Recent Advance in Source, Property, Differentiation, and Applications of Infrapatellar Fat Pad Adipose-Derived Stem Cells

Infrapatellar fat pad (IPFP) can be easily obtained during knee surgery, which avoids the damage to patients for obtaining IPFP. Infrapatellar fat pad adipose-derived stem cells (IPFP-ASCs) are also called infrapatellar fat pad mesenchymal stem cells (IPFP-MSCs) because the morphology of IPFP-ASCs is similar to that of bone marrow mesenchymal stem cells (BM-MSCs). IPFP-ASCs are attracting more and more attention due to their characteristics suitable to regenerative medicine such as strong proliferation and differentiation, anti-inflammation, antiaging, secreting cytokines, multipotential capacity, and 3D culture. IPFP-ASCs can repair articular cartilage and relieve the pain caused by osteoarthritis, so most of IPFP-related review articles focus on osteoarthritis. This article reviews the anatomy and function of IPFP, as well as the discovery, amplification, multipotential capacity, and application of IPFP-ASCs in order to explain why IPFP-ASC is a superior stem cell source in regenerative medicine.


Introduction
In knee arthroplasty, infrapatellar fat pad (IPFP) as an inflamed tissue must be resected to relieve pain, so obtaining infrapatellar fat pad adipose-derived stem cells (IPFP-ASCs) avoids the secondary damage to patients as compared with obtaining mesenchymal stem cells (MSCs) derived from bone marrow, synovium, and subcutaneous fat. IPFP contains a large number of stem cells (5:5 × 10 6 stem cells in 21 ml of IPFP), so more stem cells may be obtained from IPFP than from bone marrow [1].
Due to the presence of telomerase activity which can delay the aging process, IPFP-ASCs have strong proliferation capability and differentiation potential, namely, that IPFP-ASCs can be purified by multiple passages and then show stable differentiation potential [2]. Moreover, IPFP-ASCs are more superior in chondrogenic differentiation and osteogenic differentiation than bone marrow mesenchymal stem cells (BM-MSCs) [3] and synovial fluid mesenchymal stem cells (SF-MSCs) [4]. Due to IPFP anatomical position, the surface markers of IPFP-ASCs are similar to those of other cells around the knee joint, so IPFP-ASCs can be implanted in the knee joint with other synthetic grafts to reduce immunologic rejection [5]. IPFP-ASC is a good seed cell for autologous or allogenic stem cell transplantation because of its strong proliferation capability and weak immunologic rejection [6,7].
IPFP, a subsidiary tissue to protect and support the knee joint, may have repair effects on the knee joint by secreting cytokines [8]. Currently, it is difficult for osteoarthritis to repair knee joint tissue and control disease progression due to weak regeneration capability and reduced blood supply [5], so the treatment for osteoarthritis focuses on relieving pain and protecting remaining joint function. In recent years, it is reported that autologous stem cell transplantation has a certain therapeutic effect on osteoarthritis [9]; SF-MSCs can repair cartilage, mesenchymal stem cells (MSCs) can relieve inflammatory pain by secreting prostaglandin E2 (PGE2) regulating the expression of inflammatory factors in surrounding cells, and the injection of supernatant fluid after IPFP-ASC culture into the knee joint can relieve inflammatory pain and restore partial joint function [10]. Therefore, IPFP has both physical protection effect and repair effect provided by paracrine secretion on the knee joint.
Scaffolds are usually used to improve stem cell molding growth. Compared with BM-MSCs and subcutaneous adipose-derived stem cells (Sc-ASCs), IPFP-ASCs grow better in the environment with scaffolds [11]. It is reported that IPFP-ASCs have a good application future in 3D bioprinting [12]. Therefore, IPFP-ASCs are more suitable to molding culture.
IPFP-ASC has become a focus in regenerative medicine and tissue engineering because IPFP-ASCs can be differentiated into bone, cartilage, and fat. Therefore, we reviewed the anatomy and function of IPFP, as well as the discovery, amplification, multipotential capacity, and application of IPFP-ASCs in order to explain why the IPFP-ASC is a high-quality source of stem cells for regenerative medicine. The review article provides a reference for clinical surgical treatment and regenerative medicine.

Anatomical Structure and Physiological
Function of IPFP IPFP, yellowish adipose tissue, is located among the patellar tendon, femoral condyle, and tibial plateau with a triangular shape. The tip of the IPFP is attached to the front of the intercondylar fossa, the bottom of the IPFP is below the basis patellae and on both sides of the patellar tendon, and the partial bottom is between the two-layer synovium. IPFP is an inverted triangle in shape, and the two angles of IPFP are located on both sides of the patellar tendon; the inner side of IPFP exceeds the medial margin of the patellar ligament about 2.5 cm, and the outer side of IPFP exceeds the lateral margin of the patellar ligament about 2.4 cm; IPFP stretches about 2 cm upward and about 1 cm outward, and IPFP finally is closely attached to the patellar medial inferior and lateral inferior sides, respectively [13]. In knee bend, IPFP enters the joint cavity to fill the empty space; while in strong contraction of the quadriceps femoris, the IPFP plays a role in preventing knee joint hypermobility. However, once IPFP hardens, IPFP may grow into the joint cavity and cause the limitation of joint motion. The blood supply of IPFP is mainly from the arteria genu inferior medialis and arteria genu inferior lateralis. The main physiological function of IPFP is to reduce friction and protect knee joint during joint motion. IPFP can prevent patellar injury by stabilizing patella and reducing friction [14]. Takatoku et al. [15] have found that IPFP is very important for patellar formation and development in immature rabbits. Besides cushioning shock and reducing friction, IPFP has a fixed effect on joint movement. IPFP also has repair effects on the injured knee joint because IPFP-ASCs in IPFP can differentiate into cartilage and can secrete cytokines such as platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), and fibroblast growth factor (FGF), which play antiapoptotic roles along with transforming growth factor-β (TGF-β).

Comparison of IPFP-ASCs with Other MSCs
Since Zuk et al. [16] discovered and cultured processed lipoaspirate (PLA) cells in 2001, a great deal of attention has been paid to adipose-derived stem cells (ASCs). In 2003, Wickham et al. [17] isolated stromal cells which can be differentiated into bone, cartilage, and fat from IPFP for the first time, providing a basis for investigating MSCs. The application of MSCs in tissue repair has become a hot issue in regenerative medicine and has attracted a great deal of attention from many research studies. Therefore, it is very important to choose a superior stem cell source. MSCs are mainly derived from bone marrow, synovium, subcutaneous adipose tissue, and IPFP. The MSCs derived from various sources are different in differentiation potential, proliferation rate, and surface markers. The surface markers are similar between IPFP-ASCs and BM-MSCs, but different surface markers between IPFP-ASCs and BM-MSCs are also present in some samples, which may be related to the different physiological status of samples and different culture conditions of stem cells.
Garcia et al. [4] have reported that the proliferation capability of SF-MSCs is weaker than that of IPFP-ASCs. Compared with Sc-ASCs, the differentiation capability is stronger and the cell volume is smaller in IPFP-ASCs [12,18]. CD34 and CD45 expression levels are higher in IPFP-ASCs than in BM-MSCs; and cartilage gene expression levels, especially SOX9 and COL2A1, are more active in IPFP-ASCs than in Sc-ASCs and Wharton's jelly stem cells (WJSCs) [5]. The above studies suggest that IPFP-ASC has marked advantages in proliferation and differentiation, especially in chondrogenic differentiation, and its strong proliferation capability provides a basis for subsequent large-scale studies. Culture efficiency and surface markers of MSCs from different sources are shown in Table 1.

Differentiation Potential of MSCs from Different Sources
Associated with the Growth Microenvironment and the Paracrine of Surrounding Cells. There is a lot of dense connective tissue around IPFP; but around Sc-ASCs, there is a great deal of subcutaneous tissue. The different growth microenvironments and paracrine of surrounding cells influence their proliferation capability and differentiation potential [26]. IPFP-ASCs can maintain stable chondrogenic differentiation potential during proliferation [3]. In addition, different cytokines, culture methods, and growth microenvironments are associated with IPFP-ASC differentiation potential, which will be introduced in detail in Section 4.3 of this article.

Proliferative Stability of MSCs from Different Sources
Probably Determined by the Integrity of Telomere. Telomere is a nucleoprotein located in the terminal end of the eukaryotic chromosome, and its length is regarded as a biomarker of aging. The maintenance of telomerase activity suggests that the cells have antiaging ability [27]. Until the 18th passage, IPFP-ASCs still have a growth rate and adipogenic differentiation potential which is confirmed by peroxisome proliferator-activated receptor γ2 and lipoprotein 2 Stem Cells International lipase [28]. This may be that IPFP-ASCs possess telomerase activity and telomere integrity, which allow IPFP-ASCs to have mitotic potential after multiple passages [2]. These findings prove IPFP-ASC proliferation capability and also provide an idea for future studies.

Effects of Different Physiological Conditions on
Proliferation Capability and Differentiation Potential of MSCs. In different physiological conditions, IPFP-ASCs have different proliferation capability and differentiation potential. In some knee joint diseases such as localized nodular synovitis, Hoffa's disease, or arthrofibrosis, the physiological function of MSCs from IPFP with different involvement levels is various. Local anesthetics can weaken the adipogenic differentiation potential of IPFP-ASCs [29]. The chondrogenic differentiation potential of IPFP-ASCs is stronger in obese donors than in thin donors [30]. The osteogenic differentiation potential of IPFP-ASCs is stronger in rheumatoid arthritis than in osteoarthritis, which may be related to tissue necrosis factor-α (TNF-α) [31]. Osteoarthritis can affect the chondrogenic differentiation potential of IPFP-ASCs [32]. The decreased chondrogenic differentiation potential of IPFP-ASCs may be associated with the downregulation of TGF-β gene expression in osteoarthritis [33]. It remains to be further investigated whether the IPFP-ASCs with stronger chondrogenic differentiation potential can be obtained by gene knockout.

Various Differentiation Potential and Proliferation
Capability of MSCs from Bodily Different Parts. Oxygen tension can promote the proliferation of BM-MSCs and IPFP-MSCs [34]. Suprapatellar fat pad adipose-derived stem cells (SPFP-ASCs) can specifically express CD105; SPFP-ASCs have stronger chondrogenic differentiation potential and better effects on relieving the symptoms of osteoarthritis in rat models than IPFP-ASCs [35]. Adiposederived stem cells isolated from lipoaspirates have stronger chondrogenic differentiation potential as compared with IPFP-ASCs [36]. Amaral et al. [37] have reported that cartilage cells differentiated by SF-MSCs are more stable and functional than those differentiated by IPFP-ASCs in vitro; but after subcutaneous injection of the two kinds of stem cells, there is no significant difference in differentiation potential between SF-MSCs and IPFP-ASCs in vivo. Above studies suggest that besides growth microenvironment, stem cell differentiation potential and proliferation capability are also related to stem cell sources and culture methods. In order to understand their interactions, it is necessary to further study the physiological process of stem cell proliferation.

Isolation and Culture of IPFP-ASCs.
Obtaining IPFP-ASCs requires a series of steps, including separation, mechanical digestion, chemical digestion, purification, and plastic attachment. The process includes shearing, collagenase digestion, impurity removal, filtration, centrifugation, supernatant removal, and transfer culture. The usage of collagenase is not uniform. It has been reported that usage of 0.075% collagenase I requires 2 h for digestion [38] and 0.1% collagenase I combined with one percentage of bovine serum containing CaCl 2 requires one hour for digestion [9], while there also is the usage of 0.25% trypsin which requires 20 min for digestion [31]. One study indicated that if 0.2% collagenase I was used for 10 min, the vascular matrix component could be obtained and if the 0.2% collagenase I was used for more than 30 min, the number of live adipocytes was reduced [39]. The addition of trypsin to collagenase I can accelerate the enzyme digestion. In future research, it is necessary to select the best type and concentration of enzyme to isolate stem cells quickly and efficiently. The common Sc-ASCs are derived from the liquid fat of subcutaneous tissue, so the liquid-dispersed morphology is conducive to obtaining stem cells; while IPFP-ASCs are derived from the solid fat in IPFP, so the solid fat must undergo physical shearing and chemical digestion into liquid fat, which may affect the time to obtain stem cells and the quantity and proliferation capability of stem cells. Recent studies have shown that human second passage IPFP-ASC into the third passage IPFP-ASC requires approximately 48-60 h [26,36,38]. The total number of cells has a certain effect on cell proliferation capability, which may be related to intercellular communication and microenvironment [40,41]. We should pay attention to these factors in order to  3 Stem Cells International obtain more stem cells with strong proliferation capacity. Francis et al. [39] found some optimized isolation methods such as frequent stirring, increasing collagenase, and using cell-specific coating flasks, which could obtain MSCs within 85 min and greatly shorten the time to obtain MSCs as compared with the previous 24-48 h. This makes it possible for time to use IPFP-ASCs in tissue transplantation of surgical emergencies. However, real application of IPFP-ASCs in acute trauma and transplantation surgery still requires a large number of clinical studies.
The culture conditions of IPFP-MSCs are similar to those of ordinary MSCs and commonly include 37°C, 5% CO 2 , and saturated humidity. After primary culture, trypsin digestion is used for serial subcultivation.

Identification of IPFP-ASCs.
There are many methods for the identification of stem cells. At present, the common identification methods include histology, immunohistochemical staining, qPCR, biochemical analysis, and image and mechanical detection, among which, the most commonly used method is immunohistochemical staining.
In 2006, the International Society for Cellular Therapy (ISCT) gave the definition of multipotential stromal cells and the bases to distinguish the multipotential stromal cells from hematopoietic stem cells as follows: (1) cell adhesion growth in vitro; (2) positive expression of CD44, CD73, CD90, and CD105 (positive detection rate of flow cytometry > 95%) and negative expression of CD11b, CD14, CD19, CD34, CD45, CD79α, and HLA-DR (the positive detection rate of flow cytometry < 2%); and (3) having the capabilities to differentiate into osteoblasts, adipocytes, and cartilage cells in vitro in specific culture conditions. IPFP-ASCs are not good for staining using low-affinity nerve growth factor receptors or STRO-1 [42]. In addition, IPFP-ASCs have higher expression levels of CD34, CD45, and CD166 than BM-MSCs, which suggests that IPFP-ASCs are different from BM-MSCs, but both still have similar surface markers such as CD73, CD90, and CD105 [26]. In the posttenth passage IPFP-ASC surface, there is the expression of CD13, CD44, CD90, and CD105 and occasionally the expression of 3G5, but no expression of LNGFR, STRO-1, CD34, and CD56 [28]. In the third to fifth passage IPFP-ASCs, the expression of CD13, CD45, and CD43 is negative and the expression of CD29, CD44, and CD90 is positive [43]. A systematic study on IPFP-ASCs shows that the surface antigens of IPFP-ASCs are CD9+, CD10+, CD13+, CD29+, CD44+, CD49e+, CD59+, CD105+, CD106+, and CD166+ [21]. So far, no specific markers have been found during IPFP-ASC culture, which may be related to the coexistence of multiple differentiated directional cells, so it is necessary to screen the unique target cells [26]  Adjusting the inorganic culture environment by changes of biophysical factors may obtain different culture efficacies. Hydrostatic pressure plays an important role in IPFP-ASC chondrogenic differentiation [34]. Intermittent hydrostatic pressure may have a stronger effect on the chondrogenic differentiation of cartilage progenitor cells than on the chondrogenic differentiation of IPFP-ASCs [44]. Hypoxia (5%) is conducive to IPFP-ASC chondrogenic differentiation but can decrease IPFP-ASC osteogenic differentiation in mice [45]. Hyaluronic acid is an important extracellular matrix for chondrogenesis. The culture medium containing 25% hyaluronic acid is very conducive to IPFP-ASC chondrogenic differentiation, but culture medium containing 25% to 75% hyaluronic acid has no effect on the proliferation capability of IPFP-ASCs [5]. The cartilage similar to the structure and function of natural cartilage can be obtained by controlling the oxygen concentration of hydrogel and the surrounding environment [46]. The chondrocyte-laden hydrogels supported by a superficial layer of stem cells allow MSCs to have stronger chondrogenic differentiation potential, suggesting that the orderly environment is more conducive to MSC differentiation [38]. The recent studies on chondrogenic differentiation of MSCs and IPFP-ASCs are shown in Table 2.
Because IPFP is near the synovium and cartilage, IPFP-ASCs in IPFP may be induced to differentiate into chondrocytes by the growth factor secreted by its surrounding cells [21]. An in vivo 3D culture of IPFP-ASCs indicates that the cartilage matrix scaffold treated by TGF-β3 and the gelatum treated by TGF-β1 promote IPFP-ASC chondrogenic differentiation [57,58]. The coculture of IPFP-ASCs with articular cartilage cells and hyaluronic acid can promote IPFP-ASC chondrogenic differentiation and prevent the occurrence of hypertrophic articular cartilage [48]. Above studies suggest that there are close communications between cells and the surrounding environment.
In addition, there are studies on IPFP-ASC differentiation at gene level. Bravo et al. [32] have found that during IPFP-ASC chondrogenic differentiation, the expressions of PTFR and MMP13 are similar between the patients with knee osteoarthritis and the individuals with healthy knees, but the expressions of OPG, FGF2, TGF, and MMP3 are significantly lower in the patients with knee osteoarthritis than in the individuals with healthy knees.

Osteogenic Differentiation.
Osteogenic differentiation is also an important research direction of IPFP-ASCs. Compared with chondrogenic differentiation, the studies about osteogenic differentiation mainly focus on gene regulation and external scaffold construction, but there are also a few studies about cytokines. Osteogenic differentiation potential 4 Stem Cells International Hypoxia enhances cell viability and the expression of chondrogenic markers, and cellular response is superior with polycaprolactone than with hyaluronic acid [53] 2017 Human IPFP-ASCs Indirect coculture with osteoarthritis-derived articular chondrocytes Promoting chondritic phenotypic recovery and IPFP-ASC chondrogenic differentiation in osteoarthritis [54] 2017 Human IPFP-ASCs Culture of ascorbic acid-treated IPFP-ASCs The hardness of matrix is unchanged, but the chondrogenic differentiation potential is enhanced [55] 2017 Human IPFP-ASCs and Sc-ASCs 2003 Human IPFP-ASCs Embedded with fibrin glue followed by culture in cartilage medium Occurrence of chondrogenic differentiation after 6-week culture [1] ASCs: adipose-derived stem cells; MSCs: mesenchymal stem cells; IPFP-ASCs: infrapatellar fat pad adipose-derived stem cells; BM-MSCs: bone marrow mesenchymal stem cells; Sc-ASCs: subcutaneous adipose-derived stem cells; RHEB: ras homolog enriched in brain; TGF-β3: transforming growth factor-β3; ECM: extracellular matrix.

Stem Cells International
is stronger in the IPFP-ASCs from obese mice than in the IPFP-ASCs from emaciated mice [67]. Osteogenic capability of IPFP-ASCs is not significantly different from BM-MSCs [3] and SF-MSCs [4]. IPFP-ASCs only produce bone precursor protein when induced by the culture medium containing vitamin C and inorganic phosphorus, but bone formation protein-2 (BMP-2) can induce IPFP-ASC osteogenic differentiation [1]. After coculture of fetal cartilage with IPFP-ASCs on polycaprolactone scaffold, the expressions of type II collagen and polyproteoglycan significantly decrease, but Knocking out FAK gene Promoting osteogenic differentiation [69] 2018 Human Sc-ASCs AtECM Reducing the activity in early osteogenesis [70] 2017 Human IPFP-ASCs Coculture with fetal cartilage on polycaprolactone scaffold Reduced type II collagen and polyproteoglycan and increased Indian hedgehog factor [68] 2017 Human IPFP-ASCs RHEB overexpression in IPFP-ASCs Promoting osteogenic differentiation [56] 2016 Human ASCs from Life Technology (USA) MSCs cultured in 20%, 40%, and 60% FB scaffolds Promoting osteogenic differentiation, and osteogenic differentiation is more obvious in 40% and 60% FB scaffolds [71] 2016 Human ASCs from Life Technology (USA) Scaffolds with large stiffness and small aperture Osteogenesis is preferential [71] 2016 Mice Sc-ASCs (inguinal fat pads) The 3 rd to 12 th passage Sc-ASCs Increased osteogenic differentiation [6] 2016 Mice Sc-ASCs (inguinal fat pads) Low-density sowing Toward osteogenic differentiation [6] 2015 Human Sc-ASCs Osteogenic induction medium containing GLP-1 Promoting osteogenic differentiation [72] 2014 Rat Sc-ASCs (inguinal fat pad) Medium containing rhPDGF-BB Promoting osteogenic differentiation [73] 2014 Human Sc-ASCs Amplification on BM-ECM Producing more osteoblasts [74] 2014 Human Sc-ASCs Subcutaneous implantation of MSCs induced by BM-ECM in nude mice Producing more bone tissue [74] 2014 Human ASCs from Lonza (Basel, Switzerland) Addition of fullerol Improving osteogenic potential [75] 2013  Stem Cells International the expression of Indian hedgehog factor significantly increases, suggesting that children cartilage and polycaprolactone have strong osteogenic effect and weak chondrogenic effect on IPFP-ASCs [68]. The recent studies on osteogenic differentiation of ASCs and IPFP-ASCs are shown in Table 3.

Applications of IPFP-ASCs and MSCs
IPFP-ASCs are generally considered to be more promising stem cells because IPFP as an inflamed tissue must be resected to relieve pain in knee arthroplasty, so obtaining IPFP-ASCs avoids the damage to patients as compared with obtaining bone marrow-derived MSCs, and IPFP-ASC isolation procedure is easy to operate. The injection of IPFP-ASCs can effectively relieve pain and improve knee joint function in osteoarthritis [100,101]. Koh et al. [100,101] reported that after injection of platelet-rich plasma containing a mean of 1:89 × 10 6 IPFP-ASCs into arthritic knees, the mean Lysholm, Tegner activity scale, and VAS scores improved significantly and no major adverse events related to the injections were observed; the therapeutic effects were positively associated with the number of IPFP-ASCs but negatively associated with disease duration and patient's age. IPFP-ASCs show marked therapeutic effects in young patients and patients with early cartilage deformation [98,99]. The chondrogenic differentiation potential of IPFP-ASCs from the patients with advanced OA is decreased [102]. IPFP-ASCs have good effects on osteoarthritis in stages I and II [103]. IPFP-ASCs may be induced to produce musclespecific cytoskeleton proteins which are conducive to muscle regeneration [9].
Besides subpatellar fat pad, MSCs are also obtained from subgluteal fat pad, abdominal liposuction, and inguinal fat pads. MSCs from these different sources all show good functional characteristics. The postoperative evaluation is better in BM-MSC transplantation combined with microfracture than in microfracture alone [104]. The outcomes of subgluteal fat pad-derived MSC implantation for cartilage repair of osteoarthritic knees seem encouraging [105]. The graft survival and the volumes of fat and newly generated connective tissue all are significantly higher in abdominal liposuction-derived MSC-enriched fat grafts than in fat grafts alone, suggesting that MSC-enriched graft could render lipofilling a reliable alternative to major tissue augmentation, such as breast surgery or major flap surgery [106]. A combination of percutaneously injected autologous adipose tissuederived stem cells, hyaluronic acid, platelet-rich plasma, and calcium chloride may regenerate bone and cartilage in the patients with hip osteonecrosis or knee osteoarthritis [107]. The applications of IPFP-ASCs and MSCs are shown in Table 6.
At present, most studies on IPFP-ASC are about in vitro IPFP-ASC culture and IPFP-ASC animal experiment. In recent years, 3D printing technology has solved the nonmolding problem of stem cell differentiation and can construct complex human structure and improve cell differentiation efficiency and/or posttransplantation survival ability. Stem cells may be differentiated into cartilage by microfluidics without the requirement of growth factors [12]. With the development of stem cell technology and biomaterial science, IPFP-ASCs will be more and more widely used.

Summary and Outlook
Compared with other MSCs, IPFP-ASCs have the following characteristics: (1) IPFP-ASC isolation is relatively easy, and more stem cells may be obtained from the IPFP. (2) IPFP-ASCs have strong proliferation capability and differentiation potential, and IPFP-ASCs are more superior in chondrogenic differentiation and osteogenic differentiation than BM-MSCs and Sc-ASCs. (3) IPFP-ASCs can secrete a variety In summary, many problems on IPFP-ASCs remain to be solved, but more and more attention has been paid to it because IPFP collection is easy, IPFP contains a large number of stem cells, IPFP-ASC isolation is simple, and IPFP-ASCs have multidirectional differentiation potential. IPFP-ASCs will be a promising stem cell source in regenerative medicine after constantly overcoming the existing problems such as optimizing pretreatment stem cell immune specificity and homogeneity, improving IPFP-ASC differentiation capacity into target cells, exploring the best transplantation stage,  [108] 2018 Human Osteoarthritis Sc-ASCs Injection of Sc-ASCs into knee joint cavity Reducing cartilage defects by regeneration of hyaline-like articular cartilage [109] 2018 Human Osteoarthritis Sc-ASCs Injection of PRG into knee joint cavity Relieving pain [11] 2018 Human In vitro model of meniscal tear

Sc-ASCs
Transplantation of three-dimensional graft from autologous Sc-ASCs and decalcified bone matrix into knee joint Promoting osteogenesis in bone nonunions and restoring anatomic structure without oncological side effects [112] 2014 Rabbit Osteoporosis Sc-ASCs Injection of Sc-ASCs into knee joint cavity Promoting bone regeneration in vivo and relieving osteoporosis [113] 2014 Rat Abdominal aortic aneurysm

Sc-ASCs
Injection of Sc-ASCs into abdominal aortic aneurysm Promoting the expression of elastin in smooth muscle and facilitating the reconstruction of elastic membrane [114] 2012 Human Osteoarthritis IPFP-ASCs Injection of IPFP-ASCs into knee joint cavity Effectively relieve pain and improve the function of knee joint [99] 2011 Human Calvarial defects Sc-ASCs Transplantation of Sc-ASCs with Noggin knockout into the destroyed skull in mice Rapidly repairing mouse calvarial defects [78] 2010 Mice Tibia destruction Sc-ASCs Transplantation of ASCs stimulated by N-terminal sonic hedgehog into tibial defect Enhancing osteogenesis [79] 2008 Pig Lumbar disc degeneration IPFP-ASCs Injection of IPFP-ASCs with rhBMP-6 overexpression into lumbar paravertebral muscle Spinal fusion and new bone formation [81] 2003 Human Osteoarthritis IPFP-ASCs IPFP-ASCs were embedded into fibrin glue nodules, for inducing chondrogenic cells, and were placed in osteogenic media containing BMP-2 for inducing osteogenic cells, respectively. And then these cells were transplanted into the hind legs of nude mice Cartilage formation induced by fibrin glue nodules, bone formation induced by BMP-2, and bone marrow cavity formation in the hind legs of the osteogenic culture group [1] IPFP-ASCs: infrapatellar fat pad adipose-derived stem cells; MSCs: mesenchymal stem cells; Ha-MSCs: human amniotic mesenchymal stromal cells; Progenza (PRG): comprises in vitro expanded mesenchymal stem cells derived from human donor adipose tissue combined with cell culture supernatant; TGF-β: transforming growth factor-β; rhBMP-6: recombinant human bone morphogenetic protein-6; BMP-2: bone morphogenetic protein-2. 9 Stem Cells International and increasing posttransplantation regeneration rate. We believe that the key problem resolved in the future is that the methods for IPFP-ASC directional differentiation will be safe, controllable, and manipulable.

Conflicts of Interest
The authors declare that they have no conflicts of interest.

Authors' Contributions
Yu-chen Zhong and Shi-chun Wang equally contributed to this work.