Phytochemical Profile and Antioxidant Activity of Nigella sativa L Growing in Morocco

Background Nigella sativa L (NS) is a powerful antioxidant and medicinal plant with many therapeutic applications particularly in traditional medicine for respiratory, gastrointestinal, rheumatic, and inflammatory disorders, as well as cancer. Objective The aim of this study is to extract the active ingredients from the Moroccan Nigella sativa L and determine its antioxidant properties. We hypothesize that the separation of the compounds from Nigella sativa L has either a positive or negative effect on antioxidants. To study this, we explored different methods to simultaneously extract and separate compounds from Nigella sativa L and performed antioxidant tests (β-carotene and DPPH) for all collected fractions. Methods Nigella sativa L was hot-extracted by Soxhlet and mother extracts and was separated using silica column chromatography with adequate eluents. Qualitative phytochemical tests to determine the chemical families in Nigella sativa L seeds were performed on the fractions. They were also identified and characterized by GC-MS and HPLC-DAD. Then, antioxidant activity was examined by β-carotene bleaching and DPPH radical scavenger tests. Results and Conclusion. The mother extract hexane FH generated eight different fractions (SH1-8) and the acetone extract FA generated 11 fractions (SA1-11). The FH fractions had a high percentage of fatty acids, and the FA fractions had some interesting polyphenols derivative compounds. Phytochemical screening revealed secondary metabolites such as polyphenols flavonoids, alkaloids, steroids, terpenes coumarins, tannins, and saponins. We found that only two solvents (hexane, acetone) of different polarities could easily extract and simultaneously separate the components of Nigella sativa L. The antioxidant fractions that we collected had close activity to reference compounds but were more active than the corresponding mother extracts. Moreover, several IC50 values of fractions from acetone extract were better than those from hexane. Therefore, the antioxidant activity of Nigella sativa L is more attributed to flavonoids and polyphenols than fatty acids. In summary, the separation of hexane extract presents a more pronounced positive effect for antioxidant tests than acetone extract.


Introduction
Artificial antioxidants such as butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA) are commonly reported for their efficiency in delaying cell deterioration but are also suspected to have negative health effects like carcinogenesis and toxicity [1]. erefore, replacement with natural antioxidants could reduce health risks. Nigella sativa (NS) is a traditional and natural source of antioxidants [2,3]. In fact, NS is capable of free radical inhibition [4,5] and can also significantly reduce oxidative stress [6,7].
Nigella sativa, or black caraway, is a medicinal plant with many therapeutic uses in traditional Jordanian folklore. It is used to treat respiratory, gastrointestinal, rheumatic, and inflammatory disorders, as well as cancer [8,9]. It is also used to treat various respiratory disorders across the globe in Morocco, Pakistan, and Southern Europe [10]. e seeds of NS are used as a spice in bread, yogurt, marinades, sauces, and salads. In Islamic culture, NS is known as "El Habba Saouda" and is used in traditional medicine with reference to a proverb stating it is "a drug for all diseases except death." ese words remained a mystery, until science was able to determine its therapeutic properties [11].
Considering the richness of its biological heritage, it is possible that the seed extracts could contain one or more active ingredients that specifically target each disease. Indeed, the diversity of secondary metabolites [12][13][14] can explain this variety of therapeutic uses.
Most previous studies explored the compounds hot-or cold-extracted with polar or nonpolar solvents as well as the essential oil [15,16]. ese extracts contain a mixture of several families of chemical compounds; therefore it is highly unlikely that a single well-defined compound explains all therapeutic uses. In this work, we focus on two essential mother extracts that may contain distinct groups of secondary metabolites.
Hexane is a nonpolar solvent, which can only extract nonpolar compounds such as lipids (fatty acids). On the other hand, acetone is a polar solvent that can extract polar entities such as flavonoids and polyphenols. e use of a solvent of intermediate polarity (chloroform or ethyl acetate) that contains a mixture of the two groups of compounds is likely to inhibit separation.
is study aimed to extract and separate these secondary metabolites simultaneously and evaluate their antioxidant activity. To the best of our knowledge, this is the first study to use chromatography to separate secondary metabolites of Moroccan Nigella sativa. To do this, we separated the mother extract, with known antioxidant activity, and generated several fractions, which in turn pass the antioxidant tests. Only those fractions exhibiting antioxidant activity moved forward with testing.
Migration of the compounds on TLC plates was used to determine the number of compounds, or groups of compounds, existing in the fraction, and was observed directly on the plates using a UV lamp. e GC-SM and HPLC-DAD analysis was used for compound identification and to explain the antioxidant activity of each fraction.

Plant Materials.
e Nigella sativa seeds L used were harvested in Morocco during the last season and purchased from a local market at Oujda city. ey were cleaned and ground in a blender to obtain a fine powder and stored in a dark place for future use.

Extraction.
e powder was subjected to extraction using the following solvents: hexane, chloroform, ethyl acetate, and acetone. e extractions took place over 24 hours using a Soxhlet apparatus at 40-50°C. e solvent was then evaporated on a rotavapor (BUCHI Rotavapor R-210) in vacuum rotary at 40°C. e percentage of the extract yield was calculated using the mass of the seeds powder initially placed in the Soxhlet.

Fraction's Separation by Column Chromatography.
Only two extracts were chosen for evaluation, those of hexane and acetone origin, due to the time-sensitive nature of separation by column chromatography. ese two solvents were chosen because for their significant lipophilia gap; the difference of polarity would allow for simultaneous extraction and separation of compound groups with very varied physicochemical properties. In fact, hexane is a nonpolar solvent which promotes the solubility of hydrophobic compounds, while the polar solvent acetone solubilizes hydrophilic compounds.

Gas Chromatography Coupled to Mass Spectrometry
(GC-MS) Analysis. Fraction investigation was performed on GC-MS using SHIMADZU instrument (GC-MS-QP2010) under computer control at 70 eV. About 1 μl of every fraction was injected into the (GC) column (30 m × 0.25 mm, 0.25 μm) under a flow rate of helium gas equal to 1.4 mL/ min. Scanning continued for 28 min and the ionization temperature was maintained at 200°C. e identification and quantification of the compounds were determined by comparing retention indices and spectral mass fragments with computer library NIST147 LIB [17].

High Performance Liquid Chromatography Coupled with Diode Array Detector (HPLC-DAD) Analysis.
e identification of phenolic compounds in the acetone extract fractions was carried out on an analytical HPLC using Waters e2695 and diode array detector. Chromatographic separation was performed through a C18 column (5 μm, 250 × 4.6 mm). e solvents composition and the gradient elution process followed Mechraoui et al. [18] with some modifications. e elution was performed by a binary gradient system (A: water/ acetic acid (2%v/v) and B: acetonitrile, pH � 2.6). e gradient process was 0-5 min: 95% A and 5% B, then 25-30 min: 65% A and 35% B, 35-40 min: 30% A and 70% B; and 40-45 min: 95% A and 5% B. A flow rate of 0.9 mL/min was used and 30 μL fraction samples were injected after being passed through a 0.45 μm filter. e UV detection was between 280 and 360 nm [18]. Phenolic compounds were identified by comparing retention time and UV-detection relative to the standards.  [19,20]. Phytochemical screening was performed with thin layer chromatography. TLC plates of silica gel supported by aluminum plates (fluorescent index 254 nm Fluka Analytical, Sigma-Aldrich) and eluents were used to achieve the separation. Different solvent developers were used with the reagents for the chemical screening.
Steroids/terpenes. Sterols were identified by Liebermann-Burchard reagent. 50 mL of ethanol was added to 5 mL of acetic anhydride and 5 mL of sulfuric acid on ice. e fraction characterization was performed using TLC plates pulverized at 100°C for 10 min. [19].
Alkaloids. Mayer reagent (1 g of plant extract was placed with potassium mercury tetraiodide and water in a test tube) was used for the extraction of alkaloids. Precipitation would occur if the test was positive. Dragendorff reagent: potassium tetraiodobismuthate was pulverized on a TLC plate for fraction separation. Orange spots were detected by UV lamp at 365 nm [21].

Flavonoids.
A solution of AlCl 3 and 1% ammonium was pulverized on a TLC plate with the fraction. Yellow spots indicated flavones, blue spots showed phenolic acid, and red spots revealed the existence of Gallic acid [22].
Saponins. 2% SbCl 3 in chloroform solution was pulverized on a TLC plate with the fraction. Red fluorescent spots were detected at 254 nm by a UV lamp [20].
Polyphenols. Polyphenols were identified by Folin-Ciocâlteu reagent. On a TLC plate with the fraction, Folin-Ciocâlteu reagent was pulverized, and the spots were detected at 365 nm by a UV lamp.

Tannins.
A solution of 2% FeCl 3 and acetic acid was pulverized on the fractions and placed on a TLC plate. Yellow spots were detected at 366 nm [21].
Coumarins. Coumarins were identified by two processes. First, a solution of KOH 2% was pulverized on a TLC plate or by a pulverized solution of 5% (CH 3 CO 2 )Pb on MeOH. e spots were visualized at 365 nm by a UV lamp [21].

β-Carotene Bleaching Test.
is method was developed by Sun and Ho [23] and is based on the measure of absorbance at 470 nm due to the decomposition of linoleic acid. An emulsion of β−carotene-linoleic acid is prepared with Tween20 and hydrogen peroxide. 2 mg β-carotene is dissolved in 10 mL chloroform. 1 mL of this solution is added to 20 μL linoleic acid and 200 mg Tween 40. e chloroform is evaporated at 40°C under vacuum using a rotary evaporator and the residue is taken up to 100 mL by aerated distillated water with vigorous agitation. 4 mL of the emulsion is placed in a series of tubes containing 200 μL samples of the fractions at different concentrations. e tubes are well shaken, and the absorbance is read at once at 470 nm. e tubes are placed at 50°C for 120 minutes with a control, which contained only the emulsion and methanol. Absorbance was recorded and the inhibition percentage was expressed using equation (1). IC 50 is the 50% inhibition concentration determined graphically on the % I � f (C) curve by the extrapolation on the X-axis for the value of Y � 50% inhibition. IC 50 was determined for the extracts and fractions. For β-carotene, the inhibition percentage was calculated according to the following equation: where AA (120) is the absorbance at 120 min; AC (120) is the control absorbance at 120 min; and AC (0) is the control absorbance at 0 min.

DPPH Radical Scavenging
Activity. DPPH radical activity was carried out by the Gramza-Michalowska method [24]. DPPH is a stable radical, which has a free electron on the nitrogen atom. In the presence of antioxidant DPPH, the color changes from violet to yellow [25]. In this test, various concentrations of fractions were added to the methanol solution of DPPH radicals (0.004% m/v). e samples were incubated for 30 minutes at room temperature and the absorbance was recorded at 517 nm against a blank solution. e percent inhibition was calculated using equation (2). e inhibitory concentration (IC 50 ) is determined as the concentration that inhibit 50% of DPPH (I% � 50). e inhibition percentage is calculated using the formula below: where Ac is the absorbance of DPPH without antioxidant (negative control) and Asample is the absorbance of DPPH in the presence of an antioxidant.

Extraction's Yield.
In the successive extraction of the Nigella sativa L seeds (NS), the yields of mother extracts (FH and FA) were calculated relative to the mass of the starting powdered seeds placed in the Soxhlet apparatus. Hot extraction is more beneficial than cold extraction because the maceration and provides significantly higher yields [26]. In general, the color of the mother extracts obtained is more or less deep brown. e results show that the mother hexane extract, FH, is the most abundant (

Hexane and Acetone Extracts
Separation. Only two promising mother extracts from hexane FH and acetone FA were separated by silica gel chromatography. Separation was slow and fractions were collected every five min and systematically assessed on TLC plates. Table 1 shows the yield of assembled fractions in relation to their respective mother extract. e yields were calculated relative to the mass of the mother extracts FH and FA used at the beginning. Following this procedure, the hexane mother extract generated eight fractions, SH1-8, and that for acetone fractions there were 11 fractions, SA1-11. e eight fractions generated by hexane (Table 1) show varying yields with the highest being SH1 with 72.35% followed by SH7 (9.11%). e others give yields less than 5.12%. e yields of fractions generated by acetone extract were calculated relative to the mass of the mother extract of acetone (FA) introduced at the beginning. SA1 represents the highest yield at 62.88% followed by SA11 at 15.82%. e other fractions were all less than 10%.

Hexane Extract.
e results of FH characterization by GC-MS are presented in Figure 1 and Table 2. e recorded chromatogram after the extraction (Figure 1) shows the eminent presence of eight compounds. In Table 2, the chemical composition of 85.9% is identified as linoleic, palmitic, Hexadecanoic, and oleic acids.
We noted that, according to Table 2, the seeds from eastern Morocco mainly contain linoleic acid (80.26%) and much less palmitic and oleic acid. is is in line with the work of several other authors [10,27,[29][30][31]. e presence of other compounds was less than 6.24%, but it should be noted that the majority of the compounds extracted had similar structures. It was also observed that the rest of the compounds extracted differed in the literature. is can be explained by spontaneous chemical reactions occurring as a result of physical conditions (i.e., light and temperature) without any experimental intervention.
In Morocco, Gharby et al. [29] reported 58.5% of oleic acid after extraction of esterified hexane extract, while Khodami et al. [27] showed that hexane extract of NS was mainly unsaturated fatty acids such as palmitic, linoleic, and oleic acids. Piras et al. [30] reported that Turkish NS contained 55% unsaturated fatty acids. Unidentified components in the esterified hexane extract represented only 13%.
ose might be dependent on the esterification/methylation process. Ekowati et al. [31] and De Oliveira and Khater [10] reported many other compounds not found in our work.

Fractions of Hexane Extract.
e identification results of fractions (SH1-8) from FH by GC-MS analysis are presented in Table 3. e results indicate the presence of a considerable variety of compounds. We observed that the majority were fatty acids.
Several constituents were present in different fractions (e.g., palmitic acid identified in fractions SH1, SH2, SH3, SH5, SH6, SH7, and SH8; linoleic acid in SH1, SH2, SH5, and SH7 and oleic acid found in fractions SH3, SH6, and SH8. e appearance of fatty acids in many fractions could be explained by the disturbance of compounds migrating in the column because of the abundance of fatty acids. In fact, a high quantity of fatty acids and their derivatives migrated poorly in the column chromatography. To overcome this problem, we proceeded to centrifugation and then cooled the extract to −4°C to separate the solid fat. Only 1.5% of the fatty acids were eliminated using this method.
In general, naturally extracted plant products are continuously transformed by oxidation or other chemicals reactions. Consequently, their percentage varies indefinitely despite good conservation. For example, olive oil retains its physicochemical characteristics for a whole year, but loses taste quality over time [32]. e variation of the quantity and quality of the hexane extract and fractions during migration in the chromatography column is justified. In addition, the chromatography column is slightly acidic, and the eluting solvents contain traces of transition metals, which can cause additional chemical reactions and transformations.

HPLC-DAD Analysis.
e results of the HPLC analysis are presented in chromatograms (Figure 2) that show the constituents of the acetone extract and its fractions, while     Table 4 shows the identified constituents. On the Y-axis, the values of the peak's intensities are arbitrary. We can compare the intensities of the peaks on the same scheme, but we cannot compare them with the others because the collected fractions were concentrated each time by evaporation in a rotavapor. erefore, we can only compare the position of the peaks in the chromatogram on the X-axis. e acetone extract fractions gave different chromatograms compared to the hexane fractions. e peaks showed varying intensity depending on their abundance (Figure 2). e FA contained a mixture of several compounds. Gallic acid is the major compound in Figure 2. It is also clearly visible in the SA1, SA2, and SA3 fractions. Our results are in agreement with those reported by Mechraoui et al. [18]. Except the kaempferol, all compounds found in the mother extract disappeared in the separated fractions. It should be noted that the separation was successful since, at the end, each fraction contained only one (SA1, SA4), two (SA3, SA7), or three (SA2, SA11) compounds. Due to technical problems, some fractions (SA5, SA6, and SA8) could not be analyzed and identified. Tables 5 and 6 summarize the results of the phytochemical screening for all hexane and acetone extract and fractions. e presence of phytochemical components of steroids, terpenes, coumarins, alkaloids, polyphenols, flavonoids, and saponins was determined. e hexane mother extract had steroids, terpenes, tannins, saponins, and alkaloids (Table 5). However, coumarins, polyphenols, and flavonoids were absent. e acetone mother extract only contained polyphenols, steroids, tannins, flavonoids, and alkaloids (Table 6), while coumarins and saponins are not present.

Phytochemical screening.
Most fractions had alkaloids and saponins as well as several secondary metabolites like steroids and terpenes. Coumarins, polyphenols, and flavonoids appeared in some fractions despite their absence in the original extract (Table 5). is can be explained either by the phenomenon of opacity, where certain colors can be concealed by others, or by disparity of the color's wavelengths during measurements.
e phytochemical results of the NS hexane extract are similar to those reported by Javed [33], except in the absence of sterols in their hexane extract. Other phytochemical investigation [34] described close phytochemical screening to our results. Nevertheless, coumarins are reported and saponins were absent in our results.
Contrary to the hexane mother extract, the compound families in the acetone mother fraction were the same as those in the separation fractions (Table 6). e presence of polyphenols and flavonoids is marked in several fractions, and less for other chemical families. In contrast, some authors report that acetone extract has a high level of steroids and are low on coumarins [34]. Table 7 presents the values of the inhibitory concentrations necessary to reduce 50% oxidation (IC 50 ) by the antioxidant β-carotene. e values are given for both hexane and acetone mother extracts, as well as their corresponding fractions, which are compared with the reference value of butylhydroxyanisole (BHA). At the beginning, the quantity collected from certain fractions was extremely low. Hence, we could not perform totality tests of all fractions without exhausting our stock. All hexane fractions were more active than their mother extract FH; however the acetone mother extract FA was more active than three of its fractions. e activity of fractions from the hexane extract was dispersed as follows: SH4 > SH1 > SH7 > FH. SH4 had the highest activity and resemblance to the reference BHA (Table 7), likely due to the presence of steroids and terpenes. Although SH1 and SH7 also contained steroids and flavonoids, they had a weak activity compared to SH4. is can be attributed to the presence of other families of chemical compounds, which inhibit the apparent activity. e characterization by GC-MS showed the existence of many antioxidant components [35], which have similar structure to BHA. In addition, derived terpenes were identified, such as 2.4-decadienal and (Z) 9-Tricosene. Moreover, fatty acid analogues, like linoleic acid, 17 pentatriacontene, or 2.4-decadienal, were also found. To support this claim, SH4, SH1, and SH7 were compared to vegetal species with high antioxidant activity like sage, thyme, and curcuma [35,36]. In the extract's lipids, the present antioxidant and anticancer phytosterols were also detected [37,38].

β-Carotene Bleaching Test.
In acetone extract and fractions, we observed that the inhibition activity is dispersed as follows: e first group of fractions composed of SA5 and SA10 had a much higher activity than FA with IC 50 remarkably close to BHA ( Table 7). e second group composed of SA2, SA1, and SA4 exhibited weaker antioxidant activities than FA.
Singh et al. [39] reported that FA was the most interesting part of NS because it has the highest antioxidant       [41]. e only pure flavonoids like apigenin, quercetin, and naringenin gave interesting antioxidant activity [42,43]. is result can be extrapolated to the results found for fractions SA4, SA5, and SA10 since they only contain these flavonoids.

DPPH Radical Scavenging
Activity. e values of (IC 50 ) determined by the DPPH antioxidant tests are summarized in Table 8. ey were carried out for the hexane and acetone mother extracts, as well as their corresponding fractions, which were all compared with the reference value of ascorbic acid. IC 50 of the hexane extract was the highest and the acetone mother extract was significantly lower than four of its fractions.
In the case of hexane, the SH7 and SH8 were more active than FH, particularly SH8 whose IC 50 was three times less. e antioxidant activity increases as follows: SH8 > SH7 > FH.
In the case of acetone, the activity value of the mother extract was intermediate between all fractions; the antioxidant activity increases in the direction e three most active fractions, SA11, SA5, and SA10, all had an IC 50 lower than FA but not comparable to the reference ascorbic acid.
Considering the two antioxidant tests, β-carotene, and DPPH • , the fractions from the acetone extract were more active than those from the hexane extract. For β-carotene, the best value was found in the acetone fraction SA5 with IC 50 � 0.064 mg/mL, which is remarkably close to the BHA reference, which has a value of IC 50 � 0.053 mg/mL. In the case of DPPH, all the acetone fractions showed acceptable IC 50 values, particularly fractions SA5, SA10, and SA11 which had mean IC 50 values around 0.25 mg/mL. However, they are extremely far from the reference ascorbic acid (0.018 mg/mL).
Haroun [15] reported that NS hexane extract from two different places in the same country (Malaysia) gave IC 50 � 8.17 mg/mL for NS harvested in Shah Alam and IC 50 � 4.48 mg/mL for NS harvested in Kentalan. In Yemen, DPPH test showed an IC 50 � 12.79 mg/mL, in Sudan it was equal to 4.48 mg/mL [15], and in Ethiopia IC 50 was 8.52 mg/ mL. Soulaimanifar et al. [15] reported a very high IC 50 value, 104.76 mg/mL, in Iran. In Tunisia, Ksouda et al. [44] reported that NS hexane extract had IC 50 � 31 mg/mL, a very close value to our 23.25 mg/mL. e origin of the seeds and the extraction method are key factors for the quality and quantity of secondary metabolites. In Malaysia, the DPPH test on hexane extract by supercritical fluid extraction gave an IC 50 � 1.58 mg/mL [45].
SH7 antioxidant activity could be explained by its phytochemical analysis due to the presence of terpenes, steroids, polyphenols, and flavonoids in the fraction. Furthermore, GC-MS analysis gave diene functional groups and unsaturated fatty acids, which are known by their antioxidant activity. Palmitic acid is reported to have an antioxidant effect [46] on β−carotene test [47,48]; polyphenols derivatives like benzoic acid can also inhibit oxidation [47,49]. SH7 and SH8 showed similar chemical compositions with fatty acids, and this may explain a part of their antioxidant activities.
e antioxidant activity of FA could be attributed to its flavonoids. HPLC-DAD identified gallic acid, hydroquinone, apigenin, naringenin, ascorbic acid, cysteine, rutin, quercetin, and kaempferol. Mechraoui et al. [18] reported an IC 50 � 0.1602 mg/mL from Tunisian NS. IC 50 was 2.69 mg/ mL from a Pakistani NS. ese results are different from ours with an IC 50 equal to 0.79 mg/mL. e chemical structure of the fractions was identified by HPLC-DAD and the fraction's antioxidant activity was compared to commercial pure compounds. Pure commercial gallic acid IC 50 was reported as 1.50 µg/mL [40], apigenin IC 50 was 30.3 µg/mL, rutin had an IC 50 � 23.7-50 µg/ mL [41,50], and quercetin presented with an IC 50 � 1 µg/mL [40]. ese products are the essential compounds in fractions SA1, SA2, and SA11. It is clear that fractions of a single compound and fractions with multiple compounds do not have the same antioxidant activity level.
ere can be a positive synergistic effect on the activity or a negative one (inhibitor effect). If we compare the activity of SA1 and SA3, we can see that catechol in the presence of gallic acid has a  negative effect on the antioxidant activity, whereas, in the case of SA2, hydroquinone and apigenin had a positive effect on activity. erefore, testing is required to be able to predict the synergistic effects.

Conclusion
is study describes the simultaneous process of extractionseparation of chemical components of Nigella sativa L seeds based on the polarity of two well-chosen solvents. We have succeeded in extracting and separating two families of compounds with different physicochemical and phytochemical properties. Analysis and separation methods like GC-MS and HPLC-DAD allowed us to characterize and identify the majority of the mother extracts and separated fraction constituents. It should be noted that the Moroccan Nigella sativa L is rich in linoleic acid but low on oleic or palmitic acid.
Antioxidant activity was evaluated by β-carotene bleaching and DPPH scavenger. Antioxidant activity was attributed to each fraction and explained by its constituents. Nigella sativa L contains important secondary metabolites and is as a reliable source of antioxidant compounds. Moroccan NS gives a competitive IC 50 compared to several countries around the world.
We show that secondary metabolite separation is beneficial for antioxidant activity. In fact, we repeatedly found that the fractions were more active than the mother extracts. In addition, some of the fractions had an underlined activity compared to the references.
We have succeeded to identify some of the interesting fractions generated by the mother extract and specified the compounds responsible for antioxidant activities. In fact, it can be said that, for β-carotene test, antioxidants are concentrated in hexane fractions SH4, SA5, and SA10. For DPPH test, fractions SH8, SA5, SA10, and SA11 include the active entities of Nigella sativa L. Future research should explore the active fractions in vitro and compare their antioxidant activity in animal models before and after induction of diseases caused by oxidative stress.

NS:
Nigella sativa FH: Hexane extract FA: Acetone extract SH1-8: Fractions separated from hexane extract SA1-11: Fractions separated from acetone extract GC-MS: Gas chromatography coupled to mass spectroscopy HPLC-DAD: High performance liquid chromatography coupled to diode array detector.

Data Availability
e data used to support the study are included within the article and are also available from the corresponding author.

Conflicts of Interest
e authors declare that they have no conflicts of interest.