most common ones affecting the world and posing major public health threat, thus contributing to high morbidity and mortality [3,4]. e existence of few antimicrobials and anthelminthics coupled with current chemotherapy losing their efficacy calls for continuous research for new but more effective compounds in the drug discovery industry [5,6].
With regards to this assertion, azo compounds have been found to be useful, extending the scope of drug design and allowing different mechanisms of action [7,8].
e increasing interest in the development of azo compounds is due to their functions and versatile biological activities including antimicrobial, anti-inflammatory, anthelminthic, antiviral, and anticancer effects [9,10]. Typical examples of azo dye compounds which are drugs include phenazopyridine, which is a urinary tract analgesic, and sulfasalazine for inflammatory bowel disease ( Figure 1).
Although they are synthetically feasible by simple standard diazotisation and coupling reactions, there is still more to be carried out particularly with regards to diversification of functional groups [11]. Following on this trajectory, we present herein our findings on the synthesis and in vitro antimicrobial and anthelminthic evaluation of naphtholic and phenolic azo dyes derived from the coupling of various primary aromatic amines with 2-naphthol and phenol. Scheme 1 represents a general synthetic pathway for the azo dyes with their proposed names. e eight azo compounds are (E)-4-(4-nitrophenyl) diazenyl) phenol. ese compounds are coded based on their reacting components and are shown in Table 1. e synthesised azo compounds were then investigated for their antimicrobial and anthelminthic activities.

Chemistry and Instrumentation.
All reagents and solvents used in this present study were obtained from BDH chemicals as analytical or technical grade. Reaction progress was monitored using thin layer chromatography, which was performed by employing precoated silica gel plate (Merck F 254 ) and visualised with UV light (254 nm and 357 nm) or iodine vapour where necessary. Melting point data of the synthesised compounds were obtained by using one end or open capillary tubes on a Gallenkamp melting point apparatus (England) and are uncorrected. Compounds were purified by recrystallisation from suitable solvents appended to synthetic data in Table 1. Infrared (IR) spectra were recorded using FTIR PerkinElmer in the range 400-4000 cm −1 . Ultraviolet-visible (UV-vis) spectra were measured on a PerkinElmer spectrophotometer at 200-800 nm in methanol.

Synthesis of the Azo Compounds.
e synthesis of naphtholic and phenolic azo compounds is presented in Schemes 1 and 2.
Into a round-bottom flask (50 mL) equipped with a magnetic stirring bar, a mixture of primary aromatic amine (10 mmol) and concentrated HCl (36% w/v) was stirred until a clear solution was obtained. is solution was cooled to 0°C-5°C, and a solution of sodium nitrite in water (10 ml) was then added dropwise maintaining the temperature below 5°C. e diazonium salt solution was covered and kept in the ice bath. Starch iodide paper was used to test for the formation of diazonium salt-spontaneous formation of blueblack coloration. e diazonium salt was used immediately for the coupling reaction.

2.2.1.
Step II: Coupling Procedure. Into a round-bottom flask (50 mL), the corresponding 2-naphthol or phenol (10 mmol) was dissolved in NaOH (10% w/v, 50 ml) and cooled to 0°C-5°C in an ice bath. is solution was then gradually added to the cooled diazonium salt solution, and the resulting mixture was stirred at 0°C-5°C for 1 h. e resulting crude precipitate was filtered, washed several times with cold water, and recrystallized from appropriate solvent to yield the final azo compound [12] (Table 1)

Step I: Preparation of the Standard 96-Well
Plates. An amount of agar medium was melted in a steam bath or in a microwave oven. It was placed in a water bath and kept at a temperature of 55°C-60°C to prevent it from solidifying. A stock concentration of 50 mg/ml was prepared initially using methanol in a polymerase chain reaction (PCR) half-skirted 96-well plate to give a wide concentration range. Eleven different concentrations of azo compounds and reference drugs (ciprofloxacin, cefuroxime, amoxicillin, and fluconazole) were prepared using serial dilution. Each compound (2 μL) and reference drug dilution was pipetted and transferred into their corresponding wells in a standard 96-well plate, and the melted agar (200 μL) was dispensed. e wellplates were then shaken for 10 s to mix thoroughly. After the agar medium was dispensed, the plates were left in a safety cabinet undisturbed to solidify [13].

Step II: Spotting and Incubation of Prepared Well
Plates. Bacterial suspension (2 μL; 1 × 10 6 cfu/ml) was pipetted with a micropipette on to each well of the 96-well plate (each microorganism per plate) and allowed it to be absorbed into the agar for 5 min. e plates were sealed with parafilm and wrapped with aluminium foil. e plates were incubated inverted at 37°C. e growth was observed after 24 hrs. e well containing the lowest concentration of a compound for which no growth is observed and recorded within the incubation period is determined as the MIC (minimum inhibitory concentration) of that compound against the organisms [13].

Step III: Minimum Inhibitory Concentration (MIC)
Determination. Different concentrations of the azo compounds were tested against a panel of Gram-positive bacteria (Staphylococcus aureus, Enterococcus faecalis, and Streptococcus pyogenes), Gram-negative bacteria (Salmonella typhi, Escherichia coli, and Pseudomonas aeruginosa), and a fungus (Candida albicans) to determine their minimum inhibition concentrations (MICs) using the high-throughput spotculture growth inhibition assay (HT-SPOTi).
In the HT-SPOTi assay, molten agar maintained at 55°C-60°C was dispensed into 96-well plates to which 2 μL of serially diluted azo compounds have been added starting from a stock of 50 mg/mL. Bacterial suspension (2 μL; 1 × 10 6 cfu/ml) was added to each plate sealed and incubated for 18-24 h. e lowest concentration at which bacterial growth was completely inhibited by the compound was observed visually, and the MIC was recorded. e reference drugs used were ciprofloxacin, cefuroxime, amoxicillin, and fluconazole [13].

Reagents and Reference Antibiotics.
e reagents used included methanol (BDH Chemicals), dimethyl sulphoxide (DMSO) (BDH Chemicals) (ACS grade ≥ 99%), and piperazine citrate (Sigma Aldrich). e procedure for screening for the anthelminthic activity was with reference to Raghavendra and Kumar [14]. e anthelminthic activity was carried out on adult Ghanaian worms, Hyperiodrilus spp. e worms were collected from a local moist habitat and prior assay. ey were washed with water to remove dirt, soil, and faecal matter from the skin of the worm. ey were divided into groups in three Petri dishes with each containing three adult worms. Dimethyl sulphoxide (DMSO, 0.5% v/v) was used as the control, while piperazine citrate was used as a reference standard for this assay. Concentrations of 0.625, 1.25, 2.5, and 5 mg/ml were prepared for each of the azo compounds and the standard drug solutions. ree adult worms (triplicate determination) were then introduced into the Petri dishes containing solutions of the azo compounds and the standard drug. e worms in the Petri dishes were observed, and time taken for paralysis and death of worms were recorded. Paralysis was said to occur when the worms do not recover in DMSO (0.5% v/v). e worms were declared dead if there was no movement when shaken vigorously in warm water (50°C) associated with change in body color (fading to pale). e results are expressed as mean ± SEM (standard error of mean). Statistical differences were carried out using the analysis of variance and were considered significant when p < 0.05 (Table 2). Coupled product Scheme 1: General reaction for the synthesis of the naphtholic azo compounds [12].
All the eight compounds exhibited antimicrobial activities but to various degree on the six human pathogenic microorganisms. Considering the compound A2 for   instance, the chloro group at the para position of the compound A2 was not essential for activity; the substitution of the chlorine group increases lipophilicity, which could have made the molecule get stacked in biological membrane and cause nonbonding interaction with protein groups at the binding site [15]. e esterification of the carboxylic functional in the A1 group to the compound A3 (ester) diminishes activity especially against Salmonella typhi and Enterococcus faecalis; however, the activity of the ester was greatly improved against Escherichia coli by at least eightfold. e compound A1 had the greatest activity against Salmonella typhi and Streptococcus pyogenes with an MIC of 62.5 μg/mL followed by good activity against Staphylococcus aureus. All the compounds tested showed no activity against E. faecalis with the exception of the compound A1, which had little activity with an MIC of 500 μg/mL. e compound A4 (naphthol template maintained with −SO 2 NH 2 : sulphonamide mimic) showed increase in activity against E. coli and Candida albicans by at least fifteen-fold and onefold, respectively. e presence of the -SO 2 NH 2 group rather decreased drastically the activity of A4 against the other organisms. Considering the substituents on the compound A1 (carboxylic acid) and A3 (the ester group) (clogP: 5.24 (ChemBioDraw Ultra, Version 14)) are less polar than the carboxylic acid group, which allows it to penetrate lipid membranes. e ester group also makes the molecule behave as a prodrug of which is susceptible to microbial esterases to yield the active carboxylic acid group [16]. Notwithstanding this, the esterases released by the microbes could have degraded the ester hence decreasing the activity, or the ester remains inactive until it is acted upon by the microbial enzymes.
e compound A4 with the sulphonamide group exhibited an activity against Staphylococcus aureus with an MIC of 250 μg/mL in the assay. is means that the sulphonamide derivative is more active against Gram-negative E. coli. It has been noticed that antimicrobial activities of sulfonamides increases when bulky heterocycles are introduced on their structural templates. For instance, sulfasalazine and sulphamethoxazole are employed in treating various bacterial infections [17]. Overall, the compound A1 was the most active against the organisms, whilst A2 did not show any antimicrobial effect.
With the phenolic azo dye derivatives, the compound B4 showed moderate activity against Salmonella typhi with an MIC of 125 μg/mL and significant activity against E. coli and Staphylococcus aureus with MICs of 62.5 μg/mL, respectively. Compounds B1 and B4 showed moderate activity against Candida albicans with an MIC of 125 μg/mL. ere was drastic reduction in the activity of the compound B3 indicating that the presence of chlorine at the ortho position affects activity. e compound B1 with the carboxylic acid group had reduced the activity throughout against the microorganisms. Esterification of carboxylic acid with the ethyl group diminished the antimicrobial activity completely. Comparing A1 and B1 built on carboxylic acid template, the naphtholic derivative (A1) was more active generally against the organisms compared to its corresponding phenolic derivative.
Overall, B4 showed good antimicrobial activity against most of the organisms with significant MICs. e compound A1 is a broad spectrum; A4 is potent mainly against Gramnegative bacteria with very little activity against Grampositive bacteria; B4 is also a broad spectrum with little antifungal activity and hence holds potential for future development into novel antimicrobial agents. It can be concluded that the naphtholic derivatives are more potent than the phenolic derivatives in terms of the antimicrobial activity. Nevertheless, none of the synthesised compounds showed good activity against P. aeruginosa, which is a wellknown notorious Gram-negative organism due to its efflux pumps, resistant genes, and thick biofilm inhibition. Pseudomonas aeruginosa is now to be responsible for 65-80% of microbial infections, and hence, more work need to be carried out in structurally modifying this library of compounds to overcome Pseudomonas-related diseases [18,19].
For the anthelminthic activities, it was noticed that the time taken for paralysis and death to occur was increasing as the concentration of the azo compounds reduces. It is observed that compound B4 (phenol with the nitro group) had the best activity with low paralysis and death time. It can be observed that the phenolic derivatives of the azo compounds had the best activity. It could be said that the naphtholic derivatives have a bulky group as compared to the phenolic derivatives; this bulkiness could have prevented the compounds from reaching their target site, hence, undermining specificity. ere is the presence of steric hindrance in the naphtholic derivatives due to the position of the hydroxyl group, which could have affected the binding of the molecule. erefore, an extra benzene group was not necessary for paralysis of the worms but rather a simple phenolic moiety.

Data Availability
Data are available from the laboratory of Pharmaceutical Chemistry Department, Faculty of Pharmacy and Pharmaceutical Sciences, KNUST,, Kumasi upon request.

Conflicts of Interest
e authors declare that there are no conflicts of interest regarding the publication.