Nutrition in Herbal Plants Used in Saudi Arabia

Herbs are used for centuries by many people worldwide. This study derives insights into the use and content of herbs that are consumed among Saudi citizens. An online questionnaire was distributed to understand the basic information about Saudi citizens' preference and daily patterns of herbal plants that are usually used as drinks. Moreover, concentrations of fourteen elements in twenty-one herbal plants that were indicated in the previous questionnaire were collected from the local market and then analyzed using an Inductively Coupled Plasma Emission Spectrometry (ICP). Mint leaves were significantly higher in most of the nutrients analyzed, and mint was the most popular drink among participants, followed by green tea and anise. Most of the citizens preferred to drink one cup only at home and believed that herbs are good for their health and potentially could help them to sleep better. The outcomes derived from this research could help future assessments of diet patterns among Saudi citizens.


Introduction
Herbal plants, hereafter, herbs, are one of the most important sources of many elements that are vital for humans and animals' diet and are used to prevent or treat diseases. ese elements are necessary nutrients to their lives and are, therefore, needed in food as the body cannot synthesize them [1]. Herbs play an important role in politics, romance, religion, and health [2], and they are still used by millions of people globally, in particular as medicine as there are more than 50 elements considered as a major component of enzymes and proteins [3]. And optimal uptakes of nutrients may reduce health risk [4]; therefore, their use is increasing globally due to their low side effects when used moderately [5]. Hence, knowing the exact elemental composition of food and other products is important to understand the nutritive value [6], while the use of low nutritive refined food products may affect health [7]. Moreover, some of these are essential nutrients to a certain limit, e.g., iron and copper, whereas others, e.g., lead and cadmium, are poisonous [5].
Most of the herbs are naturally grown in many regions around the planet and used from ancient times [8]. ey are also used for culinary [9] cosmetics [10][11][12], and up to 80% of the world's population depend on plant-derived drugs for medical purposes [13]. Also, green tea is a good example of the use of herbs that started for centuries in China and Japan [6] and is still used nowadays. However, for safety reasons, it is advisable to assess different pollutants before delivering those herbs to the end user (consumer) [14].
Numerous attempts have been made to determine the element's content in plants around the world, e.g., Nigeria [15] and Malaysia [16]. However, a quick search in Scopus with the keywords "herbal plant * " and "nutrient * " revealed that most of the published papers are from Asia, i.e., India, China, Iran, and Malaysia. Saudi Arabia's market is rich with various herbal plants that citizens are using as "traditional medicine," many of those are coming from Asia and Africa, while the rest are harvested locally. Studies concerning elemental content of these herbs that are acquired from the Saudi market are rare, whereas herbal preference among citizens and their knowledge base have not hitherto been performed. As a result, the outcomes of this research would serve as a base for further assessments related to herbal plants in Saudi Arabia and describe patterns of practices among Saudi citizens.

Questionnaire.
To assess the public preference regarding herbal plants that are usually used on a daily basis by people in Saudi Arabia, a survey was carried out online in 2018. e participants were asked before starting the survey if they do drink herbal tea; if no, the survey was terminated. e panel respondents who were above 18 years old were invited to participate via Google survey online (https://gsuite.google.com/products/forms/). However, only 44 participants were under 18 which corresponds to 4% only; therefore, their answers were not excluded from the results. Demographic characteristics (i.e., questions about sex, age, and educational stage were asked to identify the range of participants) are shown in Supplementary  Table S1. e questionnaire was designed to include the following: From where do you get your herbs, why do you drink herbal tea, does it help you sleep, where do you drink it, how many cups per day, how many scoops of the herb do you use per cup, and which herbs do you drink, along with options to choose from which are in Supplementary  Table S2. e questionnaire was in the Arabic language; then, the results were translated to English.

Collection of the Samples.
Twenty-one herbal plants that were indicated in the previous questionnaire (Table 1 and Figure 1) were collected from a local market in Dammam, Saudi Arabia, in 2018. e material was already dried; therefore, three replicated samples (n � 3) from each herb were directly ground in an agate mortar. e scientific name and family of each herb were further investigated using Encyclopedia Britannica (http://www.britannica.com) and Herbal Encyclopedia (https://www.cloverleaffarmherbs.com).

Chemical Analysis and Data
Quality. Plant samples were already dried, then ground using a granite mortar, and photographed; then, 0.5 grams of the plant sample (i.e., leaves, stem, or seeds) was digested with 5 mL of concentrated HNO 3 and 2 mL of H 2 O 2 in polyethylene tubes at digestion systems for 2 hours at 100°C [17]. e digested samples were left to cool, then diluted to 45 mL with Milli-Q water (18.2 Ω/cm), then filtered with a Whatman filter paper of 44-micron size. Concentrations of fourteen elements: calcium (Ca), chromium (Cr), copper (Cu), iron (Fe), iodine (I), potassium (K), magnesium (Mg), manganese (Mn), molybdenum (Mo), sodium (Na), nickel (Ni), phosphorus (P), selenium (Se), and zinc (Zn), were analyzed using Inductively Coupled Plasma Emission Spectrometry (Shimadzu, model 9820). To confirm the quality of the analysis, replicates and PanReac AppliChem multielement standard solution between the 20 samples were used. e analytical recovery of the standards and the duplicated samples is reported in Table 1.

Statistical Analysis.
Descriptive statistics and general linear models were used to test the effects of differences among herbs for every single element as well as Tukey's HSD (honestly significant difference) post hoc test was used to assess pairwise differences. Moreover, assuming that the average consumption of herbs is one spoon equivalent of 0.5 g, the estimated dietary intake (mg day −1 ) based on 70 kg body weight was calculated by dividing the nutrient concentration (mg kg −1 ) by 70. All statistics were computed using JMP v12 (Table 2).

Results
e range of participants who answered the online survey that was distributed through the social media was from 12 to answered that they get it from their mothers or herb specialists, whereas 43% from the supermarket (Figure 2 and Supplementary Table S2).
Most of the elements analyzed, i.e., Ca, Cu, Fe, Cr, Mn, Ni, and Se, were significantly higher in mint, maidenhair, green tea, dried black lime, and laurel leaf whereas Zn, Na, and Mg were significantly higher in mugworts, and K was significantly higher in both mugworts and curcuma. Moreover, P, Mo, and I were significantly higher in lemon balm, dried black lime, and mint, respectively (Table 3, Tukey's HSD, P < 0.05).

Discussion
e Recommended Dietary Allowance (RDA) is the sufficient amount of required nutrients that are needed for healthy individuals, which are established by the Food and Nutrition Board [18]. Herbal plants are one of the major resources of these nutrients and, therefore, the amount of nutrients in plants is one of the criteria that makes it favorable to use [6], although the paucity of these nutrients can lead to disease [1]. Many of them are essential nutrients for humans; e.g., iron is basic to hemoglobin and many enzymes, while calcium and phosphorous are essential      RDA values from [20] Scientifica 7 components to bones, magnesium is important to all biosynthetic processes, and zinc is basic to many enzymes that are involved in metabolic pathways [19]. e estimated dietary intake (mg day −1 ) in this study compared to the RDA varied among different elements. at is, Ca, K, Mg, Na, P, and Zn were within the recommended range of intake for males and females (Table 4) while Cr, Cu, I, Mo, and Se were higher than the range of the reported values and finally Fe and Mn were intermediate with seven herbs (i.e., black lime, green tea, laurel leaf, maidenhair, mint, mugworts, and thyme; Table 4) out of twenty-one being higher than the RDA values reported by Dell Valle et al. [20].
Moreover, in this study, two-thirds of the participants were females, indicating a higher rate of use among females; this rate of female users was observed by the findings of Alghamdi et al. [21]. e growing interest of females to herbal drinks, e.g., tea, might be due to the daily news of their benefits that ranged from the protection of hip structure in elderly women [22] to cancer potential treatments and risks caused by diabetes [23].
While there is no explanation why the majority drink only one cup of herbal tea preferring it at home compared to the work place, and around half of the participants acquire it from their moms or specialists, yet, in general, about 79% of the participants believed that herbal tea is good for their health compared to smell and taste, and 83% believed that it may help them sleep. Similarly, a recent study on two hundred patients in Saudi Arabia found that 76% of the patients with chronic illness had used herbal medicine [21], compared to a lower range of 10 to 52% in western countries [24]. And a higher range of migrants of about 75% uses herbal medicine in the west [25].
Mint was the most popular herb among Saudi citizens, followed by green tea and anise. In Australia, mint comes as number seven of most sold herbs and spices in the supermarket for 2013 with about $2151500 of sales value [26]. However, many studies documented the effect of green tea as a potential treatment preventing cancer [27,28]. Furthermore, most of the nutrients, i.e., Ca, Cu, Fe, Cr, Mn, Ni, and Se, were significantly higher in mint, maidenhair, green tea, dried black lime, and laurel leaf, whereas nutrients' concentrations in this study, particularly, Zn, Na, K, and Mg, were significantly higher in mugworts compared to other herbs, which is coherent with early Chinese beliefs of the medical benefits of mugworts, as well as France in the middle ages, where they used it to protect babies from cold [2].
Nevertheless, these herbs must be used with care, as many reports have documented the presence of toxic heavy metals and undeclared pollutants, e.g., in Asia [29][30][31] and Africa [32,33].

Conclusion
e concentration of nutrients carried out was depending on the plant species analyzed; most of the herbs analyzed were within the RDA limits. However, participants who selected many cups per day or many spoons per cup could be at risk of bioaccumulation. In this study, mint leaves were significantly higher in most of the nutrients analyzed, and mint was the most popular drink among Saudi citizens, followed by green tea and anise.

Data Availability
e data used to support the findings of this study are included within the supplementary information files.

Conflicts of Interest
e author declares that there are no conflicts of interest.