Microstructural Characters of Lyctinae and Dinoderinae ( Coleoptera : Bostrichidae )

Thirty-three species belonging to the bostrichid subfamilies Lyctinae and Dinoderinae were examined by low vacuum ESEM without sputtering. Eight types of microsculpture, five types of hair insertion, and 14 types of hair were found on the elytral disc and declivity. The different types of microstructure are described and illustrated with ESEM photos of each type. Surface microstructures provide additional taxonomically useful characters which can help to distinguish easily confused species. The study also showed that specimens can be examined under ESEM without any damage.


Introduction
The quality and number of characters is often a limiting factor in phylogenetic investigations.Thus, searching for new sets of characters is an important task in taxonomy.It is influenced both by detailed knowledge of the animals under investigation and by new techniques.In this study, the Environmental Scanning Electronic Microscope (ESEM) has been used to investigate microstructural characters of the cuticular surface of bostrichid beetles.Characters, such as hair, punctures, and tubercles, which are of significant value both to practical taxonomic work, and to further phylogenetic investigations of the Bostrichidae are illustrated.
The family Bostrichidae has a world-wide distribution but is mainly found in tropical and arid areas.There are more than 550 known species which vary from small to very large in size.The classification used in this paper is that of the recent catalogue of the family [1].Bostrichidae mainly feed on and breed in bamboo, timber, rattan, stored grain, and products made from bamboo and timber.Their recorded plant hosts extend to at least 30 families [2], and probably almost any family with woody species can be attacked.In this paper, species in two of the subfamilies of Bostrichidae: Lyctinae and Dinoderinae are considered.A few species of lyctines have become important pests of timber, wooden objects and ancient structures [3][4][5].Some species of Dinoderinae are important pests of bamboo and stored grain [3,4,6].
On average, Lyctinae have the smallest body size of all bostrichids.Because of their small size, lyctines, as well as the taxonomically difficult genus Dinoderus (Dinoderinae) [7] are quite difficult subjects for classical investigation by light microscopy and deserve detailed morphological investigation with ESEM.Other genera of Dinoderinae are not as difficult to identify as Dinoderus, but to provide a more comprehensive concept of the microstructural characters of the subfamily Dinoderinae, some dinoderines in other genera have been examined.
A report on the use of microstructures to separate four bostrichid species in the genera Minthea and Dinoderus was presented by Liu et al. [8].The present paper expands that study to include a greater range of genera and species.

Materials and Methods
In order to avoid any damage to the specimens, an FEI (model: Inspect-S) Environmental Scanning Electron Microscope (ESEM) was used to examine the elytral cuticle of specimens.Specimens were not removed from their mounts or pins, nor was the surface of the specimens coated with gold or other metals (i.e., the specimens were investigated Psyche unsputtered).The settings of the ESEM were beam spot from 2.5 nm to 4.0 nm, acceleration voltage between 15 kV and 30 kV, and low vacuum between 0.6 mbar and 0.8 mbar.Particular attention was paid to the middle part of the elytral disc.
Thirty-three species were examined as listed below.Specimens were loaned from the Natural History Museum, London or form part of the private collection of the author.

Results
The lyctines do not have a distinct elytral declivity, but dinoderines do.Usually the patterns of punctures, tubercles and hairs on the elytral disc are different from those on the declivity, so the morphological patterns of microstructures on the elytral disc and on the declivity will be discussed separately for the Dinoderinae.
There are two basically different types of punctures in Lyctinae, hair-bearing and nonhair-bearing punctures.Hairbearing punctures always correspond in diameter to the root of the hair.Nonhair bearing punctures vary in size, depth and shape of the rim.Hence only nonhair bearing punctures of Lyctinae are discussed here.
Eight types of microsculpture, five types of hair insertion and 14 types of hairs were found on the elytral disc and declivity of the 33 species investigated.Short descriptions of the different types of microstructures observed are listed below.Each description corresponds to an ESEM photo (Figures 1, 2 and 3).In the captions to the figures, structures photographed on the elytral declivity are noted; all other photos are from the elytral disc.Type n.Lamelliform hair (Figure 3(l), arrow).

Types of microsculpture:
The distributions of the different types of microsculptures and hairs on the elytral disc and declivity vary greatly in the different species of lyctines and dinoderines.To give an overview, the distributions of the different types are presented in Tables 1 and 2.
In the subfamily Lyctinae, there are two types of punctures.The large shallow or deep one has no hair inside (microsculpture types a-d), the small one is hair-bearing.Minthea usually has two types of interstrial hairs on the elytra and a stria separates the two rows of different types of hairs (Figures 3(d) and 3(l)).The large erect hairs (type m) are usually far more obvious than the fine and soft hairs on alternate interstriae (types k and n).Only in M. bivestita, the second type of hair is as large as the major one.In this species the hairs are thicker and have 4 serrulate longitudinal ridges (type i).
The morphology of the hairs on Cephalotoma is very different from other species.There are two types of hairs on Cephalotoma, one is short, the other one is long, and both are strongly flattened upward from the insertion of the hair (Figures 3(d  of the puncture (hair insertion type c).Prostephanus has long simple hairs (hair type a) inserted on the rim of subcircular punctures (hair insertion type c).However, Stephanopachys has a tubercle next to each puncture (microsculpture type g and h) and a simple hair on the top or side of the tubercle (hair type a and k, and insertion of hair type d and e).This suggests that the tubercle may have evolved from the prominence of the rim of the puncture.
With regard to the way in which the hairs are inserted, type a (away from the microsculpture) is found throughout the lyctines except for Cephalotoma.In C. perdepressa, hair insertion type c (inside puncture and close to the rim) is found and this is also present in Prostephanus and Dinoderus.In Rhyzopertha dominica, the insertion of the hairs is next to a puncture (type b).It is on the side of a tubercle (type e) in Stephanopachys quadricolllis and on the top of a tubercle (type d) in S. rugosus.S. substriatus is the only species with a different insertion of its hair on elytral disc and declivity, next to a puncture (type b) on disc and on the side of a tubercle (type e) on declivity.

Discussion
Lesne [9,10] and Gerberg [11] both examined the elytral sculpture of bostrichids, and used it to provide diagnostic characters for species.However, they did not examine the sculpture by ESEM, so they only found the larger microsculptural characters.Santoro [12] examined 31 species of Lyctinae under the light microscope by making the elytra transparent.He stated that the characters of punctures and hairs could be used to determine most of them at species level.However, his method requires the removal of the elytra and their treatment with potassium hydroxide before mounting, and is not suitable for rare or type specimens.
ESEM facilitates the examination of the microstructures on specimens.Not only the microsculpture, but also the shape of hairs, and the details of their insertion can be observed well under ESEM without any damage to the specimens.It is evident that microstructural characters can help to distinguish easily confused species and should be considered in future investigations.

Microsculptures on the Elytra of Lyctinae.
In the subfamily Lyctinae, the nonhair bearing punctures are usually large and shallow apart from Minthea squamigera, which is the only species with very deep pits in the punctures (type d).Santoro [12] found there are two types of punctures on the elytra of M. squamigera, "single" and "double" in which two punctures seem to have fused.The ESEM picture of M. squamigera shows the two types of punctures (Figure 1(d)).The double punctures occur only in M. squamigera, and not in other species of Minthea (cf.also [12]).To sum up, M. squamigera has three types of punctures: hair-bearing small punctures, nonhair bearing single deep punctures and nonhair bearing double deep punctures.
The shallow punctures shown on the ESEM pictures usually correspond to those shown with a blurred rim in Santoro's [12] pictures.M. squamigera has the deepest punctures (type d) in Lyctinae according to this study, and shows the most distinct rim in Santoro's photographs.Santoro [12] suspected the structure under the punctures to be different in the shallow and deep ones.It would be interesting to study this further using a Scanning Transmission Electron Microscope (STEM).The complete scientific name of each species is given in the list of material.

Hairs on the Elytra of Lyctinae.
In Lyctinae, usually only one type of hair is found on the elytral disc, but Minthea and Cephalotoma both have two types of hairs on the elytral disc.The small, slender hairs in particular have been overlooked by most previous authors.Gerberg [11] mentioned the rows of the small hair only when he described Minthea obsita and M. rugicollis, but not when describing M. reticulata and M. squamigera.So his description of the rows of hair-each row of erect hair separated by two rows of large, circular, and shallow punctures-was somewhat misleading.
The descriptions of the hair on the elytra of Minthea differ among previous authors.Kraus [13] described the hair as "erect bristles", Lesne [14] described them as "erect claviform setae", and Gerberg [11] described them as "erect, flattened, whitish hair".Santoro [12] classified Minthea into the group of Lyctinae with lanceolate or claviform hair.The type species, M. squamigera is even named for its scale-like hairs.The morphology of hairs on the elytra of Minthea becomes very clear under ESEM.The erect hair is not flattened at all.The major type of erect hair on the elytra of Minthea is the seta with an open brush-like end and 4-6 serrulate longitudinal ribs (type m).Hence, the shape of the major hair is similar to the stout claviform hair with a densely divided tip, not scale-like or lanceolate.
Only one species of Cephalotoma has been examined in this study.There are three species in the genus Cephalotoma and four species in the closely related genus, Lyctoderma.Even though the morphology of the hairs on the elytral disc of C. perdepressa distinguishes it from other lyctines very well, further studies are needed to clarify the morphology of the hairs in both genera.This will certainly help to evaluate the phylogenetic relationships between these two genera and other lyctines.

Microstructural Characters on the Elytra of Dinoderinae.
The type of puncture differs between the elytral disc and declivity in Dinoderus brevis and D. minutus.There are shallow subcircular punctures with a corrugated rim (type e) on the disc, and shallow subcircular punctures with corrugated rim and convex inside (type f) on the declivity.Other Dinoderus spp.all have the type e puncture on both elytral disc and declivity.The difference in the punctures was not discussed in Lesne's [15] paper, but is briefly mentioned in Fisher's [16] revision.There are usually two different types of hair on the elytral disc and a third type of hairs on the declivity in Dinoderus (see Table 2).No previous author mentioned the difference in the types of hair on the disc and on the declivity.
Fisher [16] described the hairs of Dinoderus as "short, erect, rather stiff, yellowish hair", Lesne [15] simply described the hairs of D. distinctus as "very short".Of the two types of hair usually found on the elytral disc of Dinoderus, one type consists of fine simple hair (types a and j) as mentioned by Fisher [16], the other has a densely divided forklike end and/or is serrulate on one side (types f, g, and k), and is usually longer and of greater diameter.The latter were not found by Fisher [16], nor by Lesne [15].Some Dinoderus species have no fine simple hair on elytral disc, but nevertheless have two different types of hair of types f, g, or k.
Except for Stephanopachys, all dinoderines have only hair-bearing punctures in which the hair is inserted inside Psyche the puncture close to the rim.This character distinguishes Dinoderinae from Lyctinae.

Conclusion
The special advantages of the scanning electronic microscope are the possibility to depict three dimensional structures with a very large depth of focus, and the fact that the image is built up almost purely from the surface of the specimen (e.g., [17]).The apparently three-dimensional picture is very suitable to describe fine structures.For example, the broad hair of Minthea have often been described as scale-like hair before we examined them by ESEM.However, the ESEM showed the hair is not flat and scale-like at all, but a thick, ridged hair with an open brush-like end.
Usually biological specimens are sputtered with gold to produce a conducting coating on the surface to avoid charging, which is a well known problem to all those working with biological specimens using electron microscopy.It is usually not possible to sputter rare specimens or types, but the ESEM can be used to examine such specimens without coating them.The ESEM uses a low vacuum in the specimen chamber even though the acceleration voltage is not low.It produces clear images of the microstructures of the uncoated specimens.This study not only found additional taxonomically useful characters in the microstructures on the surface of specimens, but also showed that we can now examine specimens without any damage under ESEM.
) and 3(e)).Hence, the morphology of the hairs clearly distinguishes Cephalotoma from other lyctines and dinoderines.Figures3(d) and 3(e) show both lateral and dorsal views of these special flat hairs.In Dinoderus, usually the hair is inserted inside the puncture and close to the rim, or on a prominence on the

Table 1 :
Distribution of the different types of microsculpture ( * : appear on elytra, D: appear on decilivity).

Table 2 :
Distribution of the different types of hairs ( * : appear on elytra, D: appear on decilivity).