Study of Antibacterial Activity of Root Bark, Leaves, and Pericarp Extracts of Diploknema butyracea and Evaluation of Prospective Antioxidant Activity

This study was aimed to determine the antibacterial activity of root bark, leaves, and pericarp extract of Diploknema butyracea and to evaluate the prospective antioxidant activity, total flavonoid, polyphenol, and carbohydrate content. The plant parts were collected and extracted by cold maceration, using hexane, ethyl acetate, methanol, and distilled water. Phytochemical screening of different samples of D. butyracea in different solvents revealed the presence of varied extent of alkaloid, saponin, terpenoid, anthraquinones, tannin, cardiac glycoside, flavonoid, carbohydrate, polyphenol, protein and amino acid, resin, and phytosterol. Our study showed that methanolic root bark extract exhibited the potent antimicrobial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Klebsiella pneumonia with an average zone of inhibition of 17.33 mm, 14.33 mm, and 13.0 mm, respectively. Surprisingly, all of the extracts were insensitive to Escherichia coli. The lowest minimum bactericidal concentration (MBC), 4.6 mg/ml, was observed with the aqueous pericarp extract against S. epidermidis and the highest was of 50 mg/ml shown by ethyl acetate pericarp against K. pneumonia. Our results showed that both the polar and nonpolar components present in the different parts of D. butyracea exhibit prominent antibacterial activities against different bacterial strains. The in vitro 2,2′-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity showed that the methanol extract of root barks displayed the most potent antioxidant activity (IC50 : 6.1 µg/ml). The total polyphenol content of the plant part extracts was observed between 19.48 ± 0.23 and 123.48 ± 1.84 µg gallic acid equivalent/mg of dry extract weight. Likewise, flavonoid content ranged from 40.63 ± 1.28 µg to 889.72 ± 3.40 μg quercetin equivalent/mg of dry extract weight and total carbohydrate content ranged from 11.92 ± 0.60 µg to 174.72 ± 0.60 µg glucose equivalent per/mg dry extract weight. Overall, our study showed that the root bark, pericarp, and leaves extract of D. butyracea evinced prominent antibacterial properties against various pathogenic bacterial strains.


Introduction
Herbal-based traditional medicines have always been a part of human culture since the ancient time [1]. In the modern era, medicinal plants are considered as the center of attention for enormous investigation of their inherent biological effect [2].
Screening of natural plants, targeting specific therapeutic activity, has led to the revelation and discovery of clinically effective medicine to cope with life-threatening human disease [1][2][3][4]. In Ayurveda, the use of herbal extracts and nutritional supplements for the treatment of infectious diseases as an alternative or complementary medicine has been well documented and preserved for about 5,000 years. Allopathic medicines can undoubtedly cure a wide range of diseases. However, because of their unavailability, high prices, and unwanted adverse effects, many patients prefer to adopt the natural source of remedy [5]. In the current scenario, bacterial infectious diseases are a serious worldwide public health problem due to irrational use of antibiotics. As a result, diverse classes of multidrug resistant bacterial strains are being generated nowadays [6]. Increased rates of mortality and morbidity are due to the lack of long-term effective drugs and unaffordable cost of new generation antibiotics [7]. e problem of microbial resistance is growing and the prospect of the use of antimicrobial drugs is uncertain. is disastrous situation has compelled us to explore more successful antimicrobial agents using plant resources so that they will serve as an active therapeutic ingredient and lead molecules to the synthesis of optimized new drugs [8].
Diploknema butyracea, commonly known as the Indian butternut tree in English, is a medium-sized deciduous tree of about 20 m in height ( Figure 1) [9,10]. It belongs to family Sapotaceae and is widely distributed in the tropical and temperate regions, at an altitude of about 300-1500 m, primarily on hill slopes and cliffs [11], and is found in northern India, Tibet, Nepal, Bangladesh, Sri Lanka, and Bhutan [12]. It is popular with the name "Chyuri" in Nepal, "Indian-butter nut" in English, and "Chiura" or "Phulwara" in India [9,13]. e important ethnomedicinal assets of this plant are seeds, which are utilized for the production of butter or fat, known as Chyuri ghee and it has diverse uses including cooking and lighting lamps by the local communities [14]. Apart from that Chyuri seed butter has also been used to make cosmetic items, soaps, cosmetics, and other commercial products like cooking ghee and candles [9,11,15]. e ripen fruits are crushed and applied on topical areas for the treatment of skin ailments in animals as well as human beings. e bark juice of the plant is widely utilized to cure rheumatism, indigestion, asthma, ulcer, itching, allergy, diabetes, and tonsillitis [9,11]. e dried powder of stem bark is taken orally by mixing with water or milk to mitigate fever [11,16]. Dried powder of flower and petals is consumed as a tonic, for the soothing effect of the irritated throat and for increasing lactation. Flowers are an excellent source of honey production. e paste of fresh leaves is used to treat ulceration of the mouth and muscular pain [11,16,17]. e prime chemical constituents of Chyuri butter are triglycerides. Major fatty acids found in it are methyl ester form of saturated stearic acid (2.4%), saturated palmitic acid (66%), polyunsaturated oleic acid (2.6%), and monounsaturated linoleic acid (26%) [9]. Besides, different feeding deterrent saponins, MI-III and MI-I [18], and aromatic components such as methyl-2-furoate, heptane, 3,4dimethyl-1,2-cyclopentadiene, lauryl alcohol, and trans,trans-2,4-heptagonal are also present in the fruit [19]. Diverse pharmacological effects such as the antioxidant effect of fruit pulp [20], antifungal activity of seed extract [21], anti-inflammatory effect [22], and antibacterial activity [23] of stem bark extract along with feeding deterrent and insect growth inhibitory effect of seed extract [18] have been reported for this plant. However, there is no scientific claim on the antimicrobial and antioxidant effects of D. butyracea pericarp, root bark, and leaves till date. us, this study was aimed to determine the antibacterial activity of root bark, leaves, and pericarp extract of D. butyracea and to determine their antioxidant activity, total flavonoid, polyphenol, and carbohydrate content with phytochemical screening.

Plant Extracts Preparation.
Firstly, the collected leaves, root barks, and unripe fruits were washed with fresh distilled water. Unripe fruits of D. butyracea were first separated into flesh and seeds. Only flesh (pericarp) was chopped into small pieces and left for shade drying for 2 weeks. A similar procedure was adopted for the bark. However, leaves were directly left for shade drying without cutting. e naturally air-dried leaves and root bark were comminuted with a grinder to a fine powder and passed through the #40 mesh sieve (0.381 mm of pore size). Because of the sticky nature of the dried fruit slices, we performed their direct extraction.
In order to ensure the optimal extraction of the plant parts, we used triple cold maceration process. After single maceration with periodic manual shaking in every 6 h for 72 h, the menstruum was collected and marc was further extracted with the same amount of fresh solvents. e whole procedure was repeated three times. Briefly, 200 g each of leaves, root barks, and fruit pericarp of D. butyracea were macerated with 1,000 ml of hexane, ethyl acetate, methanol, and water. e liquids from each step of maceration were strained, filtered and pooled and dried at 40°C to obtain a gummy concentrate using rotatory evaporator, and the extracts were stored in refrigerator at 4 ± 1°C until use.

Extractive Yield Value.
e extractive yield of D. butyracea root bark, leaves, and pericarp in hexane, ethyl acetate, methanol, and water was calculated by using the following equation: Extractive yield

�
Weight of the extract obtained(g) Weight of crude drugs used for extraction(g) × 100%. (1)

Muller Hinton Agar (MHA) Media Preparation and
Subculture of Bacterial Strains. e antimicrobial activity was measured by the disc diffusion method. 38 g of MHA was suspended in 1000 ml distilled water in a conical flask. e media dissolved completely and sterilized in an autoclave at 121°C for 15 min at 15 lbs pressure. e hot conical flask media was allowed to cool to 40-50°C in sterilized laminar airflow. e media was poured into each Petri plate and dropped to set. Two hardened media were incubated at 37°C for 24 h to check the possible contamination, and the remaining was refrigerated at 5°C. For subculture, the inoculating loop was inflamed in a burner flame to transfer the bacteria sample to the agar plates. e inoculating loop was cooled and dipped inside the tube to pick up the microorganism. en, the loop was streaked across the surface of the agar plate in a zigzag pattern. In this manner, all the test organisms were subcultured in separate agar plates with proper labeling. e subcultured plates were incubated at 37°C for 24 h before inoculation. All the experiments were completed in aseptic condition with laminar airflow.

Preparation of Bacterial Suspension/Inoculum.
Initially, nutrition broth media was prepared and sterilized. After that, 5 ml nutrient broth was poured into four different sterilized test tubes. Bacterial suspensions of S. epidermidis, S. aureus, E. coli, and K. pneumonia were prepared to suspend bacteria (from subculture media) with the inoculating loop to each respective test tube and incubated at 37°C for 24 h. e turbidity of the inoculums suspension was compared with 0.5 McFarland solutions.

Screening and Measurement of Zone of Inhibition (ZOI).
A sterile cotton swab stick was dipped into the turbidity-adjusted bacterial suspension. After that, the dried surface of the media plate was inoculated by rubbing the cotton swab stick (loaded with microorganisms) over the entire sterile media surface. e same technique was repeated for each microorganism. Finally, media plates were divided into four equal parts to insert the standard antibiotic disc and filter disc, containing sample extracts, blank control, in equal distance. 10 µg/disc of Ciprofloxacin and Gentamycin were used for gram-negative gram-positive bacteria, respectively. To load the test sample, 10 µl of each extract (1 mg of extract per disc) were poured into two paper discs (doublet manner) and the third paper disc was used for negative control (10 µl DMSO). All the plates were incubated at 37°C for 24 h. All the measurements were examined in triplicate. After 24 h of incubation, the culture media was taken out from the incubator, and the inhibited areas (ZOI) by the different extract and antibiotics were measured in mm, with the help of digital Vernier Caliper.

Determination of MIC and MBC.
e twofold serial broth microdilution technique was adopted to calculate the MIC values of all the plant extract, against four different test organisms. A total of 10 vials were labeled and sterilized; then, 750 µl of sterilized Mueller-Hilton Broth (MHB) was transferred into each vial. For the sample solution preparation, 200 mg/ml of stock solution was prepared in DMSO, subjected to serial dilution, using a 1 : 1 mixture of DMSO and water to prepare sample solutions of 10 different concentrations (200 mg/ml-0.390625 mg/ml). After that 250 µl of sample solution was transferred into a corresponding vial containing 750 µl of MHB, so that the final concentration of sample ranged from 50 mg/ml to 0.09765 mg/ml. Bacteria with an inoculum of about 1 × 10 5 CFU/ml were loaded into each vial. For the preparation of microorganism inocula, broth culture was incubated for 12 h, and turbidity of the suspension was adjusted to the turbidity of 0.5 McFarland standards. One inoculated vial was used as a negative control, to ensure broth suitability for growth of microorganisms. Also, 4% DMSO was tested as a blank control. After the incubation of the sample containing broth media, for 24 h at 37°C, the MIC value was determined. MIC was taken as the lowest concentration that prevented the visible growth of the bacterial culture. e easy technique to observe the inhibition of growth is the absence of turbidity in the examined tubes. But, it was very challenging to ensure whether the turbidity was due to the nature of plant extract or due to the growth of the bacteria.
us, MBC was investigated to determine the minimum concentration of the plant extract that can completely kill the tested microorganisms.
For the MBC determination, the refrigerated MHA Petri plates were incubated at 37°C for 45 min and transferred into the sterilized laminar airflow (LAF) hood. After that, samples from each diluted test tubes (obtained after MIC examination) were subcultured on MHA plates followed by incubation for the next 24 h at 37°C. Finally, the minimum concentration of plant extract that completely prohibited the microorganism growth over media surface was noted as the MBC.

Antioxidant Activity Determination.
e antioxidant activity of plant extract was checked by using DPPH (1,1diphenyl-2-picrylhydrazyl) free radical scavenging activity, according to previous methods with slight modification [29][30][31]. At first, the stock solution of 0.1 mM of DPPH, 1 mg/ml of ascorbic acid, and test solutions were prepared in ethanol. Ascorbic acid solution thus prepared was diluted into different concentrations (10 µg/ml, 5 µg/ml, 2.5 µg/ml, and 1 µg/ml). For the DPPH assay, 4 ml of different extract solutions (31.25 μg/ml, 62.5 μg/ml, 125 μg/ml, 250 μg/ml, 500 μg/ml, and 1000 μg/ml) of the sample was mixed with 4 ml of DPPH solution (0.1 mM) and incubated in dark place. After 30 min, the absorbance of the sample mixture was monitored at 517 nm, with the help of a UV spectrophotometer. Methanol and ascorbic acid were chosen as negative and positive controls, respectively. All the measurements were examined in triplicate. e free radical inhibition percentage was determined calculated using the following formula: where A 0 is the absorbance of DPPH solution and A 1 is the absorbance of the sample.

Determination of Total Phenolic Content, Total Flavonoid
Content, and Total Carbohydrate Content. Total phenolic content was determined using the Folin-Ciocalteu (FC) method with a trivial modification of previous research, using gallic acid as a standard. In the study, different concentrations of gallic acid were prepared. e extract solution of 1 mg/ml concentration was made from the ethanolic stock solution. 1 ml of ethanolic stock solution was treated with 1 ml (2N) FC reagent followed by 5 ml distilled water and was shaken for 5 min. Subsequently, 1 ml of 10% Na 2 CO 3 was added and incubated for 1 h at room temperature. e absorbance was measured utilizing a UV Spectrophotometer at 765 nm against a blank (without extract). All the measurements were evaluated in triplicate [29]. e total flavonoid content was determined using the method used in similar research study. A standard flavonoid compound was quercetin. Different concentrations of quercetin were prepared from the stock solution (1 mg/ml) using ethanol as a solvent. 1 mg/ml concentrations of the pericarp, leaf, and root bark extract were prepared. 1 ml of plant extract was dissolved in 4 ml of distilled water and 0.3 ml of 5% NaNO 2 . After 5 min, 0.3 ml of 10% AlCl 3 was added and incubated for 5 min. en, 2 ml of 1M NaOH was added to the solution. Similarly, a blank solution was prepared without a sample. All the reaction mixtures were incubated for 30 min at room temperature, followed by the absorbance measurement at 415 nm, against the blank. All the measurements were examined in triplicate [29].
Total carbohydrate content in different extracts of D. butyracea was determined by the phenol-sulphuric acid method, adapted by the previous study. In this test, the standard compound was glucose. Firstly, 1 mg/ml of the stock solution was prepared. e different concentrations of glucose standards (15.625 μg/ml, 31.25 μg/ml, 62.5 μg/ml, 125 μg/ml, 250 μg/ml, and 500 μg/ml) were prepared by serial dilution technique. In 10 ml of the test tube, 2 ml of the sample (1 mg/ml), 1 ml of the 5% phenol solution, and 5 ml of the concentrated sulphuric acid were mixed properly and kept for 10 min. en, the tube contents were mixed and placed in a water bath at 25-30°C for 20 min. e absorbance readings of the blank and the samples were measured at 490 nm. All the measurements were examined in triplicate [29].

Statistical Analysis.
All the experiments were performed three times and the data were presented as mean ± SD. Statistical significance of differences was calculated by one-way ANOVA and Tukey's test.

Extractive Yield Value.
e extractive yields of D. butyracea root bark, leaves, and pericarp in hexane, ethyl acetate, methanol, and water extract are shown in Table 1.

Phytochemical Screening.
A qualitative examination of phytochemical is a key footstep to acquire the scientific information about the presence of medicinally useful secondary metabolites in the plants, revealing a crucial role towards the beneficial medicinal and physiological activities such as antiviral, antimicrobial, anticancer, antioxidant, antidiabetic, and antimicrobial activities [23]. Phytochemical screening of D. butyracea, in different solvents, revealed the varied extent of alkaloid, saponin, terpenoid, anthraquinones, tannin, cardiac glycoside, flavonoid, carbohydrate, polyphenol, protein and amino acid, resin, and phytosterol presence. In our study, all the extracts were tannin-free. Protein and amino acid, and anthraquinone were absent in leaf and pericarp extract. Similar results were recorded in other studies [13,20]. e results are summarized in Table 2.

Antibacterial Test.
A total of 12 different extracts, obtained from the leaves, root bark, and pericarp of D. butyracea, were screened for their antibacterial activity against four different bacterial strains. eir antibacterial potency was quantitatively confirmed by an inhibition zone absence or presence all over the disc, loaded with the extract. e result confirmed that extracts are more sensitive to gram-positive bacteria in comparison to gram-negative (Table 3). Generally, plant extracts are more active against gram-positive bacteria than gram-negative bacteria due to lipopolysaccharide composition in the multilayered cell wall of gram-negative strains [32,33]. In this study, methanolic bark extract was reported to be the most significant against S. aureus (ZOI-17.33 mm), S. epidermidis (14.33 mm), and K. pneumonia (13.00 mm). However, the extract remains insensitive against E. coli. Also, only pericarp ethyl acetate extract was reported to be sensitive against both gramnegative strains. e ethyl acetate leaves and aqueous leaves extract flaunted antibacterial activity among the leaves, against K. pneumoniae. Between the two gram-positive bacteria, S. aureus was more sensitive than S. epidermidis. In the case of gram-negative bacteria, plant extracts were more effective against K. pneumonia than E. coli. Figure 2 depicts the ZOI produced by methanolic bark extract against two gram-positive strains.
Total 17 samples showed measurable ZOI, which were further screened for MIC and MBC. However, MIC could not be quantified because of the uncertainty of whether turbidity was due to the bacteria growth or due to the plant extract. us, MBC was calculated and expressed as mg/ml. e MBC values of different investigated samples were in the range from 4.16 mg/ml to 50 mg/ml. e maximum MBC value of 50 mg/ml was exhibited by ethyl acetate pericarp extract against K. pneumonia and the minimum, i.e., 4.16 mg/ml, by aqueous pericarp extract against S. epidermidis. e methanolic bark was able to kill both gram-positive strains as well as gram-negative strain K. pneumonia at the same concentration, i.e., 25 mg/ml. However, only ethyl acetate extract of pericarp could kill E. coli (12.5 mg/ml). All the results are depicted in Table 4. Similarly, Figure 3 [18]. Also, various antibacterial triterpenoids such as the presence of chemical constituents like triterpenoids (α-amyrin acetate, β-amyrin acetate, and friedelin) were reported from the bark of D. butyracea [34]. ese compounds might be responsible for the antibacterial effect. However, bioassay-guided fractionated isolation is necessary to identify the antibacterial compounds present in this plant.

Antioxidant Potency Determination by DPPH Radical Scavenging Activity.
e hydrogen atom or electron donation ability of each plant extract against DPPH free radical was measured from the bleaching of violet-colored ethanol solution of DPPH. e DPPH radical absorbs UV radiations at 517 nm. e radical scavenging activity was determined by monitoring the decrease in absorbance [22,29]. Among three individual parts, our investigation flaunted that D. butyracea methanolic root bark extract exhibited the highest capacity to reduce the DPPH free radical (90.52 ± 0.13%) even at the concentration of 200 µg/ml and the lowest scavenging capacity was exhibited by hexane pericarp extract (18.18 ± 0.2% at 1000 µg/ml). Interestingly, the IC 50 value of methanolic root bark (6.1 µg/ml) was reported to be almost similar to that of standard ascorbic acid (5.15 µg/ml).
e IC 50 value of the D. butyracea aqueous stem bark was determined to be 8.43 µg/ml in previous research [22]. In a former study, IC 50 of the methanolic pericarp (104 µg/ml) [20] was found almost similar to this study (111.3 µg/ml). Among different solvents, the most significant scavenging effect was exhibited by methanolic extract in all the plant parts. On the top, in our study, extract having higher phenolic and flavonoid contents had higher radical scavenging affinity, proportionally. No significant scientific studies have been conducted yet, regarding the antioxidant activity of D. butyracea root bark and leaves. e percentage of free radicals scavenged by ascorbic acid at different concentrations is represented in Table 5, whereas  Table 6 shows the free radicals scavenged by methanolic bark at diluted concentrations. Figure 4 represents the IC 50 values Alkaloid Resin Protein and amino acid ++      us there is a strong correlation between antioxidant potency and the total polyphenol content of many plant species. It has been proven that phenolic compounds are efficient hydrogen donors and serve as a very good antioxidant [35]. In our study, the quantitative estimation of total phenol was accomplished by using Folin-Ciocalteu reagent and the data were expressed as gallic acid equivalent (GAE)/mg of dry extract. Table 7 shows total phenol content expressed as µg gallic acid equivalent per milligram dry extract weight.
ere is variation in total phenol content ranging from pericarp hexane extract (18.7 ± 0.23 μg GAE/mg dry extract weight) to methanolic root bark extract (222.16 ± 1.33 μg GAE/mg dry extract weight). From the data of Table 7, it is observed that extraction solvent has a great effect on the phenolic content of the different parts. Also, there is great variation among different plant parts in the same solvent.
e statistical analysis showed a significant difference (p < 0.05) in the total phenolic content: when each part was compared in different solvents as well as when different parts were compared in the same/each solvent. It is to be noted that the significantly highest phenolic content was recorded in root bark extract whereas the significantly lowest amount was recorded in the hexane extract of the leaf. Also, methanol was found to be the best solvent to extract phenolic compounds significantly in all the investigated parts of the D. butyracea plant. Furthermore, the phenolic content of aqueous stem bark determined in a similar study (228.53 µg GAE/mg) [22] was reported to be very high in comparison to the aqueous root bark of our study (62.16 µg GAE/mg). In another study, the total phenolic content of hydromethanolic extract of the pericarp (40.4 µg GAE/mg) [36] was less than methanolic   Flavonoids are a highly diversified and widespread group of natural phenolic compounds. Hydroxyl position present in the flavonoid compounds governs antioxidant properties, and it depends on the electron or hydrogen donation capacity of flavonoid to a free radical [36]. In our study, quantitative determination of total flavonoid was performed by precipitating with aluminum chloride (AlCl 3 ) in an alkalinized medium. Results for the total flavonoid content are depicted in Table 7. Among the studied D. butyracea samples, there is variation in total flavonoid content ranging from hexane pericarp extract (40.63 ± 1.28 μg QE/mg dry extract weight) to methanolic bark extract 889.72 ± 3.40 μg QE/mg dry extract weight. It is obvious from Table 7 that the extracting solvent has a significant effect on the flavonoid content of the different parts and also each part has different content even in the same solvent. e statistical analysis showed a significant difference (p < 0.05) in the total flavonoid content when each part was compared in different solvents as well as when different parts were compared in the same/each solvent. In this study, the order for the flavonoid content in different samples of D. butyracea is as follows: root bark > pericarp > leaves. Among the leaves extracts, the highest flavonoid content was found in hexane extract 297.90 ± 0.74 µg QE/mg. Similarly, the highest flavonoid content among the pericarp extract was shown by the ethyl acetate pericarp 649.72 ± 5.60 µg QE/mg. e flavonoid content of all the samples was documented for the first time. Although isolation of flavonoids compounds from the D. butyracea leaves, root bark, and the pericarp is not reported yet, quercetin and dihydroquercetin were isolated from the nutshell [37].
Carbohydrates are the abundant organic molecule produced during photosynthetic activity and major structural component of a plant cell. Carbohydrates are the vital energy source that regulates the metabolic processes, stimulates insulin secretion, acts as a powerful neurotransmitter, and alters serotonin concentration [38]. e quantitative determination of total carbohydrate content was carried out using phenol-sulphuric method in terms of glucose equivalent. Table 7 shows total carbohydrate content expressed as µg glucose equivalent per milligram dry extract weight.
ere is variation in total carbohydrate content ranging from pericarp hexane extract (11.92 ± 0.60 µg glucose/mg dry extract weight) to methanolic bark extract (174.72 ± 0.60 µg glucose/mg dry extract weight). e result showed that the extracting solvent has a significant effect on the carbohydrates content of the different parts and each part has different content although extracted in the same solvent. e statistical analysis showed a significant difference in the total carbohydrates content when each part was compared in different solvents as well as when different parts were compared in the same/each solvent as mentioned in Table 7. As shown in Table 7, a moderate amount of carbohydrate was detected in the entire sample. Also, carbohydrates got undetected in hexane leaf extract of D. butyracea. e methanolic extract of root bark contained significantly the highest amount of carbohydrate among the parts and solvents whereas hexane extract of pericarp has the lowest amount detected.
Notably, our study shows the higher total flavonoid content than the total phenolic content in most of our samples. is observation, however anamulous, is consistent with the similar results from previous studies [39][40][41]. Our speculation for this anomolous result is that such methods for the specific tests are completely different; the standard used in these two tests is different (we have used quercetin for flavonoid test whereas gallic acid was used for phenolic content test); both methods used for flavonoid and phenol test are not the absolute quantitative measurement, rather they give relative determination in terms of gallic acid and quercetin equivalent, influence of the chemical nature of the flavonoids (such as tannin types of flavonoids) and phenol compounds (such as compounds having less −OH groups on the ring); and total phenolics assay may not detect all the phenolics (as this can depend on the composition phenolic compound) [42,43]. ese might be the possible reasons for higher flavonoid content.

Conclusion
e present study shows that methanolic extract of D. butyracea root bark possesses potent antioxidant and antibacterial activity. It may be due to the polyphenol and flavonoid components.
is study highlights that the leaves, bark, pericarp extracts of D. butyracea in methanol can be strongly recommended for different biological properties. e study dispenses a prime basis to draw on the extract in the therapeutics of variant maladies. e methanolic extract resonated with the potent antioxidant activity. e root bark, pericarp, and leaves extract of D. butyracea revealed evinced prominent antibacterial properties against various pathogenic bacterial strains, recommending the significant utilization in the mitigation of diverse microbial diseases like diarrhea, urinary tract infection, skin infection, dysentery, dental problems, etc. However, further extensive research with great emphasis on the clinical model and the mechanism of action of antibacterial effect is needed to justify ethnomedicinal use of this plant and to pursue the scientific journey of plantbased antimicrobial drug development for safe and effective health care service.

Data Availability
All the data used to support the result of this research are available from Jitendra Pandey and Pramod Aryal upon request.

Disclosure
is research was performed as part of the partial fulfillment of an academic degree at Crimson College of Technology affiliated with Pokhara University.