The Effects of Lactobacillus farciminis and Lactobacillus rhamnosus on Growth, Blood Biochemical, and Meat Quality Indicators of Specific Pathogen-Free Broiler Chickens

The aim of our study was to evaluate the effects of Lactobacillus farciminis and Lactobacillus rhamnosus on live weight gain, feed consumption indicators, and some metabolic blood biochemical and meat quality indicators of specific pathogen-free Ross 308 broiler chickens. We carried out the study in three trials and included a total of 780 unsexed Ross 308 chickens, which we randomly divided into two groups: the control group (Con, n = 390, basal diet) and the probiotic group (ProL, n = 390, basal diet + a powder consisting of L. farciminis and L. rhamnosus 4 g/10 kg of feed). We raised broilers until day 35. We determined the amount of feed consumed, the average daily weight gain, the feed conversion ratio, the average daily feed intake, and the cumulative feed intake once a week. We collected blood samples from 45 broilers from each group at the end of the study. In addition, we slaughtered 30 broilers from each group by cervical dislocation to obtain a breast muscle sample (without skin) to determine meat quality in these chickens (cholesterol and unsaturated, omega-3, omega-6, omega-9, and saturated fatty acids). Feeding a probiotic mixture containing L. farciminis and L. rhamnosus did not significantly affect the growth and feed intake indicators. Feeding these probiotics significantly lowered the blood serum cholesterol levels but did not provide the expected reduction in meat cholesterol levels. However, feeding a probiotic mixture increased the levels of polyunsaturated fatty acids (omega-3 and omega-6 fatty acids) in the breast meat and decreased saturated fatty acids. To better explain the effect of the combination of lactic acid bacteria (L. farciminis and L. rhamnosus) on the growth and development of broiler chickens in our study, histological and immunohistochemical examinations should be performed.


Introduction
Probiotics are living microorganisms that, when administered in adequate amounts, improve the health of the host. Other defnitions have been developed over the years that specify their mechanisms of action, site, delivery format, method, or host [1]. Te positive efects of probiotics on the body have been widely studied in both human and veterinary medicine. Te use of probiotics in farm animals has become an important research topic since 2006, when the use of antibiotics for disease prevention and productivity stimulation was banned in the European Union [2]. Poultry industry producers face various problems such as a reduced growth rate, dysbacteriosis of the digestive tract, and enteritis caused by various pathogens [3,4]. Solutions to these and other problems are still being sought, and research has been conducted to prove the positive efects of probiotics on the host.
Te efects of probiotics on growth performance have been widely studied. For example, Hussein and Selim [5] reported that feeding multistrain probiotics to broilers signifcantly reduced daily feed intake and improved feed conversion compared with the control group. Similarly, Chen et al. [6] fed Lactobacillus rhamnosus to broilers (white leghorns) and observed a signifcantly increased live weight and average daily gain as well as a signifcantly decreased feed conversion ratio (FCR) at the end of the study. However, Qorbanpour et al. [7] fed male Ross 308 broilers multistrain probiotics (including Lactobacillus acidophilus and Lactobacillus casei) and showed that the experimental diet had no efect on daily feed intake, weight gain, and overall feed conversion throughout the study period. Zhu et al. [8] obtained similar results: feeding female broilers probiotics consisting of thermally inactivated Bacillus subtilis and L. acidophilus in a 1 : 1 ratio, the authors reported a signifcantly reduced FCR but found no signifcant differences in parameters such as fnal body weight, average daily gain, and average daily feed intake between groups. Tese contradictory results indicate that additional studies in this feld are necessary.
Te impacts of probiotics on biochemical indicators of protein and fat turnover in the blood of birds are still subject to debate. In several studies, broilers fed probiotics containing various Lactobacillus spp. presented signifcantly higher amounts of total protein, including albumin and globulin, as well as signifcantly lower concentrations of cholesterol, total lipids, and triglycerides in the blood [5,9,10]. However, in several studies, when broilers were fed probiotics that included Lactobacillus spp., there were no signifcant changes in the biochemical blood parameters at the end of the study [7,8,11].
Currently, consumers demand cheap but high-quality and nutrient-rich food. Terefore, producers and researchers have focused on improving not only feed conversion and bird productivity, but also the quality of the obtained products, including poultry meat. According to previous studies, when broilers are fed probiotics, the waterbinding capacity of the meat is increased [4,12], along with the percentages of moisture, protein, and ash in meat. In contrast, the fat content is reduced [13,14], along with the cholesterol content [13,15]. In poultry meat, the total amounts of saturated fatty acids (SFAs) and unsaturated fatty acids and their ratio are important quality indicators. Poultry meat contains long-chain polyunsaturated fatty acids (LC-PUFAs) and is a source of omega-3 (ω-3), omega-6 (ω-6), and omega-9 (ω-9) fatty acids. Various studies have shown that probiotic feeding can increase the levels of PUFAs and reduce the levels of SFAs in poultry meat, thereby afecting the ratios of LC-PUFAs and SFAs [16,17]. Alagawany [18] reviewed the results regarding various sources of ω-3 and ω-6 fatty acids and concluded that producers and scientists, using various vegetable oils or products of fsh origin as feed additives, often face the problem of oxidative stability of the obtained poultry meat. LC-PUFAs in meat are highly susceptible to oxidation, resulting in of-favours and of-odours in poultry meat, which negatively afect meat quality and consumer acceptability. Te authors acknowledged that it is necessary to continue looking for ways to increase the amount of ω-3 fatty acids in poultry meat and to bring the ω-6/ω-3 ratio closer to 1-4/1 [18].
Our research has shown that a feed supplement containing L. farciminis and L. rhamnosus activates the internal reserves of the alimentary canal of broiler chickens and facilitates the digestion of industrially produced feed. Tis would allow increasing the quantitative and qualitative indicators of the meat, without harming or perhaps even improving the health status of fast-growing broilers [19]. Te aim of our study was to determine the efects of L. farciminis and L. rhamnosus on live weight gain, feed consumption indicators, and some metabolic blood biochemical and meat quality indicators of specifc pathogen-free Ross 308 broilers. All trials were identical, and the results for each relevant group were combined and analysed. In total, 780 (260 in each trial) specifc pathogen-free Ross 308 broilers (males and females) were obtained after hatching from the commercial hatchery "Kekava" (chicks were not infested with any specifc pathogens). Te broilers were weighed and randomly divided into two groups: the control group (Con, n � 390 (130 in each trial)) and the probiotic group (ProL, n � 390 (130 in each trial)). Te groups were placed in two identical rooms (biochambers) with full microclimate control (temperature, humidity and air supply, control of incoming and outgoing air composition, and light mode) and video surveillance. Te volume of each room was 9 m 3 . Te birds were placed on deep bedding, using clean pine and spruce shavings as bedding material. In both groups, during the frst week, the ambient temperature in the chambers was maintained at 32-34°C, which, as the birds grew, was gradually reduced to reach 20°C by the end of the study. Te daily light regime on the frst day was 23 h of light and 1 h of darkness (23 h/1 h). After that, the dark regime was gradually extended, and from days 7 to 26, the light regime was 18 h light and 6 h dark (18 h/6 h). In the last week of the study, the dark regime was gradually reduced until it reached 20 h of light and 4 h of dark (20 h/4 h).

Diet and Supplementation of Lactobacillus spp.
Fresh drinking water was provided ad libitum in the drinking lines. Te basal diet for both groups (Con and ProL) was the same and was composed according to the age of the chicks: starter (from day 0 to 10), grower (from day 11 to 24), and fnisher (from day 25 until the end of the study). Te analytical composition and physiological additives of the diet at each stage of feeding are shown in Table 1. Te ration was prepared based on the Ross 308 breeding guidelines [21]. Te starter diet contained wheat grains, soy sprouts, vegetable oil, corn gluten, monocalcium phosphate, fsh meal, calcium carbonate, sodium chloride, and sodium sulphate. Te grower and the fnisher diets contained no fsh meal, but they did contain rapeseed and fatty acids.
We added a powder containing lactic acid bacteria to the feed of the ProL group, consisting of L. farciminis (CNCM-I-3699-7.8.106 GU/g) and L. rhamnosus (CNCM-I-3698-7.8.106 GU/g), at estimated doses of 4 g per 10 kg of feed (initially mixing the probiotic powder into 1 kg of feed, then mixing it with the rest of the daily feed ration for 5 minutes), according to the conditions provided by the manufacturer (STI Biotechnologie, France) [22]. Te mixture was stored and used exactly as recommended by the scientists involved in the production.

Measurements of Growth
Performance. Te amount of feed ofered to each groups (Con and ProL) was the same. Specifcally, feed was ofered twice a day in feeders with suitably sized openings, reducing the possibility of the birds scavenging it in the litter. Te amount of feed consumed by each group was determined once a week by weighing the amount of uneaten feed left in the feeders of each group. Based on the weight of the remaining and ofered feed, the weekly average amount of feed consumed per day and the cumulative feed intake (CFI) were calculated.
Te live body weight (LBW) for each bird in each group was determined on the day of placing the bird and then once a week, on days 7, 14, 21, 28, and 35. Te average daily live weight gain (ADWG) of chickens and the FCR were determined using the formula provided by Chen et al. [6] in the following consecutive study periods: days 1-7, 8-14, 15-21, 22-28, and 29-35. Mortality was recorded once a day; the body weight of the bird and the cause of death were determined. It should be noted that chicken mortality during the study was lower than that under Latvia's production conditions (∼2%-5%).

Blood Sample Collection and Examination.
According to our research methodology, and based on similar previous studies [10,11], blood samples were collected from the wing vein of 45 randomly selected broilers of each group at the end of the study. Te samples were examined in the laboratory of the Veterinary Clinic of Latvia University of Life Sciences and Technologies, using a Mindray BS200 Clinical Analyzer, with the absorbance photometry method. For this study, the following indicators were analysed: cholesterol (mmol/L), triglycerides (mmol/L), uric acid (mmol/L), total protein (g/L), albumin (g/L), and alkaline phosphatase (ALP, U/L).

Meat Sample Collection and Examination.
To obtain a breast meat sample (without skin) that could refect the production conditions, the broilers were stunned using the cervical dislocation method and bled. Tis procedure was carried out by a certifed butcher; hence, the meat samples obtained during the study and the determined indicators are identical to meat that would be obtained under the production conditions. We obtained samples for meat quality examination from 30 randomly selected broilers of each group at the end of the study. After obtaining the samples, they were cooled, placed in polyethylene packaging intended for laboratory samples, and frozen at −22°C. Meat samples were investigated in the laboratory of the Scientifc Institute of Food Safety, Animal Health, and Environment "BIOR" (Riga, Latvia). Te following parameters were determined: cholesterol (mg/100 g), using method BIOR-T-012-132-2011, and PUFAs (g/100 g), ω-3 fatty acids (g/100 g), ω-6 fatty acids (g/100 g), ω-9 fatty acids (g/ 100 g), and SFAs (g/100 g), using method BIOR-T-012-131-2011. Te PUFA/SFA and ω-6/ω-3 ratios were determined.

Statistical Analysis.
We assessed the data distribution by using the Shapiro-Wilk test and by inspecting the normal Q-Q plots. We used Levene's test to test for homogeneity of variances. We determined the diferences between the Con and ProL groups by using the independent samples t-test because the data were normally distributed and had homogenous variances. Te results are presented as the mean ± standard deviation unless otherwise stated. We used Microsoft Excel to visualise the data and the Jamovi program [23] for statistical analysis. We considered p < 0.05 to be a statistically signifcant diference.

Growth Performance.
Since the ban on the prophylactic use of antibiotics, researchers have evaluated the potential of probiotics to improve growth performance in commercial Veterinary Medicine International poultry farms [24]. Probiotics improve chicken growth by participating in the processing of nutrients and by modulating the intestinal microfora and improving the barrier function of the intestinal walls. Probiotics lead to competitive exclusion of pathogenic bacteria as they compete with probiotics for nutrients, colonise the intestinal surface, leaving no attachment points for pathogenic microfora, and stimulate the immune system [25,26]. Te LBW, ADWG, CFI, and FCR did not difer signifcantly between the Con and ProL groups (p > 0.05) ( Table 2). To exclude the infuence of the environment, pathogens and other stressors on the results, we tried to provide the optimal keeping conditions for the broilers of both groups throughout the study. Both groups performed extremely well in all phases of the study. We noted such high LBW and ADWG using lactic acid bacteria ofered by the same manufacturer in only one study [22]; more often, LBW and ADWG were lower compared with the results of our study [6,10,27,28]. However, when analysing the dynamics of the indicators, the curves of live weight and cumulative feed consumption initially increased slowly (from days 1 to 14), followed by a rapid increase in live weight and feed consumption (from days 15 to 35). Tis is the period when birds consume more food and grow faster. On the other hand, ADWG was initially higher (from days 1 to 28), mainly because, from their initial small starting weight, weight gain was faster; within a week, the chickens had doubled or even tripled their weight. Te feed conversion curve increased gradually: as the birds grew larger, they consumed more feed. However, because the feed use efciency was high, we did not observe a rapid increase in feed conversion.
Overall, there were very good production ratings in both study groups, and there were no signifcant diferences between the groups. Tis could be explained by the fact that under favourable conditions, where the bird is not exposed to the risk of disease and stress; as in our study, the addition of probiotics to the feed may not give the expected results. Tis shows the signifcance and importance of microclimate and housing for broiler production and development [29,30].
Similarly to our study, Sugiharto et al. [31] fed Lohmann broilers probiotics at diferent concentrations for 42 days and observed no signifcant changes in growth indicators (live weight, cumulative feed consumption, and the FCR), neither in individual study periods nor throughout the entire study period. Sarangi et al. [32] also found a similar dynamic in a 42-day study when they fed Vencobb broilers a probiotic mixture consisting of Lactobacillus bulgaricus and Lactobacillus plantarum. Te authors reported a signifcantly higher live weight on day 14 of the study, but at the end of the study, there were no signifcant changes in live weight. In that study, the authors found no signifcant diferences between the groups in indicators of cumulative feed consumption. Tey found a signifcantly lower FCR in the probiotic group between weeks 4 and 6 of the study, but in general, there were no signifcant changes between the groups [32].
Other studies have reported better results using probiotics. In their 21-day study, Agustono et al. [33] fed ISA brown male broilers a probiotic mixture consisting of L. acidophilus, L. plantarum, and Bifdobacterium spp. in diferent concentrations. Tey reported a signifcantly higher live weight in all groups that were fed probiotics. At the same time, there was signifcantly higher feed consumption; however, the FCR remained signifcantly lower in the study groups, indicating good feed use efciency. Chen et al. [6] reported better results after feeding broilers (white leghorns) with L. rhamnosus. At the end of the study, the authors observed a signifcantly increased live weight and average daily gain and a signifcantly reduced FCR (p < 0.05) compared with the group that was not fed probiotics. In several studies, the authors achieved signifcantly better results by feeding probiotics for 42 days. Palamidi et al. [3] fed male Cobb broilers a poultry-specifc multispecies product consisting of fve probiotic isolates (including Limosilactobacillus reuteri isolated from the hump) during the fnisher period (days [29][30][31][32][33][34][35][36][37][38][39][40][41][42]. Troughout the experimental period, the chickens achieved a signifcantly higher body weight gain and a signifcantly lower FCR, irrespective of the addition of live and inactivated forms of probiotics. Furthermore, in a 42-day study conducted by STI Biotechnologie [22], when broilers received a probiotic feed supplement containing L. rhamnosus and L. farciminis in diferent concentrations, and the authors observed a significantly reduced daily feed intake and a reduced FCR compared with groups that did not receive this supplement [22].

Blood Biochemical Indicators.
Blood biochemical indicators provide extensive information on the animal's state of health. However, they can be afected by numerous factors: species, age, sex, housing conditions, nutrition, seasonal changes, and geographical region [34,35]. To analyse serum blood biochemical indicators, we searched for scientifcally confrmed data with defned normal physiological limits for broilers at the age of 30-40 days, but we could not fnd such data. Hence, we used the following studies with a neutral design and investigations that did not specify any other factors that infuenced the results. Café [34] obtained blood biochemical data from broilers grown under thermoneutral conditions. Angoua Kokore et al. [35] compared blood biochemical indicators of local and meat broiler breeds. Shuvo et al. [36] evaluated the efect of 35 days of Lactobacillus supplement feeding on broiler chickens, including blood parameters.
We found that almost all parameters (Table 3) did not difer signifcantly (p > 0.05) between the Con and ProL groups, except for cholesterol (p � 0.039), with a diference of 0.26 mmol/L (95% confdence interval (CI) 0.01-0.51, d � 0.45 (medium efect size)). Te cholesterol level in the study by Café [34] was 3.25 mmol/L, which is 7.14% lower than that in the ProL group of our study and 13.56% lower than that in the Con group. In the study by Shuvo et al. [36], the average cholesterol level in the control group was only 2.74 ± 0.06 mmol/L, but in the study group, it was even lower (2.12 ± 0.12 mmol/L). Te authors explained this outcome by referring to the presence of lactic acid bacteria in the fermented feed and the ability of these bacteria to collect and bind cholesterol. As a result, the total cholesterol level in the blood serum would decrease due to the inhibition of bile acid absorption in the intestine [36,37]. Mohan et al. [38] also explained the ability of acidophilic bacteria to decrease cholesterol levels in the blood by reducing its absorption and/or synthesis in the intestines.
Cholesterol is an important component of cell membranes: it ensures cell elasticity and membrane permeability and participates in the synthesis of important hormones (e.g., sex hormones and hormones of the adrenal cortex) and bile acid; a small amount of it is also needed for the synthesis of vitamin D [39]. Although we found a signifcantly lower blood cholesterol level in the ProL group, it is still higher than the levels reported in previous studies. We would like to add that there is no reason to consider the blood cholesterol level we found to be elevated (above the physiological norm) because the animals in our study were in good health, as evidenced by the high productivity rates.
Similarly to our research, Hashemzadeh et al. [40] fed male Ross 308 broiler chicks L. rhamnosus for 42 days and reported a signifcantly lower blood cholesterol level. Ghorbani et al. [41] also achieved similar results in a 42-day study by feeding the commercial probiotic "Primalac" to Ross 308 broilers; at the end of the study, the blood cholesterol level was signifcantly lower than that in the control group. Yazhini et al. [42] also reported positive results in a 42-day study in which they fed broiler chicks nonencapsulated and encapsulated Lactococcus lactis and Bifdobacterium bifdum; in all probiotic groups, the authors observed a signifcantly lower total cholesterol level. In addition, Hussein and Selim [5], fed broilers probiotics, including L. acidophilus and found that the total blood cholesterol level decreased signifcantly compared with the group that did not receive probiotics. However, Zhu et al. [8] described opposite results. Female yellow-feathered broilers fed heat-inactivated compound probiotic in a 63-day study  showed a signifcantly reduced blood cholesterol level on day 42 of the study, but no signifcant diference on day 63. Sugiharto et al. [43] also reported that there was not a signifcant reduction in the blood cholesterol levels in any of the study groups of Indonesian indigenous crossbred chicks in a 10-week study by feeding B. subtilis and a multistrain probiotic preparation in combination with vitamins and minerals.
Although we did not fnd signifcant diferences in the other blood biochemical indicators, we highlight certain indicators (triglycerides, total protein, and albumin).
Te blood triglyceride concentration was 6.95% higher in the ProL group (0.72 ± 0.31 mmol/L) compared with the Con group (0.67 ± 0.30 mmol/L). In the study by Café [34], the triglyceride concentration was even higher (0.84 mmol/L) on day 35. Triglycerides are some of the most important plasma lipids and are either ingested with food (exogenous triglycerides) or synthesised in the liver (endogenous triglycerides). Tey are stored in adipose tissue, and only a small amount circulates in the blood. If the levels of fatty acids and carbohydrates in the blood are too high, they are converted and stored in the body as triglycerides [39]. Terefore, in our study, given the same feed ratio, the chickens in the ProL group digested the feed more successfully, providing a higher level of triglyceride raw material.
Te level of total protein in the blood was lower in the chickens of the ProL group (33.03 ± 6.49 g/L) compared with the Con group (34.92 ± 7.26 g/L). Café [34] reported a total protein level of 25-45 g/L in blood, but it was higher in the study by Angoua Kokore et al. [35], specifcally 52.2 ± 6.4 g/L. However, the albumin levels in our study were higher in the ProL group (15.45 ± 2.79 g/L) than those in the Con group (15.03 ± 3.01 g/L). Café [34] reported lower albumin levels than either group of our study (12.7 g/L), but Shuvo et al. [36] reported a higher level for the control group (17.8 ± 1.9 g/L). Albumin is a plasma protein that is synthesised in the liver for ingested and absorbed nutrients. It constitutes more than half of the total protein (the second largest fraction is represented by globulins). Albumin maintains colloidal osmotic pressure and acts as a carrier protein for hormones, fatty acids, bilirubin, calcium ions, and drugs, among others. Albumin levels can indicate liver function and protein metabolism in the body [39]. Although there was not a signifcant diference between the ProL and Con groups, a higher level of albumin in the blood indicates more successful liver function and protein exchange in the body.
Other authors have also studied the ability of lactic acid bacteria to positively infuence these blood biochemical indicators in birds. Hosseini et al. [9] fed male Cobb broiler chicks the commercial probiotic mix Protexin, which contains several Lactobacillus spp., including L. rhamnosus, and reported signifcantly higher concentrations of total protein and albumin as well as a signifcantly lower blood triglyceride concentrations compared with the control group and the group to which antibiotics were added to the diet. Hussein and Selim [5] reported similar results: a signifcantly higher concentration of total protein, including albumin and globulin, as well as a signifcantly reduced concentration of total lipids in the blood. In a 35-day study in which broilers were fed diferent concentrations of a probiotic containing L. casei, Astuti et al. [10], achieved signifcantly higher plasma total protein levels in all probiotic groups. However, Qorbanpour et al. [7], Shah et al. [11], and Zhu et al. [8] fed broilers with probiotics, which included Lactobacillus spp. and did not observe signifcant diferences at the end of the study in the blood biochemical parameters compared with the control group. In contrast, Sugiharto et al. [43] found signifcantly lower blood triglyceride and total protein (including albumins and globulins) concentrations (p < 0.05), but no signifcant diferences in the ratio of albumins to globulins.
Because feeding a mixture of L. farciminis and L. rhamnosus for 35 days signifcantly reduced the blood cholesterol levels, we expected that its level in breast meat samples would also be lower in the ProL group. However, the breast cholesterol level was signifcantly higher (p < 0.05) than that in the Con group. According to published studies, the cholesterol level in breast muscle without skin ranges from 77 to 85 mg/100 g [44,45], but in the breast meat samples (also without skin) submitted to our laboratory, it was 12.92 ± 0.87 mg/100 g (ProL) and 10.02 ± 0.35 mg/100 g (Con)-7-8 times lower. According to the fndings of Conchillo et al. [44], freezing the meat samples before examination does not afect their cholesterol level. Terefore, by using age-appropriate feed in chicken breeding, as well as providing the best possible keeping conditions and regulating the light/dark regime, an especially low cholesterol level can be achieved in breast muscle meat (10-13 mg/100 g of product), as shown by the results of our study.
Chicken breast muscle is a valuable source of essential fatty acids, especially ω-3 LC-PUFAs [46]. ω-3 and ω-6 LC-PUFAs have been studied to help prevent and improve the outcome of chronic diseases in humans, including metabolic, cardiovascular, and neurodegenerative diseases as well as certain types of cancer [18,46,47]. In our study, the ω-3 fatty acid level was 16.25% higher in the ProL group than in the Con group, although this diference was not signifcant (p � 0.104). However, the ω-6 fatty acid level was signifcantly higher in the breast muscle of the ProL group (p � 0.001). In this sense, the addition of L. farciminis and L. rhamnosus to the feed of broilers increased both ω-3 and ω-6 fatty acids in breast meat, without using other feed additives containing, these fatty acids (fsh or other oils) and without producing an unpleasant taste and smell [18]. It is important to note that the intake of ω-3 and ω-6 fatty acids is important to maintain human health. In recent years, concerns regarding the potential role of ω-6 fatty acids in infammation, thrombosis, and low-density lipoprotein oxidation have been dismissed, and it is recommended that at least 5%-10% of the daily energy intake should come directly from ω-6 fatty acids [48].
Researchers are also interested in the ω-6/ω-3 ratio in the human diet, as deviations contribute to the pathogenesis of cardiovascular disease, cancer, infammation, and many autoimmune diseases [18,47]. As food availability and eating habits change, the ω-6/ω-3 ratio in human diets today ranges from 10/1 to 20/1, in contrast to the 1/1 ratio found in our ancestors' diets [49]. According to our results, this ratio was 8.54 ± 2.03 in the ProL group and 8.64 ± 2.41 in the Con group, which is less (and therefore healthier) than that in the modern human diet [49].
After 35 days of consuming a feed supplement containing lactic acid bacteria, SFAs also decreased signifcantly in the breast meat samples of the ProL group compared with the Con group (p � 0.031). Given that SFAs are less healthy than PUFAs [47], reducing their amount is a signifcant achievement. Te levels of fatty acids, especially LC-PUFAs, in chicken meat can be modifed more easily than those in other livestock meats [50].
According to the recommendations of the UK Ministry of Health [51] and other authors, it is important to consider the PUFA/SFA ratio [51,52]. Under our housing and feeding conditions, the inclusion of probiotics in the feed of broilers did not signifcantly change the PUFA/SFA ratio: it was 2.68 ± 0.24 for the ProL group and 2.48 ± 0.25 for the Con group. Although the inclusion of a mixture of lactic acid bacteria did not signifcantly afect this ratio, pathogen-free conditions ensured very good quality indicators, which encourage further research in this direction.

. Conclusions
Te probiotic mixture containing L. farciminis and L. rhamnosus added to feed in a 35-day study did not signifcantly afect the growth (LBW and ADWG) and feed intake indicators (CFI and FCR) of specifc pathogen-free broilers. Feeding these probiotics signifcantly reduced the blood cholesterol level but did not produce the expected reduction in meat cholesterol. However, probiotic feeding increased the amount of PUFAs (ω-3 and ω-6) in the breast meat of broilers and decreased SFAs. Te main novelty of the study is that we evaluated the efects of probiotics for specifc pathogen-free Ross 308 broilers. Te combination of L. farciminis and L. rhamnosus afected broiler growth, meat quality, and some blood biochemical indicators. Tese changes mean that the chicks did not have to deal with additional stress factors during intense growth, which proves the direct impact of these bacteria on the chicks. Te positive results, albeit contradictory, show that it is necessary to continue to search for the most suitable combinations, doses, and feeding duration of probiotics in poultry farming for each breed and age. In future studies, histological and immunohistochemical examinations should be performed to evaluate in more detail the efect of the combination of lactic acid bacteria (L. farciminis and L. rhamnosus) on the growth and development of broiler chickens.

Data Availability
Te data used to support the conclusions of this study are available from the corresponding author upon reasonable request.

Conflicts of Interest
Te authors declare that there are no conficts of interest regarding the publication of this paper.