Research Article Coexistence and Competition between Tomicus yunnanensis and

Competition and cooperation between bark beetles, Tomicus yunnanensis Kirkendall and Faccoli and Tomicus minor (Hartig) (Coleoptera: Scolytinae) were examined when they coexisted together in living Yunnan pine trees (Pinus yunnanensis Franchet) in Yunnan province in Southwest China. T. yunnanensis bark beetles were observed to initiate dispersal from pine shoots to trunks in November, while the majority of T. minor begins to transfer in December. T. yunnanensis mainly attacks the top and middle parts of the trunk, whereas T. minor mainly resides in the lower and middle parts of the trunk. The patterns of attack densities of these two species were similar, but with T. yunnanensis colonizing the upper section of the trunk and T. minor the lower trunk. The highest attack density of T. Yunnanensis was 297 egg galleries/m2, and the highest attack density of T. minor was 305 egg galleries/m2. Although there was significant overlap for the same bark areas, the two species generally colonize different areas of the tree, which reduces the intensity of competition for the relatively thin layer of phloem-cambium tissues where the beetles feed and reside.

T. yunnanensis is frequently joined by T. minor (Hartig) in attack of Yunnan pine trees in the southwest of China, Yunnan province [2,7].In most of the cases, the two species live in the same area and feed on the same host trees [5,7].Several studies have reported that there is a general competition between T. minor and T. yunnanensis [2,5,[7][8][9][10].However, the specific mechanisms and the extent of the competition are unclear.An investigation on the cooperation and competition between these two bark beetle species to obtain food may indicate new approaches of integrated control of pest bark beetles, especially T. yunnanensis and T. minor which are very difficult to control and monitor [11][12][13][14].Thus, the goals of the present studies were to investigate the changes in distribution on the host tree of the beetles during the autumn dispersal from shoots to trunks and to determine the mutual influence of the two Tomicus species on each other's attack distribution and reproduction within the tree.Additional knowledge about the two pest bark beetle species' attack distributions and densities, colonization sequences, migratory movements, and competitive interactions may provide insights into more efficient management practices.Every shoot was carefully examined, and any bark beetles that were found were collected and distinguished by species with a binocular microscope.In addition, one to two pine trunks that had been colonized by T. yunnanensis or T. minor were selected and cut down every week, or approximately 17 trees.The tree trunks were divided into 0.5 m long logs, and each log was carefully peeled of bark to reveal egg galleries.The entire trunk was cut into two sections (upper section and lower section) from the middle; and the number and length of all egg galleries over the surface of each log were recorded.The bark beetles within galleries were also collected and distinguished with a binocular microscope.The heights and diameters of all the cut trees were measured.

Data Presentation and
Analyses.Data are presented as mean ± standard error (±SE).The correlations between T. yunnanensis and T. minor were calculated with bivariate methods.These analyses were conducted using SPSS 11.5 (SPSS Inc., Chicago, IL, USA).

Egg Gallery Distribution in Trunks.
Most T. yunnanensis began to move from shoots to trunks in November (Figure 1), and most T. minor began in December (Figure 2).In November, there were fewer T. minor egg galleries compared with T. yunnanensis, and all of the T. minor egg galleries were distributed in the lower half of the tree's trunk (Figure 1).T. yunnanensis egg galleries were distributed over the entire trunk in some trees, but with different densities in different sections.The highest density of T. yunnanensis was in the upper half of the tree trunks and was about 198 galleries/m 2 , while the highest density of T. minor was only about 19 galleries/m 2 .
In December, the attack density of T. yunnanensis and T. minor was in both higher than in the previous month, with T. minor increasing significantly (Figure 2).However, in the lower section of the trunks, the attack density of T. yunnanensis decreased a little compared to earlier.T. minor was still mostly distributed in the lower section of the trunk.
In January, the attack density (egg galleries) of T. yunnanensis remained highest in the upper section of the trunks and also remained low in the lower section of the trunk (Figure 3).The highest gallery density of T. yunnanensis was about 297 galleries/m 2 .The attack density of T. minor increased further, especially in the lower section of the trunks; and the egg galleries of T. minor were now distributed nearly over all areas of the trunks (Figure 4).The highest attack density of T. minor was about 305 galleries/m 2 .The measurements of egg gallery lengths show that in November these lengths for T. yunnanensis and T. minor were relatively short (Figure 5); T. yunnanensis egg galleries were about 3.6-4.9cm long, while T. minor's egg galleries were about 3.2-4.3cm long.In December, the egg gallery length increased, especially for T. minor (Figure 6).The egg galleries of T. minor were about 5.9-6.2cm long, and T. yunnanensis egg galleries were about 4.6-6.0cm long.In January, egg gallery length continued to increase (Figure 7), T. minor egg galleries lengthened to about 8.2-8.9 cm, and T. yunnanensis went up to about 5.2-6.6 cm long (Figure 8).

Bark Beetle Distribution in Tree
Crowns.About 11 T. yunnanensis were found in shoots per tree in November, and this number decreased to about nine by January, but the differences were not significant.Fewer T. minor (about four) were found per tree in November, and the number appeared to increase to five in December and then returned to four in January, but these differences were not significant.

Relationship between T. yunnanensis and T. minor.
The two bark beetle species had different relationships in different parts of the tree during the November to January period Average gallery length (cm)

T. yunnanensis T. minor
Tree height (m) (Figures 4 and 8).In November, there was no significant correlation between the two bark beetle species either with respect to trunks or to shoots (Table 1).In December, the two bark beetle species in shoots had no significant correlation either, but there was a significant negative correlation (P ≤ 0.01) between T. yunnanensis and T. minor in trunks (Table 1).In January, the two bark beetle species had significant negative correlations in shoots and in trunks (Table 1).

Discussion
The appearance of bark beetle galleries show that most T. yunnanensis begin to transfer from shoots to trunks in November, a little earlier than T. minor.Most of T. minor begin to move in December.This result is consistent with earlier reports [15,16].The transferring period lasts from November to January, or even longer, and there is no clear peak in transferring time.In Yunnan province, the weather is much drier than other times in winter from November to April.When winter approaches, the weather has little rainfall and the soil becomes dry.Some trees are weakened because of the shortage of water.These weak trees are the first choice for bark beetles to attack and feed on.As dry weather conditions persist, more and more trees are weakened and become suitable for bark beetles to colonize.At the study site, the colonized trees were cut down when they were found.However, as the colonized trees were cut down, newly colonized trees could always be found until February.When T. minor begins to transfer from shoots to trunks, and the density of T. minor increases in the lower trunk, the newly colonizing T. yunnanensis will no longer feed on lower sections, indicating the latter species is avoiding competition with T. minor (Figure 4).Thus, the colonized trees cut down later were found to have fewer T. yunnanensis in their lower sections.Similarly, T. minor were found mainly in the lower trunk in areas with less T. yunnanensis, thus both species T. yunnanensis (November) T. minor (November) T. yunnanensis (December) T. minor (December) T. yunnanensis (January) T. minor (January) appear to be avoiding competition by selecting areas of the host with lower densities of the opposite species (Figure 4).The egg gallery length of T. yunnanensis and T. minor not only increased during the three-month period, but also changed with the height of the trunks (Figures 5-8).The egg gallery length of T. yunnanensis increased, and the egg gallery Psyche 5 length of T. minor decreased with the height of the trunks in different months.
We found that there are mainly two ways for T. yunnanensis and T. minor to transfer from shoots to trunks.If the host trees were already weakened, the beetles would feed on the host tree trunks directly; if the host trees were initially more healthy, beetles would feed together on the shoots of the tree to weaken it and then transfer to the trunks of these trees.These insects use this strategy to weaken the tree's defensive ability and allow better survival of the individual bark beetles.
The relationship between T. yunnanensis and T. minor changed with time and location on the tree.When the time for transferring came, T. yunnanensis would feed on the top of the host tree's trunk to make their host weaker or to kill it.Occasionally we found live trees with a dead top that had been killed by T. yunnanensis; this indicates that host trees generally have a strong resistance and were not easy to kill [17,18], but when T. minor joined in to feed on the lower section of the host trunk, the resistance of the tree was further weakened which often led to its death.It is well known that the egg galleries of T. minor are perpendicular to the trunk length [19].This would probably cut more transporting tissues and might be more harmful to trees than T. yunnanensis which have galleries aligned with the trunk.In November, the attack density was relatively low in trunks, especially for T. minor, so the relationship between the two bark beetle species was predominately cooperation.With more and more bark beetles transferring to trunks; however, the competition between the two species intensified, and the cooperation between them diminished.However, they could coexist very well since both species had a separate colonization location on the trunk to avoid competition as much as possible.In fact, we scarcely found two egg galleries of different families joined together, no matter whether the galleries were from the same or the other species, as has been observed in other species [12,13,20,21].When two egg galleries almost touched, bark beetles would not proceed ahead, and in most of the cases, T. yunnanensis would change the egg gallery direction or abandon their egg gallery, thus making some egg galleries of T. yunnanensis not straight or with deviations.T. minor also would stop when closely approaching another egg gallery and often turned and excavated in the opposite direction, because there are only two directions for T. minor to bore their egg galleries perpendicular to the grain of the trunk.
In the tree crowns, the behavioral relationship between the two species was more complicated.It was not uncommon that two or three bark beetles tunneled in one shoot.But in most cases, the beetles were of the same species and usually consisted of one male and one female, or one male and two females.This suggests that there was competition between T. yunnanensis and T. minor in shoots.At the beginning of the transferring period, their relationships were dominated by competition in shoots; but with more and more bark beetles transferring from shoots to trunks, their relationship changed.In other words, the competitive relationship between the beetles changed as they transferred from branches to trunks.Thus, the competitive relationship between T. yunnanensis and T. minor occurs during their entire life cycle but alters in intensity and type with the life phase and location on the host tree.Knowledge about the movement from shoots to trunks (transferring) and trunk colonization process, as well as competition between the two species, will be useful in designing control strategies that take advantage of the vulnerability of the beetles and maximize resistance of the tree.

Figure 1 :
Figure 1: Average attack (egg gallery) density (±SE) and distribution of T. yunnanensis and T. minor along the trunks of Yunnan pines in November, 2005 (Qujing, Yunnan province, China).

Figure 2 :
Figure 2: Average attack (egg gallery) density (±SE) and distribution of T. yunnanensis and T. minor along the trunks of Yunnan pines in December, 2005 (Qujing, Yunnan province, China).

Figure 3 :
Figure 3: Average attack (egg gallery) density (±SE) and distribution of T. yunnanensis and T. minor along the trunks of Yunnan pines in January, 2006 (Qujing, Yunnan province, China).

Figure 4 :
Figure 4: Progression of average attack (egg gallery) density and distribution of T. yunnanensis and T. minor along the trunks of Yunnan pines from November to January, 2005-2006 (Qujing, Yunnan province, China).

Figure 8 :
Figure 8: Progression of average egg gallery length of T. yunnanensis and T. minor along the trunks of Yunnan pines from November to January, 2005-2006 (Qujing, Yunnan province, China).

Table 1 :
The correlation between T. yunnanensis and T. minor in trunks and shoots of Yunnan pines.