Protective Effect of Red Okra (Abelmoschus esculentus (L.) Moench) Pods against Sodium Nitrite-Induced Liver Injury in Mice

Vegetables, drinking water, and preserved meats may contain sodium nitrite (NaNO2), which causes liver disease by inducing oxidative stress. Phytochemicals are highly recommended as an alternative to synthetic drugs and affordable medicines to treat liver disease because they have fewer or no side effects. Therefore, this study aims to determine the antioxidant and hepatoprotective potential of red okra fruit ethanol extract against NaNO2-induced liver damage. Thirty-six male mice were separated into six groups. The normal control group (WA) was given distilled water only, and the NaNO2 (SN) group was given only 50 mg/kg BW NaNO2. The other four groups (P1, P2, P3, and P4) were given NaNO2 and red okra ethanol extract at doses of 25, 50, 75, and 100 mg/kg BW, respectively. Gavage was administered orally for 21 consecutive days. Commercial kits define all biochemical parameters according to the manufacturer's instructions. Liver tissue staining followed standard protocols using hematoxylin and eosin. The study revealed that NaNO2 induction causes oxidative stress and damages the liver. The activity of antioxidant enzymes (superoxide dismutase and catalase) significantly increased in the groups treated (P2–P4) with ethanol extract of red okra (p < 0.05). Besides, the oxidants (malondialdehyde, F2-isoprostanes, and nitric oxide) in the liver homogenate significantly decreased in the P4 group, which were given red okra ethanol extract (p < 0.05). Likewise, red okra pods decreased significantly for the serum biochemical parameters of liver damage (aspartate aminotransferase, alkaline phosphatase, and alanine aminotransferase) in the P3 and P4 groups (p < 0.05). Then, it led to a restoration of the histological structure compared to exposed mice (SN), as the pathological scores decreased significantly in the P3 and P4 groups (p < 0.05), as well as the number of the necrotic and swollen liver cells was reduced. Hepatocytes returned to normal. The results showed that the ethanol extract of red okra fruit could be helpful as an affordable medicine. It is an antioxidant and hepatoprotective agent to protect the liver from damage caused by NaNO2.


Introduction
Hepatic diseases are global deadly health problems that cause human deaths. It is estimated that around 2 million deaths each year in the world is associated with liver diseases. Acute hepatitis, cirrhosis, liver cancer, and alcohol-associated liver disease are more common, causing 4% of all deaths worldwide; the percentage is expected to rise due to lifestyles with little or no physical activities and over nutrition [1,2]. Intrinsic and extrinsic factors such as being born with metabolic defects, malnutrition, viral infection, and exposure to toxic substances [3] may produce reactive nitrogen species/reactive oxygen species (RNS/ROS), facilitating liver disease illness. e liver is a vital organ responsible for the detoxification of poisonous materials such as xenobiotics. It plays various roles in metabolism, secretion of wastes, and elimination of unneeded materials from the body; hence, it is the main target of toxins and xenobiotics [4,5]. Natural and artificial manufactured food additives increase and maintain food safety, texture, taste, and nutrient value. Although they are beneficial, synthesized additives may cause some adverse effects [6]. One of the synthesized food additives is sodium nitrite (NaNO 2 ), which can be present in vegetables, cured meat, and fish. Human activities such as agriculture, which involves using nitrogenous fertilizer to increase crop productions, improper handling, and maintenance of industrial and sewage waste, increase the risk of NaNO 2 exposure to humans. Around the world, drinking water has been occasionally found to contain a level of NaNO 2 beyond the acceptable limit [7]. Nitrite has various functions, ranging from physiological, immunological to neurological functions at a low physiological amount. In the body, NaNO 2 can be interconverted into various kinds of nitrogen molecules, including nitric oxide (NO) that improves digestive health by facilitating gastric flow, preserving the integrity of the gastric epithelium and muscular barrier [8], inhibiting white blood cells from adherence to the endothelium as well as decreasing the risk of cardiovascular diseases and improving the pulmonary health. In a clinical setting, NaNO 2 is used as an antidote for cyanide poisoning [9]. In food industries, NaNO 2 is used in meat and fish as a color fixative agent to improve flavor, delay rancidity by preventing fat oxidation, and inhibit the growth of microorganisms such as Clostridium perfringens, Staphylococcus aureus, and Bacillus cereus, as well as C. botulinum that causes botulism [8,10,11].
Despite the health benefits of NaNO 2 at low concentrations, thanks to the NO that can be produced from it, howbeit the compound is detrimental at high concentrations because of its ability to oxidize various molecules such as protein, lipid, and deoxyribose nucleic acid (DNA) [5,8]. Exposure to NaNO 2 causes methemoglobinemia, hypoxia, and the formation of carcinogenic nitroso compounds due to their interaction with amide and amine in the stomach at low pH [8,12]. e nitroso compounds and other forms of nitrite generate free radicals such as superoxide (O 2 ), peroxynitrite (ONOO), and hydroxyl radicals (OH) that cause cellular damage by lipid peroxidation, protein oxidation, carbohydrate carboxylation, and DNA damage leading to cancer, congenital disabilities, dysregulation of inflammatory responses, tissue injuries, nephrotoxicity, impairment of the reproductive system, disturbance of the endocrine system, growth retardation, and hepatotoxicity [5,6,13].
Several approaches have been used for a long time to treat liver disorders, such as vaccines, corticosteroids drugs [14], and liver transplantation [4]. e approaches have some limitations, including serious side effects when used for a long time, and have limited ability to produce the desired impacts [4,14]. Alternatively, the use of natural products such as compounds from plants and their derivatives have shown promising results because they are less toxic and have little or no side effects [4,14,15].
Okra (Abelmoschus esculentus L.) Moench is a flowering plant in the Malvaceae family. It is also named ladies' finger, bamyah, bamieh, kacang, gumbo, dharos, bhindi, bendi [16], or bamia in a particular region of the world. Various parts of the red okra plant have many benefits to the body. Fresh okra heals constipation, leucorrhea, spermatorrhea, diabetes, and jaundice; the mucilage can cure diarrhea, dysentery, gastric ulcer [14,17], and syphilis [3], and when mucilage of okra is mixed with a ripe banana can be used to treat colitis, cystitis, hepatitis, and jaundice. e polysaccharides from okra modulate and improve organisms' immune response due to S. aureus infection [15]. Okra pods contain polyphenols and flavonoids such as quercetin that have higher antioxidant activity [18][19][20] can scavenge free radicals and decrease oxidative stress in the cells. e polyphenols and flavonoids can also protect the liver from the toxic effects of xenobiotics intoxication [3,14]. Specifically, red okra pods have added xenobioticsidants (anthocyanin) responsible for the pods' red color [21]. Extracts from purple okra contain anthocyanin with higher antioxidant and quercetin content than the green okra; hence, plants containing anthocyanin are more effective than the plant devoid of the compound [22]. Okra is a popular vegetable crop with good nutritional significance and specific therapeutic values, making it a potential candidate for the use of a variety of nutraceuticals and affordable medicines. e antioxidant and hepatoprotective activity of red okra pods on liver injury induced by sodium nitrite has not been reported to the best of our knowledge. erefore, this study was done to investigate the antioxidant and hepatoprotective activity of ethanol extract of red okra pods against sodium nitrite-induced liver injury in mice (Mus musculus).  [20], followed by ethanol extraction using the protocols of Yasin et al. [23]. Briefly, 5 kg of red okra pods was washed and cut into small pieces, dried without direct exposure to sunlight for seven days; after that, okra pieces were crushed with a shredder to produce a powder. e red okra powder was filtered by sieve no. 40 and stored in the desiccator. e phytochemicals were extracted using nhexane three times in 24 hours, followed by ethyl acetate (three times, 24 hours each). e residues obtained were soaked in 500 mL of pure ethanol for 24 hours three times with constant stirring. Maceration results were filtered, and the filtrates were collected and then evaporated at 60°C in a rotary evaporator to get the ethanol extract. Finally, the extracts were freeze-dried to remove the solvents and then stored at −20°C for further use. e ethanol extract was used in animal treatment from the n-hexane, ethyl acetate, and ethanol extracts since avamoney et al. [24]. e ethanol extract had higher total flavonoid, phenolic, and anthocyanin content than the n-hexane and ethyl acetate extracts.

Animals and Experimental Design.
irty-six adult male mice (M. musculus), BALB/c strain, aged 8-10 weeks, were purchased from Bratang Market, Surabaya, Indonesia, and acclimated for two weeks. Mice were housed in plastic cages (2 mice per cage) covered with wire gauze in a ventilated room with standard conditions with a 12-hour light-dark cycle in the animal laboratory, Faculty of Science Technology, Airlangga University, Indonesia. e mice were divided into six groups, each containing six mice. e groups were normal control (WA), which was given 0.2 mL of distilled water daily, NaNO 2 group (SN), which was given 0.2 mL NaNO 2 at a 50 mg/kg BW dose daily. Other groups were P1, P2, P3, and P4 that were given 0.2 mL at doses of 25, 50, 75, and 100 mg/kg BW of ethanol extract of red okra pods together with 0.2 ml of 50 mg/kg BW NaNO 2 daily. Ethanol extract from red okra was given to the mice 30 min after the administration of NaNO 2 . All treatments were given by oral gavage. During the 21 days of the treatment, the mice had free access to food and water. e Animal Care and Use Committee (ACUC) of the Faculty of Veterinary Medicine, Universitas Airlangga, approved this study (2.KE.057.04.209).

Sample Collection.
e mice were sacrificed by using ketamine 10% anesthesia. A disposable syringe collected blood from the left ventricle. e blood was left to stand in the microtube for two hours at room temperature, and after forming two phases, the liquid phase was collected. e sample obtained was stored at 4°C for further use [15]. e serum was used to determine the AST, ALT, and ALP activities, and TSP concentration. e preparation of liver homogenates followed the procedures of Cheng [25] with few modifications. e liver was cut to form small pieces then crushed with a mortar and pestle in the mixture of 5 mL phosphate buffer saline (PBS). e suspension was filtered by using a 200 μM mesh filter centrifuged at 1500 rpm for 5 min. e pellets obtained were resuspended in Tris-buffered NH 4 Cl, pH 7.2, to lyse the hepatocytes, centrifuged at 1500 rpm for 5 min. e procedure was repeated until white pellets were obtained. e pellets were sonicated at 20 kHz for 20 × 6 under ice-cold water. ey were then centrifuged at 2500 rpm for 5 min to obtain the tissue homogenates stored at 4°C. e liver homogenates were used to determine SOD, CAT, MDA, NO, and F2-Isoprostanes levels.

Determination of SOD and Catalase from the Liver
Homogenates.
e SOD activity was determined by the superoxide dismutase-(SOD-) Typed Assay kits (E. BC-K022-5) as per the kit manufacturer's instructions. e absorbances were read at wavelength 550 nm using a spectrophotometer ( ermo Scientific ™ Multiskan ™ GO), and the activity of SOD was calculated based on the manufacturer's instructions.
e CAT activity was determined by CAT (EC 1.11.1.6, K#K773-100) of BioVision as per the manufacturer's instructions. Shortly, 50 μL of the liver homogenates was put into a well plate; then, 28 μL of the assay buffer was added, followed by 12 μL mMH 2 O 2 to start the reaction. e plate was incubated at 25°C for 30 min; then, 10 μL of stop solution was added, followed by 50 μL of developer mix. After the incubation at 25°C for 10 min, the optical density (OD) values at wavelength 570 nm were read in a microplate reader ( ermo Scientific ™ Multiskan ™ GO). CAT activity was calculated as instructed by the kit manufacturer.

Determination of MDA and NO.
e MDA level was determined using Bioxytech ® MDA-586 spectrophotometric assay kit, while NO was determined by phosphoric acid, sulfanilic acid, and N-(1-naphthyl)-ethylenediamine as reported by Wahyuningsih et al. [20]. MDA and NO absorbances were measured at wavelengths 586 nm and 540 nm, respectively, using a microplate UV-Vis spectrophotometer.

Determination of F2-Isoprostane.
e concentration of F2-isoprostane in the liver was determined by Elisa kit as per the manufacturer's instructions. e absorbances were recorded at the wavelength of 450 nm with a microplate reader ( ermo Scientific ™ Multiskan ™ GO).

Determination of Liver Enzymes.
e activities of AST, ALP, and ALT were determined by ASAT (GOT) FS * (IFCC mod) 12601991021, Alkaline Phosphatase FS * (IFCC mod) 104019910021, and ALAT (GPT) FS * (IFCC mod.) 127019910021 kits, respectively, in harmony with the manufacturer's directives. e OD values were read using a UV-Vis spectrophotometer at wavelength 365 nm for both AST and ALT, and 405 nm for ALP.
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Determination of Concentration of TSP.
e concentration of TSP was determined by Pierce TM Coomassie (Bradford) Protein Assay Kit (no. 23200) as recommended by the kit manufacturer. e absorbances were read at wavelength 595 nm by a UV-Vis spectrophotometer.

Histological Examination of the Liver.
e liver histological preparation and staining followed H&E staining's standard protocols, described by Feldman and Wolfe and Salahshoor et al. [26,27]. In summary, the liver tissue was cut into two sections and fixed into buffer formalin 10% and then embedded into paraffin blocks. e liver sections in the paraffin blocks were sliced by microtome to form sections of approximately 3 μm. e staining protocols for hematoxylin and eosin were followed, then the entellan mounting medium was added before covering the slide with a cover glass. Inflammation was scored following the method reported by Giribabu et al. [28] and El-Nabarawy et al. [12] based on the number of foci recognized under the low-power field of a light microscope (100X). e inflammation grades were 0 when there was no focus of inflammation, 1 when there was one focus per low-power field of inflammation, 2 when there were two foci per low-power field of inflammation, and 3 when there was 3+ foci low-power field of inflammation. Observations were made on an Olympus 1 × 51 light microscope at the magnification of 400X to count the necrotic, normal, and swollen hepatocytes. e use of ImageJ 1.53a did the counting of the cells. All photos were taken with an Olympus DP-10 digital camera.
2.11. Data Analysis. Data were statistically analyzed using the Statistical Package for the Social Sciences (SPSS) 26.00 for windows. e data were analyzed by a one-way analysis of variance (one-way ANOVA) followed by the Duncan or Games-Howell, and the Kruskal-Wallis followed by the Mann-Whitney test. e data were presented as mean-± standard error (M ± SE), and the difference of p < 0.05 was considered statistically significant.

e Effect of Ethanol Extract from Red Okra Pods on SOD and CAT Activities.
e ethanol extract of red okra pods on SOD and CAT activities is presented in Table 1. NaNO 2 caused a significant decrease in the activity of SOD in the mice that were given NaNO 2 alone (SN) compared to the mice given water alone (WA). e ethanol extract of red okra caused a significant increase in the activity of the SOD in all red okra-treated groups compared to SN. e activity of CAT was significantly decreased in SN compared to WA. CAT activity was significantly increased in mice given red okra extract except in the group that received 25 mg/kg BW.

e Effect of Ethanol Extract from Red Okra Pods on MDA and F2-Isoprostanes.
e effects of the ethanol extract of red okra pods on MDA and F2-Isoprostanes levels in mice are presented in Table 1. NaNO 2 caused a significant increase in MDA and F2-isoprostanes in SN's liver compared to WA. e administration of ethanol extract of red okra pods ameliorated MDA and F2-isoprostanes by decreasing their liver homogenates' concentrations.

e Effect of Ethanol Extract from Red Okra Pods on NO.
e NO concentration caused a significant increase in the SN compared to WA. However, the administration of ethanol extract from red okra leads to a decrease in NO in all mice given red okra extract compared to SN.

e Effect of Ethanol Extract from Red Okra Pods on Liver Enzyme Activities.
e results of the ethanol extract of red okra pods on the activity of the liver enzymes in the serum of mice exposed to 50 mg/kg BW are presented in Table 2. e administration of NaNO 2 caused a significant increase in the ALT activity in the serum SN by two folds compared to WA. Compared to SN, the ethanol extract of red okra significantly decreased ALT activity in all groups given red okra extract except P1. Mice exposed to NaNO 2 alone showed a significant increase in AST activities compared to WA. e activity was significantly decreased in the mice administered with 75 and 100 mg/kg BW red okra extract together with NaNO 2 .

e Effect of Ethanol Extract from Red Okra Pods on the Concentration of TSP.
e ethanol extract of red okra pods on the TSP concentration in mice is presented in Table 2 NaNO 2 caused a significant decrease in the concentration of TSP in the serum compared to WA. e red okra ethanol extract administration significantly increases TSP concentration in the mice given red okra extract compared to SN.

Histopathological Examination.
e SN's histological examinations showed severe inflammation (pathological score � 3, Table 3) around the portal vein, bile duct, and around the endothelia wall of the blood capillary. In this group, many hepatocytes had undergone necrosis and swelling (Figure 1, SN). e P1 group showed severe inflammation (pathological score � 3, Table 3) around the portal vein and bile duct and inflammation at the parenchyma hepatocytes accompanied by necrosis. Furthermore, the tissue of the P1 showed swollen cells (Figure 1, P1). e P2 group showed moderate inflammation (pathological score � 2, Table 3) around the portal and the parenchyma hepatocytes. e tissues displayed necrotic and swollen cells (Figure 1, P2). e P3 group showed an improved histological structure compared to P2, P1, and SN. e tissue of P3 showed mild inflammation (pathological score � 1, Table 3) at the parenchyma hepatocytes accompanied by necrosis. e cells displayed a normal structure with few necrotic cells and swollen cells (Figure 1 P3). e P4 group showed mild parenchymal inflammation infiltration (pathological score � 1, Table 3) and few necrotic and swollen cells. Generally, the P4 group showed the tissue's normal architecture and the hepatocytes (Figure 1, P4) as the WA (Figure 1, WA). Although some liver microscopic changes were available in the SN, P1, P2, P3, and P4, the severity of the effects decreased depending on the dose of ethanol extract from red okra pods administered to the mice. e percentage of WA's necrotic cells showed the lowest percentage of necrosis, while the SN showed the highest percentage. e percentage of necrosis was significantly increased in the SN, P1, P2, P3, and P4 compared to WA. ere was no significant difference between the P1 and SN. e percentage of necrosis was significantly decreased in the P2, P3, and P4 groups than the SN and P1. e P3 and P4 groups showed a significant decrease in necrosis percentage than P2 (Figure 2(a)). e WA group displayed the highest percentage of normal cells, while the SN and P1 showed the lowest percentage. e percentage of normal hepatocytes was significantly decreased in SN, P1, P2, P3, and P4 compared to the mice who received distilled water only (WA). e administration of ethanol extract from red okra pods significantly increased the percentage of normal cells in the P2, P3, and P4 compared to the SN. Generally, all groups showed a significant difference between each other except P1 and SN (Figure 2(b)).
In summary, the administration of NaNO 2 decreased the percentage of the normal hepatocytes; however, the administration of ethanol extract of red okra to the mice exposed to NaNO 2 increased the percentage of the normal cell compared to the mice given NaNO 2 alone. e administration of NaNO 2 in SN significantly caused an increase in the hepatocytes' swelling percentage compared to the WA. e administration of red okra pods ethanolic extract in P2, P3, and P4 significantly decreased the percentage of swelling compared to SN. ere was no significant difference in the percentage of cell swelling between P1, P2, and P3 as well as between P1 and SN (Figure 2(c)).

Discussion
e activities of CAT and SOD were significantly increased in mice given red okra extract. e same trend of the decline in the activity of the antioxidant enzymes after NaNO 2 exposure was reported by Salama et al. [29] and El-Nabarawy et al. [12]. is fall in the antioxidant enzymes' activities may signify the decrease of the antioxidant activity due to the oxidative stress caused by NaNO 2 . NaNO 2 has inhibitory effects on the SOD and CAT enzymes and other antioxidant enzymes in erythrocytes [30,31]; hence, the levels of O 2 and hydrogen peroxide (H 2 O 2 ) are elevated and cause oxidative stress [7] that can destruct other biomolecules, such as protein, lipid, and DNA [7,11,30,32]. O 2 can irreversibly oxidize non-sulfur centers of the antioxidant enzymes during protein oxidation, leading to their inactivation [33]. Another reason for the decrease in the activities may be the direct reaction of the antioxidant enzymes' metal cofactors (copper, iron, zinc, and manganese) with NO produced from NaNO 2 to form complexes [30]. e increase of the SOD and CAT in the liver after the administration of ethanol extract from red okra pods was also reported in the study of Saravanan et al. and Hu et al. [3,34]. In another study, okra flavonoid content caused an increase in the activity of SOD previously jeopardized by oxidative stress in fatigue-induced male mice compared to the control [35]. It was explained to support these results that okra pods have flavonoid compounds such as anthocyanin and quercetin that act as antioxidants [21,22,36] which scavenge free Different superscript letters within each column indicate a significant difference between the means (p < 0.05). SOD: superoxide dismutase, CAT: catalase, MDA: malondialdehyde, NO: nitric oxide, WA: mice were given water alone, SN: mice were given NaNO 2 alone. P1, P2, P3, and P4: 25, 50, 75, and 100 mg/kg BW red okra pods extract and 50 mg/kg BW NaNO 2 , respectively. Different superscript letters within each column indicate a significant difference between the means (p < 0.05). ALT: alanine transaminase, AST: aspartate transaminase, ALP: alkaline phosphatase, TSP: total serum protein. WA: mice were given water alone, and SN: mice were given sodium nitrite alone. P1, P2, P3, and P4: 25, 50, 75, and 100 mg/kg BW red okra pods extract and 50 mg/kg BW NaNO 2 , respectively.    Veterinary Medicine International radicals [35] by donating their electrons to the oxidants [37] and hence stop the destructive chain reaction initiated by the free radicals [20]. Also, flavonoids can promote the activity of the antioxidant enzymes by activating the enzymes [38]. e activation involves various signaling cascades where the phytochemicals from red okra induce the binding of Kelch-ECH associated protein 1 (Keap1) to the nuclear erythroid 2related factor 2 (Nrf2), leading to its stabilization, and therefore binds to antioxidant response element (ARE). is action is essential in activating the genes of the antioxidant enzymes [39,40]. Liao et al. [41] showed that okra extracts cause upregulation of the SOD gene (SOD 2 ) in type 2 induced diabetes in mice.

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Moreover, flavonoids can prevent the formation of ROS/ RNS. It inhibited the enzymes involved in the production of the free radicals, such as reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOx) and microsomal monooxygenase or can directly chelate metal ions such as iron that are involved in the formation of the free radicals [42], hence preventing oxidative stress that can interrupt the activity of the antioxidant enzymes.
MDA and F2-isoprostanes are essential markers of lipid peroxidation of the polyunsaturated fatty acid (PUFA), especially the arachidonic acid of the lipid bilayer of the cell membrane [4,33]. Both MDA and F2-isoprostanes improved in the SN. e current study indicated an increase in peroxidation of the PUFA [20] due to oxidative stress. NaNO2 causes hypoxia and the production of free radicals [31] that target the side-chain methylene carbon of PUFA to remove a hydrogen atom from it to start a destructive chain reaction [43]. e decrease of the MDA and F2-isoprostanes in the red okra-treated mice, especially those given 100 mg/ kg BW indicated a decrease in lipid peroxidation. e flavonoid from red okra contains three rings A, B, and C [44].
e B ring has a 3′-4′-catechol structure with a higher affinity to metal ions [42] responsible for lipid peroxidation [44], so binding the metal ions to the flavonoids prevents the metal ions from catalyzing lipid peroxidation. e decrease of MDA and F2-isoprostanes may be the red okra pods ethanol extract's role to give their electrons to the free radicals to arrest PUFA peroxidation propagation [45].
e increase of NO in SN is comparable to the findings from other studies where administration of 0.2% NaNO 2 for six weeks caused an increase of serum NO concentration in male albino rats [46]. e increase indicated the presence of oxidative stress and hypoxia. When there is a mismatch Veterinary Medicine International between the oxygen demand of the cells and the amount of oxygen available in the cell, inducible nitric oxide synthase (iNOS) is overexpressed to produce more NO after being promoted by hypoxia-inducible factor-1 (HIFα-1) [47,48]. Oxidative stress causes inflammation; during inflammation, iNOS that is highly expressed in the inflammatory cells, such as macrophages, is activated to produce NO that can interact with O 2 and O 2 to produce ONOO, which cause DNA deamination and protein nitration, hence accelerating more inflammation [48] and damage of other biomolecules [49]. During inflammation, neutrophils can activate myeloperoxidase to convert H 2 O 2 produced from superoxide conversion by SOD to form hypochlorous acid (HOCl), a highly toxic substance [48]. Generally, the increase of NO concentration in mice administered with NaNO 2 alone signifies the induced production of NO from iNOS. e decrease of NO concentration in mice given various concentrations of red okra pods extract compared to SN is in harmony with the study of Luo et al. [40], where flavonoids from okra flowers caused a decrease in the concentration of NO compared to the mice model with a cerebral ischemia-reperfusion injury caused by oxidative stress in the brain. Flavonoids can inhibit the synthesis of NO by downregulating the proinflammatory cofactors of iNOS induction [50]. e formation of NO during NaNO 2 exposure depends on oxidative stress. erefore, the best mechanism to prevent NO formation and subsequent damaging molecules such as HOCl and ONOO is to scavenge free radicals such as O 2 and H 2 O 2 [48]. In the current study, the increase of NO in the liver homogenates of NaNO 2 -treated animals was associated with a fall of SOD and CAT activities, while the decrease of NO levels in red okra pods-treated mice was associated with the increase of the antioxidant enzymes (Table 1). It may have confirmed that the induction of NO production facilitates free radicals, which decrease antioxidant enzymes' activities (SOD and CAT). e decrease in NO level may have confirmed the decrease in oxidative stress due to SOD and CAT activities, which have foraged O 2 and H 2 O 2 . NaNO 2 significantly caused an increase in ALP activity in the SN by approximately two folds compared to WA. Compared to SN, the administration of ethanol extract of red okra significantly reduced the ALP activity in all mice given the extracts. e increase of the liver enzyme activities in NaNO 2 mice agrees with several studies [5,6,11,29]; this increase indicates liver damage caused by oxidative stress, which destroys the cellular membrane leading to the discharge of the enzymes from the liver to the blood because of the increased penetrability. NaNO 2 causes hepatocyte destruction through oxidative stress produced due to hypoxia, nitroso compound formation, or other metal-catalyzed reactions. e oxidative stress causes many destructions in the various parts of the cell, including the main target of free radicals, the cellular membrane by lipid peroxidation to decrease the plasma membrane's integrity [5] and destabilize it [6]. When the hepatocytes' cell membrane is damaged, the contents of the liver's cells, including AST, ALT, and AST, are released into the blood hence increases their activities in the serum. In this study, the serum AST, ALT, and ALP activities generally decreased in the mice given the extract compared to the SN. ese results may show that the flavonoid compounds from okra earlier described by Khomsug et al. [36], Anjani et al. [22], and Irshad et al. [21] restored the normal activities of the enzymes at the highest dose (100 mg/kg BW). e results are corroborated by the study of Alqasoumi [14] and Hu et al. [34], where the administration of various doses of ethanol and methanol extracts of okra caused a decrease in the activities of liver enzymes in carbon tetrachloride-(CCl 4 -) induced oxidative stress. e reason for the decrease in the activity of the enzymes in the group given 100 mg/kg BW NaNO 2 with various doses of ethanol extract from red okra may be the stabilization of the liver cells membrane by flavonoids to maintain the integrity of the membrane of the liver cells and protect it from the NaNO 2 -facilitated hepatotoxicity.
e TSP is an essential indicator of tracing liver diseases caused by oxidative stress. e results of a decrease of TSP in the SN compared to the WA in the current study agree with Hassan et al. [6], Aboulgasem et al. [51], and Adewale et al. [52] who reported an elevated concentration of TSP in male albino rats (Rattus rattus), Wistar rats, and guinea pigs, respectively, treated with NaNO 2 compared to the normal groups. e decrease may be due to the increased deamination of amino acids in the liver [53], the formation of nitroso compounds, and the inhibition of protein synthesis due to the inhibition of oxidative phosphorylation NaNO 2 as the result of hypoxia. Saravanan et al. [3] clarified that protein synthesis inhibition might occur through ribosomal RNA hypomethylation leading to weakening lipid and protein synthesis processes. In general, the decrease in TSP may indicate the liver's failure to protect the body from oxidative stress caused by NaNO 2 . e increase of TSP concentration in the okra-treated group compared to the SN is concomitant with Alqasoumi [14]. is increase in TSP concentration may indicate the hepatoprotective potential of ethanol extract of red okra pods from the oxidative stress induced by NaNO 2 exposure.
Usually, necrosis is accompanied by inflammation [54]. Sodium nitrite can cause a rapid depletion of adenosine triphosphate (ATP); hence, the cell membrane loses some functional properties, allowing extracellular fluids and ions. e incursion of the fluid and solutes such as sodium ions causes cytoplasm vacuolation and degeneration, swelling of the cell and organelles, and ultimately rupture of the cell membrane and, therefore, causes cell death by necrosis [55][56][57]. During necrosis, the contents of the cells (damageassociated molecular patterns (DAMPs)) such as histones are released due to the ruptured cell membranes, hence causing nonadaptive and acquired immune responses, including the formation and buildup of extracellular neutrophils in the tissue [58,59]. e release of histones induces several inflammatory response cascades, such as caspase release-1 that causes cell swelling, rupture of the cell membrane, and other intracellular proinflammatory substances the accelerate liver injury. Generally, the Kupffer cells promote proinflammatory cells, monocytes, and neutrophils to the liver tissue [58].
Also, the inflammation in the liver tissue may be caused by hypoxia. NaNO 2 causes a mismatch between the supply and demand of oxygen, causing a surge in ROS production 8 Veterinary Medicine International that mediates inflammation [58,60]. During hypoxiation, HIF-1α is overexpressed and translocated to the nucleus to bind with the HIF-1β and its cofactor p300/CBP. Overexpression of HIF-1α enhances the inflammatory T helper type 1 ( 1) and 17 ( 17); hence, the amount of interferongamma (IFN-c) and interleukin-17 increases. Also, HIF-1α was found to potentiate the effect of interleukin 1β and tumor necrosis factor-alpha (TNF-α). e HIF aims to make the cell more glycolytic and produce ATP efficiently, hence producing lactate that enhances inflammation by boosting interleukin-6 and interleukin-23 from macrophage and succinate activates the macrophages [57]. e accumulation of macrophage around the portal area and in the parenchymal hepatocytes is associated with cells' mechanism to eliminate toxic materials to preserve the normal metabolic activities [60]. e administration of the NaNO 2 , together with doses of ethanol extract of red okra pods, showed some improvement from the damage caused by NaNO 2 . e P4 group showed the best improvement from the cellular damage and inflammation compared to the other okra-treated mice. ese results are in harmony with the biochemical observations, which showed improvement of the antioxidant system of the liver and liver functions in red okra-treated animals. ese findings from the microscopic examination of the hepatic tissue agree with those of Wahyuningsih et al. [20], where the administration of methanol extract from green okra caused a decrease in the percentage of necrotic cells in the kidney in male BALB/c mice exposed to lead acetate. In another study, Alqasoumi [14] found a decreased inflammation and necrosis in the liver tissues of rats administered with CCl 4 and okra extract compared to the group given CCl 4 only. Moreover, our current findings are supported by Al-Rasheed et al. [59], who reported that flavonoids could chelate cellular iron ions to inhibit the ubiquitination of HIF-1α.

Conclusion
is study shows that the administration of NaNO 2 to mice causes damage to the antioxidant system and the liver. e administration of red okra extract has proven to possess protective potential on the antioxidant system and liver by increasing SOD, CAT, and MDA and reducing F2-isoprostanes and NO. Red okra extract succeeded in reducing the biochemical parameters of liver damage (aspartate aminotransferase, alkaline phosphatase, and alanine aminotransferase), reducing the number of necrotic liver cells and reducing swelling and hepatocytes to normal. erefore, it can be concluded that ethanol extract from red okra has antioxidant and hepatoprotective activity against liver injury induced by sodium nitrite. Hence, we recommend the red okra pods as affordable medicines and a natural antioxidant source to protect the liver against NaNO 2 toxicity.

Data Availability
All crucial data used to support the findings herein are included within this article.

Conflicts of Interest
e authors declare that they have no conflicts of interest regarding the publication of this paper.