Comparative and Correlational Evaluation of the Phytochemical Constituents and Antioxidant Activity of Musa sinensis L. and Musa paradisiaca L. Fruit Compartments (Musaceae)

Secondary metabolites and their biological activity have pharmacological relevance in the prevention and therapeutic management of disease, including the facilitation of normal physiological processes through biochemical mechanisms. In this study, phytochemical constituents and antioxidant activity were evaluated quantitatively on the acetone, ethanol, and aqueous extracts of the flesh, and peel, as well as the boiled peel extract compartments of Musa sinensis L. and Musa paradisiaca L. fruits. Total phenol, proanthocyanidin, and flavonoid contents were estimated and measured spectrophotometrically. The free radical scavenging antioxidant capacity of the extracts was tested on DPPH (2,2-diphenyl-1-picrylhydrazyl ethanol), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)), and FRAP (ferric reducing antioxidant power) assay models. Correlation between phytoconstituents and antioxidant activity was analysed using Pearson's coefficient. The results showed varying amounts of phytochemicals in the solvent extracts of the flesh and peel, including the boiled peel extract of M. sinensis and M. paradisiaca. All acetone extracts of M. sinensis flesh, M. paradisiaca flesh, and M. paradisiaca peel had the highest phytochemical contents, with the exception of the ethanol extract of M. sinensis peel which had the highest phenol content; just as on the overall scale, the peel compartments had generally higher phytochemical profiles than the soft flesh in both fruits. The boiled peel extracts of M. sinensis and M. paradisiaca had the highest ABTS (0.03 mg/mL) and DPPH (0.03 mg/mL) activity. Ferric reducing power (FRAP) was the highest in the ethanol extracts of M. sinensis flesh and peel, and M. paradisiaca flesh, while it was the highest in the acetone extract of M. paradisiaca at the peak concentration used (0.1 mg/mL). There was a significant negative correlation between the total phenol and flavonoid contents of M. sinensis flesh with its DPPH radical scavenging activity and proanthocyanidin content of M. paradisiaca flesh with its DPPH radical scavenging activity. The correlation outcomes indicate that none of the phytochemical constituents solely affected antioxidant activity; instead, a combination of the polyphenolic constituents contributed to antioxidant activity. This study shows the therapeutic potentials of the flesh and, importantly, the peel of M. sinensis and M. paradisiaca fruits on the basis of the polyphenolic constitution against free radicals and oxidative stress.


Introduction
e medicinal relevance of plants chiefly lies in their chemistry, and plant secondary metabolites, in particular, are the key natural products that essentially drive the pharmacological activities of plants. eir utility spans industrial production frontiers such as pharmaceuticals, nutraceuticals, and textiles. Medicinal plants are so regarded because they possess therapeutic properties and have active components useful for drug synthesis and relevant in phytomedicine. Alkaloids, quinones, coumarins, and flavonoid compounds have been implicated in pharmaceutical processes [1] and the prominence of herbal medicine in many underdeveloped and developing countries. Scientific articles report that plant extract used in phytomedicine has paved the way for improved therapeutic scientific insights towards the development of the empirical medicinal system [2]. Oxidative stress, which in principle alters the physiological dynamics and triggers equilibrium disruption, is linked to series of inflammatory diseases such as arthritis, gastric ulcers, vasculitis, and lupus erythematous, including protein and DNA damage. In this regard, antioxidants provide defence mechanism through the prevention of oxidative damage, which is a notorious tendency of free radicals [2]. ese defence mechanisms are expressed through enzymatic and nonenzymatic processes, some of which include the peroxidase, polyphenol oxidase, and the glutathione in its reduced form [3]. Antioxidants are peculiar in action because they have the capacity for repair, suppression of free radical formation, and scavenging. In biological systems, antioxidants express their mechanism through chelation of metal ion, regulation of gene expression, and the coantioxidants. Fruits are sources of many nonenzyme antioxidants like vitamins, gallic acid, rutin, and quercetin [4,5].
Phytochemical compounds are secondary metabolites naturally sourced from fruits, vegetables, and other plant products, amongst them are the phenolics, which are central to the signal and defence mechanism in plants. Polyphenols help in reducing the risk of neurodegenerative disease, leukaemia, vasorelaxation, and antiallergenic activity [6] as well as possessing the capacity to interact with antioxidant enzymes [7,8] such as glutathione S-transferases and the NADPH: quinine oxidoreductase (NQO1) and preventing the initiation phase of carcinogenesis through the modulation of cytoprotective enzyme activation [9]. e significance of dietary phytochemicals has been put forward in terms of being responsible for distinct plant characters like aroma and colour pigmentation [10]. It has also been reported that several phytochemicals are integral in affecting cell proliferation and regulation of cell cycle [10]. ey are active in a series of signalling pathways that are usually disrupted during tumour initiation and proliferation [11][12][13].
Banana and plantain, members of the Musaceae family (Musa spp.), are important food crops that supply energybased carbohydrates, including a wide spectrum of other nutritive components in human nutrition [14][15][16]. ey are tropical herbaceous plants that grow up to heights reaching 9 metres, and are produced largely in the Asian, African, and South American regions; sweet fruits in the case of banana and for plantain are popularly cooked for food [17]. ese plants have been implicated in agricultural and industrial uses, which make them valuable to the bioeconomy [18,19]. Banana and plantain fruits possess very thick coverings known as the peels; however, they have low dietary incorporation status as they constitute waste because they are usually disposed during the consumption of the fruit pulp. It is with the foregoing that this study has been designed to evaluate polyphenolic constituents and biological activity of extracts across the compartments (flesh, peel, and boiled peel extract) of M. sinensis and M. paradisiaca fruits, with an aim to establish the phytomedicinal viability of the often neglected peel and boiled peel extract component. In so doing, this study seeks to project the peels of M. sinensis and M. paradisiaca as potential functional foods with medicinal, therapeutic, and nutritional value. With the foregoing, this study has been designed for the evaluation of the pharmacological potential in the extracts of M. sinensis and M. paradisiaca fruits, which will further validate the dietary medicinal value of the soft flesh and particularly the less utilised peels of the fruits. Analytical grade reagents were used for the phytochemical and antioxidant experimental assays, and they include the Folin-Ciocalteau's (FC) reagent, aluminium trichloride (AlCl 3 ), anhydrous sodium carbonate (Na 2 CO 3 ), sodium hydroxide (NaOH), sodium nitrite (NaNO 2 ), sodium acetate, hydrochloric acid (HCl), 2,2diphenyl 1-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3ethylbenzthiazoline-6-sulfonic acid) (ABTS), potassium persulfate (K 2 ,S 2 ,O 8 ), ferric chloride (FeCl 3 ), vanillin, rutin, trichloroacetic acid (TCA), quercetin, catechin, gallic acid, and methanol.

Materials and Methods
Pulverization of soft flesh and peel samples was done with a food blender (HBF 500S series, Virginia, USA). e boiling of peel samples was done in a water bath (BUCHI B-480). Filtration of sample-solvent mixtures was done using a set-up of the Whatman filter paper, Buchner funnel, and vacuum pump. Aqueous extracts were chilled in a refrigerant chiller (PolyScience AD15R-40-A12E, USA). Freeze-drying of aqueous extracts was done with a freeze dryer (Ceramic Filter Core Drier CD 052), Oven-drying of soft flesh and peel samples was done in an oven (LABOTEC, Durban, South Africa). Homogenization of extracts was done on a shaker (Lasec Stuart Orbital Shaker SSL1); organic (acetone and ethanol) extracts were concentrated using a rotary evaporator (LABOROTA 4000 Heidolph). Absorbance spectra were measured with Hewlett Packard VR-3000 PC spectrophotometer, 765 nm.

Sample and Extract Preparation.
e fruits were well rinsed with distilled water, and the sliced soft flesh and the peel samples were oven-dried (LABOTEC, South Africa) at 40°C for 72 hours, after which the dried samples were pulverised using a food blender (Hamilton Beach HBF 500S Series). Another group of M. sinensis and M. paradisiaca peels (1000 g/L) were boiled in distilled water in the water bath (BUCHI B-480) at 80°C for 20 minutes. e boiled peel extracts were freeze-dried for 48 hours (Ceramic Filter Core 2 e Scientific World Journal Drier CD 052) and stored at 4°C until analytical work commenced. 150 g of the pulverised sample was weighed into three conical flasks, each containing 1,000 mL of acetone, ethanol, and distilled water solvents. Afterwards, the mixtures were homogenised constantly at 124 rpm on a shaker (Lasec Stuart Orbital Shaker SSL1) for 48 hours. e solvent mixtures were then pressure-filtered through the Whatman filter paper (No 1, 25 mm) in a Buchner funnel set-up on the vacuum pump. e acetone and ethanol filtrates were then concentrated further to obtain crude extracts using a rotary evaporator (LABOROTA 4000 Heidolph) at 56°C and 78°C [20][21][22] boiling points, respectively, while the aqueous filtrates were chilled in a refrigerant chiller (PolyScience AD15R-40-A12E, USA) at −40°C and concentrated to dry form using the freeze dryer for 48 hours (Ceramic Filter Core Drier CD 052). Water (aqueous) and ethanol extracts were prime organic solvent options since the medium of cooking and preparation of herbal medicines are infusion or decoction using water or alcohol, with acetone chosen because of its extractive ability for hydrophilic and lipophilic plant components. e extraction yields of the three solvents for the fruit compartments of M. sinensis and M. paradisiaca were evaluated and recorded. e extracts were stored at 4°C until further analysis.

Total Phenolic Content.
Total phenol was determined using Folin-Ciocalteau's reagent by the method described by [23] with slight modifications to the concentration. A 0.5 mL aliquot of the different extracts (1 mg sample/mL in methanol) and the gallic acid standard was mixed with 2.5 mL of 10% (v/v) Folin-Ciocalteau's reagent and incubated for five minutes. To the solution, 2 mL of 7.5% (w/v) anhydrous sodium carbonate (Na 2 CO 3 ) was added. e mixtures were incubated at 40°C for 30 minutes (LABOTEC-South Africa). Spectrophotometric measurement of absorbance was taken at 765 nm (Hewlett Packard VR-3000 PC spectrophotometer). Total phenolic (TP) content (mg GAE/g) was derived from the standard curve equation: y � 8.3093x − 0.0023, R 2 � 0.9992. is was expressed from the formula y � CV/m, where C is the total content of phenolic compounds in plant extract in mg GAE/g, V is the volume of plant extract used in the assay in mL, m is the mass of extract used in the assay in g, and y is the concentration obtained from the standard curve in mg/mL.

Total Proanthocyanidin Content.
is was determined based on the method described by [24] with slight modification. 0.5 mL of extracts and different concentrations (0.0625 mg/mL to 1 mg/mL) of catechin (standard) were dispensed into different test tubes. en, 3 mL of vanillinmethanol (4% w/v) and 1.5 mL of hydrochloric acid were added. e mixture was then vortexed and left to stand for 15 minutes at room temperature. e absorbance was spectrophotometrically measured at 500 nm (UV-3000 PC spectrophotometer). Total proanthocyanidin (TPR) content was evaluated using the equation of the calibration curve: y � 1.1493x + 0.0334, R 2 � 0.9839, and it was expressed as mg catechin equivalent (CE)/g using the formula, C � cV/m, where C is the total proanthocyanidin content in plant extract in mg CE/g, c is the concentration obtained from the standard curve (mg/mL), V is the volume of plant extract used (mL), m is the mass of extract used (g).

Total Flavonoid Content.
Total flavonoid content in the extracts was determined using the colorimetric aluminium chloride method described by [25]. 0.5 mL of plant extracts and varying concentrations of quercetin standard, ranging from 0.0625 to 1.0 mg/mL, were dispensed into different test tubes. Into tubes were added 2 mL of distilled water and 0.15 mL of 5% sodium nitrite (NaNO 2 ), with the solution made to stand for six minutes, after which 0.15 mL of 10% aluminium chloride (AlCl 3 ) was added and incubated for five minutes. e mixtures were vortexed and incubated at 25°C for 15 minutes (LABOTEC, Durban, South Africa), and 1 mL of 1M sodium hydroxide was added in each tube. e solution was made up to 5 mL with the addition of 1.2 mL distilled water. Absorbance was measured at 420 nm with a spectrometer. Flavonoid content was quantitatively derived using the equation of the calibration curve: where C is the total content of flavonoid compound in plant extract (mg QE/g), c is the concentration obtained from standard curve (mg/mL), V is the volume of plant extract used (mL), and m is the mass of extract used (g).

Antioxidant
Activity. e antioxidant capacity of the fruits (flesh, peel, and boiled peel extract) of M. sinensis and M. paradisiaca was evaluated using the DPPH, ABTS, and the FRAP assays.

DPPH Radical Scavenging
Activity. DPPH radical scavenging activity was determined as described by [26]. Briefly, 1 mL of 0.135 mM DPPH radical (prepared with methanol in an opaque bottle) was added to 1.25 mL of the extracts and standards (gallic acid and rutin), each at varying concentration levels (0.0025 mg/mL to 0.01 mg/mL). e solution was vortexed and kept in dark condition at 25°C for 30 minutes. Absorbance was spectrophotometrically measured at 517 nm with methanol as blank. Scavenging activity was evaluated as follows: where Abs (control) is the absorbance of DPPH radical + methanol, and Abs (sample) is the absorbance of DPPH radical + sample extract or standard.
e Scientific World Journal

ABTS Radical Scavenging Activity.
is was determined as described in [27]. e stock solution of ABTS was prepared by mixing 7 mM ABTS and 2.45 mM potassium persulfate (K 2 S 2 O 8 ) (1 :1) and was incubated in the dark at 25°C for about 18 hours in order to release its radicals (ABTS + ). e solution was diluted further by the mixture of 1 mL ABTS + with 50 ml methanol to obtain a working solution (absorbance 0.700 ± 0.0006 at 734 nm). Plant extracts and rutin standard solution of concentration (0.005 mg/mL-0.08 mg/mL) were reacted with 1 mL ABTS in a test tube and kept in dark condition for 7 minutes. e absorbance was determined at 734 nm against methanol (blank): where Abs (control) is the absorbance of ABTS radical-+methanol and Abs (sample) is the absorbance of ABTS radical +sample extract/standard.

FRAP Assay.
e ferric reducing power of M. sinensis and M. paradisiaca extracts was determined using the method described by [28]. e FRAP reagent was prepared upon the mixture of 2.5 mL 0.2 M phosphate buffer reagent (62.5% monobasic + 37.5% dibasic; pH 6.6) and 2.5 mL of 1% potassium ferricyanide (K 3 Fe(CN) 6 ). 1 mL of the standard rutin and plant extracts at varying concentration levels (0.025 mg/ mL-0.1 mg/mL) were added to the FRAP solution. is mixture was incubated in a water bath (BUCHI B-480) at 50°C for 20 minutes. Afterwards, 2.5 mL of 10% trichloroacetic acid (TCA) was added and the mixture was centrifuged at 300 rpm for 10 minutes; then, 0.5 mL 0.1% iron (III) chloride (FeCl 3 ), 2.5 mL of distilled water, and the 8.5 mL supernatant from the centrifuged solution (1 : 5:5) were mixed and incubated for 10 minutes, at room temperature. e ferric reducing ability of plant extracts (mg/g Fe (II) equivalent) was measured at 700 nm against the methanol blank. Ferric reducing power (iron (II)) was quantitatively derived using the following equation: and C � c × V/m, where C is the Fe (II) content of the FRAP solution in the plant extract (RE/g), c is the concentration of standard obtained from the standard curve (mg/mL), V is the volume of plant extract used (mL), and m is the mass of extract used (g).

Statistical
Analysis. Data obtained from this study were expressed as mean ± standard deviation, based on triplicate experimentation. e statistical analysis carried out was done using the one-way analysis of variance (ANOVA) and mean separation by Fischer's LSD on the MINITAB statistical package.  For M. paradisiaca: gallic acid > rutin > plantain boiled peel extract > plantain peel aqueous extract > plantain peel acetone extract > plantain flesh ethanol extract > plantain peel ethanol extract > plantain flesh acetone extract > plantain flesh aqueous extract at the highest concentration (0.01 mg/mL). Table 3 shows that the standard drugs (gallic acid (84.03 ± 0.002%) and rutin (64.12 ± 0.01%)) and all aqueous and ethanol extracts of the flesh and peel in M. sinensis and M. paradisiaca had peak antioxidant activity at the highest 4 e Scientific World Journal concentration gradient (0.01 mg/mL). A similar pattern existed in the acetone extracts of M. sinensis flesh (3.93 ± 0.02%) and peel (11.17 ± 0.002%) and M. paradisiaca peel (11.01 ± 0.002%). Table 4

ABTS.
e ABTS radical scavenging activity of extracts shows that gallic acid had a generally higher scavenging activity than the rutin. e percentage ABTS inhibitory activity of the banana flesh and peel and peel extracts as well as the standards was of the following order: rutin > banana boiled peel extract > banana peel aqueous extract > banana peel ethanol > banana peel acetone extract > banana flesh acetone extract > banana flesh ethanol extract > banana flesh aqueous extract at the highest concentration (0.08 mg/L).
On the other hand, in plantain, the ABTS inhibitory activity order was rutin > plantain boiled peel extract > plantain peel aqueous extract > plantain peel ethanol  e Scientific World Journal 5 extract > plantain peel acetone extract > plantain flesh ethanol extract > plantain flesh acetone extract > plantain flesh aqueous extract at the highest concentration level (0.08 mg/mL). Table 5 shows that the rutin standard (99.10 ± 0.0004%) and acetone and ethanol extracts of M. sinensis and M. paradisiaca flesh and peel had the peak ABTS radical scavenging activity at the highest concentration gradient (0.08 mg/mL). However, amongst the aqueous extracts, all except M. sinensis and M. paradisiaca flesh had peak activity at the highest concentration gradient. M. sinensis flesh had its peak activity at 0.04 mg/mL, while M. paradisiaca had its own peak activity at 0.01 mg/mL concentration gradient. Table 6 shows that peak activity in the plant extracts was observed in the acetone extract of M. paradisiaca flesh (<0.0025 mg/mL). However, ethanol and aqueous extracts of the flesh and peel, including the peel extract of M. sinensis and M. paradisiaca, had IC 50 values above the highest concentration (0.01 mg/mL). From the IC 50 , ABTS had its peak activity in the peel extract of M. sinensis (0.03 mg/mL), followed by the aqueous extract of M. sinensis peel (0.04 mg/ mL). On the other hand, M. paradisiaca had a joint peak activity in aqueous extracts of the peel and peel extract (0.04 mg/mL) ( Table 6).
e negative values observed in acetone and aqueous extracts of M. paradisiaca flesh (DPPH and ABTS) could suggest the preoxidant activity at particular concentration levels and potent scavenging capacity as well.

Correlation between Phytochemical Constituents and Antioxidant Activity of M. sinensis and M. paradisiaca Flesh and Peel Compartments
Pearson's coefficient was used to analyse the relationship existing between the pharmacological variables (total phenol, proanthocyanidin, and flavonoid) and antioxidant capacity (DPPH, ABTS, and FRAP) of the flesh and peel compartments of M. sinensis and M. paradisiaca fruits. Table 7 shows a negative correlation between the respective phenolic, proanthocyanidin, flavonoid constituents, and the IC 50 Table 7).
ere was negative correlation between the phenolic, proanthocyanidin, and flavonoid constituents and the IC 50 Table 7).
Medicinal plants are widely used for their therapeutic potential which they derive from their array of bioactive principles.
ey are key in frontiers of natural products, green chemistry, and drug development [29].  Phenolics are secondary metabolites and are broadly dispersed in plant tissues. ey are responsible for pigmentation, taste, and flavour in fruits [30]. A large number of phenols such as eugenol are responsible for taste [31]. Phenolic compounds have antioxidant activity which has stimulated nutritional interest [32]. e phenol content was high in all acetone and ethanol extracts of M. sinensis and M. paradisiaca which is similar to the phenolic levels detected in a number of medicinal plants such as Terminalia arjuna, T. bellerica, T. chebula, and Phyllanthus emblica [33]. Likewise, the observation is in line with the high phenolic content observed in Morus nigra cv. Cherokee, Solanum melongena cv. Blacknite, Vaccinium corymbosum cv. O'Neal, and Capsicum annum as reported by [34] just as [35] also reported similar phenolic content outcomes in the mesocarp and pericarp of Mauritia flexuosa and eobroma bicolor.
Proanthocyanidins, also termed condensed tannins, are polymers of flavonoid molecules [36] present in fruits and flowers of several plants which function as a defence against biotic and abiotic stress and possess antitumor, antioxidant, and immunostimulatory capacity [37]. Furthermore, proanthocyanidins are hypoglycaemic agents reported to have free radical scavenging capacity [38]. ey also have inhibitory activity against cancerous cells in the liver [39] and ovarian section [40]. Proanthocyanidin content is high in the ethanol extracts of M. sinensis and M. paradisiaca peels and is comparable to that of similar solvent extractants of Brachylaena ilicifolia [41]. In addition, the high proanthocyanidin contents in the acetone extracts of M. sinensis and M. paradisiaca fruits are similar to those detected in Cucumis africanus fruit by [42]. However, the low levels in the aqueous extracts are similarly corroborated by qualitative profiles of aqueous extracts of M. sapientum and M. paradisiaca and ripened Saba banana as well as in bracts of the Karpuravalli, Nendrum, and Poovan cultivars of banana [43][44][45].
Flavonoids are major secondary metabolites with extensive distribution in plants and pharmacological potentials [46]. ey are non-nitrogenous compounds responsible for pigmentation in fruits, leaves, and petals [47], which are majorly sourced from fruits, vegetables, and cereals [48].
Flavonoids, known for their interaction with neurological signalling pathways [49], have therapeutic effects in neurodegenerative disease conditions. Dietary flavonoids have biochemical anticancer mechanisms by making the aryl hydrocarbon receptor (AhR) the target site [50] and similarly in Alzheimer's disease (AD) and atherosclerosis [51,52] and chemoprevention and disease therapy [53]. ey are also key in reducing the risk of chronic conditions and the improvement of blood pressure [54]. Notably, [55] has elucidated the use of dietary flavonoids in alternative medicine in relation to the control and management of epileptic conditions. Flavonoid content was high in the acetone and ethanol extracts of M. sinensis and M. paradisiaca flesh and peel, including the boiled peel extract in both fruits, but was the highest in the acetone extract of each of the flesh and peel of both fruits, a similar observation in ethyl acetate extracts of the muli cultivar of banana [56].
ere was undetected to fairly low flavonoid content in the aqueous extracts of M. sinensis and M. paradisiaca in this study. However, the flavonoid content in M. paradisiaca boiled peel extract in this study is comparable to the observation in the aqueous extract of Rubus niveus fruit [57] and is also comparable to similar pharmacological profiles such as antiproliferative, antioxidant, and enzyme inhibitory activities of Rubus caesius L. [58,59]. Generally, the higher phytochemical contents detected in the acetone and ethanol extracts over the aqueous extracts of M. sinensis and M. paradisiaca fruits suggest the superior extractive potency of organic solvents over the somewhat weaker potency in aqueous solvents [60]. is observation, particularly in acetone solvents, is not far-fetched due to the nature of the solvent's polarity, with extensive significance on the components and activity of bioactive compounds [61]. Furthermore, [62] has buttressed the point about organic solvents' high extraction efficiency.
Antioxidants are compounds that neutralise free radicals and can be supplied in diets. Dietary antioxidants have key pharmacologically important roles such as male reproductive health improvement [63], insulin resistance, and lowered risk of type II diabetes [64]. Essentially, antioxidants facilitate regular body functions such as normal cell growth, immune support, and prevention of degeneration at molecular levels, including the stemming of premature ageing [65]. Furthermore, research workers have mirrored areas such as the in vitro evaluation of the biological activities of unripe M. paradisiaca against the oxidative and cell-damaging phenomenon of lipid peroxidation in the pancreatic organ of rats [66]. Antioxidant potential was evaluated with the DPPH, ABTS, and FRAP assays, in order to account for the variances present in the mode of activity of antioxidants. ere was a generally low DPPH activity in the acetone, ethanol, and aqueous extracts of the flesh and peel, as well as the boiled peel extracts in M. sinensis and M. paradisiaca.
is could be accounted for due to the reported direct relation between DPPH radical scavenging activity and proanthocyanidin content [67]. Inhibition of ABTS + (%) by the extracts was generally higher than in DPPH, alluding to a lower IC 50 value than DPPH, which is explainable by the variations in the mechanism in the two radical antioxidant reaction assays. e organic extracts (acetone and ethanol) predominantly had the highest DPPH scavenging activity in the peel, flesh, and boiled peel extract of M. sinensis. is observation can be attributed to the implication of phenolic content in the antioxidative mechanism [68]. e synergistic relationship between phenolic compounds and antioxidant activity in plants has also been elucidated [69][70][71]. Reference [72] similarly observed good antioxidant scavenging activity in the peel of Solanum tuberosum, Zingiber officinale, Ipomoea batatas, and Raphanus sativus. ABTS inhibition was the highest in aqueous extracts of M. sinensis and M. paradisiaca peels and was even higher in the boiled peel extracts of M. sinensis and M. paradisiaca. e high phenolic content can be reflective of the observed antioxidant activity, especially because phenols and especially flavonoids are bioactivity precursors [73][74][75] and key contributory factors to the antioxidative potential of plants [76,77]. e FRAP assay is a well-known, reliable approach that measures ferric reducing ability in a redox-linked colorimetric reaction that reduces Fe 3+ to Fe 2+ [78][79][80][81].
e reflectively high FRAP activity on the basis of IC 50 [82]. e positive correlation between phenolic content and the ABTS activity in M. sinensis peel, M. paradisiaca flesh and peel, FRAP activity of M. sinensis peel, and M. paradisiaca flesh is in conformity with reports of phenolic compounds being related to antioxidant activity [83][84][85]. However, the general positive and negative correlational patterns indicate an unpredictable correlation between the two pharmacological factors and suggest that none of the polyphenolic constituents contributed exclusively to antioxidant activity, but instead that a combination of the polyphenolic constituents contributed to antioxidant activity [71,86].

Conclusion
In a bid to further enhance the utility of the phytomedicinal potential of M. sinensis and M. paradisiaca fruits, this study has shown their therapeutic potentials on the basis of their polyphenolic constitution and richness, chief of which are flavonoids and phenol in the compartments (flesh and peel) of the fruits. e bioactivity and therapeutic potential of the plant extracts are also largely inclined to the organic-based extraction solvents, with the acetone extracts being dominantly bioactive and thus suggested to be the most pharmacologically preferred extraction solvent. e biological activity reflects the viability of M. sinensis and M. paradisiaca fruits as useful natural antioxidants, against oxidative stress which improves and buttresses the nutritional acceptability and medicinal capacities, respectively, of the flesh and peel components of these fruits. Furthermore, M. sinensis and M. paradisiaca flesh and peel should be incorporated into the human diet due to comparable ferric reducing antioxidant capacity with standard drugs such as rutin.
ere also portend higher therapeutic benefits upon the combined consumption of the three fruit components.

Data Availability
e data used to support the findings of this study are available upon request to the authors.

Conflicts of Interest
e authors declare no conflicts of interest.